LOCUS       DTVV01000001            2183 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1005274, whole genome shotgun sequence.
ACCESSION   DTVV01000001 DTVV01000000
VERSION     DTVV01000001.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1005274"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(272..760)
                     /locus_tag="EYO86_00005"
     CDS             complement(272..760)
                     /locus_tag="EYO86_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIC41452.1"
                     /translation="MQMGRKGFLSWLSLGWLAFAGATGGFFTVMIRFMFPNVLFEPPQ
                     SFKIGFPDDFTVGKVNTNFKNKHAVWIIRDEEGIYALSTVCTHLGCTPNWLEVEQKYK
                     CPCHGSGFRKTGINFEGPAPRPLERHAIFLADDGQIIVDKTKRYKWEKGEWEIADSFL
                     KI"
     gene            complement(812..1684)
                     /locus_tag="EYO86_00010"
     CDS             complement(812..1684)
                     /locus_tag="EYO86_00010"
                     /inference="COORDINATES: protein motif:HMM:PF13646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIC41453.1"
                     /translation="MSEENTKSVNSSKVYTLYYAFFLIPLIITIFGVMFFFMFKVLTY
                     ETNSPADYLTDVQIGSATKRWQAAYELSKLLSNPEMVPTDAVFKNKMVSIYQHAIHDD
                     PMVRTYMALAMGRTGQQDYGAILIEGLNDEDKGSRLAAIKALGLLAYTPAVKGIQEFA
                     AEKYSNAERLAATISLGSMGDKSIIPILQKLLDDEEPNIRWDAAIALAKLEDPSGVEV
                     IVNLLDRSYFDHFTEVDEEEEAQAILVAIQVSSQFPSKLFVTNLLKLAAFDKNMQIRD
                     LAIKTLDKTYNRKI"
BASE COUNT          636 a          468 c          367 g          712 t
ORIGIN      
        1 tccggggcac accagaccgt ataattgatt tccaaacctg ggattcaccc agttgctcta
       61 acattgattc ttttttatct tttttagcca ttctcttctt gtattttatt ttattttttt
      121 acccattgga aacaaagtct tagtaatcaa tatttcatgt aattttattg gaattctgga
      181 aaactggaat tgtattcatt tcctctaaac aaccaatatt ccattcttcc atttatccaa
      241 tattccatta tttccatttt ttctatcatt cttatatctt taaaaatgaa tccgctattt
      301 cccattcacc cttttcccac ttgtagcgtt tggttttatc cactattatc tgtccatcat
      361 ccgcaagaaa aattgcgtga cgttccaagg ggcggggagc agggccttca aaattgatgc
      421 cggttttccg aaagccgctg ccatggcagg ggcatttata tttctgttct acttccagcc
      481 aattgggggt acagcctaaa tgagtgcaaa cagtagatag ggcataaatt ccttcttcat
      541 ctcttataat ccacacagca tgtttatttt taaaattggt atttaccttg cccactgtaa
      601 aatcatcggg aaaaccgatt ttgaaggatt gaggcggttc aaatagtaca ttgggaaaca
      661 tgaaccgaat cataaccgtg aaaaaaccgc ctgttgctcc ggcaaatgcc agccaaccca
      721 gcgagagcca gcttaaaaat ccttttctgc ccatttgcat ttcagaaccg gatttaattg
      781 ccgcctgtgg aataaatttt tcttctgcca attatatttt cctgttataa gttttgtcca
      841 atgttttaat ggcaagatct cttatctgca tatttttatc aaatgcggcc agttttaaaa
      901 gattagtaac aaatagtttt gaaggaaatt gggatgaaac ttgtatggca accaggattg
      961 cctgggcctc ttcctcctca tctacctcgg taaaatgatc aaagtaggat cggtccagca
     1021 gattaacaat tacctcaacg ccggatggat cttccagttt tgccagcgct attgctgcat
     1081 cccagcggat attgggttct tcatcatcaa gtaatttttg taaaatcggt atgatggatt
     1141 tatcacccat gcttcccagg gatattgtgg ctgccagcct ttccgcattt gaatactttt
     1201 cagcggcgaa ttcctgaatc ccctttaccg ctggagtata tgccaaaagc cccagggctt
     1261 taattgctgc caatcggctt cccttatcct catcattcag gccctcaatt aatatagccc
     1321 cataatcctg ctgcccggtt cgtcccatag ccaacgccat ataggtacgc accatgggat
     1381 catcgtgaat agcatgttgg taaatggaga ccattttgtt tttgaagact gcatcagtag
     1441 gcaccatttc aggattagac agtaattttg aaagctcata ggccgcctgc caacgcttgg
     1501 tggctgatcc aatctgtaca tcggtgagat aatctgcagg tgaattggtt tcataggtga
     1561 gtactttgaa catgaaaaag aacataaccc caaaaatggt aataatcagg gggatgagaa
     1621 aaaaagcata atacaaggta taaaccttgc tgctatttac actttttgta ttttcttcac
     1681 tcattagttt ttacgttttt tgcggaattt tccctaaagt ccaagtcatt aatttaaagg
     1741 tattctaaat tatggagaaa agagttattt acaggatttt ttattttacg atctaattat
     1801 tctgattttg ttttgcatca tttttttcaa gcaaatgttt aacgattcca ttcagtttat
     1861 ttacttcctt tttcagttta attacttcac tgagaacaaa aaaactaaaa ataccaaaac
     1921 aaagtacaaa cagggtaagt aattccatta ctgttttacc tccttttcac ccagtttcct
     1981 gaattcaatg gaaaggagta ctggaaataa aagaagtatc cccaaactga tgaacattcc
     2041 caaacctacc cattccagat ttttcatgag ccaatccaat gtgagccagt caaacatttc
     2101 cattcaccat caaaaaacct ttagcaccag ccaaagataa actaccatta atacagtgca
     2161 aaaaatcatg gaaatccatt taa
//
LOCUS       DTVV01000002            1998 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1010827, whole genome shotgun sequence.
ACCESSION   DTVV01000002 DTVV01000000
VERSION     DTVV01000002.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1010827"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="EYO86_00015"
     CDS             complement(<1..916)
                     /locus_tag="EYO86_00015"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:PF03853.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIC41454.1"
                     /translation="MSLNPYIYNQELIQKAERKTFNKTDSFRVMQKAAEACYNTIINT
                     ISTQKILVICGPGNNGGDGILITKHLHDKQKNVSLYAPLGIAKTDDSKKALTLLNNNA
                     LIKQNVNINNFEIIIDSIFGIGLNKPITEELSNLFRSINKTKSKVISIDMPSGVHTDT
                     GRVDSIAVKADITLTFHRLKPGQFLLPGKEYVGEIKLLDIQLENLNNETSIFLNTPLP
                     IKEIEMTDHKYSRGSSYIIAGTQLIGASKLAALAASQSALRSGAGLSKLLIAHDKEEV
                     FKPHILEEMIVLYKNIKDLDLIIKKNKIT"
     gene            969..1709
                     /locus_tag="EYO86_00020"
     CDS             969..1709
                     /locus_tag="EYO86_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIC41455.1"
                     /translation="MKKKNYSNEDGQSIGESVEGWKSCPVPPKTKMEGRYCVIEILDV
                     EKHAEDLFHSFAKDTTDYDWTYLHYGGFKSLIKFKEWLQTECLHNDPLFHVIIDKSQN
                     IAVGMASYMRIQQKIGNIEVGHIHFSPAMQQKTIGTEVMYLMMKRVFDELGYRRYEWK
                     CDSLNERSCQSAKRLGFTFEGIFRQHNIVKGHNRDTAWFSIINKEWNSVKNNYLTWLD
                     KKNFNGAGYQIESLTNLMQSTKKFPFKD"
     gene            complement(1798..>1998)
                     /locus_tag="EYO86_00025"
     CDS             complement(1798..>1998)
                     /locus_tag="EYO86_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HIC41456.1"
                     /translation="QAFQTAIGMSFISMISMEVSMNAVDWIIVGGAEIVWWVVPLMLI
                     VGFLTPWPYNYWRLKKFNLACH"
BASE COUNT          682 a          298 c          330 g          688 t
ORIGIN      
        1 aagttatttt gtttttttta ataattaaat ccaaatcttt tatattttta tacaatacta
       61 tcatttcttc taaaatatgt ggtttaaata cctcttcttt gtcatgtgcg attaatagtt
      121 ttgataatcc tgccccagaa cgcaatgctg actgacttgc agctaaagca gcaagcttag
      181 aagcgccaat taattgagtt ccagcaatga tgtaagaaga tcctcttgaa tatttgtgat
      241 ctgtcatttc tatttctttt atgggcagag gagtattgag aaaaatagaa gtttcgttat
      301 ttaaattttc caattgaata tctagtaatt ttatttctcc tacatattcc tttccgggca
      361 ataaaaattg tccgggtttt aaacggtgaa atgtaagcgt tatgtccgct ttaacagcaa
      421 ttgaatcaac ccgccctgta tccgtatgta cacctgaagg catatcaatg gaaatgacct
      481 ttgatttagt tttatttatg cttcgaaaaa gattacttaa ttcctcagta ataggtttat
      541 ttaagccaat accaaatata ctatcaataa ttatttcaaa attattaata ttaacatttt
      601 gttttataag agcgttattg tttaataaag ttaaagcttt tttggaatca tctgttttgg
      661 ctattccaag gggagcataa agagacacat ttttttgttt atcatgcaaa tgctttgtaa
      721 tcagaatgcc atccccacca ttattcccag gcccacagat aaccaatatt ttttgagttg
      781 agatagtgtt aatgatagta ttataacaag cttctgctgc tttttgcatt acacggaaac
      841 tatctgtttt attaaacgtt tttctttcgg ctttttgaat taattcctga ttataaatat
      901 atggatttaa gctcattatt atttaattat atctcctaaa acaaattagt gataattatt
      961 ttattgtcat gaagaaaaaa aattattcca atgaagatgg ccaatcaata ggtgaatcag
     1021 ttgaaggctg gaagagctgc ccagtgcctc ctaagactaa aatggaagga agatattgtg
     1081 ttatagaaat acttgatgtt gaaaaacatg cagaagatct gtttcattcc tttgcaaaag
     1141 atacaacaga ttatgattgg acatatcttc attatggggg gtttaagtca ttaataaaat
     1201 ttaaagaatg gttacaaaca gaatgtttgc acaacgatcc tttgtttcat gtgataattg
     1261 ataagagtca aaatatcgct gttggtatgg cgagttatat gcgaattcaa caaaagatag
     1321 gcaatattga ggttggacat attcattttt ctcctgctat gcagcaaaaa actattggga
     1381 cagaagtcat gtatttaatg atgaagcggg tttttgatga attaggatat cgaagatatg
     1441 agtggaaatg cgattcttta aatgagcgtt catgccaatc cgctaaacga cttggtttta
     1501 cttttgaggg aatttttaga cagcacaata ttgttaaggg acataatcgc gatacagcat
     1561 ggttttcaat tattaacaaa gaatggaata gtgtaaaaaa taattattta acgtggctag
     1621 ataagaaaaa ttttaacggg gccggatatc aaatagagag ccttacaaat ttaatgcaat
     1681 caactaaaaa atttcccttt aaagattaaa aaatattaat gtagatcatg tgtactttca
     1741 aattgatccg gatcaatttg aatgagctaa ataaaaaaaa taaaaaaaat gtatttttta
     1801 gtgacaagcg agattgaatt tttttaatct ccaataatta tatggccaag gtgttagaaa
     1861 acctactatc agcattaaag ggacaaccca ccatacaatc tctgctccac caacaattat
     1921 ccaatcaaca gcattcatgc ttacttccat actaatcatg gaaataaagc tcatgccaat
     1981 tgctgtttga aaagcttg
//
LOCUS       DTVV01000003            1815 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1013877, whole genome shotgun sequence.
ACCESSION   DTVV01000003 DTVV01000000
VERSION     DTVV01000003.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1013877"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="EYO86_00030"
     CDS             complement(<1..1457)
                     /locus_tag="EYO86_00030"
                     /inference="COORDINATES: protein motif:HMM:PF01464.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41457.1"
                     /translation="MKIYKLITILIALFFISFTNQASVIHLDISKKYKKIFEEKILSE
                     EDVLKYQAIFDLQEECKWKKANKYILEISNNILMGHVLSQRYLHPSCYRSDFVELTHW
                     LKRYNDLPQAKRIYRLAVKRMPQGYKSPIKPIKPIGIQEENLSSKKNSYYQSKKKLSK
                     NQKLEKKKLINAIKSRVNRGWPTGAVKLLQQRDVKLILDQVEIDQQKELIAKGYFLAN
                     KNQLAIQFAEEALLKSAAFVPYAGWTSGLSAWRLGEYEKAAEFFSTFAITLKNDAWHQ
                     AAGSFWAARSYAKLNNYDEINFWINRAAKNPHSFYGLLATKILGIVEPIDWKPTPNNG
                     SLNNKFFQLPSGKRIQALIQIGLPRQLENEIVYMNTVLNKEVALWSLNISQHFNLAYT
                     QLKIVNTLEQYDIILPIKFYYPTPIWQPLNGYTLNPELLYAFMHQESMFNEKAKSHKG
                     AMGLMQVMPSTAKFISSNKKVKKNNGNILKIPEINL"
     gene            complement(1444..>1815)
                     /locus_tag="EYO86_00035"
     CDS             complement(1444..>1815)
                     /locus_tag="EYO86_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIC41458.1"
                     /translation="AINLDRNKVYITNVIPWRPPNNRTPSNEEILQCLPFLQKHIELI
                     KPKLIVLLGAIAAKSVLSTPLGISKLRGRWHEYKSLYLIEPILCLATYHPAFLLRSPG
                     FKKTAWEDLQQLQKKIENENL"
BASE COUNT          549 a          289 c          304 g          673 t
ORIGIN      
        1 agattaattt caggaatttt tagaatattc ccattatttt tttttacttt tttattagag
       61 gaaataaatt tagcggtaga cggcataact tgcattaacc ccattgcacc cttgtgactt
      121 ttggcttttt cattgaacat cgactcctga tgcatgaaag cataaagaag ttctggattt
      181 agcgtgtaac catttaaagg ttgccatatc ggagtgggat agtaaaattt tattggaaga
      241 atgatgtcat actgttcaag tgtattgact atttttaatt gcgtatatgc taaattaaaa
      301 tgttgagaaa tattcagaga ccacaaggca acttctttgt ttaacacggt attcatataa
      361 actatctcat tttctaactg ccgaggtaaa ccgatctgta ttaaagcttg aatgcgtttt
      421 ccactaggta gttgaaaaaa cttgttattc agagatccgt tgttgggtgt aggtttccaa
      481 tcaataggtt caacaatgcc taatattttt gtagctaaca aaccgtaaaa actatgagga
      541 tttttagcgg ccctatttat ccaaaaattt atttcgtcat aattattaag cttggcgtaa
      601 gaacgtgcgg cccaaaaact cccagcggct tgatgccaag catcattttt gagcgtgatt
      661 gcaaaggttg aaaaaaactc tgcagctttt tcatattcac caagtcgcca agcagataaa
      721 ccacttgtcc aaccagcgta gggaacgaat gcagctgatt ttaaaagagc ttcttcagca
      781 aattgaatgg ctaactgatt tttgttagca agaaaatatc ctttagcaat aagttccttt
      841 tgttgatcta tttcaacttg atccaaaata agtttaacat ctctttgctg gagtaattta
      901 actgctcctg ttggccatcc tctatttact cgagacttaa ttgcgttaat taatttcttt
      961 ttttctaatt tttgattttt agatagtttt tttttacttt gataatatga atttttttta
     1021 ctgcttaaat tttcttcttg tattcctatt ggctttattg gtttaatagg gcttttgtac
     1081 ccttggggca tccttttgac agctagacga tatatgcgtt ttgcctgagg caagtcatta
     1141 tatcttttta accaatgtgt tagttccaca aaatccgatc gataacaaga aggatgcaaa
     1201 taacgctgag agagcacatg acccattaga atattattgc taatttctaa gatatatttg
     1261 tttgcttttt tccatttaca ttcttcttgc aaatcaaaaa tagcttgata ttttaataca
     1321 tcttcttctg acagaatttt ttcttcgaat atttttttat atttttttga tatatccaaa
     1381 tgtataactg atgcttgatt cgtaaaagaa ataaaaaaaa gagcaatcaa tatagtaata
     1441 agcttataaa ttttcatttt ctattttttt ctgtaattgc tgtaaatctt cccatgctgt
     1501 ttttttaaat cctggtgaac gcaacaaaaa agctggatga tatgttgcta gacaaagaat
     1561 gggttcaatc aaataaagac ttttgtattc atgccagcga cctctaagtt ttgaaatacc
     1621 aagaggggta gatagtacac tctttgccgc tatggctcct agaagaacaa taagttttgg
     1681 ctttattaat tcaatatgtt tttgtaggaa tgggaggcac tgtaatattt cttcatttga
     1741 aggagttcta ttatttggag ggcgccaagg aataacattg gtaatataaa ctttatttct
     1801 atctaaattt attgc
//
LOCUS       DTVV01000004            1031 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1016628, whole genome shotgun sequence.
ACCESSION   DTVV01000004 DTVV01000000
VERSION     DTVV01000004.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1016628"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(245..703)
                     /locus_tag="EYO86_00040"
     CDS             complement(245..703)
                     /locus_tag="EYO86_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41459.1"
                     /translation="MKEVEFITQDKQYELCYQDTLKVISEAKQKLYEVTKWIVTLQTL
                     VVGAAFSNKMDFSESLVVLPVFIGLMGVFLNKSISDELLVHRKTLANIRNEIGGVIYE
                     LNKDQVKHFLNGDKPKHVNYFFSYKISNFAIITLSSLVSICMVAFHKVAF"
BASE COUNT          367 a          182 c          123 g          359 t
ORIGIN      
        1 aatcaatcat gagatcgact tccttcttgg tcatggtttt gattgtaaag tgtttactct
       61 gcatctgctt tccttaaaaa gcttttttat cgtgaccgat aactttaagt tcaaagttaa
      121 ttttcttaat atttcacaaa gcaaattaag ctatttatat ttcgtatagt taaaataact
      181 aacattaccg ccaaataggt tcaacctaaa tctatttaaa taaatttaat ttgattaaga
      241 ttttttagaa ggcaacttta tgaaaagcaa ccatacatat agataccaag ctagataatg
      301 taattatggc aaaattacta atcttgtaag aaaagaaata atttacgtgt ttaggcttat
      361 ctccatttaa aaaatgttta acttgatcct tatttagttc atatataact ccaccaattt
      421 catttctaat gttagctagt gtttttctat gaacaagcaa ctcatctgat atagacttgt
      481 ttaagaatac ccccattaaa ccaatgaaca cgggtagtac tacaagcgac tcagaaaaat
      541 ccattttgtt agaaaacgca gcaccaacta ctaaagtttg cagagttaca atccatttag
      601 ttacttcgta aagcttttgc tttgcttctg aaatgacctt aagagtatct tgatagcata
      661 attcgtactg cttatcttgt gttataaatt ctacttcctt caatccatac ctccttaata
      721 atggaaataa gctttattct cagattgaac ctaagaccaa attcactaat gatgaacaaa
      781 ctacaaaact aaatttaatc gacttaaaga gcaactttaa ttaaaattat gtctcaaaaa
      841 caataagcta agaagaatgc aatttatcta tgtaccaaaa tagttgatat tcagttaaat
      901 cacatccagc cgacttattc atataacttt aagtagctaa agaaatttaa gttttatatt
      961 ttacttgctg ttatttcaat cctataaact tcaacaccct cagtaccaac ctcaatcgct
     1021 gcaggtttat c
//
LOCUS       DTVV01000005            2172 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1028217, whole genome shotgun sequence.
ACCESSION   DTVV01000005 DTVV01000000
VERSION     DTVV01000005.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1028217"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="EYO86_00045"
     CDS             complement(<1..454)
                     /locus_tag="EYO86_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41460.1"
                     /translation="MDKTTQFRGLNLCEYKKGEKHFVIIFYLKGQRHKPRRFPVGRFD
                     NNLNVITGETKFRVKQCQERLFKIAKEHQDEYGLWVKDPNLTIAFTSVNQSITIRDLI
                     KFNNNEVGRILIHDPFPFAIIKVVDSNINDFLYQDLICGSAKVEIAKPE"
     gene            complement(531..719)
                     /locus_tag="EYO86_00050"
     CDS             complement(531..719)
                     /locus_tag="EYO86_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41461.1"
                     /translation="MIKFNSSPEPTIGVEIELQIVDKNNLDLNNISSKVLADINKEFS
                     DKIKCELIESIIEIKIYR"
     gene            complement(720..1124)
                     /locus_tag="EYO86_00055"
     CDS             complement(720..1124)
                     /locus_tag="EYO86_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIC41462.1"
                     /translation="MLYFSYGSNLSHYQMNIRCFGSRYLKAFLLKNYKLIFCHPNKSN
                     KFGYANIIKKRGSKVAGAIWEITKEHEKILDNYEQFPNIYKKQYFYLGGKKIMFYIMK
                     KYIFKDPPKSYIDTINKGYEDCNIDLRINYRF"
     gene            complement(1126..1878)
                     /locus_tag="EYO86_00060"
     CDS             complement(1126..1878)
                     /locus_tag="EYO86_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIC41463.1"
                     /translation="MTKNIQVSSAIIIIGNEILSGRTKDINTSTIATWLNQLGVRVQE
                     ARVIPDVEAIIVSTINKLRKKYSYVFTTGGIGPTHDDITAQSISKAFGVKYGIHKEAY
                     KILEDYYKKSEFNEGRKKMAWMPVNAKLILNPTSGAPGFIMENVFCLPGVPSILQSML
                     GALNNEISGGKPIISHTINLRTVESEIAKSLTSVQDKNKDVEIGSYPFFRAGKLGVAI
                     VIRSIEQSKIDICNAQILDFVNQKNIQIIINE"
     gene            1902..>2172
                     /locus_tag="EYO86_00065"
     CDS             1902..>2172
                     /locus_tag="EYO86_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIC41464.1"
                     /translation="MKFSKRLIKGKLIKRYKRFFADINIKNEIITAHCPNTGSMMGLL
                     DQNNVVWISKNNDPKRKLKYTLEIIKAKDNLVGVNTHLANKIVYEG"
BASE COUNT          702 a          336 c          283 g          851 t
ORIGIN      
        1 attcaggttt tgctatttca actttagctg agccacagat caaatcttga tataaaaaat
       61 catttatatt tgaatcaaca actttgatta tggcaaaagg aaagggatca tgaattaata
      121 ttctacctac ttcattatta ttaaatttaa ttaaatctct tatggttatg gactggttga
      181 ccgaagtaaa agcaatagta aggttaggat cttttaccca caacccatat tcatcttgat
      241 gttcttttgc tattttaaat aacctctcct ggcattgctt aactctaaac ttagtttctc
      301 cagttataac atttaggttg ttatcaaatc tcccaactgg gaagcgtcta ggtttatgtc
      361 tttgaccctt aagatagaat attatgacaa agtgcttttc tcccttctta tattcacaaa
      421 ggttaagacc tctaaattgt gtggtcttat ccatttggaa ggaggtataa actctatcca
      481 gtggtttacc attcttactg atgaagttag acttatggtt atgtattgct ttatctgtaa
      541 attttaattt ctattattga ttctataagt tcacatttaa ttttatctga aaattcttta
      601 tttatgtcag caagtacttt tgaagatata ttgtttaaat ctaaattgtt cttgtctact
      661 atttgaagtt ctatctcaac ccctatagtt ggttctggac ttgagttaaa tttaatcatt
      721 taaaatctat agttaattct caaatcaata ttacagtctt cgtagccttt gttaattgtg
      781 tcgatataac tcttgggcgg atctttaaaa atatatttct tcataatata aaacataatc
      841 ttttttcctc ctaaatagaa atattgcttt ttataaatat tgggaaattg ctcataatta
      901 tctaatattt tttcatgttc ttttgtgatt tcccatatcg ccccagctac tttagatcct
      961 ctttttttta ttatatttgc atagccaaat ttatttgatt tatttggatg acaaaatatc
     1021 aatttgtaat ttttaagaag aaaagctttt aaatatcttg atccaaaaca tctaatattc
     1081 atttgataat gactaagatt acttccgtat gaaaaatata aaattttact cattaataat
     1141 tatttgaata tttttttgat ttacaaaatc taatatttgg gcattacata tatcaatctt
     1201 agattgctct atagatctta taacaatagc aactcctaat ttgcctgctc taaaaaaggg
     1261 gtaactacct atttcaacat ctttattttt atcttgaacg gaagttaatg attttgcaat
     1321 ttcactttca actgttctaa gattaattgt gtgacttata attggttttc ctccagaaat
     1381 ttcattattt aaagctccta acatcgattg taaaatggaa ggaactcctg gtaagcaaaa
     1441 tacattttcc ataataaaac ctggagcacc acttgttgga tttaatatca attttgcatt
     1501 aactggcatc caagccattt tttttcttcc ttcgttaaat tcactctttt tatagtaatc
     1561 ctctaatatc ttataagctt ctttatgtat tccatatttt acaccaaaag cttttgatat
     1621 tgactgagcc gttatatcgt catgtgttgg accaatcccg ccagttgtaa aaacatagct
     1681 atattttttt cttaatttat taattgtact tacaattatt gcttcaacgt ctggtattac
     1741 tcttgcttcc tgaactctca ctccaagttg gttaagccat gttgcaattg tacttgtgtt
     1801 tatatctttt gtccttccag atagaatttc attgccaatt ataataattg cagaactaac
     1861 ttgtatgttt ttagtcattt ataaatatat attattcaaa tatgaaattt agcaagagat
     1921 taataaaagg caaactcatc aaaagatata aaaggttttt tgctgatata aatataaaaa
     1981 acgagataat tactgctcac tgtcctaata ccggttccat gatgggtcta ttagatcaaa
     2041 ataatgtagt gtggatatca aaaaataatg atccaaaaag aaagttaaaa tacacattgg
     2101 aaataattaa agcaaaagat aatctagttg gtgttaacac gcatttagcg aataaaatag
     2161 tgtatgaagg tc
//
LOCUS       DTVV01000006            2396 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1029300, whole genome shotgun sequence.
ACCESSION   DTVV01000006 DTVV01000000
VERSION     DTVV01000006.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1029300"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            410..808
                     /locus_tag="EYO86_00070"
     CDS             410..808
                     /locus_tag="EYO86_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41465.1"
                     /translation="MRGLALFVIAISFLLSCPIFAAESFLFSGRIKCELSATFIVFDF
                     DKKIALRNNYISSRYRLSNYETKKSKFKKTVHTFSWYDVKDNFLGYWVISDDSRNVEE
                     IKFDREGKNFSVTRTCSEFNNGYIGELVWE"
     gene            861..1586
                     /locus_tag="EYO86_00075"
     CDS             861..1586
                     /locus_tag="EYO86_00075"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HIC41466.1"
                     /translation="MSNFSQIFVVGDIHGCHALLKQIHKKILDKLDNARGNKLLIYLG
                     DYIDRGPKIKETIQALLDFQPDNFQQVFLLGNHEQMMLDFINKVPDSFYLWILNGGDE
                     TLNSYEIKGAASVYDESKSNETVRNELVNSIPKDHLQFFNNLSLSYQWGKYFFVHAGI
                     DPGVSLDKQDKNTLIWQRGREFLFNTKSFEKVIVHGHTPQPEIEDLTNRINLDTGAFY
                     TGTLSCLIIETKTGKKQFINTTN"
     gene            complement(1834..2310)
                     /locus_tag="EYO86_00080"
     CDS             complement(1834..2310)
                     /locus_tag="EYO86_00080"
                     /inference="COORDINATES: protein motif:HMM:PF04519.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HIC41467.1"
                     /translation="MSFLHLKNKIEPQYRESKTSSIEEDAFGIKSKLGTSTVDNSKSS
                     LMIGEGVTITGTIKANSKVTIQGTVDGDIECNNITISKSGNVKGKIKTDTITVEGKAE
                     GEINAEDVLIIKSKGHVSGKVFYGEIQIEEGGKISGEINHRDKDSKQEEFKDLKVL"
BASE COUNT          738 a          447 c          457 g          754 t
ORIGIN      
        1 gaaaaagggg cggatttcat tctggaagac caaaaaaaca tgcctgctac ttaaaattta
       61 gtatgaaatt tttgtttatt ttaccctaag tcatgtgttt tttataatta tgcactgaaa
      121 aaataattaa ctggaaatta aagcttgtca taaatttaat tacttgtcag attgaacacg
      181 gtgattattt atggtgaagg ttaaagttaa aaatggtgac attactcaat ttcttcacgc
      241 ttgggacagg gaagcttacg atttaaattt ggagctaaaa aaaatgctct taaagcaaaa
      301 aaaggggcag gtttccccac ggaagaaaga agggcatact tttagagcgc attagcaatt
      361 gaagaattaa tttcttcctc tgaattatga aaggaatagt caagaagcta tgagaggctt
      421 ggccttgttc gttattgcaa tatcctttct tttgtcttgt ccgattttcg cggctgagag
      481 ttttcttttt tcaggcagaa taaagtgtga actgtctgcg acctttatcg tctttgactt
      541 tgataaaaaa attgcccttc gaaataacta tatttcttct cggtacagat tatcaaacta
      601 tgaaacaaaa aagagtaaat tcaaaaaaac tgttcatact ttttcctggt atgacgtgaa
      661 ggataatttt ttagggtact gggttatctc ggatgattca aggaacgtgg aagaaattaa
      721 atttgacagg gaagggaaaa atttttccgt aaccagaacc tgctcggaat ttaataacgg
      781 ttatattggc gaactggtgt gggaatagcc agcaaagacc ctgtccaatt ttgccgaccc
      841 attaaccaat gtattgaagg gtgagtaatt tttcccagat atttgttgtt ggcgacatac
      901 atggctgcca tgcattgctt aaacaaatac acaaaaaaat tctggacaag ttggataatg
      961 cacgcggcaa caaactactt atatatttgg gtgactacat tgacaggggg cccaagatta
     1021 aggaaacaat tcaagcgctt ctggactttc agcctgataa ttttcaacaa gtatttcttt
     1081 tgggcaatca tgaacagatg atgcttgatt tcatcaacaa agtaccggat agtttttatc
     1141 tttggatatt gaacggcggt gatgaaacat taaatagtta cgaaataaaa ggtgcagcct
     1201 ctgtttacga tgaaagcaaa agcaatgaaa cggtacgtaa tgaattagtc aacagcatcc
     1261 cgaaagacca tcttcaattt tttaacaatc tctccttatc ctatcaatgg ggcaagtatt
     1321 tctttgtcca tgccggcatt gaccctggcg tttccctgga taaacaggac aaaaacacat
     1381 tgatatggca acgaggaaga gaatttcttt ttaacacaaa aagctttgag aaagtcattg
     1441 tgcacggcca cacacctcaa cccgaaatag aagatttaac caaccgaata aacctggata
     1501 ccggggcatt ctacacagga accctctcct gcctaatcat tgaaaccaag acaggcaaaa
     1561 aacaatttat aaatacaaca aattaagaat caggctaaac acctttcctt aaaaatttaa
     1621 gaagattatt tttaagttaa cacatggttt tattataagc cttgataaag tagagccaga
     1681 agtctttaag catattggaa taacaccaag gctattccga tgagggaaat tactattaaa
     1741 tctataatca tctggaatca cagtagtgat caagcgtgtc caaaaagggc tggaaatgtg
     1801 gaatgattgt gatttgttga tgtcttgcct gcattacaaa accttcaaat ccttaaactc
     1861 ttcctgcttg ctgtctttat ctcggtggtt aatttccccg gatattttgc caccttcctc
     1921 aatctgaatt tcgccgtaaa aaacttttcc gctcacatgg cctttggatt taatgatcag
     1981 gacatcttcg gcatttatct cgccctccgc ctttccctca accgttattg tatcggtttt
     2041 aatttttcct tttacattgc ccgacttgct gatggtaatg ttgttgcact caatatcgcc
     2101 gtcaacagtg ccctggatgg ttactttgct gttcgccttg atcgttccgg tgatagtcac
     2161 gccttccccg atcatcaggg agctttttga attgtcaacc gtactcgtcc caagttttga
     2221 ttttatgccg aaagcatctt cctcgatgct tgacgttttt gattcgcggt attgtggttc
     2281 tattttgttt tttaaatgta aaaatgacat tgcacgtctc cttttgtttt ttttaatagc
     2341 aaatgacgac caaagaacgg cccgagtgtg ttcatgaaat ggtatttaca atttgc
//
LOCUS       DTVV01000007            3372 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1044315, whole genome shotgun sequence.
ACCESSION   DTVV01000007 DTVV01000000
VERSION     DTVV01000007.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1044315"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="EYO86_00085"
     CDS             complement(<1..1647)
                     /locus_tag="EYO86_00085"
                     /inference="COORDINATES: protein motif:HMM:PRK06299.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIC41468.1"
                     /translation="MQKNLVKKITNSEYEKLISNSLQQKESKEKSIVNGKIIAIENDM
                     AIVDVGLKSEGRIPLSEFTRPGQKAEIKLGEMLKVFIENVDGANGETKLSREKAIKQS
                     AWNYLQDCYNKNEVVTGIPFNRVKGGMSVDLDGVIAFLPGSQIDSRKIIKDTKELLNK
                     PLDLMILKMDKFRGNIVVSRKAISDIELKEQRQALLSNISEGSVISGKIKNITDYGVF
                     VDLGGIDGLLHVTDISWKKISHPSDVLELGAQIDVKILKFDEELTRLSLGIKQLSDDP
                     WLKVNDLYSVDQKIKSPVSSISDQGVSIIVDENYDGFIPLGELTWLKKPPHPSKVVEL
                     NQELEVKITEIDNEKRRLICSLKNLKENPWKGLTKKYSINSNIDTEVVNVVDFGIFVK
                     VHEEIDGMVHVSDLSWNEETCSTILSDIKKGDIVKVKILDINIEKERISLGIKQLEID
                     PVQKFINDYPLKSRISGKITAIDDKGLTINLGKNVNGYIKKTNLSKDKIDQKVERFAI
                     GEMVDSMIVSIDNKIRNINLSIKEIEIDDEKKTLSKYGSSD"
     gene            complement(1649..2290)
                     /locus_tag="EYO86_00090"
     CDS             complement(1649..2290)
                     /locus_tag="EYO86_00090"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:PRK00023.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIC41469.1"
                     /translation="MNNFIIAVDGPAGSGKQRISKYIAKKYNLFHLDSGVLYRRMAST
                     ILKQKIDYNNQFNIKRLIDEIIYLSPRNHKSLRTENIGNITSKIAITPLVRKFVNNQQ
                     KIIVKEMLKLYIGCVIDGRDIGSKVFRDAKIKLFIEVPAEIRAKRRHKQLIALGEKSI
                     YGQILKEINLRDKTDKRRKESPLIKPARAIIIDNSNNFQKTIIQIKKALIEIK"
     gene            complement(2283..>3372)
                     /gene="aroA"
                     /locus_tag="EYO86_00095"
     CDS             complement(2283..>3372)
                     /gene="aroA"
                     /locus_tag="EYO86_00095"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIC41470.1"
                     /translation="GNGLNSLKKPLKDIYLGNSGTGARLLTGLLSAQNFKSALIGDES
                     LSSRPIKRITTPIELMGGKFEYKNGTLPLHIFGKELKPISYKIPIPSAQVKSGIILAA
                     LNTKGQTTINETSITRDHTEIMLKSFGANIKIKKESDMNSIFINGKQELTSKNIYVPC
                     DLSSSSFFIVAAMINKNSNLKLQNININPTRDGILHALKLMGGNITISNQRLINDEII
                     GDIEVQSSHLKGCELNEDMAKLMIDEYPILSVAASFAKTPSLFKGLKELKVKESDRLE
                     LIRFNLNQCGITCEVLNDNLFIDPRKKNKIKNNKIKTSFDHRIAMAFAIMGSKLDHDL
                     QISNSNCINTSFPNFIKSFNKIGGNLIE"
BASE COUNT         1030 a          543 c          424 g         1375 t
ORIGIN      
        1 atcactagag ccatatttag aaagagtttt tttctcatca tcaatttcaa tttctttaat
       61 tgatagatta atattacgta ttttgttatc aattgataca atcatagaat ctaccatttc
      121 tccaatagca aatctctcta ctttttgatc tatcttatcc ttagataaat ttgttttttt
      181 aatataacca ttcacattct tacctagatt aattgttaaa cccttatcat ctattgctgt
      241 aatttttcct gaaattcttg atttcaaggg gtaatcatta ataaattttt gaacaggatc
      301 gatttctaac tgtttaatac ctagactaat cctttctttt tcaatattga tatctaatat
      361 tttaactttt acaatatcac cttttttaat atcagatagt attgttgagc aagtttcttc
      421 attccaacta agatctgata catgaaccat accatctatt tcttcatgaa ccttaacaaa
      481 aataccaaag tcaacaacat tgaccacttc tgtatcaata ttactattta tactgtattt
      541 ttttgttagt cctttccaag ggttttcttt gagatttttt aagctacaga ttaaccttct
      601 tttttcattg tcaatttcag taattttaac ttcaagttct tgatttagct cgactacttt
      661 cgagggatgt ggtggttttt ttaaccaggt taattcaccg agaggtatga agccatcata
      721 attttcatca actataattg atacaccttg atcactgata gaagaaactg gagatttgat
      781 tttttgatca acagaatata agtcatttac tttaagccaa ggatcatcag atagttgttt
      841 aattcccaag cttaaccttg ttaattcttc atcaaatttt aaaattttta catcgatttg
      901 agctcctaac tctaaaacat cggatggatg actaattttt ttccaagata tatcagtaac
      961 atgtaaaaga ccatcaatac cccctagatc aacaaataca ccataatcag taatattttt
     1021 tatttttcct gaaattacag atccttctga tatgttggac aacaatgctt gtctttgctc
     1081 ttttaattca atatcagaaa tagcttttcg agacacaacg atattgcctc tgaatttatc
     1141 catttttaaa atcattaaat caagaggttt attaagaagt tctttggtgt ctttaattat
     1201 ttttcttgag tctatttgac taccaggtaa aaaggctatt accccatcta gatcaacgct
     1261 cattccacct tttacacgat taaaaggaat ccctgttact acttcatttt tattataaca
     1321 atcttgcaaa tagttccaag cactttgttt gattgctttt tctcttgaaa gttttgtttc
     1381 accatttgct ccatctacat tttcaataaa aacctttaac atttctccaa gtttaatttc
     1441 tgctttttgt cctggtctgg tgaattccga taaaggaatt ctaccttcgc ttttaagtcc
     1501 tacgtcaact attgccatgt cattttctat agcaatgatt tttccgttaa caatagattt
     1561 ttctttactt tctttttgtt ggagtgaatt tgatattaat ttttcgtatt cagagttggt
     1621 gattttttta actaaatttt tttgcatatt atttaatttc gataagtgct tttttaattt
     1681 gtattatagt tttttgaaaa ttattcgagt tatctataat tattgctcta gcaggcttta
     1741 ttaatggcga ctcttttcgt cttttatcag ttttatctct caaattaatt tccttcaaaa
     1801 tctggccgta tatagacttt tcgcctagtg caatcaactg tttatgtctt ctttttgctc
     1861 taatttctgc tggtacttcg ataaaaagct ttattttagc atctctaaaa acttttgaac
     1921 caatatctct tccatctata acacaaccaa tatatagttt taacatttcc tttacgatta
     1981 ttttttgctg gttattaaca aattttcgaa caagtggagt gatggcaatt tttgaagtta
     2041 tattacctat attttctgtt ctaagtgatt tatgattgcg aggtgacaaa taaataatct
     2101 catcaattaa tctttttata ttaaattgat tattgtaatc aattttttgt tttaaaatag
     2161 tagaagccat tcgacgatat aaaactcctg aatcaagatg aaataaatta tatttttttg
     2221 caatatattt tgatatacgt tgcttgcctg aaccagcagg gccatcgact gcaataataa
     2281 aattattcaa ttagattccc accaatttta ttgaaacttt ttataaaatt aggaaaacta
     2341 gtattaatac aatttgagtt acttatttgt aaatcgtggt ccaacttgct tcccataatt
     2401 gcaaaagcca ttgcaattct atgatcgaag cttgttttga ttttattatt tttaatttta
     2461 ttttttttac gcggatcaat aaaaagatta tcatttaaaa cttcacatgt aattccacat
     2521 tgattgagat taaatcttat taattctagt ctatcacttt ctttgacctt taattctttg
     2581 agacctttga ataatgaggg agttttggcg aatgaggcgg caacagaaag aataggatat
     2641 tcatcaatca ttaattttgc catatcctca ttcaattcac atcctttaag atggcttgat
     2701 tgaacttcta tatcacctat gatttcatca ttgattaatc tttgattaga aattgttata
     2761 tttcccccca ttaattttaa tgcatgcaga ataccatctc tagttggatt aatattaata
     2821 ttttgaagtt ttaaatttga gtttttattt atcatggcag caacaatgaa aaaagaacta
     2881 ctcgataaat cacaaggaac gtaaatattt ttagaagtta attcttgttt tccatttata
     2941 aaaattgaat tcatatcact ttcttttttt atcttaatat ttgcaccaaa ggattttaac
     3001 attatttctg tatgatctct tgtaatgcta gtttcattta tagtagtttg accttttgta
     3061 tttaatgcag caagaattat tcctgattta acttgagcag aagggattgg aattttatat
     3121 gaaattggtt ttaattcttt tccaaagata tgaaggggta aagtgccgtt tttgtactca
     3181 aatttacctc ccattaattc aattggagtt gtaattcttt ttataggtcg tgatgaaaga
     3241 gattcatcac caatgagtgc acttttaaaa ttttgtgcag ataaaagacc tgttaatagt
     3301 cttgcacctg tacccgagtt gccaagataa atatctttca aaggtttttt tagagaattt
     3361 aaaccatttc ca
//
LOCUS       DTVV01000008            2573 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1046674, whole genome shotgun sequence.
ACCESSION   DTVV01000008 DTVV01000000
VERSION     DTVV01000008.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1046674"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="EYO86_00100"
     CDS             complement(<1..1287)
                     /locus_tag="EYO86_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC41471.1"
                     /translation="MANTAEETKVNNQIIENLFNKCEASIKAFDNLIEKAKNLTKKKI
                     VKDGSIDNALLEKEQFLCHGFSWLASYNFALREILNWAKNLEKNKKSFDLEKLILQSA
                     FGEYLSQIIGGISMCQTEIIRAGDFGLTDEDINAFLIDEVKGLIKYGNTNDVRMQIAK
                     HIVDNNYGNVGLEDETLEMIKDQFKKFSEDQVLPYAHEWHLKDELIPMEIIKQMADLG
                     VFGLTIPEEFGGLGMKKIAMCVVTEELSRGYIGIGSIGTRAEIAAELIKLGGRDEQKK
                     KWLPKIASGEILPTAVFSEPNTGSDLGSLKTRAVKEGNVYKITGNKTWITHGARTDIM
                     TMMVRTNPNEKGYKGLSIILAEKPRGTDKEPFPAKGMSGSEIEVLGYRGMKEYEIAFD
                     NFETKSENLLGSEEGKGFKQLMETVESARIQTAARAI"
     gene            complement(1306..2535)
                     /gene="ccrA"
                     /locus_tag="EYO86_00105"
     CDS             complement(1306..2535)
                     /gene="ccrA"
                     /locus_tag="EYO86_00105"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HIC41472.1"
                     /translation="MQDKKDLYEIGEIPPLGHIPKQMYAWLIRQERHGEPRDAMKIEI
                     VDAPKIGENDVLVMVMAAGVNYNGVWASLGKPVSPFDYHKADFHIAGSDASGIIWAVG
                     PNVKRWKVGDEVVIHCNQVDGDDEECNGGDPMLSSSQKIWGYETPDGSFAQFTAVQQQ
                     QIMHRPKHLTWEESGCYVLCLATAYRMLYGHQPHTLKPGQNVLVWGASGGLGVFATQL
                     IKHAGANAIGVVSSDDKINYVKKLGAVGAINRKNFQCWGELPNVDNIKAYTEYSDKVK
                     EFGKAIWEFTGKGISVDTVFEHPGGQTFPVSCFVVKRGGMVVYCAATSGYNLTFDARY
                     GWMHQKRIQGSHFATLKQAYEANNLVINKKIQPCMSEVLMWDKIPEAHMKIRENKHKP
                     GNIAVLVQAKKAGLKTV"
BASE COUNT          738 a          534 c          311 g          990 t
ORIGIN      
        1 gatagctcgc gctgcagttt gaattcttgc agattcaaca gtttccatta attgtttaaa
       61 acctttacct tcctcactac ctaacaaatt ttctgatttt gtttcaaaat tatcgaaagc
      121 aatttcatat tctttcatac ccctatatcc taaaacttca atttcacttc cgctcattcc
      181 ttttgctgga aaaggttctt tatctgtacc tcttggtttt tctgctaaaa ttatacttaa
      241 ccccttgtat cccttttcat taggattagt tctaaccatc attgtcatta tatctgttct
      301 tgctccatga gtaatccatg ttttatttcc agtaatttta taaacgtttc cttctttaac
      361 agctctagtc tttaaacttc ctaaatcaga tccggtattg ggttcactaa acacagcagt
      421 tggcaaaatt tcacccgagg ctatctttgg taaccatttt ttcttttgtt catctcttcc
      481 acctaatttt atcaattctg ctgctatttc tgcacgggtg ccaattgaac ctattcctat
      541 atatccacga cttaactctt ctgtaacaac gcacattgct atttttttca ttccaaggcc
      601 accaaattct tctggtattg ttagtccaaa aactcctaaa tctgccattt gtttaattat
      661 ttccataggt attaattcat cttttaaatg ccattcatgt gcataaggta aaacttgatc
      721 ttctgaaaat tttttaaatt gatcttttat catttcaagc gtttcatctt ctaacccaac
      781 atttccataa ttattatcta caatatgttt agcaatttgc attctaacgt cgtttgtatt
      841 tccatatttt ataagacctt ttacctcatc tattaaaaaa gcatttatat cttcatctgt
      901 taggccaaaa tcacctgctc tgattatttc agtttgacac attgatatac cgccaatgat
      961 ttgggataaa tattcaccaa aggcagattg taaaattaat ttttcaagat cgaaactttt
     1021 tttatttttt tcaagatttt ttgcccagtt cagaatttct cttagagcaa aattatatga
     1081 tgctaaccag gaaaatccat gacaaagaaa ttgctctttt tctaaaagcg cattatcgat
     1141 agaaccatct ttaacaatct tttttttcgt caagtttttt gctttttcta ttaaattgtc
     1201 aaaagctttt atcgatgctt cacatttatt aaaaagattt tcaattattt gattattaac
     1261 ttttgtttct tcagcggtat tggccattgt attaataata tattgttata cagttttcaa
     1321 accagctttc tttgcttgaa ctagcacagc tatatttcca ggtttatgtt tattttctct
     1381 aattttcata tgagcttctg gtattttatc ccacattaat acttccgaca tacatggttg
     1441 aatttttttg ttaattacta aattatttgc ttcataagct tgttttaatg tagcaaaatg
     1501 actaccttga attctttttt gatgcatcca tccatatctg gcatcaaatg ttaaattgta
     1561 acctgatgtt gctgcacaat aaacgaccat tccccctctt tttacaacaa aacatgaaac
     1621 aggaaaagtt tgtccgccag gatgttcaaa aactgtatca accgaaatac cttttccagt
     1681 aaattcccaa atcgcttttc caaactcttt aactttatct gaatattctg tataagcttt
     1741 aatattatct acatttggta actcccccca acattgaaaa ttttttctat tgattgcacc
     1801 tactgcgcca agttttttta cataattaat tttatcatca cttgatacaa cccctattgc
     1861 atttgcgcct gcatgtttta ttaattgtgt agcaaaaaca cctaaacctc cgctagctcc
     1921 ccataccaaa acattttgac ctggttttaa agtatgcggt tgatgtccat aaagcattct
     1981 atatgcagta gctaaacaaa gaacataaca tccactttct tcccatgtta agtgtttagg
     2041 tctgtgcatt atttgttgtt gttgcactgc agtaaattgg gcaaaagatc catcaggtgt
     2101 ttcatatccc caaatttttt gagaactaga gagcataggg tcgccaccat tacattcttc
     2161 atcatctcca tcaacttgat tacaatgaat tacaacttca tctcccactt tccatctttt
     2221 aacatttgga ccaactgccc aaataattcc tgatgcatct gaaccggcta tatgaaaatc
     2281 tgctttatga taatcaaatg gtgaaactgg ttttcctaaa gatgcccata ccccattgta
     2341 atttactcct gctgccataa ccattactaa gacatcattt tctccaattt tcggagcatc
     2401 aacgatttca attttcattg cgtctcgagg ctcaccatga cgttcctgtc gaattaacca
     2461 agcatacatt tgtttaggta tatggcctaa tggaggtatt tcccctattt catataaatc
     2521 ttttttatcc tgcatttttt tctttaatat aataaattaa atataaagcc taa
//
LOCUS       DTVV01000009            3757 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1051142, whole genome shotgun sequence.
ACCESSION   DTVV01000009 DTVV01000000
VERSION     DTVV01000009.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1051142"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="EYO86_00110"
     CDS             <1..706
                     /locus_tag="EYO86_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41473.1"
                     /translation="FVITDPAYIRSLKFTLFYIFVITLVTGSIGLLLAVQANSVIHGK
                     SAYKTLLIWPYAIAPAVVGVSANYFFSERLGLLYGFFNDLWGFNWMENVSDAYIYVIF
                     AGCWKQISANFIFFLAGLQNIEKSVIEASSIDCKSSFRRFWTVTFPLLMPTTFFLIII
                     NITYAFFETLGIIDTTTIGAPSGETKTLVYKAYIDGFKGLDLGSAGAQSIIMMIIVLV
                     ITIFQFRFIEGKVHYN"
     gene            706..1533
                     /locus_tag="EYO86_00115"
     CDS             706..1533
                     /locus_tag="EYO86_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41474.1"
                     /translation="MESKYSQFSTHLILILGILIMILPIWIIFASSTHDNLTLATKGM
                     QFGFGDNFLSNYHNVVFTKGGFSKEITLLNLFLNSFIMAIGIATLTVVTSLLAAYTIV
                     YFKIKLASTFFWLIFITLLVPLEVRILPSYQVVSDLNLINTYAGLILPLLASSIATFF
                     FRQFFKTIPEELLEAAKIDGVNSWKFFIDFLIPLSKTMMVAIFIFMFVYGYNQYLWPI
                     IVTTNEDFWTVIMGMKIIFMRSLVSSFSYVILSIIPPILVVIFLQRWFVKGLINSGK"
     gene            1530..2381
                     /locus_tag="EYO86_00120"
     CDS             1530..2381
                     /locus_tag="EYO86_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41475.1"
                     /translation="MNNKFNQFFTHTILFCGVLLMVIPIWIAFASSTHDNITILTEGM
                     KWSLGDQLLKNYNEVLNQTGGFSKEITATKMFINSFIMALGIAIVKVIISAMSAYAIV
                     YFRFKWATPLFWLIFMTLLVPLEVRILPSYQIVSDLNLTNTYTGLILPLVASATATFF
                     FRQFYKSIPDELLESAKLDGANSWKFFIDFLVPLSRTMIAAIFIFMFVYGYSQYLWPL
                     IMTTSEQYWTVVMGMKIIFMESYEGGNLPRVAERFAYIILSIIPPVLIIIFLQRWFIK
                     GLIQTEK"
     gene            2382..3434
                     /gene="ugpC"
                     /locus_tag="EYO86_00125"
     CDS             2382..3434
                     /gene="ugpC"
                     /locus_tag="EYO86_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HIC41476.1"
                     /translation="MSFLEISNVTKIYPNGTQAVHETSLSIEKGEFMVFVGPSGCGKS
                     TLLRMVAGLEDISQGEISLDGKVINKIDPSERDVAMVFQNYALYPHMNVYNNLAYGLK
                     NRGIDKTLIREKVIETAELLQISQFLDSKPSLLSGGQRQRVAMGRAIVRNPKIFLFDE
                     PLSNLDALLRIKMRLEIKKLQREMGVTSIFVTHDQVEAMTLADRLAVINNGIIEQLGT
                     PIEIYNNPQSIFVAGFIGSPQMNFLDAELKNNVLKTASFTIPNFISNFNGEVILGIRP
                     EHFLPSNKGEINISVDLVERLGSDNLIYGEMNGKKDFCYRSPGNVSVNIGDKLTLNIV
                     DNKFYVFDKITNKRIN"
     gene            3576..>3757
                     /locus_tag="EYO86_00130"
     CDS             3576..>3757
                     /locus_tag="EYO86_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41477.1"
                     /translation="MDKYIIENEEIFIKGELKGYPLKEAENKFDHNGKPLAFPGCSII
                     SKIPIKSDLFYEIKLL"
BASE COUNT         1264 a          489 c          635 g         1369 t
ORIGIN      
        1 atttgtaatt acagaccctg cttatattag gtcattaaaa tttacattat tttatatctt
       61 tgtaataaca cttgtgactg gatccatagg attacttctg gccgttcaag caaatagcgt
      121 tatacatggg aaaagtgctt acaaaacatt actgatctgg ccttacgcta ttgctcctgc
      181 agtagtggga gtttcagcaa attatttttt tagcgaaagg ttaggtttac tttacggttt
      241 ttttaatgat ctttggggat ttaattggat ggaaaatgtt tcagatgcat acatttatgt
      301 tatttttgca ggttgttgga aacagataag cgcaaatttt attttctttt tagcagggtt
      361 acaaaatatc gaaaagtctg taatagaagc ttcgtcgatt gactgcaaaa gtagttttag
      421 aagattttgg acagtaactt ttccattact gatgccaact actttctttt taattataat
      481 aaatattact tatgcttttt ttgaaacgtt gggcataatt gatactacta ctattggagc
      541 cccaagtggg gaaaccaaga ctttagttta caaagcttat attgatggat ttaaaggtct
      601 tgatttaggt agtgcaggtg cacaatcaat aataatgatg ataatagttt tagttataac
      661 aatatttcaa ttcagattta tcgaaggaaa agtacattat aattaatgga atcaaaatac
      721 tcacaattct ctactcactt aattttgatc ttaggtatat taatcatgat tttacctatt
      781 tggattattt ttgctagctc tactcatgat aatttaacac ttgctacaaa aggaatgcaa
      841 tttggctttg gagataattt tttaagtaat taccataatg tagtatttac aaagggaggg
      901 ttttcaaaag aaataacttt attaaactta tttttgaata gttttataat ggctattgga
      961 attgcaactt tgacagtcgt tacatcttta ttggccgcat ataccattgt atactttaaa
     1021 atcaaactgg cttcaacatt tttttggtta atatttataa ctcttcttgt gccgcttgaa
     1081 gtaagaatac ttccaagtta tcaggtagtt tcagatctca atttaatcaa tacttacgcg
     1141 ggattaattc tacctttgtt ggcttcatca atagctactt tttttttcag acaattcttt
     1201 aagaccattc ctgaagaatt attagaggca gctaaaattg atggtgtaaa tagctggaaa
     1261 ttttttattg attttttaat tccactttca aaaacaatga tggtagctat tttcattttt
     1321 atgtttgtat acggttataa ccaatattta tggcccataa ttgtcacaac taatgaagat
     1381 ttttggactg taattatggg aatgaaaatt atttttatga gaagtttagt aagtagtttt
     1441 agttatgtaa tattgtcaat tatcccgccg atactagttg taatattttt gcagcgttgg
     1501 tttgtcaaag ggttaataaa ttcaggaaaa tgaataataa atttaatcaa tttttcacgc
     1561 acactattct tttttgtgga gtgttactaa tggttattcc tatctggata gcttttgcca
     1621 gctctactca tgacaatatt actattctaa ccgagggaat gaaatggagt ttgggtgatc
     1681 aattgttaaa aaattataac gaagttttga atcaaactgg aggattctct aaagagatta
     1741 ctgcaacaaa aatgtttatt aacagtttta taatggcttt agggattgca atagttaaag
     1801 tgataatttc agcgatgtct gcttatgcga tagtttattt tagatttaag tgggcaactc
     1861 cccttttttg gttaatattt atgacccttc ttgtaccttt agaagttagg attttgccga
     1921 gttatcaaat agtttctgat ttaaatttaa cgaataccta tactgggtta attttgccac
     1981 tagtagcttc agccactgca acttttttct ttagacaatt ttataaatct attccagatg
     2041 aattattgga atctgctaaa ttggatggtg caaatagttg gaagtttttt atagattttt
     2101 tagttccact ttcaaggact atgattgcag caatatttat atttatgttt gtttatggtt
     2161 atagtcaata tttgtggcca ttaataatga caacatccga gcaatattgg acggtagtta
     2221 tggggatgaa aattatattt atggaaagtt atgaaggtgg gaatcttcca cgagtagctg
     2281 aaagattcgc ttatattata ttgtcaatta taccacctgt tttaataatt attttcttac
     2341 aaagatggtt tattaagggt ttaatccaaa cagagaaata aatgagtttt ttagaaataa
     2401 gtaacgttac aaaaatttat cccaacggaa cccaagcggt tcatgaaaca tccttaagta
     2461 ttgaaaaagg agaatttatg gtctttgttg ggccaagtgg ttgtggtaaa tcaacattac
     2521 taagaatggt tgctggttta gaagatatta gccaaggaga aatcagtcta gatggtaaag
     2581 tcattaataa aattgatcct tctgaacgtg atgtagcaat ggtatttcag aattatgcct
     2641 tataccctca tatgaatgtt tataataatt tagcttatgg attaaaaaat agaggaatag
     2701 acaaaacatt aattagagaa aaagtaattg aaactgcaga actattgcag atttcacaat
     2761 ttttagattc caaaccttct ttactttcag gaggtcagag acaaagagtg gcaatgggga
     2821 gggcaattgt aaggaatcct aaaattttct tatttgatga accattatct aatttagatg
     2881 cattacttag aattaaaatg cggttagaaa taaagaaact ccaaagagaa atgggtgtaa
     2941 caagtatttt tgtaacccat gatcaagttg aggctatgac tttagctgat agactagcag
     3001 ttattaacaa tggtattata gaacaattag gaactccaat tgagatatac aataatccac
     3061 aatctatatt tgtggctgga tttataggtt ccccacaaat gaacttttta gatgctgaat
     3121 taaaaaataa tgtattaaaa acagctagtt ttacaattcc caatttcatt agcaatttta
     3181 atggtgaagt tatactaggt ataagacccg agcatttttt accttccaat aaaggggaaa
     3241 taaatatatc cgtagattta gttgagcgat tgggatcaga taatcttatt tatggtgaaa
     3301 tgaatgggaa aaaagatttt tgttatcgtt cacctggtaa tgtttctgtc aatatcggag
     3361 ataaattaac tttaaacatt gttgataaca aattttatgt tttcgataaa ataactaaca
     3421 aaagaataaa ttaacattta ttctttttaa aacaaaccta ctaaaacata ttttcactta
     3481 gtatcatagt atcaaggatt gttctttaaa aaatactcta gtaactccta aatattttct
     3541 gttaaattaa ttcaattgat taatatgctt ttaaattgga taaatatata attgagaatg
     3601 aagagatatt tattaagggg gaattaaaag ggtacccact taaagaagca gagaataaat
     3661 ttgatcataa tggaaaacct ctagcctttc caggatgttc tataattagt aaaatcccaa
     3721 taaagtctga cttattttac gagataaaat tattaca
//
LOCUS       DTVV01000010            1417 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1051312, whole genome shotgun sequence.
ACCESSION   DTVV01000010 DTVV01000000
VERSION     DTVV01000010.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1051312"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="EYO86_00135"
     CDS             <1..326
                     /locus_tag="EYO86_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIC41478.1"
                     /translation="KEKCFLEVKNVTLLRENKIAEFPDAITSRGTKHLNELCNAKKKG
                     YQSYILYLIQRENCNSFKIAKDIDEEYKIAFSKALKSGVKILCYDCKLNSEEIKLNKQ
                     INYEQ"
     gene            316..1092
                     /gene="map"
                     /locus_tag="EYO86_00140"
     CDS             316..1092
                     /gene="map"
                     /locus_tag="EYO86_00140"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIC41479.1"
                     /translation="MSSNYKESFEQIKIAGNLAADTLDEVTSYVKPGVTTSKIDKICY
                     EFIKDNGGYSAPLFYRGFPKSCCTSVNHIVCHGIPTNKYLKEGDIVNVDVTAIVNGWH
                     GDTSRMFFVGDVSVKSKNLVSTTYNSMMRAIAIIKSGVNLGDIGEAIQAYVEKKGFSV
                     VRDYCGHCIGKTFHEPPNILHYGKKGEGVKLETGMIFTVEPMINEGIYNTKLLNDGWT
                     AVTKDKSLSAQFEHTIGVAKDGFEIFTKSKKKYEQPPYLI"
     gene            1089..>1417
                     /locus_tag="EYO86_00145"
     CDS             1089..>1417
                     /locus_tag="EYO86_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIC41480.1"
                     /translation="MIERYSREEIKKIWGEKNKYQIWLNIEIAAAQAMEKLKIIPKGV
                     SSKTRRKAKINVSRIHKIENKVHHDVIAFLTSITEKIGAEGKYLHKGMTSSDILDTCF
                     NIQLMQAG"
BASE COUNT          562 a          170 c          256 g          429 t
ORIGIN      
        1 ataaagaaaa atgctttctt gaagtaaaaa atgttacttt gctaagagaa aacaaaatag
       61 ctgaatttcc tgacgccatt acctcaagag gtacaaaaca tttaaatgaa ctttgtaatg
      121 ctaaaaaaaa agggtatcaa agttatattt tgtatttaat tcaaagagag aattgtaatt
      181 cttttaaaat tgctaaagat attgatgaag agtataaaat tgcttttagt aaagctttaa
      241 aaagcggagt taaaatcctt tgttacgatt gtaaattgaa tagtgaagag ataaaactta
      301 ataaacaaat taattatgag cagtaattat aaagaatctt ttgaacaaat aaaaattgca
      361 ggcaatttag ctgctgatac actggatgag gttacttctt atgtaaaacc tggtgtgaca
      421 actagcaaaa tagataaaat ttgttatgaa tttataaaag ataatggagg atattccgct
      481 ccactttttt atagaggttt tccaaaatct tgttgcactt cagtcaatca tattgtgtgt
      541 cacggaattc caactaacaa atatttaaaa gaaggagata tagttaacgt tgatgttaca
      601 gcaatcgtta atggttggca tggtgataca agcagaatgt tttttgttgg agatgtatca
      661 gtaaaaagca aaaatttagt ttcaacaaca tacaattcta tgatgagagc tattgccata
      721 attaaaagtg gggtaaattt gggagatatt ggtgaagcta ttcaagcgta tgttgaaaaa
      781 aagggttttt ctgttgttag agattattgt ggccattgta taggcaaaac atttcatgag
      841 cctccaaata tattacacta tggaaaaaaa ggtgaaggag ttaaattaga aacaggaatg
      901 atatttactg ttgaaccaat gattaacgaa ggcatatata atactaaatt acttaacgac
      961 ggttggactg cagtaacaaa agacaaatca ttatcagcac aatttgaaca tactattggc
     1021 gtagcaaaag atggatttga aatttttaca aaatccaaaa aaaaatatga gcaacctcct
     1081 tatttaatat gatcgaaaga tattcaagag aagagattaa aaaaatttgg ggagaaaaaa
     1141 ataagtatca aatatggtta aatatagaaa tagctgccgc tcaggctatg gaaaagctta
     1201 aaataatccc taagggtgta tcttctaaaa ctcgaaggaa agcaaagatc aacgtaagta
     1261 gaatacataa aattgaaaat aaggttcatc atgacgtaat cgcattttta acttctatta
     1321 ctgaaaaaat cggtgctgaa ggaaaatatt tgcataaagg aatgacgtcc tctgacattt
     1381 tagatacttg tttcaatatt cagttaatgc aagcggg
//
LOCUS       DTVV01000011            1563 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1051431, whole genome shotgun sequence.
ACCESSION   DTVV01000011 DTVV01000000
VERSION     DTVV01000011.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1051431"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="EYO86_00150"
     CDS             complement(<1..899)
                     /locus_tag="EYO86_00150"
                     /inference="COORDINATES: protein motif:HMM:PF13623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41481.1"
                     /translation="MFRSLGKSKIAFVLAILFGISLFFFKGGSRYSNLFNSDTVVATV
                     SGTPISTSKFNRTMKININKFNQMLGKPITGDEIRSFQIHSLSLEALINDAVFENEYD
                     SIKLIIDESVVAQKTKERIPQLYDKNNKLNETYLNTFLKQQQLKIEDIVQIINFETRD
                     EFVNNALFNINYPQYFSSKIDNYNNHSRKISYIEFLLEEVNIENDIQKYTLNMKEELE
                     KYYENNINQYMSKENRNVEYIIFNKKKYANNFVPTNFEIKEYFNANKELYYQKEKRSF
                     TQFNFKKLEEAKNFKSKIQNFINP"
     gene            841..>1563
                     /locus_tag="EYO86_00155"
     CDS             841..>1563
                     /locus_tag="EYO86_00155"
                     /inference="COORDINATES: protein motif:HMM:PF00121.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triosephosphate isomerase"
                     /protein_id="HIC41482.1"
                     /translation="MPNNIASTNAILDFPRLLNIFLFYGVACIIILSIRESKNMLEIN
                     KFDRIIVANWKLNGSIHFIEQYLQELNCSKLENSNNCGIICTPSVYFHKLVGRLNPLF
                     LGGQDCSNYSEGAYTGEVSATMLKDASCQFCIIGHSERRQIFGQSNEDVCIKAMNLIA
                     NNINPIICIGETFEEKNQNLTKDIIYTQISKSVPKNATKDFVIIAYEPIWAIGTGKIP
                     SLEEIDNIHSFIKNEIQNIENYK"
BASE COUNT          542 a          191 c          210 g          620 t
ORIGIN      
        1 gggtttataa aattttgtat tttagactta aaattttttg cttcttctag ttttttaaaa
       61 ttaaattgag taaaacttct tttttctttt tgataatata attctttatt tgcattaaaa
      121 tattctttaa tttcaaagtt tgttgggaca aaattattag catatttttt tttgttaaat
      181 attatatact ctacatttct attttctttg gacatatatt gattaatatt gttttcataa
      241 tatttttcta attcttcttt catatttagt gtatactttt gaatatcatt ttcaatattt
      301 acctcttcta gaagaaattc aatataggaa atttttcttg aatgattatt ataattatca
      361 attttacttg aaaaatattg aggataatta atattaaaca aggcattatt tacaaattca
      421 tctcttgtct caaaattaat tatctgcaca atgtcttcaa tctttaattg ctgttgtttc
      481 aagaatgtat tcaaataagt ttcatttaac ttattatttt tgtcatatag ttgtggtatc
      541 ctttctttag ttttttgagc aacaacggat tcatcaatta ttaatttaat actatcatat
      601 tcgttttcaa aaaccgcatc attaatcaaa gcttccaaag aaagtgaatg tatttgaaaa
      661 gatctaattt catcccctgt tataggtttg cctaacattt gattaaattt atttatattt
      721 attttcatag ttctgttaaa cttagatgtt gagattggcg ttcctgaaac tgttgcgaca
      781 accgtatctg aattgaataa atttgagtaa cgtgagcccc ctttaaaaaa gaataaactg
      841 atgccaaata atatagcaag cacaaacgca attttagatt ttcctagact tctaaacata
      901 tttctttttt acggcgttgc ttgtataata atactttcta taagggaaag taaaaatatg
      961 ctcgaaatta acaaatttga tagaataatt gtagcaaatt ggaaattaaa tggttcaatt
     1021 cattttattg aacaatattt gcaggaactt aactgtagca aattagaaaa ctctaataat
     1081 tgtggaatta tatgtacacc atcagtatat tttcacaagt tagttggtag attaaaccct
     1141 ctttttttgg gtggacaaga ttgttctaat tatagtgagg gcgcttatac aggagaggta
     1201 agtgcgacaa tgcttaaaga tgcttcgtgt cagttttgta ttattggtca ttctgaaaga
     1261 agacaaatat ttggacaatc aaatgaagat gtttgtatta aggctatgaa tttaattgca
     1321 aataatatca atccaattat ttgtattgga gaaacttttg aggaaaaaaa tcagaattta
     1381 acaaaagata ttatatatac tcaaatttca aagagcgttc caaaaaatgc tacaaaggat
     1441 tttgtaatta ttgcttacga gcctatttgg gcgattggaa caggtaaaat cccttcttta
     1501 gaagaaattg acaatattca ttcttttatt aaaaatgaaa ttcaaaatat tgaaaactat
     1561 aaa
//
LOCUS       DTVV01000012            1760 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1060131, whole genome shotgun sequence.
ACCESSION   DTVV01000012 DTVV01000000
VERSION     DTVV01000012.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1060131"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="EYO86_00160"
     CDS             <1..472
                     /locus_tag="EYO86_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC41483.1"
                     /translation="FNHLRMKLGFAPMLFTSDVKLAMPSIIFVDIWQWTPFIFLMMLA
                     GLASLPKEPYEAAQIDGASSWQQFRDLTWKFLQPVIIITLLLRIMDAFRLFDQVFILT
                     RGGPASVTDTLSYYIYKVAFRFWDIGWAAAMSLFMLAVTLIISAWFVGKLNIEK"
     gene            459..1298
                     /locus_tag="EYO86_00165"
     CDS             459..1298
                     /locus_tag="EYO86_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC41484.1"
                     /translation="MLKNNFKKPIVFLSIVFALFYTLFPFIYMISVSFKIRKDIFAMP
                     PKYFNFDATLYNYWFTFFDQIPFFKFMINSFIITSVSTIAALILGTLAGYSLARFRYP
                     GNLKYHLSFWILSTRMLPPIVVVIPLFLFFSFFNLVNTKLALIIAYTAFNIPFVAWMM
                     RSFFLDIPKELEESAMVDGDTRMGSFRRIILPLAKPGLAATSIFCLILSWNEFLFAVI
                     LTETEISNTVTFAIALGVTQYQSNWGYMAASATISVIPIIIFAFIVQKHLVRGLSFGA
                     IKG"
     gene            1303..>1760
                     /locus_tag="EYO86_00170"
     CDS             1303..>1760
                     /locus_tag="EYO86_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC41485.1"
                     /translation="MANVIFENITKKFDDVTAVEDFSLEVQDKEFLVLLGPSGCGKTT
                     TMRIVAGLEKPNSGKVLIDAKDVTDYAPKDRDVAMVFQSYALYPHMTVFQNIEYPLKI
                     KNVPKEKRKDQVLETAKKVQLEGLLKRLPKQLSGGQQQRVALARVIIRNP"
BASE COUNT          538 a          272 c          309 g          641 t
ORIGIN      
        1 atttaatcat ttaagaatga agcttggttt tgcacctatg ctattcactt cagatgtaaa
       61 actagctatg ccatcaataa tttttgttga tatatggcaa tggacaccat ttattttttt
      121 aatgatgctc gctgggctag caagtcttcc taaggagcct tatgaggccg ctcaaattga
      181 tggcgcatcc tcttggcaac aattcaggga tttgacttgg aaatttttac aaccagttat
      241 cattattact cttttactta gaataatgga tgcgtttaga ctctttgatc aagtatttat
      301 attaactaga ggtgggccag ccagtgtcac ggatacgctc agttactata tctacaaagt
      361 agcttttaga ttttgggata taggatgggc tgctgcaatg tcattgttta tgttagctgt
      421 aaccttaata attagcgctt ggtttgttgg aaagttaaat attgaaaaat aattttaaaa
      481 aaccaattgt ttttttatct attgtgtttg ctctttttta tacacttttt ccatttattt
      541 atatgataag cgtatccttc aaaatacgga aagacatttt tgctatgcca ccaaagtact
      601 ttaattttga tgcaacccta tataactact ggtttacttt ttttgaccaa ataccttttt
      661 tcaagtttat gattaatagt tttattataa cttcagtttc tactattgct gctttgattc
      721 tcggaacact ggctggttat tctttggcaa ggtttagata tccaggcaat cttaaatatc
      781 acttgtcttt ttggatttta tcaacaagaa tgttgccacc tattgttgtt gtcataccac
      841 ttttcttgtt tttttctttt ttcaatttag ttaatacaaa attagctctt attattgctt
      901 atacagcttt taatattcct tttgtagcct ggatgatgcg aagtttcttt ctggatattc
      961 ccaaagaatt agaagaatca gctatggttg atggggatac tagaatggga tcatttcgta
     1021 gaattatatt acctttggcc aagccaggac ttgctgcaac atctatattt tgtttaatct
     1081 tatcttggaa tgagttttta tttgcagtga tattaactga aactgaaata tcaaacacgg
     1141 ttacttttgc tatagcacta ggtgttacac agtatcagtc taactggggg tatatggctg
     1201 catcagccac aataagtgtt atccccatca taatttttgc atttattgta caaaaacatt
     1261 tagttcgagg gctctcgttt ggagcaataa aaggataaca taatggccaa tgtaattttt
     1321 gaaaatataa caaaaaaatt tgatgatgta accgctgtgg aagatttttc actagaagtt
     1381 caggataaag aatttttagt tttgcttggc ccatcaggat gtggaaaaac tacaacaatg
     1441 aggatagtgg ctggtcttga aaaacctaat tctggcaaag tacttattga tgctaaagat
     1501 gttactgatt atgcaccaaa ggatagagat gtcgcaatgg tatttcaaag ttatgctctt
     1561 tatccgcata tgactgtttt tcagaatatt gaatatccat taaaaataaa aaatgtgcct
     1621 aaagaaaaaa gaaaagatca agttcttgaa actgcaaaaa aggttcaatt agaaggtttg
     1681 ctaaagcgtt tacctaaaca attatcaggt ggtcaacaac aaagagttgc tttggcacga
     1741 gttattatta gaaatccaaa
//
LOCUS       DTVV01000013            1530 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1062654, whole genome shotgun sequence.
ACCESSION   DTVV01000013 DTVV01000000
VERSION     DTVV01000013.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1062654"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="EYO86_00175"
     CDS             <1..1347
                     /locus_tag="EYO86_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HIC41486.1"
                     /translation="KYAKLGYTAVFEPASVPANARSTIIEMSDIPLIDKGCYTVLGND
                     DFLLNLIQKKTSQKKINDYVAWILTSTKGLGIKIVNPGGINAFKFNQRELDLDAKNKK
                     YGITPRSILKILTKSLIDLKAPHPIHVHGCNLGVPGNVKTTLKQISAVEGKPMHLTHI
                     QYHSYSNEGDRNFSSGAAILAESINKNKNISCDIGQVMFGQTVTTSADTMSQYRNHSF
                     AHPKKWICADIECEAGCGIIPFEYRDKNYVNALQWAIGLELFLLIKDPWRVYLTTDHP
                     NGAAFTAYPRLIKLLMDRSFRNEELEKINKDSQNNSILKSLKREYSLYEIAILTRSGP
                     AKILGLNNIGHLGEGAKADITVYTEHQDKEKMFENPFLVFKDGQIIVKNGTIKKVING
                     KLYIAEAEYDQSIKKEISIYFEKYIGRKMSSFEIQNQELWDHGLELEQIRCSRNDY"
     gene            1337..>1530
                     /locus_tag="EYO86_00180"
     CDS             1337..>1530
                     /locus_tag="EYO86_00180"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817382.1"
                     /note="catalyzes the transfer of a formyl group from
                     formylmethanofuran to tetrahydromethanopterin
                     tetrahydromethanopterin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HIC41487.1"
                     /translation="MIINGIKIEKTFAEAFSMKATRIIVTAETKYWVSKAVESMTGFA
                     TSVIACGCEGGIEKEIKESST"
BASE COUNT          607 a          196 c          271 g          456 t
ORIGIN      
        1 aagtatgcta aactaggata tacagcagtt tttgaaccag cgagtgttcc agcaaatgca
       61 agatctacaa taatagaaat gagtgacata ccgttaatcg ataaaggatg ctatactgtt
      121 ctgggtaacg atgatttttt attgaatctt atacaaaaaa aaacaagtca aaaaaaaatt
      181 aatgactatg ttgcatggat tttaacatca acaaaaggat taggaataaa aattgttaat
      241 ccgggtggaa ttaatgcatt taaatttaac caacgagaat tagatttaga tgcaaaaaat
      301 aaaaaatatg gaatcacacc aagatcaata ttaaaaattt taacaaaatc tcttatagat
      361 ttaaaagcac cccatcccat acatgtacat ggttgtaatt taggtgttcc tggaaacgtt
      421 aaaactactc taaagcaaat tagtgctgtt gaagggaaac ccatgcatct aactcatatt
      481 caataccatt catatagtaa tgaaggagat agaaattttt catctggagc tgcaatactt
      541 gcagaatcta ttaataaaaa taaaaatatt tcttgtgata ttgggcaagt tatgtttggg
      601 caaacagtaa cgacttcagc tgatacaatg agccagtata gaaatcattc ttttgctcat
      661 ccaaaaaaat ggatttgtgc agatatcgaa tgcgaagcag gttgcggaat aatccctttt
      721 gaatatcgtg ataaaaatta tgttaatgca cttcaatggg caattgggct agaattattt
      781 ttgcttataa aggatccttg gagagtgtat ttaacaactg accatcctaa tggagctgct
      841 tttacagctt atccaagatt aattaagctt cttatggata gaagttttcg taacgaagaa
      901 ttagagaaaa ttaataaaga ttctcaaaat aattctattt taaaaagttt aaaaagagaa
      961 tatagccttt atgaaatagc aattttaaca agatctggtc ctgcaaaaat attaggttta
     1021 aataacatag gccatctagg tgaaggggca aaagcagaca taacagtata tactgaacat
     1081 caagataaag aaaaaatgtt cgaaaaccct tttttagttt ttaaggatgg ccaaataatt
     1141 gtaaaaaatg gaacaattaa gaaagttata aatggtaaat tatatattgc agaagcagaa
     1201 tatgaccaaa gtattaaaaa agaaattagt atatattttg aaaaatacat aggaagaaaa
     1261 atgtcttctt ttgaaataca aaatcaggaa ctttgggatc atggactaga attagaacag
     1321 attcgttgta gtagaaatga ttattaatgg aataaaaata gaaaaaactt ttgctgaagc
     1381 tttttcaatg aaagccacga gaattattgt tacagcagaa acaaaatatt gggtttctaa
     1441 agctgttgaa agtatgacgg gttttgctac ttcagtaatt gcttgtggtt gtgagggcgg
     1501 cattgaaaaa gaaataaaag agtcatcgac
//
LOCUS       DTVV01000014            1296 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1063460, whole genome shotgun sequence.
ACCESSION   DTVV01000014 DTVV01000000
VERSION     DTVV01000014.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1063460"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="EYO86_00185"
     CDS             complement(<1..349)
                     /locus_tag="EYO86_00185"
                     /inference="COORDINATES: protein motif:HMM:PF01040.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIC41488.1"
                     /translation="MLDKIIPKKIKHLIDLIRLDKPIGFLLLMWPCWFALANLPQDNA
                     ELTYWYVYFVIGAFLMRSAGCIINDFVDINLDKNVERTAERPLTSKKVSITEAIVLLL
                     VLLFFSFYILLQFN"
     gene            388..1122
                     /locus_tag="EYO86_00190"
     CDS             388..1122
                     /locus_tag="EYO86_00190"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:PRK11713.3-5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIC41489.1"
                     /translation="MKKSKTRIFVNKSISSNLIIYIKDKQHHFLKNVLRIKVNDEINI
                     FDGITGEWESTVMSINRENTVLRVTNIINKIKKSNDIWLVFSPIKQHRMSLAIQKATE
                     LGVSKIIPCITEFTNIRKINAKNLHDNAIEAAEQSERLDVPRIEKQVDLTTLLSNWPE
                     DRKLIYCDEKIKEKRSIIDLLTLFKDDENKWAVLIGPEGGFSDSEQELITKSKNVLSV
                     SLGDRLLRSDTAITVSLFCIQEILSK"
BASE COUNT          510 a          174 c          202 g          410 t
ORIGIN      
        1 aattgaattg caaaagaata taaaaagaaa aaaataaaag aactaaaagc aatacaattg
       61 cctcagtaat ggagactttt tttgatgtta gtggtctttc agcagttctt tcaacatttt
      121 tatctaaatt aatatcaaca aaatcattaa ttatgcaacc cgcacttcgc attaaaaagg
      181 ctccaataac aaaatataca taccaatatg ttagttctgc attgtcttgt ggaaggtttg
      241 ctagcgcaaa ccagcatggc cacattaaca ataaaaaacc tataggtttg tccaatctaa
      301 ttagatcaat taaatgttta attttttttg gaataatttt atccaacata ataagaaaaa
      361 tgataatgtg ggcattatat taaaacaatg aaaaagtcca aaacgcgcat ttttgtaaac
      421 aaatccatat cttcaaattt aattatttat ataaaagaca aacaacatca ttttttaaaa
      481 aatgttctaa gaataaaagt taatgatgaa ataaatatat ttgatggcat tactggagag
      541 tgggagtcaa cagtaatgtc gataaacaga gaaaataccg tattacgtgt aactaatatt
      601 attaataaga taaaaaaatc taatgatatt tggctggtat tctcacctat taaacaacat
      661 cgaatgagtt tggctatcca aaaagctaca gaacttggcg tatcaaaaat tatcccatgc
      721 attactgaat ttacaaatat tagaaagatt aatgctaaaa atctacatga caatgcaata
      781 gaagctgccg aacaatcgga gcgactcgat gttccacgaa tagaaaagca agttgatctt
      841 actacattgt tatcaaattg gcctgaagat agaaagttaa tttattgtga tgaaaaaatc
      901 aaagaaaaga gatcaataat agacttattg acacttttta aagatgatga aaataagtgg
      961 gcagtgttga taggacctga aggaggattt tccgactcag aacaagaatt aattaccaaa
     1021 agtaaaaatg ttttatcggt ttcgttaggt gatagactgc ttcgttctga cacagcaata
     1081 accgtttctt tattttgcat tcaagaaata ttatccaaat aatagtattt cataaatata
     1141 cgaattgatt tatatataat ttcaatatta tgactgtctt gcttttaatg gttttgggga
     1201 ttttcttgtt ttgttatttt gtggcaacaa gggtatggat taaggttgat gaacaaaaaa
     1261 atgtatcttc atccaatgat aacagtaaag gaagaa
//
LOCUS       DTVV01000015            2555 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1064723, whole genome shotgun sequence.
ACCESSION   DTVV01000015 DTVV01000000
VERSION     DTVV01000015.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1064723"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(95..1864)
                     /locus_tag="EYO86_00195"
     CDS             complement(95..1864)
                     /locus_tag="EYO86_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013695958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIC41490.1"
                     /translation="MEDKKYIKTFKLLLPYLWPKKRNDLRTRVSFAVVALVLAKIASV
                     STPLVLGEAVNSLTELSAGINLFMLVPVALIVGYGVARIISFTFVAIRDALFSKVSQH
                     SIRQISLNMFKHLHNLSLQFHLNRQTGALAKYIDRGTKGIDFLLRYVLFNVVPTFFEI
                     FLVSGILFYLYGPWYAVVTLTTIGLYSYLTFQITEWRNVFRKRMNQADNNISTKMIDS
                     LLNFETVKYFNNEAYEFNRLDKSLEDYELAANQSRHSLSLLNIAQTFIIMLGITIMLV
                     MSAFGIKNGDIDVGGFVVINAYMLQLYQPLNFLGSVYREIRQALTDMENMFSLLEVSQ
                     VSKDDLEDMPQSNVAEIRFDKVSFDYDIRRTIIKNISFTVPNGKKVAIVGPTGAGKST
                     ISRLLFKFYDPKEGGVYINNININKISQESLRKMIGVVPQDTVLFNDTIYYNIAYGNT
                     DASKEEVIIAAKNADIHNFISTLPDGYETIVGERGLKLSGGEKQRVAIARTILKNPKI
                     FFFDEATSALDSSTEKEIQKNLESVSKDKTTLIIAHRLSTAANADNIIVLDKGIIIEQ
                     GTHKSLLLEKGKYFEMWGKQKLS"
     gene            complement(1873..2364)
                     /locus_tag="EYO86_00200"
     CDS             complement(1873..2364)
                     /locus_tag="EYO86_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HIC41491.1"
                     /translation="MQSPIPSFINSLRCLSSILKKAEQHCKDRKIDPETLLTTRLFPD
                     MLNLKTNVFIACDTAKGLAARLSQTENPKYEDNESTFEELQTRISKTIKFMESVPEAS
                     FEGAETREVILRFGKQETKFIGADYLSGFATPNFYFHMATVHGILRHNGVEIGKRDFL
                     GPQ"
BASE COUNT          842 a          454 c          365 g          894 t
ORIGIN      
        1 agacagaaaa tagtccctaa aaagaaataa taaaaatata gcaacaccaa accacaataa
       61 atgatattta tcttcttttc tttttgtaaa agtcttagct aagcttttgt tttccccaca
      121 tttcaaaata cttacctttt tcaagtaata aacttttgtg tgtcccttgt tcaatgataa
      181 tccctttatc aagaacaatg atattatctg catttgcagc tgtagataaa cgatgtgcaa
      241 ttattaaagt tgttttatct ttagagacac tctccaagtt tttttgtatt tctttttctg
      301 tgctactatc tagtgcagat gttgcttcat caaagaaaaa tatttttgga ttttttaaaa
      361 tagttcttgc aatagcaact ctttgttttt ctcctcctga taactttaat cctctctctc
      421 ctacaattgt ttcataacca tctggtaaag ttgaaataaa attatgtata tcagcatttt
      481 tagcagcgat aataacttct tctttagagg catccgtatt tccgtaagcg atgttgtaat
      541 agattgtgtc attaaataag actgtatcct gtggaactac accaatcatt tttcttaaag
      601 attcttgaga aattttattt atatttatat tgttaatata aacaccccct tctttggggt
      661 cgtaaaattt aaataataat ctacttattg ttgatttgcc cgcaccagtt ggccctacta
      721 ttgcaacttt ttttccgtta ggaacggtaa aagaaatatt tttaattatg gttcgtctta
      781 tatcataatc aaaactgact ttatcaaatc taatctcagc aacattgctt tgcggcatat
      841 cttcaaggtc atcttttgat acttgagata cctccagtaa actaaacata ttctccatgt
      901 ctgttaatgc ttgtctaatc tctctgtaga cagaacctaa aaaatttaac ggttgatata
      961 attgaagcat gtaggcatta ataacaacaa acccaccaac atcgatatct ccatttttaa
     1021 taccaaaagc tgacattacc aacataatgg taatacccag catgatgata aatgtttgag
     1081 ctatgtttaa taatgaaagc gaatgcctgc tttgatttgc agctaattca taatcttcta
     1141 aagatttatc aagcctatta aattcataag cttcattatt gaaatattta actgtttcaa
     1201 agtttaataa actatcaatc atttttgtgc tgatattatt atcggcctgg ttcattcttt
     1261 ttctaaatac gtttctccat tcagttattt gaaaagttaa ataagaataa agaccaatag
     1321 ttgtaagcgt tacaacggca taccagggtc cataaagata aaataaaatt cctgaaacta
     1381 agaaaatttc aaaaaaagta ggaacaacat taaacaaaac ataccttaaa agaaaatcta
     1441 ttcccttggt tcccctatct atatattttg ccagtgcacc tgtttgtctg tttaaatgaa
     1501 attgtaatga cagattgtgt aaatgtttaa acatatttaa agatatctgt ctgatggaat
     1561 gttgagaaac ttttgaaaac aaagcatctc taattgcaac gaatgtaaaa gaaattattc
     1621 ttgctactcc gtaaccaact attaaagcaa caggaaccag cataaataaa ttaatacctg
     1681 cactcaattc tgttagagaa tttactgcct cacctaacac tagaggtgta ctaacacttg
     1741 ctatttttgc tagaactaaa gcaacaacag caaagctaac tctcgtcctt aaatcgtttc
     1801 tttttttagg ccacaagtaa ggaagtaata atttaaaagt tttaatatat tttttatctt
     1861 ccattactta cattattgtg gaccaagaaa gtctcttttg ccaatttcaa ctccattatg
     1921 acgcaaaatt ccatgtaccg ttgccatgtg gaagtaaaag tttggcgtag caaagccgga
     1981 aagataatcg gcaccgataa actttgtttc ctgttttcca aaacgcaaaa taacttctcg
     2041 ggtctcggcg ccttcaaatg aagcttctgg cacagactcc ataaacttga tcgttttgct
     2101 aatacgggtc tgtagttcct caaaggttga ctcgttatct tcatatttag gattttccgt
     2161 ttgagataaa cgggcggcta gacccttagc ggtatcacaa gcgataaaca cattggtttt
     2221 taaattaagc atgtctggaa ataaacgtgt cgttaataga gtttcgggat ctattttacg
     2281 atctttacaa tgttgttcag cttttttcag aatagaagaa aggcaacgta aagaatttat
     2341 aaagcttggt ataggtgatt gcatctattt cctttctttt aaaatttttc tactgaagtt
     2401 ctcaattcta attatttttt tcatattttt ttaggatata atccttagaa actagcgtca
     2461 atactcacgg accatggctc aaatctttcc agcgaaatca accttaatac aagcaaagac
     2521 tctgcattaa atatcaggta tcatgaagca tgaag
//
LOCUS       DTVV01000016            2051 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1066139, whole genome shotgun sequence.
ACCESSION   DTVV01000016 DTVV01000000
VERSION     DTVV01000016.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1066139"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            70..942
                     /locus_tag="EYO86_00205"
     CDS             70..942
                     /locus_tag="EYO86_00205"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIC41492.1"
                     /translation="MFQGSIPALITPFKNGNIDYESFYKIIEWSLSEGSHGFVPCGTT
                     GESPTLSHQEHIKVVDECVRIVDKRVPVIAGTGSNNTVEAIEFTNQAEKSGADAALVV
                     TPYYNKPTQEGLQAHFKKIAESTSLPIIIYNIPGRSIVDMTIDTMSVLSNISNIVGVK
                     DATNDLFRPLLTQTKIQKDFCYLSGEDGTALAYLAQGGQGCISVTANIAPKLCSDMQN
                     LWRERKINLAQDINFKLSKIHHVLFVESSPGPVKYAAELLNLCSAETRLPLVSIKDST
                     KALVKECLVEIGLI"
     gene            952..1404
                     /gene="smpB"
                     /locus_tag="EYO86_00210"
     CDS             952..1404
                     /gene="smpB"
                     /locus_tag="EYO86_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIC41493.1"
                     /translation="MQIIAANKKAFYDYLIGDRFEAGLVLTGPEVKSLRINTGSIKES
                     YIIEKDGELWSTNCHIKKYSSSKDVENSTTRERKILVKKKELNKIIGASRREGMTVVP
                     IKLYFNDKGLVKLTIALAKGKKKHDKRASIKDKEWVINKQRLIKNRSL"
     gene            1449..1895
                     /locus_tag="EYO86_00215"
     CDS             1449..1895
                     /locus_tag="EYO86_00215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008885085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIC41494.1"
                     /translation="MARITVEDCIDKLPSRFELVLVASQRARKLYSGETPTVVTENDK
                     NTVIALREIADSTLTADEMRENLIQEYQTVTISDEADEILELESSNETTNKNEESKSY
                     VSDVEEEMKELVSSEDSEIKEQYEIRDDIEKNTVSDETEENTQSLE"
BASE COUNT          763 a          280 c          372 g          636 t
ORIGIN      
        1 attgccgaag ggagtaatcc cttccgtggg ttcgaatccc actctctccg ccaaaatatt
       61 attattataa tgttccaggg aagtatacca gcactcatca ctccatttaa aaatggaaat
      121 attgattatg aatcttttta taaaataatt gaatggtctt tgagtgaagg atcccatggc
      181 tttgttcctt gcggcacaac tggtgaatct ccaaccctta gtcatcaaga gcacataaag
      241 gttgttgatg agtgtgttag gattgtagat aaaagagtac ctgttattgc tggaactggt
      301 tcaaataata cagttgaagc tatagagttt acaaatcaag cagaaaaatc tggagccgat
      361 gcagctttag tcgttacacc ttattataac aagccaacac aagaaggttt gcaagctcat
      421 tttaaaaaga ttgctgaatc aacatctctt ccaataatta tttataatat tcctggacgc
      481 tctatagttg atatgacaat tgatacgatg agtgttttat caaatatttc taatattgtc
      541 ggtgttaaag atgctaccaa tgatttattt agaccacttc taactcaaac aaaaatacaa
      601 aaagattttt gttatttatc aggcgaagat ggcaccgctt tggcttattt ggcacagggt
      661 ggtcaaggat gtatttcagt aacagctaac attgctccaa aattatgctc agatatgcaa
      721 aatctatgga gagagagaaa aattaattta gctcaagata taaattttaa attatctaaa
      781 attcaccatg ttctttttgt tgagtcaagt ccagggccag ttaaatatgc agctgaatta
      841 ttaaatttat gtagtgctga aacacgtcta cctttggttt ctatcaaaga tagtacaaaa
      901 gcattagtta aggaatgtct agttgagatt ggattaatct aattattttt tatgcaaata
      961 atagcagcaa ataaaaaggc tttttacgat tatttgattg gtgatagatt tgaagcgggt
     1021 ttagttttaa ccggacctga ggttaaatct ttaagaatca acacaggttc tattaaagag
     1081 tcgtacataa ttgagaaaga tggagagctt tggtcaacaa attgtcatat aaaaaagtac
     1141 tcttcatcta aagatgtaga aaacagtact actagagaaa gaaagatact tgtaaagaag
     1201 aaagaactta ataagattat aggcgcctca agaagagaag gtatgacagt agtaccaata
     1261 aaactgtatt ttaatgacaa aggtttggta aaattaacaa tagctctagc taaaggtaaa
     1321 aaaaaacatg ataaaagagc ttctataaaa gataaggagt gggtgattaa caaacaaaga
     1381 ttgataaaaa accgatcttt ataagaatat ttcaatttac ttttatttta taataactat
     1441 aagcccctat ggcgagaatt acagtagaag attgtattga taaattacca agtagatttg
     1501 agttggtttt agttgcttcg cagcgtgcga gaaaattata ttcaggtgag acaccaacag
     1561 ttgtaactga aaatgataag aatacagtaa ttgcgcttag agagattgca gattctactt
     1621 taactgcgga tgaaatgaga gaaaatttaa ttcaagaata tcaaacagta acaataagtg
     1681 atgaagcaga tgaaattctc gaattagagt cttcgaatga gacaacaaat aaaaatgaag
     1741 aatctaaatc ttatgtttca gacgtagaag aggagatgaa agaacttgtt tcttctgagg
     1801 acagtgagat taaagagcaa tatgaaatta gggatgatat agaaaagaat actgtatcag
     1861 atgaaactga agaaaataca caatcattag agtaatcgtc tctatttatt tattttttct
     1921 cattaaaata taatgttatt tttttatgga aaataaaaaa ataaaagaat taattgatct
     1981 tgctatttca tatattccaa aatctgaagt tgaagtaatc tctaaggcca ttacttttag
     2041 cagtcaagct c
//
LOCUS       DTVV01000017            1622 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1066449, whole genome shotgun sequence.
ACCESSION   DTVV01000017 DTVV01000000
VERSION     DTVV01000017.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1066449"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="EYO86_00220"
     CDS             <1..1166
                     /locus_tag="EYO86_00220"
                     /inference="COORDINATES: protein motif:HMM:PF01551.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HIC41495.1"
                     /translation="KLHINKDINEEKNLFVTQNIHENEKQKVKKIIITVKKNDTFSKI
                     IDPFFHNNRIKNSIIEELNKVYNLRNLGIGQKIYFYKDEENNTNKIVLPISFSTDLII
                     IVSNGSISLSKEKISITKETISSEFTIFSSLYEDGIKSGIPLKILTDAIRLYSFDIDF
                     QRDIQKNTKFSISYEELFNDERKNITYGDIQYINLKLQNNTLEYFQFKTDDGFFDYFN
                     QQGKNVKKSILKTPIDGARLSSNFGMRKHPILGYSKMHKGVDFAAPKGTPVYAGGNGI
                     IEYASHNGGYGKYIRIRHNNAYKTVYAHLLGFQKGVSKGKRVNQGDIIGYVGSTGSST
                     GPHLHYEILFQNKQINPMKMKLPSGKILSGSELERFKDEAKAIYAKYLFTLYE"
     gene            complement(1167..1622)
                     /locus_tag="EYO86_00225"
     CDS             complement(1167..1622)
                     /locus_tag="EYO86_00225"
                     /inference="COORDINATES: protein motif:HMM:PF13763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HIC41496.1"
                     /translation="MYIKKNARVTFRSNRRSNFKRNNNYSGNKPRNKGNVMQLYNKYL
                     KLAKEFSSSGDRIQSEYYYQFTDHYYRLMLELGITLEDNETTQDNKISSDVNHENQIS
                     NENNNEKNDSEVNDFNETSDSDDVDSIESIPFMAEPAKKKSTKAKKASA"
BASE COUNT          603 a          229 c          238 g          552 t
ORIGIN      
        1 aaaaattaca tatcaataaa gatataaatg aagaaaaaaa tttatttgta actcaaaata
       61 ttcacgaaaa tgaaaaacaa aaggtaaaaa aaataattat cactgtcaaa aaaaatgata
      121 cattttccaa aatcattgat ccattctttc ataataatag aattaaaaat tcaataattg
      181 aagagttgaa taaagtatat aatttgcgta atcttggtat aggacaaaaa atatattttt
      241 ataaggatga agagaacaat actaataaaa tagtcctacc aattagtttt tcaactgacc
      301 taataattat tgtatcaaat ggttctattt ctttatcgaa agaaaaaatt agtattacta
      361 aagaaactat ttcatcagaa ttcactattt tttcatcttt atatgaagat gggataaaat
      421 ctggcattcc attaaaaatt ttaacagatg caatacgatt atatagcttt gatatagatt
      481 ttcagagaga tattcaaaaa aatacaaaat tttctatttc ttacgaagag ttgtttaatg
      541 atgaaagaaa aaatattacc tatggagata tacaatatat caatctgaaa ctccaaaata
      601 ataccttaga atatttccaa tttaaaactg acgatggatt ttttgattat ttcaatcaac
      661 agggaaaaaa tgttaaaaaa tcaatattga aaactcctat tgatggagct cgattatcgt
      721 ctaattttgg aatgagaaaa catcctattc ttggttacag taaaatgcac aaaggggtag
      781 attttgctgc tcccaagggc actcccgtct atgctggagg taatggtata attgagtatg
      841 cgagtcataa cggagggtat ggaaaatata ttcgtattcg ccacaataac gcatacaaaa
      901 cagtgtacgc acatttatta ggcttccaaa aaggggtatc caaaggcaaa agagttaatc
      961 agggagatat tattggttac gttggtagta caggaagttc cacgggaccc catcttcatt
     1021 acgaaatctt gtttcaaaat aaacaaataa atccaatgaa aatgaaatta ccctctggca
     1081 aaatattatc gggaagtgaa cttgaaagat ttaaagatga agctaaagct atttatgcaa
     1141 aatatctgtt taccctatat gagtgattag gcagatgcct tttttgcttt agttgatttt
     1201 tttttagctg gttcagccat aaaagggatt gactcaattg aatcgacatc atcactatca
     1261 gatgtttcat tgaaatcatt gacttcggaa tcattttttt cattgttatt ttcgttagaa
     1321 atctgattct catgatttac atcactagaa attttattat cttgtgttgt ctcattgtct
     1381 tcaagagtaa tgcccaactc taacattaat cgataatagt gatctgtaaa ttgataataa
     1441 tattctgact gtatcctatc tccagaagac gaaaactctt tagcaagttt tagatattta
     1501 ttatatagct gcataacatt gcctttattt cttggcttat taccagaata gttattattt
     1561 cgtttaaaat tcgatcttct atttgatcta aacgtcactc gcgcattttt ttttatgtac
     1621 at
//
LOCUS       DTVV01000018            1743 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1067188, whole genome shotgun sequence.
ACCESSION   DTVV01000018 DTVV01000000
VERSION     DTVV01000018.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1067188"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="EYO86_00230"
     CDS             complement(<1..298)
                     /locus_tag="EYO86_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC41497.1"
                     /translation="MQLPNNGFDDRLTYYELNNDSLGTPTIFIHGVGLNNTMWLPQKK
                     YFQNDKVVFYDLLNHGKSKKGYKELKFENFNNQLMQLIKFLNIKKFNLIGFSVGA"
     gene            complement(298..>1743)
                     /locus_tag="EYO86_00235"
     CDS             complement(298..>1743)
                     /locus_tag="EYO86_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIC41498.1"
                     /translation="ATNLVTGIAQANAAFSPVISLAGDIASEHKGKDAFQEIDQQALF
                     EPITKKTFSVKQTEKIPKILNEAFNLALAPRCGPVHVNLPRDILTNNANFAEFNKHHN
                     NILPKASKDNLTKTIDIILSSSKPVIIAGGGIKNTQGGQEVIHLAKILNIPIVSSAGH
                     GDALPFDESLYAGQMGPRGNPVASDLVKEADVILALGTRLGFNSTFFSYENINEQASI
                     IQVDIEPKSLGRYFPITIGICADAKTTAIELYNEISLKKETIKIDSWTKNFLDKRKAY
                     LKKRDAEADFNPHLIQPSGLFKILRETLPKNSAVTLDAGTLCLQATDMLNFYHPRSLF
                     TPLDFGLVGFSFACGLGVKLAKPDCPVISLMGDGGFGMTISELSTAVQHKINTVTIVM
                     NNKSWGAEKAYQRDFYEKRYIGANIDSPPFEKVAELYGAAGFHVNKISELKDVINTAL
                     QCEKPAIINVDVDPSALYSFRTDSFKHKQKS"
BASE COUNT          524 a          297 c          321 g          601 t
ORIGIN      
        1 aagctccgac agaaaatccg atgagattaa atttttttat atttaggaat ttaatcaatt
       61 gcataagttg attattgaag ttttcaaatt ttagttcttt gtagcccttc tttgattttc
      121 catggtttaa taaatcataa aaaacaactt tatcattttg aaaatatttt ttttggggca
      181 gccacattgt gttatttagg ccaactccat gtataaaaat agtgggcgtg cccaaactgt
      241 cattatttag ttcgtagtat gttaaacgat cgtcaaaacc attattaggt aattgcatta
      301 agatttctgt ttgtgtttaa atgaatccgt tcgaaaacta taaagagcgc tggggtcaac
      361 atcaacatta atgatagctg gtttttcgca ttgaagagcg gtattgatga catcctttaa
      421 ttcagatatt ttgtttacat gaaatcctgc ggccccgtac agttcagcca ctttttcaaa
      481 aggagggcta tctatgttcg cgccaatgta tcttttttca taaaaatccc tttgatacgc
      541 cttttctgca ccccatgatt tgttattcat gacaatggta acagtattta ttttgtgttg
      601 taccgcagta ctaagttcag aaattgtcat tccaaaacca ccgtctccca ttaaacttat
      661 cacgggacaa tcgggtttgg ctagctttac acccaaacca caagcaaagg aaaaacccac
      721 taacccaaaa tctaagggtg tgaataaaga tcgaggatgg taaaaattta gcatatccgt
      781 tgcttgtaga caaagtgttc cggcatcaag agttacagca gaattttttg gaagagtttc
      841 gcgcaaaatt ttaaaaagcc ctgaaggttg aattaaatga gggttaaaat ctgcttctgc
      901 atctcttttt ttaagataag cttttcgttt atctaaaaaa ttttttgtcc aactgtcaat
      961 tttaatagtt tcttttttaa gtgatatttc attgtataat tctattgctg ttgttttggc
     1021 atcagcacaa ataccaatag taattgggaa ataacgacct aaagatttgg gctcaatgtc
     1081 tacctggatg atggaggctt gttcatttat attttcgtaa gaaaaaaaag tggaattaaa
     1141 tccaagacga gttcccaaag ctaaaattac atccgcttct ttaaccagat ctgaggcgac
     1201 aggatttcct ctgggaccca tttgacccgc atataaactt tcatcaaacg gaagtgcatc
     1261 cccatgtcca gcggaagata caattggaat gtttaatatt ttggcaagat gaattacctc
     1321 ctgaccgcct tgtgtatttt taattcctcc tcccgcgata attacaggtt tggaagatga
     1381 taaaataata tctatcgttt ttgttaaatt gtcttttgat gcttttggta aaatattatt
     1441 atgatgttta ttaaattctg caaagttagc attgttggtt aaaatatctc gaggaagatt
     1501 gacgtgcaca ggtccacatc taggagctaa ggctagatta aaagcttcat tgagtatttt
     1561 gggtattttt tcagtctgct taactgaaaa ggtttttttt gtaataggtt caaatagtgc
     1621 ttgttgatct atttcttgaa aggcatcttt gcccttatgt tctgaagcaa tatcgcctgc
     1681 aagagagatg acaggggaaa aagcagcatt ggcttgcgct atgcccgtga caagatttgt
     1741 tgc
//
LOCUS       DTVV01000019            3204 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1068800, whole genome shotgun sequence.
ACCESSION   DTVV01000019 DTVV01000000
VERSION     DTVV01000019.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1068800"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="EYO86_00240"
     CDS             complement(<1..767)
                     /locus_tag="EYO86_00240"
                     /inference="COORDINATES: protein motif:HMM:PF13419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HIC41499.1"
                     /translation="MNKTESIVGLKDIIDRYDVFILDQWGVMHNGEEGYTAAIKCVET
                     LYKVNKKLIIISNSSKRKHSTAVRLSDFGYRKIYFLEIMTSGEMIWQSLVNLNYDFTK
                     KLQKNCFHIYDKSKEDGKKYLEGLEQFNFVANIEEADFILGCTPQFNTTTIDYIPLLT
                     KAIKNKLPFVCANPDFVSIENNFGKPIICMGTIAELYKNMGGEVFILGKPSCEIYEES
                     TKKISNIDKSKILAVGDSIHHDIVGANNFGIDSLLLTS"
     gene            complement(764..1819)
                     /locus_tag="EYO86_00245"
     CDS             complement(764..1819)
                     /locus_tag="EYO86_00245"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIC41500.1"
                     /translation="MQNIILKLLRNLPPELAHMLTLKSLQFSFRNKKIFDDPILYQHL
                     FGLDFSNPLGLAAGFDKNVEVVNSLLNLGFGFIEAGTVTPKPQYGNEKPRVFRLVEDS
                     AVINHLGFNNKGSEYAKQKLRTMNLNALSKGIVGINIAINKDTTNYLDDYNYCLEKLG
                     PLAHYVTINISSPNTPGLRDLQNRGQIESLVQTLKGKKNSTPTLESTPIFFKIAPDMN
                     EEQIRDIALMSLANNVDGLVISNTTTDRPKSLKSSFKNEVGGLSGTPLFLKSTLILKK
                     IYSLTNGQIPLIGVGGISNGFECYEKIKAGASLVQLYTAIIYKGPQIISKIKLDLTNA
                     LKTDGYKNVKEAIGKEV"
     gene            complement(1844..>3204)
                     /locus_tag="EYO86_00250"
     CDS             complement(1844..>3204)
                     /locus_tag="EYO86_00250"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIC41501.1"
                     /translation="ETKLFTFSDDMDGLRKIPDNVPNKDMLKKHIGKPLTSIPDPFEK
                     YESFGHHNNALLRFFLDEFNFEYDFVSSTEKYQKGDFDNTLIQILQNHDSILSIILPT
                     LRSERKVTYSPFLPISPISGEVLQVKIEEYRPKTNSIIFVDPANNKKTEIIVTGGNCK
                     LQWKVDWAMRWMALGVDYEMCGKDLTESVELASKICRLMNTQPPVNLIYEMFLDEKGE
                     KISKSVGNGISVNEWLRYASPESLSLYMFQKPKSAKKLFFDVIPKSVDEYFSYLKKFH
                     HDEEKEKFNNPVWHIHDGNPPNANSEITFNSLINLVSVCNSNEKEVIWGFVREYDASL
                     NAKNNQEFDRLIDYAINFYTDFVMPKKKYIPIDNSNQDIFLDIVSFLKKIDADSSSED
                     IQTQIYEIGKKYNFTNLRDYFKLIYQVLFGQEQGPRLGSFIKLFGIEKTISLIEDLLK
                     QK"
BASE COUNT         1052 a          547 c          409 g         1196 t
ORIGIN      
        1 gatgtaagca ataaactatc aataccaaaa ttatttgctc caactatatc atgatgaatg
       61 gagtctccta cggcgagtat ctttgattta tctatatttg atattttttt tgttgattct
      121 tcataaattt cacaactagg tttccctaaa ataaaaactt ctccacccat atttttatat
      181 aattctgcaa tcgtacccat acaaataata ggcttaccaa aattgttttc tatcgaaaca
      241 aaatctggat ttgcgcacac aaaaggtaac ttatttttaa ttgctttcgt taaaagaggt
      301 atataatcta ttgttgttgt attaaattga ggcgtacaac ctaaaataaa atccgcttct
      361 tcaatatttg caacaaagtt aaattgttct aaaccttcta aatatttttt tccatcttct
      421 ttgcttttat cataaatgtg aaaacagttt ttttgtaatt tttttgtaaa atcataattc
      481 aaattaacca gactttgcca aatcatctcc ccgcttgtca taatctccaa aaaataaatt
      541 tttctgtatc cgaaatcaga taatcttaca gctgttgaat gttttctttt ggatgaatta
      601 gagattataa ttaatttttt attaacttta taaagtgttt caacacattt aattgctgct
      661 gtatatccct cttcaccatt atgcataact ccccattgat ctaaaataaa aacatcatat
      721 ctatcaataa tgtcttttag gccaacgata ctctctgttt tattcacact tctttaccaa
      781 ttgcttcttt aacatttttg taaccatcag tttttagtgc attggtaaga tctagtttaa
      841 ttttagaaat aatttggggg cctttataaa taatagcggt atacaactga actaatgatg
      901 ctcccgcttt tattttttca taacactcaa aaccattgct aattccacct actcctatca
      961 aaggaatttg accattggta agagaatata ttttttttaa tattaaggta gattttaaaa
     1021 agagtggggt tccacttaat ccccctacct cattcttaaa agacgatttt aaagattttg
     1081 gtctatctgt ggttgtattg cttattacta aaccatctac attatttgct aaactcatta
     1141 gggctatatc ccttatttgt tcttcattca tatcaggggc aattttaaaa aaaataggcg
     1201 ttgactctaa tgtaggtgta ctgttttttt taccttttaa tgtctgtact aaactctcta
     1261 tttgacctct attttgaaga tcgcgcaatc ccggagtatt tggagatgat atattaatgg
     1321 taacataatg cgctaaggga cccagtttct ctaaacaata attgtaatca tcaagataat
     1381 ttgttgtatc tttatttatt gcaatattta tacctacaat gcctttagaa agagcattaa
     1441 gattcattgt acggagtttt tgttttgcat attcagaacc cttattgtta aatcctaaat
     1501 gatttataac agcagaatct tccaccaacc taaacaccct tggcttttca ttaccatatt
     1561 gtggtttagg agtaactgtt cccgcttcta taaaaccaaa acctaagttt aataaagaat
     1621 taacaacttc aacgttctta tcaaaacctg ctgcaagtcc tagtggatta ctaaaatcta
     1681 aaccaaataa atgttggtat agtattggat cgtcaaatat ttttttattt ctaaagctga
     1741 attgcaatga ttttaaagtt agcatgtggg ctaattcagg gggtaagttt ctaaggagtt
     1801 ttaagattat attttgcact ttatataatt gaacgcacct tattcatttt tgcttaagta
     1861 agtcttcaat caaactaata gttttttcta tgccaaataa cttgataaat gaccctagac
     1921 gaggtccttg ctcttgccca aacaatactt gataaataag tttaaaataa tcccttaaat
     1981 ttgtaaaatt atattttttg cctatttcat aaatttgtgt ttgaatatct tcagatgatg
     2041 agtcggcatc gatttttttt aaaaaactca caatatcaag aaaaatatcc tgattagaat
     2101 tatcaattgg tatatatttt tttttaggca tcacaaaatc tgtataaaaa ttaattgcat
     2161 agtctattaa tcgatcaaat tcttgattat ttttagcatt cagtgatgca tcatattctc
     2221 ttacaaaacc ccaaatcact tctttttcat ttgaattaca aacactaact agatttatta
     2281 gggaattaaa agtaatctca ctgttagcat taggaggatt gccatcatga atatgccata
     2341 caggattatt aaatttttct ttctcttcat catgatgaaa cttttttaaa tatgaaaaat
     2401 actcatcaac gctctttggt ataacatcaa aaaacaattt tttagccgat tttggttttt
     2461 gaaacatata caaagataga ctttcgggag aagcatagcg caaccattca ttgacactaa
     2521 tgccatttcc aacagatttt gaaatttttt cacctttttc atctaaaaac atttcataaa
     2581 ttaaattgac aggcggttgt gtattcatca atcgacaaat ttttgacgcc aattcaacac
     2641 tttctgtcaa atctttgcca cacatttcat aatcaactcc taatgccatc catcgcatag
     2701 cccaatcaac tttccactgt agcttgcaat ttccgcccgt aacaattatc tcagtttttt
     2761 tattattagc tggatctaca aaaataatag aatttgtttt aggtcggtat tcttcaattt
     2821 taacttgcaa aacctcacca ctaataggac ttattggtag aaatggacta taagtgacct
     2881 ttctttcaga ccgtaaagtc ggaagaatta ttgataaaat cgaatcatga ttttgaagaa
     2941 tttggataag tgtattatca aaatcacctt tttgatattt ttctgtcgaa cttacaaaat
     3001 catactcaaa attaaattcg tctaaaaaaa aacgaagtag agcattatta tgatgtccaa
     3061 aactttcata tttctcaaaa ggatctggaa tagatgtcaa aggtttgcca atatgttttt
     3121 ttaacatatc tttatttgga acattatctg gtattttacg caaaccatcc atatcgtcag
     3181 aaaaagtaaa taattttgtc tcgc
//
LOCUS       DTVV01000020            1133 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1069221, whole genome shotgun sequence.
ACCESSION   DTVV01000020 DTVV01000000
VERSION     DTVV01000020.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1069221"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /locus_tag="EYO86_00255"
     CDS             complement(<1..81)
                     /locus_tag="EYO86_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIC41502.1"
                     /translation="MEKVAVVTGTSSGIGFETALALARDGY"
     gene            223..417
                     /locus_tag="EYO86_00260"
     CDS             223..417
                     /locus_tag="EYO86_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41503.1"
                     /translation="MASYASEVASIHKQFNVALKRAKSRQAVLNCYWKHKAQHERLLK
                     KHLKEEIAQVNKIKARIKYK"
     gene            565..>1133
                     /locus_tag="EYO86_00265"
     CDS             565..>1133
                     /locus_tag="EYO86_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HIC41504.1"
                     /translation="MKTIPDETIDLVVTDPPFAINFKAKKANYNRTASRVLSGYNEIN
                     VEDYYKFTNNWMRQVKRVLKKSGSMYVFSGWNNLKDILTALDVNGFTTINHIIWKYQF
                     GVVTAKKFVTSHYHCLFVCKNDKMRKFFPYSRFKKNAKTSDGKSLHYRDKEDVWIIKR
                     EYWTGDDKTPTKLPAEIIKKILQYSSEKN"
BASE COUNT          434 a          179 c          175 g          345 t
ORIGIN      
        1 atatccatct cttgcaagcg ctaaagcagt ctcaaagcct attccactgg aggtccctgt
       61 aacaactgca accttttcca tataggtttc aaataataac tatctaatag ttttatgatc
      121 gcttcgtgca cttgatatct aatagaggtc atatgtagca gcccacttaa tatattgata
      181 ataattcgaa acggttatct agaatcttga tcaacaaaat acatggcaag ttatgcaagt
      241 gaagtagcat caatccacaa acaattcaat gtagcattaa aaagggcaaa atcaaggcag
      301 gcagttttaa actgctattg gaaacataaa gcccagcatg aaagactact aaaaaaacat
      361 cttaaggaag aaatcgctca agttaacaaa ataaaagcta ggatcaaata caaataaact
      421 aatagttttc atcaatatgt ttatttgata agtttggctt gatccgtgtt tttttaatta
      481 attatatgta acatatgatt acattttttg taattgaaaa aatccgttgt gaataaaatc
      541 tacaataaag attgtatcat tggtatgaaa acaattccag acgaaacaat agatttggtt
      601 gtcactgatc ccccatttgc gattaatttc aaagctaaaa aggcaaacta taacagaact
      661 gcttcaagag ttttgtctgg ttacaatgaa attaatgttg aagattatta taaatttaca
      721 aataattgga tgcgccaagt aaagcgtgtt ttaaaaaaat ccggtagtat gtacgtattt
      781 tcaggatgga ataaccttaa agacattcta actgcattag atgttaatgg gtttacaacc
      841 attaaccaca taatctggaa gtaccaattt ggtgtagtta cggcaaaaaa atttgttact
      901 tcccactacc actgtctttt tgtatgtaaa aatgataaaa tgcgaaaatt ttttccttat
      961 tcaagattta aaaaaaatgc caaaacatct gatggaaaaa gccttcacta tcgtgacaaa
     1021 gaagacgttt ggattataaa acgtgaatat tggactggag atgataaaac tccaacaaaa
     1081 cttcctgctg aaataattaa aaaaattctc caatattcta gtgaaaaaaa tga
//
LOCUS       DTVV01000021            1656 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1069553, whole genome shotgun sequence.
ACCESSION   DTVV01000021 DTVV01000000
VERSION     DTVV01000021.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1069553"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            24..1163
                     /locus_tag="EYO86_00270"
     CDS             24..1163
                     /locus_tag="EYO86_00270"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00271.29,HMM:PF03461.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41505.1"
                     /translation="MAISGIKDLCSINSAPEHRLPVETIINEYDESLIQQSIVDELNR
                     GGQVFFLHNRVQSINIIHNQLKKLIPNAKIKIAHGQMNENDLEKTMISFAKREIDILC
                     CTTIIESGLDLPNVNTLIVNNAHKFGLSQLYQLRGRVGRSIKQAYSYFLIPKSTILTP
                     QSEKRLQALKESVELGSGYKIAMRDLEIRGTGNILGKEQSGHVITIGLHLYSKLISEA
                     KKYLTTDDNPILNNNIKNPLPILNMDIKSGLTNEYIKEDKMKFELYRQLSICDQIEDV
                     DKVTNNITDRFGIMNQEAKNLILNTKIQILSANININSINKNKNDLTIRFHSQIGGAK
                     NVLLKSFNNAKLSRSNEIIIKLQDVNSWANEIFNILKKIEGIKNK"
     gene            1173..>1656
                     /locus_tag="EYO86_00275"
     CDS             1173..>1656
                     /locus_tag="EYO86_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41506.1"
                     /translation="MNIVLKLLPLFSAFTWAASGLILKKYKIKNLHLFPLIESSISLI
                     IICIIVTFINHWPVFFSYTTNLYLTLISAYGLSVLGTLLYIESIKRIELGIVFTLATS
                     IQIILIGLLDKFINLYTFNSIFILGSAIIIIGVITINYSYLKLLKTINQNKIKLLGIF
                     S"
BASE COUNT          715 a          208 c          208 g          525 t
ORIGIN      
        1 cacctatccc aagaactcta tatatggcta tatcaggaat aaaagattta tgtagcatta
       61 atagtgcccc tgaacataga ttgccagtag agacaataat taacgaatat gatgaatctt
      121 taatacaaca atcaatagtt gacgaattaa atagaggcgg ccaagttttt tttctgcata
      181 acagagttca gagcataaac attattcata atcaactaaa aaaattaata ccaaatgcaa
      241 aaataaaaat tgcacatggt caaatgaatg aaaatgatct tgaaaaaaca atgataagtt
      301 ttgctaaaag agaaattgac atactatgtt gtaccactat cattgaatct ggattagact
      361 tgcctaatgt aaacacatta atagtgaaca atgctcataa atttggatta tcacaactat
      421 accaacttag aggaagagta ggtcgaagca ttaaacaagc ttacagttat ttcttaatcc
      481 ctaaatcaac aatacttact cctcaatctg aaaaaagact acaggcttta aaagagtcag
      541 tagaattagg ttctggatac aaaatagcaa tgagagattt agaaataagg ggaacaggaa
      601 atatattagg aaaagaacaa agtggtcatg taattacaat tggattacat ttatattcaa
      661 aattaatttc agaagcaaaa aaatatttaa ctacagatga taatcctatt ttaaataata
      721 atattaaaaa tcctttgcca atattaaata tggatattaa atctggatta accaatgaat
      781 atatcaaaga agacaaaatg aaatttgaat tatatcgcca attaagtatt tgtgatcaaa
      841 ttgaagatgt tgataaagtt acaaataata taacagatag atttggaata atgaatcaag
      901 aagctaaaaa tcttatatta aatacaaaaa ttcaaatact aagcgcaaat ataaatataa
      961 attccataaa caaaaataaa aatgatttaa ccatcagatt tcatagtcaa attggcggtg
     1021 caaaaaatgt tctattaaaa tcatttaaca atgcaaaact aagcagatca aatgaaataa
     1081 taataaaatt gcaagatgtt aattcttggg caaatgaaat tttcaatatc ctaaaaaaaa
     1141 tagaggggat taaaaataaa taaaattaaa atatgaatat tgtattaaaa ttattacctt
     1201 tatttagtgc attcacctgg gctgcatcag gattaatact aaaaaaatat aaaataaaaa
     1261 atttacattt atttcctctt attgaaagct caatatcttt aattattatc tgtatcattg
     1321 ttacatttat aaatcattgg ccagtctttt tttcatatac aacaaatcta tacctgacat
     1381 taatttcagc atacggatta agtgtcttag gaacgttgtt atatattgaa tctattaaaa
     1441 gaatagaact tggtatagtg tttaccctag caacaagtat tcaaattatt ttaattggat
     1501 tattagataa atttataaat ttatacactt ttaattctat attcatccta ggttcagcaa
     1561 taataataat aggagtaata actattaatt actcctattt aaaattatta aaaacaataa
     1621 atcaaaataa aattaaatta cttggaatat ttagta
//
LOCUS       DTVV01000022            1042 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1071513, whole genome shotgun sequence.
ACCESSION   DTVV01000022 DTVV01000000
VERSION     DTVV01000022.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1071513"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="EYO86_00280"
     CDS             complement(<1..998)
                     /locus_tag="EYO86_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012179112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HIC41507.1"
                     /translation="MTKIKDIKTTIYKWTGPIVPPAQNFCTNPTDLLREKGDKMKSFR
                     FHEWLVCEVIADDGTVGIGNAALAPQVTKKAIDCYLKDLVIEEDPFDYEYLWEKMYRN
                     TMAWGRKGIGMIGISAIDLGIWDLMGKIKNKPVFKLLGNRTKEKIPVYASKLYSQPIN
                     ELQKEAEKYIKEGFKMFKMRFGWGPKDGPEGVKKNINLVNAVREVIGYDNDLMLEAYM
                     GWNLEYSKNIIPQLTKFKPKWLEEPVIPDDIAGYAELNAIGKIPIAGGEHEFNFYGFK
                     HLLELKAVSYIQYDANRVGGITAAQKINLLAEKYNIPVVPHAGQMHNYHLTMASEN"
BASE COUNT          306 a          202 c          134 g          400 t
ORIGIN      
        1 caattttcac ttgccattgt taaatgataa ttatgcattt ggcctgcatg tggcactact
       61 ggaatattat atttttcagc aagtaaattt attttttgtg ctgcggttat tccaccaaca
      121 cgattagcat catactgaat gtaactaact gcttttaatt ccagtaaatg tttaaaacca
      181 taaaaattaa attcatgctc tccaccagct attggaattt taccaatagc gtttagttca
      241 gcataaccag ctatatcatc tgggataaca ggttcttcca accatttagg tttaaatttg
      301 gttaattgcg gtataatgtt tttagaatat tctaaattcc accccatata agcttcaagc
      361 attagatcat tatcataacc aatgacttct cttaccgcat ttacaagatt aatatttttt
      421 tttactcctt caggtccatc ttttggaccc caaccaaaac gcattttaaa cattttaaaa
      481 ccttctttta tgtacttctc agcctctttt tgaagttcat ttataggttg gctgtagagt
      541 tttgaagcat aaacaggaat tttttcctta gttctatttc caagaagttt aaatacaggt
      601 ttatttttaa ttttacccat taaatcccaa atacctagat caattgcaga tattccaatc
      661 atgcctattc ctttccttcc ccatgccatt gtatttctat acattttttc ccaaagatat
      721 tcataatcaa acgggtcttc ttctattact aaatctttta aataacagtc tattgctttc
      781 ttggtaactt gtggcgctaa tgctgcatta cctataccta cagttccatc atccgctatt
      841 acttcacaaa ctaaccattc gtggaaacga aaggatttca ttttgtctcc tttttcacga
      901 agtaaatcag taggatttgt acagaaattt tgagctggcg gaacaatcgg accagtccat
      961 ttatagattg tagtttttat atcttttatt ttagtcatgg taataaattt tcacttaatt
     1021 ttagtgctac tttaaatgat tc
//
LOCUS       DTVV01000023            2058 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1080547, whole genome shotgun sequence.
ACCESSION   DTVV01000023 DTVV01000000
VERSION     DTVV01000023.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1080547"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="EYO86_00285"
     CDS             <1..227
                     /locus_tag="EYO86_00285"
                     /inference="COORDINATES: protein motif:HMM:PF01195.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="HIC41508.1"
                     /translation="IDEAIGKNFKRLRLGIGHPGSKDLVSPYVLKKFSRKDRKFINNK
                     IELLTKYFSLIFENKSLLLTKISLEQDSGI"
     gene            217..1317
                     /gene="ychF"
                     /locus_tag="EYO86_00290"
     CDS             217..1317
                     /gene="ychF"
                     /locus_tag="EYO86_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIC41509.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTKSNKAKAANYPFATIKPNVGR
                     VAVPDKRLQQLSKIGKSEKTIPTYIDFIDIAGLVKGASTGGGLGNKFLAHIREVDAIA
                     HVVRCFEDTNITHVSSKINPIDDIDTISTELKLADLEILQNILDSLKKKSNNGEKEIK
                     TQIELISDLQERLNKNETIKNLELNKESSFIYKSLNLITTKPMLYICNVDNKSVINGN
                     YFSNQVKEKAKKELSKTVIVSAAIESQIAEYNNENDKLELLKDIGLTETTLNKVISAG
                     YDILNLITYFTCGPKETRAWTINANTRAPKAAGKIHTDFEKGFIRAETISYNDFEDLG
                     GETACREAGRLRQEGKEYIVKDGDVMHFLFNN"
     gene            complement(1314..2033)
                     /locus_tag="EYO86_00295"
     CDS             complement(1314..2033)
                     /locus_tag="EYO86_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIC41510.1"
                     /translation="MPSFCILALGNDAEMPKHEWSFGGITGTFDRASIQRGYKVYREV
                     CSGCHSMSLLYYRDLIDIGFSEEEVKAISAEYTVQDGPNDEGEMFERPARPSDRFVSP
                     FANEQEARMNNNGSYPPDLSVITKAKKHGADYIYNLLLGYKEPPEDYEIGEGMYYNEW
                     KEGHQIAMAPPLDEGYVDYDDGTENTLPQLSEDVTTFLVWSAEPELEVRKNLGIKVIL
                     FFIIFGIFIYISKKRLWRDIH"
BASE COUNT          764 a          329 c          321 g          644 t
ORIGIN      
        1 gcattgatga agcgattggc aaaaacttta aacgactaag attgggtata ggtcatcctg
       61 gttcaaaaga tttagtatcc ccttatgttt taaaaaaatt ctccagaaaa gatagaaagt
      121 ttattaataa taaaattgaa ttactcacta aatatttttc tctaattttt gaaaataaaa
      181 gtttgttatt aactaagatt tcattggaac aagatagtgg gatttaactg cggaattgtt
      241 ggacttccta atgtaggaaa atcaacacta ttcaatgctt taactaaatc aaataaagct
      301 aaagcagcta actatccttt tgcaacaata aagcctaatg taggacgtgt agctgttccg
      361 gataaaaggc tacaacaatt atcaaaaatt ggcaaaagtg aaaaaactat tcctacttac
      421 attgatttta ttgatatcgc cggtcttgtc aaaggagctt caactggagg agggctagga
      481 aataagtttc tggcacatat tcgggaagtt gatgctattg ctcacgttgt tagatgtttt
      541 gaagacacaa atattactca tgtttcatca aaaattaatc cgattgatga tattgataca
      601 atctctactg aattaaaatt agccgatctt gaaattcttc aaaatatact tgattcatta
      661 aagaaaaaat caaacaacgg tgaaaaagaa atcaaaactc aaattgagtt aatatctgat
      721 ttgcaagaga gattgaataa gaatgaaaca ataaagaatc ttgaattaaa caaagaaagc
      781 tcattcattt acaagtcatt aaatcttatt acaactaaac ctatgttgta tatttgcaat
      841 gttgataaca aatctgttat aaatggaaat tacttttcca atcaggttaa agaaaaggca
      901 aaaaaagaat taagtaaaac tgttattgtc tcagctgcaa tagagtcaca gattgctgaa
      961 tacaataatg aaaatgataa attagaatta ctaaaagata taggattaac agaaacaact
     1021 cttaataaag ttattagtgc tggatatgat atcttaaatt taattacata ttttacatgt
     1081 ggaccaaaag aaacacgtgc atggactatc aatgccaata caagagcgcc aaaagctgca
     1141 ggaaaaattc atacagattt tgaaaaagga tttattcgag cagaaacaat ttcttacaat
     1201 gattttgaag atttgggtgg tgaaacagct tgtcgtgaag caggaagatt aaggcaagaa
     1261 ggcaaagaat acatcgttaa agatggtgat gtaatgcatt ttctattcaa taattaatga
     1321 atatctctcc aaagcctttt ttttgatata taaataaata ttccaaaaat tataaaaaat
     1381 aaaattactt taatccctaa attttttctt acttctaact ccggctcagc tgaccataca
     1441 aggaaagttg taacatcttc agataattga ggaagtgtat tttctgttcc atcatcataa
     1501 tcaacataac cttcatccaa cggtggggcc attgcaattt gatgaccttc tttccattca
     1561 ttataataca ttccttcgcc aatttcataa tcttcaggcg gctctttgta tccaagtaaa
     1621 agattataaa tataatcagc tccgtgtttt tttgcttttg taataacaga tagatctgga
     1681 ggatatgaac cattattgtt cattcttgct tcctgctcat tagcaaaagg agaaacaaag
     1741 cggtctgatg gccttgctgg acgttcaaac atttctcctt catcattcgg cccatcttgg
     1801 acagtatatt cagcagaaat cgcttttact tcctcttctg aaaaaccaat atcaattaaa
     1861 tctctatagt aaagaagaga cattgaatga catcctgaac atacctctct gtaaacttta
     1921 tatcctcgct ggatagatgc tctgtcaaat gtccctgtta ttccaccaaa ggaccattca
     1981 tgtttaggca tttcagcatc attacctaag gctaagatac aaaaacttgg cattaaaaaa
     2041 agaaaagtta ataacaaa
//
LOCUS       DTVV01000024            1472 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1083058, whole genome shotgun sequence.
ACCESSION   DTVV01000024 DTVV01000000
VERSION     DTVV01000024.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1083058"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(343..1260)
                     /locus_tag="EYO86_00300"
     CDS             complement(343..1260)
                     /locus_tag="EYO86_00300"
                     /inference="COORDINATES: protein motif:HMM:PF02894.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIC41511.1"
                     /translation="MDMIITNKEIDIVDICLPPHLHFSTALKFLKAGKHVICEKPLST
                     SVNEAQKLIDVSNSMNKNLYPVFQYRYGKHILILKDLISKGKTGKPIVASSEVHWNRN
                     EEYYNVPWRGSWEGEGGGAILSHAIHSHDLLSYFFGNIKSVSANISTLVNQIETEDCA
                     SIAMTTEFGALISSSITLGAANDTTRLKLVFEKISVESGNNPYAPGEDEWIYTARDPN
                     KKNELDNLIFEAKKNILQDHNGFTSFFQEVANHLMGKKSNIIKPEDGLKSIELVAAIY
                     HAARTECKVYLPLDTNSLICKDWKPKKQI"
BASE COUNT          443 a          271 c          249 g          509 t
ORIGIN      
        1 ggtctccctg caggatggcc tcaaggcagt gctgattgga ctggcagcac accgttccat
       61 caaaactgga aaagcgatcg acctcaccag tggtgagtac agtatctcct gaataatttg
      121 tgaaaggaat ccacgatcct gactgctagg taaagctgtg ctacttttta ccccaaaatc
      181 attcggctca gtaaatcgat caccagttat gaaacaaata ttttatttta gtattttggc
      241 tatatgttga tgatttaatt tgttatcatt cacaatattt gtatttttca tatatatata
      301 tttaatttgt gtaacttaaa taatttataa taaatataaa agttatattt gtttttttgg
      361 cttccaatct ttgcagatta aactgtttgt atcgagaggt aaataaacct tgcattctgt
      421 tctagctgca tgataaatag cggctacaag ctcaatagat tttaatccat cttcgggttt
      481 gataatattt gattttttac ccatcaagtg atttgcaact tcttgaaaaa aacttgtaaa
      541 accattgtga tcctgaagaa tatttttttt agcctcaaaa attaagttat ccagttcatt
      601 ttttttgttt ggatcacgag cagtataaat ccattcatcc tcaccaggtg cgtaaggatt
      661 atttccactt tccacagata tcttttcaaa aaccaacttc aatcttgtgg tatcattagc
      721 agctcctaga gttatagatg atgaaattaa agcaccaaac tcagttgtca ttgcaataga
      781 agcacaatct tctgtttcaa tttggtttac taaagtagaa atatttgcag acacactttt
      841 tatatttcca aaaaaatatg ataggagatc gtgcgagtga atggcgtgac ttaaaattgc
      901 acctccaccc tctccctccc agcttccccg ccatggaaca ttgtaatatt cttcattacg
      961 gttccaatga acctcagatg aagcaactat tggttttcct gtttttccct tactaataag
     1021 atcttttaaa ataagaatat gttttccgta tctatattga aatactggat acaaattttt
     1081 gttcatagaa tttgaaacat cgattaattt ttgtgcctca ttaacacttg tagaaagagg
     1141 tttttcacaa ataacatgtt tgcccgcttt aaggaatttg agagcagtag aaaaatgaag
     1201 atgaggtggc aaacaaatgt ctacaatatc tatttcttta tttgttatta tcatatccat
     1261 gctgttttct atctgactac ctggagaatt tatcgcaaga tcatttgctt tttcatcatc
     1321 aatatcgcag atataactta cattaaaatc ggataattcc tttaaacttt gaagatgtgt
     1381 cgcagctctc gatcgggtcg atctcggggc tcacgtccgc ggcgatcagc aatgatgggt
     1441 ggtagtagcg acactcgtgc gtcggcagcg ga
//
LOCUS       DTVV01000025            2003 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1088525, whole genome shotgun sequence.
ACCESSION   DTVV01000025 DTVV01000000
VERSION     DTVV01000025.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1088525"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="EYO86_00305"
     CDS             <1..598
                     /locus_tag="EYO86_00305"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:PF00206.18"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIC41512.1"
                     /translation="SQQYNFITLPDELSTGSSIMPQKKNPDGAELVRAQAAQTISNLN
                     TLLVLLKGLPLTYSKDLQDDKKLTFNSYDTVHLGLQVMTELIRKTTFNTKAMKDAIES
                     SHATATDLADWLVQNLKYPFRQAYQITGKIVDYAVKKNVTLAKLSIHELQKFDKKITQ
                     DVFSVLSPIQSMKNKTSYGGTSPQGVKKSIQYAIKKYL"
     gene            598..789
                     /locus_tag="EYO86_00310"
     CDS             598..789
                     /locus_tag="EYO86_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41513.1"
                     /translation="MVHRILILIILAIVPTSCGKVGPLSLPEDKLDKSVITYPCDEAC
                     MEKFEAEKQRQQSVTLQTD"
     gene            789..1958
                     /gene="lysA"
                     /locus_tag="EYO86_00315"
     CDS             789..1958
                     /gene="lysA"
                     /locus_tag="EYO86_00315"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIC41514.1"
                     /translation="MTIFFKDNQPFVEGVCINDLINSQATPFYIYSQEAITATYHKLK
                     KALSSEIFFSVKANSNQAILKLMQSLGAGADTVSVGELERAVHAGFDPSKIIFEGVGK
                     SQQDIEFAIQHNIRLINVESLEELQLINQLGQSLNKIINISVRLNPDIDGLTLDKIST
                     GKKTDKFGITLDQLTEVIQLVQSSHHVNLKGISCHIGSQIYDIAIFEKVFTRMKKTAS
                     LILSYNIQINYVDLGGGFGVNYDDKTNELDITSINKLVQSTFDKEPYEISFEPGRYLI
                     ARAGIIITKILTRKKNGGIHYLITDAGMQTLIRPALYNIVHRVTALQNNSVHNDVYTV
                     AGPICESSDIIAKDISLPDQQMGNHLVIHDTGAYGAVMASNYNSRGFPAEILIHK"
BASE COUNT          715 a          347 c          330 g          611 t
ORIGIN      
        1 tagtcaacaa tacaacttta tcacattgcc tgatgaactt tccactggca gctcaatcat
       61 gccacagaaa aaaaaccctg atggtgctga attggtacgc gcacaagctg ctcaaacaat
      121 tagcaatctt aatactctat tagttttgct caaaggattg cctctcacgt atagtaaaga
      181 tctgcaagat gataaaaaat taacatttaa ctcttacgat actgttcatc tgggtctaca
      241 ggtaatgaca gagctcatca ggaaaacaac ttttaatact aaagcaatga aagatgctat
      301 cgaatcctct catgcgacag ctaccgattt ggctgattgg ttggtgcaaa atctcaaata
      361 tccatttcgt caagcgtatc aaattacagg aaaaatagta gactatgcag taaaaaaaaa
      421 tgttacccta gcaaaacttt ctatacatga actacaaaaa tttgataaaa aaataacgca
      481 agatgttttt tcagtgcttt cgcctattca aagtatgaaa aataaaacca gttatggtgg
      541 aacttcacct cagggtgtga aaaaatcaat acaatatgct ataaaaaaat atttataatg
      601 gtacatcgaa ttttaattct tattatacta gcaattgttc ctacctcttg tggtaaggtt
      661 gggccactaa gcctgccaga agataaattg gataaatcag ttattacgta tccgtgtgat
      721 gaagcctgca tggaaaaatt tgaagcagaa aaacaacggc agcagtcagt tactctacaa
      781 acagattaat gactattttt ttcaaagata accaaccttt tgttgaaggt gtttgtataa
      841 atgatcttat caactctcaa gctacgccct tttatattta ttcacaagaa gccatcacag
      901 caacatatca caaattaaaa aaagcccttt catcagaaat ttttttttct gttaaggcga
      961 attccaatca agcaatttta aaactaatgc agtcacttgg tgccggtgcc gatacagttt
     1021 ctgttggtga attagagcga gcagtgcatg cggggtttga tcccagcaaa attatttttg
     1081 agggagtcgg taaatcccaa caagatattg agtttgccat tcagcacaat attcgattga
     1141 tcaatgttga atcattggag gaacttcagt taatcaatca actcggtcaa tcccttaaca
     1201 aaataattaa tattagtgtg cgcttaaatc ctgacattga cggattaaca ttagataaaa
     1261 tttcaacagg aaaaaaaaca gacaaatttg gcattaccct agatcaatta acagaagtta
     1321 tccaattagt gcaatcttct catcatgtta atttaaaagg cattagttgt catataggta
     1381 gtcaaattta tgacatagca atttttgaaa aagtttttac tagaatgaag aaaactgcat
     1441 cattaatttt atcatataac attcaaatta attacgttga tttaggaggc ggttttggag
     1501 taaattatga tgacaagacc aatgaattag atatcacaag tataaataag ctcgtccagt
     1561 caacctttga taaagagcct tatgaaatct catttgagcc aggacgttat ttaattgcaa
     1621 gagcaggaat catcataact aaaattttga caagaaaaaa aaatggaggg attcattatt
     1681 tgataacgga tgcaggaatg caaacattaa tccgtcctgc cttgtataat atcgttcacc
     1741 gcgttacggc tttacaaaat aattctgtac ataatgacgt ttataccgtt gcaggaccaa
     1801 tatgtgaaag ttcagacatt attgcaaaag acatttcttt acccgatcaa caaatgggta
     1861 atcacttggt tatccatgat actggtgctt atggtgcagt tatggcttct aattataatt
     1921 ctagagggtt tcctgctgaa attttaattc ataaataaaa atattttatc attcatcatc
     1981 cagaaaaaat taccgacatt ata
//
LOCUS       DTVV01000026            5106 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1089334, whole genome shotgun sequence.
ACCESSION   DTVV01000026 DTVV01000000
VERSION     DTVV01000026.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1089334"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="EYO86_00320"
     CDS             <1..978
                     /locus_tag="EYO86_00320"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIC41515.1"
                     /translation="KNKSNLGANATLALSLAYAKALAFQKKEELFKYLSKDNNFVLPV
                     PMMNIVNGGSHADNSVDIQEFMIAPIGAPNFSESIRYGCEIFHSLKSKLKSKGLNINV
                     GDEGGFAPNINSSNEVIELIIEAVNSTGLSMNKDIVLSLDVASTEFYENSKYYLKGEN
                     KILSSDEMIEYLKALSSSYPIYSIEDGMSEDDWEGWENLTKEIGARTQLVGDDLFVTN
                     IERLQEGINRKIANSILVKVNQIGTLSETLDAINLAKKNGYTNVISHRSGETEDTTIA
                     DLAVATAAGQIKTGSLSRTDRTSKYNQLLRIEEILGNEAKFAGRQIIRY"
     gene            1023..1334
                     /locus_tag="EYO86_00325"
     CDS             1023..1334
                     /locus_tag="EYO86_00325"
                     /inference="COORDINATES: protein motif:HMM:PF04977.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41516.1"
                     /translation="MNKSLALKNKLYLFFSLLISFFIFLYLFYFLLNGERGIVSYYKI
                     RNQNIQHHLTLSALQKKNSLLTDRIKRLQTNTIDLDFLDEQIRQKTGYVSENEVLIIF
                     E"
     gene            1391..2356
                     /gene="pdhA"
                     /locus_tag="EYO86_00330"
     CDS             1391..2356
                     /gene="pdhA"
                     /locus_tag="EYO86_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIC41517.1"
                     /translation="MKKKLLKKDYIKIYTYMLKIRRFEERASQLYGMGHIGGFCHLYI
                     GQEAVVVGVLMTIKNNDSVVTSYRDHGHMIARGLEPKRIMAELTGKSNGYSGGKGGSM
                     HMFSKENNFYGGHGIVGAQVPIGTGIAFTLKYKNQKNICRTYIGDGAMNNGQVFEAFN
                     LAALWSLPVLYIIENNMYGMGTAVNRAAAGSDLYKRGESFGINGTIVDGMNVFQVIDA
                     AQKAGDFVREGNGPYILEVQTYRYRGHSMSDPAKYRSKEELENYKLNDPLEIASQEII
                     KKKIASKKDLENINKKIIKEIKDAADYALDSPFPADEDLYTNVYL"
     gene            2362..3723
                     /locus_tag="EYO86_00335"
     CDS             2362..3723
                     /locus_tag="EYO86_00335"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HIC41518.1"
                     /translation="MSVKILMPSLSPTMTEGNLTKWLVKEGDLIKSGDVIAEIETDKA
                     TMEVETVDEGVIEKLLYKEGEQSIPVNSTIAILSGDNNEEEENKDLPEQPKDNEDSSI
                     STNNIVQKSSLKEKKQNIDTSLDENLITMRQAIRDAMAEEMRKNESVFVIGEEVGEYQ
                     GAYKVTQGLLEEFGPKRIIDTPISEQGFTGLAIGSAFKGLRPIVEFMTFNFSMQAIDQ
                     IINSAAKTLYMSGGQINCPIVFRGPNGAASQVAAQHSQDFTSWYAHCPGLKVVVPSNA
                     YNAKGLLKSAIDDDNPVIFLENEILYGYKAEVPADTNFEIPLGVANIALEGSDATIVT
                     YSIMVSKSLEAAKLLKYKHDINVEVIDLQTIRPLDKVTIIESVKKTNRLVTVEESWPF
                     ASIGSEIVNIVQNEAFDFLDSPIKKINSADVPMPYSSVLEKKYLPQVEDIILAIEEVC
                     YIQ"
     gene            3729..4940
                     /locus_tag="EYO86_00340"
     CDS             3729..4940
                     /locus_tag="EYO86_00340"
                     /inference="COORDINATES: protein motif:HMM:PF00198.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIC41519.1"
                     /translation="MTVKIPMPALSPTMTEGTLTKWLIKVGDQVKAGDVLAEIETDKA
                     TMEVEAVDEGKVAKLIVEEGTSNIPVNSTIAILDGDDKDQNEGDQDQTKDTVGKIKKK
                     EILDNNVVKKSSFEKKLGKKIIASPYAKSLAKKDKLTLDNIIGSGPGGRVIKRDLKNQ
                     KTSQSSIKTSQENISAPSSMRKIIADRTVKSKQTVPHFYLTIESKVDKLLQLRKKINE
                     NFGTKISINDMLVKALALAQMENPLTNVSWVEGQVIKYSSVDVSIAVALKEGLITPIV
                     KNADKKGLIEISKEIKELVNKAKTGKLQPDEYTGGTISISNLGMFGVMEFSAIINPPQ
                     SSILAVGTIKKIPGVIEEEIRPISVLKSTLSADHRVLDGAVAGKLLKDFHEIIEDPFD
                     LWLKSSDMEII"
     gene            4940..>5106
                     /locus_tag="EYO86_00345"
     CDS             4940..>5106
                     /locus_tag="EYO86_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIC41520.1"
                     /translation="MTIRHPEKINRQTNPIPKKPSWIRVKAPTSNLFKKTRDIIKKNN
                     LITVCEEAACP"
BASE COUNT         1930 a          675 c          945 g         1556 t
ORIGIN      
        1 aaaaataaat caaatttagg ggctaatgca actctagctc ttagtctagc ttatgctaaa
       61 gcgctagcat ttcaaaaaaa agaagaactt tttaaatatc tgtctaaaga taacaatttt
      121 gttttacctg tgccgatgat gaacatagta aatggtgggt cacatgcaga taacagtgta
      181 gatatacaag aatttatgat tgctcctata ggagctccta attttagtga gtcgattaga
      241 tatggttgtg aaatatttca ttctttaaaa tcaaaattaa aatctaaagg attaaatatt
      301 aatgtagggg atgaaggtgg ttttgctcca aatattaatt cctccaatga agtgattgaa
      361 ttaataattg aagcagtaaa ttctacagga ttatcaatga ataaagatat tgtgttatct
      421 ttagatgttg cctctactga attctatgaa aattctaaat attatcttaa aggagaaaat
      481 aagattttat cttctgatga aatgatagaa tacttaaaag ctttaagttc ttcataccca
      541 atttattcta ttgaagatgg tatgtcagag gatgattggg aaggttggga aaatcttact
      601 aaagaaattg gagccagaac gcaattagta ggagatgatt tatttgttac aaatatagaa
      661 agattgcaag aagggattaa tagaaaaatt gctaactcaa ttttagtaaa agttaatcag
      721 attggaactc tgtcagaaac tttagatgca ataaatttag ccaaaaaaaa tggttataca
      781 aatgtaattt cacaccgctc tggtgaaaca gaagatacca ctattgccga tttagcagtg
      841 gcaactgcag ctggtcagat aaaaactggt tctttatcaa gaacagatag gacttcaaaa
      901 tataatcaat tacttcgaat tgaagagatt cttggaaatg aggcaaaatt tgcaggtagg
      961 caaatcatta gatattgaca aaaacccatg tattctgatt taatttaaat tattatatca
     1021 aaatgaataa atccttggca cttaaaaata aactatattt atttttttcg ttattaattt
     1081 ctttttttat ttttttatat ttattttatt ttttattaaa tggagagaga gggatagttt
     1141 cttattataa aattagaaat caaaatatcc aacaccactt gacattgtca gcacttcaaa
     1201 agaaaaattc tttattaact gatagaatta aaagattgca gacaaatact atagatttgg
     1261 attttcttga tgagcaaatt cgtcaaaaaa caggctatgt tagtgaaaac gaagttttaa
     1321 ttatatttga ataataattt attgacaaat atttacaaat ttattattta aaatttacaa
     1381 aaatttacaa atgaagaaaa aactattaaa aaaagattat attaaaattt acacctatat
     1441 gcttaaaatc agaaggtttg aggagcgtgc atcacaattg tatggaatgg gtcatatagg
     1501 cggtttttgc catttatata taggtcagga agccgttgtt gtgggtgttt taatgaccat
     1561 aaaaaataat gatagtgtag taacatctta tagagatcat ggacatatga ttgcaagggg
     1621 tttggagcca aaaagaatta tggctgaatt aactggcaaa agtaatggtt attcgggtgg
     1681 aaaagggggc tctatgcata tgttttcaaa agaaaataat ttttatggcg gtcatgggat
     1741 tgtcggagca caagtaccta ttggcacagg aattgcattt actcttaaat ataaaaatca
     1801 aaaaaatatt tgtagaactt atatcggtga tggagcaatg aataatggcc aagtatttga
     1861 ggcatttaat ctagcggctt tatggtcttt gcctgtttta tatattattg aaaataatat
     1921 gtatggaatg ggtactgcgg ttaatagggc agctgctgga agcgacctat ataaaagagg
     1981 agaatctttt ggaattaatg gcactattgt tgacggaatg aatgtttttc aagttattga
     2041 cgcagcccaa aaagcagggg actttgtgag agaaggcaac ggtccatata ttctagaagt
     2101 gcaaacttat aggtacagag gccattcaat gtcagatcct gcaaaatata gatccaaaga
     2161 agaactagaa aactataaat taaacgatcc tttggagata gcttctcagg aaattattaa
     2221 aaaaaaaata gcttcaaaaa aagatttaga aaatattaat aaaaaaatca taaaggaaat
     2281 aaaagatgca gctgattatg ctcttgacag cccctttcct gcagatgaag atttgtacac
     2341 aaacgtatat ttataaaaaa aatgtcagtt aaaatcttga tgccatcact ttctccgaca
     2401 atgacagaag gaaatttaac taagtggctt gttaaggaag gtgacttaat taaatctggt
     2461 gatgttatag ccgaaattga aactgataaa gctacgatgg aagtagagac tgtagatgaa
     2521 ggagttattg aaaagttatt atataaggaa ggagagcaat ccataccagt taattctaca
     2581 atagcaattt tgtctggtga caataatgaa gaagaagaga acaaagactt accagagcaa
     2641 ccaaaagaca atgaagactc tagtatttct accaacaata tagttcagaa aagtagcttg
     2701 aaagaaaaaa aacaaaatat agatacttct ttagatgaaa atttaattac aatgcgacaa
     2761 gccattagag atgcaatggc ggaggaaatg agaaaaaatg aatctgtttt tgttattgga
     2821 gaagaagtcg gagagtatca aggagcatac aaagtaaccc aaggcttatt agaggagttt
     2881 ggacctaaaa gaattattga taccccaatt tcagaacaag gatttactgg tttagctatc
     2941 ggaagtgctt ttaaaggttt gagaccaata gttgaattta tgacatttaa tttttctatg
     3001 caggccattg atcagattat taattctgct gccaagactc tctatatgtc tggaggtcaa
     3061 attaattgcc caatagtatt tagagggcca aacggtgcag catcgcaagt tgcagctcaa
     3121 catagtcaag attttacttc atggtatgca cattgccctg gcttaaaggt tgtagtccct
     3181 tctaatgcgt ataatgcaaa aggattgctt aagtcagcca tagatgatga taaccctgtt
     3241 atttttttag agaatgagat tttatatgga tataaggctg aagtacctgc tgacactaat
     3301 tttgaaattc cacttggagt tgccaatatt gctttagagg gtagtgatgc aacaatcgtt
     3361 acatattcaa tcatggtttc taaatcattg gaggcagcta aattattaaa atataagcac
     3421 gatattaacg ttgaggtaat tgatttacag acaatacgcc ctctagataa agtaacaatt
     3481 attgagtctg ttaaaaaaac caacagacta gtgacagtcg aggaatcctg gccttttgca
     3541 agtataggat cagaaattgt taacattgtt caaaatgaag cttttgattt tttagattct
     3601 cctattaaaa aaatcaatag tgctgatgtg ccaatgcctt actctagcgt cttagaaaaa
     3661 aaatatttac ctcaagtaga agacattatt cttgctattg aagaagtctg ctatatccaa
     3721 taaattttat gactgtaaag attcccatgc ctgctttgtc tcctacgatg acggaaggca
     3781 ctttaacaaa atggttaatc aaagtaggtg atcaagttaa agctggtgat gtattagcag
     3841 aaattgaaac tgataaagct acgatggaag tagaggctgt agatgaagga aaggtagcaa
     3901 aattaattgt tgaagaaggt acttcgaata ttccagttaa ttctaccata gcaatattgg
     3961 atggagatga caaagatcaa aacgaaggag atcaagacca aactaaagat acagttggaa
     4021 aaattaaaaa aaaagaaatt ttagataata atgttgtcaa aaaatcttct tttgaaaaaa
     4081 aattaggtaa aaaaattata gcatctcctt atgctaaaag tttagcaaaa aaagataaat
     4141 taacactaga taatatcatt ggttctggac ccggaggaag agtaatcaaa agggatttaa
     4201 aaaatcaaaa aactagtcag tcttctataa agacttccca agaaaatatt tctgcaccaa
     4261 gttctatgag aaaaattatt gctgatagaa ctgtaaaatc aaaacaaact gttcctcatt
     4321 tttatttaac tatagaatca aaagttgata aactactgca attaagaaaa aaaattaatg
     4381 aaaactttgg tactaaaatt tcaattaatg atatgctagt aaaggcacta gctctggctc
     4441 agatggaaaa cccattaaca aatgtatcat gggtagaagg tcaggttata aaatatagct
     4501 ctgttgatgt gtctattgcc gttgctttaa aagagggatt aattacacct atagtgaaga
     4561 atgcagataa aaaaggacta attgaaatat caaaggaaat caaagagtta gtcaataaag
     4621 ctaagactgg taagttgcag cctgatgaat acactggagg aacaatttcc atttccaatc
     4681 ttggaatgtt tggtgttatg gaattttcag ctattattaa ccctcctcaa tcttctattc
     4741 tagcagttgg aacgattaaa aaaattccag gtgttattga ggaagagatt aggcctatta
     4801 gtgtgttaaa atcaacatta tcagcagatc atagagttct agatggagct gttgcaggta
     4861 aattgctgaa ggattttcat gaaataatag aagatccctt tgatttatgg ttaaagagca
     4921 gtgatatgga aataatttga tgacaattcg acatcctgaa aaaatcaata gacaaactaa
     4981 tcctataccg aaaaagcctt catggataag agtaaaggca cccacttcta atttattcaa
     5041 gaaaacaaga gatattataa aaaaaaataa tttaattact gtttgtgagg aggcggcttg
     5101 cccaaa
//
LOCUS       DTVV01000027            3525 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1097175, whole genome shotgun sequence.
ACCESSION   DTVV01000027 DTVV01000000
VERSION     DTVV01000027.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1097175"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            26..769
                     /locus_tag="EYO86_00350"
     CDS             26..769
                     /locus_tag="EYO86_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIC41521.1"
                     /translation="MSASGENHNPLEQFEIKPFVHFEVGSVDLAFTNSSLAMVITIAV
                     ITLFLTLSVNTRSIIPSRLQLISELSYGFIAQLLKDTVGEQGRKYFPFVFTIFMFVLI
                     GNMVGMLPYSFTFTSHIIVTFALAAVVFVGVTILGFAKHGMNFFSFFVIPGLPWYMLP
                     LLIPIEVISYLSRPISLSVRLFANMLAGHTLIKVFAMFVITMPFYSGVLPLAFIVALT
                     GLEILIAFLQAYVFAILTCLYINDALHLH"
     gene            786..1010
                     /gene="atpE"
                     /locus_tag="EYO86_00355"
     CDS             786..1010
                     /gene="atpE"
                     /locus_tag="EYO86_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020021630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIC41522.1"
                     /translation="MEVEAAKLIGAGLAVIGVVGSGIGIGSIFSSFIEAVGRNPAARS
                     EVFTMTMLGFALVEAIALFALVIALVILFT"
     gene            1017..1526
                     /locus_tag="EYO86_00360"
     CDS             1017..1526
                     /locus_tag="EYO86_00360"
                     /inference="COORDINATES: protein motif:HMM:PF00430.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41523.1"
                     /translation="MNFIVPQLEQTEFFISQLFWLVVTFTFLFIFLWRISLPRISSVL
                     EKRESKIDDDITSAKQLQAEAEEIQKQIDQQLRNARLETSELIKTASTKFQNHTTKEL
                     HQLDNNLSNTIEESATTIEKNIKDSLKQIHDQTYLIAKLTLSKISNIPVNDNEIKDTV
                     DQLQPKVIN"
     gene            1529..2014
                     /locus_tag="EYO86_00365"
     CDS             1529..2014
                     /locus_tag="EYO86_00365"
                     /inference="COORDINATES: protein motif:HMM:PF05405.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41524.1"
                     /translation="MFVDPQFWVAIAFIIFIVAVFNPIRKMLGTTLNSKIQDIKNSIE
                     EAENIKNETQNTLSDLKKRQNDVQIEIENIHKDAKEKIQILESQAEEKLKEKIDKRNL
                     LATAKIEQMTRDANAAIQRHISRTAIEAAVTILKKKLDQNEKQNLINRSIKELSSVFK
                     N"
     gene            complement(2011..2532)
                     /locus_tag="EYO86_00370"
     CDS             complement(2011..2532)
                     /locus_tag="EYO86_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41525.1"
                     /translation="MKNIIFFISLLMLISCGSNPLSTSKKEFIKCPTVLFAAEHKKYL
                     RINASSISVDDVAYKAEINNYNFSKGCFINDNNFTASLSILFIVKPLIKEQNIITLPF
                     YLAILDKEKKLRDIQYYYTEGNFNKNLETETKEFVETDLSKTITINIPDVDGMITIVV
                     GFMLDKQQLEILN"
     gene            2599..>3525
                     /gene="ispH"
                     /locus_tag="EYO86_00375"
     CDS             2599..>3525
                     /gene="ispH"
                     /locus_tag="EYO86_00375"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017971113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIC41526.1"
                     /translation="MTSDVINIYLAGPRGFCAGVERAVLMVEEAIKKYGGPIYVRHEI
                     VHNKYVVEDLKVKGAVFIDELEEIEDRTRPVIFSAHGVPKSVPKEAKNLNLLFFDATC
                     PLVSKIHREVENFERLHVPIILVGHRNHPEVIGTMGQIQTPIYLIEKEEDLNNLPIKR
                     DQKIAYVTQTTLSVDDTESIIQSIKKKFTNVIEPRKNDICYATSNRQNAIKKIASCCD
                     LFIVIGSHNSSNSLRLVEVAKQYGAKESILIENLEIFNLSKFKNLNNVGLTASASAPE
                     ILVQNFIQIIQKKYKINLHESDFAPENVNFKIP"
BASE COUNT         1226 a          582 c          572 g         1145 t
ORIGIN      
        1 aattcaaata ttacacagat ataaaatgtc agcttcagga gaaaatcaca atccgcttga
       61 acagtttgag attaaacctt ttgtgcactt tgaggtgggt agtgtggatc ttgcctttac
      121 caattcttct cttgccatgg tgattactat agcagttatc actctttttt taactctttc
      181 tgttaatacg cgatcaatta ttccttcccg tttacagtta atctccgagc tcagttatgg
      241 ttttattgca caattactaa aagacacagt tggtgagcaa gggagaaaat attttccctt
      301 tgtttttact atttttatgt ttgtattaat tggcaatatg gtgggtatgc tcccctatag
      361 cttcacgttt acaagccata tcattgttac atttgcccta gccgcggttg tctttgttgg
      421 tgtcactatt ctaggtttcg ccaagcacgg tatgaatttt ttttcttttt ttgttattcc
      481 cggtttgccc tggtatatgt tacctctatt aatccctatt gaggtaattt cttatctatc
      541 gcgaccaatc agtttgtctg tccgactttt tgccaacatg ttggcaggtc atacactcat
      601 aaaagtcttt gccatgtttg taataacaat gcctttttat agtggtgttt tacccttagc
      661 attcatagtt gcattaactg gtttagaaat attaatcgca tttttgcagg cgtatgtatt
      721 tgctatctta acttgcttat acataaatga tgcattacat ttacattaac aactaaggag
      781 gatttatgga agtagaagca gcaaaattaa taggagcggg actagccgtt atcggtgtag
      841 taggttccgg tattggaatt ggaagtattt tttcttcatt tattgaagcc gttggtcgta
      901 acccagctgc acgtagtgaa gtttttacaa tgacaatgtt agggtttgcg cttgtagaag
      961 caattgcact attcgcactt gttatcgctc tggttatatt atttacgtaa taaaaaataa
     1021 attttatagt gccacagtta gaacaaacag aattttttat ctctcagctt ttttggttgg
     1081 ttgttacatt cactttctta tttatattcc tatggcgaat atcattacct cgaatcagtt
     1141 ctgtgttgga aaaaagagaa agtaaaattg atgatgatat tacttcagca aaacaacttc
     1201 aggctgaggc tgaggaaata cagaaacaaa ttgatcaaca attgcgtaat gcacgattag
     1261 aaacatctga gttaattaaa acagcttcaa ctaaatttca aaatcataca actaaggaat
     1321 tacatcagtt agataacaac ttaagtaata caatagaaga atccgcaact acaattgaaa
     1381 aaaatataaa agattcatta aagcagatac atgatcaaac ttacttgatt gctaagctta
     1441 cactctcaaa aatttctaat atcccagtaa atgataatga aattaaggat actgtcgatc
     1501 aattacaacc aaaagtaata aactaattat gtttgttgat ccgcagtttt gggttgcaat
     1561 agcctttatt attttcatag ttgctgtttt taatcctatt cgaaaaatgc ttggtacaac
     1621 ccttaatagt aaaattcagg atataaaaaa tagtattgag gaggcagaaa atattaagaa
     1681 tgaaacgcag aatactctca gtgatttaaa aaaacgccaa aatgatgttc aaatagaaat
     1741 tgaaaatatt cacaaagatg ccaaagaaaa aatacagatt ttggaatcgc aagctgagga
     1801 aaaactaaaa gaaaaaattg ataaacgcaa cttattggcc acggctaaaa ttgaacaaat
     1861 gacaagagat gccaatgccg caattcaacg acatatttca cgaacagcta ttgaagcagc
     1921 cgtaactatt ctaaaaaaga aattagacca aaacgaaaaa caaaatctta ttaatcgatc
     1981 aataaaagaa ttaagctcag tttttaaaaa ttaatttaga atttccaatt gttgcttatc
     2041 caacataaat ccaacaacaa ttgtaatcat tccgtctacg tctggtatat taattgttat
     2101 cgtcttgcta agatccgttt cgacaaattc cttagtttct gtctccaaat ttttgttaaa
     2161 attaccttct gtgtaataat attgaatatc acgtaatttt ttttccttat caagaatagc
     2221 aagataaaat ggaagggtaa taatattttg ttcctttatc agaggcttga caataaatag
     2281 gatcgataag gaggcagtga aattattatc atttataaaa caacccttgg aaaaattata
     2341 attgtttatt tccgctttgt atgcaacatc atctacgcta atagaggagg cattaattct
     2401 caaatatttt ttatgttcag ctgcaaataa gactgtgggg catttaatga actctttttt
     2461 tgatgttgac agagggttgg atccacaact aatcaacatc aaaagcgaaa taaaaaaaat
     2521 aatatttttc actatacgac ttaattaata taaatatcaa aagaagtata tagacacaaa
     2581 tctattaaac aaataattat gacatcggat gtaatcaata tttatcttgc cggtcccaga
     2641 ggattttgcg caggagtaga acgtgcggta ttgatggtgg aagaagccat taaaaaatat
     2701 ggaggtccta tttacgtacg tcacgaaatt gttcataaca aatacgtagt agaagattta
     2761 aaagtaaaag gagctgtttt tattgatgag ttagaagaaa ttgaagatcg tacacggcct
     2821 gttattttct ctgcacacgg agttccaaaa tcagtaccaa aagaagcaaa aaatttaaac
     2881 ctgctcttct ttgatgccac ttgtccactt gtcagtaaaa tacaccgtga agtggaaaat
     2941 tttgagcggt tacacgttcc catcattctc gttgggcacc gcaatcaccc tgaagttata
     3001 ggtacaatgg gacaaataca aacacccatc tacttaattg aaaaagaaga agatctgaat
     3061 aatcttccta tcaaaagaga tcaaaaaatt gcctacgtca ctcaaacaac tctttctgtg
     3121 gatgacactg aatccattat acaatctatt aaaaaaaaat ttacaaatgt aatcgaacct
     3181 cgtaaaaatg atatttgtta tgcaacatcc aaccgtcaaa atgcaataaa aaaaattgcc
     3241 tcgtgttgtg atcttttcat tgttattggt tcgcataatt catcgaactc gcttcgcctt
     3301 gtagaagtag caaaacaata cggcgccaaa gaaagtatat taatagaaaa tttagaaata
     3361 tttaacctaa gcaagttcaa aaatcttaat aatgtaggtt taactgctag tgcttcggca
     3421 cctgaaatat tagtacaaaa ttttattcaa atcatacaaa agaagtataa aataaatttg
     3481 catgagtctg attttgctcc tgaaaatgtc aactttaaaa tccct
//
LOCUS       DTVV01000028            1027 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1099565, whole genome shotgun sequence.
ACCESSION   DTVV01000028 DTVV01000000
VERSION     DTVV01000028.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1099565"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="EYO86_00380"
     CDS             complement(<1..288)
                     /locus_tag="EYO86_00380"
                     /inference="COORDINATES: protein motif:HMM:PF06245.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIC41527.1"
                     /translation="MHFINPFKGLRPTEEKASSVSIPSTDHLSQKVITDHKKNNPWSY
                     LNIFNPDDQNSKSQNEIDAKAKEQFELMKSNSVLMKDNINSFYIYKISTKNH"
     gene            complement(314..>1027)
                     /locus_tag="EYO86_00385"
     CDS             complement(314..>1027)
                     /locus_tag="EYO86_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIC41528.1"
                     /translation="MAAQKDTTKYSLGSVLNHVLMHQTVVGNEAIMQMEIAGDYPDIM
                     IGCTGGGSNFSGLVFPFLGKKFRDKKDIKVIACEPKSCPSLTKGKYAYDFGDTGNLTP
                     LVKMHTLGSSFIPPATHSGGLRYHGMAPMVSHLKEIGAIEAKAFGQKECFEAGVKFAN
                     VEGIVPAPEATHAVKGVIDAALECKKNGKSKTILFNLCGHGHFDMQAYGDYFDNKLEE
                     DAYNEAEVNKALQSLPKIA"
BASE COUNT          328 a          187 c          142 g          370 t
ORIGIN      
        1 atgatttttt gttgaaattt tataaatata aaaagaatta atattatctt tcattaatac
       61 cgaattgctc ttcatcaatt caaattgttc ctttgcttta gcatctattt cattttgaga
      121 tttcgaattt tgatcatctg gattaaaaat atttaaataa ctccacgggt tatttttttt
      181 atgatctgta attacttttt gagataaatg atctgtgcta gggatagata cagatgaagc
      241 cttttcttca gttggtctta aacctttaaa tggatttata aaatgcataa caaactataa
      301 ataaaaaaac ttactatgca atcttaggta aggattgtag tgctttattt acctcagctt
      361 cattataagc atcttcttca agtttattat caaaataatc accgtaagct tgcatgtcaa
      421 aatgaccgtg accacataga ttaaaaagaa tagtttttga tttgccattc tttttacatt
      481 ctaaagcagc atcaataaca cctttaacag catgtgttgc ttctggtgct ggaacaatgc
      541 cttcaacatt tgcaaacttc acgccagctt caaagcattc cttttgtcca aaagcttttg
      601 cctcaatagc tcctatttct tttaaatgac taaccattgg agccattccg tggtacctca
      661 aacctcctga atgtgttgct ggaggtataa aagaagaacc taaagtatgc attttaacca
      721 aaggtgtaag attacccgtg tctccaaaat cataagcata tttacctttt gttaaagaag
      781 ggcaagattt cggttcacat gcaataactt ttatatcttt tttatctcta aatttttttc
      841 caagaaaagg aaaaactaag cctgaaaaat tacttcctcc accggtacaa ccaatcatta
      901 tatctggata gtcacctgct atttccattt gcattatcgc ttcattaccc acaacagttt
      961 gatgcattaa tacgtgattt aaaacacttc ctagagaata tttagtggta tctttttgcg
     1021 cagccat
//
LOCUS       DTVV01000029            5286 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1107131, whole genome shotgun sequence.
ACCESSION   DTVV01000029 DTVV01000000
VERSION     DTVV01000029.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1107131"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(62..673)
                     /locus_tag="EYO86_00390"
     CDS             complement(62..673)
                     /locus_tag="EYO86_00390"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIC41529.1"
                     /translation="MIIYTLSFYLFSTIAVLSALMVISSKNSVHSVLFLILSFVNAAG
                     LFVLLGAEFLAMILLVVYVGAVAVLFLFVVMMLDINYIKLREGFLQYLPFGVLLGIVL
                     IVELGIAFFTETFSEMSLVEYAQMPEITITENTKLLGFVLYTKYFYLFQISGIILLVA
                     MIGSITLTLRQREISKKQIISDQNNADASKAIIKKKIKLREGI"
     gene            complement(677..1165)
                     /gene="nuoI"
                     /locus_tag="EYO86_00395"
     CDS             complement(677..1165)
                     /gene="nuoI"
                     /locus_tag="EYO86_00395"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIC41530.1"
                     /translation="MFSVVKAIKSFALFELFSGMLLTFKYMFKSKVTINYPHEKSPLS
                     PRFRGELALRRYPNGEERCIACKLCEAVCPAQAITIEAEPRSDGSRRTTRYDIDMSKC
                     IYCGLCEESCPVDAIVEGPNYEFATETREELMYNKAKLLDNGDKWEHEIAQNLKADSP
                     YR"
     gene            complement(1167..2165)
                     /gene="nuoH"
                     /locus_tag="EYO86_00400"
     CDS             complement(1167..2165)
                     /gene="nuoH"
                     /locus_tag="EYO86_00400"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIC41531.1"
                     /translation="MNDIIAPGSIIIAQIMAVIVPVLVSVAFLVYAERKVLALIQLRR
                     GPNIVGPFGILQSFADALKLLTKENIIPSGSNKIVFILAPIITMVLSLAAWAVIPFAP
                     GWVVSDINVGVMYLFAVSSLSIYGVIMAGWASNSKYPFLGALRSAAQMVSYEVSIGFV
                     IITVLLCVGSLNLTKIVEAQTTVWFAIPLLPMFIVFFISALAETNRLPFDLPEDEATL
                     VAGFFTEYSSASFALFFLAEYASMILMSAMTVILFLGGWLPPFDIFPFNVVPGIIWFT
                     AKIIFVLFLFIWVRGTFPRYRYDQLMRLGWKIFLPLSLFWVVVTASFLTIFDMVPG"
     gene            complement(2187..4229)
                     /locus_tag="EYO86_00405"
     CDS             complement(2187..4229)
                     /locus_tag="EYO86_00405"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIC41532.1"
                     /translation="MPKITIDNREIEFENGMTVMQACELANVEIPRFCYHDKLSIAGN
                     CRMCLVEMEKSPKPIASCAMPAGEGMVIKTNTDLVKSARRGVMEFLLINHPLDCPICD
                     QGGECDLQDQALHYGFDKSRYEENKRAVNNKHMGPLVGTIMTRCIHCTRCVRFSTEVA
                     GVDDLGMLGRGENAEITTYLEKTIKSELSGNVIDLCPVGALTSKPYEFKARPWELKKT
                     ETIDVFDAMGSSIRIDSLGKTVLRVLPRLNEEINEEWINDKSRFAIDGLTKQRLDKPF
                     MRKNSKFQIVDWNTALSIIVTEINNRNKNKTLALSGKLTDIETLYSAKSFLNSIGSSS
                     YDCRYDNAQFIANQRDSYIFNSSIQKIDDADAILIIGSNPRWEATVLNARIRKAYIHN
                     NCKIGLIGKAIDLTYTYKHLSNNIDYLNDILNDQSDFCTVLKKAKKPLIIIGTSAINF
                     SDGKDILKVCAEIAKKYQITNHNFNGLNILQQDISRVGALDIGFYNNFFDLDYNNSLK
                     QHIQNYKPVVFLLGLDEIDLSILQDSFVVYLGHHGDATANIADIILPIPAFPEKTSTF
                     VNMEGRVMQTTKSYNPIGESKEEWKIFRALSDQFENKLNFNNLNELRQEIITKFIFLK
                     EINSLPSVLLSKFGLPVKIKNRELEYNIKNFYMTDSISRSSVTMAKCSKEILDKVA"
     gene            complement(4229..>5286)
                     /gene="nuoF"
                     /locus_tag="EYO86_00410"
     CDS             complement(4229..>5286)
                     /gene="nuoF"
                     /locus_tag="EYO86_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIC41533.1"
                     /translation="PHYLVINADESEPGTCKDREILRNDPHLLLEGCLVAGFAMQAHA
                     CYIYIRGEYYNESSNLQKAIDEAYVASLIGKNACGSKWDFDIFLHRGAGAYICGEETA
                     LLESLEGKKGQPRLKPPFPAGAGLYGCPTTVTNVETVAVVPTILRRGAQWFAGIGNPK
                     NTGTKIFSISGHVNNPCNVEEEMGIPLKELIEKHAGGVIGGWDNLLAVIPGGASVSLI
                     PKSICDNIKMDFDSLQGVQSGLGTAAVIVMNKTTDIVEAITRLSYFYKHESCGQCTPC
                     REGTGWMFRIMKKMIDGNVHHEDIDKLLDVTKQVEGHTICALGDAAAWPIQGLMRHFR
                     PEIEKRIEENQQRKAVA"
BASE COUNT         1761 a         1013 c          752 g         1760 t
ORIGIN      
        1 aaaacgccaa ttgtaaataa aattgctcca agaaaaagga aatgatctat agttatactt
       61 gtcatattcc ttctcttaat ttaatttttt tctttataat agcttttgaa gcgtcggcat
      121 tattttgatc cgaaatgatt tgcttcttcg atatttctct ttgccttaag gttaatgtaa
      181 tggagccaat catcgcaacc aataatatta tccctgatat ttgaaaaaga taaaaatact
      241 tagtatataa aacaaagccc aataatttgg tattttcagt aattgttatc tcaggcattt
      301 gcgcatattc aacgaggctc atttcagaaa atgtctcagt aaaaaatgca attccaagtt
      361 caacaatcaa aactattcca agcaaaactc caaagggaag atattgtaaa aaaccctcac
      421 gtaattttat ataattaatg tctaacatca taactacaaa taaaaataaa accgcaacag
      481 cgcctacata aacaacaaga agtatcattg ccaaaaactc agctcctaac agaacaaata
      541 gtcctgcagc attaacaaaa cttaaaatta aaaacaaaac agagtggacg gagttttttg
      601 aactgataac cattagtgca gacaatactg caattgttga aaacaaataa aatgaaagag
      661 tataaataat catatactaa cggtaaggcg aatcagcttt caaattttgg gcaatttcat
      721 gttcccactt atctccgtta tcaagaagtt tagctttatt gtacattagt tcttctcttg
      781 tctctgtagc aaattcatag ttaggtcctt ctacgatcgc atcaacaggg caggactctt
      841 cgcacaaacc acagtaaata catttactca tatcgatgtc atatcttgtt gttctacgac
      901 tgccatcgga tcttggctct gcctcaattg taattgcttg tgcagggcaa actgcttcac
      961 agagtttaca agctatgcat ctttcctctc cgtttggata tcttcttaaa gcaagctctc
     1021 ctctaaaacg agggctaaga ggactctttt catgaggata attaatcgtc acctttgatt
     1081 taaacatata tttaaatgtt aaaagcattc ctgaaaaaag ttcaaaaaga gcaaaagatt
     1141 ttattgcctt gactactgaa aacattttaa ccaggcacca tgtcaaaaat tgttaaaaaa
     1201 ctagcagtta caactaccca aaacaatgat agcggcaaaa aaattttcca tcccaatctc
     1261 attaattggt catatctata acgaggaaaa gttcctcgaa cccaaataaa taaaaaaagt
     1321 acaaaaataa tttttgcagt aaaccaaatt atccctggaa caacgttaaa agggaaaata
     1381 tcaaaaggtg gtaaccaccc tcctaaaaat aaaataactg tcattgcact cattaaaatc
     1441 atgcttgcat attctgctaa aaaaaataga gcaaacgatg cggaggaata ttcagtaaaa
     1501 aatcctgcta ctaaagttgc ctcatcttct ggcaaatcaa aaggcaatct atttgtttcc
     1561 gctaatgcag aaataaaaaa aactataaac atcggcaata aaggaattgc aaaccaaact
     1621 gttgtttgtg cttctactat ttttgttaaa tttaatgaac ctacacacaa aagaacagta
     1681 ataataacaa atcctataga aacttcataa gaaaccattt gagcagcaga acgaagggca
     1741 cctaaaaaag gatatttgga attactagcc catccagcca taattactcc atagatactt
     1801 aaagatgaga ctgcaaataa atacataaca ccaacattta tatctgagac aacccaacct
     1861 ggtgcaaatg gaatgacagc ccaagcagct aaacttaaaa ccattgtaat tataggggca
     1921 agaataaaaa caattttatt tgacccgctt ggtataatat tttcctttgt tagaagtttt
     1981 aatgcatcag caaaactttg aagtatgcca aaaggtccaa caatatttgg acctcttctt
     2041 aattgtatta atgctaaaac ttttctttct gcatacacaa ggaatgccac agaaaccaaa
     2101 acagggacta ttactgccat aatttgcgca ataattattg aacctggagc tatgatgtca
     2161 ttcattaaag aaaaatctga ggtcatttaa gctaccttat ctaatatttc ttttgagcat
     2221 ttagccattg taacggaaga tctactaatg gaatctgtca tataaaaatt tttaatatta
     2281 tattctaatt ctctattttt gattttaact ggtaatccaa attttgatag caacactgaa
     2341 ggcaatgaat ttatttcttt taaaaaaata aactttgtaa taatctcttg tcttaactca
     2401 ttgagattat taaaatttaa tttgttttca aattgatcac ttagtgctct aaatattttc
     2461 cattcttctt ttgattctcc tatgggatta tagcttttag tagtttgcat gacgcgtcct
     2521 tccatattta caaaagtaga tgttttttct gggaaggccg ggataggcaa aattatatca
     2581 gcaatattag ctgttgcatc cccatgatgg cctaaataga caacaaatga atcttgaagt
     2641 attgaaagat ctatttcatc cagacctaat aaaaaaacaa ccggtttgta attttgaata
     2701 tgttgtttta atgagttgtt ataatcaaga tcaaaaaaat tattatagaa accgatatct
     2761 aaagccccta ctcgagaaat atcttgctga aggatattta agccattaaa attatggtta
     2821 gttatttgat atttttttgc gatttctgca cagactttta atatatcctt accatcagag
     2881 aaatttatag cagaagtgcc aataataatt aaaggttttt ttgccttctt caacactgta
     2941 caaaaatcag attgatcatt taaaatatca tttaaataat caatgttatt ggaaaggtgt
     3001 ttatatgtat aagttaaatc aattgctttt ccaattaggc caattttgca attgttgtga
     3061 atataagctt ttctaattcg tgcatttaaa acagttgctt cccaccttgg gttacttcca
     3121 atgattagta tagcatctgc atcatctatt ttctggattg aggaattaaa aatatagcta
     3181 tctctttggt ttgcaataaa ttgagcatta tcatatctgc agtcataaga agatgatcca
     3241 atagagttta aaaatgattt tgctgaataa agagtttcaa tatctgtaag cttaccactt
     3301 agtgctaaag tcttgttttt atttctatta ttaatttcag ttacaataat agataaggca
     3361 gtattccaat ctactatttg aaattttgaa ttttttctca tgaaaggttt gtctagtctt
     3421 tgttttgtaa ggccatcaat cgcaaagcgt gatttatcat ttatccattc ttcattaatt
     3481 tcttcattga gtcttggtaa aactcgcaat acagttttac caagggagtc aattctaata
     3541 ctcgaaccca ttgcgtcaaa tacatctatt gtttctgttt tttttagttc ccaaggtctc
     3601 gccttaaatt cgtaaggctt acttgttaaa gcgccaacag ggcataaatc tataacattc
     3661 cctgataatt ctgattttat tgttttctca agatatgttg taatttctgc attttcccct
     3721 cttcctaaca tacccaagtc atcaactcca gccacctcag tcgaaaatct cacacaccga
     3781 gtacaatgaa tgcatcttgt cataatagta cctaccaaag ggcccatatg tttattattt
     3841 accgctcttt tattctcttc atatcttgat ttatcgaaac cgtaatgaag agcttgatct
     3901 tgtaagtcac attctccacc ttgatcacag attggacaat ctaaaggatg atttataagt
     3961 aaaaattcca ttactcctct tcttgcactt tttactaaat cagtatttgt tttaataacc
     4021 atcccttctc cagctggcat tgcacaagaa gcaattggtt ttggagattt ctccatttca
     4081 acaagacaca ttctacagtt tcctgcgatt gacagtttat catgataaca aaatcttggt
     4141 atttccacat tggcaagttc acaagcctgc attactgtca tgccattttc aaattcaatt
     4201 tctctattat caatcgtaat ttttggcatt acgcaacagc ctttctttgt tgattttctt
     4261 caatacgctt ctcaatttct ggcctaaagt gcctcattaa accttgtatt ggccatgcag
     4321 cggcatcacc taaggcacat attgtatgtc cctctacttg tttggtgaca tctaataatt
     4381 tatcaatatc ttcatgatga acatttccat ctatcatttt tttcataatt ctaaacatcc
     4441 aaccggtacc ttctcgacat ggagtacact gaccacaact ctcatgttta taaaaatatg
     4501 acagtcttgt aatcgcctca acaatatcag tggttttatt cattacaata actgctgctg
     4561 tccctagacc gctttgaacg ccttgcagac tatcgaaatc cattttaatg ttatcacata
     4621 tagattttgg tatcagtgac acactagcac ctcctggtat aactgctagc agattatccc
     4681 aacctccaat tacgccacca gcatgttttt caattaattc ctttaggggg atacccattt
     4741 cttcttccac attacatgga ttattaacat gaccagaaat actgaaaatt ttagtaccag
     4801 tatttttggg atttcctata cctgcaaacc attgtgctcc gcgtcttaaa attgtgggta
     4861 ctacagcaac agtctcaaca ttagttacag tagtaggaca tccatacaaa ccagcgccag
     4921 ctggaaaagg tggttttagt ctcggttgcc ctttttttcc ttctaagctt tcaagaagag
     4981 ctgtttcttc accgcaaatg taagctcctg cccctctatg taaaaaaatg tcaaaatccc
     5041 acttagaacc acatgcattt tttccaatca agcttgcgac atatgcttca tcaattgctt
     5101 tttgtaaatt ggatgactcg ttataatatt ctccccttat ataaatataa caagcatgtg
     5161 cctgcatagc aaaaccggcc actaagcatc cttctagaag aagatgtgga tcatttctta
     5221 aaatttctct atctttacaa gttcctggtt ctgattcgtc tgcgtttatt acaagataat
     5281 gaggtt
//
LOCUS       DTVV01000030            2068 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1107314, whole genome shotgun sequence.
ACCESSION   DTVV01000030 DTVV01000000
VERSION     DTVV01000030.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1107314"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="EYO86_00415"
     CDS             complement(<1..925)
                     /locus_tag="EYO86_00415"
                     /inference="COORDINATES: protein motif:HMM:PF00370.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41534.1"
                     /translation="MQKETQFNVVLDIGKTNVKLYLLHKNNSVVKIFKTKQKVHFYKR
                     KIKLLNAPQLIDWLSKKLKIISKNHSLNKFVCTAHACSLAFIDEKDEEIIAVTDYEYQ
                     FDKFAKEYKKILPSFNKTYTPLLEGGLNLGQQIYFLSKQFPEVLKKTKFILSYPQYIS
                     WKLSNTFSSEISYIGCHSHFWNLSSSKYSSIINKLKIRKKLPTIRKAWEIIGKIKINN
                     KVLKILNGVHDSNASYLYFKNSNLKNFTLISSGTWYIIFNQKTKLKELKANLDMLCNI
                     DVFGNTVPTMRFMGGREFDELIKKLKITTINR"
     gene            complement(925..>2068)
                     /locus_tag="EYO86_00420"
     CDS             complement(925..>2068)
                     /locus_tag="EYO86_00420"
                     /inference="COORDINATES: protein motif:HMM:PF13561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC41535.1"
                     /translation="LKKNDHNLKNIVVENIKKYKSTYSKYYNRNKDKNSPNLRDPYPV
                     IVLIPEYGMLSFAKNKTTARIASEFFGNAINVMKGAEGVTNYTGLTEKEAFGIEYWEL
                     EEAKLKRMPPEKALAGKVAIITGAAGGIGTATAKKFLAEGCCVILADIDKQALSMVEK
                     ELSSEFNSEVISSIVMDVTNETQVKKAVRQGIETFGGIDILVANAGFSSAAAFEKTPT
                     KLWDKNMNVLSKGCFIISREVYSQMIDQDLGGSMIFIASKNALSASKEASAYSVAKSS
                     ILHLSRSIALEGASYKIRSNVVNPDAVIRNSKIWDSEWKTQRAANYKIPANKLEEFYK
                     NRSLLKVSILPEDIAEGIFFFASSVSKKSTGNILNVDGGNITAFTR"
BASE COUNT          613 a          342 c          284 g          829 t
ORIGIN      
        1 gtctgtttat tgtagtaatt tttaattttt ttattaattc atcaaactca cgtccaccca
       61 taaatctcat tgtgggaaca gtgttaccaa aaacatcaat attgcataac atgtccaagt
      121 ttgcttttag ttcttttaat tttgtttttt gattgaaaat tatataccaa gtccctgaag
      181 atataagggt gaagtttttt aaatttgaat ttttaaaata caagtatgaa gcattactat
      241 catgaacacc attcagtatt tttaggactt tgttattaat ttttattttt cctataatct
      301 cccatgcctt tcttattgtt ggtaattttt ttcgaatttt aagtttattt atgattgaag
      361 aatatttact tgaagagaga ttccaaaaat gactatggca gccaatatat gatatttcac
      421 tactaaatgt attggataat ttccaactaa tatattgagg ataggacaaa ataaattttg
      481 tttttttgag cacctctgga aattgcttag ataaaaagta aatttgttgt cccaaattaa
      541 gcccgccttc caaaagaggg gtatatgttt tattgaatga tggtagaatt tttttgtatt
      601 cctttgcaaa tttatcaaat tgatattcat aatctgtgac agcaataatt tcttcgtctt
      661 tttcatcaat gaaagccaat gaacatgcat gtgctgtaca aacaaattta tttaaagagt
      721 gattcttgga aattattttt aatttctttg aaagccagtc tattaattga ggagcattaa
      781 gtaattttat ttttctttta taaaaatgta ctttctgttt ggttttaaat atttttacaa
      841 ccgaattatt cttatgaagc aaatataact tcacatttgt tttaccaata tcaagaacaa
      901 cattaaattg agtttctttt tgcattatct tgtaaatgct gtaatatttc ctccatccac
      961 attcaagata tttcccgttg attttttaga tactgatgat gcaaagaaga atatgccttc
     1021 agctatatct tctggtaaaa tactaacttt cagtaaactt ctatttttat aaaattcctc
     1081 taatttatta gccggtattt tataatttgc tgctctttgc gttttccatt cactgtccca
     1141 aatttttgag ttccttatta ctgcatcggg attaacaaca ttggatctga ttttataact
     1201 tgctccttct aaggcaattg atcgtgataa atgtaaaatt gagctttttg caacactata
     1261 ggcagatgcc tcctttgaag cacttaacgc gttcttactt gcaatgaata tcattgaccc
     1321 tcccaagtct tgatcaatca tttgggaata gacttctctg ctaatgataa aacatccttt
     1381 tgataatacg ttcatgtttt tgtcccacaa tttagtaggt gttttttcga atgctgccgc
     1441 tgaagaaaaa cctgcgtttg caactagaat gtcaattcct ccaaaggttt ctattccttg
     1501 tcgaacagct ttttttacct gagtttcatt ggtaacatcc attacaattg aagatattac
     1561 ttctgaatta aattctgatg acaactcttt ttcaaccata gatagtgctt gtttatctat
     1621 gtctgctaaa atgacacaac agccctctgc tagaaatttt tttgcagttg ccgtaccaat
     1681 tcccccagct gctccagtta ttattgctac ttttcctgct aatgcttttt caggaggcat
     1741 gcgtttaagt ttagcttctt ccaactccca atattcaatt ccaaatgctt ctttttctgt
     1801 taagcctgta tagtttgtaa caccttcagc gcctttcatt acattaatag cattaccaaa
     1861 aaattcactg gctattcgcg ctgttgtttt attttttgca aaagaaagca taccatattc
     1921 tggtatcaaa actattacgg gataagggtc tcttaaatta ggtgagtttt tatccttgtt
     1981 tctattataa tattttgagt aagtactttt atatttttta atattttcaa caacaatatt
     2041 ttttaaattg tgatcattct tttttaaa
//
LOCUS       DTVV01000031            3315 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1111103, whole genome shotgun sequence.
ACCESSION   DTVV01000031 DTVV01000000
VERSION     DTVV01000031.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1111103"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="EYO86_00425"
     CDS             complement(<1..720)
                     /locus_tag="EYO86_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIC41536.1"
                     /translation="MPIKIGLIGKTNTGKTTFFNSSTLSTDEISTYPFTTKKPSTSIG
                     HAITLCVHKEFNVQDNPNNSKCSDGWRYIPIELIDLPGLIKDAWKGKGLGNQFLSIAA
                     QSDALLHVVDASASIDSSGRITETGTGDPVSDFADIEEELNMWYQKILEGNRDKLLKM
                     IKSENDQISALTELYQGLGIKKNHVMETLRITGLEGKDIENYNLHESKKFSTQLRKVS
                     KPTLIVANKIDVPGADKNFVRL"
     gene            complement(741..1124)
                     /locus_tag="EYO86_00430"
     CDS             complement(741..1124)
                     /locus_tag="EYO86_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41537.1"
                     /translation="MSNPTFSEFYKSLLQLVKTFEEKNMMLKVEEDAALNIIRIFGEN
                     ADSVSRAKSSLEDVVELAYATAEHHPYWALLYNSSLISKAVLEKWSDDLSEEDLGEIQ
                     WMVSELQNSCDKLMNKITEQGTRDK"
     gene            complement(1127..1546)
                     /locus_tag="EYO86_00435"
     CDS             complement(1127..1546)
                     /locus_tag="EYO86_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIC41538.1"
                     /translation="MVFMERDSYTDKEIKDILTLKKIAVIGMSRHSNKAAHFVPKYLS
                     EQGYDITPINPIAEEILGKKSYPDILSVADPIDIVDIFRPSEQVLPVVQDAIKIKPKV
                     IWLQEGIHNLEAEELARKHGIKVVYNRCMLAEHQRLF"
     gene            1588..2586
                     /locus_tag="EYO86_00440"
     CDS             1588..2586
                     /locus_tag="EYO86_00440"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIC41539.1"
                     /translation="MYTSFDNVGLLKVLTLLKSHKSEFLSGQDLSDVLKISRVAVWKH
                     IKKIRSEGYTVESKQNLGYKLIGNTERLLPWEITENLNTKFIGKRIYYFDSMDSTQNF
                     AVEIAPNRKENGTVVISKKQTEGMGRLKRKWKSPVGGIWLSVIVHPKFSTSQITLVPI
                     ATSLALHKAVENVLGIKPMLKWPNDLTLKKKKVAGILLDTSIQSNKIEFLVLGVGINF
                     KIKPRNLEKLLKKTPNFYGVSTLVEKNEKPLPLVKQFLYELENVFQMINSGSNKKIVT
                     EWTKRSSTIGKNVSVITDDGRFNGKAIKLDIDGGLIITRGKKTKKLFVGDVLHKNQ"
     gene            2632..>3315
                     /locus_tag="EYO86_00445"
     CDS             2632..>3315
                     /locus_tag="EYO86_00445"
                     /inference="COORDINATES: protein motif:HMM:PF07719.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC41540.1"
                     /translation="MFKNFIKISVVFSFAIVFLTGTSFAEPINTVEQVKDAEEYFLNG
                     EYRMAIEIYDDILEVFPTDTKIREMKAISLSNMRLYTTLAAQPFSGVSTQYDKYYLNE
                     KSMLEFYTVLETDPNSVIALNGLGLGFGNFGEYGEAKKYFDRALKLDPDNHVSKNYLE
                     YLEKIIKKYPYKPTEKPAYLLKFEENTIPHWIRSNASWWAADKISDNDFISGIQYLIE
                     NKIIKLSSQN"
BASE COUNT         1107 a          549 c          570 g         1089 t
ORIGIN      
        1 aaggcgtaca aaatttttat cagcacctgg aacatcaatc ttatttgcta caatcagcgt
       61 tggttttgat acttttctaa gttgtgttga aaactttttg ctctcatgta aattgtaatt
      121 ttcaatgtcc ttgccctcaa gcccagtaat ccttaatgtc tccataacat gatttttttt
      181 tatccctaaa ccctgataca gttcagttaa tgcagatatc tgatcatttt cagatttaat
      241 catttttagc agtttatcgc gattgccctc caagattttt tgataccaca tattcagctc
      301 ttcctctata tcggcaaaat cagatactgg atcaccagtg ccggtttctg tgattcgtcc
      361 agaagaatca atgctcgcag atgcgtctac cacatgtagt aatgcgtcag attgtgctgc
      421 tatagaaaga aattggttgc ctaacccttt tcccttccat gcatccttaa ttaatcccgg
      481 caaatcaatt aattcgatag gaatatacct ccaaccgtca ctacatttgg aattatttgg
      541 gttatcttgt acattgaatt ccttatgaac acaaagcgta atggcatgtc caattgatgt
      601 agacggtttt tttgttgtaa aaggatatgt tgaaatttca tcggtggata acgtagatga
      661 attaaagaaa gttgtttttc cagtgtttgt tttgccaata aggccgattt taatgggcat
      721 ggttgaaaaa catattcata ttatttatct ctggtacctt gttcagttat tttattcatt
      781 aatttatcgc acgagttttg caattcagaa accatccact gaatttcgcc aagatcttct
      841 tcagacagat catcgctcca tttttccaat actgctttgg aaataagtga agaattgtaa
      901 agtaatgccc aatacggatg atgttcagca gttgcataag ccagttccac aacatcttcc
      961 aagctgcttt tagcacgaga aactgaatca gcgttttctc caaatatccg aattatgttc
     1021 aaagcagcat cctcctctac cttaagcatc atatttttct cttcaaaagt tttaaccagc
     1081 tgaagtagtg atttgtaaaa ttcagaaaac gttggatttg acatgatcag aaaagacgtt
     1141 ggtgctcggc caacatacat cgattgtaaa ctaccttaat tccatgtttt cttgccagtt
     1201 cctctgcctc tagattatgg atgccttcct gaagccaaat cactttcggt ttaattttaa
     1261 ttgcatcctg aacaaccggt aaaacttgtt ctgatggtct gaatatatcc acaatatcta
     1321 tcggatctgc cacagataaa atatcaggat atgatttttt ccccaatatt tcttcagcta
     1381 tagggttaat cggtgtgata tcatatccct gctctgacaa atattttggg acaaaatgtg
     1441 ctgccttatt agaatgtctt gacataccaa taactgcgat tttttttaat gtaagaatat
     1501 ctttgatttc tttatctgtg taagaatctc tttccatgaa cactatgaaa ctaatcactt
     1561 tttataattt ttaaggtttg taatagtgtg tatacttcgt ttgataatgt tggtctgctc
     1621 aaagttttga cattactaaa gtcgcacaaa tctgaatttt tatctgggca agatttgagc
     1681 gatgttctca aaataagccg tgttgctgtc tggaaacata tcaaaaaaat tcgttcagag
     1741 ggatacactg tagaatcaaa acaaaatcta gggtacaaat tgattggtaa tacggaacgt
     1801 cttctgccat gggaaataac tgaaaattta aatacaaaat tcataggaaa aagaatttat
     1861 tattttgata gcatggattc aacacaaaat tttgcggtag aaattgcacc taatagaaag
     1921 gaaaatggga cggttgtcat atcaaaaaaa caaaccgagg gaatgggcag attgaaaaga
     1981 aaatggaagt caccggttgg tggaatttgg ttatcagtga ttgttcatcc aaaatttagt
     2041 acttcgcaga tcacacttgt accaattgca acttcacttg cattacacaa agctgttgaa
     2101 aatgtattag gaattaagcc tatgctaaaa tggccaaatg atctgacatt aaaaaagaaa
     2161 aaggttgctg gaatattact tgacacatct atacagtcaa ataagattga atttctggtt
     2221 ttaggtgtag gaattaattt taaaataaaa ccgcgtaatt tagaaaaatt gctgaaaaaa
     2281 acaccaaatt tttatggtgt tagtacacta gttgaaaaaa atgaaaaacc attaccgttg
     2341 gtcaaacaat ttctatatga gttggaaaat gttttccaga tgatcaattc tggatctaat
     2401 aaaaaaattg tgaccgagtg gacaaaaaga tcatctacaa taggcaaaaa tgtttcagtt
     2461 atcactgatg atggtcgttt caatggaaaa gcaataaagc tagatattga tggtggatta
     2521 atcattacca ggggtaagaa aaccaaaaaa ttgtttgtgg gagatgtttt acacaagaat
     2581 caatagttat attttaatta gaattttttt aaattaaaat ctgaaaccag aatgtttaaa
     2641 aatttcataa agatatcagt tgttttctca tttgccatag tttttctcac tggaacatct
     2701 tttgctgagc ccatcaatac agtagagcag gttaaggatg ctgaagaata tttcctcaat
     2761 ggtgagtata gaatggctat agaaatctat gatgatatac ttgaggtttt tcctacagat
     2821 actaaaatac gagaaatgaa ggctatttcg ctgagtaata tgaggctcta tactacctta
     2881 gcggctcagc cattcagtgg tgtctcaaca caatatgaca aatattattt aaatgaaaaa
     2941 tctatgctgg aattctatac tgtattagaa actgatccaa acagtgtcat agcattaaat
     3001 ggtttgggat taggctttgg gaattttgga gagtatggtg aagcaaaaaa atattttgat
     3061 agagctctaa aattggatcc tgataatcat gtatcaaaaa attatcttga atatttagaa
     3121 aaaattataa aaaaatatcc atacaagcct acagaaaaac ctgcatatct tttaaaattt
     3181 gaagaaaata ccataccaca ttggattaga agtaacgcaa gctggtgggc tgctgataaa
     3241 ataagcgata atgactttat ctctggcatt caatacctta ttgaaaataa aatcattaag
     3301 ctaagttcac aaaat
//
LOCUS       DTVV01000032            1216 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1112212, whole genome shotgun sequence.
ACCESSION   DTVV01000032 DTVV01000000
VERSION     DTVV01000032.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1112212"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            43..>1216
                     /locus_tag="EYO86_00450"
     CDS             43..>1216
                     /locus_tag="EYO86_00450"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011452720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIC41541.1"
                     /translation="MKDVKIKPLTINFGPQHPAAHGVLRLVLEMDGEVIERVDPHIGF
                     LHRGTEKLIESKTYLQSVPYFDRLDYVSPMCQEHGFALAVEKLLKIHAPIRAQYIRVM
                     FSEITRILNHLLALPAMAMDVGAMTPFLWSFEQRELLMEFYERVSGARLHAAYFRPGG
                     VYRDLPKNLLSDISKWAKKFPKFINDLELILTENRIFRQRTVDIGKVNATDAINFGFT
                     GPMLRASGVKWDLRKIDSYEVYNKLKFDIPVGKQGDCFDRYQVRVEEMKQSLNIILQC
                     IDRIPKGIVMVKNNKITPPKRKSMKQSMESLIHHFKIFTEGYKVPKGETYTAVEAPKG
                     EFGVYLISDGTNKPYKCKIRAPGFAHLQAMDFCSKGHMLADIVAILGSMDIVFGEVD"
BASE COUNT          455 a          164 c          230 g          367 t
ORIGIN      
        1 cctggtgacg aaaaagctaa taattaattt tttataaaaa taatgaaaga tgtaaaaata
       61 aagccattaa ctataaattt tggaccgcaa catccggcag cacatggagt ccttagattg
      121 gttttagaaa tggatggtga agttattgaa agagttgatc ctcatattgg atttttacat
      181 agaggtacgg agaaactaat agaaagtaaa acttatttac aatctgtacc ttattttgat
      241 cgactagact atgtttctcc aatgtgccaa gaacatggtt ttgcattggc agttgaaaag
      301 ttacttaaaa ttcatgctcc aataagagct caatatatta gagtcatgtt ttctgaaatt
      361 acaagaatat tgaatcattt attagcatta ccagcaatgg caatggacgt tggcgccatg
      421 accccttttt tatggtcatt tgaacaaaga gagttattga tggaatttta tgaaagagta
      481 agtggcgcta gacttcatgc agcatatttc cgacctggag gtgtatatag agatttacca
      541 aaaaaccttt tatcagacat aagcaaatgg gcaaaaaagt ttcctaaatt cataaatgat
      601 ttagagttga tattaacaga aaatagaatt tttagacaaa gaacagttga tataggaaaa
      661 gtaaatgcta ccgatgcaat taattttggt ttcacaggac caatgttaag agcttcaggt
      721 gtcaaatggg atttaagaaa aattgatagt tatgaagtat ataataaatt aaaattcgat
      781 attcctgtag gtaagcaagg cgattgtttt gataggtatc aagtaagagt agaagaaatg
      841 aaacaaagtt taaatattat actgcaatgt atagatagaa ttccaaaagg aatcgtaatg
      901 gtaaaaaata ataaaattac gccccctaaa agaaaatcaa tgaaacaatc tatggaatca
      961 ctaattcatc attttaaaat ttttactgaa ggatacaaag tacctaaagg cgaaacctat
     1021 acggcggttg aagctccaaa aggtgaattt ggggtttacc ttatttcaga tggcactaac
     1081 aaaccttaca aatgcaaaat tagagcacct ggttttgccc acttacaagc aatggatttt
     1141 tgtagcaaag gtcatatgtt agcagacata gtcgcaatat tgggatcaat ggatattgta
     1201 tttggagaag ttgata
//
LOCUS       DTVV01000033            1383 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1116835, whole genome shotgun sequence.
ACCESSION   DTVV01000033 DTVV01000000
VERSION     DTVV01000033.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1116835"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="EYO86_00455"
     CDS             complement(<1..702)
                     /locus_tag="EYO86_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIC41542.1"
                     /translation="MNSSAVRRTFLNYFKKNNHEIVHSGPLVPENDPTLMFANSGMVQ
                     FKNIFTGKEIREYSRAVTAQKCVRAGGKHNDLENVGYTTRHHTFFEMLGNFSFGDYFK
                     DLAIELAWNLITKEYGIDKNKLVVTVFEEDMEAYDLWKKISGLKDDKIIKINTSDNFW
                     SMGDTGPCGPCSEIFYDHGDKFFGGPPGSKDEEGDRFIEIWNLVFMQFEQISKEERIK
                     LPKPSIDTGMGLERIT"
     gene            complement(797..>1383)
                     /locus_tag="EYO86_00460"
     CDS             complement(797..>1383)
                     /locus_tag="EYO86_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HIC41543.1"
                     /translation="GDSLPGLQARLMSQALRKLTSSIAQSNTLVVFINQLRMKIGVMF
                     GSPETTTGGNALKFYASVRLDIRRIGAIKDKEEIIGNQTRVKVVKNKVAPPFKVVEFD
                     IMYGEGISKLGELVDLGVKAEIIDKSGSWFAYNDHKIGQGRDNVKTYLKENPNIAKEI
                     ETRILENAGIVEKAMMEGEISPEKKETDIIKEKE"
BASE COUNT          407 a          282 c          178 g          516 t
ORIGIN      
        1 ggtaattctt tctaagccca ttccagtgtc aatcgaaggt ttgggaagtt taattctttc
       61 ttcttttgaa atttgctcaa attgcataaa tacaagattc cagatttcta taaatctgtc
      121 cccttcttca tcctttgacc ccggaggacc tccaaaaaac ttatctccat gatcataaaa
      181 aatttctgag caggggccgc atggtcctgt atcccccatt gaccagaagt tgtctgatgt
      241 attgatttta attattttat catcttttag tcctgaaatt tttttccata aatcataggc
      301 ttccatatct tcttcaaaaa cagttacgac caatttattt ttatctattc cgtattcttt
      361 cgttatcaaa ttccaagcta gttcaattgc taaatcctta aaataatcac caaatgaaaa
      421 atttcctaac atttcaaaga aagtgtggtg tctcgttgta taacctacat tttcaagatc
      481 attgtgtttt ccaccagctc tgacacactt ttgtgctgtc acagcacggg aatattctct
      541 aatttctttg ccagtaaaaa tatttttaaa ctgaaccatg ccagaattag caaacattaa
      601 agttggatca ttttctggca ctaatgggcc tgaatgaact atctcatgat tatttttctt
      661 aaaataatta agaaacgttc ttctaactgc ggatgaattc ataacgattc tattataaca
      721 acttttaata ttgtctattt taaattaaaa aaggcgcttc taacaagcgc cttggcaagg
      781 atagaataac tttttattat tctttttctt taataatatc cgtctctttt ttttcaggac
      841 taatttcacc ttccatcatt gctttttcaa cgattccagc attttcaaga attctagttt
      901 ctatctcttt agcaatattg ggattttctt taaggtaagt ttttacatta tcccttcctt
      961 gtcctatttt atgatcatta tatgcaaacc aagatccgga cttatctata atttctgctt
     1021 taactcccaa atcgacaagt tcccctagct ttgaaattcc ttctccatac ataatatcaa
     1081 actcaaccac tttaaaagga ggggcaactt tattttttac aacttttact ctagtttgat
     1141 ttcctattat ttcctcttta tcttttattg ctccaattct tctaatatct aaacgcacag
     1201 atgcataaaa ctttaaagca ttgcctccag ttgtagtttc tggactgcca aacataaccc
     1261 ctattttcat tcttaactga ttaataaata ctacgagagt attggattga gctatagagc
     1321 tagttaactt tcgaagagct tggctcatta gtcgagcttg caaaccaggt aaagaatcgc
     1381 cca
//
LOCUS       DTVV01000034            1179 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1119090, whole genome shotgun sequence.
ACCESSION   DTVV01000034 DTVV01000000
VERSION     DTVV01000034.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1119090"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            177..1091
                     /locus_tag="EYO86_00465"
     CDS             177..1091
                     /locus_tag="EYO86_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIC41544.1"
                     /translation="MRVTENLRLKLLSPPTDQVRVVMDTDTFNEIDDQFAIVQMMLSP
                     DRLKVEAIYAAPFYNDRAKDPGHGMEQSYDEILRLLERLNISSKGLVHKGVTEYVGLE
                     KNAREADAVNDLISRARSGSPENPLYVIAIGAISNVASAIIKAPDIIDRIVVVWLGGH
                     ALHWPHTQEFNLKQDVGGAQALLDSGVPLILVPCMGVVSHLHSSVPEIEKYIEPHGEI
                     GAFLAMRFKEYRSDHKGWSKEIWDMAAVAGVLDSTWAQSVIISSPVLNDNITWSVDHR
                     RHAIRYVTAINRDAILKDFIKKLEVFNT"
BASE COUNT          392 a          185 c          232 g          370 t
ORIGIN      
        1 atagatctaa tttttttaga aaaaattttc tatttataaa aacttcaaaa aatattttta
       61 tacttttatc tatgattagt tgatgttaaa tttaattttg taaatatctt aaatgataat
      121 tttatttata tagaaagaaa aaaatgggct attttgaaaa aaagattgaa aataaaataa
      181 gagttaccga aaacctccgt ctaaaacttt tatctccacc gacagatcaa gttcgtgttg
      241 tgatggacac agatacattt aatgaaattg atgatcaatt tgctatagtt caaatgatgc
      301 tatcaccaga tcgcttaaag gttgaagcaa tatatgctgc tccattttat aatgatcgcg
      361 caaaagatcc aggacacgga atggagcaaa gttatgatga aattctacgc ttacttgaac
      421 gactgaatat ttcatcaaaa ggattagttc ataaaggggt aactgaatat gtaggtcttg
      481 aaaaaaacgc acgggaagct gatgctgtaa atgatctaat atctcgggca agaagtggat
      541 ctccagaaaa tccactttac gtaatagcaa ttggagctat tagcaatgta gcctcagcta
      601 ttattaaggc tcccgatata atagaccgga tagtggttgt ctggctcggc ggtcatgctc
      661 ttcattggcc acatacacaa gaatttaatc tcaagcaaga tgtggggggt gcccaagctt
      721 tgttggatag cggtgttcct ctgattcttg ttccatgcat gggagttgta tctcatctcc
      781 acagttctgt accggaaata gaaaaatata tagagccaca tggtgaaata ggtgcttttc
      841 ttgcgatgcg ctttaaagag tatagatctg accataaagg ttggtccaaa gaaatttggg
      901 acatggcggc ggttgctggg gtgctagatt ctacttgggc tcaaagtgtg ataatttctt
      961 ctccagtttt aaacgacaat ataacttgga gtgttgatca tcgtcgacac gcaattcgct
     1021 atgtcacagc aattaatagg gatgccattt taaaagattt tattaaaaaa ttagaagtgt
     1081 tcaatactta acaaaactta gcttccccga gttttattta gtagcgaata tttaatttaa
     1141 taccaccaac cacgagggat gttgagggga attaattaa
//
LOCUS       DTVV01000035            1039 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1119292, whole genome shotgun sequence.
ACCESSION   DTVV01000035 DTVV01000000
VERSION     DTVV01000035.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1119292"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1039)
                     /gene="uvrC"
                     /locus_tag="EYO86_00470"
     CDS             complement(<1..>1039)
                     /gene="uvrC"
                     /locus_tag="EYO86_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HIC41545.1"
                     /translation="EKSAGLQGQEIIKFIAKTLPHKPGIYQMENEKGEILYIGKAKNL
                     AKRVINYTSLNNLTRRLQRMVSLTSRMNFVVTNTEIEALLLECNLIKRHKPRFNIILR
                     DDKSFPYILINKEHTFPRIQKYRGAKKFKGDYYGPFVSPSVADYTLISLQKTFLLRSC
                     SEGTFNNRSRPCLLYDIKRCSAPCVNYISEKDYLESIEDANKFLGGDTKKIEKKLNFQ
                     MVLASKNQMFEEAAQLRDRIKSINQIQKYQSVYIKDMRNIDIFAIKVIGGKSCIHGKF
                     YRNGSNYGNKSFFPMHQEFTEEKEILESFLYQFYSNRDAPPKILINLNHTFFREVEYT
                     LNLKNKFKTKI"
BASE COUNT          322 a          149 c          152 g          416 t
ORIGIN      
        1 aaaattttcg ttttgaattt gttttttaaa tttaaagtat attcaacttc tcgaaagaat
       61 gtatgattta ggtttattaa gattttagga ggagcatccc tatttgaata aaattgataa
      121 agaaatgatt caagtatttc tttttcttcg gtaaattcct gatgcattgg aaagaaagat
      181 ttatttccat agttactacc gttacgataa aattttccat gtatacaact tttgccacct
      241 attactttaa ttgcaaaaat atcaatatta cgcatatctt taatgtaaac agattgatat
      301 ttttggattt gattaattga ttttattcta tcgcgtagtt gtgctgcttc ttcaaacatt
      361 tgatttttac tggctaaaac catttgaaaa ttaagttttt tttcaatttt ttttgtatct
      421 cctccaagga atttattagc atcttcaata ctttcaagat aatctttttc tgaaatataa
      481 ttcacacacg gagcactaca tcttttgata tcatagagta agcaaggtcg tgatcgatta
      541 ttaaatgttc cctcagaaca actacgaagc aaaaaggttt tttgtaaaga aatgagagta
      601 taatctgcaa cagatggaga tacaaatgga ccatagtaat cacctttgaa ttttttagct
      661 cctctatatt tttgtattct tgggaaagta tgttctttat taataaggat ataagggaaa
      721 gatttatcat cccgaagaat aatattaaag cgcggcttgt gtcttttaat tagattacat
      781 tctaataata aggcttctat ctccgtatta gttacaacaa agttcattct tgatgttagg
      841 cttaccattc gttgtaaacg acgtgtaaga ttgtttaatg aagtgtaatt aataactctt
      901 ttcgcaaggt tttttgcctt cccaatatat aaaatttctc ctttttcatt ctccatttga
      961 tatatgcctg gtttatgtgg aagtgtttta gctatgaatt ttattatttc ctgtccttgt
     1021 aaaccagctg atttttctt
//
LOCUS       DTVV01000036            2078 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1122208, whole genome shotgun sequence.
ACCESSION   DTVV01000036 DTVV01000000
VERSION     DTVV01000036.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1122208"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /gene="pdxH"
                     /locus_tag="EYO86_00475"
     CDS             <1..428
                     /gene="pdxH"
                     /locus_tag="EYO86_00475"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIC41546.1"
                     /translation="GFVFYTNLQSKKGISLLSNSNASLTFHWKSLLKQIRIEGRAHQV
                     TSKEADDYFNSRPMESRIGAWASNQSSELSDRQELIDKISLYKKKFQQNSISRPPHWT
                     GFRVEPNLIEFWKDMPFRLHDRIEYKKTDNKWISRRLYP"
     gene            complement(430..867)
                     /locus_tag="EYO86_00480"
     CDS             complement(430..867)
                     /locus_tag="EYO86_00480"
                     /inference="COORDINATES: protein motif:HMM:PF01467.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HIC41547.1"
                     /translation="MNDIILVSGGFDPIHSGHIDLIKDASKYGDVVVLLNSDAWLVQK
                     KGKEFLPYEERKIIMSALKNVIDVISFDDSDQTCVEGIRKAIEKYPNQKIKFANGGDR
                     SHNTTPSPEKAYCEQKKVEVLWGIGGHNKSNSSSWILKKWFQK"
     gene            complement(869..1510)
                     /locus_tag="EYO86_00485"
     CDS             complement(869..1510)
                     /locus_tag="EYO86_00485"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:PRK00455.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIC41548.1"
                     /translation="MSDTDYQNTVAEILINIGSVRFSFENPFILTSGLKSPVYVDCRK
                     IISFVNERNTILNLAIEYFKNNNINFDILAGGETAGIPYAAFFSELLQKPMIYVRKKT
                     KGFGKDQQIEGSFKKNQKTILIEDLATDGGSKVIFIEALRKAELLVSDIFVVFYYDIF
                     DINNSPLSSLDVKIHSLCTWKDIINFLKKKKIYSEEQIHNLEKFLQNPGKWRD"
     gene            complement(1510..1983)
                     /locus_tag="EYO86_00490"
     CDS             complement(1510..1983)
                     /locus_tag="EYO86_00490"
                     /inference="COORDINATES: protein motif:HMM:PF00582.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIC41549.1"
                     /translation="MNDDRYILVIADGSPEMSVALEYACARSKKTGRKIIIATFIEPL
                     DVLTTQGVTEIMKNEAREESERILHLAASVVKESTGIIPILHTREGELILELKKLIEE
                     EEKIHVLVLAANANEKEKDPGPIIHSLVTKEMSNLRIPVMIVPGNLSKEHISQIT"
     gene            complement(1986..>2078)
                     /locus_tag="EYO86_00495"
     CDS             complement(1986..>2078)
                     /locus_tag="EYO86_00495"
                     /inference="COORDINATES: protein motif:HMM:PF09608.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41550.1"
                     /translation="AHEQSATYGLLSILFAVLSGLIAATIFRRI"
BASE COUNT          672 a          356 c          292 g          758 t
ORIGIN      
        1 atgggtttgt tttttataca aaccttcaaa gcaaaaaagg aatttcatta ttatcaaatt
       61 ctaatgcctc tctgacattt cattggaaaa gtttacttaa acaaattaga attgaaggta
      121 gggctcatca agtaactagt aaagaagctg atgattattt caacagtaga ccgatggaga
      181 gtagaattgg tgcgtgggca tctaatcaat caagtgaact ttcagatcga caagaattga
      241 ttgataaaat ttcactatat aaaaaaaaat ttcaacaaaa ttctatcagc cgtcctcctc
      301 attggacagg ttttcgagtt gaacctaatc tgattgagtt ttggaaggat atgccttttc
      361 gtctacatga tcgaatagaa tacaaaaaaa ctgataataa atggatttca agaagactct
      421 atccataaat tatttttgga accacttttt taaaatccat gagctagaat tcgatttatt
      481 atgtcctcca attccccaca gtacttctac ttttttttgt tcacaatatg ctttttcagg
      541 tgaaggcgtt gtattgtgac tacgatctcc accattggca aattttattt tttgatttgg
      601 atacttttca atagcttttc taattccttc aacacatgtt tggtctgagt catcaaaaga
      661 aattacatca attacatttt ttagggcact cataattatt ttacgctcct catagggcaa
      721 gaattctttc cccttttttt gtacaagcca agcatcagag tttaataaaa cgactacatc
      781 gccatatttt gaagcatctt ttattaggtc tatatgacca ctatgtattg gatcaaaacc
      841 tccgcttact aaaataatat cattcatatt aatccctcca tttgccagga ttttgaagaa
      901 atttttccaa gttatgaatt tgttcttctg aatatatttt ctttttcttt agaaaattta
      961 ttatgtcttt ccatgtgcat aaagagtgaa tttttacatc aagagatgat aatggcgaat
     1021 tgttaatatc aaaaatgtca tagtaaaaaa caacaaatat atcagaaact aatagttcag
     1081 cttttcttag tgcttcaata aaaataactt tacttccccc atcggttgct aaatcctcaa
     1141 ttaaaatagt tttttgattt tttttaaagc taccttctat ttgctgatct ttaccaaatc
     1201 ccttagtttt ttttctaaca taaatcattg gtttttgaag taactctgaa aaaaaagccg
     1261 catagggaat accagcagtt tcacctcccg ctaaaatatc aaaattaatg ttattatttt
     1321 taaaatattc tattgcgaga ttcagaatgg tatttctttc atttacaaaa gaaataattt
     1381 ttctacaatc aacatatact gggcttttta aacctgaggt aaggataaag ggattttcaa
     1441 atgagaatct aacagatcca atattaatca aaatttctgc aacagtattt tgataatctg
     1501 tatccgacat taagtaattt gagatatatg ttctttagat aagttgccag gaacaatcat
     1561 tactggtatt cgaaggtttg acatttcttt tgttactaat gaatgtataa ttgggcctgg
     1621 atctttttct ttctcattcg cattggcagc taaaaccaaa acatgaattt tttcttcttc
     1681 ttctattaat ttttttaatt cgagtattaa ttcaccttct cttgtatgca aaataggaat
     1741 tattccagta gattctttaa caactgaagc tgctaaatga agaatcctct cagattcttc
     1801 tcttgcttca tttttcatta tttcagtaac tccttgtgtt gttaatacat caaggggctc
     1861 aataaaagtt gctattatga tttttcttcc tgtttttttt gatctcgcgc aagcgtattc
     1921 caaggctaca ctcatttcag gcgacccatc agcaatgact aaaatataac gatcatcatt
     1981 cataattaaa ttctcctaaa tatcgttgca gctatcagcc cagataatac agcaaaaagt
     2041 attgataata aaccatatgt agcagattgc tcgtgggc
//
LOCUS       DTVV01000037            3816 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1128927, whole genome shotgun sequence.
ACCESSION   DTVV01000037 DTVV01000000
VERSION     DTVV01000037.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1128927"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="EYO86_00500"
     CDS             <1..804
                     /locus_tag="EYO86_00500"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIC41551.1"
                     /translation="GAYSELAGKNIYPDSKSIPCKTFEEMFESVRDKKANIAIVPIEN
                     SRSGRVGDTQRLIPESQLKIVGEYFLEVNHCLMGVPGSTLKDIKRIYSHEQGIAQCRK
                     KIINNKKDMVIVADTAGAAKMISETNSKEDAAIASNLAAKIYNLDILESSFQDFENNV
                     TRFLIMSNELNIPKVNEIDLMTTLVFEVRSIPAALYKSLGGLASNGVNMTKLESYINP
                     QGFDVAQFYMDIEGHPNNRNVKLALEEMKFFCKHIDILGVYKMSDFRKK"
     gene            859..955
                     /gene="ffs"
                     /locus_tag="EYO86_00505"
     ncRNA           859..955
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="EYO86_00505"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            964..2688
                     /locus_tag="EYO86_00510"
     CDS             964..2688
                     /locus_tag="EYO86_00510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:PRK09111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIC41552.1"
                     /translation="MIDKIEQEEFRVLARKYRPQVFDDLIGQETLVRTLTNAITNNRI
                     AHAYLLTGVRGVGKTTTARIIAMSLNCEDRIQEKCEPCAKCDSCVSIREDRNLDVIEM
                     DAASKTGVDDIREIIDNVKYKPVNSKYKIFIIDEVHMLSKNAFNALLKTLEEPPAHVK
                     FIFATTEVKKIPITIISRCQRFDLHRIATTVLTSHLMNLAKKENINIDKDAMALIVRS
                     ADGSVRDGLSLLDQAITNEKDKITANSIIDMLGLADRGNIFDLMDAIFQGDPVNALAI
                     FNRIHQAGADVVMIFDEMLNVTHFITQIKIAPELKEDIHIPELERNKGFEMAEKISMA
                     SLGMIWQVLFKGYEELQSGFHLFQQGEMIIIRLIYLYDGPSPGDLIKKIEEDKKLSLN
                     MSTSSESSSSVEKDPIINQVRNKNNEIKEKREANKEDLPNPIGVLTIKSYRHFVDLFY
                     QKKEGMLHTYLYNNAKLVSFKEGEVVVNAKSIADPHFPRTIAKLVSTWTGRIWQVSTS
                     ASNVGKTLNEEDLIQQQKEIEIMKNEPEVKAILGKFPGVTIHSITDIHQTADEKVFAT
                     KNKQTKEK"
     gene            2688..3011
                     /locus_tag="EYO86_00515"
     CDS             2688..3011
                     /locus_tag="EYO86_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIC41553.1"
                     /translation="MVNIGNMMKQAQQMQKKMAEAQEKLSSIEVEGVSGGGMVKVTAS
                     AKGDIKRIAIDDSLMKPEEKEITEDLIVAAINDAKQKGEAVSQEEMKSITGGISLPPG
                     MKLPF"
     gene            3018..3617
                     /gene="recR"
                     /locus_tag="EYO86_00520"
     CDS             3018..3617
                     /gene="recR"
                     /locus_tag="EYO86_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HIC41554.1"
                     /translation="MASSEIDKLINEVSKLPGLGRRSAQRIALYLLKNKERSLMPLVH
                     TLNEANEKIINCKVCGNIDMIDPCSLCQNTKRDEKTILVVEDVSDLWTFDRIGFFRGM
                     YHVLGGSLSAMNGIGTDQLTIKKLMQRIEKQQVTEVILALSTTMEGQTTSHVVADKLE
                     QFDNLTVTRLAQGIPIGGEVHFLDENTLSTAFQSRKKIV"
     gene            3625..>3816
                     /locus_tag="EYO86_00525"
     CDS             3625..>3816
                     /locus_tag="EYO86_00525"
                     /inference="COORDINATES: protein motif:HMM:PF01327.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIC41555.1"
                     /translation="MDKILFVPHPKLRQQAKTIIKVTQSEIDLSKKMLDVMLSAPGVG
                     LAANQIGILKKIVTVRIHDE"
BASE COUNT         1382 a          552 c          747 g         1135 t
ORIGIN      
        1 ggtgcttata gtgaattagc aggaaaaaat atatatcctg attcaaaatc tattccttgt
       61 aagacatttg aagaaatgtt tgagagtgtt cgagataaaa aagcaaatat tgccatagtt
      121 cctattgaaa attctagatc tggaagggtt ggtgatactc aacgactcat ccctgaatct
      181 caactaaaaa ttgttggtga atatttttta gaagtaaacc attgtttgat gggagtgccc
      241 ggatcaacat taaaggatat taagcgaatc tatagtcatg aacagggtat tgctcaatgt
      301 cgtaaaaaaa taattaataa taaaaaagat atggttattg ttgctgacac agcaggagca
      361 gcaaaaatga tatcagaaac aaatagcaaa gaagatgccg ctatagcatc aaatcttgct
      421 gctaaaattt ataacctaga tattcttgaa tcaagttttc aagattttga aaacaatgtg
      481 acacgatttt tgattatgtc taatgagctc aacattccaa aagttaatga gatagactta
      541 atgacaactt tagtttttga ggtaagaagt attccagccg ctttatataa gtctctaggt
      601 ggcttagcga gtaacggagt taatatgacc aaacttgaaa gttatattaa ccctcaaggg
      661 tttgatgtag ctcagtttta tatggatatt gagggtcatc ctaataatcg caatgtgaag
      721 cttgctcttg aggaaatgaa atttttttgt aagcacattg atattttagg tgtttataaa
      781 atgtcagatt ttagaaaaaa ataatcaaat aattgtaagt cagagtaggt ctttcttggc
      841 aataatgtta tattatgaaa ggaaggttga gagggcaatt aacttgctaa ttcagtcagg
      901 tctgaaagga agcagctgta ccaagcttaa ttgggtctct taaaccttcc aatttattaa
      961 aatatgatag ataaaattga gcaggaagaa tttagagttt tagctagaaa atatagacct
     1021 caggtttttg acgatctgat tggtcaagag actcttgttc gcacattaac taatgctatc
     1081 accaataatc gcattgctca tgcatatctt ttaaccggag tacggggcgt cggaaaaact
     1141 acgacagcac gcataattgc gatgagttta aattgtgaag atcgaattca agaaaaatgc
     1201 gagccatgtg caaaatgtga ttcatgtgta tctattcgag aagatcgaaa tttagatgtc
     1261 atagaaatgg acgctgctag taaaacgggt gttgatgata ttcgggaaat tattgataat
     1321 gtaaaataca aacctgttaa cagcaaatat aagattttta tcattgatga agtacacatg
     1381 ttgtccaaaa atgcgtttaa tgctcttctg aaaactctag aggagccacc tgcgcatgta
     1441 aaatttattt ttgcaacaac tgaagtaaag aaaataccca taacaataat ctcacgttgc
     1501 cagcgatttg atctacatcg tattgcaact acagtcttaa catcgcattt aatgaattta
     1561 gcaaaaaagg aaaacattaa tatagataag gatgcgatgg ccctgattgt tcgttcagct
     1621 gacggatcgg tccgagacgg tttgagttta ctcgatcagg cgattactaa cgaaaaagac
     1681 aaaataacag ctaattctat aattgatatg ttgggtttag cggatagagg aaatattttt
     1741 gatttaatgg atgctatttt tcagggcgac ccagtgaatg cattggcaat ctttaatcgt
     1801 attcatcaag cgggagcaga tgttgtaatg atttttgatg aaatgttgaa tgtcactcat
     1861 ttcatcactc agataaaaat agcacctgaa ttaaaagaag atattcatat tccagaatta
     1921 gagcgcaata agggttttga gatggcagag aaaatatcta tggcatcctt agggatgatt
     1981 tggcaggtat tattcaaggg atacgaagaa ttgcaatcag gctttcattt atttcagcaa
     2041 ggagagatga ttattattcg tcttatttat ttatatgacg gaccttcccc aggtgatctc
     2101 attaaaaaaa ttgaagaaga taaaaaatta tctttaaata tgagtacatc atctgaatct
     2161 tcttcatctg ttgaaaaaga tccaattatt aatcaagtaa gaaataaaaa caatgaaatt
     2221 aaagagaaga gagaggcaaa caaggaagat cttccaaatc caataggagt gttgacaatc
     2281 aagtcttatc gtcattttgt tgatttattt tatcaaaaaa aagaaggaat gttgcatact
     2341 tacttataca acaatgccaa acttgtttct tttaaagagg gtgaagtggt agtgaatgct
     2401 aaatctattg ctgacccgca ttttcctcgt acaattgcaa aactcgtttc tacatggaca
     2461 ggcagaatat ggcaggttag cacatcagca agcaatgttg gtaaaacact taatgaagaa
     2521 gatttgattc aacaacaaaa agaaatcgaa attatgaaaa atgaaccgga ggttaaagct
     2581 atattaggaa aatttcctgg tgtcactatt cattccatta cagatattca tcaaacggct
     2641 gatgaaaaag tatttgcaac taaaaataaa caaacaaagg agaaataatg gtcaatattg
     2701 gcaatatgat gaaacaagca caacaaatgc aaaaaaaaat ggctgaagca caagaaaaat
     2761 tgagctctat agaagtagaa ggggtttctg gcgggggtat ggttaaagta accgctagtg
     2821 ctaagggtga tattaagcgt attgctattg atgattcttt aatgaaacct gaagagaaag
     2881 aaataaccga agatctgata gttgcagcca ttaatgatgc aaaacaaaaa ggagaggctg
     2941 tatcacaaga agaaatgaag tcgataacag gtggcatttc tttgcctcct ggcatgaaac
     3001 ttccttttta gtaatcaatg gcaagttcgg aaattgataa actaattaat gaagtttcaa
     3061 aacttccagg attaggcaga cgatcagctc aaagaattgc tttatattta ctcaagaata
     3121 aggaaagatc attgatgcct cttgttcata cactgaatga agcaaatgaa aaaattatta
     3181 attgtaaggt atgtggcaat attgatatga ttgatccgtg ctcattatgt caaaatacaa
     3241 aacgggatga aaagactatt ttagtcgttg aagatgtttc agatttatgg acatttgatc
     3301 gaataggttt ctttcgtggt atgtaccatg tgcttggagg gtcactttct gcaatgaatg
     3361 gaattggcac agatcaatta acaataaaga aattaatgca acgtattgag aaacaacaag
     3421 tgacagaagt gattttagca ctaagcacta caatggaagg gcaaacaaca agtcatgtag
     3481 tagctgataa actagagcaa tttgataatt taaccgttac tcgtcttgct caaggcattc
     3541 caattggcgg agaagttcat ttcttggatg aaaatacttt aagtacagct tttcaatcac
     3601 gtaaaaaaat cgtttagatt ccttatggat aaaattcttt ttgttcctca cccaaaattg
     3661 cgtcaacagg caaaaacaat aattaaggtg acacaatcag aaattgattt atcaaaaaaa
     3721 atgctagatg ttatgctaag tgccccaggt gttggattgg ctgctaatca aataggaatt
     3781 ttaaaaaaaa ttgttactgt tcgtatccac gatgaa
//
LOCUS       DTVV01000038            1644 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1130146, whole genome shotgun sequence.
ACCESSION   DTVV01000038 DTVV01000000
VERSION     DTVV01000038.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1130146"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /gene="ilvC"
                     /locus_tag="EYO86_00530"
     CDS             <1..956
                     /gene="ilvC"
                     /locus_tag="EYO86_00530"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIC41556.1"
                     /translation="VSILGFGSQGHAHAMNLKDSGVKNIVVGLRNNSSSVEKAKKADF
                     QVLSPEKAIEYGEVVMILIPDELQPDLYKKIIKKKIVNNAYLGFAHGFSIHFKFIKPR
                     KDLNIFMVAPKGPGHLVRSEFLNKRGVPCLLAIDKAISNNTKSLGLSYASAIGGGRTG
                     IIETSFREECETDLFGEQVVLCGGLTSLIKSGFETLVKSGYKPEMAYFECLHEIKLIV
                     DLIYEGGIANMRYSISNTAEYGDFTRGPRIIDKKVQKEMKKILKEIQSGKFAKEWMKE
                     NKEGRKKFKKYRKLGQNHEIESIGNKLRKMMPWIGKNKIVR"
     gene            1019..>1644
                     /locus_tag="EYO86_00535"
     CDS             1019..>1644
                     /locus_tag="EYO86_00535"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954895.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIC41557.1"
                     /translation="MGNYIRIFDTTLRDGEQSPGASMFVEDKIKIAKTLDIMGVDIIE
                     VGFPVASKGDFESIELVSQVVKKSIVCALARAKKKDIEVAAKSIIKAKQSRIHTFIAT
                     SKLHMEYKLKLKKEQVLEHIEASVKLARKFTDDVEWSCEDASRSDRPFLFKCIELAIK
                     SGANTINIPDTVGYAVPFEFGGLIKDIKNNVKNIDQIIISVHCHNDLG"
BASE COUNT          633 a          194 c          315 g          502 t
ORIGIN      
        1 aagtatcaat attaggtttt ggaagtcaag gtcacgcaca tgctatgaat cttaaagatt
       61 ctggcgtaaa aaatattgtc gtaggattaa gaaataattc ttcgtcagta gaaaaagcaa
      121 aaaaagctga ttttcaagtc ctttcaccgg aaaaagctat tgagtacgga gaagtagtta
      181 tgattttaat tcctgacgaa ttacaaccag atttatataa aaaaattata aagaaaaaaa
      241 tagttaataa tgcatatttg ggttttgctc atggatttag tattcatttt aagtttataa
      301 agccaagaaa agatttgaat atttttatgg ttgctccaaa aggccctggt catttggtca
      361 ggtctgaatt tttaaataaa agaggagtgc cttgcttatt agcaatagat aaagctataa
      421 gtaataatac caaaagtctc ggattatctt atgcatctgc tattggtggt ggtcgaacag
      481 gtattataga aacatcattt cgagaagaat gtgaaactga tctttttgga gaacaagttg
      541 tattgtgtgg tggccttaca tcgttaatta aatctggttt tgaaacttta gttaaatcag
      601 gatacaaacc agagatggct tattttgaat gtttacatga gataaaatta atagttgatt
      661 taatttatga aggtggcata gcaaatatga gatattccat ttcaaatact gctgaatacg
      721 gtgattttac aaggggtcca agaattattg ataagaaagt tcaaaaagaa atgaaaaaaa
      781 ttttaaaaga aatccaaagt ggtaaatttg ctaaagagtg gatgaaagaa aacaaagaag
      841 gtagaaaaaa atttaaaaaa tatagaaaat tggggcaaaa tcatgagata gaatctatag
      901 gaaacaaatt aagaaagatg atgccttgga ttggaaaaaa taagattgtc cgataatgct
      961 tcattgatga agttatcttt gacattatat atactccaag ttattttaat tgaaagcaat
     1021 gggcaattat ataaggatat ttgatacaac attaagagac ggagaacaat ctccaggcgc
     1081 ttcaatgttt gtagaagata aaattaaaat agcaaaaact ctggatataa tgggtgttga
     1141 tattatagaa gtgggatttc ccgttgcttc aaaaggtgat tttgagtcta tagaacttgt
     1201 atcgcaagta gtaaaaaaat caattgtttg tgctcttgct agagcaaaaa agaaagacat
     1261 cgaggtcgct gccaaatcaa tcattaaagc taaacaatca agaatacata cgtttattgc
     1321 aactagcaaa ctccatatgg aatacaaatt aaaattaaag aaggaacaag ttttggaaca
     1381 tatagaagca agtgttaaac ttgcaagaaa gtttacagat gatgtagaat ggtcttgtga
     1441 agatgcatca aggtctgata ggccattctt atttaaatgt attgagttag caattaaatc
     1501 tggagccaac acgattaaca ttccagacac cgtagggtac gctgtgccat ttgagtttgg
     1561 tggattaatc aaagatatta aaaacaatgt caaaaatatt gatcaaatta taatttcagt
     1621 acattgtcat aatgatttag gatt
//
LOCUS       DTVV01000039            1370 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1130149, whole genome shotgun sequence.
ACCESSION   DTVV01000039 DTVV01000000
VERSION     DTVV01000039.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1130149"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(41..128)
                     /locus_tag="EYO86_00540"
     tRNA            complement(41..128)
                     /locus_tag="EYO86_00540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(92..94),aa:Leu,seq:gag)
     gene            176..841
                     /gene="lipB"
                     /locus_tag="EYO86_00545"
     CDS             176..841
                     /gene="lipB"
                     /locus_tag="EYO86_00545"
                     /inference="COORDINATES: protein motif:HMM:PRK14341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIC41558.1"
                     /translation="MKKIYTKENIEIITSNELINYAEAIKFMEDRVRKIICNKDRELI
                     WFLNHDHMYTTGTSSDENEILTHSNVPIIKTNRGGKTTYHGPGQRIVYFLINLNNRKK
                     DVRKFIDLISISAIKLLANLDIQATTYPDRVGIWVTRTKNSSLLKEKKIGAIGLRIKK
                     WVTYHGLSFNINPKLKFYKNINACGLKNYESTSTEDLESNLSQDNFDKMYLQNFLKEL
                     SNV"
     gene            complement(827..>1370)
                     /locus_tag="EYO86_00550"
     CDS             complement(827..>1370)
                     /locus_tag="EYO86_00550"
                     /inference="COORDINATES: protein motif:HMM:PF00849.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HIC41559.1"
                     /translation="SDKYNLVHRLDRETSGLLIIAKNRQSTKIFGKLFKDHKISKVYL
                     AICQGHPKNPESEINLSIAAKNKNNKKSNSITRYKVLQTKNKLSNILFVPKTGKTHQI
                     RIVSQHLGCPIVGDTKYNKQNKYNFEKLKLNAHMLQFYMNDNKYEFTSKLPTDFLNFF
                     RNNNLKLVSGKELKTFLNIA"
BASE COUNT          514 a          180 c          209 g          467 t
ORIGIN      
        1 atgatctgta ttgttttgaa tattcataaa aaaaactaat tggtgcggct gagaagagtt
       61 gaacttccac gggattaaat cccacagcga cctcaacgct gcgcgtatac cagttccgcc
      121 acagccgcag aattaataga gtttacctat cagataggat attaagtatc aaccaatgaa
      181 aaaaatttac acaaaagaaa atatagaaat aataacatca aatgaattaa ttaattatgc
      241 agaagcaata aaatttatgg aggacagagt aagaaaaata atatgtaata aggatagaga
      301 actaatttgg tttcttaatc atgatcatat gtacacaact ggcacaagta gtgatgaaaa
      361 tgaaattcta acacactcga atgtgcccat cattaaaaca aatagagggg gaaaaactac
      421 ttatcatggc ccgggtcaac gaattgttta ctttctaatc aatttaaata atcgaaaaaa
      481 agatgttcgc aaattcattg atttaatatc aatatcagca attaaacttc ttgcaaactt
      541 agacatacaa gcaactactt atcctgatag agttggtatt tgggttacta gaaccaagaa
      601 cagttcattg ttaaaagaaa aaaaaattgg agctattggg ttaagaatta aaaaatgggt
      661 aacctatcat ggattaagct ttaatataaa tcctaaatta aaattttata agaatattaa
      721 tgcttgtggt cttaaaaatt atgaatccac atctacagaa gatttagaat caaatttatc
      781 ccaagataat tttgataaaa tgtatttaca aaattttttg aaagaattaa gcaatgttta
      841 aaaaagtttt taattctttt ccagaaacta actttaaatt gttattacga aaaaagttta
      901 aaaaatctgt tggcaactta gaagtaaact catatttgtt atcattcata taaaattgta
      961 gcatatgtgc attcaatttt agtttctcaa aattgtattt attttgttta ttgtatttag
     1021 tatcgccaac aattggacaa cctaaatgct gtgaaacaat tcttatttga tgtgttttac
     1081 cagttttagg aacaaataat atattgctta atttattttt tgtttgcaaa actttgtaac
     1141 gagtaataga atttgatttc ttgttatttt tgttctttgc agcaattgaa aggttaattt
     1201 ctgattctgg attctttggg tgcccctggc atattgcaag gtatacttta ctaattttat
     1261 gatctttaaa tagtttgcca aaaattttgg tagattgacg atttttagca ataattaata
     1321 aacctgatgt ttcgcggtct agacgatgaa ctaaattgta tttatcagaa
//
LOCUS       DTVV01000040            1270 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1130405, whole genome shotgun sequence.
ACCESSION   DTVV01000040 DTVV01000000
VERSION     DTVV01000040.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1130405"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1270
                     /locus_tag="EYO86_00555"
     CDS             <1..>1270
                     /locus_tag="EYO86_00555"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970731.1"
                     /note="catalyzes the dehydration of
                     2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate
                     in valine and isoleucine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIC41560.1"
                     /translation="MARLNLPSIFMYGGSILPGKFKGKQVTVQDIFEAVGEYASGKIK
                     KEDLYALEKVACPSAGSCGGQFTANTMACVAEAIGLALPGTTAIPATFKEREKTAKLA
                     GKTIMQLIKNGPKPRDIITKKSLENATRVVAASGGSTNAALHLPAIAHEAGIDFDLFD
                     VMKVFKTTPYIASLKPSGKYVAKDLYDVGGVPVLMKALLDGGYLHKNCITVTGKTIAQ
                     NLKNFKFPKNQDVVQETKNPISKSSGVVVLKGSLASQGAVVKIAGMENLEFIGKARCF
                     DCEEDAFKAVQKKKYKAGDVIVIRYEGPRGGPGMREMLSTTAAIYGQGMGDKVALITD
                     GRFSGATRGFCVGHVGPEAAVGGPIALIKNGDKIHLDGNKGIINLLVNKNELAKRKKK
                     WKARKNYFQSGSLWRYAKNVGDAYKGAVTHP"
BASE COUNT          475 a          183 c          274 g          338 t
ORIGIN      
        1 atggccagat tgaatctgcc ttcaattttt atgtatggag gatcaatctt gcctggaaaa
       61 tttaaaggaa aacaggtaac tgttcaagat atattcgaag ctgttggtga atatgcttct
      121 ggaaaaataa aaaaagaaga tttatatgca ttggaaaaag ttgcctgtcc atctgcaggt
      181 tcatgcgggg gtcagtttac tgcaaacaca atggcgtgcg ttgcagaagc aattggacta
      241 gctctacctg gcaccactgc aataccagca acttttaaag aaagagaaaa aactgcaaaa
      301 ctagctggca aaaccattat gcagttaatt aaaaatggtc ctaaaccaag agatattatt
      361 accaaaaaat cattagaaaa tgcaacaaga gttgttgcgg catcaggcgg atcaacaaat
      421 gcagctttgc atttaccggc tatagcacat gaagcaggaa ttgattttga tttatttgat
      481 gtaatgaaag tgtttaaaac cactccttat attgcaagtt taaaaccaag tggaaaatat
      541 gttgctaaag atttatacga tgtaggagga gttcctgttt taatgaaggc attactagat
      601 ggtggatatt tacataagaa ttgcataact gttacaggaa aaacaatagc gcagaattta
      661 aaaaacttta aatttcctaa aaaccaagat gtggttcaag aaacaaaaaa cccaattagt
      721 aaaagtagtg gagttgttgt gctaaaagga tctcttgcat cacaaggcgc agttgtaaag
      781 attgcaggta tggaaaattt agaatttatt ggcaaagcta gatgctttga ttgtgaagaa
      841 gatgcattta aagcagtgca aaagaaaaaa tataaagcag gtgatgtaat agtaatacgc
      901 tatgaaggac cacgaggagg acctggtatg agagaaatgc tatctacaac tgcagccata
      961 tatggtcaag gtatgggaga taaagttgct ttgatcactg atggaagatt ttcaggtgct
     1021 accagaggat tttgtgtagg ccatgttgga ccagaagcag cagtaggtgg cccaatcgca
     1081 cttataaaaa acggagataa gattcattta gatggtaata aaggaataat aaatttatta
     1141 gttaataaaa acgaactagc taaaagaaaa aagaaatgga aagctagaaa aaattatttt
     1201 caatccggat ctctttggag atacgcaaaa aatgttggag atgcctacaa aggtgcagta
     1261 acacatcctg
//
LOCUS       DTVV01000041            1013 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1131623, whole genome shotgun sequence.
ACCESSION   DTVV01000041 DTVV01000000
VERSION     DTVV01000041.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1131623"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1013
                     /locus_tag="EYO86_00560"
     CDS             <1..>1013
                     /locus_tag="EYO86_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036760.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HIC41561.1"
                     /translation="ADTEKVKKQIEDVIGIDTNHSINCSGKTGEGVDDILESIVKNLP
                     SPKGNINNPLKSLLVDSWYDSYLGVVILVRVIDGSLKKGMKVKMMSNNTEHTIEQVGV
                     FTPKPQDIDKISSGEIGFIITGIKNLSDTKVGDTICDVSDPIDKPLPGFKPSKPVVFC
                     GLFPVDNSEHQKLKDGLAKLKLNDSSFSYEPEASSALGLGFRCGFLGLLHLEITTQRL
                     EREFNIALITTTPGVIYKINNRNGSVKELQNPTDMPDPTQILSIEEPWIKAIIITPDQ
                     YLGSVIKICQEKRGSQKNLTYSGNRAVLSYELPLNEVVFDFYDRIKSVSSGYASFDYE
                     ITG"
BASE COUNT          376 a          135 c          188 g          314 t
ORIGIN      
        1 gcagatacag aaaaggtaaa aaaacaaata gaagatgtaa ttggaataga taccaatcat
       61 tcaattaatt gttctggaaa aacaggagaa ggagtcgatg atattctaga atctatagtc
      121 aaaaatttac cttcccctaa aggaaacatt aataatccac taaaatcttt attggtagat
      181 agctggtacg atagttattt aggcgtcgtt attttggtaa gagttataga tggtagttta
      241 aaaaaaggta tgaaagttaa aatgatgtcg aataatactg agcataccat tgaacaagtt
      301 ggtgtattta ccccaaaacc tcaagatata gataaaattt catccggaga aattggtttt
      361 ataattacag gaataaaaaa tttatccgat actaaagtgg gagacacaat atgtgatgta
      421 tcagatccaa ttgataaacc actaccagga tttaaaccaa gcaagccagt tgtattttgt
      481 ggtctttttc cagtggataa ttcagagcat caaaagttaa aagatggttt agcaaaacta
      541 aaacttaatg attctagttt ttcttatgaa cctgaagctt caagcgcgtt aggtttagga
      601 tttcgatgtg gttttttggg tttacttcat ttagagatta caacacaaag attagaaaga
      661 gaatttaata ttgctttaat tactacgact cctggggtga tatataaaat taataacaga
      721 aacggatctg taaaggagtt gcaaaatcca actgatatgc ctgacccaac tcaaatttta
      781 tcaatagaag aaccatggat taaagctata attattactc cagatcaata tctgggctct
      841 gtaattaaaa tatgtcagga aaaaagagga tctcaaaaaa acctaactta ttctggaaat
      901 cgagcggttt tgagttatga gctgccatta aatgaagtgg tttttgattt ttatgatcga
      961 attaaatctg tttcaagtgg atatgcgagt tttgattatg agatcacagg ata
//
LOCUS       DTVV01000042            1094 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1132328, whole genome shotgun sequence.
ACCESSION   DTVV01000042 DTVV01000000
VERSION     DTVV01000042.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1132328"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="EYO86_00565"
     CDS             <1..344
                     /locus_tag="EYO86_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386490.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIC41562.1"
                     /translation="EMAAFAQFASDLDASTRQLLDRGVRLVELLKQGQYSPLTVPEQV
                     VSIYAGVNGYLDKLDVKDVVRFESEIHSEIRASHGDLLEAIDNEKELSSANEEKLKGF
                     LNTFVKKFQSN"
     gene            348..>1094
                     /gene="atpG"
                     /locus_tag="EYO86_00570"
     CDS             348..>1094
                     /gene="atpG"
                     /locus_tag="EYO86_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIC41563.1"
                     /translation="MPSLKDIKNKIKSVGSTKKITSAMKMVAASKLRRSQEKAEAARP
                     YSSRLEEMLSSLASSGTSGEGVIQLLTGTGKDERYLIVPVTADRGLCGGFNSSINRET
                     FKFVKSLEGEGKSVQLLTVGKKSRDFFNRIMKDKIVESFADFGSTGAGYDIALEISNK
                     LQNFYFDGQFDKCIIVYNKYVSAITQEVTQQQLIPLDISQPKKISNEKDEKDNSIYIY
                     EPDEETILKDLLPKNVSIQIFKVLLESNAGE"
BASE COUNT          380 a          165 c          215 g          334 t
ORIGIN      
        1 gtgaaatggc tgcttttgcc caattcgctt ctgatcttga tgcttctacg cgccagcttc
       61 ttgatcgcgg cgttcgcttg gttgaacttt taaaacaagg tcaatattcc ccactcactg
      121 ttcctgaaca agttgtttca atttatgcag gtgtaaacgg atacttggat aaactagatg
      181 tgaaagatgt tgttcgattt gaatcagaaa tacattctga aattagagct agccacggag
      241 atcttttaga agcaattgat aatgaaaaag aattatcatc tgcgaacgaa gaaaagttaa
      301 aaggcttctt aaatacattt gtgaagaaat ttcaaagcaa ctaatttatg ccaagtttaa
      361 aagatattaa aaataaaatc aaatctgttg gttcaacaaa aaaaattact tcagcaatga
      421 aaatggtagc ggcaagtaaa ttgcgacgtt ctcaagaaaa agcagaagct gcgcgaccct
      481 actcttcgcg tttggaagaa atgttatctt cactagcttc ttcaggcact tctggggagg
      541 gagttataca attgttaaca ggcacaggca aagatgaacg ttatctcatt gttccagtta
      601 cagctgatag agggttatgt ggtggtttta acagcagtat taaccgtgaa acatttaagt
      661 ttgtgaaatc tcttgaaggt gaaggtaaat ccgttcagtt attaaccgtt ggtaaaaaaa
      721 gtagagattt tttcaatcga attatgaaag acaaaattgt agaaagtttt gcagattttg
      781 gatctactgg agcagggtac gatattgctc tggaaatttc aaataaatta caaaactttt
      841 attttgatgg gcagtttgat aagtgtatta tcgtgtataa taagtatgta tcagctatta
      901 cgcaagaagt aacacagcaa cagcttattc cattagatat atctcagcct aaaaaaatat
      961 caaatgaaaa ggatgaaaaa gataactcta tctatattta tgagcctgac gaagagacta
     1021 ttttgaaaga tttattgcca aagaatgtat ccattcaaat ttttaaagtg ttattagaaa
     1081 gtaacgcagg cgaa
//
LOCUS       DTVV01000043            1409 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1133447, whole genome shotgun sequence.
ACCESSION   DTVV01000043 DTVV01000000
VERSION     DTVV01000043.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1133447"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..98)
                     /locus_tag="EYO86_00575"
                     /pseudo
     CDS             complement(1..98)
                     /locus_tag="EYO86_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
     gene            90..656
                     /gene="fusA"
                     /locus_tag="EYO86_00580"
                     /pseudo
     CDS             90..656
                     /gene="fusA"
                     /locus_tag="EYO86_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725633.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            656..964
                     /gene="rpsJ"
                     /locus_tag="EYO86_00585"
     CDS             656..964
                     /gene="rpsJ"
                     /locus_tag="EYO86_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIC41564.1"
                     /translation="MESQDIRIRLRAYDNKLLDISAADIINTAKRTGAKVRGPIPLPT
                     KMEKFTVNKSPHVDKKSRDQLEIRTHNRLLDIIDPTPQTIDALMKLDLSAGVEVDIKI
                     "
     gene            966..>1409
                     /gene="rplC"
                     /locus_tag="EYO86_00590"
     CDS             966..>1409
                     /gene="rplC"
                     /locus_tag="EYO86_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIC41565.1"
                     /translation="MRTGIIGTKIGNTSFFNEDGSVSPVTLVKIEECVVSGVKTKDKD
                     GYFAIQLASIDNKSKNPKIKKPQKKIFASLNIKPKKIVKEFRVIEENLLKIGTSINAG
                     HFEKEQKIDVTGTSIGKGFAGVMKRHNFSGQRASHGVSLSHRKAGS"
BASE COUNT          513 a          208 c          295 g          393 t
ORIGIN      
        1 agataaacct cctggtacac ccggtcgatc agggctgcct tggtcgggaa atagcggaac
       61 agattctggt gggtaatgcc taggcgcagg gccagttcgc aagagttaag atttcttttg
      121 atccaggtga gccaggaagt ggatatgagt ttattaataa aattaaagga ggaaacatac
      181 caactgatta cattccaggc gtcaaaaaag gtttgattgc ccaacaacaa tctggagtta
      241 ttgcaggctt tccttgtatt gattttaaag caactttaaa tgatggtgcg tatcatgatg
      301 tagattcaag tgttctagcg tttgaaatcg ctgcaagggc agcatttaga gaaggtatcg
      361 caaaagcaaa tccagtttta ttggaaccga ttatgaaggt agaagttctt acaccagaaa
      421 agtatatggg tgacgtaatt ggtgatttaa atagtcgccg cggtcaagtt caagagatga
      481 atactagagg gaatgctaat gtagtcaatg caatggtacc tctcgccaac atgtttggtt
      541 atgtaaacaa tttaagatct ttaactcaag gaagggcaaa ttattctatg caatttgatc
      601 attatgagca agtaccttcc aatgtagctg aagaagtaaa ggcaaaatta gcataatgga
      661 atctcaagat attagaatca gattaagagc ttatgacaat aagcttcttg atataagtgc
      721 tgcagatatt atcaacacag caaaaagaac aggggcgaaa gtgagaggcc ccattccctt
      781 accaacaaaa atggaaaaat ttacagttaa taaatctcca catgttgata agaaaagtag
      841 agatcaatta gaaattagaa ctcataacag actattggat attatagatc caacacccca
      901 aacaattgat gccctaatga aattagattt atcagctgga gttgaagtgg atataaaaat
      961 ttagtatgag aacgggaatt ataggtacaa aaattggaaa tacttcattt tttaatgaag
     1021 atggttctgt atctccagtt acattggtta aaattgaaga atgtgttgtt tctggagtga
     1081 aaacaaaaga caaggatggt tattttgcaa ttcagcttgc aagtattgac aataaatcta
     1141 aaaatccaaa aataaagaaa cctcaaaaga agatatttgc gtctttaaat attaagccaa
     1201 aaaaaattgt taaagaattc cgtgtaattg aagaaaattt attaaaaatt ggcacttcaa
     1261 tcaacgcagg gcattttgaa aaagagcaaa aaattgatgt tacaggaaca agtataggta
     1321 aaggttttgc tggagtaatg aagcgtcata attttagtgg tcaaagagca tcccatggtg
     1381 tttctctttc acacagaaaa gctggatca
//
LOCUS       DTVV01000044            3409 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1138875, whole genome shotgun sequence.
ACCESSION   DTVV01000044 DTVV01000000
VERSION     DTVV01000044.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1138875"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="EYO86_00595"
     CDS             <1..1411
                     /locus_tag="EYO86_00595"
                     /inference="COORDINATES: protein motif:HMM:PF01019.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIC41566.1"
                     /translation="GIGGDCFALVSIKGKKSIAVNGSGIAPKKATLDFFESSKINKIS
                     TTSPHSVNIPGAIHAWHSMHQKFGSLDFAELLISAEKYAREGFPVHEVVAKAWSENID
                     KLQQHPVTKAIFLRNNKSYGFGEVHQNINLANTLQSIAKNGIKDFYSGYIAKDIVSSL
                     NKIGGLHSMDDFAEQDTIFSDSLTNNYRDIILHQCPPNGPGITVLLMMGILEKFDFTK
                     IKSLSADRFHLQAEATKIAFEIREGSIGDPKFNDIDFHQLIQAEYINELASKISMDKI
                     YKPKNSTITAHPETVYLTVVDKDQNAVSFINSICFPFGSGITSNKTGVILQDRCVNFR
                     LERDHPNMIDGHKRPLHTIIPGQVTDMDNNPLLSYGVMGGQYQPVGHSHVLQNIFDFG
                     MSVQEAIDFPRAFILENDYQFEKSIPEDILLDLKSRGHQISYSDHTHGGGQAILIDRK
                     NGVLIGGSDPRKDGCAIGY"
     gene            complement(1408..2445)
                     /locus_tag="EYO86_00600"
     CDS             complement(1408..2445)
                     /locus_tag="EYO86_00600"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIC41567.1"
                     /translation="MRIEEEIKLDYSDVLFRPKRSTLKSRKEVDLCRTYKFKHSNREW
                     TGIPIIAANMDGVGELNVASSLAKYKIITALTKQHNLDLINNFSPIKDIFNYIALSSG
                     TGKESFERLKQIISEHTYIQFICIDVANGYSENFSHFVSSVREKYPSKTIIAGNVVTA
                     DMTQELILSGADVVKVGIGPGSVCTTRIQTGVGYPQLSAVIECADAAHGLGAHIIADG
                     GCTCPGDVAKAFGAGADFVMLGGMLAGHEEGGGKIISENEKQYIEFYGSSSEEAIEKH
                     YGGLSDYRSSEGKKVKIPFRGKLDQSVKAILGGVRSSCTYVGAPSLKQLSKCTTFIRV
                     NQQYNDTFGRV"
     gene            2528..3409
                     /locus_tag="EYO86_00605"
     CDS             2528..3409
                     /locus_tag="EYO86_00605"
                     /inference="COORDINATES: protein motif:HMM:PF00348.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIC41568.1"
                     /translation="MKINTLINGERKNFENYFVKLLKSTLSREILSKAMFYGSMNGGK
                     RIRPFIVSIFAKLANVKKQHYLRLSAAIECIHSYSLIHDDLPSMDDDDFRRGKLSTHK
                     KFNEATAILAGDALHDLAFEIIANKKTHQDAKIRIQLIEKLSKILGFQGLAGGQSCDL
                     LFEKTKIKKKDILKMYKLKTSALFSFCCSSAFIVGGKNNKEIKFAEEFGSIFGLMFQI
                     IDDILDETKDFKFLGKTPGKDKKQGKSTLISVIGEEKAIKFCLHEIKQFEYKNKKYFN
                     QYKILTEVLSMNLKNYK"
BASE COUNT         1199 a          544 c          551 g         1115 t
ORIGIN      
        1 gggaattgga ggcgattgtt ttgctctggt ttctataaaa gggaaaaagt caatagcggt
       61 taatggttcg ggcatagctc ccaaaaaagc taccttggat ttttttgaaa gtagtaaaat
      121 taataaaatt tcaacaacaa gtcctcattc agtgaatatt ccgggggcaa ttcatgcttg
      181 gcattcaatg catcaaaagt tcggtagctt agattttgct gaattactta tatcagctga
      241 aaaatatgca agggaaggtt ttcctgttca tgaagttgtc gccaaagctt ggtctgaaaa
      301 catagataaa ttacaacaac atcctgtcac taaagcaata tttttacgga ataataaatc
      361 ttatggtttt ggagaagttc atcaaaatat aaatttagcc aacacacttc aatcaattgc
      421 taaaaatgga attaaagatt tttattcagg gtatattgca aaagatattg ttagttccct
      481 taataaaata ggcggattgc actcaatgga tgattttgca gagcaggata ctattttttc
      541 agacagccta acaaataatt acagagacat tatacttcat caatgtccgc ccaatgggcc
      601 gggtattacg gtgttattaa tgatgggtat tttagaaaaa tttgatttta cgaaaattaa
      661 atcactaagt gctgatagat ttcatttaca agcagaagca accaaaatag cgtttgagat
      721 aagagaaggt agtataggcg atccaaagtt caacgatatt gattttcatc aattaattca
      781 agctgaatat atcaatgaac tagcctcaaa aatatcaatg gataaaatct ataaaccaaa
      841 aaattctacc attacggctc acccagaaac agtttattta acagttgttg ataaagatca
      901 aaatgccgta tcctttatta attctatatg ctttccattt ggaagtggta tcacaagcaa
      961 taaaacaggt gttatacttc aagaccgttg tgtgaatttt cgtttagaaa gagatcatcc
     1021 taacatgatt gatggccata aaaggcctct ccacaccatt ataccaggac aggtaaccga
     1081 catggataat aatccattac tatcttatgg tgttatggga gggcaatatc aaccagtggg
     1141 tcatagtcat gtactgcaaa atatttttga ttttggaatg tctgttcaag aagctattga
     1201 ttttccaaga gcgtttatat tggaaaatga ctatcaattc gaaaaatcta taccagaaga
     1261 tattttacta gatttaaaat ctagaggtca tcaaattagc tactcggatc atacccacgg
     1321 aggtggacag gcaattttaa ttgatagaaa aaatggagta ttaattgggg ggtctgatcc
     1381 aagaaaagat ggttgtgcta tagggtatta aactctacca aaagtatcat tatattgctg
     1441 attaaccctg ataaaagttg tacatttact tagctgcttt aaagatggag ctcccacgta
     1501 tgtacagcta cttctaaccc ctcctaaaat agctttaacg gattgatcta atttacctct
     1561 aaatggaatt ttaacttttt ttccttcgga agatctataa tctgataaac ctccgtagtg
     1621 tttttcaatt gcttcctcgg aacttgaccc atagaattct atatattgct tttcattttc
     1681 agaaattatt ttacctccac cttcttcatg tccagccaac ataccgccaa gcataacaaa
     1741 atctgctcca gcaccgaatg cttttgctac atctcctgga caagtgcagc ctccatcagc
     1801 aattatatgt gcacctaaac catgtgctgc atctgcgcat tctattaccg cacttaattg
     1861 aggatagcca actcccgttt gaattcttgt tgtacaaacg cttcctggcc ctatgccaac
     1921 tttcactaca tctgctccac tcaaaattag ttcctgagtc atatctgcag taacaacatt
     1981 cccagcgata attgttttag aaggatattt ttctctaaca gaagatacaa aatgactaaa
     2041 gttttccgaa taaccattag ccacatcaat acaaataaac tgaatatagg tatgttcact
     2101 aataatttgt tttaatctct caaaactctc tttgcctgtt ccagaactaa gagcaatgta
     2161 attaaaaata tctttgatgg gactaaaatt gttaatcaaa tctaaattat gctgtttagt
     2221 taaggcggtt atgattttat attttgctaa actacttgct acattcaatt cccctacccc
     2281 atccatatta gctgcaataa tgggaatacc agtccattct ctattagaat gtttgaattt
     2341 gtaagttctg catagatcaa cttcttttct gctttttaat gtacttcttt tgggtctaaa
     2401 taaaacatcc gaatagtcta gtttaatttc ttcttcaatt cgcatagtag aaaatgtatt
     2461 ataagctatt aatatattga taattattta aattattttg ataatacaac aaccataaat
     2521 tcaaataatg aaaataaata ccttaataaa tggggaaaga aaaaattttg aaaattattt
     2581 tgtaaaatta ttgaaatcca ctttatctag agaaatactt tcaaaagcta tgttttatgg
     2641 atcaatgaac gggggaaaga ggattcgacc ttttattgtt agtatttttg ccaaattagc
     2701 taatgttaag aaacaacatt atttacgatt atctgctgcg atagaatgta ttcattcata
     2761 ctccttaatt catgacgact tacccagtat ggatgatgat gattttagga gaggtaaatt
     2821 aagtactcac aaaaaattta atgaagcaac ggcaatctta gcaggtgatg cgttgcacga
     2881 cttagcattt gaaattattg caaataaaaa aactcatcaa gatgctaaaa taagaattca
     2941 attaatagaa aaactctcaa aaatactagg ctttcagggt cttgctgggg gtcaatcttg
     3001 tgatttgttg tttgaaaaaa caaaaattaa aaaaaaggat attttaaaaa tgtataaatt
     3061 aaaaacatct gctcttttta gtttttgttg ttcatcagct tttattgtgg ggggaaagaa
     3121 taataaagag ataaaatttg cggaagaatt tggaagtata tttggtctta tgttccagat
     3181 aatagatgat attttggatg aaacaaaaga ttttaaattt ttaggtaaaa cccctggaaa
     3241 agataaaaaa caaggtaaaa gtactctcat ttctgtaata ggagaagaaa aggcaataaa
     3301 attttgtttg catgaaatta aacaatttga atataaaaac aaaaaatatt ttaatcaata
     3361 taagattttg actgaagttt tatctatgaa tttaaaaaat tacaaataa
//
LOCUS       DTVV01000045            1402 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1140762, whole genome shotgun sequence.
ACCESSION   DTVV01000045 DTVV01000000
VERSION     DTVV01000045.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1140762"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="EYO86_00610"
     CDS             complement(<1..111)
                     /locus_tag="EYO86_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIC41569.1"
                     /translation="MRKYYTRPCNFYYGNYARNLVRKKKAFLLAGNPNIAF"
     gene            complement(118..>1402)
                     /gene="hflB"
                     /locus_tag="EYO86_00615"
     CDS             complement(118..>1402)
                     /gene="hflB"
                     /locus_tag="EYO86_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIC41570.1"
                     /translation="LAKAIAGEASVPFFSISGSDFVEMFVGVGAARVRDMMEQGKKNA
                     PCIIFIDEIDAVGRSRGAGLGGGNDEREQTLNQLLAEMDGFETNEGVIVIAATNRPDV
                     LDPALLRPGRFDRQVVVPNPDIVGREAILKIHIKKINIGPDVKLRIIARGTPGFSGAD
                     LANLCNESALLAARKNKRIVTMSDIEEAKDKVMMGAERRSMVLTEDDKKLTAYHEGGH
                     AIVTLNEKASDPIHKATIIPRGRSLGVVFTLPERDKFSHTREYLEANISKAMGGRVAE
                     EMIFGHDKVTSGASSDIEMATKLAKDMVTKYGMSKDLGPLSYGANEDEIFLGRQITRQ
                     EHMSEETAKKVDSEVKKIVDAGYQRAKKILTEKIDDLHKLAKALLIYETLSGEEIKNL
                     ISKNIQPKTTTDEDDKNTEESSALGSLGLKPKPAH"
BASE COUNT          382 a          309 c          191 g          520 t
ORIGIN      
        1 aaaagctata tttggattgc ccgctaataa gaaagccttt ttttttctaa caagatttct
       61 tgcataattt ccgtagtaga aattacacgg tcttgtgtaa tattttctca ttagaattta
      121 atgtgcaggt tttggtttta aacctaaaga tcccaatgct gaagactcct ctgtattttt
      181 atcatcttca tcggttgttg tcttgggttg aatatttttt gatattagat tttttatttc
      241 ttctccgctt aaagtttcgt aaataagcaa agctttagcc aatttatgta aatcatctat
      301 cttctcggta agaattttct ttgctctttg gtagcccgca tcaacaattt ttttaacctc
      361 gctatcaact ttttttgctg tctcctctga catatgctct tgtctagtta tttgtcttcc
      421 gagaaaaatt tcgtcctcat tagctccata acttaatgga ccaaggtctt tactcattcc
      481 atattttgta accatatctt ttgctaattt agtggccatt tcaatgtcag aagaagctcc
      541 ggaagttact ttgtcgtgac caaaaatcat ttcttctgca actctaccgc ccatggcttt
      601 ggatatgttt gcttccaagt attctctggt atgagaaaat ttatctcttt ctggtaaggt
      661 aaaaaccaca cctaaggatc ttcctctcgg tataatcgta gctttgtgta taggatcgga
      721 agctttttca tttaaagtaa caattgcgtg accaccttcg tgatacgctg ttaatttctt
      781 atcatcttca gtcaaaacca ttgatcttct ctccgctccc atcattacct tatctttggc
      841 ttcctcaata tctgacattg ttacaattct tttatttttt ctagcagcca ataaagctga
      901 ctcattacat aaattggcta aatcggctcc agaaaaacca ggtgtgcctc tagcaattat
      961 ccttaatttt acatctggac caatattaat ttttttaata tggatcttta aaatagcttc
     1021 ccttccaaca atatcaggat ttggtacaac tacttgccta tcaaatcttc cgggtcttaa
     1081 taaagcagga tccaaaacat caggtctatt agttgctgca ataactatta ctccttcatt
     1141 agtttcaaaa ccatccatct ccgcaagcaa ttgatttaat gtttgttctc tttcatcgtt
     1201 tcctccacca agtcctgctc ctctacttct tccaactgca tcaatctcat ctataaaaat
     1261 tatacaagga gcattttttt ttccttgctc catcatatcc ctaacccttg cggcaccaac
     1321 accaacaaac atttcaacaa aatctgatcc tgaaatagaa aaaaaaggta cacttgcttc
     1381 acctgctatt gctttggcta ac
//
LOCUS       DTVV01000046            1904 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1143418, whole genome shotgun sequence.
ACCESSION   DTVV01000046 DTVV01000000
VERSION     DTVV01000046.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1143418"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="EYO86_00620"
     CDS             complement(<1..199)
                     /locus_tag="EYO86_00620"
                     /inference="COORDINATES: protein motif:HMM:PF01329.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIC41571.1"
                     /translation="MLLLSKDDIKKGLVSLYAWEYDNKSIRKTYSFNAYTDGIKFVNE
                     IAEISEKNNHHPDLYIGWRKVT"
     gene            complement(233..604)
                     /locus_tag="EYO86_00625"
     CDS             complement(233..604)
                     /locus_tag="EYO86_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HIC41572.1"
                     /translation="MKKIKSVNVFGKELKPCCNNPKTGFFRDGLCNTCAEDFGLHTVC
                     VLLTKEFLEFSKKAGNDLSTPRPEFDFPGLKPGDRWCLCAMRWKEANEHNAAPPLFLE
                     ATHQETLKVISLTELQQFVMN"
     gene            complement(764..1312)
                     /locus_tag="EYO86_00630"
     CDS             complement(764..1312)
                     /locus_tag="EYO86_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011636367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIC41573.1"
                     /translation="MKTAHNSALVIIDIQGNLAQLMHDKENLFKNMQLLIRGAQLLNI
                     PIIWTEQLPDKLGETTQEIGKLLSSQKPVVKDVFSCARNGEFNMQIEKIQPEHIMLAG
                     IETHICVYQTASDLLGEKYQVEIVADATSSRTQSNKKIGLEKIKHEGGCLTSVETLLF
                     EIQGTATGEQFRELIKLIKKFN"
     gene            complement(1348..1554)
                     /locus_tag="EYO86_00635"
     CDS             complement(1348..1554)
                     /locus_tag="EYO86_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41574.1"
                     /translation="MKKSEKNIETKKIIYGMNDVPSGTTIKIKDTGKEVKVIEVLNFP
                     TRYKCDDKNIYYVSQVIFFDWPPE"
BASE COUNT          561 a          337 c          288 g          718 t
ORIGIN      
        1 cagttacctt tcgccagcct atatataaat ctggatgatg gttatttttt tcggaaattt
       61 cagcaatttc attaacaaat tttatcccat ctgtgtatgc attaaatgaa tatgtttttc
      121 tgattgactt gttatcatat tcccaagcat aaagagatac taatcccttt ttaatgtcgt
      181 cttttgaaag taatagcatt tgttttcctt tctttgaatg aaacctcaat agttaattca
      241 taacgaattg ctgaagctct gttaaactaa taaccttcaa tgtttcctgg tgagttgctt
      301 ctaaaaataa cggcggcgct gcattatgtt catttgcttc tttccatcgc atggcacaga
      361 gacaccacct gtctcccggt tttaatcctg gaaaatcaaa ttccggtctt ggtgtcgata
      421 aatcgttccc tgctttttta ctgaactcta aaaattcttt tgtcaataaa acgcagactg
      481 tgtgtaggcc aaagtcttct gcacaggtat tacataatcc atcccgaaaa aaacccgttt
      541 tgggattatt gcagcagggt tttagttctt tcccaaagac atttactgat tttatctttt
      601 tcaatttgtt ttctactcct gttttccggg gttatttttt tcaagaatgt attttgtaat
      661 tttattgagt ttcctgacct cttttttcaa tttaaatatt tcagaaagaa ctaccatagc
      721 aaaaacaaca cttagcaaac tgattgtggt cataagtgtc atatcagtta aattttttaa
      781 tgagtttgat taattcccgg aattgttcac cagttgcggt accttggatt tcaaatagta
      841 gagtctccac acttgtcaaa caccctcctt catgttttat tttttctaat ccgatctttt
      901 tatttgattg tgtccgggaa gatgttgcat cagcaacaat ttcaacctga tatttttctc
      961 ccaataaatc cgatgcggtt tggtacacgc aaatatgtgt ttcgattcca gccaacataa
     1021 tatgctctgg ttgaattttt tcaatttgca tattgaactc accatttctc gcacaggaaa
     1081 acacgtcctt tacaacaggt ttttgggagg acaataattt cccgatttcc tgtgttgttt
     1141 ctcctaattt atcagggagt tgttctgtcc aaataattgg tatattcaat aactgtgctc
     1201 cgcgtataag caattgcata tttttaaaca aattttcctt gtcatgcatt agctgggcca
     1261 ggttaccttg aatgtcaatg ataactaaag cgctgttgtg tgctgttttc atgaatacag
     1321 aatcaaagca aatattttta cctttgatta ttcaggaggc caatcaaaaa aaataacttg
     1381 agaaacataa taaatatttt tgtcatcgca tttatacctg gttggaaaat taaggacttc
     1441 aattacttta acttcttttc ccgtgtcttt tatttttatt gttgtgccac ttgggacatc
     1501 attcattcca tatattattt tttttgtttc aatattcttt tcggattttt tcactgaaaa
     1561 tattccatga ttttcatttc aaattaataa acaaacacac caatagtttt acgaaaacat
     1621 tttgataaaa ttattgcatc ttttccggat ttgatctcca gaattaaatg gaaatggatt
     1681 cattgttcga gttagtagaa aataacaatc cgaaactcat tattatggat aatattttct
     1741 tcatgtatgg aaatttacgt taatctatgc gtaacaaaaa agcctgttaa atatcacaaa
     1801 caggtaatca aaatgttcct aaattattcc atttaaagga tgcatttaat aaatgattga
     1861 acaaaaaaaa tatgatctag tggtttgggg tgcaactggt ttta
//
LOCUS       DTVV01000047            1260 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1147312, whole genome shotgun sequence.
ACCESSION   DTVV01000047 DTVV01000000
VERSION     DTVV01000047.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1147312"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            51..>1260
                     /locus_tag="EYO86_00640"
     CDS             51..>1260
                     /locus_tag="EYO86_00640"
                     /inference="COORDINATES: protein motif:HMM:PF00528.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41575.1"
                     /translation="MFNYIIRRLLLMIPTFFGTTIMVFTILQYVPGGPFERAVMQLKF
                     ASMMSGESASAGVKSISDSQTLTPEILEDLRMQYGLDKPVVIRYLIWLGIWPKEIKNR
                     TVNIGETFRENIDSFKKDAFTSYTLQKWIKPVKNKTGEIIVTESGVGIDIKNADFEPT
                     KDFQELPNVNFIKTWYHSTNWKIAEIKSDSEIRIIQKSYQGLFTGYLGYSNEKADDVG
                     TLIKNRLHISLIFGLTSFILVYLICIPLGIMKAIKHGSKFDVISSSVVFIGYSIPGYA
                     LGTLLLVYFAGENFMDIFPLGGWRSENWNNLTFMGKVFDQIHHAILPILCYMVGSFAT
                     LTVLMKNSLMENLSQDYVRTAFAKGLSEKSVILFHAVRNSLIPIATNIGHVIGLFIVG
                     SYVIEKLFGID"
BASE COUNT          453 a          157 c          218 g          432 t
ORIGIN      
        1 aaataaataa tattgattac tggaatactc aaaagaaata aattcctata atgtttaatt
       61 atatcataag aagattatta ttaatgatcc ccacattctt cgggacaaca ataatggtat
      121 ttactattct tcaatatgtc ccaggaggcc catttgaaag agctgtaatg caactaaagt
      181 tcgcaagtat gatgagtgga gaaagtgcca gtgcaggcgt gaaatctatt tcagacagtc
      241 agacattaac gccagaaata cttgaagatc tgagaatgca atatggattg gataaaccag
      301 ttgttattag atatttaatt tggcttggta tttggccaaa agaaataaaa aataggacag
      361 ttaatattgg ggaaacattt agggaaaata tagattcatt taaaaaagat gcatttacca
      421 gctatacatt gcaaaaatgg atcaaacccg ttaaaaataa aacaggtgaa attatagtta
      481 cggaaagtgg tgttggtatt gatataaaaa atgctgattt tgaacctaca aaagattttc
      541 aagaattacc taatgttaat tttattaaaa catggtatca ctctactaat tggaaaatag
      601 ctgaaatcaa aagtgattca gaaataagaa ttatacaaaa atcttatcaa ggtttgttta
      661 ctggatattt aggctattca aatgaaaagg ctgatgatgt tggtactcta ataaaaaata
      721 gattacatat atcattgatt tttggtttga ctagttttat tttagtttat ttaatctgta
      781 taccacttgg cattatgaaa gctataaagc atggttctaa atttgatgtt atttcaagtt
      841 cggtagtttt tatcggttat tctatcccag gatatgcact tggaacacta ttattagttt
      901 attttgcagg tgaaaatttt atggatatat ttccacttgg cggatggaga tcagaaaatt
      961 ggaataattt aacatttatg ggaaaagttt ttgatcaaat acatcatgct atattaccta
     1021 tactctgcta tatggtaggc tcatttgcaa cacttactgt actgatgaaa aattcattaa
     1081 tggaaaactt aagccaagat tatgtacgta ctgcttttgc aaaaggtctt tcagaaaaaa
     1141 gtgtaatttt atttcatgct gttagaaatt cattaatccc cattgcgaca aatatcggtc
     1201 atgttattgg tctatttatt gttggatcat atgtaattga aaaattattt ggtatcgatg
//
LOCUS       DTVV01000048            1616 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1147511, whole genome shotgun sequence.
ACCESSION   DTVV01000048 DTVV01000000
VERSION     DTVV01000048.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1147511"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="EYO86_00645"
     CDS             <1..1222
                     /locus_tag="EYO86_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIC41576.1"
                     /translation="ELHRQQNQIELIASENIASLAVLNAQGSVMTNKYAEGYPGRRYY
                     GGCEFVDQAEEIALDRVKKLFNCKYANLQSHSGAQANQAVFLALLQPHDTFLGMSLAS
                     GGHLTHGAKPNISGKWFNAIQYGVKKEGNDKDLIDYDEVEKLAKEHKPKIIIAGASAY
                     PRTIDWKRFRQISDAVGAYFMVDMAHYSGLIAGKQYPNPIEYADVVTSTTHKTLRGAR
                     SAMILTNSEEIIKKINSAVFPGLQGGPLMHVMAAKAVTFGEALKPEFEEYAKNVISNA
                     KILADVFVQKGFRVVTGGTDTHIVWLDLSPKNLTGDKAEKLLEDVGIACNKNAIPYDP
                     NPPKITSGLRIGTAAATARGFNNEDFKTVGNMIVSIIDSLLLEDSKIKEISTQTKKEV
                     VDLCSRYPIYAKAF"
     gene            1222..>1616
                     /gene="nrdR"
                     /locus_tag="EYO86_00650"
     CDS             1222..>1616
                     /gene="nrdR"
                     /locus_tag="EYO86_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HIC41577.1"
                     /translation="MRCPFCSNQDSQVKDSRPTEDNTAIRRRRICDQCGSRFTTFERI
                     QLRDLIVLKTNGKKEVFDRDKMFRSLSLALRKRNIDQEKIEKIVNAIVRKLENFGDTE
                     VKSTLIGEYIMEALSHLDQIVYVRFASVY"
BASE COUNT          574 a          246 c          316 g          480 t
ORIGIN      
        1 agaacttcat cgtcaacaaa atcaaattga gttaattgca tctgaaaata ttgcttcatt
       61 agcggttctt aatgcccaag gatctgtaat gactaataag tacgctgaag gctatcccgg
      121 aaggagatat tacggaggat gtgagtttgt tgatcaagct gaggaaatcg ctctagatag
      181 ggtaaaaaaa ttattcaatt gtaagtacgc caaccttcag tcgcatagtg gagctcaagc
      241 aaaccaagcg gtatttttag ccttattgca accccatgat accttcctag gcatgtctct
      301 tgcttcaggc ggccacctaa cacatggagc caaaccaaat atttcgggta aatggtttaa
      361 tgcaattcaa tatggcgtca aaaaagaagg taatgacaaa gatttaattg attatgatga
      421 ggttgaaaaa cttgcaaaag aacataaacc taaaattatt attgcgggcg cttctgctta
      481 tccaagaaca attgattgga agcgatttag acaaatttca gatgcagttg gtgcatattt
      541 tatggttgat atggcccact attctggttt gatagcaggt aagcaatatc caaatcctat
      601 tgaatacgca gatgtagtaa cttcaacaac tcacaaaaca ttgagaggtg caagaagtgc
      661 tatgatacta acaaacagtg aagaaattat taaaaaaata aactccgctg tttttccagg
      721 attacaagga ggtcctttaa tgcatgtaat ggctgctaaa gcagttacat ttggcgaagc
      781 attgaaacca gaatttgaag agtatgctaa aaacgtaata tcaaatgcaa aaattttagc
      841 cgatgttttt gtgcaaaaag gttttagggt agtaacagga ggtactgata cccatatagt
      901 ctggttagat ttatcgccga aaaatttaac tggggataaa gctgaaaaat tgttagaaga
      961 tgttggtatt gcttgcaaca aaaatgccat accttatgac cccaatccac caaaaattac
     1021 aagcggatta agaattggaa cagcagcagc tactgcaaga ggctttaata atgaagactt
     1081 taaaactgtt ggcaatatga ttgttagtat tatagatagt ttattactag aagattcaaa
     1141 aataaaagag atttctactc aaacaaaaaa agaagtggtt gatttgtgtt ctcgatatcc
     1201 aatttatgct aaggcttttt aatgcgctgt cctttttgta gcaaccaaga ttcccaagtt
     1261 aaggactcac gccctacgga agataataca gccattagaa gaagaagaat atgtgatcaa
     1321 tgtggatcaa ggttcacaac atttgagaga attcaattac gtgatttaat tgttttaaaa
     1381 actaatggta aaaaagaagt ttttgatagg gataaaatgt ttcgatcttt atctttggct
     1441 ttgcgtaaaa gaaatattga tcaagaaaaa attgaaaaaa ttgttaatgc catcgttcgc
     1501 aaattagaaa attttggtga tactgaagtg aaaagtactc ttataggtga atacattatg
     1561 gaagcattat ctcatcttga tcaaatagtt tatgttcgtt ttgcttcagt ttataa
//
LOCUS       DTVV01000049            1094 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1152693, whole genome shotgun sequence.
ACCESSION   DTVV01000049 DTVV01000000
VERSION     DTVV01000049.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1152693"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="EYO86_00655"
     CDS             complement(<1..392)
                     /locus_tag="EYO86_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIC41578.1"
                     /translation="MHNISKNFMLRAIELSMISLKEDGGPFGCVIVKNNEIISEGFNE
                     VTNKNDPTAHAEIVAIRNACNKLNTFNLKGTQMFTSCEPCPMCLSAIYWSHIDKIYYG
                     NNRKDAAKIGFDDSYIYDELSLDLNKRK"
     gene            complement(385..>1094)
                     /gene="xdhC"
                     /locus_tag="EYO86_00660"
     CDS             complement(385..>1094)
                     /gene="xdhC"
                     /locus_tag="EYO86_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HIC41579.1"
                     /translation="GQLEYTIIEKAKKNLNEKMILNTQIINIPLGPSIGQCCGGYAQV
                     EITKHANGIQSLINEKILNFKLDNLFIFGAGHIGQELSSRSTDLDFNVHLIDSRNDYL
                     KMQEEEKITLIYANSPWILIKNLPKESYYIILTHSHDYDFKIINEILNFNSFKFLGLI
                     GSKTKMKRFSNRLIKLGHNQTKISQIECPVGIKKITSKKPGEIAISIIGRLLEHRSSN
                     TSSLHQENKNNLKKINA"
BASE COUNT          325 a          172 c          157 g          440 t
ORIGIN      
        1 atttttcttt tgttgagatc aagtgatagt tcatcataaa tataactatc atcaaagcca
       61 attttggctg catcctttcg attgttacca tagtatattt tatcaatatg tgaccaataa
      121 attgcactta ggcacatggg gcagggctcg caactagtaa acatttgagt gcccttaagg
      181 ttgaaagtat tgagtttgtt gcacgcattt ctaatagcta caatttctgc atgcgccgta
      241 ggatcattct tattagttac ttcattaaat ccttcggaaa ttatttcatt attttttaca
      301 attacgcatc caaaaggccc cccgtcttct tttaaactta tcatagacaa ttctatagcc
      361 ctcaacataa aattttttga tatattatgc atttatcttt ttaagattgt ttttattttc
      421 ctgatgtaaa gaggaagtat tagaagatct atgttccagt agtctcccta tgattgatat
      481 agctatttca ccaggttttt tgctagtgat tttctttatt cctacggggc attctatttg
      541 actgattttt gtttgattat gtccaagttt aattaaccga ttactgaatc tcttcatttt
      601 ggttttagaa ccaatcaaac ccaagaattt aaaagaatta aaatttaaaa tttcgttaat
      661 tattttaaaa tcataatcat ggctatgtgt taggattata tagtaagatt ctttgggtaa
      721 atttttgatg agtatccacg gagaattagc ataaatcagg gtaatctttt cttcttcttg
      781 catttttaag taatcatttc ttgaatctat taaatgtacg ttaaaatcta aatctgttga
      841 tctagatgac aattcttgac ctatgtgtcc cgcaccaaaa ataaataaat tatctaattt
      901 aaagtttaag attttttcat ttattaaact ttgaatgcca ttagcatgtt tcgtgatttc
      961 aacctgagca tatcctccac agcattgccc aattgatggg cctaatggaa tatttattat
     1021 ttgagtattt aatatcattt tttcatttaa attttttttt gctttttcaa tgatagtata
     1081 ttcaagttgg ccgc
//
LOCUS       DTVV01000050            1047 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1153685, whole genome shotgun sequence.
ACCESSION   DTVV01000050 DTVV01000000
VERSION     DTVV01000050.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1153685"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(17..991)
                     /locus_tag="EYO86_00665"
     CDS             complement(17..991)
                     /locus_tag="EYO86_00665"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIC41580.1"
                     /translation="MIKLIVFLPLLGFLYCAFVGSYFHDRVSQLVTTFLLTISTFLSW
                     IIFLQYLEGANTTIIHILNWITSGTFSADWSLRVDALTATMMVVVTTVSACVHLYSVG
                     YMNEDKSINRFMGYLSLFTFFMLMLVSSNNLLQMFFGWEGVGLSSYLLIGFWHHKDSA
                     NKAAIKAFILNRVGDFGFVLGIVGVFFIFGSINFDIIFTDAINYQNHAFNFLGLSLST
                     IDTLCFLLFIGAMGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGIFMVARMSPL
                     FQYAEFTNLFIIFIGASTAIFAASIALTVTNLWLYQVLVALYLLKTPT"
BASE COUNT          405 a          196 c          156 g          290 t
ORIGIN      
        1 atacggtcaa gttttcctat gtgggcgttt tcagcaggta caaagctacc aatacctgat
       61 acaaccatag gttcgtgaca gttaaagcta tacttgctgc aaaaattgca gttgaagctc
      121 caataaaaat tataaataaa tttgtaaatt ccgcatactg aaaaagtgga ctcatacgag
      181 ccaccataaa gataccagca gtaaccatag tagcagcatg tattaatgcg gatactgggg
      241 taggcccttc catagcatct ggcaaccaag tatgtagtcc taattgagcg gacttgccca
      301 tagctccaat aaataaaaga aaacacaacg tatctattgt tgataaactt aatcctaaaa
      361 aattgaatgc gtgattttgg tagttaatcg catctgtaaa gataatatcg aaatttattg
      421 atccaaaaat aaaaaatacc ccaactattc caaggacaaa accaaaatct cctactcggt
      481 taagaataaa tgccttgatt gcagctttat tagcactatc tttatggtgc caaaaaccaa
      541 ttaataaata agaagataaa ccaacgcctt cccaaccaaa aaacatttga agcaagttat
      601 tactcgatac taacatcaac ataaagaaag taaaaaggct taaataaccc ataaatctgt
      661 taattgactt atcttcattc atatatccta ctgaataaag atgaacacaa gctgaaactg
      721 ttgtcactac aaccatcata gttgcagtta atgcatcgac tctcagactc caatctgcag
      781 aaaaagtgcc cgatgtaatc cagttcagga tatgtataat tgttgtattt gcaccttcta
      841 aatattgtaa aaaaattatc caagaaagaa aagtagatat agtcagtaag aacgtagtaa
      901 caagttgtga tacacgatca tgaaaatatg atccgacaaa tgcacaatat aaaaaaccca
      961 ataatggcaa aaaaacaatt aattttatca agttatccct ttagtttgcg tattttttct
     1021 acttcaattg agccaagatt tctaaaa
//
LOCUS       DTVV01000051            1186 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1155818, whole genome shotgun sequence.
ACCESSION   DTVV01000051 DTVV01000000
VERSION     DTVV01000051.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1155818"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1186
                     /locus_tag="EYO86_00670"
     CDS             <1..>1186
                     /locus_tag="EYO86_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIC41581.1"
                     /translation="KGIKEAYQTGRGACVIRSKISIENFKKDREAIIINELPYQVNKS
                     RLIEKIAETVHTNIIDGISDLRDESDRNGVRVVIELKRDVDSNVILNQLYKHTLLQTT
                     FNSNMLALNGGRPEQMNLKDMISSFIDFREEVVTKRIIFNLNKSRNKAHVLIGLVVAN
                     NNIDPIIKLIKSSKDSREAKEKLMKSKWKLSDSDINFITLIGDPTTKFQKKTYSLTET
                     QAKAILELRLHRLTSLEREDIKKDLENIVLEIKNYLEILNSRSKLLNVIKDELNEIKK
                     EFSTPRRSAIIDREYEQIDDISYIQQEDIIITISNNGYIKRSLLDNYRAQNRGGKGKT
                     GMSTREEDFVKEIHLATTHTKLLVFSSFGKVYSLKSYDIPEASLKARGKPIVNLLPFS
                     EGE"
BASE COUNT          504 a          147 c          187 g          348 t
ORIGIN      
        1 aaaggaataa aagaagctta tcagactgga agaggagcgt gcgtaataag gtccaaaatt
       61 tcaatagaaa attttaagaa agatagagaa gctatcatta taaatgaact gccttaccaa
      121 gttaataagt caagacttat agaaaaaata gcagaaacag ttcacactaa tataattgat
      181 ggtatttctg atttacgaga tgaatctgat agaaatgggg ttagggtagt aattgaattg
      241 aagagagatg ttgactcaaa tgtaattctc aatcaattat acaaacacac tcttcttcaa
      301 acaactttta attctaatat gctagcgctc aatgggggaa ggccagagca aatgaattta
      361 aaagatatga tttcttcctt tatagacttt cgagaagaag ttgtaacaaa aagaataatt
      421 tttaatttaa ataaatctcg taacaaagca catgttttaa taggattagt tgttgcaaat
      481 aacaacattg acccaataat caaattaatt aaatcttcta aagattctag agaagcgaaa
      541 gaaaaattaa tgaaatcaaa atggaaactt tctgattccg acattaactt tataactctt
      601 attggagatc caacaactaa atttcagaaa aaaacatatt ctttaactga aacccaagcc
      661 aaagcgatct tggagttaag actccataga ttaacaagtt tagaaagaga ggatataaaa
      721 aaagatttag aaaatattgt tttagaaata aaaaattatt tagaaatatt aaactctcgt
      781 tctaaattat taaatgtaat taaagatgaa ttaaatgaaa ttaaaaaaga attttcaact
      841 ccgagaagaa gtgcaataat tgatagagag tatgaacaaa tagatgatat tagttatatt
      901 caacaagaag atataatcat tacaatttca aataacggtt atattaaacg ttcactttta
      961 gataattacc gcgctcaaaa tagaggtgga aaaggaaaaa caggcatgtc tacacgggag
     1021 gaagattttg taaaagagat acatttagca acaactcata caaaattatt agtattttct
     1081 tcatttggaa aagtatattc tttaaaatca tatgacattc ctgaagcaag tttaaaagct
     1141 agaggaaaac cgatagttaa tcttttacca ttttcagaag gtgaaa
//
LOCUS       DTVV01000052            1170 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1162901, whole genome shotgun sequence.
ACCESSION   DTVV01000052 DTVV01000000
VERSION     DTVV01000052.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1162901"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="EYO86_00675"
     CDS             complement(<1..844)
                     /locus_tag="EYO86_00675"
                     /inference="COORDINATES: protein motif:HMM:PF04413.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41582.1"
                     /translation="MIHIYKYIGIVLIPLIKLNILIRIKQSKEVANRHKERFGITSHS
                     KPTKNLIWIHAASVGEFKSADVLINSLHKKYIVLITTTTVSAAEYAMTHYKDKIIHQY
                     APLDVDLWIKRFLQYWNPSLVIWIESDIWPITMYVLKKFKIKAILVNVRMSPQSFNRW
                     KKISFFYKQTLECFSDIFAQSQIDQKRIATLIKREVKFIGNLKLASLKKTLLENQNPN
                     FILNKKPLNLMIVSSHEGEEDKLLPMIKKLIAKNSNMRIIIAPRHPKRSTKIKSLCNS
                     LQIPS"
BASE COUNT          358 a          172 c          184 g          456 t
ORIGIN      
        1 gtgatggaat ttgaagggag ttgcacaaag atttaatttt agtcgatctt ttaggatgac
       61 gtggagctat aataattcgc atatttgaat ttttagctat caattttttt atcataggta
      121 gcaatttgtc ttcttcccct tcatgtgaac ttactatcat gaggtttaac ggttttttgt
      181 taagtataaa atttggattt tgattttcta aaagtgtttt ttttaaagat gctaatttta
      241 aattaccaat aaattttacc tctcttttta tgagggttgc aattcgtttt tgatctattt
      301 gactttgtgc gaatatgtct gaaaaacatt ccaatgtttg tttataaaag aatgaaattt
      361 ttttccatct attaaatgac tgaggagaca ttctgacgtt aacgagtatt gcttttatct
      421 taaatttttt taatacatac attgttattg gccatatgtc agattcaatc caaatgacca
      481 aagatggatt ccaatattgc aaaaatcttt ttatccataa atcaacatca agtggagcat
      541 attgatgaat aattttatcc ttataatgtg tcattgcata ctccgcagca ctgacagtgg
      601 tagtagttat tagtacaata tattttttgt gaagactatt aattaaaaca tcagcagatt
      661 taaattcccc aacacttgct gcatgtatcc atattaaatt ttttgtaggt ttagaatgcg
      721 aagttatgcc aaatctctct ttgtgtcgat tggcaacctc tttactttgc ttaattctga
      781 ttaaaatatt tagcttaatc agagggatca atacaatccc tatgtatttg tatatatgaa
      841 tcattgacag tttgcctcag ccttttgaac acatttgtta atcttgtctt ctagtaattt
      901 ttgataacta taaacttgat catcttttaa atcagcaggt acttctaggg gttcactcca
      961 tacaaaggag catttagaaa aagggagaga gattagaaat gaatcccatg acttgagttt
     1021 aaaattccta gaagaagcaa atcctaaggc aataataggt gttttggaaa ctttagcaat
     1081 tttgattatt ccttcagaca ctttttcttt tggacctcgc ggaccatccg gagttattcc
     1141 tatgtatttt ctgtttttta ataatttaaa
//
LOCUS       DTVV01000053            1951 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1163307, whole genome shotgun sequence.
ACCESSION   DTVV01000053 DTVV01000000
VERSION     DTVV01000053.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1163307"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /gene="glpK"
                     /locus_tag="EYO86_00680"
     CDS             complement(<1..1356)
                     /gene="glpK"
                     /locus_tag="EYO86_00680"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIC41583.1"
                     /translation="MKNKKKFIVAIDQGTTSSRAVLFDTAGRFIFKSQLEFKQHFPKN
                     GWVEHNPEEIWNKTKKVLIDVINKGKKLKGDILTIGITNQRETTILWDRITGKPVYNA
                     IVWQDRRTADFCKKYRSNKREQIINKKTGLLIDPYFSGTKIKWIIENVPKAKKLLKKK
                     QLLFGTIDTFLLWRLTKGAIHATDATNASRTMLYNINTNNWDNQILQSLKIPSHILPI
                     VKNSSDNFGVTHKSITGQSYPITAVVGDQQAATIGQCCFKKGSVKSTYGTGAFVLMNT
                     GYKKIYSKNRLLTTVCYRLKGKSTYALEGSIFIAGAGVQWARDKMKLIKKASDTEKFA
                     KSLKSNSGVYLVPAFVGLGAPYWDSNARGVLSGLTRNTGVKEIIRAIIESTAYQSYDL
                     FNAMKKDGLKPKVIKIDGGMVRNNWFSQFLSSVLNITVYRSQNEETTALGVAFMAGLQ
                     IG"
     gene            complement(1362..>1951)
                     /locus_tag="EYO86_00685"
     CDS             complement(1362..>1951)
                     /locus_tag="EYO86_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIC41584.1"
                     /translation="LANKVHTSVVFANTDIKIARQISQLVSTEYYHVFASDDVIGVET
                     SAAIKNIFSMAVGASQGLCHPNSSGVIKEKNYLNTAAALIQESIYEMIIFTERLKGKK
                     ETVLGLAGIGDLYVSADGGRNSKMGEYLGKGMNFKEAKKTKMPNDTIEGADLAQEIGS
                     KVKSDFDNKELPLMTCMINTICDETPLKVDWKNFK"
BASE COUNT          588 a          369 c          249 g          745 t
ORIGIN      
        1 acctatttgt aatcccgcca taaaagctac acccaatgct gtagtttctt cattttgaga
       61 tcgataaact gttatattta atacgcttga taaaaattga gaaaaccaat tatttcttac
      121 cattcctcca tcaatcttaa tgactttagg ttttaatcca tctttcttca tagcattgaa
      181 taaatcataa ctttgataag cagttgactc aataatagct ctaataattt cctttactcc
      241 agtatttctt gttaaaccag acaaaactcc tcttgcatta gaatcccaat aaggagcacc
      301 taagccaaca aaagctggaa ctaaataaac cccactattg cttttaagcg attttgcaaa
      361 tttttctgta tcgctagcct ttttaattaa tttcatttta tctcttgccc attgaacacc
      421 tgctccagca ataaatattg aaccttctaa agcataagta gatttacctt tcaatctata
      481 gcaaaccgtt gttaataatc tattttttga atatattttt ttgtagcctg tattcatcaa
      541 tacaaatgct cccgttccat aagtactttt tactgaccct tttttaaaac aacattgacc
      601 gatggtagcg gcttgctggt ctcctactac agcagtaata ggataggatt gtcctgtgat
      661 agatttatga gtaactccaa aattatcaga tgaattttta actatgggaa gtatatgaga
      721 tggaattttg agactttgaa gtatttgatt atcccaatta ttagtattaa tattatacaa
      781 cattgtccta gaagcatttg ttgcatcagt agcatgaata gcacctttag ttagtctcca
      841 taacaaaaat gtgtctattg ttccaaagag aagttgtttt ttttttaata gttttttagc
      901 tttaggtaca ttttctatta tccatttaat ttttgttcct gaaaagtaag gatcaatgag
      961 aaggcctgtt tttttattaa ttatctgctc tcttttattt gatctatatt tcttacaaaa
     1021 atcagctgtt cttctatctt gccaaacaat ggcgttatag acgggctttc ctgtaattcg
     1081 atcccataaa atagtagttt ctctttgatt ggttatccct atagttaaaa tatctccttt
     1141 aagttttttg cctttattaa ttacatcaat caataccttt tttgttttat tccatatttc
     1201 ttcaggatta tgctcaaccc atccattttt tgggaagtgt tgtttaaatt ctaattgaga
     1261 tttaaatata aatcttccag cagtatcaaa taaaacggct ctacttgatg ttgttccttg
     1321 atctatagct acaataaatt tttttttatt tttcaacgga cttacttaaa atttttccaa
     1381 tcaaccttta gaggtgtttc atcacaaatc gtattaatca tgcatgtcat taaaggaagt
     1441 tctttattat caaaatcact tttcaccttt gatccaattt cttgagcaag atccgctcct
     1501 tcaatagtat cgttaggcat ttttgttttt tttgcttctt taaaattcat accttttcct
     1561 agatattcac ccatcttact atttcttcct ccatctgcac tcacatataa atcaccaatc
     1621 cccgcaagcc ccaaaactgt ttcttttttt ccttttagtc tttctgtaaa aatgatcatc
     1681 tcatatatag attcttgaat gagagcagct gctgtattca aataattttt ttcttttatc
     1741 acccctgatg aattcggatg gcacaaacct tgtgaagcac ccacagccat tgaaaagata
     1801 tttttaattg cagccgacgt ttctacacct ataacatcat cagacgcaaa aacatgatag
     1861 tattcagttg aaactagttg agaaatttgt ctagcaattt taatatccgt attggcaaaa
     1921 acaacggacg tatgaacttt attagctaaa c
//
LOCUS       DTVV01000054            2219 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1166914, whole genome shotgun sequence.
ACCESSION   DTVV01000054 DTVV01000000
VERSION     DTVV01000054.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1166914"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="EYO86_00690"
     CDS             complement(<1..189)
                     /locus_tag="EYO86_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HIC41585.1"
                     /translation="MLIEQDTVNLYQDQGVVLVKKIISSNWIKKLKAGIKKNFENPSQ
                     YKCVYEKINDKELFYDDYC"
     gene            complement(190..951)
                     /locus_tag="EYO86_00695"
     CDS             complement(190..951)
                     /locus_tag="EYO86_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004775873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC41586.1"
                     /translation="MNYYGVKAIYLHEMDRMRRTLFQSLVSPLISTSLYFIVFGSAIG
                     SRIKEVDGVAYGSFIVPGMIMLTLLTQSISNASFGIYFPKFTGTIYELLAAPISSFEI
                     ILGFVGAAATKSLIIGCVIIATAGLFVDLNIAHPLVMMFFLIFTAVTFSLFGFIIGFW
                     ADNFEKLQLIPILIITPLVFLGGSFYSINMLPEFWQKVSLFNPVLYLVSGFRWSFYEI
                     ADVSIFTSLATMFFILFSCITIISMSFAKGYKIKN"
     gene            complement(932..1876)
                     /locus_tag="EYO86_00700"
     CDS             complement(932..1876)
                     /locus_tag="EYO86_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC41587.1"
                     /translation="MKNFISIVNLSKVYANNFKALDEINLEIEKGEIFALLGPNGAGK
                     STLINIICGIVTSSAGKILVNNFDIKKEYRKARSLIGIVPQELTLEAFETVWDNVCYS
                     RGLYGKSLNSNYIESLLKELSLWEKRKSKLRELSGGMKRRVLIAKALSHEPTVLFLDE
                     PSASVDVELRKDMWGIVKRLKQKGVTIILTTHYIEEAEEIADRVGIINHGKIIVVDQK
                     DELIKRLGEKKLILELQETINNIPTILQEFNLELSEDKKRITYTYDTKNKRTGITTLL
                     NLMRDEGINLKDMNTEKSSLESIFINLVKGSNNELLRS"
     gene            complement(1894..2130)
                     /locus_tag="EYO86_00705"
     CDS             complement(1894..2130)
                     /locus_tag="EYO86_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41588.1"
                     /translation="MSIYVDSLLIDKGNTNKVLENPINSAIWVINQLALKGEPLLKGQ
                     FISTGTCTKAIQIKPGNTIKADFGSIGSVKLNYV"
BASE COUNT          755 a          381 c          278 g          805 t
ORIGIN      
        1 acagtagtca tcataaaaaa gctccttatc atttattttt tcataaacac atttatattg
       61 gcttggattt tcaaaatttt ttttaattcc tgcttttaat ttttttatcc aatttgaaga
      121 aattattttt ttaacaagaa ctacaccctg atcttgatat aaattaacag tatcttgttc
      181 tattaacatt taattcttaa ttttatatcc cttagcaaaa ctcatggata ttattgtaat
      241 acaagaaaaa agaataaaaa acatagttgc taaacttgtg aatatactta catcggctat
      301 ttcgtaaaaa ctccatctaa aaccacttac taaatataat acaggattaa ataaagacac
      361 tttttgccaa aactccggaa gcatattgat cgaataaaaa ctccctccta gaaatactaa
      421 tggggtaatt ataagaatgg gaattaattg tagtttttca aaattatctg cccagaaacc
      481 aataataaaa ccgaataagc taaaagttac tgctgtaaaa attaaaaaaa acatcataac
      541 taaaggatgt gctatgttca aatcaacaaa caaacctgca gtagcaataa taacacaccc
      601 aatgatcaaa gattttgttg cagctgctcc aacaaagcct aatataattt caaagcttga
      661 tattggtgcg gctagtaatt catatattgt tccagtaaat ttagggaaat agattccaaa
      721 agaagcatta ctaatagatt gtgttaaaag tgtcagcatt atcatcccag gaacaataaa
      781 tgatccgtac gcaaccccgt caacctcttt tattcgtgag ccgatagctg atccaaaaac
      841 aataaaatat aatgatgtgg aaataagagg tgacactaaa ctttgaaata aagttctgcg
      901 catcctgtcc atttcatgga gataaatagc tttaactccg taataattca ttattagacc
      961 ctttcactaa atttataaat atactttcaa gtgagctttt ttcagtattc atatctttta
     1021 gattgatacc ttcatctcgc attaaattta aaagtgtagt aattcctgtt cttttatttt
     1081 tagtatcata tgtgtatgta attcgcttct tatcttcact taattcaaga ttaaattctt
     1141 gtaaaattgt aggaatatta tttattgttt cttgtagttc gagtattaat tttttttcac
     1201 ctagtctttt gattaattca tctttttgat caacaacgat tatttttcca tgattaataa
     1261 taccaactct atctgcaatt tcttcagctt cttcaatata atgagtagtt aaaataatag
     1321 taacgccttt ttgttttaat cttttaacaa ttccccacat atcttttctt aattccacat
     1381 ctacacttgc gcttggttca tctaaaaaaa gtactgtagg ttcgtgtgaa agtgcttttg
     1441 caataagaac tcttctcttc attccaccag ataattctct aagtttactt ttgcgttttt
     1501 cccatagaga aagctcctta agtaatgact caatataatt ggagtttaat gatttcccat
     1561 acaaccctct tgaataacag acattatccc atactgtttc aaaagcttct aaagttaatt
     1621 cctggggaac tataccaatt aatgatctag cttttcgata ttcttttttt atatcaaaat
     1681 tatttactaa tatcttgcca gcagaagatg taactatccc acaaataata ttgattaatg
     1741 ttgatttacc tgctccattt ggtcctaaaa gtgcaaatat ttcacctttt tcaatttcta
     1801 aattaatttc atctaaggct ttaaaattat ttgcataaac tttgcttaaa tttacaatag
     1861 aaataaagtt tttcatattt ttataaagat aatttaaaca taatttaatt taacagatcc
     1921 aatacttcca aaatcggctt taatagtatt tccaggctta atttgtattg ccttggtaca
     1981 tgttcctgtt gaaataaatt gaccttttaa taaaggctcg cctttaaggg ctaattgatt
     2041 aataacccat atagctgaat ttatggggtt ttcaagaact ttgttggtgt tccctttatc
     2101 aattaataaa gaatcaacat aaatactcac aacatcttac tatggacatt gcacccacct
     2161 gcctgcggca gtgagcgtat ggaacgggaa ctgtcctggt ctcggcatca ttctgtgta
//
LOCUS       DTVV01000055            1412 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1167114, whole genome shotgun sequence.
ACCESSION   DTVV01000055 DTVV01000000
VERSION     DTVV01000055.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1167114"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="EYO86_00710"
     CDS             complement(<1..277)
                     /locus_tag="EYO86_00710"
                     /inference="COORDINATES: protein motif:HMM:PF05013.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIC41589.1"
                     /translation="MKFDHSYKPYSIYNHNEHSKIIILCDHASKTIPKKYKNLGLSTK
                     NVNKHIGWDIGALKLAKKISQKTKSTFIYSGYSRLLIDCNRALQTKGA"
     gene            complement(317..>1412)
                     /locus_tag="EYO86_00715"
     CDS             complement(317..>1412)
                     /locus_tag="EYO86_00715"
                     /inference="COORDINATES: protein motif:HMM:PF00120.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41590.1"
                     /translation="EFESYSILHHRPGVARFNSIMLDDKGQVWEGCPRSQLKKITEEF
                     HKLKITIKLSFEPEFSIYKKENENLIPISFDGMFTISGMDKINQIWNDLSQTISKANI
                     KIEQVGKEYGPGQYEATWTYDDVIKSIDNYINFRDFIKSIIRDHGYLATFMPKPFDHL
                     PGNGLHLHISLWDQNNKEISCSDNDEFPLSNIGANFVAGLLKNAHSLTATGCATVNSY
                     KRILPGSWSPAHICWGIENRSTLIRIPGKGNRRHIELRQGDNSNNPYIYLISVLASGL
                     EGIKNKLTLEDPIAEDVGQFSDKNNDNKLKVLPRTFNESLTYLKENEVIEKALGSIIF
                     EEFIKIKETEVRQYETTVHSWERNKYIDIF"
BASE COUNT          448 a          228 c          191 g          545 t
ORIGIN      
        1 aagctccttt tgtttgaaga gctctattac aatcaattaa aagtctagaa taacctgaat
       61 agataaaagt agattttgtt ttttgtgaaa ttttttttgc aagtttcaat gctccaatat
      121 cccaccctat atgtttgtta acattttttg ttgaaagacc taagttttta tattttttgg
      181 gtatagtttt cgaagcatga tcacaaagta ttattatttt actatgctca ttgtgattat
      241 atattgaata cggtttataa gaatgatcaa acttcattaa tttttttaat agttaaattt
      301 taataaaaaa aacaaactag aaaatatcaa tatatttatt tctttcccag gaatgaacag
      361 tcgtttcata ttgtcttacc tctgtttctt ttattttaat aaattcttca aagattatag
      421 aaccaagtgc tttttcgata acttcatttt cttttaggta agttaatgac tcattaaatg
      481 ttcgaggaag aacttttaat ttgttatcat tatttttatc tgaaaactga ccaacatctt
      541 cagcaatcgg atcttccaaa gttaacttat tttttatacc ttctaaacca gaagcaagaa
      601 ctgaaattag ataaatataa ggattatttg aattatcgcc ttgtcttaac tcaatatgtc
      661 ttctgttacc tttcccagga attcttataa gagtagatct attctctata ccccaacaaa
      721 tatgtgcagg tgaccatgaa cctggtaaga ttcttttata tgaattaaca gttgcacacc
      781 cagttgcagt gagagaatga gcatttttaa gtaagccagc tacaaaattt gctcctatat
      841 ttgataaagg aaattcatca ttatccgaac agctaatttc tttgttattt tgatcccata
      901 aacttatatg aagatgtaaa ccgtttcctg gtaaatgatc aaaaggtttt ggcataaaag
      961 ttgctaaata accgtgatcc ctaatgatgc ttttaatgaa atccctaaaa tttatataat
     1021 tatctataga tttaatgaca tcatcatatg tccatgtcgc ttcgtactga ccgggaccat
     1081 attcttttcc tacttgctcg attttaatat ttgctttgga gatggtttga gaaaggtcat
     1141 tccatatctg atttatttta tccattcctg aaatcgtaaa catgccatca aaactaattg
     1201 gaattaaatt ctcattttct ttcttataaa ttgagaactc tggttcaaag ctcagtttaa
     1261 ttgtaatttt taacttgtgg aattcttctg ttattttttt taattggctt cttgggcaac
     1321 cttcccatac ttggccttta tcatccaaca taatggagtt aaatctcgca actccaggtc
     1381 gatgatgtaa aatgctataa ctttcaaact ca
//
LOCUS       DTVV01000056            1115 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1168029, whole genome shotgun sequence.
ACCESSION   DTVV01000056 DTVV01000000
VERSION     DTVV01000056.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1168029"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            26..553
                     /locus_tag="EYO86_00720"
     CDS             26..553
                     /locus_tag="EYO86_00720"
                     /inference="COORDINATES: protein motif:HMM:PF08264.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41591.1"
                     /translation="MLHEKIKIKISKTHEINSDVISKFTNQLIDKINNNLERFNYNVI
                     IASMYETYNFLNQKINEPINSQDLLGNYRKILSIFSPVLPHLTSECLEDLNLDPFQKW
                     PSVDKNLLANEMIEYVIQINGKKRGVIKAAKDITDNDLMTQIQKDVKNNKIIKNRNIN
                     KIFFVKNRLINILFK"
     gene            550..1011
                     /locus_tag="EYO86_00725"
     CDS             550..1011
                     /locus_tag="EYO86_00725"
                     /inference="COORDINATES: protein motif:HMM:PF04390.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41592.1"
                     /translation="MMKIYKLNYLILSFLFIVSCGYQPLYSNKETNFYIYKINSFGDE
                     KINKALINKLELYKDKNSNKKIELEINSKINKTTTSKDTKGNPKTFRIEIISQIKVIE
                     GEKINLDKIFSKSSNYNNSSKKFELKQYEENLKINLIDKISEDIVGYLQTL"
BASE COUNT          531 a          106 c          121 g          357 t
ORIGIN      
        1 atataaattt atccaaaaac tttggatgct gcatgaaaaa ataaaaataa aaataagtaa
       61 aacacatgaa ataaattcag atgtaatctc aaaatttact aaccaattaa ttgataaaat
      121 aaataataat ttagaaagat ttaattacaa tgttataata gcaagtatgt atgagacata
      181 taatttttta aatcaaaaaa ttaatgaacc aattaattct caagatttac ttggaaatta
      241 tagaaaaata ttatcaatat tttcaccagt attacctcat ttaacatctg aatgtttgga
      301 ggatcttaat ttagatcctt ttcaaaaatg gccttccgtg gataaaaatc tactagcaaa
      361 tgaaatgata gagtatgtta tacagatcaa tggaaaaaaa agaggagtaa taaaagctgc
      421 aaaagatatt acggataatg atctaatgac tcaaattcaa aaagatgtaa aaaataataa
      481 gataattaaa aacagaaata ttaataaaat tttttttgtt aaaaatagat taataaatat
      541 tttattcaaa tgatgaagat atataaatta aattacttga ttttatcttt tttatttatt
      601 gttagttgtg gatatcagcc tttatattca aataaagaaa caaattttta tatttacaaa
      661 ataaattcat ttggtgatga aaaaatcaac aaagctttaa taaataaatt agaattatat
      721 aaagataaga atagtaataa aaaaattgaa ttagaaatta actccaaaat aaataaaact
      781 acaacatcca aagatacaaa aggaaatcca aaaacattta ggatcgaaat catttctcaa
      841 ataaaagtta ttgaaggaga aaaaataaat ttagataaaa ttttttcaaa atcttccaat
      901 tataataatt cttcgaaaaa atttgaacta aaacaatatg aagaaaatct aaaaataaat
      961 ttaattgata aaatttccga agatatagtt ggttatttgc aaacactcta aattcacatg
     1021 attattaaaa ctttcgagtt gcaaaaatta aaggcaacaa aagcaaaaaa atttttattt
     1081 tatggagaaa atgaaggtta caaagatcaa ataat
//
LOCUS       DTVV01000057            1053 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1168382, whole genome shotgun sequence.
ACCESSION   DTVV01000057 DTVV01000000
VERSION     DTVV01000057.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1168382"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
BASE COUNT          401 a          133 c          137 g          382 t
ORIGIN      
        1 cttttgcaaa aatatacaat ttagatcaag caaaagaatt gttaaattta atgaaacctg
       61 gttacgaaaa agcaatggct atgtcactta ttggaaagga acttgctgct aaaaaaaaca
      121 tctcagtaat ggaatatttt ttaaatgaag ctttaaaaac agttcctaat ttggttggag
      181 gaaagtatgc tcatggttta ccaggtttct caacaaaagg tagggttttt atggaagttg
      241 ctacaacata tgctttagct gaaaattttg aaaaatctca taaattacta ggattaatag
      301 aaagtgatag attttataag gaaggcattt ccgaagttat tataatccaa tcgcataaag
      361 ataaagctgg agcaaaaaaa ctagcgttaa aaatgcttga gcatggaggt aaaattattg
      421 ataacaaatt tattggaaaa atagcttatg ctcaagcaat tagtggagat attgaaaatt
      481 cactaaatac tataaaaact atggaattag gctttgatta ttctcaggcc ttgataaata
      541 tcgcaaataa aatcaatctt caacaagaag atcttcaaac ttcgtatcac tgatattttt
      601 ttttgttgaa gatagtttat aagaccaatt ctcaatatta ttaacaaaaa ttttttcatt
      661 atctttaagt tttaatactg attgattttc aatatcaaaa ctaatccatt tatctttatc
      721 tttattaaaa ataattgcaa aaatatttgt gccatcaaat aattcaaaat ttatttttgt
      781 aagagattta ttgtcaataa tatctttacg tttaacaaca tcttcaaaaa ttaaattagc
      841 aaaaaaataa cctataaaat ttccaattaa atcgcttttt ggaatttgtt ctttagttag
      901 gttatcaatt aaaaagtttt catttttatc atctttagtt aaagaaaagg attcactttt
      961 atttaaatga tttattgatg ctttttttac atcttgataa tctatatcta aaatttttgt
     1021 ctctaaccaa tccatctcaa ctgatgacat atc
//
LOCUS       DTVV01000058            1981 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1171748, whole genome shotgun sequence.
ACCESSION   DTVV01000058 DTVV01000000
VERSION     DTVV01000058.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1171748"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            240..1226
                     /gene="rhaS"
                     /locus_tag="EYO86_00730"
     CDS             240..1226
                     /gene="rhaS"
                     /locus_tag="EYO86_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC41593.1"
                     /translation="MKKILLVLFSIVLFSANVSAADMRIALVVKGLGIGFFEAAAEGG
                     QEAAEDIGGVEVIYTGPATTTAEEQIEVINSLIAQKVDAIAISANDRDALVPIGKKAM
                     ERGITVISWDSGIGEEGRIMNLDPSNTALIGSSNIKWMKNSMGDEGEFAILSATSQAT
                     NQNAWIEEMKKEFAKPEYSKMKWVDTVYGDDLFDKSYQEALGLFKKYPNLKGIIAPTS
                     VGIVAAAKAVEDQGLVGKVFITGLGLPSEMQAHVKSGAAVQFGIWNPIDLGYSAVYLS
                     YQIATGKYKGEEGECIPVGRMGEICIGEGNSAAMADPFVFDISNIDKFAKIF"
     gene            1265..>1981
                     /locus_tag="EYO86_00735"
     CDS             1265..>1981
                     /locus_tag="EYO86_00735"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIC41594.1"
                     /translation="MFLIPMKLELTNISKSFPGIKALNNVNFYLKQNSVHGLIGENGA
                     GKSTLVKILCGVYQPEEGKIKINQKEVNLDNPKESLRFGISAIHQEPVMFDELTVMEN
                     IFIGNHIIKKNNFIDWNQMTKKSDEVLAALETNIKSSDKVKSLSIAQRHIVQISRSLA
                     QDADIVIMDEPTAALSQKEIQELYKIITNLQKQNKSIIFISHKLDEILEICDEFTVLR
                     DGGVVSADLISNTSKNELISN"
BASE COUNT          696 a          277 c          368 g          640 t
ORIGIN      
        1 gataatttat ttgatttatt attccaaata tttgtaactg ggcgttgaaa gttcataaaa
       61 aaacaattaa ctcatagatt aaagataaac aatatttttt ggttttattc ttattaaatt
      121 cttaataatc cttcaaacat tttcattttc tttcattatt caaagctaaa ttttgaaatt
      181 atcttgataa tatttattta aagtaaatag tgtttcgtaa atttataaat ggaggaataa
      241 tgaaaaaaat tttattagtt ttattttcta tagttttatt ttctgcaaat gtaagcgctg
      301 ctgatatgcg tattgcactt gtggttaaag gtttaggtat tggtttcttt gaagctgctg
      361 ctgaaggtgg tcaagaagct gcagaagaca ttggtggtgt agaagttatt tatactggtc
      421 cagcaacaac tactgcagaa gagcaaattg aagttattaa ttctttaatt gcgcaaaaag
      481 ttgatgcaat tgctatatcg gcaaatgatc gcgatgcact tgtgccaatt ggcaaaaaag
      541 caatggaacg tggtattact gtaatatctt gggactcagg tatcggtgaa gaaggtagaa
      601 taatgaatct tgatccttcg aacacagcat taattggatc ttcaaatata aaatggatga
      661 aaaattctat gggtgatgaa ggggaatttg caattctgag tgccacaagt caagcaacta
      721 atcaaaatgc ttggattgag gaaatgaaaa aagaatttgc taaacctgaa tactctaaaa
      781 tgaaatgggt tgataccgta tacggtgatg atttatttga taaaagttat caagaagctc
      841 ttggtctttt taaaaaatac ccaaatctta aaggtatcat tgcaccaaca tcagtcggta
      901 ttgttgctgc agcaaaagct gttgaagatc aaggtctagt tggtaaagta tttattactg
      961 gtcttggcct ccccagtgaa atgcaagcac acgttaaaag tggcgcagct gtgcagtttg
     1021 gtatctggaa cccaatcgat ttaggttact cagctgttta cctttcatat caaattgcga
     1081 caggtaaata taaaggtgaa gaaggcgaat gtattcctgt tggaagaatg ggtgaaattt
     1141 gtatcggtga aggaaactcc gcagctatgg ctgatccatt tgttttcgat atttcaaata
     1201 tcgataaatt tgctaaaata ttctaattaa attttaatac atacgaccat tttttaatgg
     1261 tcgtatgttt cttataccaa tgaaactaga acttacaaat atcagtaaat catttcctgg
     1321 aatcaaagct ctcaacaatg ttaattttta tttaaaacaa aatagtgtgc atggcctaat
     1381 aggggagaat ggtgccggca agtcaacact cgttaaaatt ttatgtggtg tgtatcaacc
     1441 agaagaagga aaaataaaaa ttaatcaaaa agaagttaat ttagataatc ccaaagagtc
     1501 cttaagattt ggcatttcag ctattcacca agagcctgta atgtttgatg agttaaccgt
     1561 tatggaaaat atttttattg gtaatcacat tatcaaaaaa aataatttta ttgattggaa
     1621 tcaaatgacg aaaaaatctg atgaggtgtt agcggcactc gaaacaaata ttaaatctag
     1681 tgataaagtt aaaagtctca gtattgctca gcgccatatt gtgcaaatat caagatcgtt
     1741 ggcgcaagat gctgacatag tgatcatgga tgagcctact gcagccctct cacaaaaaga
     1801 aatacaagaa ttatataaaa ttataaccaa cttacaaaaa cagaataaat cgattatttt
     1861 tatttctcat aaattagatg aaattctaga aatctgcgat gaatttaccg tgttgcggga
     1921 cgggggagtt gtgtcagctg acttaattag caacacatct aaaaatgaat taatttcaaa
     1981 c
//
LOCUS       DTVV01000059            3409 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1174056, whole genome shotgun sequence.
ACCESSION   DTVV01000059 DTVV01000000
VERSION     DTVV01000059.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1174056"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="EYO86_00740"
     CDS             complement(<1..697)
                     /locus_tag="EYO86_00740"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:PF00206.18"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIC41595.1"
                     /translation="MKKSPARNVHLKKGPSKLLEKINSSIEVDARLYKEDIQASIAHA
                     HMLIKTKIISAQEGKKIIVALQQIFRSIDKGKVRFEKKYEDIHMNIEALLQKKIGTVA
                     GKLHTARSRNDQVVTDFKLWIKKHSLVIDQSCQAFQKALIGVAKKNITTVMPGYTHLQ
                     IAQPVSLAHHFLAYVEMIGRDRGRLKDCIKRLNESPLGAGALAGTSFPINRHMTSKIL
                     GFNQPTKNSIDSVS"
     gene            813..3092
                     /locus_tag="EYO86_00745"
     CDS             813..3092
                     /locus_tag="EYO86_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIC41596.1"
                     /translation="MDIKKVVVIGSGTMGSGIAAQVANAGIPVFLLDLPSSEGTRNKI
                     VENAKERIANSRPPLLVEKSKIDLIHVGNIEDNFNLVGEADWVVEAVVERIDIKHSIY
                     KKIEDVRKPESVISSNTSTIPLKVLSENMSDEMKQNFCITHFFNPVRYMGLLEIVTEP
                     INDKEKINLLKSFCDEKLGKGVIICKDTPGFLGNRVGVYAMQVAMTEAFKMGLTIEEA
                     DAVFGRPMGIPKTGVFGLYDLIGIDLMSDVLKSFIKELPKDDPFHEVAQEKPLITKLI
                     SDGYTGRKGKGGFYRINKEGEKKVLESINLKTGEYSKTKKVDLETETLDFIYLINRID
                     KFGEYAWSVLSKIILYASSLIPKVTDEYNNIDEAMRLGFNWTIGPFEILDKIGIEFFA
                     ERDQNLKLNRFLNNLYLNKRIDWYSDKQLYLKHDLRTLRRRSNIYWLKADVKKNENLI
                     FNSAKIYTSETEGYNIVEFTTKANTLDSDSMYALSKATEKNLIIINDALQFSAGVNLN
                     YVMEFAKQKEWRKIQKFIFDFQQTCKKLKYSEFPVIAAPSGLAIGGGFEVVCQSDYVV
                     SHTNVVLGLVETLVGLIPAGGGCKEMLWRWMQTDESKKDLNFAPLKVFDLIGYAKTAS
                     SPNEAIPNKFLLDKDRVVINRDMLLHESEKLLLQINKDYKPPAEPTFKLSGANVRDKM
                     YEILETLYKDKKILDHGLEVGKQLAFVLSGGNTNLDKELSEDDLYALELDAFMNLIQM
                     PKTQERIKHTLETGKPLVN"
     gene            3139..>3409
                     /locus_tag="EYO86_00750"
     CDS             3139..>3409
                     /locus_tag="EYO86_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIC41597.1"
                     /translation="MNNFSAHLDKNRANYIPLTPLTFIERTKDVYPDYESVIYGNRTY
                     TWLKTYNRCTQFASALEKQGIGLGNTVSIIAANTPELLEAHYSIPM"
BASE COUNT         1180 a          498 c          630 g         1101 t
ORIGIN      
        1 cagaaacact atcaatggaa ttttttgttg gctgattaaa acctaatatt ttagatgtca
       61 tgtgccgatt aatcggaaac gaagtacccg caagagcacc cgcaccaaga ggactctcat
      121 ttagtcgttt aatacagtct tttagtcttc cccgatcacg accaatcatt tccacgtaag
      181 caaggaaatg gtgagcaagc gaaactggct gcgctatctg gagatgcgta taacctggca
      241 taacagttgt aatatttttt ttagccactc caattagtgc tttttgaaaa gcttgacaag
      301 attgatcgat gacaaggcta tgttttttta tccaaagttt aaaatcggta acaacttgat
      361 cgttgcgtga gcgtgccgta tgtaatttgc ccgcaacagt ccctattttt ttttgtaaca
      421 aagcctcaat attcatatgg atatcttcat actttttttc aaaacgcact ttacctttgt
      481 ctatactgcg aaaaatttgc tgtaatgcga caattatttt tttaccttct tgtgcagaaa
      541 taattttggt ttttattaac atgtgtgcgt gtgcaatgga tgcctggata tcctctttat
      601 aaagacgcgc atctacttca atggaggaat tgattttctc caagagtttt gacggtcctt
      661 tttttagatg gacattgcgt gctgggcttt ttttcatttc ggtgcgccta ataagagata
      721 tttataacaa tttccacccc attacttgaa taatttaatt tcaaggagtg tgattttgat
      781 ttttttatat ataaacccca ataaataagt ttatggacat caaaaaagtc gttgttattg
      841 gatctggaac aatgggaagc ggtattgccg ctcaggtagc caatgctggc attccggtct
      901 ttcttttgga cctaccttca agtgaaggca ccagaaacaa aatagtcgaa aatgccaagg
      961 aaagaattgc aaattcacgc ccccctcttt tggtagaaaa aagcaaaata gacttaattc
     1021 atgtcggcaa cattgaagat aattttaatt tggttgggga agctgattgg gtagttgaag
     1081 ctgttgttga gagaattgat atcaaacata gcatctataa aaaaatagaa gatgtccgaa
     1141 aaccagaatc agttatttct tccaacacat ctacaatacc acttaaagtt ttgtctgaaa
     1201 atatgtctga tgaaatgaaa caaaattttt gtatcacaca tttttttaat cctgttcgtt
     1261 acatgggtct tttggaaata gtcacagagc caattaatga taaagaaaaa attaatcttc
     1321 taaaatcatt ttgcgatgaa aaacttggca agggagtaat tatttgcaaa gacacacctg
     1381 gatttttagg aaaccgtgtt ggagtttatg caatgcaagt tgctatgaca gaagcattta
     1441 aaatgggatt aacaatagaa gaggcagatg cggtttttgg tcgaccaatg ggaattccta
     1501 aaacaggagt ctttggtttg tatgatttaa taggtattga tctaatgtct gatgttctaa
     1561 aaagttttat taaagaactt ccaaaggatg atccttttca tgaagtagct caagaaaaac
     1621 cacttatcac aaaattgatt agtgatgggt atacaggaag aaaaggcaag ggcggttttt
     1681 atcgtatcaa caaagaagga gaaaaaaaag ttttagaatc tattaatcta aaaacaggag
     1741 aatattcaaa aactaaaaaa gttgatttag aaactgaaac tttagacttt atatatctga
     1801 taaatagaat tgataaattc ggagaatacg cgtggtcagt tttatctaaa attattttat
     1861 atgcttcatc tttgattcct aaagttacag atgaatacaa taatattgat gaagcaatga
     1921 gattgggatt caattggact attggccctt ttgaaatact tgataaaatt ggaatagaat
     1981 tttttgctga acgagatcaa aatctaaaac ttaatcgttt tttaaataat ttgtatttaa
     2041 ataaacgaat agattggtat tcagataaac aactatattt aaaacatgat ttaagaacac
     2101 ttagaagaag aagcaatatt tattggttga aagctgatgt caaaaaaaat gagaatttga
     2161 tatttaattc tgcaaaaatt tatacttcag aaactgaagg ttataatatt gtagaattta
     2221 ctactaaagc taacacttta gattctgatt ctatgtatgc cttatcaaaa gcgactgaaa
     2281 aaaatttaat tattataaat gatgctcttc aattttcagc tggagtaaac ttaaattatg
     2341 tcatggagtt tgcaaaacaa aaagaatgga gaaaaattca gaagtttatt tttgattttc
     2401 aacaaacttg caagaaatta aagtattctg aatttccagt tattgcagct ccatctggtt
     2461 tggctattgg aggaggattt gaagtagtat gtcaaagtga ctatgtcgtt tcacacacaa
     2521 atgttgttct tggattggta gaaactttag taggattaat tcccgcaggg ggcggatgta
     2581 aagaaatgtt atggcgatgg atgcaaactg atgaatcaaa aaaagatctc aattttgctc
     2641 cattgaaagt tttcgattta ataggatatg caaaaactgc ttcatcacca aatgaagcaa
     2701 ttccaaataa atttttatta gataaagata gagttgtaat caatagggat atgttacttc
     2761 atgagtctga aaaattatta ttacaaatta acaaagatta taagcctcca gcagaaccca
     2821 catttaaatt atctggggca aatgttcggg ataagatgta tgaaatttta gagactttat
     2881 ataaagataa aaaaatcttg gatcatggat tggaagttgg gaagcaacta gcttttgttt
     2941 taagtggggg aaatacaaat ttggataaag aattaagcga agatgatttg tatgctttgg
     3001 aattggatgc atttatgaac ttaattcaaa tgccaaaaac acaggagcga attaagcata
     3061 ctttggaaac agggaaacca ttagtaaatt aaatgttttt cttataatga taaaaataat
     3121 tttttttgga gggaaaaact gaataatttt tcagcacatc tagataaaaa tcgggcaaat
     3181 tatatacctt tgacccccct cacttttatt gaacgcacaa aagatgttta ccctgattat
     3241 gaatcggtta tttatggaaa tcgcacttat acttggttga aaacatataa taggtgcact
     3301 caatttgcca gcgccttgga gaaacaagga attggtcttg ggaacactgt atcgattata
     3361 gccgccaata cgccggagct gttggaagcc cattattcca ttccgatga
//
LOCUS       DTVV01000060            1118 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1174916, whole genome shotgun sequence.
ACCESSION   DTVV01000060 DTVV01000000
VERSION     DTVV01000060.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1174916"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="EYO86_00755"
     CDS             complement(<1..920)
                     /locus_tag="EYO86_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIC41598.1"
                     /translation="MSEVYDRWPQIARKNFSVNFSKVDIKNIDHIVFAGMGGSGTIGD
                     VFASILSKTNIHVTVIKGYLLPKTVDDSTLVVTTSISGNTLETISILKNVEKMNAKFV
                     ALSSGGIMEKFCMNKKIKYYKIEEEHSPRASFVGFLYSTLNILEQIVPVKKTDVSESI
                     ESLFNIQKSVCSKNLNETNEALQIAEWIKEIPMVYYPSGLQSAAIRFKNSMQENAKMQ
                     VMTEDVIEACHNGIVAWDKPKNVQPVLIQGKDDYVKTKERWEIIKELFRENGIQFKEI
                     FSQEGSILNKLVCLIYLLDYASIYNAIISKT"
BASE COUNT          370 a          188 c          128 g          432 t
ORIGIN      
        1 gttttagaaa ttattgcgtt gtatatgctt gcataatcaa gtaaataaat cagacaaacc
       61 aatttattca aaatacttcc ttcttgtgaa aaaatttctt taaattgtat accattttca
      121 cggaaaagtt ctttaataat ttcccatctt tcttttgttt ttacataatc gtcttttccc
      181 tgaattaaga caggctgtac attttttggt ttatcccatg caactattcc attatggcat
      241 gcttcaatca catcttctgt catcacctgc atttttgcat tttcttgcat tgaatttttg
      301 aatcttattg cagcagactg taaacccgac ggataataca ccataggtat ttctttaatc
      361 cattctgcaa tttgaagtgc ctcatttgtt tcattcaaat tttttgaaca aacagatttt
      421 tgtatattaa ataatgattc aattgattca ctgacatcag tttttttaac aggaacaatt
      481 tgttctaaaa tattaagtgt tgaatataag aatcctacaa atgatgctct aggcgaatgt
      541 tcctcctcaa ttttgtaata ttttatcttt ttattcatac aaaatttttc cataattccc
      601 cctgatgata atgccacaaa cttcgcattc attttttcta cattttttaa aattgaaatg
      661 gtttctaacg tatttccaga aatacttgtt gttacaacta gtgtactatc atctacagtt
      721 ttgggtaata gataaccttt aatcacagtt acatgaatat ttgtttttga aagaattgaa
      781 gcaaaaacat caccaatagt accagaacca cccattcctg caaaaactat atgatcaata
      841 ttttttatat caacttttga aaaatttacc gaaaaatttt ttctagcaat ttgaggccac
      901 ctatcataaa cctcagacat gctatctgaa tcaattgatt ttatatcatc tattttcaac
      961 aactagagaa agcatatgct tgatttataa aatcttggac acaatataat ttgatacaaa
     1021 cttttaatgg ttaaaactag attttttctt tatggatgat aatttgtcta taaatcttga
     1081 aaaatatgaa accaaaaata ctgttgatca acatgtta
//
LOCUS       DTVV01000061            1407 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1175824, whole genome shotgun sequence.
ACCESSION   DTVV01000061 DTVV01000000
VERSION     DTVV01000061.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1175824"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="EYO86_00760"
     CDS             <1..149
                     /locus_tag="EYO86_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010172731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIC41599.1"
                     /translation="ARERNIGWRIDYFFTNQEFANQIANADIHENVMGSDHCPIFLEL
                     SDNF"
     gene            complement(132..881)
                     /locus_tag="EYO86_00765"
     CDS             complement(132..881)
                     /locus_tag="EYO86_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41600.1"
                     /translation="MINQRLISLFFVLFFVNNVFAQDIYKSQKDIINLPDGLSTIVDN
                     KNKKAIIAIHGYYPPYWMRIHHEWVRPFNTLVEKNYRTFFYKYDWNRCPNDVGKEFAT
                     IITNLISENPDVEEWDIIGHSMGGIVTMFANKSFSPNNIVQFNIVATPLYIDIDKVPW
                     SSKNYYEKKFDRCPENLNSFRNEFDNGFPNPLIRWQNIKEFDSQFKKYDFNPYDREIK
                     DSVIFQFPDSLKNERVGHTASLYFSILEIIR"
     gene            905..1345
                     /locus_tag="EYO86_00770"
     CDS             905..1345
                     /locus_tag="EYO86_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIC41601.1"
                     /translation="MSFKIQLVKTEDHYQKILDVRRKVFIEEQNVSEEIEIEYEKEAV
                     HFLALDNNDCVGTGRWRKTDIGFKLERFAVLKHLRGKGVGKELVNFILNQLPTNQTIY
                     LHAQEDVIKFYQNLGFVIIGDKFLEADIVHSKMIYKQKNNKFIK"
BASE COUNT          502 a          182 c          220 g          503 t
ORIGIN      
        1 gtgctcgaga aagaaatatt ggttggagaa ttgactattt ttttacaaat caagagtttg
       61 ctaatcaaat agcgaatgct gatattcatg agaatgtaat ggggtcagat cattgtccta
      121 tatttttaga attatctgat aatttctaga attgaaaaat ataaacttgc agtatgtcct
      181 actctttcat tctttaggct atctggaaat tggaaaatca ctgaatcctt tatttctcta
      241 tcatagggat taaagtcata ttttttgaat tgtgaatcga attcttttat attttgccat
      301 ctaataagag gatttggaaa tccattgtca aattcatttc taaaactatt caaattttct
      361 ggacatctat cgaatttttt ttcgtaataa ttttttgaac tccaaggaac tttatcgata
      421 tcaatgtaaa gaggagtggc aacaatattg aattggacta tattgttagg actaaatgat
      481 ttatttgcaa acattgttac aataccaccc atactatgtc caatgatatc ccattcttcg
      541 acatcaggat tttcactaat tagattagta atgattgtag caaattcttt accaacatca
      601 ttgggacaac gattccaatc gtatttgtaa aaaaaagttc tatagttttt ttctaccaat
      661 gtattaaatg gtctaaccca ttcgtgatgt attctcatcc agtatggtgg ataatagcca
      721 tgaattgcaa tgattgcttt tttattttta ttatcaacaa tagtagaaag tccatctggt
      781 aaattgatta tatctttttg agatttataa atatcctgtg caaaaacatt atttacaaaa
      841 aatagtacaa aaaacagtga aattagtctt tgatttatca ttttataata ctgaaattgc
      901 gaaaatgagt tttaaaatac aattggtaaa aacagaggat cattaccaaa aaattttaga
      961 tgttagaaga aaggttttta ttgaggaaca gaatgtttct gaagaaatag aaattgaata
     1021 tgaaaaagaa gcagttcact ttttagcact tgataataat gattgtgttg gtacaggacg
     1081 atggagaaag acagatatag gttttaaatt ggaacgattt gccgttttaa agcatttaag
     1141 aggaaaaggc gttggaaaag agctcgtgaa ttttattcta aatcaattac ctactaatca
     1201 aacaatctat ctacatgccc aagaggatgt cataaaattt tatcaaaatt tgggatttgt
     1261 cattattgga gataaattcc ttgaggctga tatagttcat tcgaaaatga tctacaaaca
     1321 aaaaaataat aaattcatta aatgaaacaa aaatctcata ttcgtttgtt aaaaattgtt
     1381 tcagcttatt ggccactttt agtttta
//
LOCUS       DTVV01000062            1005 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1178658, whole genome shotgun sequence.
ACCESSION   DTVV01000062 DTVV01000000
VERSION     DTVV01000062.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1178658"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(366..947)
                     /locus_tag="EYO86_00775"
     CDS             complement(366..947)
                     /locus_tag="EYO86_00775"
                     /inference="COORDINATES: protein motif:HMM:PF14815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41602.1"
                     /translation="MKTLLRLNKKKIEDIASQYQSLNKASDLIQSFMDYGSAICLPRN
                     PKCDECLIEKFCEARKKNIQHLIPFKNLSLEKKKIKFTRAYIVVNNINEILVNRRKSS
                     GMLASMLEVPNDQWVNKKKLLERNAIYEKVGNKFKYKRAFTYSFSHFDLDIDIYKTVV
                     KKKMFKDHKWMKTNQIHISGMPTVMKQIIKKSR"
BASE COUNT          343 a          170 c          145 g          347 t
ORIGIN      
        1 aaagactaca tcacccttgc ccaaaaacga ttaaatgaaa cgcaagtctt aaaagataat
       61 ttagttactc tcactcaaag cagaaaagat ttaccaaaag ttccttttgg ggaattagtc
      121 gaacaaggaa ttattcctcc tggtgctgtg cttacggatg cgaaagaaag atttaaggcg
      181 actgtaacaa tagatggaag tttaaaaatc gatactcttt caggttccat tcaccaagta
      241 ggagcaaaat tacaaggatt acctagttgc aatggatggg atttttggca tgtaaaaaac
      301 aaaacttctt ctatcctctt agatcatgta cgtggtgatt accgaaataa aaaaacatta
      361 cttaattaac ggctcttttt aataatctgc ttcatcactg ttggcatacc gctaatatga
      421 atctgattag ttttcatcca tttatgatct ttaaacattt ttttcttaac taccgtctta
      481 taaatatcga tatctaaatc aaaatgactg aatgaatatg taaaagccct cttatattta
      541 aatttatttc ctactttttc ataaatagca tttcgttcta ataacttttt tttatttacc
      601 cattgatcat taggtacttc taacatggag gcaagcatac ccgaagattt acgtctattc
      661 accaaaattt cgtttatatt attcacaaca atataagcac gagtaaattt tattttcttc
      721 ttttctaaag ataaattttt aaaaggaata agatgttgaa tatttttttt tctggcttcg
      781 caaaattttt ctataaggca ctcatcacat ttaggattac gaggaagaca aattgccgaa
      841 ccataatcca tgaaagattg aattaaatca cttgctttat taagtgactg atattgagaa
      901 gcaatatctt caattttttt tttattgaga cgcagtaatg ttttcattcc atacagacgc
      961 gcaatgatcc tcttaatatt agcatctaaa ggcagaacag gttga
//
LOCUS       DTVV01000063            4976 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1179910, whole genome shotgun sequence.
ACCESSION   DTVV01000063 DTVV01000000
VERSION     DTVV01000063.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1179910"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(276..3866)
                     /locus_tag="EYO86_00780"
     CDS             complement(276..3866)
                     /locus_tag="EYO86_00780"
                     /inference="COORDINATES: protein motif:HMM:PF13289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41603.1"
                     /translation="MKIPATIKPYIEEIAQCLWSKNASIMIGAGFSMNAQPHFDNVKK
                     FPNWQELGNVFFRKVRGEDIQHAKYNFFDPLKLAYEVEANFGRAVLDNLLRENIPDSD
                     HKPSKLHYSLLNLPWTDVFTTNYDTLLERAADSVSERNYKVIVNKEDLIHSVSPRIVK
                     LHGCFAASTPLIINEEDYRTYPTKFAPFVNTVQQALLENTLCLIGFSGDDPNFLKWIG
                     WIRDNLGEKNSPKIYLIGVLGLTPSQEKTLSQYNITSVDLSLCEGVEGSHYNAIKLFI
                     DYCTKQKNSERVHWDINKGAYSKEINEKKIDFSNLHNELVTTVQTWQQDRQSYPGWLV
                     APKQYREVLWNRTSNWHSLFTLNSDELDFKLVFCFLYEFLWRKEKSLIPIFDHEVDFL
                     EKYLISWESYEDDAEVTREKAFFITIALLRYFREEGKNDSWLKFYRIAKSIAKTLDEK
                     ESLTNQYALFLLFENESVKLFDVLSKWNTESSSPYWMYRKASILAEVGECITAKENLE
                     KALIRVRKKINNSLIISSYSNVSLESYILTLLDEVCISIKFKDRVYEVENTDYSERLD
                     DLKQFECDPRLEKYLLTLNINHEPAILKSVTTKSGFDIGSKQTVQHYMHDNKEYIDAF
                     RFLKFCEDAGIPFRLPHVAIAKSGAQSAIKRLSFAAPYWSMVTMIRTHDEKSIELLFT
                     RECLSDYDLDFVDKLANKYLTLLNRYTTGKGCLYEYGLFLPEALSRMCSKSRIETRDR
                     ILELLILIYKQKNKNKNFSNINKLFKRLLNSYNHTELFERLPVLVDLSCELVSEDYSH
                     YDRYSFPNPISYISLKEDANFGGIKINPKTITSLIASLHSEVSEVRGEALITLNQLFT
                     LKILNKTQIKSFKTNLLKKLDNYGLPITNVFYKFYFLELFNCDKTLLRSFKEYLLSTS
                     PQSQSKQKEPKSFGLSDMPDAFTVELSGSFRYVNWEVEELEIHVKKILAWWESDKNIV
                     EKQQKADVFGFDVQKEMWVRFSKIVECLYKTVVNFELKKFRAQLSNMCFEFEEKGFNS
                     LYLKAVLLSYLELNATKFYNELGNALASEQKDIMLDAFRAINHLINVSGDYVKDILGL
                     LSNFMMFSNSSLIIHAIKISKVYFKKDKELFRLYLEASILNLLEKTDKGYEQFSFDER
                     LELRVSVGELACLIFNDYQENGQEIPSSILELRELQMSENEFSEVRNIWN"
BASE COUNT         1590 a          909 c          727 g         1750 t
ORIGIN      
        1 aaatcagata aatattggat tacaacagag tataaaaact tgaaaaagat aatgaaaata
       61 aagaaagtca cccaatgttg tttgaagtgt aagttgtcat agtcaaactc ggttttattg
      121 aaccagagcg gcgcaagtga agtataactt ggcatagaat tgaagcataa agtgacataa
      181 aatacctaaa actatggagt gctcgaaggc acccataatt tttgagttac ttacatatgg
      241 caccactgag ataccctata attactaggt gcgttttaat tccatatatt ccgaacttca
      301 gaaaattcat tttctgacat ttgcagctct cttagttcaa gtatcgagga tggtatttct
      361 tgtccattct cctggtaatc attgaatatt aaacatgcca actcaccaac cgaaacccta
      421 agctctaacc tttcatcaaa actgaactgt tcgtaacctt tatctgtctt ctctaaaaga
      481 ttaagaattg aagcttcgag atacaaccta aatagctctt tatctttttt gaagtaaact
      541 ttagaaattt taatagcatg aatgattaaa gaggagtttg aaaacatcat aaaatttgag
      601 agtaaaccta aaatgtcctt aacatagtca cccgatacat ttattaaatg gttgatggct
      661 ctaaaagcat ccaacataat gtccttttgc tcagatgcta atgcattacc tagttcatta
      721 taaaactttg tggcgtttag ttctaagtaa ctcaataaca ctgcttttaa atataatgaa
      781 ttaaaacctt tctcctcaaa ctcaaaacac atattactta attgagctcg aaatttttta
      841 agttcaaaat taacgactgt tttatataag cattctacaa ttttagaaaa acgcacccac
      901 atttcttttt gaacatcaaa accaaaaaca tctgcttttt gttgcttttc aacgatgttt
      961 ttatcggact cccaccacgc caatattttt tttacatgta tttccaattc ttcgacttcc
     1021 cagtttacat aacgaaaact tccggaaagt tctactgtaa atgcatcagg catatcactt
     1081 aaaccaaagg attttggttc tttttgtttt gattgagatt gaggagatgt gcttaacaaa
     1141 tattctttaa aactacgcag cagagtttta tcacaattaa ataactctaa aaaataaaat
     1201 ttataaaaaa cattggttat tggcaaacca taattatcta actttttcag caagttggtt
     1261 ttaaaagatt taatttgagt tttgttaaga atcttcaaag taaaaagttg atttaaggta
     1321 atgagtgctt cacctcttac ttcacttact tcagagtgca aagaagcaat caaagatgta
     1381 atagtcttag ggttaatttt tatcccccca aaatttgcat cttcttttag agatatatag
     1441 gatatcgggt taggaaaact atacctatca taatggctat aatcttcaga aactaattca
     1501 cagcttaaat ccaccaatac aggtagcctc tcaaacaact ctgtatggtt ataggaattt
     1561 aataatcttt taaacaactt attaatgttt gaaaagtttt tattcttatt tttttgttta
     1621 taaattaaaa tgagaagctc taaaattcta tcccttgttt cgatacgaga tttgctacac
     1681 attctagata gtgcctctgg taggaataat ccatactcat acaaacatcc tttcccagtt
     1741 gtataacggt ttaatagagt tagatattta tttgctagtt tatctacaaa atctaaatca
     1801 taatctgaca agcattctct ggtaaaaagt aactctatag atttttcatc atgagtcctt
     1861 atcattgtta ccatagacca gtatggtgca gcaaaggaga gtcgctttat tgcactctga
     1921 gctccacttt tagcgattgc gacatgtggc agccggaaag gtattcccgc atcttcacaa
     1981 aacttcaaga acctaaatgc atctatatat tccttattat catgcatgta atgctgcaca
     2041 gtctgcttac taccaatatc aaagccactt ttagttgtta ctgattttaa tatggctggt
     2101 tcgtggttaa tatttagagt gagtaaatac ttttctaatc tcggatcaca ttcaaattgt
     2161 tttaaatcat ctaacctctc gctatagtct gtattttcaa cttcatagac tctatcctta
     2221 aacttaattg aaatgcatac ttcatcgagc aatgttaaaa tgtatgactc tagggaaacg
     2281 tttgaataac tcgatattat taacgagtta tttatttttt ttcttactct aattaaagct
     2341 ttttctaaat tctcttttgc tgtaatacac tctccaactt ctgcaagaat gctagctttt
     2401 cgatacatcc aatatggtga gctcgattca gtattccact ttgataaaac atcaaatagt
     2461 tttaccgatt cattttcaaa caaaagaaat aaagcatact gattagtcaa gctttccttc
     2521 tcatctaatg tcttagcaat agactttgca attctataaa attttaacca tgagtcattt
     2581 ttaccctctt ctctaaaata tcttaataag gctatagtta taaaaaacgc tttctctctg
     2641 gttacttcag catcatcttc ataactttcc caagagataa gatatttttc taaaaaatca
     2701 acttcatgat caaaaatagg aattaacgac ttctcttttc tccataaaaa ttcataaaga
     2761 aagcaaaaaa ccagtttaaa atctagttcg tcactattaa gtgtaaataa tgagtgccaa
     2821 tttgaagttc tattccaaag aacctctctg tattgctttg gtgcaactaa ccagcctgga
     2881 tatgattgcc gatcttgttg ccaagtttga accgtagtga ctaactcatt atgtaaattg
     2941 ctaaaatcaa tttttttctc attgatttct ttagaataag cacccttgtt aatatcccaa
     3001 tgtacgcgtt ctgaattttt ctgtttagtg cagtaatcta taaaaagctt tatagcatta
     3061 taatgactcc cctcaacccc ttcacataat gataaatcaa cacttgttat attatattga
     3121 gaaagagttt tttcttgcga tggtgttaaa ccgagaaccc ctattaaata tattttaggg
     3181 gagtttttct cacctaaatt atctcttatc caaccaatcc atttcaaaaa atttggatca
     3241 tcaccagaga aaccaatcag gcacaatgta ttttcaagta gcgcttgctg aactgtattc
     3301 acaaaagggg caaattttgt tgggtatgtt ctgtaatctt cctcgtttat tattaaagga
     3361 gttgaagcgg caaaacaacc atgaagttta actattctag gagagacaga gtggataagg
     3421 tcttctttgt taactataac tttataattt ctttcactaa cagagtcagc cgctctctct
     3481 aacaaagtat cgtaattagt tgtaaaaaca tccgtccaag gtaaattcaa aagtgaataa
     3541 tgtaattttg atggcttgtg atcactatct ggtatatttt cacgcagtaa attatctaaa
     3601 acagctcttc caaaattagc ctcaacctca tatgctaatt ttagtggatc aaagaagtta
     3661 tattttgcat gctggatatc ttcccctctt actttacgaa aaaaaacatt acccagttcc
     3721 tgccaattcg gaaatttttt tacattatca aagtggggtt gagcattcat actaaaacca
     3781 gctccaatca taattgatgc atttttactc cataaacatt gggcaatttc ttcaatgtaa
     3841 ggcttgatgg tcgctggtat tttcataaat taattctcaa aattttaatc ttagtcgtag
     3901 ataactgtta agccgcataa gctacaataa acaaaacatg ctttacttct aaactgtgtc
     3961 atatcttcat ttccacagtg gcatttgagt tttttacatt tcgcatttag ctcagcaatt
     4021 aaaccttctt gaataactct tggctgtata tatttatcag ctacggatgc gtaagcagag
     4081 ttaccactta gtctattttg ccttttttca tctaaccttt ttctataatc atcgttctct
     4141 tgctggcgct gcctatatat ctgatcggcc tcgttttttg catcaatttc tgactgctga
     4201 acatcgaaat aggtaaggtt gttctcgtta aagtgtaagt cgttcaaact cactaatttt
     4261 gattgctctg aactgacaac ttgtacctga aagcctaaat catcgattcc gtattgtgta
     4321 taaaaacact taaaaaacag cttgctttga tctcggctag tgatttttga ttcaaggtca
     4381 aatacgtaaa ggttttcgtt agagttataa aatatgtctt ttcgggtgaa gttgtttttg
     4441 tcttgtagaa atagccaaag gactgtagca ccttgttctt taaaaaatcg atctctcgca
     4501 acaataacat ctagtggtac ttggtctaac actaactcaa tagctatatc catgttgtgc
     4561 gtgttagagt aaaaacttat atctggtctt cgccatgctt tagctagtcc cgtctgaaac
     4621 tgctctcgat aagtttgttc tacttgaata ttttcatact caaattcgct tttaagcaaa
     4681 tttgatatag tcgctttata gtctttatgt tttttacttt cttttattcc attgtaacta
     4741 attgccttta gttcactatc ggtgaggctt gttgtgcttt tcacaggaca atcttctgaa
     4801 ccgatagggt gtttaaaatg atgtttttgc gatgaagtac cggcaagtat taaaggctga
     4861 aaacatgtag cacatagtat acttccaagc ccttctggct ctcttagaaa gcgtctaagc
     4921 ttaaatagac ctgcttcatc tatagataga atttcattag aggaataact tttacc
//
LOCUS       DTVV01000064            1776 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1200673, whole genome shotgun sequence.
ACCESSION   DTVV01000064 DTVV01000000
VERSION     DTVV01000064.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1200673"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="EYO86_00785"
     CDS             <1..925
                     /locus_tag="EYO86_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC41604.1"
                     /translation="GEIYIGDQLVNEKDPKDRNVSMVFQNYALYPHMTVKENLGFSLK
                     IAKVDKKEIEKRVKEAVQILGLDELQKRKPSQLSGGQRQRVAMGRAIVRRPDAFLFDE
                     PLSNLDAKLRTQMRTEIKKLHQKLQTTIVYVTHDQVEAMTLADRIIIMKDGYIEQIGS
                     PIEVFNNPINVFVATFIGSPPMNILEVDIIKKDGKIYFELDQDVLMPCPESKASLLSQ
                     KNQVMIGIRSEDISPSNSNTSDEFWYFEKNIDLAEPLGTETQLFIKLKNKEIISRMYN
                     PREIKIGEQINFAVNLNKIHIFDNETKKIIK"
     gene            922..1575
                     /locus_tag="EYO86_00790"
     CDS             922..1575
                     /locus_tag="EYO86_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIC41605.1"
                     /translation="MNKVEIADRLQTCFSSVVHDVMRDEGLQSFTLPSSITPTKENYK
                     ISGQIFTIEGEINNKLSHHESLLAWTGFLSKAPKNKIIICQPNNQEIALMGELSAETL
                     QNKGVRGCIIDGGCRDLEFILNIDFPIWCNFYTPRDVVGYWSPTKMEEKITIGNTIIN
                     NNDYVMADIDGVVVIPEDKAEDILLKSEKLIATESEIRKAIREGMDPQEAYIKFSVF"
     gene            complement(1582..>1776)
                     /locus_tag="EYO86_00795"
     CDS             complement(1582..>1776)
                     /locus_tag="EYO86_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_437229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HIC41606.1"
                     /translation="FDRLRFPNPVFIGDTIHTKVTIKETKDDPKRPQYGQVIEACEII
                     NQNDKVVCYSEHILLVERKK"
BASE COUNT          679 a          236 c          309 g          552 t
ORIGIN      
        1 tggagaaata tacatagggg atcaattagt taatgaaaaa gatccaaaag acagaaatgt
       61 atcaatggta tttcaaaatt atgctcttta tcctcatatg acagttaaag aaaatctagg
      121 atttagttta aaaattgcca aagttgataa gaaggaaatt gagaaaagag ttaaagaagc
      181 tgttcaaata cttggtttag atgaattgca aaaacgaaaa ccttctcaac tttctggtgg
      241 tcaaagacaa agagtagcta tggggagagc tatagttaga cgcccagatg catttctctt
      301 tgatgaacca ttatctaatt tagatgcaaa acttagaacc cagatgagga cagaaatcaa
      361 aaaacttcac caaaaattac aaacaactat tgtatatgtt actcacgatc aagtagaggc
      421 aatgacccta gcagacagaa taattattat gaaggatgga tacattgagc agattggttc
      481 cccgatagaa gtttttaata accctattaa tgtatttgta gctactttta ttggaagtcc
      541 tccaatgaat attttggaag tagatattat aaaaaaggat ggaaaaatat attttgaact
      601 agatcaagat gtattaatgc catgtcctga atcaaaagcg agtcttctgt ctcaaaaaaa
      661 tcaagtcatg ataggaatta gatctgagga tatatctcct tcaaattcaa atacttctga
      721 cgaattctgg tattttgaaa aaaatataga tttagctgaa ccgctaggaa ccgaaaccca
      781 gttgtttata aaactaaaaa ataaagaaat tatcagtaga atgtataatc caagagaaat
      841 taaaattgga gaacaaatta actttgcagt aaatctcaat aaaattcata tttttgacaa
      901 tgaaaccaaa aaaattatca aatgaataaa gttgaaattg cagaccgtct tcaaacatgt
      961 tttagcagtg ttgttcatga tgttatgaga gatgagggat tgcagagttt tactttacca
     1021 tcttcaataa ctcccacaaa agaaaattat aaaattagtg gacagatttt tacaattgaa
     1081 ggagaaatta ataacaaact tagccatcat gaaagtttgt tagcatggac aggatttttg
     1141 tccaaagcac ctaagaacaa aataataatt tgccaaccta ataatcaaga aatagcttta
     1201 atgggagaac tttctgcaga aactttacag aataaagggg taagaggatg tataatagat
     1261 gggggctgta gagatttgga atttattctt aatattgatt ttccgatttg gtgtaatttt
     1321 tatacaccta gagatgttgt tggttattgg tcaccaacaa agatggagga aaaaattact
     1381 attggaaata caattattaa caataatgat tatgtaatgg cagatatcga tggagttgtg
     1441 gttattcctg aagataaagc tgaggacata ctcctaaaat ctgaaaaact tattgcaact
     1501 gaaagtgaaa ttagaaaagc aataagagaa ggtatggatc cgcaggaagc ttatattaaa
     1561 tttagtgtat tttaaatgtt attatttttt tctttctact aataaaatat gttctgaata
     1621 acaaacaact ttatcgttct gatttataat ctcacaagcc tcaataactt gtccatattg
     1681 tggtcttttt ggatcatctt ttgtttcttt tatcgttacc ttggtgtgaa ttgtatctcc
     1741 aataaataca ggattaggaa aacgtaatct atcaaa
//
LOCUS       DTVV01000065            1163 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1201147, whole genome shotgun sequence.
ACCESSION   DTVV01000065 DTVV01000000
VERSION     DTVV01000065.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1201147"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="EYO86_00800"
     CDS             <1..1115
                     /locus_tag="EYO86_00800"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038955.1"
                     /note="catalyzes the dehydration of
                     2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate
                     in valine and isoleucine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIC41607.1"
                     /translation="FTANTMACVSEALGLALPGSAGTPAVYEERDKFSVLSGEAVMHL
                     LEKKIRPRDIVTRKSLENAAVIVAASGGSTNAALHLPAIANEAGIKFTLEDVTNISKK
                     TPYIADLRPSGKYVAKDLYEIGGVPILIKALLEGGFLHENCMTVTGKTIAENHKDIIF
                     PSDQKIIYKTSKPINKSGGFVGLKGNLAPEGAIVKVAGMKRKKFKGKAKCFDAEKFAL
                     DAVLKRKIKPGDVVVIRYEGPKGSPGMPEMLSTTAAIYGQGLGEEVALITDGRFSGAT
                     HGFCIGHVGPEAANGGPISLIKNNDIIEIDAEKGSLKVQLSNKELIKRKKKWRPRKIE
                     YNSGTLWKYSQSVGPALKGAVTHPGAHKEKKSYADI"
BASE COUNT          430 a          163 c          244 g          326 t
ORIGIN      
        1 aatttactgc taatactatg gcatgtgttt ctgaagcatt aggtttagca ttacccggtt
       61 cagctggtac acctgcagtt tatgaagagc gtgataaatt ttctgtttta agtggagagg
      121 ccgtaatgca tttattagaa aaaaaaatta gaccaagaga tatagttact cgtaaatctt
      181 tagaaaatgc agctgtcata gtagctgcat ctggtgggtc caccaacgcc gctttacatc
      241 tacctgcaat tgcaaatgaa gcaggtataa aatttacact agaagatgtt acaaatattt
      301 caaaaaagac gccctatatt gctgatctta gacctagcgg gaaatatgtt gcaaaagatt
      361 tatatgaaat aggaggagtc ccaattctaa taaaagcttt attagaagga ggatttctgc
      421 atgaaaattg tatgactgtt actggaaaaa caattgcgga aaaccataaa gatattattt
      481 ttccatctga tcaaaaaata atttataaaa cctctaaacc tataaataaa agtggtggtt
      541 ttgtaggatt aaaaggaaat cttgcacctg aaggagccat tgtaaaagtt gctggcatga
      601 aaagaaaaaa atttaaagga aaggctaaat gctttgatgc tgagaaattt gcattggatg
      661 ctgttttgaa aaggaaaatt aaacctggag atgttgtagt gattaggtat gagggcccca
      721 aaggaagtcc aggtatgcca gaaatgttat ctaccactgc agctatttat ggacagggat
      781 taggtgagga agttgctcta ataacagacg gacgtttttc tggagcaaca catggatttt
      841 gtataggtca tgttggacca gaagctgcaa atggtggacc tataagttta attaaaaata
      901 atgacataat agaaattgat gctgaaaaag gctcgttaaa agttcaacta agtaataaag
      961 agttgattaa aagaaaaaaa aagtggagac caagaaaaat tgaatataat tctggtacat
     1021 tatggaaata ttcccaatct gttggtccag cacttaaagg agcagtcacg catccaggtg
     1081 cacacaaaga aaaaaaatca tacgctgata tataaaaagt aaatgaaaat caaaccagtt
     1141 attttatgtg gtggtggagg tac
//
LOCUS       DTVV01000066            1290 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1201507, whole genome shotgun sequence.
ACCESSION   DTVV01000066 DTVV01000000
VERSION     DTVV01000066.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1201507"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="EYO86_00805"
     CDS             <1..961
                     /locus_tag="EYO86_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC41608.1"
                     /translation="IIECREAVTRKIKSLYKVDIDPERVIIMPGGKPTMYYAITLFGE
                     PGAEIIYPDPGFPIYESMINYTGAKAVPINILENKDFSMNPQKILSLINDKTRLIILN
                     NPNNPTGGFTGKNKINQLAEGLKKFPHVAILSDEIYSRQIFDQKKIPTFFDYPDLYNR
                     LIVLDGWSKAYSMTGWRLGWGVWPEHLVEHVIKFCVNNHSCVNAAVQFGGIAALDGPD
                     NSIMMMMDKFTKRRKLIFEGLNSINGIECSLPGGAFYAFPKVIGTGMNGQEFAKKCLH
                     EAGVAIVPGTAFGKKAADYVRFSFAASQEDISKALEKIRKMLV"
     gene            970..>1290
                     /locus_tag="EYO86_00810"
     CDS             970..>1290
                     /locus_tag="EYO86_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC41609.1"
                     /translation="MNNIALPKIDQSIIERKKYIFSKLKKLTATENFLSHEAEIKPYE
                     TDALAAYKQMPLGVILPESTEEVSNILKFCYQENIKVVPRGAGTGLSGGALPLQDAIL
                     ISLTK"
BASE COUNT          487 a          175 c          236 g          392 t
ORIGIN      
        1 aataattgaa tgtagagaag cagtcactag aaaaataaag agtctttaca aagtagatat
       61 tgatcctgaa agagtaatta ttatgcctgg tggaaagccc acaatgtatt atgcaataac
      121 attattcggc gaaccaggag cagaaataat atatcccgat cctggttttc caatttatga
      181 gtcaatgata aattatacgg gagcaaaagc agttccaatt aatattcttg aaaacaaaga
      241 tttttccatg aatccacaaa aaatattatc actaattaat gataaaactc gattaataat
      301 tttgaacaat cctaacaatc caactggtgg atttactggc aaaaataaaa ttaatcaatt
      361 agctgaaggc ctaaaaaaat tcccacatgt tgcaatttta agtgatgaaa tttatagcag
      421 acaaattttt gatcaaaaaa aaataccaac tttttttgat tatcccgatc tatataacag
      481 attaattgtt cttgatggtt ggagcaaagc ttattcaatg actggatggc gtttaggatg
      541 gggtgtgtgg ccagaacatc tagtagaaca tgtaattaaa ttttgtgtta ataatcattc
      601 atgtgtaaat gcagctgttc aatttggagg aattgcagca ctagatggac ctgacaattc
      661 aataatgatg atgatggata agtttacaaa aagaagaaaa ctaatttttg aaggactaaa
      721 tagtattaat ggaattgagt gtagtttgcc aggtggagca ttttatgctt ttccaaaagt
      781 tattggcact ggaatgaatg gacaagagtt tgcaaaaaaa tgtcttcatg aagcaggtgt
      841 agcaattgtt ccaggaactg catttggtaa aaaagcagct gattatgtaa gatttagttt
      901 tgctgcttcg caagaagata tttctaaggc tttagaaaag ataagaaaaa tgttagttta
      961 gtagacctaa tgaataacat agctttacct aaaatagacc aatctatcat agagagaaaa
     1021 aaatatattt tcagcaaatt aaaaaaatta acggctactg aaaatttttt aagtcatgaa
     1081 gctgaaataa agccttatga aactgatgca ctagcagctt ataagcaaat gcccttaggc
     1141 gttatattgc ctgaaagcac agaagaagta agcaatattt taaaattttg ttatcaagaa
     1201 aatattaaag ttgttccaag aggtgccgga actggtttgt caggaggagc attacctctt
     1261 caagatgcta ttcttattag tcttacaaaa
//
LOCUS       DTVV01000067            1334 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1203858, whole genome shotgun sequence.
ACCESSION   DTVV01000067 DTVV01000000
VERSION     DTVV01000067.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1203858"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="EYO86_00815"
     CDS             complement(<1..815)
                     /locus_tag="EYO86_00815"
                     /inference="COORDINATES: protein motif:HMM:PF00342.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41610.1"
                     /translation="MTNLNINYHNVDFSRHLVLPSNNDSIISQTQNILKDLPSLNIVH
                     NNKLLNQTIQQATHFANGKENFIVFGTGGSNLGSQALINILQGHEKNKIIFHDNIDPI
                     NFQNSIDTINLNTTGFIVISKSGSTPETLSQFASIIEIFDQKNNLKALFSNVLIITED
                     KISPLSQIAHANKCTILKHEKSIGGRYSIFSNVGMIPAIIAGLDVKQIHAGALSVIKD
                     QQGKENYKIAQFFRYQNSHYSLTNSVILTYSDALYYFGKWYLQLWAESLGKNK"
     gene            complement(820..>1334)
                     /locus_tag="EYO86_00820"
     CDS             complement(820..>1334)
                     /locus_tag="EYO86_00820"
                     /inference="COORDINATES: protein motif:HMM:PF11233.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HIC41611.1"
                     /translation="LSVVLTLNACSNVRDSAGVTRKSIDEFQVIENPPLIIPPDFNLL
                     PPDQLEEKNIDDEEKELAQEILFGLDENEIQTVTQLSTMNQILSQADALDASSSIREE
                     LDREFAQEIKTDGIFQINWENEVEVLDAIKESERIRNKNFEGESIAEGDVPIKTKKIK
                     IKKKKRFFFF"
BASE COUNT          414 a          214 c          201 g          505 t
ORIGIN      
        1 ttcttatttt ttcctaaact ttcagcccat aattgtaagt accatttccc aaaataatac
       61 aatgcatcgg aatacgtcaa aattacactg tttgttaatg aataatgaga attttgataa
      121 cgaaaaaatt gtgctatctt ataattttcc ttaccctgct ggtctttaat aacagataat
      181 gctccagcgt gtatctgctt aacatccaag cctgcaataa tagctggaat cattccaaca
      241 tttgaaaaaa ttgaatagcg tcccccaata ctcttttcat gtttcaatat cgtgcattta
      301 ttagcatgcg ctatctgaga taaagggcta attttgtctt ctgtaatgat caatacatta
      361 gaaaataaag ccttaagatt atttttctga tcgaatattt ctattatact tgcaaattga
      421 cttagtgttt caggtgtaga tcctgactta gagataacaa taaaacctgt cgtatttaga
      481 ttaattgtat caattgaatt ttgaaaattg atggggtcaa tattatcatg aaaaataatt
      541 ttgttttttt catgaccttg taaaatatta atgagggctt gagatcctaa gttggagccg
      601 ccggttccaa atacaataaa attttctttt ccatttgcaa aatgagtagc ctgttgaatg
      661 gtttgattta acaacttatt attatgaaca atatttaaag aaggtaaatc ttttaatata
      721 ttttgcgttt gagagataat gctatcatta ttgcttggta atactagatg ccgactgaaa
      781 tccacattgt ggtaattaat atttaaattt gtcatatagt taaaaaaaga agaatctttt
      841 tttctttttg atttttattt tttttgtttt aatagggaca tctccctccg ctattgattc
      901 tccttcaaaa tttttattgc gtatgcgctc tgattcctta attgcatcta atacttccac
      961 ctcgttttcc caattaattt gaaaaatacc gtcagtttta atctcttggg caaactctct
     1021 gtctaattct tcacggattg aagaagatgc atctaacgca tcggcttgag ataaaatttg
     1081 gttcatggtt gataattgag ttacagtttg tatttcattt tcatctaatc caaaaagtat
     1141 ttcctgagct aattcttttt cttcatcatc aatatttttt tcttctaatt gatcgggcgg
     1201 caaaagatta aaatcagggg gaatgattaa aggtgggttt tcaattactt gaaattcatc
     1261 tattgatttt cttgtgacac ccgcactatc acgcacgttt gagcacgcat ttagtgttaa
     1321 aacaactgaa agaa
//
LOCUS       DTVV01000068            1403 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1209099, whole genome shotgun sequence.
ACCESSION   DTVV01000068 DTVV01000000
VERSION     DTVV01000068.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1209099"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="EYO86_00825"
     CDS             complement(<1..511)
                     /locus_tag="EYO86_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIC41612.1"
                     /translation="MIKQQIPVLPLRDIVVFPHMVAPLFVGRQPSVKALNNVMTKDKK
                     ILLITQRNSEVDSPKPENLYKCGTLAKVLQLLKLPDGTIKVLVEGFERVRILSIEDSG
                     DFLRASYSILSVKEKNTANIMALAKIIKDQFESYQKINKKISIDLVNNIKTYNDFNKL
                     ADVVIANLNI"
     gene            complement(543..>1403)
                     /gene="clpX"
                     /locus_tag="EYO86_00830"
     CDS             complement(543..>1403)
                     /gene="clpX"
                     /locus_tag="EYO86_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIC41613.1"
                     /translation="FTMADATSLTEAGYVGEDVENIILKLLQASDYNVERAQKGIVYI
                     DEIDKIGRKSDNPSITRDVSGEGVQQALLKIMEGTVASVPPQGGRKHPQQEFLHIDTS
                     NILFICGGAFTGLEHIINSRLKGSSIGFNATLSNYSDKKIGESLINLQNEDLLKFGMI
                     PEFIGRLPVCTTLHELNEKMLVRIMKEPKNAIIKQFEVLFKMDNIALEIKNEALDEIA
                     KLAVLRKTGARGLRSIMEDLLVDLMYETPDQTELKKIIINRDVLIKKTKPMLLFTHKD
                     NNRKIAVNKS"
BASE COUNT          442 a          238 c          174 g          549 t
ORIGIN      
        1 taatattaag gtttgctatt acaacgtcag caagtttatt aaagtcattg taagttttga
       61 tattatttac aaggtcaata gaaatttttt tatttatttt ttgatatgat tcaaattgat
      121 ctttaattat ttttgcaaga gccattatat ttgctgtgtt tttttcttta acacttaaaa
      181 tagaatatga tgctcttaaa aaatctcctg aatcttctat tgataaaatt ctaactcttt
      241 caaatccttc aaccaaaacc ttaattgttc cgtcaggtag ctttaacaac tgaagtactt
      301 ttgctaatgt cccgcattta tacaaatttt ctggtttagg actatcaact tctgaattcc
      361 tttgtgttat tagcaatatc tttttatcct ttgtcataac attgttaaga gcttttacag
      421 atggttgtct acctacaaat agtggagcta ccatatgagg aaaaacaact atgtcacgaa
      481 gtggaagaac tggtatttgt tgcttaatca taattattta aatgattata ataattcata
      541 aatcaagact tatttactgc tatttttcga ttattatcct tatgggtaaa taacaacatt
      601 ggttttgttt ttttaattag aacatctcta ttaataataa tcttttttag ctctgtttga
      661 tcaggtgttt catacattaa atctactaat aaatcttcca ttatagatct aaggcctcta
      721 gccccagttt ttcttaagac tgccaattta gcaatttcat ctagggcttc atttttaatt
      781 tccaatgcaa tattatccat tttaaataag acttcaaact gcttgataat agcatttttt
      841 ggttctttca taattcgtac aagcattttt tcatttaatt catgcaaagt agtacatact
      901 ggtaaacgtc caataaactc aggtatcatt ccaaatttca ataaatcttc attttgtaag
      961 ttaataagag attctccaat ttttttatca ctataatttg atagggtagc attgaaacca
     1021 attgaagagc ctttcaggcg actattaata atatgttcta aaccagtaaa agcacctcca
     1081 caaataaata atatattaga tgtatcgata tgtaaaaatt cttgttgagg atgttttctt
     1141 cctccctgag gtggaacact tgctacagtt ccttccataa ttttaagcaa tgcttgctgt
     1201 acaccttctc cagaaacgtc cctagtaata gatggattgt cactctttct accaatttta
     1261 tctatctcat ctatgtaaac aatacctttt tgcgctcttt caacattata atcagaagct
     1321 tgcaataatt ttaatataat attttcaaca tcttcaccca cataaccagc ttctgttaaa
     1381 ctagttgcgt ctgccattgt aaa
//
LOCUS       DTVV01000069            1420 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1213500, whole genome shotgun sequence.
ACCESSION   DTVV01000069 DTVV01000000
VERSION     DTVV01000069.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1213500"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="EYO86_00835"
     CDS             <1..248
                     /locus_tag="EYO86_00835"
                     /inference="COORDINATES: protein motif:HMM:PF07977.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HIC41614.1"
                     /translation="ALAQTAGVVVSTNFEKNSNKSVLFLSISKAKFRKPVVPEDTIEL
                     HVEYINKVKSVYKFKGVASKSEIIVCEAEFSAMITSG"
     gene            315..1100
                     /gene="lpxA"
                     /locus_tag="EYO86_00840"
     CDS             315..1100
                     /gene="lpxA"
                     /locus_tag="EYO86_00840"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIC41615.1"
                     /translation="MIHKTAIISASCQIHDDVIIGPYCIVDENVSIGEGTELISNVHI
                     SGNTKIGKNNKFFPFSSIGMIPQDKKFTGEDSQLIIGNDNVIREHVTINPGTRKGGMK
                     TLIKNKCLFMVGSHIAHDCQISSNVILVNNATLGGHVNIEEGAIIGGNSGVHQFVNIG
                     KFAMIGGMSGVGENIIPYALYTGVRSNLRGLNLIGLKRKKISSIQINMIKKIFKKIFN
                     SNFTIFENIKNLKKEERNIDQIKEIINFITSNYKRGICKYIDD"
     gene            1093..>1420
                     /locus_tag="EYO86_00845"
     CDS             1093..>1420
                     /locus_tag="EYO86_00845"
                     /inference="COORDINATES: protein motif:HMM:PF06230.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1009 domain-containing protein"
                     /protein_id="HIC41616.1"
                     /translation="MTKIAIISGEGQLPLLIGKNLINKKFNILFICLKDFADPLLYKK
                     FNFLEITITSFSKILKALQKEKVDEIIMVGKISRFNILDINFDLNTLGLIKKYFLESK
                     GDDKLLT"
BASE COUNT          570 a          140 c          214 g          496 t
ORIGIN      
        1 aggcccttgc acaaactgca ggtgttgttg tatctacaaa ttttgaaaaa aattctaaca
       61 aatctgtttt gtttctgagt atatcaaagg ctaaattccg aaaacctgtt gttcccgaag
      121 atacaattga attgcatgtt gaatatatta ataaagtaaa atctgtatat aaatttaaag
      181 gagtagcatc taaatcagaa attattgttt gcgaagcaga attttctgca atgattacta
      241 gtggctgaat attggaaata ttaagtaaca atatattact gtaattattt tataataatg
      301 attattcata atcaatgatt cataaaacag caattatttc cgctagttgt caaattcatg
      361 atgatgtaat tattggacca tattgcatag ttgatgaaaa tgtttcaata ggtgaaggaa
      421 cagagttaat ttcaaatgta catataagtg gcaatacaaa aataggtaaa aataataaat
      481 ttttcccttt ttcatctatt ggaatgattc ctcaagataa aaaatttaca ggcgaagatt
      541 ctcaattaat tatcggaaat gataatgtta tcagagaaca tgtaactatt aatcccggaa
      601 ctagaaaagg agggatgaaa actttgataa aaaataaatg cttgtttatg gtaggttcac
      661 atatagcaca tgattgtcaa atttcttcta atgtaatctt ggttaataat gcaaccctag
      721 gagggcatgt aaatattgaa gaaggtgcaa ttataggggg caattctgga gttcatcaat
      781 ttgtaaatat tggtaaattt gccatgattg gtggaatgag tggtgttgga gagaatatta
      841 ttccctatgc tttatatact ggtgttagat ctaatttgag aggtttgaat ttaataggat
      901 taaaaagaaa aaaaatttct tcaattcaaa taaatatgat taaaaaaatt tttaaaaaaa
      961 tttttaattc aaattttaca attttcgaaa atattaaaaa tttaaaaaaa gaagaaagaa
     1021 atattgatca aattaaagaa ataataaatt ttattacttc aaattacaaa agaggaattt
     1081 gtaaatatat tgatgactaa aatagctata atttctggtg aaggacaatt gcccctactt
     1141 attggaaaaa atttaataaa taaaaaattt aatattttat ttatatgtct aaaagatttt
     1201 gcagatcctt tattatataa aaagtttaat tttttagaaa ttactataac atctttttca
     1261 aagattttaa aagcattaca aaaagaaaaa gtagatgaaa ttataatggt tgggaaaata
     1321 tcaagattta acatattaga tattaatttt gatttaaata ctttaggtct tattaaaaaa
     1381 tattttttag aatctaaggg tgacgataaa ttattaacca
//
LOCUS       DTVV01000070            1039 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1214661, whole genome shotgun sequence.
ACCESSION   DTVV01000070 DTVV01000000
VERSION     DTVV01000070.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1214661"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="EYO86_00850"
     CDS             complement(<1..424)
                     /locus_tag="EYO86_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC41617.1"
                     /translation="MKQIKNIGFWFLVLFLLLLICLPGIWIFVSAFRPNHEILSKPAN
                     WIPDRWTIFEFTQMFSIKGYVSVPAFQYLMNSIIISLFSTIIAITIGMMGGYAFARFN
                     FKGKNKIFLGLMLARAVPGISLSLPLFILFAKLGIINTS"
     gene            complement(424..>1039)
                     /locus_tag="EYO86_00855"
     CDS             complement(424..>1039)
                     /locus_tag="EYO86_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_437223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC41618.1"
                     /translation="LINKITWGQRTLRTIMMFPMMFSPILVGFQWKYIFNDNIGLLNN
                     FLYLFGYESAIAWLIDKKLAMGSIMVAEIWANTAIFSILLLAGLIGIPRDPIEAARVD
                     GCNSWQVFKHITLPFLMPFIFIALTIRSLDVGRAYDIVHIMTGGGPAMRTELIWTMIA
                     RVGYKDANMGYANAMSYVSIILSIAFTYYFFTKLVAARRELGQQ"
BASE COUNT          387 a          183 c          143 g          326 t
ORIGIN      
        1 aggaggtgtt tataattcct agcttagcaa aaagaataaa tagaggtaaa cttaaagaaa
       61 ttccaggcac tgctcttgca agcattagtc caaggaaaat tttattttta cctttaaaat
      121 taaatctagc aaaagcatac ccgcccatca tacctattgt tattgcaatg attgtactaa
      181 aaagagaaat aataattgaa ttcattaaat actgaaaagc aggaactgaa acgtaacctt
      241 taatactaaa catttgtgta aactcaaata ttgtccatct atctggtatc caattagcag
      301 gttttgataa tatttcatga ttaggacgaa atgcacttac aaaaatccaa attcctggta
      361 agcaaattaa tagtaaaaga aataaaacta aaaaccaaaa cccgatattt tttatttgtt
      421 tcattattgt tgccctaact ctcttctagc tgctacaagt ttagtaaaga agtaataagt
      481 aaaagctata gataaaataa ttgatacata tgacatagca ttagcataac ccatgttcgc
      541 atctttgtag cctacccttg caatcatagt ccaaattaat tctgttctca ttgcaggacc
      601 tccgccagtc ataatgtgaa caatgtcata tgctcttcca acatcaagag acctaattgt
      661 tagagctata aatataaatg gcattaaaaa aggaagggta atgtgtttaa atacttgcca
      721 actattacaa ccgtctactc gtgccgcttc aattggatct cttggaatac ctataagacc
      781 tgctaataat aatatggaaa atattgctgt atttgcccaa atttcagcaa ccataattga
      841 tcccattgca agttttttat caataagcca tgctattgca gactcatacc caaagagata
      901 taaaaaatta tttagtaaac caatattatc attaaagatg tatttccatt gaaagccaac
      961 caaaattggc gagaacatca ttggaaacat cattatagtt cttaatgttc tttgacccca
     1021 tgttatttta ttaatcaaa
//
LOCUS       DTVV01000071            1202 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1215094, whole genome shotgun sequence.
ACCESSION   DTVV01000071 DTVV01000000
VERSION     DTVV01000071.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1215094"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1202
                     /locus_tag="EYO86_00860"
     CDS             <1..>1202
                     /locus_tag="EYO86_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HIC41619.1"
                     /translation="EIVSFDKDGKNELGIIGRQKNANANFIKYILTNSPDNKFLISKL
                     KKRKIDFHNANSCNLFNSETVSGETEELTALENGFIIIAAPGKSMLVDKQEVASDLEI
                     KVLRKNINNKKLDYFLPDPLSDTKEEYLIKDSTALAYEVKEGDFIQVIDIYGQQCSDF
                     MAFNSPLLQKGKEFSIDARVTRSIVGGAYPMPGLFAKYFDKNQDTLVEVIQDTCGRHD
                     TFGTACTLKYYEDMGYFGHPNCSDNYNGQLEPFGVEKRKGWQAINLFFNTSINTTNVL
                     FSDIPWSRPGDYVLFQAQKDLVCVSSACPCDVDAANDWNPTDVYVRVYSKKKIFSKAT
                     GYRKNANSDFILTKQTAFHERTSVMTKDMMDSAGFWIPNKYNNYGTIEEYTACRNNVV
                     VMDLSSLR"
BASE COUNT          419 a          190 c          219 g          374 t
ORIGIN      
        1 gaaattgttt cttttgacaa ggacggtaaa aatgaacttg gtattattgg tcggcaaaaa
       61 aatgcgaatg caaattttat aaaatacatt ttaactaata gtccggataa taaattttta
      121 atttcaaaat taaagaaaag aaaaattgat tttcacaatg caaattcgtg caacttattc
      181 aattctgaaa cagtatctgg tgaaactgaa gaattaacag ctcttgaaaa tggatttatt
      241 atcattgctg cacccggaaa atcaatgctt gttgataaac aagaggtagc ttctgatcta
      301 gaaatcaaag ttctaagaaa aaatattaat aataaaaaat tagattattt tttgccagac
      361 cctctatctg atacaaaaga ggaatattta atcaaagaca gtaccgcctt agcctatgaa
      421 gtaaaggagg gtgatttcat tcaggtaatt gatatctatg gacagcaatg ttctgatttt
      481 atggccttca attctcctct tttgcaaaaa gggaaagaat tttcgattga tgcgagggta
      541 actcgaagca ttgtgggtgg tgcttaccca atgcctggtt tattcgccaa atattttgac
      601 aaaaaccaag atacattagt tgaagtaatc caagacacct gcgggcgtca tgatacgttt
      661 ggaactgcat gcactttaaa atactatgaa gacatgggat attttggcca cccaaattgt
      721 tcagataatt ataacggtca gctagagccc tttggagttg agaaaagaaa aggttggcaa
      781 gctatcaatc tttttttcaa tacttcgata aatactacaa acgtattatt ctcagatata
      841 ccttggtcaa ggcctggtga ttatgttttg tttcaagcac aaaaagattt ggtctgtgta
      901 tcttccgcat gtccctgtga cgttgatgct gcaaatgact ggaatcctac tgacgtttac
      961 gttagagtct attcgaaaaa aaaaattttt tctaaagcta caggttatag aaaaaatgca
     1021 aactcagatt ttattttgac caaacaaaca gccttccatg aacgaacatc tgtcatgaca
     1081 aaagacatga tggactcagc tggtttttgg attccgaata aatacaataa ttacggaaca
     1141 atagaagaat atactgcttg taggaataac gttgttgtaa tggatttatc atctcttcgt
     1201 aa
//
LOCUS       DTVV01000072            1711 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1218424, whole genome shotgun sequence.
ACCESSION   DTVV01000072 DTVV01000000
VERSION     DTVV01000072.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1218424"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(19..570)
                     /locus_tag="EYO86_00865"
     CDS             complement(19..570)
                     /locus_tag="EYO86_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIC41620.1"
                     /translation="MGKKYSEIPNNLTQFIESQKIFFVGTAAADGRVNISPKGMDSFR
                     ILDANRVLWLSVTGSGNETSAHVQENPRMTIMFIALEGSPMILRLYGSARVIHKSDSD
                     WEECASLFELLPGTRQIFDLTIDLVHTSCGMGVPFYNYTGERDQLNAWANKKGEEGIK
                     AYWREKNEESLDGKITHIIEKNI"
     gene            complement(595..804)
                     /locus_tag="EYO86_00870"
     CDS             complement(595..804)
                     /locus_tag="EYO86_00870"
                     /inference="COORDINATES: protein motif:HMM:PF13619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HIC41621.1"
                     /translation="MIEWVTVFTSDIKKIGYDSATNKLHIDFKDDSDTYSTYCNVPQD
                     LYYQFVSASSVVHFYNQTIKDKYDC"
     gene            1203..1538
                     /gene="tusE"
                     /locus_tag="EYO86_00875"
     CDS             1203..1538
                     /gene="tusE"
                     /locus_tag="EYO86_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIC41622.1"
                     /translation="MEVNDEIIELDNEGYLIEPSQWQRDVAIQLATEENLELNDEHWI
                     VLGLMRDFYNENGIAPDVRHIFKQLGVDLDISKKEAKAKLFSLFPYGYVQQACKISGM
                     RRPRGWSTG"
     gene            complement(1541..>1711)
                     /locus_tag="EYO86_00880"
     CDS             complement(1541..>1711)
                     /locus_tag="EYO86_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020024884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HIC41623.1"
                     /translation="QTAGTRGKSLIINLPGKPAAIAVCLGAVFLAVPKCLELLDDSNI
                     QIDLDFVEQDFS"
BASE COUNT          534 a          312 c          287 g          578 t
ORIGIN      
        1 agactaaggt ctgactatct agatattttt ttcaataata tgggtaatct taccatcgag
       61 actttcctca tttttttctc gccaataggc ttttatacct tcttcgcctt ttttatttgc
      121 ccacgcattt aactgatccc tttcaccagt gtaattataa aatggtacgc ccataccaca
      181 ggatgtgtgg acaagatcaa tagttaaatc aaagatttgt cttgtaccgg gtagtaattc
      241 gaatagactg gcacattctt cccagtctga gtcacttttg tgaattactc tagcactacc
      301 ataaagcctt aaaatcattg gactgccctc taatgcaata aacattattg tcattctagg
      361 attctcctga acatgagctg atgtttcatt tccactacca gtaacactca gccataaaac
      421 acgatttgcg tctaaaatac gaaatgaatc catgcccttt ggcgatatat tgactctgcc
      481 atcagcagct gcagtaccaa caaagaatat tttttgactc tcaataaact gcgtcagatt
      541 attggggatt tcggaatatt tttttcccat tgtttttatt agttttttgt tctgttaaca
      601 atcatattta tctttaatag tctggttgta gaaatgaact acagaacttg cagacacaaa
      661 ttggtagtat aaatcctgag gcacgttaca ataagtggag taggtgtcac tatcatcctt
      721 gaaatcgatg tgcaacttgt ttgtggctga gtcataaccg atttttttaa tatcgcttgt
      781 aaatacagta acccattcaa tcataattat tctcctgtta atttaaattg tattttagtt
      841 aaaaactgag cttattgaaa gttaactgcg tctgattatt aaggatttca gaatattttt
      901 ttcccataaa tttacagcct ttcgtttagt aaaatattca ggtctcgatt gtaaagaaaa
      961 acgctttatt gtgaatatat actaatttat attcgccaat atattttgca ttacggattc
     1021 atccggcttt ataatataac cggtttcacc tttatttaag tataaggctt accatcagaa
     1081 aataaatctt aatctttaaa gattatttta ctcttaacca ctattgtcat tttatcattt
     1141 ggatgagaat actgaagtat gcctatttat acataattat ataaaaattc tacagggaaa
     1201 taatggaagt taatgatgaa ataattgagc ttgataatga aggctactta attgaaccta
     1261 gccaatggca gcgtgatgtt gcaattcaat tagcaacaga agaaaatcta gagcttaatg
     1321 acgaacattg gatcgttctt ggtttaatgc gtgattttta caatgaaaat ggaatcgcac
     1381 ctgatgtgcg tcatattttc aagcagttag gtgtagattt ggacattagt aagaaagaag
     1441 ctaaagctaa attattctcc ttgttcccct atggctacgt acagcaagct tgtaagatct
     1501 caggaatgag acgaccaaga ggctggagta ctggctaaaa ttaagagaag tcttgctcta
     1561 caaaatccaa gtcgatttgg atattagaat catctagcaa ttccaggcat tttggtaccg
     1621 ctaagaatac cgcgcccaaa cacacggcaa tcgcagctgg cttgccaggc aagttaataa
     1681 ttaaactttt accacgcgta cccgctgtct g
//
LOCUS       DTVV01000073            1050 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1218770, whole genome shotgun sequence.
ACCESSION   DTVV01000073 DTVV01000000
VERSION     DTVV01000073.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1218770"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="EYO86_00885"
     CDS             complement(<1..162)
                     /locus_tag="EYO86_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative cytochrome c oxidase polypeptide
                     CoxN"
                     /protein_id="HIC41624.1"
                     /translation="MRKKEGIFQLLAQKPWDPAQAKIDDMHEGGTIDLSKGKLGLRFI
                     MLVSTIFFCL"
     gene            complement(171..>1050)
                     /locus_tag="EYO86_00890"
     CDS             complement(171..>1050)
                     /locus_tag="EYO86_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIC41625.1"
                     /translation="HARKNIFGYRMMVWAIVIIGALSFIVWAHHMYVSGMNPWFGFFF
                     ATTTLIIAVPTALKVYNWVITLWKGNIRLTTPMLFALAFIVTFVNGGITGLFLGNVIV
                     DVPLSDTYFVVAHFHMVMGIAPILVIFGAIYHWFPLITGRYLHEGMGKFHFWVTFLGS
                     YLIYFPMHYLGFVGVPRRYYEMYESPYITASTIPLNQFITIAALAVGLTQLLFLYNII
                     TSAKLGKKAERNPWGACSLEWQTPDVPATHGNFGKELPVVYRWAYDFSVPGAKEDFIP
                     QNVPPSEVPDAEVEKT"
BASE COUNT          360 a          209 c          177 g          304 t
ORIGIN      
        1 taaacaaaag aaaattgtac tgacaagcat aataaaccta agtccaagct ttcctttgct
       61 taaatctatt gtgccacctt catgcatgtc gtcaatttta gcctgagcag ggtcccacgg
      121 cttttgagct aatagttgaa atataccttc tttttttctc atgttttttc ttatgttttt
      181 tctacttctg catcaggtac ttcgcttgga ggaacattct gcggaatgaa gtcctctttt
      241 gctcctggga cactgaaatc ataagcccaa cgataaacaa caggtaattc tttcccaaag
      301 tttccatgtg ttgcaggtac gtcaggtgtt tgccactcta atgaacaagc tccccaagga
      361 tttctttcag cctttttacc taactttgcg ctagtaatga tattatataa aaaaagtaat
      421 tgggttaaac ctacagctaa agccgcaatg gtaataaatt ggtttaacgg aatagttgaa
      481 gcagtaatat aaggactttc atacatctca tagtatcttc tgggaactcc cacaaatcct
      541 aaataatgca ttggaaaata tattagatag gatcccaaga atgtaaccca aaaatgaaat
      601 tttcccattc cttcatgtaa atatcggcca gtaattagag ggaaccaatg atagatggct
      661 ccaaatataa caaggatagg ggctatcccc atgaccatgt gaaaatgtgc tactacaaaa
      721 tatgtatcag atagagggac atcaacaata acattcccta aaaataaacc tgtaattcca
      781 ccgttaacaa aagtaacaat aaatgcgaga gcaaaaagca tcggggtagt taaacggata
      841 tttcccttcc ataatgttat aacccaattg taaactttaa gtgctgttgg aacggcaata
      901 ataagggtgg tggttgcaaa gaaaaaacca aaccaaggat tcattcctga aacatacatg
      961 tgatgcgccc agacaataaa gcttaaagct ccaattataa ctatagccca aaccatcatg
     1021 cgataaccaa aaatattttt tcttgcatga
//
LOCUS       DTVV01000074            1446 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1220537, whole genome shotgun sequence.
ACCESSION   DTVV01000074 DTVV01000000
VERSION     DTVV01000074.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1220537"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            133..753
                     /locus_tag="EYO86_00895"
     CDS             133..753
                     /locus_tag="EYO86_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIC41626.1"
                     /translation="MFYEPKKGHPFSIDPYKSLIFPRPIGWISTISKKGIPNLAPYSY
                     FNAVADHPPQVMFSEEAPISYSRYKDSLTNILSTKEFVVNFATSKICKQMALSSIDAP
                     LDIDEFKFTKLKKRKSKKVKAPSVAESPVNLECKLLKTFKLKSNYKKYGTIVIGEVIG
                     IFINNKFIKKGRVDSAAMRYVARLGYSEYTTVTSKFKMHHPKWKNK"
     gene            750..1217
                     /locus_tag="EYO86_00900"
     CDS             750..1217
                     /locus_tag="EYO86_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIC41627.1"
                     /translation="MSDDQIKDILKNSKTIAMVGISSEKKGEDPKNLKRKPANVVMKY
                     MQDFGYKVIPVNPFAEGEIINGETVAASLENISESVDIVDIFRPSKETPNIANEAVKI
                     GSKVLWLQYGIQNEEAKKIAEKANMQYVSNRCIKQEYQRLFQKVNPVFPALKN"
     gene            1218..>1446
                     /locus_tag="EYO86_00905"
     CDS             1218..>1446
                     /locus_tag="EYO86_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017969585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIC41628.1"
                     /translation="MKKIFLLILFFQTSAFSQNLGKAYFAGGCFWCMEESFENEEGVL
                     EVISGYSGGITENPTYEEVTYGTTGHFETIEI"
BASE COUNT          571 a          191 c          245 g          439 t
ORIGIN      
        1 atggcgatgg atgccaaaga aggaatatct gcttttttag aaaaaagaaa accaaaatgg
       61 aaaaataaat gagtgatgat caaataaatt tatttgatat agtgtttgta attattttct
      121 ttgggaggat taatgtttta tgaaccaaaa aaaggacatc cattttccat tgatccatat
      181 aaatctctca tttttccaag acctatcggt tggatttcaa ccataagcaa aaaaggcata
      241 ccaaatttag cgccttattc atactttaat gctgtagctg accatccacc acaagttatg
      301 ttctctgaag aagcaccaat ttcatatagt aggtataaag attctttaac aaatattctt
      361 tcaactaaag aatttgttgt taattttgct acttcaaaaa tctgcaaaca gatggctctt
      421 tcatctatag atgcaccttt agatattgat gaatttaaat ttacaaaact taaaaaacgt
      481 aaatcaaaaa aagtaaaagc cccatcagta gctgaaagtc ctgttaatct agaatgtaaa
      541 ttattaaaaa ctttcaagtt aaaatctaac tataagaaat atggtacaat tgttattggt
      601 gaagttatcg gaatatttat taataataaa tttataaaaa aaggacgtgt agatagtgcg
      661 gctatgcgtt acgttgcacg attgggatat tctgaataca ctacagtcac ttccaaattt
      721 aagatgcacc atccaaaatg gaaaaataaa tgagcgatga tcaaataaaa gatattctca
      781 aaaattcaaa aacaattgca atggttggaa taagttcaga aaaaaagggc gaagatccaa
      841 agaatttgaa aagaaaacct gccaatgtag ttatgaaata tatgcaagat tttggctaca
      901 aagtaatacc agttaatcct tttgctgaag gagaaataat aaatggagaa acagtagctg
      961 ctagtttaga aaatatttca gaatcggtag atattgttga tatatttcga ccatcaaaag
     1021 aaactcctaa tattgcaaat gaggcagtta aaattggaag caaagtatta tggttacaat
     1081 acggaattca aaacgaagag gcaaaaaaaa ttgctgaaaa agccaacatg caatacgttt
     1141 caaacagatg tataaaacag gaatatcaga gattatttca aaaagtcaac ccagtatttc
     1201 cagctttaaa aaattaagtg aaaaaaattt ttttgttaat tttatttttt caaacaagtg
     1261 cttttagtca aaatttagga aaagcatatt ttgctggagg ttgtttttgg tgtatggagg
     1321 agtcttttga aaatgaagaa ggagtattag aagtaatttc tggttactct ggaggcataa
     1381 ctgaaaatcc gacatatgaa gaggttacat atgggacaac aggacatttt gaaactattg
     1441 agatta
//
LOCUS       DTVV01000075            2679 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1221729, whole genome shotgun sequence.
ACCESSION   DTVV01000075 DTVV01000000
VERSION     DTVV01000075.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1221729"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="EYO86_00910"
     CDS             complement(<1..1759)
                     /locus_tag="EYO86_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HIC41629.1"
                     /translation="MSEQFTGAEIVLKSLLDQGVEVIFGYPGGAVLPIYDTLFKQNSI
                     RHILVRQEGGAIHAAEGYARSSGKIGVVLVTSGPGVTNVVTGLTDAMMDSVPVVCITG
                     QVPQHLIGSDAFQECDTTGITRPCTKHNYLVKDVNKLSGVFHEAFAIANNGRPGPVLI
                     DIPKDVQFAKGKYLEPNSITIPKHRLFEPKVKGNEGQIYEASKLIANAKKPIFYIGGG
                     CINSGLKASNLVREFVKKTGAPATMTLMGLGVLPSSNKYSLGMLGMHGMYEANLAMHD
                     CDVMINIGARFDDRVTGRLDAFSPSSKKIHIDIDASSINKNIKVDIPIIGDIESIMNQ
                     FQDVWKKNKFSTNKDALEAWWKKIEEWKKIKCLDFEQKDEIIKPQQAVKRLYELTKGK
                     NTIITTEVGQHQMWAAQYYKFEEPNRWLTSGGLGTMGYGFPAAIGAQVANPEALVIDI
                     AGDASILMNMQEMSTAVQHRLPVKIFILNNEYMGMVRQWQELLHGGRYSESYTDSLPD
                     FVKLAESYRAKGLRAKKASELDKVITEMIETDNTVIADIWVNKEENCFPMIQSGSAHN
                     EMLLNKDQTQDKQASEKGKV"
     gene            complement(1816..2229)
                     /locus_tag="EYO86_00915"
     CDS             complement(1816..2229)
                     /locus_tag="EYO86_00915"
                     /inference="COORDINATES: protein motif:HMM:PF01715.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41630.1"
                     /translation="MVSPASLLVNGKKKQNNFFLNKPDYHIYLFTPSREKIYERVNVR
                     FKKMLNQGAIEEVRQLILLKLDKSLPIIRAHGVPEITDYLSNVSTLDDCISRGQQITR
                     NYVKRQLTWWRSCSLRIHQSFDEFPEKIDEKLLKI"
     gene            complement(2180..>2679)
                     /locus_tag="EYO86_00920"
     CDS             complement(2180..>2679)
                     /locus_tag="EYO86_00920"
                     /inference="COORDINATES: protein motif:HMM:PF01715.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIC41631.1"
                     /translation="LALRLAEKINGVIINADSMQVYSNLKILTARPSSADLKRISHQL
                     YGYVDGSIRYNVSKWCNDILKVIQKNQINNTPLIIVGGTGMYIDKLLNGLIDIPSIPE
                     LIKEKSENLVKKVGLGNFLKIVNDIDPVSLNNISANDTNRLKRIWEVYSFTGIPFSEW
                     KKKTK"
BASE COUNT          786 a          514 c          386 g          993 t
ORIGIN      
        1 aaactttccc tttctctgac gcctgtttat cttgtgtttg atctttattt aatagcattt
       61 cattgtgagc tgatccactt tgtatcattg gaaaacagtt ttcttcttta ttcacccata
      121 tgtctgcaat tactgtatta tcagtttcta tcatttctgt aattacctta tctaattcgc
      181 tggctttttt ggctcttaat ccctttgctc tataactttc cgctaattta acaaaatcag
      241 gcaagctatc agtatagctc tccgaatatc tcccaccatg taataattct tgccattgtc
      301 tcaccatgcc catgtattca ttatttaaaa taaatatttt aacaggcaat ctgtgttgta
      361 cggcagtact catttcttgc atgttcatca aaattgatgc atcacccgca atatcaataa
      421 ccaatgcttc aggattagca acttgtgcgc caatagctgc tggaaaacca taacccatgg
      481 tacctaagcc tcctgaagtt agccatctat ttggttcttc aaatttataa tactgtgcag
      541 cccacatctg atgttgacca acttctgtag taattattgt atttttacct tttgtgagtt
      601 catataatct tttaacagct tgttgaggct ttataatttc atctttttgt tcgaaatcaa
      661 ggcattttat ttttttccat tcttcaattt ttttccacca agcttctagg gcatctttgt
      721 tagttgaaaa tttatttttt ttccaaacat cttgaaattg attcattata ctttcaatat
      781 ctccaataat tggaatatca actttaatat ttttattaat gctgcttgca tcaatatcta
      841 tatgaatttt ttttgaacta ggagaaaaag catcgagtct acctgttact ctatcatcaa
      901 atcttgcgcc aatgttgatc atcacatcgc aatcatgcat tgctaaattt gcttcataca
      961 tgccatgcat tcccaacatc cctaatgaat atttattaga cgatggtaat acccctaagc
     1021 ccattagggt catggttgct ggtgctcctg ttttttttac aaactctcta acaagatttg
     1081 aggcttttaa tcctgagtta atgcaacccc ctccaatata aaaaataggt tttttagcat
     1141 tagctattaa cttggaagct tcatatattt gtccttcatt gccttttacc ttaggttcaa
     1201 acaaacgatg ttttggtatt gttatactat ttggttctaa atatttgcct ttggcaaatt
     1261 gtacatcttt tggtatatca atcaaaactg gtcctggtct tccattattt gcaatagcaa
     1321 aagcttcatg aaaaactcct gacagtttat ttacatcttt aactaaataa ttatgttttg
     1381 tacaaggtct ggtaattcca gtagtgtcac attcttgaaa agcatcactt cctatcaaat
     1441 gttggggtac ttggcctgta atacaaacaa ctggaacact atccatcatt gcatcggtta
     1501 atccagtgac tacatttgta actcctggac ctgatgtaac taaaacaacc ccaatttttc
     1561 ctgaggatcg ggcataacct tcagctgcgt ggattgcccc tccttcttga cggacaagaa
     1621 tatgtctaat ggaattctgc ttaaataggg tatcataaat tggtaaaact gcgccgcctg
     1681 gatagccaaa tattacttct accccttgat ctaataagga ttttaaaact atttcagcgc
     1741 cagtaaattg ttcggacatt tccaaatgct cataggctga ggataaaaca aaatcaataa
     1801 attagttcat tatattcata ttttgagcaa tttttcatct attttttcag gaaactcgtc
     1861 gaaagactga tgtatgcgaa gtgaacatga tctccaccaa gttaactgcc ttttaacgta
     1921 atttctggta atttgctgcc ctctactaat acaatcatct aatgttgaca cgttggaaag
     1981 ataatctgta atttctggaa ctccatgagc tcgtataata ggaagtgatt tatctaattt
     2041 taataaaata agttgcctaa cttcttcaat tgctccttga ttaagcattt ttttaaacct
     2101 cacattcacc ctttcatata ttttttctct ggagggagtg aataaatata tatgatagtc
     2161 aggtttgttt aaaaaaaaat tattttgttt tttttttcca ttcactaaaa gggatgccgg
     2221 tgaaactata gacttcccat atccgtttta aacgattggt atcatttgca gaaatgttat
     2281 ttaaggatac tggatctata tcatttacga tttttaaaaa attaccaagg ccaacttttt
     2341 tcaccaaatt ttctgatttt tcttttatta actctggaat tgaaggaatg tcaatcaatc
     2401 catttaaaag cttatcaata tacattcctg tacctccaac aataatcaaa ggagtgttgt
     2461 tgatttgatt cttttgaatc actttcaaaa tatcattgca ccatttcgaa acattatacc
     2521 ttattgaacc atcaacatat ccgtataatt gatgagatat tcgtttaagg tcagcagaag
     2581 atgggcgtgc tgttagaatt tttagatttg agtaaacttg catactatca gcgttaatga
     2641 ttactccatt aattttctct gctaaacgaa gggctaaag
//
LOCUS       DTVV01000076            1024 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1222087, whole genome shotgun sequence.
ACCESSION   DTVV01000076 DTVV01000000
VERSION     DTVV01000076.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1222087"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="EYO86_00925"
     CDS             <1..117
                     /locus_tag="EYO86_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIC41632.1"
                     /translation="KFPSYDVAMNWYHSEEYAPVRKIREDNSEGNLIIVEGK"
     gene            232..759
                     /gene="thpR"
                     /locus_tag="EYO86_00930"
     CDS             232..759
                     /gene="thpR"
                     /locus_tag="EYO86_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIC41633.1"
                     /translation="MMRCFIGIDLPIVAVKEIQKIQKKLEPNFTGRLTSSENLHLTLK
                     FLGEIENESINDAKKRLSSITHPPFELTLKDVGVFSQKFIKIIWVKVSDVSLQSLVDN
                     CLNDIFESENRFMGHITIARVKNLADKKSFLQLINVTTVNEVSFMVKDFYLKESILAS
                     DGPIYKDIVRYKLSS"
     gene            872..>1024
                     /locus_tag="EYO86_00935"
     CDS             872..>1024
                     /locus_tag="EYO86_00935"
                     /inference="COORDINATES: protein motif:HMM:PF10861.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HIC41634.1"
                     /translation="MYYGLGANLTFAMHFAFIVFVIFGALILFVSKKIAFIHIPSVIY
                     GAYTELS"
BASE COUNT          354 a          130 c          165 g          375 t
ORIGIN      
        1 aagtttccaa gttatgatgt tgccatgaac tggtatcatt cagaggaata cgcaccagta
       61 aggaaaatta gagaggataa ctctgaagga aacttgataa tagttgaagg caaataaccg
      121 aatgaacaga aagttattat ttatcaatat ctttagttta ggtttacagt ggaaaccact
      181 tagttaacta aaataactct cttaatcttt taggaaatgg tggtatgata aattatgcga
      241 tgttttattg gtattgattt acctattgtg gcagttaaag aaattcaaaa aatacaaaaa
      301 aaacttgagc ctaattttac tggaagatta acctcaagtg aaaatttgca tctaactcta
      361 aagtttttag gcgaaataga aaatgaatca ataaacgatg caaaaaaaag attgtcctcc
      421 atcacgcatc cgccatttga attgacctta aaggacgttg gggttttttc tcaaaagttt
      481 atcaaaatca tatgggtgaa ggtttctgat gtatccttgc aatcattggt tgataattgt
      541 ttgaatgata tatttgaatc agaaaataga tttatgggac atattacaat tgctcgtgtt
      601 aaaaatcttg ctgataaaaa atcatttctt caattaatta atgttactac agtaaatgag
      661 gtatcattta tggttaaaga tttttatttg aaagaatcta ttctagcctc agatgggcca
      721 atttataaag acattgttag atataagtta tcatcataaa ataacttaat attttcattt
      781 taccaaatac ttcattgttt catctttatt attcttttaa ttttttagaa aataattgac
      841 cttttaactg taaagtggtg gaataccgga aatgtactat ggtttaggcg caaatttaac
      901 atttgcaatg cactttgctt ttatagtttt tgttattttt ggagcgttga ttctctttgt
      961 atcaaaaaaa attgctttta ttcatattcc ttctgttatt tatggagcat atactgaatt
     1021 atct
//
LOCUS       DTVV01000077            2459 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1222717, whole genome shotgun sequence.
ACCESSION   DTVV01000077 DTVV01000000
VERSION     DTVV01000077.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1222717"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="EYO86_00940"
     CDS             complement(<1..621)
                     /locus_tag="EYO86_00940"
                     /inference="COORDINATES: protein motif:HMM:PF02274.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HIC41635.1"
                     /translation="MNNYGAQNMVACLKKVLMKRPQKFMSKVDLQKWNYTSPLDQDFI
                     NENYNEFYKIIKNSGAEIVDLKLINENAELCDSIFTHDPSLVLKEGAIILNMGKKLRR
                     KETEEHINFYNKEQIPIIGKIINNGSVEGGDCLWINNKTLLVGESDRTNKSGINQLSG
                     ILNKHNILLIPIKLPKYDNEKSCFHLMSLISMLDDDLAIGCMSLLPL"
     gene            complement(623..1918)
                     /locus_tag="EYO86_00945"
     CDS             complement(623..1918)
                     /locus_tag="EYO86_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIC41636.1"
                     /translation="MRQLPANHQSLLQSIALIDCNSFYASCERIFNPKLLGKPIVVLS
                     NNDGCIITRSAEAKALGIKMGEPYFKAKKIIEKNNVKVFSSNYSLYGDISQRVMEILL
                     GFSPEVEIYSIDEAFLNFKGFKNHELLTYCKHIRQTIKQWVGIPVSIGVGSTKTLSKI
                     ANHLAKKEADYEGICILKGDEKIEEALNRIEIGDVWGIGRRLSKFLRNYGVCTAKQFA
                     FLDRRWIRKNMGVVGEKIQLELCGVSCLDLELLPSPKKSCCVSRSFSRPIEKIEELQE
                     SIANYGSRVAEKIREEGLIAQSMSIFVLTNHFNKKEKQYSSSIKLQLDYPTSDSKLIV
                     KRAVEGIQRIYKESYRYKKAGIILYELHSSSSVRGLLDYDKPRTDSLMRSLDEINYRY
                     GSATLRLAAEGVRRSWHMRREKVSPCYTTSFDQLMIVKS"
     gene            complement(1899..2342)
                     /gene="umuD"
                     /locus_tag="EYO86_00950"
     CDS             complement(1899..2342)
                     /gene="umuD"
                     /locus_tag="EYO86_00950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HIC41637.1"
                     /translation="MSEYKNLDLFSIIKKTQINTPVFLDKVSAGFPSPATDYMENKLD
                     LNEYLIRHPAATFIVKASGSSMVNADIYSGDLLIVDRSVTPKSNNIVIASIFGDLTVK
                     KLKKKEKSFFLISANDEYPSIEVKEEMECFIWGVVTYIIHETTSS"
BASE COUNT          783 a          451 c          316 g          909 t
ORIGIN      
        1 caagggtaat agagacatac atcctatagc tagatcgtca tccaacatgg aaattaaaga
       61 cattaaatga aaacaagatt tttcattatc atattttggt aattttattg gtattaataa
      121 aatattatgt ttatttaaaa ttccagatag ttgattgatg ccagatttat tagttctatc
      181 actttcacca acaagtagtg tcttgttgtt tatccaaagg caatctccgc cttctactga
      241 accattattt ataattttcc ctataattgg aatttgttct ttattataaa agtttatatg
      301 ttcctctgtt tcttttcttc ttaatttttt acccatattc aatataatag caccttcttt
      361 gagaactaat gaaggatcgt gggtaaaaat ggaatcgcat aattctgcat tctcatttat
      421 taattttaaa tctactattt ccgctcctga atttttaatt attttataaa attcattata
      481 attttcattt ataaaatctt gatctagggg tgatgtataa ttccattttt gaagatcaac
      541 cttagacatg aacttttgtg gcctcttcat taaaactttt tttaaacacg caaccatatt
      601 ttgtgcacca taattattca tatcaacttt ttacaatcat tagttggtca aaactagtgg
      661 tataacaagg tgaaactttt tctcttcgca tatgccaact tctccttaca ccttctgccg
      721 caagtcttaa tgttgcactg ccataacgat aattaatttc atccaaagat ctcattaaag
      781 agtcagttct tggcttatca taatccagta aacctcgtac ggacgaagaa ctgtgcaatt
      841 catataaaat tattccggct tttttataac gataactctc cttgtatata cgttgaattc
      901 cttcaacggc tctttttaca atcaatttgc tgtcgcttgt tgggtaatca agctgtaact
      961 ttatagagct tgaatattgt ttttcttttt tgttaaaatg gttggttaaa acaaatatac
     1021 tcatagactg tgctattagg ccttcttccc ttattttttc tgctacacgc gagccataat
     1081 tggcaataga ctcttgcagc tcttcaattt tctcgattgg acggctaaaa gacctagaaa
     1141 cacagcaact ctttttagga ctaggcaata attctaaatc caagcaggaa acaccacaca
     1201 attctagttg gattttctct ccaacaactc ccatgttttt cctgatccat cgtcgatcaa
     1261 gaaacgcaaa ttgtttagcg gtgcacaccc cataatttct taaaaatttt gacaaacgtc
     1321 ttcctattcc ccatacatcg ccaatttcaa ttctattcaa agcttcttca attttttcat
     1381 caccctttaa aatacatatc ccttcataat ctgcttcttt tttggcaaga tgattggcta
     1441 tttttgaaag tgtttttgtt gagccaactc caatgctaac tggaatgccg acccattgct
     1501 taatagtttg tctaatatgc ttacaatacg tcaacaactc atgattttta aatcctttaa
     1561 aatttaaaaa agcctcatca atggagtata tttctacctc aggggaaaat cctaaaagaa
     1621 tttccataac tctttgtgat atatctccgt ataatgaata atttgatgaa aaaactttta
     1681 cgttattttt ttctataatt tttttcgcct taaaatacgg ctctcccatt ttaattccca
     1741 aggctttagc ttctgctgaa cgtgtgatga tgcaaccatc attgtttgat aaaacaacaa
     1801 ttggcttgcc caataactta gggttaaata tcctttcaca cgatgcataa aaagaattac
     1861 aatcaatcaa tgcaattgac tgtagtagtg actgatgatt agctggaagt tgtctcatga
     1921 attatataag ttactactcc ccaaataaaa cattccattt cttctttcac ttcaatacta
     1981 ggatattcat cattagctga tataagaaaa aaagattttt cctttttctt caattttttt
     2041 actgttaagt ctccaaaaat tgaagctata acaatattat tacttttagg cgtaacacta
     2101 cgatcaacaa tcaataagtc accagaataa atatccgcat ttaccatcga tgaaccacta
     2161 gctttgacaa taaaggtggc tgcaggatgt ctaattaaat attcatttaa atctaactta
     2221 ttttccatgt aatctgtagc tggtgatggg aacccagctg aaaccttatc cagaaatacc
     2281 ggtgtattta tctgcgtttt tttaatgata ctaaaaaggt caagattttt gtattcagac
     2341 atcgtattta aaaattaaaa taaattcatg aaaaataata cttttatcaa tttttataat
     2401 attttgttct acttttgttc tactttatta aaattataaa gaaagtcaat aaaaatgaa
//
LOCUS       DTVV01000078            1707 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1226193, whole genome shotgun sequence.
ACCESSION   DTVV01000078 DTVV01000000
VERSION     DTVV01000078.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1226193"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(7..789)
                     /locus_tag="EYO86_00955"
     CDS             complement(7..789)
                     /locus_tag="EYO86_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020056611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC41638.1"
                     /translation="MLEIKNIQKNFGGLVAVNNASMNVTKGSITGLIGPNGAGKTTLF
                     NIIAGNLHSDLGEVYLEKEKISDLKAHELFEKGVLRTFQIAHEFSTLTVLENLMMVPG
                     NQFGENLLYSWFKNSIVSKQEEEIRAKAIAVIDFLNLNHLTQEVAGNLSGGQKKLLEL
                     GRTMMVDAKLVLLDEVGAGVNRTLLNEISDAIIRLNKEKDYTFFVIEHDMDLIEKICE
                     PVIVMAEGSVLFQGSFEEVKSNEEVIEAYLGRGIKQNKALKS"
     gene            complement(789..>1707)
                     /gene="rodA"
                     /locus_tag="EYO86_00960"
     CDS             complement(789..>1707)
                     /gene="rodA"
                     /locus_tag="EYO86_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIC41639.1"
                     /translation="DIKFIFKYSYLLFFLCLILLFSVEIIGIFGKGAERWIRIFGISV
                     QPSEIIKVAIILVLAKYFHNLRFDTIGKIYHIIIPAFFILVPFVLVLLQPDLGTAISI
                     FLLGVMILFIAGIRIWKFVLGVIISLSSFPFLWGLIKPYQQKRFLSFLNPELDPLGQG
                     YQLIQSKIALGSGGLAGKGFLEGTQSYLEYIPEKQTDFIFTLIGEEFGFIGTMFLLFL
                     FGLLITICFYISIKSYHIFGRIICLGVACNIFIYVILNMAMVTGLLPVVGIPLPLISY
                     GGTAMLSVMVSFGLLLNVCIYYNVKKINQ"
BASE COUNT          621 a          324 c          206 g          556 t
ORIGIN      
        1 aaaatatcat gactttaaag ctttgttttg ttttattcct ctgcctaaat aagcctcgat
       61 aacttcctca tttgatttta cttcctcaaa tgatccttga aataaaacac ttccttctgc
      121 catcacaata acaggctcac agatcttctc tatcagatcc atatcatgct caattacaaa
      181 aaatgtgtaa tctttctctt tattaagacg gataattgca tcactaattt catttaataa
      241 tgttctattt acgcctgctc ctacctcatc caataaaact agtttagcat caaccatcat
      301 tgtcctaccc aattctaata attttttttg gcctccagat agattgcccg ccacttcctg
      361 agttaaatga ttgagattta aaaaatcaat cactgcaatt gcttttgccc taatttcttc
      421 ttcttgtttt gatacaatag aatttttaaa ccatgaataa agaagatttt ctccaaattg
      481 attccctgga accatcatta aattctctaa taccgttaat gtggaaaatt catgcgcaat
      541 ttgaaaagta cgcagaacac ctttttcaaa aagctcatgt gctttcaaat ctgatatttt
      601 ttctttttcc aaataaactt ctcctaaatc tgaatgtaga ttgccagcta taatattaaa
      661 taaagttgtt tttcctgctc catttggacc aattaaccct gtgattgatc ccttcgttac
      721 gttcatagac gcattgttaa ctgctacaag tcctccaaaa tttttttgta tatttttaat
      781 ttctaacatc attgattaat ttttttaacg ttatagtaaa tacaaacatt tagtaacagc
      841 ccaaaagaaa ccatgaccga tagcattgct gtgccaccat atgaaattaa aggcaaagga
      901 atgccaacca ccggcaataa accagtcacc attgccatat ttaatataac atatataaat
      961 atattacagg caactcccaa acatataatt cggccaaata tatgataaga tttaatactt
     1021 atataaaaac aaattgtaat aagtagccca aacaagaata aaagaaacat tgtgcctata
     1081 aatccaaatt cttcaccaat aagtgtaaaa atgaaatcag tttgcttctc tggaatatat
     1141 tctagataag attgtgttcc ttccaaaaaa cctttaccag ctaagcctcc tgagcccaaa
     1201 gcaatttttg attgaattaa ctgataccct tgccccaaag gatctaactc tggattaaga
     1261 aatgataaaa atcttttttg ttgataaggt ttaattagac cccaaagaaa aggaaaagag
     1321 ctaagggaaa taataactcc aagaacaaat ttccaaattc ttattccagc gataaacaag
     1381 atcataacac ccaaaagaaa aatacttatc gcagtgccaa gatcaggttg tagtaaaaca
     1441 agcacaaatg gaactaatat aaaaaatgcc ggaataatga tatgatatat ttttccaata
     1501 gtatcaaatc ttaaattatg aaaatatttt gcgagtacca gaataattgc aactttaatt
     1561 atttctgaag gttgaacact tataccgaat attcgtatcc atcgctcggc tccttttcca
     1621 aagattccaa ttatttcaac agaaaataaa agtattaaac ataaaaagaa taataggtat
     1681 gagtatttaa aaataaattt gatatct
//
LOCUS       DTVV01000079            1371 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1227001, whole genome shotgun sequence.
ACCESSION   DTVV01000079 DTVV01000000
VERSION     DTVV01000079.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1227001"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1371)
                     /locus_tag="EYO86_00965"
     CDS             complement(<1..>1371)
                     /locus_tag="EYO86_00965"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIC41640.1"
                     /translation="LNILSWQPNVLSREFWQFVWVEYPIIYYLFSITTISYLWYKLLT
                     KIIQKQKQQSGSFFRSISFFIIGFLLIGTFIRGGWQQRPIDWGHAIFSDNLFANQTAL
                     NPLFNLGRSVIQLNSEKNIAKLVKYMDNSSALLITQKTIKTSGEIFTDSSSFKREIIK
                     SHVIKPNIVLVVLESFLGKYCGSANSGGIDVTPNLNRMADNGINCTRTIASGKRSAYG
                     LSTILCSWPPLPGFPLISQVESQQEVETVASLLKDIGYSTWFMYGGDADFDNMKGFVL
                     ANGFDHLIEQNDFPKNTPGTMWGVFDEYLFDYAESILDTSQVPVLLTMFTTTNHQPWK
                     IPKQMEKVIPHFLNKNKVIKKVLRTMAYTDYIIGEFIEDNRSEAWFQNTIFVFISDHG
                     INEYSGMYEDPRNAHIPFIIYSPAIITKSKKINEVTSQIDVLPTLLHLIGYPKPFDLM
                     GTNILSD"
BASE COUNT          439 a          230 c          239 g          463 t
ORIGIN      
        1 atcagagaga atattagtac ccatcaaatc aaatggtttg gggtacccaa tcaaatgaag
       61 taatgtgggt aggacatcta tttgactggt aacttcattt atttttttag actttgtaat
      121 aattgcaggt gaataaatta taaagggaat atgggcatta cgtgggtcct catacatacc
      181 gctgtattca ttaataccat gatctgagat aaaaacaaaa atggtgtttt gaaaccaggc
      241 ttctgatcga ttgtcttcta taaactcacc aatgatgtaa tctgtatatg ccatggtgcg
      301 cagtactttt ttgattactt tatttttatt tagaaaatgg gggattactt tctccatttg
      361 tttaggtatt ttccatggtt gatgattagt tgttgtaaac attgtaagta aaacagggac
      421 ttgcgaagta tcaagaatcg attctgcata atcaaataaa tattcatcaa aaacacccca
      481 catagttccc ggagtatttt ttggaaagtc attttgttca attaaatggt caaatccatt
      541 tgctaataca aaacctttca tattgtcaaa atctgcatct cctccgtaca taaaccaggt
      601 ggaataccca atgtccttta ataatgaagc gacagtttct acctcttgtt gtgattcgac
      661 ttgagatatg agaggaaatc ccggtaatgg aggccatgaa catagaattg tacttagccc
      721 ataggctgac cgcttccctg atgctattgt gcgggtacaa tttattccat tatctgccat
      781 acgatttaag ttgggggtta catctattcc tccagaattt gcagaaccac agtattttcc
      841 aaggaatgat tctaaaacaa ccagaactat atttggtttt atcacatgtg atttaattat
      901 ttctcgttta aaggaagaag aatcagtaaa tatttcccct gaggttttta tagttttttg
      961 ggttattaat aatgctgagc tattatccat atatttaacc aacttcgcga tatttttttc
     1021 tgaattcaat tgaataaccg accgcccaag gttaaaaagc ggatttaatg ctgtctgatt
     1081 tgcaaataga ttatcactga atattgcatg tccccaatcg ataggtcttt gttgccagcc
     1141 gcctcttatg aaagtcccga ttaataaaaa accaattatg aaaaatgaaa tacttctgaa
     1201 aaatgaaccg gattgctgtt tttgtttttg gatgatttta gttaacaatt tataccatag
     1261 ataactgata gtagtaatgc taaatagata ataaataatt ggatattcaa cccaaacaaa
     1321 ttgccaaaac tcccggctca gtacatttgg ttgccaggaa agaatattta g
//
LOCUS       DTVV01000080            1230 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1231810, whole genome shotgun sequence.
ACCESSION   DTVV01000080 DTVV01000000
VERSION     DTVV01000080.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1231810"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="EYO86_00970"
     CDS             <1..541
                     /locus_tag="EYO86_00970"
                     /inference="COORDINATES: protein motif:HMM:PF08450.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIC41641.1"
                     /translation="NDVCVKSDNTIWFTDPPYGILSDYEGYPGEQEYGGCYVFCFNTK
                     KNILKVITTNLDRPNGITFSHDEKKLYIADTGENIKCMYVFDVEGDLISNQKLVYDFK
                     PFFSDGFRCDKDGNIWTSAGKAIKCFNPQNELIGQLILPELVSNLEFGGKEGNTLYVT
                     ATSSLYAMELNQIGAKFPI"
     gene            541..876
                     /locus_tag="EYO86_00975"
     CDS             541..876
                     /locus_tag="EYO86_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIC41642.1"
                     /translation="MATNLNNLKCTPCSGKTPKLSYDEISINFKKLSNWSINDEKEML
                     FKKFIFKTFKAALHFTNSVGDLAEKEGHHPDISLGWGYCLIMIHTHAIKGLSINDFVL
                     ASKIDLIDS"
     gene            931..>1230
                     /locus_tag="EYO86_00980"
     CDS             931..>1230
                     /locus_tag="EYO86_00980"
                     /inference="COORDINATES: protein motif:HMM:PF13580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIC41643.1"
                     /translation="MDYKKYFFHYSDSISELLKNVDTNLINDSVNLIANTKKNKNKIY
                     IVGNGGSSSIASHVSVDFAKVAKVNCSTFNNANLITCFANDYKYENWVVEAIKAYS"
BASE COUNT          453 a          136 c          202 g          439 t
ORIGIN      
        1 caatgatgtt tgtgtaaaat cagacaatac aatttggttt actgatcctc catacggaat
       61 tttatcagat tatgaaggtt atcctggaga acaagaatat gggggatgct acgttttctg
      121 ttttaataca aaaaaaaata tacttaaagt tattacaact aatcttgata ggcctaatgg
      181 aattactttt tctcatgatg aaaaaaaatt atatattgct gatacaggag aaaatattaa
      241 atgcatgtac gtttttgatg tagagggaga tttaatatca aatcaaaaat tagtttatga
      301 tttcaaacct tttttttctg atggcttcag atgtgataaa gatggtaaca tttggacaag
      361 tgctggaaaa gcaatcaagt gttttaatcc tcaaaatgag ttaattggtc aactgattct
      421 cccagagtta gtttcaaatt tggaatttgg aggcaaggaa ggtaatactt tatatgtaac
      481 agcaactagt tcattgtatg caatggagtt aaatcaaata ggagctaaat ttccaattta
      541 atggcaacaa atttaaataa tttaaaatgc accccttgtt cgggcaaaac accgaaacta
      601 agttatgatg aaatatctat caattttaaa aaattgtcca attggagtat taatgatgaa
      661 aaagaaatgc tttttaaaaa atttatattt aagacattta aagcggcact tcattttact
      721 aattctgtag gtgatttggc agaaaaagaa ggtcatcatc cagatatctc tttgggttgg
      781 ggttattgct taataatgat acatacgcat gcaataaaag ggctttcaat taatgatttt
      841 gttcttgctt ctaaaattga tttaattgat tcttgaatat atgagaaatt cttgttttat
      901 ttttttatat aaactgtcat aattgcttgt atggattata aaaaatattt ttttcattat
      961 tcagattcaa tatctgaatt attaaaaaat gtagatacaa atttaattaa tgattctgtt
     1021 aatttaatag ctaatacaaa aaaaaataaa aataaaattt atatagtggg aaatggagga
     1081 agttcatcta tagctagtca tgtaagtgtt gattttgcaa aagtagctaa ggtaaattgt
     1141 tcaacattta acaatgctaa tttgataact tgttttgcca atgattataa atatgagaat
     1201 tgggtagttg aagctattaa agcatattca
//
LOCUS       DTVV01000081            1212 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1233000, whole genome shotgun sequence.
ACCESSION   DTVV01000081 DTVV01000000
VERSION     DTVV01000081.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1233000"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /gene="hrcA"
                     /locus_tag="EYO86_00985"
     CDS             <1..198
                     /gene="hrcA"
                     /locus_tag="EYO86_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037882.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIC41644.1"
                     /translation="VQIYIGAQNFLFNHSGLSMVMAPYRDKDQKIIGAIGVVGPTRLN
                     YAKIVPLVDYTSKIIGKVLCK"
     gene            195..824
                     /gene="grpE"
                     /locus_tag="EYO86_00990"
     CDS             195..824
                     /gene="grpE"
                     /locus_tag="EYO86_00990"
                     /inference="COORDINATES: protein motif:HMM:PRK14140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIC41645.1"
                     /translation="MSKKNKAKLNKEDELEKNNSDIVNSESENLEQNSTVANDTDADN
                     EKQNELEENLRDEIEQLRDEKLRLLADMENLRKRSDRDRMDSIRYGNINFARDILSLG
                     DNLSRALDAIPKDAEKTETITNLINGLRMVQREFTSILEKHGIKKIEALNQRFDHNFH
                     QAMMEIESEEVEEGIVIQEIQSGYNMHDRLLRPSMVGVAKKPNKDEKKK"
     gene            929..>1212
                     /gene="dnaK"
                     /locus_tag="EYO86_00995"
     CDS             929..>1212
                     /gene="dnaK"
                     /locus_tag="EYO86_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450925.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIC41646.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGKETKVIENSEGTRTTPSMVAFTE
                     SKEKLVGQSAKRQAVTNPENTLFAIKRLIGRGFADDVVKKDSKLVPFKIT"
BASE COUNT          479 a          163 c          230 g          340 t
ORIGIN      
        1 gtgcaaattt atattggtgc tcaaaatttt ttatttaatc actctggtct ttctatggta
       61 atggccccat atagggataa ggatcaaaaa atcattggtg caattggtgt ggttggtcct
      121 accagattaa attatgctaa aatagttcca ctggtagatt atacatcaaa aattatagga
      181 aaggtattgt gtaaatgagt aaaaaaaata aggctaaatt aaacaaagaa gatgaattag
      241 agaaaaataa ttctgatatt gtaaacagtg aaagtgaaaa tttggaacaa aatagcacgg
      301 ttgctaatga caccgacgca gataatgaaa agcaaaatga attagaagaa aatcttcgtg
      361 atgaaattga gcaactaaga gatgaaaaat tacgattgct tgcagatatg gaaaatttgc
      421 gcaaacgttc tgatagagat aggatggatt ctatcagata tggaaatata aactttgcac
      481 gtgatatttt atcccttgga gacaacttat cacgagcctt agatgccata ccaaaagatg
      541 cagaaaagac tgaaacaatc accaatttaa ttaatggttt acgaatggtt caaagagaat
      601 ttacctcaat tttagagaaa catggaatta aaaaaataga ggctctcaat caaagatttg
      661 accataactt tcatcaagcg atgatggaga tagaaagcga agaggttgaa gagggaattg
      721 taatacagga aatacaaagt ggctataaca tgcacgatag attgctccgt ccgtctatgg
      781 taggcgttgc aaaaaaaccc aataaagatg agaaaaaaaa gtaatattaa ctagtttttt
      841 aaccttatcc ttgaaatatt aatcttattt accatattca aggtaacaat tttaacaatt
      901 tgatacaatt taagaaacgg attttagtat ggcaaaagta ataggcattg atttaggcac
      961 taccaattct tgtgtagctg tcatggaagg aaaagaaact aaagtaatag aaaattcaga
     1021 gggtacaaga acaacacctt caatggttgc ttttaccgaa tcaaaagaaa aattggttgg
     1081 acaatcggca aaacgtcaag ctgtaaccaa tcctgaaaat actttgtttg ccattaaacg
     1141 tttgattgga agaggttttg cagatgatgt tgtaaaaaaa gatagcaaac ttgttccatt
     1201 caaaattact aa
//
LOCUS       DTVV01000082            1912 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1235541, whole genome shotgun sequence.
ACCESSION   DTVV01000082 DTVV01000000
VERSION     DTVV01000082.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1235541"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="EYO86_01000"
     CDS             complement(<1..585)
                     /locus_tag="EYO86_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIC41647.1"
                     /translation="MSDKLITSIYEITKNYYDPISNIPLNENNSNLSIICKDGHANIS
                     LNINQQDQEKYTDLSKNLKNALSNINALLSVNIIFTSEKTTDPASKKHYRFQISAKNI
                     IAIASGKGGVGKSTFAVNFAVALKKIGYQVGILDADIYGPSVPRMMGITGRPQTNDNK
                     KLIPLESYGIKCMSIGFLIGVDTPTIWRGPMVMKA"
     gene            677..1768
                     /gene="hflK"
                     /locus_tag="EYO86_01005"
     CDS             677..1768
                     /gene="hflK"
                     /locus_tag="EYO86_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIC41648.1"
                     /translation="MNVNKNNNDNNPWDSGGNNPWGGGSSGNKDLEDSIKKAKEKFGK
                     FKIGGPRNISILIIVALIIWLATGFYRVEPDEQGIELLFGKWNQVTTEPGLHYFFPTP
                     FGKTMTPKVESIRKINVGYRSASELGFSSNSSDRNVLEESLMLTGDQNIVDVNFTVLY
                     KIKDAGKFLFKLRNPETTVKDMSESVMREVVGQRDLEFLLTGGRQEVEQVVRFDLQDI
                     LDEYESGILVQSIQLQSVNPPALVIDAFDEVQRARQDKEKLVNEANSYMNKIVPNARG
                     DAAKLVQEATAYKEQVIKQAEGVAQNFIDVYNSYKETKYVTRQRIYLETLTEVLEGPN
                     KIILDSTGEGQGVVPYLPINELIKNKVNN"
     gene            1768..>1912
                     /locus_tag="EYO86_01010"
     CDS             1768..>1912
                     /locus_tag="EYO86_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIC41649.1"
                     /translation="MRFTVRKFIIVLILFIGLLTFTGAFFVVQEWKQVIVLQFGEPRQ
                     VITE"
BASE COUNT          645 a          277 c          361 g          629 t
ORIGIN      
        1 agctttcatt accattggtc ctctccaaat tgtcggggta tcaacaccta tcaaaaatcc
       61 aattgacata catttgatgc cgtaactttc taaaggtata agttttttat tatcattggt
      121 ttgaggcctg cctgtaatac ccatcatacg gggaacactc ggtccataga tgtcagcgtc
      181 tagaatgcca acttgataac ctattttttt taaggctaca gcaaagttaa cagcaaatgt
      241 tgacttccca actccacctt ttccgctggc tatggcgata atatttttag cacttatctg
      301 aaaacgataa tgttttttgg atgctggatc agtagttttt tctgaggtaa aaataatatt
      361 tacggataat aaagcattaa tgttgctaag agcatttttt aagtttttgc ttaaatcggt
      421 atatttttcc tgatcttgtt gattaatatt gagtgaaatg ttggcatggc catctttgca
      481 aattatgctc aaatttgaat tattttcatt taaaggaata ttcgatattg gatcgtaata
      541 attttttgtt atttcataga tacttgttat taatttgtcc gacatacttg aattttccaa
      601 ttttacttac aatatagcta gataaaacta aatataatgg tattagatct caatatacta
      661 atatcaagaa aatctcatga atgtaaataa aaataataac gataataatc cttgggattc
      721 tggaggcaat aatccgtggg gcggaggatc gtctggtaat aaggatttgg aagattctat
      781 taaaaaagct aaagaaaaat ttggtaaatt taaaatcggt ggacctagaa acatttcaat
      841 actaattata gtagctctta taatatggtt agcaaccggt ttctatcgtg tagagcctga
      901 tgaacaaggt atagagttgt tattcggaaa atggaatcaa gtgactacag aaccaggtct
      961 tcattatttc tttcctactc cgtttggaaa gacaatgact ccaaaagttg aaagtatcag
     1021 aaaaataaat gttggataca gatcggcttc cgaattgggg tttagttcta atagtagcga
     1081 cagaaacgtt ttagaagaaa gcttgatgtt aacaggtgat caaaacatag tcgatgttaa
     1141 ctttactgtt ttgtataaaa ttaaagatgc aggaaagttt ttattcaaac ttcgtaatcc
     1201 ggaaacaact gtaaaggata tgtctgaaag tgtaatgagg gaagtagttg ggcaaagaga
     1261 tctcgaattt ttactaactg gtggccgtca agaagtcgaa caagttgtca gatttgattt
     1321 acaagacatc cttgatgagt atgaaagtgg gatattggtt cagtcgatac aacttcaaag
     1381 tgttaacccg cccgctctgg tcattgatgc atttgatgaa gttcaaagag cacgtcaaga
     1441 taaagaaaaa ctggttaatg aagccaattc ttatatgaat aaaattgtgc caaatgccag
     1501 aggtgatgca gctaaacttg ttcaagaagc aacagcatac aaagagcagg ttatcaagca
     1561 agccgaaggt gttgctcaaa atttcattga tgtttataac agctataaag aaacaaaata
     1621 tgttactcga caaagaattt atttagaaac tttgacagaa gttttagaag gtccaaataa
     1681 gattatttta gatagtaccg gtgaaggcca gggtgttgtg ccttatttac ccataaatga
     1741 attaattaaa aacaaggtta ataactaatg agatttacag ttcgaaaatt tattattgtt
     1801 ttaattttgt tcattggatt acttacattt accggtgctt tttttgttgt tcaagaatgg
     1861 aagcaagtaa ttgttttaca gtttggagaa cctcgtcaag taataactga ac
//
LOCUS       DTVV01000083            1233 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1236530, whole genome shotgun sequence.
ACCESSION   DTVV01000083 DTVV01000000
VERSION     DTVV01000083.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1236530"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            8..832
                     /locus_tag="EYO86_01015"
     CDS             8..832
                     /locus_tag="EYO86_01015"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIC41650.1"
                     /translation="MKKVKINNFEISNSNPFCLIAGPCLIESRDHTMKMAELIYEICQ
                     ETRINFIFKSSFDKANRTSVQSTRGVGLFKALEIFNEIKSNINCPILTDIHNETQCNE
                     ISGSDIVDIIQIPAFLCRQTDLLIAAGKSKKIINVKKGQFLSPHDMPNIIEKIHTTNN
                     QQILLTERGTSFGYNNLVSDFRSIEIMKGFEYPVIFDATHSVQEPGGLGMKSGGKREY
                     IPVLSKAAIAVGVAGIFIETHDDPDNAPSDGPNMLNVKDLKSLILKLIELDNVSKS"
     gene            857..>1233
                     /gene="eno"
                     /locus_tag="EYO86_01020"
     CDS             857..>1233
                     /gene="eno"
                     /locus_tag="EYO86_01020"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867129.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIC41651.1"
                     /translation="MAIIKNIKAREILDSRGNPTVECDIYLDDNSFGRSSVPSGASTG
                     MHEAIELRDNDKGRYGGKGVLKAISNILEIVAPKIINQSFDNFYDFDENLLSIDGTKN
                     KSNLGANATLALSLAYAKALAFQ"
BASE COUNT          463 a          164 c          215 g          391 t
ORIGIN      
        1 aaaaaaaatg aaaaaagtta aaataaataa ttttgaaatt tcaaactcaa accctttttg
       61 tcttattgca ggcccttgtt taattgagag cagagatcat actatgaaga tggcagagct
      121 aatttatgaa atttgccaag agacaagaat taattttatt tttaaatcca gttttgataa
      181 agctaataga acaagtgttc aaagtacaag gggagttggt ttattcaaag ctttagaaat
      241 ttttaatgaa attaaatcaa atattaattg tccaattcta actgatattc ataatgaaac
      301 ccaatgtaat gaaatatctg gaagtgatat tgtagatata attcaaattc ctgcattttt
      361 atgtcgtcaa acagatttat taattgctgc tggtaaatca aaaaaaataa ttaatgtcaa
      421 aaaaggccaa tttctttcac ctcatgatat gcctaatata atagaaaaaa ttcatactac
      481 taataaccaa cagatacttt taacagaaag aggaacttct tttgggtata ataatttagt
      541 aagtgatttt aggtcaattg aaataatgaa aggtttcgaa tatccagtaa tttttgatgc
      601 aacacattct gtacaagaac caggaggatt aggcatgaaa agtggtggta aacgcgagta
      661 tataccggtt ctttctaaag cggccatagc tgtaggtgtt gctggcatct ttatagaaac
      721 gcatgatgac cctgataatg ctccaagtga tggaccaaat atgctcaatg ttaaagactt
      781 aaaatcctta attttaaaat taatagaatt agataacgtg tctaaatctt agtgatttaa
      841 aagatagtga agagaaatgg caattatcaa gaatattaaa gcaagggaaa ttttggatag
      901 cagaggaaac ccaactgttg aatgtgatat atatctcgat gacaattctt ttggaagatc
      961 ctccgttcct tctggtgcct caacaggtat gcatgaagca atcgaattaa gggataatga
     1021 caaaggtaga tatggaggta agggggtttt aaaagctata tctaatatct tggagattgt
     1081 agcccctaaa ataataaatc agtcttttga taatttttat gattttgatg aaaacttact
     1141 atctattgat ggaactaaaa ataaatcaaa tttaggggct aatgcaactc tagctcttag
     1201 tctagcttat gctaaagcgc tagcatttca aaa
//
LOCUS       DTVV01000084            1323 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1239142, whole genome shotgun sequence.
ACCESSION   DTVV01000084 DTVV01000000
VERSION     DTVV01000084.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1239142"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /gene="leuS"
                     /locus_tag="EYO86_01025"
     CDS             <1..1147
                     /gene="leuS"
                     /locus_tag="EYO86_01025"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:PF09334.9"
                     /note="leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA
                     synthetase; charges leucine by linking carboxyl group to
                     alpha-phosphate of ATP and then transfers
                     aminoacyl-adenylate to its tRNA; due to the large number
                     of codons that tRNA(Leu) recognizes, the leucyl-tRNA
                     synthetase does not recognize the anticodon loop of the
                     tRNA, but instead recognition is dependent on a conserved
                     discriminator base A37 and a long arm; an editing domain
                     hydrolyzes misformed products; in Methanothermobacter
                     thermautotrophicus this enzyme associates with prolyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIC41652.1"
                     /translation="GNALQKLDKWKETICPETGMKVTRETDTFDTFFESSWYFLRYCN
                     PRHSKPFDKEDIDYWLPVDQYIGGIEHAILHLLYSRFFVKALRDIKHINIDEPFKGLF
                     TQGMVTHQTFKNKKDEWLGPDEIIKKDYNYFDLENNVVDVGKIEKMSKSKKNVVDPTS
                     IINLYGADTARWFMLSDSPPDRDLEWTDSGIAGSYKFINKIWNLTKEVLNDSYNFSKS
                     SNDNLLYAKLDETIENVTNNINAFQYNKSVANIYELINFLQKQLNNNAVSKNCVLETL
                     KKLSLLLQPFIPHLSEELWSHLVGGDMAICQSWPIKIGMSKKSSYGIAIQVNGKTKDV
                     ILLDHTPEEKALFDILKNNNKIQKIIKNKKIIRTIYVPNRILNILIK"
BASE COUNT          519 a          180 c          216 g          408 t
ORIGIN      
        1 gggcaatgcg ctacaaaaac tagataaatg gaaagaaaca atatgccccg aaactggaat
       61 gaaggttaca agagaaacag acacttttga tacattcttt gaatcgtcat ggtatttttt
      121 aaggtattgt aatcctcgac atagcaaacc ttttgataaa gaagatattg attattggct
      181 cccagttgat cagtatatag gtggaattga acacgctata ctgcaccttt tgtattctag
      241 attctttgtt aaagctctta gagatattaa acacattaat atagatgagc catttaaagg
      301 attgtttaca caaggaatgg ttactcacca aacatttaaa aataaaaaag acgaatggtt
      361 gggtcccgat gaaataataa aaaaagatta caattatttt gatttagaaa acaatgttgt
      421 tgatgttggt aaaattgaaa aaatgagtaa atcaaaaaaa aatgttgttg acccaacatc
      481 aataattaat ctttatggag ctgatactgc taggtggttt atgttatctg atagtccacc
      541 agacagggat ctggagtgga cggacagtgg aatagctgga tcttataaat ttattaacaa
      601 aatttggaat ttaactaaag aagttttaaa tgactcatac aatttttcta aatcttcaaa
      661 cgataattta ctttatgcaa agctagatga aacgattgaa aatgtaacca ataacattaa
      721 tgcttttcag tataacaaat ctgtggcaaa tatttatgaa cttataaatt ttttacaaaa
      781 acaacttaat aacaacgcag tatctaaaaa ttgtgttttg gaaactctaa aaaagttatc
      841 tttattgctc caacctttta ttcctcattt aagtgaagag ctgtggagtc atctggttgg
      901 gggtgatatg gcaatatgtc aatcttggcc aataaaaatt ggcatgagca aaaaaagctc
      961 gtatggaata gctatacaag ttaatggtaa aacaaaggat gttattttgc tggatcacac
     1021 acctgaagaa aaagcattgt ttgatatatt aaaaaacaac aacaaaatac aaaaaatcat
     1081 taaaaacaaa aaaataatca gaactatcta tgttccaaat agaatactaa acatactaat
     1141 taaataaatg aatataaaaa tatttttaat ggtccttgta accttaatag ttagctgtca
     1201 aggctttgat tttgtatatg ataaatcctc cacgataaag ttgttggaaa acaataccca
     1261 agtcataata agcggtgatg atattagtat aataaaatcc cagctaaaca attttgttgg
     1321 aac
//
LOCUS       DTVV01000085            2353 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1245078, whole genome shotgun sequence.
ACCESSION   DTVV01000085 DTVV01000000
VERSION     DTVV01000085.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1245078"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="EYO86_01030"
     CDS             <1..698
                     /locus_tag="EYO86_01030"
                     /inference="COORDINATES: protein motif:HMM:PF00691.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41653.1"
                     /translation="EEKTTTQEESTASIKEMKMRSTETLKQVAFLTEEIGALKNEIIL
                     LNAALEYSEQTLLTKELKIEVLGERLNKALTSKVFELQKYRSEFFGKLQLILGDRKDI
                     KIVGDRFIFESELLFNSASANLQSAGMEKLKQIGLTLMDTTQDIPADIDWIIQVEGHT
                     DKRSIKTVQYPSNWELSTARANSVLKLLLDLGFAPHRLSAAGYGEFYPLSDGDSDEDL
                     QQNRRIELKLTSR"
     gene            756..1256
                     /locus_tag="EYO86_01035"
     CDS             756..1256
                     /locus_tag="EYO86_01035"
                     /inference="COORDINATES: protein motif:HMM:PF13599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIC41654.1"
                     /translation="MKTFVFILIFFFPMKIFAACSDQPANEVDWTNCNFVESLELSGV
                     SLAGAQMSGVNLALANLEKSQINNANMAYGNFIFANFSNSNLFLSNLQYANCNNSNFD
                     NSNLAKVNFEGANLFSSSFKGANLFEVNLLGANITGAIFDEANLSGAIWVDGKTCALG
                     SVGVCN"
     gene            1314..2237
                     /locus_tag="EYO86_01040"
     CDS             1314..2237
                     /locus_tag="EYO86_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC41655.1"
                     /translation="MNIGHRSQFGSAYFSAFVLVVFSGLLWSFGVVTVRYMVDGQDYV
                     FQYLFYRGISIAVILIFYLFIREGLSFYENFFKVGISGFLGGIFLSTAFIGFIFSITM
                     TTAAVTLFMLAAMPFIAAIVGYFVLGEILRPTTLVSMIIAFIGVCIMILSDSILGSAI
                     GALIGLISATGFALYTVTIRWKPETPKFTTVVLAGIFCTIFSFCILGFSFESFALMPT
                     RNSYLSLLHGSLVASGLILYNLGAKYLPSAELALLSLMEVVGGVLWVWMPVFGINEVP
                     SATVIIGGLIITFAVLLHGFGARRKREPVTL"
BASE COUNT          683 a          381 c          460 g          829 t
ORIGIN      
        1 aagaagaaaa aacaacaacc caggaagaat caactgcgtc tatcaaagaa atgaaaatga
       61 gatcaactga aacattaaaa caagtagctt ttttaacgga agaaataggg gcattaaaaa
      121 atgaaattat attacttaat gcggcacttg aatatagtga acaaactctt ctaacaaagg
      181 agctaaaaat tgaagtctta ggggaaagat taaacaaagc cttaacgtcc aaagtatttg
      241 agctacaaaa atatcgatca gagttctttg gaaaattaca attaatttta ggtgatcgca
      301 aagatataaa aattgtaggt gatcgtttta tttttgaatc tgaattgctg tttaactcgg
      361 cttctgccaa tcttcagtca gcaggtatgg aaaaattaaa acaaattggc ttgacgttaa
      421 tggatacaac gcaagatatt ccagctgaca ttgattggat tattcaagtt gaaggccata
      481 cagataaaag atcaataaag acggttcaat atccatcaaa ttgggagtta tcgacagcaa
      541 gagctaattc tgttttaaaa ttgctacttg atttgggatt tgcaccccat agattatctg
      601 ccgcgggtta tggggagttt tatccattat ccgatggtga ttccgatgag gatttacaac
      661 aaaacagacg aattgaatta aaattgacct ctcgttaaaa ctgtaatacg tcataaatag
      721 aacataaata gatattagta ctttgcacaa cacttatgaa aacctttgta tttatattaa
      781 tctttttttt tcccatgaaa atatttgctg catgcagcga tcagcctgcc aatgaggtgg
      841 attggacaaa ctgtaatttt gttgaaagct tagagttatc cggagtctct ttggctggcg
      901 cgcaaatgtc aggtgtgaat ttagctttag ctaatttgga aaaatcccaa attaacaatg
      961 ccaatatggc gtacggtaat ttcatttttg ctaactttag taattccaac ttatttttat
     1021 caaatttaca gtacgctaat tgcaataatt caaattttga taattcaaat cttgccaaag
     1081 taaattttga gggtgcaaat cttttctcct cttctttcaa aggagccaat ttgtttgaag
     1141 tgaatttgtt aggggcaaat attacgggcg ctatttttga cgaggcgaat ttgtctggag
     1201 ctatttgggt tgatggcaaa acctgcgcgc tgggctctgt gggagtttgt aattaattag
     1261 caaaatattc aattttatat ttctgtaaaa tatgtaatgt cgcagatcaa tttatgaata
     1321 ttggtcatcg ctcccaattt ggcagtgctt atttttctgc gtttgtttta gttgttttct
     1381 ccggtttgtt atggagtttt ggagtcgtta ctgtacgtta catggttgat ggtcaagatt
     1441 atgtatttca gtacttgttt taccggggta tttcaattgc agttatttta attttttatc
     1501 tgtttattag agaaggcttg tcattttatg aaaatttttt taaagttggt atttctggat
     1561 ttttaggagg aattttttta agtaccgctt ttatcggctt cattttctct attacaatga
     1621 caacagctgc tgttacgtta tttatgttgg cagccatgcc ttttatagcg gctattgttg
     1681 gctactttgt tctaggagag atacttcgac ccacaactct tgtatccatg attatcgctt
     1741 ttattggtgt atgcataatg atacttagcg actccatatt aggaagcgca attggcgcct
     1801 tgattggatt gatatctgca actggttttg cgctttacac ggtcaccatt cgctggaagc
     1861 ccgaaacgcc aaaatttaca acggtcgtgt tagctggaat tttctgcact attttttctt
     1921 tttgtattct tggtttcagc tttgaatctt ttgcgttaat gccaacacgt aattcctatc
     1981 ttagtttatt acacggatcc cttgttgcct caggattaat tttatacaac cttggtgcga
     2041 aatacctgcc gtccgctgaa ctagctcttc tttcattgat ggaagttgtt gggggagtgc
     2101 tctgggtatg gatgcctgta tttggcatta acgaggtacc aagtgctaca gtcattatcg
     2161 ggggtctcat tataacgttt gctgtcctgc tgcatggttt cggcgctaga cgaaaaaggg
     2221 aacctgtaac actttaaatt ttacattttt tttgttttcc attaaatacc aagttcttaa
     2281 caaaattttg aatgaaaaaa attattctaa tatccatcgg ttggctttgt gttggcttgg
     2341 cctttatagg cac
//
LOCUS       DTVV01000086            2204 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1245611, whole genome shotgun sequence.
ACCESSION   DTVV01000086 DTVV01000000
VERSION     DTVV01000086.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1245611"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(184..1569)
                     /locus_tag="EYO86_01045"
     CDS             complement(184..1569)
                     /locus_tag="EYO86_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIC41656.1"
                     /translation="MKNKKLNLTEDPVPQLIIKMAVPVSIGFFFHTMFNVVDTYFASQ
                     INSVVVAAITITFPIFFMIIAISSGTRQGATALIANAEGANDKALAKQYLVQTISFSI
                     IAAIFIMIVGLISAPYLFRIMHAEGEYFNFAIQYTNILLYGCLFIILDSTPTAGLNAV
                     GDTKTYARVFIIGFFLNLILDPLFIYGYGPIPSMGIKGIAYATVFAEFAATIYVFYRI
                     KKMTNFFDQVTIKDFLPQIKLQLAIAKQAFPASINMFCVAAGFFIITFFVSFFPTTDT
                     SNVSIAAYGIAIRIEQIILLPAIGLNFACLSLTGQNYGAAKYDRIREGYLVCLKYGLI
                     LLFFGSLLLFFGAGYFMSIFTNDEKVIKIGINYLQIAALFTPIIAVLNISIALMQGVK
                     KPSFTVFISLFKEVIGAVIIIWLICFYFNYQLQGIWFAILLINYLSVIMFLLIVNYKM
                     KSLGLKIFKIK"
     gene            complement(1635..>2204)
                     /locus_tag="EYO86_01050"
     CDS             complement(1635..>2204)
                     /locus_tag="EYO86_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC41657.1"
                     /translation="VIASKCRLNLEHLRRYPHAFSGGQRQRISIARALVSNPQFIVAD
                     ESVAALDVSIQADILNLLKALQKDLNLTYLFISHDLSVVAHTCDEVAVMYLGRIVEIA
                     PTRELFSNPKHPYTKALLSSIPSIDPDKQNKAIELKGEIPSSMNPPSGCVFHTRCQYV
                     NENCKKTVPQLEKLNGSDHQVSCLRINEI"
BASE COUNT          827 a          348 c          334 g          695 t
ORIGIN      
        1 gattactatc catggcaaca aaatttattt ttggatgatc ccttttcggt gaaagaaggc
       61 tgtgtaacaa taacggatat gccaggttgg ggtgtaataa ttaatccaga atggttagaa
      121 aactctgagt atgccgtttc agaaatgcaa tgaataaatt ttttcttttg taaaatacat
      181 atattattta attttaaata tttttaaacc aagagatttc attttataat ttacaattaa
      241 taagaacata attactgaca aataatttat taaaagtatt gcaaaccaaa tgccttgtaa
      301 ttgatagtta aaataaaaac aaattaacca aataataatt actgctccta tcacctcctt
      361 aaataggcta ataaatactg taaagctggg ctttttaact ccttgcatca aggcaataga
      421 gatgtttaaa accgctatga ttggtgtaaa taatgctgct atttgtaaat aattaatacc
      481 aatttttatt actttttcgt cattcgtaaa tatactcatg aaataaccag cgccaaaaaa
      541 aagtaataag ctcccaaaga ataataatat taatccatat tttaaacaca ccaaataccc
      601 ttctcgaatt cggtcatatt tagcggcacc ataattttga cccgttaaag aaaggcaagc
      661 aaaatttagg ccaatagcag gtaataaaat aatttgttct atacgaatag ctattccgta
      721 tgctgcaata gaaacattgc tcgtatctgt ggtagggaaa aaagaaacaa aaaaagtaat
      781 aataaaaaaa ccagcagcca cacaaaacat atttatggat gcaggaaagg cttgtttagc
      841 aatagcaagt tgtaatttta tttgtggcaa aaaatcttta attgtaactt gatcaaaaaa
      901 attagtcatt tttttaatcc tataaaatac ataaatggtt gcagcaaact ctgcaaaaac
      961 agtggcataa gctatacctt taatccccat tgaaggtata ggaccatatc cataaataaa
     1021 taaaggatct aaaattaaat ttaaaaaaaa accaataata aaaacacggg cataagtctt
     1081 agtatctccc acagcattca aacctgctgt cggtgttgaa tccaaaataa taaataaaca
     1141 tccgtacaaa agaatatttg tatattgaat tgcaaaatta aaatactctc cttctgcatg
     1201 cattattcta aataaatacg gtgctgatat caaaccaaca atcatgataa aaatagctgc
     1261 tataatagag aagctaatag tttgaactag gtattgcttt gccaatgctt tatcattagc
     1321 cccttctgca ttcgctatta aggcagtggc gccttgacgt gtgccggatg aaatagcgat
     1381 aatcataaag aaaattggaa atgtaattgt gatagcggca acaaccactg aattaatttg
     1441 tgacgcaaaa tacgtatcaa ccacattaaa cattgtatga aagaaaaatc caatactgac
     1501 aggcactgcc atttttatta taagttgagg gacaggatcc tctgttaaat ttaatttttt
     1561 atttttcata aatttaataa agtcctgata aggataaaaa aacagttaat aataaattat
     1621 ttctcaaaaa aaaattaaat ttcgttaatg cgcaaacatg atacttgatg atcacttcca
     1681 tttaattttt ctaattgagg gacagttttt ttacaatttt catttacata ttgacaacgt
     1741 gtatggaata cacagcctga aggaggattc attgagcttg gaatttcacc ttttaattct
     1801 attgctttgt tttgtttatc agggtcaatg gaaggaatag aggataaaag tgctttcgta
     1861 taggggtgtt taggatttga aaaaagctct cttgtaggag caatctcaac tatacgacca
     1921 agatacataa ccgctacctc atcacatgtg tgggcaacaa cacttaaatc atgactaata
     1981 aataaatacg ttaagtttaa atctttttga agagctttta acaaatttaa aatatctgct
     2041 tggatagaaa catctaaagc tgctacactt tcatctgcca caataaattg aggattacta
     2101 acaagtgctc ttgcaattga gatacgctgt cgttgtcctc ccgaaaaagc atggggatat
     2161 cttcttagat gttctaaatt taatcggcat tttgatgcaa tcac
//
LOCUS       DTVV01000087            1244 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1246711, whole genome shotgun sequence.
ACCESSION   DTVV01000087 DTVV01000000
VERSION     DTVV01000087.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1246711"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="EYO86_01055"
     CDS             <1..687
                     /locus_tag="EYO86_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41658.1"
                     /translation="IIMPRIILSFILILLGLYIGNYIDQNLINQTGQWFWTSLVMLGY
                     IILSVFFVSKYLEKFSGYNRKTSIFSAAPGALGPLLILAEHEKSDLSKVATSHLIRLI
                     IIITLFPFIVDNFSKLSSNSVAEFNFLNQNHFNLLTLFVASIIFIQIFDKFKIPAPLL
                     SGTLVACGILQIADVASYKLPDQSINFCLLILGASVGCRFADKSLSNDTWMSCVNNLL
                     GTSKTHLRYK"
     gene            687..>1244
                     /locus_tag="EYO86_01060"
     CDS             687..>1244
                     /locus_tag="EYO86_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIC41659.1"
                     /translation="MALRILLVDDELNVLKSLKRVLVRSGYDVITASSGFEALEILAA
                     NQVEIIITDFRMPEMNGADLLRQVKINWPDCVNLVLSGYADFKSVVELLNQGLVFRFL
                     EKPWVDSELLENISEAKVKYEQRRAKRVRDQLLMGSMSGLIEIDNNGIINRFNAPAAE
                     IIPDIHLYENKHLAYLDTAQHKAVSQ"
BASE COUNT          398 a          184 c          222 g          440 t
ORIGIN      
        1 attattatgc caagaattat tttaagtttt atcttaatcc ttttgggctt atatatcggt
       61 aactacattg atcaaaatct aattaatcaa acaggacaat ggttttggac ttcacttgtc
      121 atgcttggat acattatttt aagtgtgttt tttgtatcaa aatatctaga aaaattttca
      181 ggatataata gaaaaacttc tattttttca gctgcaccag gagctttggg accattatta
      241 attttagctg agcatgaaaa atcggatctt tcaaaagttg caacttcaca tctaattaga
      301 ctaattataa ttattactct gtttccattt atagtcgaca atttttcaaa gttaagttct
      361 aattctgtag ctgaatttaa ctttttaaat caaaatcatt ttaatttact aactcttttt
      421 gttgcttcta ttattttcat tcaaatattt gataaattta aaatcccagc tcctttatta
      481 tctggaacgt tggttgcatg tggaatattg caaatagctg acgttgcttc ttacaaactt
      541 ccagatcaaa gcatcaactt ttgtttacta attttgggag cttctgttgg atgcagattt
      601 gcagataaat cattaagtaa cgatacatgg atgagctgtg tgaacaattt actaggcaca
      661 agtaaaactc atttgaggta taaatagtgg ctttgcgaat tttattggta gacgatgaac
      721 ttaatgtatt gaaatcttta aagcgagtac ttgttcgttc tggttatgat gtaataacag
      781 catccagtgg ttttgaggca cttgaaatat tagcggctaa tcaagttgag ataattatta
      841 ctgactttag aatgccagaa atgaacggcg ctgatttatt acgccaagtt aaaattaatt
      901 ggcccgattg cgtaaattta gtgctcagcg ggtatgctga ttttaagtct gtggttgagt
      961 tattaaatca gggattggta tttcgttttt tagaaaagcc atgggtcgat agtgagttac
     1021 ttgagaacat tagcgaagca aaagttaaat atgaacagcg ccgagctaaa agagtaagag
     1081 atcagttatt aatgggcagt atgtctggcc taatagagat agacaataac ggaattatta
     1141 atcgttttaa tgcacctgcc gctgaaatca ttcctgatat tcatctatat gaaaacaaac
     1201 atctcgccta tttagataca gctcaacaca aagcggtaag tcag
//
LOCUS       DTVV01000088            2937 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1246930, whole genome shotgun sequence.
ACCESSION   DTVV01000088 DTVV01000000
VERSION     DTVV01000088.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1246930"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="EYO86_01065"
     CDS             complement(<1..1189)
                     /locus_tag="EYO86_01065"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIC41660.1"
                     /translation="MISRYNRPKIEAIWSNENKFRIWTEIECLIAEQLGILGIIPKEA
                     AKDIRKNAKFNVDEINEIEKETHHDVIAYIDNVSNYIGENSKYFHHGVTSSDIVDTSF
                     SIQLKQSSEIIIDQLKELIAELKKKSKVHKNTIMIGRSHGIHAEPITFGLKMASFYFE
                     FKRNLKRLEFALKDISICAISGPVGTFNTIDPSVEQYVAKKLNLRTEDISTQIIPRDR
                     HAHFFSTLGIIASSIERLAIEIRHLQRTEILEVEEFFKEGQKGSSAMPHKRNPILSEN
                     LTGIARYIRSAVIPSMENIALWHERDISHSSVERIFSPDITIATDFALARLTDVIKNL
                     IVYPNNMLKNIHQLGGLHKSQNILLALTQKGLSRQKAYTIVQSSAMKSWNSKEKFEDI
                     IRLN"
     gene            complement(1204..1995)
                     /gene="map"
                     /locus_tag="EYO86_01070"
     CDS             complement(1204..1995)
                     /gene="map"
                     /locus_tag="EYO86_01070"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020021357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIC41661.1"
                     /translation="MQNNIPIHSTADFEGMRKAGSLTSRILDELEQLITPGISTLEIN
                     NFCHKMIIENDAIPAPLNYKGFPKSICTSVNHVVCHGIPSQKKNLQNGDIINIDVTVI
                     LDGWFGDCSRMFVAGKTNKKTYSFLKTTYECLLIGIKNAVPGKSLGDIGYEIQKYAEK
                     NNYSVVRDFCGHGIGKKFHTPPSVLHYGNLGEGPELKPGMFFTIEPMINMGDWKIKIL
                     KDGWTAVTKDKSLSAQFEHTIGITETGNEIFTLSHNNSDFPIKPL"
     gene            2061..2831
                     /locus_tag="EYO86_01075"
     CDS             2061..2831
                     /locus_tag="EYO86_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIC41662.1"
                     /translation="MLFCLKDYMSKLTAGIIVIGDEILSGRTQETNSNFIAKQLVQSG
                     IMLVEIRIVEDNRDTIIQNILHFHKKYTYVFTTGGIGPTHDDITSESVSSAFNEKYCF
                     HPQAFKILEEYYPKGEFNEGRQKMAKMPENAKLILNPLTAAPGFIIKNIYVLPGVPEI
                     MQKMFVNLLQNIEKGSPKKILTINTNLYESIMATGLTAIQNKYLDCSIGSYPYFNYID
                     KTTGVNIVVSSWNLNNLDYIEEEIKKMILLLGGKSSIV"
BASE COUNT          956 a          447 c          459 g         1075 t
ORIGIN      
        1 cattaagacg tattatgtct tcaaattttt ctttagaatt ccatgatttc atagcggatg
       61 actgaactat tgtgtaagct ttttgcctgg ataaaccttt ttgagttagg gctaacagaa
      121 tattctgact tttgtgcaag ccccctaatt gatgaatatt ttttaacata ttattaggat
      181 aaacaattaa attttttatt acatcagtca atcttgcgag cgcgaagtca gtagctatag
      241 tgatatctgg agagaatatt ctttcaacac ttgaatggct tatgtctctt tcatgccata
      301 gtgctatatt ctccatacta ggaatgacag cacttctaat gtatcttgca atgcctgtta
      361 aattttcact taaaataggg ttgcgtttat ggggcattgc agaagatcct ttttgacctt
      421 ctttgaaaaa ctcctctact tccagaattt cagttctttg tagatgtctt atttctatgg
      481 ctaatctttc tatagaggag gcaataattc ctagagttga aaaaaaatga gcatgcctat
      541 ctcgaggaat aatttgcgta gaaatatctt ctgtacgcaa gtttaatttt ttagcaacat
      601 actgttcaac acttggatca attgtattaa aggtgccaac aggtccagat atggcacaaa
      661 tagagatatc ttttaaagca aattctaatc ttttgagatt acgtttaaat tcaaagtaaa
      721 atgaagccat ttttagtccg aaagttattg gttcagcatg aattccatga cttcttccaa
      781 tcattatagt atttttgtga accttagatt tttttttaag ttcagcaatt aactctttta
      841 attgatcaat tattatttcg gaagattgtt tgagttgaat tgaaaaagaa gtatcaacaa
      901 tgtcacttga agtaactccg tgatgaaaat acttagaatt ttcaccaatg tagttactta
      961 cattatcaat gtaagcgatt acatcatggt gagtctcttt ttcaatttca tttatttcat
     1021 caacattaaa ttttgcattt ttacgaatat cttttgctgc ctcttttgga attatgccaa
     1081 ggatacctaa ttgttctgca attaaacatt caatttcagt ccaaattcta aatttatttt
     1141 cattagacca gattgcttca attttggggc gattatatct tgagatcatt atattatagt
     1201 tctttataaa ggttttattg gaaaatcgga attattatga gagagggtga aaatttcgtt
     1261 accagtctct gttatgccaa tagtgtgttc aaattgggca gataaagatt tatcttttgt
     1321 cacagcagtc caaccatctt taagaatttt aattttccaa tcgcccatat taatcattgg
     1381 ttcaatagta aaaaacattc caggtttaag ttcaggccct tcacccaaat ttccataatg
     1441 taacacactt ggaggcgtgt ggaatttttt tccaattcca tgaccacaaa agtctcttac
     1501 aacggaataa ttattctttt cagcatattt ttgaatttca tatccgatat cacctaatga
     1561 cttcccaggg acagcgtttt taattcctat taataaacat tcatatgttg tttttaagaa
     1621 tgagtaagtt tttttgtttg ttttacctgc tacaaacatt ctggaacaat ctccaaacca
     1681 accatctaaa ataacagtca catctatatt aataatatct ccattttgta aatttttttt
     1741 ttgacttgga atgccatgac acactacatg atttacagaa gtgcatattg atttaggaaa
     1801 tcctttgtaa ttaaggggtg cggggatagc atcattttca attatcattt tatggcagaa
     1861 attatttatt tctaaagttg atattccggg agtaattaac tgttctagtt catccaatat
     1921 tctagaagtt aaagatcctg cttttctcat accttcaaaa tcagcagttg aatgaatggg
     1981 aatattattt tgcatctaaa atatttatta tactaattta tagtttgatt aaatatataa
     2041 gaaaaattta ttttatttaa atgctgtttt gtttaaaaga ctatatgagt aaattaactg
     2101 caggcataat tgtaattggg gatgaaatcc tttcaggaag aacacaagaa actaattcta
     2161 attttatagc taaacaatta gttcagtctg gaattatgtt ggttgaaatt aggattgtag
     2221 aggacaatag agatacaata attcaaaata ttcttcattt tcacaaaaag tatacttacg
     2281 tgtttactac aggaggtatt gggcctaccc atgatgatat tacatcggag agtgtatcgt
     2341 ctgcttttaa tgaaaaatat tgttttcacc cacaagcttt taaaatttta gaagagtatt
     2401 atccgaaagg tgaatttaat gaaggaaggc aaaaaatggc aaaaatgcca gaaaatgcaa
     2461 agcttatttt aaacccctta actgctgcac ctggatttat aattaagaat atttatgttc
     2521 tgccaggtgt gccagaaatt atgcaaaaaa tgtttgttaa tcttttacaa aatattgaaa
     2581 agggttctcc taaaaaaatt ttaactatta atacaaattt atatgaaagt attatggcaa
     2641 ctggcttaac cgctattcaa aataaatatt tagattgctc tattggcagt tatccttatt
     2701 ttaattacat tgacaaaact actggtgtca acattgttgt ctcttcatgg aatcttaata
     2761 atttagatta tattgaagag gaaattaaaa aaatgatttt attattgggt ggcaaaagtt
     2821 caatagtctg ataaatgcta aatataggcc tcgtggtgga atggtagaca caaaggactt
     2881 aaaatccttg cccctctagg taaagttcag ctctaagctc cgttttttat acttcgc
//
LOCUS       DTVV01000089            1033 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1247929, whole genome shotgun sequence.
ACCESSION   DTVV01000089 DTVV01000000
VERSION     DTVV01000089.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1247929"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1033)
                     /locus_tag="EYO86_01080"
     CDS             complement(<1..>1033)
                     /locus_tag="EYO86_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIC41663.1"
                     /translation="VNPNLIDDKSNLVYLCAAGVCFMFLVALNSKLRNKNWFKLNNII
                     EPDILNFLDLVCLGTICDVVPVIGLNRAIVKQGLKVFGKRKNIGLKTLYDNSKIKNQP
                     NTYHLGYLIGPKINAGGRVGKSSFGAELLISDNQEKVDYLAKELNNFNEQRKKIEFEL
                     IQKIENETKYSKDPILLIQGKDFHEGIIGIAASRIKDKYKKPCIIISVNGKIGKGSAR
                     SVFGFDIGSIIISAVQNRILIKGGGHKMAGGFSININNIETFKEFLIKKYNNLGIKND
                     NLKNLYLDSLILPTALNVEFYKNIEILSPFGAGNPEPKFIIEDLKLANSNIIANKHIK
                     AIFYSKSNEV"
BASE COUNT          338 a          170 c          116 g          409 t
ORIGIN      
        1 aacttcatta cttttactat aaaatatagc tttgatatgt ttattagcaa taatgtttga
       61 atttgctaat tttaaatctt caattataaa ttttggctca gggttgcctg cgccaaaagg
      121 agataggatt tcaatatttt tgtaaaattc aacgttcaat gcagttggca aaattaaaga
      181 atctaaatac aagtttttta aattatcatt cttaatacct aaattattat attttttaat
      241 taaaaattct ttaaatgttt caatgttgtt aatatttatt gaaaatcctc cagccatctt
      301 atgtcctccc cctttgatta aaattcgatt ttgaacagct gaaataatga tagatccaat
      361 atcaaaacca aaaacagacc ttgccgagcc tttaccaatt tttccgttga ctgaaattat
      421 tatgcaaggc tttttgtatt tatctttaat tcttgatgct gctattccta ttattccttc
      481 atgaaaatct ttgccttgaa tcagtaaaat aggatctttg ctatattttg tttcattttc
      541 aattttttga ataagttcaa attcaatttt tttcctttgt tcattaaagt tatttagctc
      601 tttcgctaaa taatctacct tttcttggtt atcagaaatt aataattctg ctccaaatga
      661 tgattttcca actcttccac ctgcgttgat ttttggacca attaaataac caagatgata
      721 ggtattaggt tggtttttta ttttgctatt atcatataaa gtttttaaac caatattttt
      781 tcgtttccca aaaactttta acccttgttt aactatagct ctatttaacc ctataacagg
      841 aacaacgtca caaattgttc ccaaacaaac taaatctaaa aaatttaaaa tatcaggttc
      901 aataatatta tttagtttga accaattctt gtttctaagt ttgctattca gcgctaccaa
      961 aaacataaaa caaactcctg ccgcacagag atataccaag ttagatttat catcaatcag
     1021 gtttggattt aca
//
LOCUS       DTVV01000090            1256 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1248049, whole genome shotgun sequence.
ACCESSION   DTVV01000090 DTVV01000000
VERSION     DTVV01000090.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1248049"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            46..>1256
                     /locus_tag="EYO86_01085"
     CDS             46..>1256
                     /locus_tag="EYO86_01085"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIC41664.1"
                     /translation="MPKINVKNPVVELDGDEMAAIIWDFIKKKLILPYLDINIKYYDL
                     GIKNRDITSDRITVDCAKAIKKIGVGIKCATITPDESRVKEFKLKKMWKSPNGTIRNI
                     LGGTVFREPIICKNIPKLVPGWTNPIVIGRHAFGDQYRATDFKVPGKGKLEIKWTSED
                     GKEKKEFEVFNFDGPGVALAMYNLDQSIKDFARACMNYGLNRKWPVYLSTKNTILKKY
                     DGRFKDLFQEVYEKEFKEKFKERKITYEHRLIDDMVACAMKWNGNYIWACKNYDGDVQ
                     SDTIAQGFGSLGLMSSVLMLPDGKTIESEAAHGTVTRHYRLHQQGKETSTNPIASIFA
                     WARGLYHRGKLDGNEDLKKFVKTLEKVCIQTVESGSMTKDLAMLIGKNQKYLTTNQFL
                     EVINSNLKRSL"
BASE COUNT          497 a          166 c          233 g          360 t
ORIGIN      
        1 atatgtatat ttaataattt tattcaattg ataaaataaa tattaatgcc caaaattaat
       61 gtaaaaaacc cagttgtaga gctagatggc gatgaaatgg ccgcgataat atgggatttt
      121 attaaaaaaa agctaatcct tccctacctc gacatcaata ttaaatacta tgatcttggt
      181 ataaaaaaca gagatataac atctgatcga ataacagttg attgtgctaa agctataaaa
      241 aaaattggcg taggtattaa atgcgcaaca attactcccg atgaaagccg tgtaaaggaa
      301 tttaaattaa aaaaaatgtg gaaatctccc aatggaacaa ttagaaatat cttaggaggg
      361 acagtgtttc gtgaaccaat aatttgcaaa aatattccaa aattggttcc tggctggaca
      421 aatccaatcg taattggaag gcacgctttt ggtgatcagt acagagcaac agattttaaa
      481 gtacctggta aaggtaagct agaaattaaa tggacttcag aagacggaaa agaaaaaaaa
      541 gaatttgaag tttttaactt tgatggaccc ggtgttgcgt tagcaatgta taatttagat
      601 caatctatta aagattttgc aagagcttgt atgaattatg gacttaacag aaaatggcca
      661 gtttatcttt ctactaaaaa tacaatttta aaaaaatacg atggtaggtt taaagattta
      721 tttcaagaag tttatgaaaa agaatttaaa gaaaaattta aagaaagaaa aataacttat
      781 gagcacagac ttatagacga tatggttgcg tgtgcaatga aatggaatgg aaattacatt
      841 tgggcatgta aaaactatga tggggatgtg caatctgata cgattgctca gggatttggc
      901 tcattaggtc ttatgtcaag tgttttgatg ttaccagatg gtaaaactat agaatctgaa
      961 gctgcacatg gtactgtaac aagacactac agattacatc aacaaggtaa ggaaacttca
     1021 acaaatccca tagcttctat ctttgcatgg gcaagaggtc tatatcatag aggaaagcta
     1081 gatggtaacg aagatttaaa aaaatttgta aagactttag aaaaagtttg catacaaacg
     1141 gttgaaagtg gttctatgac caaagatcta gctatgctaa tcggtaaaaa ccaaaaatat
     1201 ctaaccacaa atcagtttct agaagtaata aattctaatt taaaaagaag ccttaa
//
LOCUS       DTVV01000091            3212 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1251559, whole genome shotgun sequence.
ACCESSION   DTVV01000091 DTVV01000000
VERSION     DTVV01000091.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1251559"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="EYO86_01090"
     CDS             complement(<1..359)
                     /locus_tag="EYO86_01090"
                     /inference="COORDINATES: protein motif:HMM:PF00106.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC41665.1"
                     /translation="MKKILVTGGSGKAGKATIKKLLEKNYKVFNVDIEPTSEFDVPFT
                     RVDLESFGETMEAVTKIDDRNKGIDAIIHQAAIPADGLYPNHKTFRINTLSTYNVFQC
                     AEIMKINNIVWASSETV"
     gene            complement(419..2587)
                     /locus_tag="EYO86_01095"
     CDS             complement(419..2587)
                     /locus_tag="EYO86_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIC41666.1"
                     /translation="MKLDNVYEEKVYAGVLGKIIGVYLGRPFEGWTYDRIMSELGPIN
                     YYVHDKLKVPLIVTDDDITGTFTFIRALNDYNYNYNVTSRQIGQTWLNNIIEKETILW
                     WGGVGNSTEHTAYQNLINGIPAPESGSIKKNGKVIAEQIGAQIFIDGWAMVCPGDPEK
                     AIDLAGRAAKVSHDGEAVFGAQIIAALESLAFIENDINKLLNVSKKLIPNTSTIYKLI
                     NDIQDWHAGNSDWKRAREKIQDKYGYDKFLGNCHMVPNHALIIMSLLYGEDNFQKSLM
                     IVNTAGWDTDCNSGNVGCILGIKNGLQGIDSGPDWRSPVADRLYLPTANGGQSTTDAV
                     TETYKLIKIAKLMNHQKFEAPKTGYRYHFEMPGSLQGFKVDLTSKNNQLTYIKNVEGH
                     SKSGKRSLQITYDHLSNGVDSKVYVDTFIPKEIHELEGHARNVFFHYNLYTCSNIYSG
                     QIIKAKIIADKNNKQNIFCNLYIRCYSKADKLISIESEKKLIKVNSINDFSWKLPGTN
                     GDIISQIGLRISSSENSQGKIYLDYLGWEDEPKVSFFKPNHVVPFQRGQPSNKIKNSE
                     MWRNNWVKATDHWDIHWKEAFHISNNNGRGMIITGTNDWKNYTVSSKITFHLMTSGGI
                     AARVQGLRRYYSLEASKQNKIRLVKMFEEIDILEEKDFAIQLHKEYLFSLSVKDNFIR
                     GLVDGILILEYNDDSNMFQTGGIGLVIENGTLSTNEILVN"
     gene            complement(2590..>3212)
                     /locus_tag="EYO86_01100"
     CDS             complement(2590..>3212)
                     /locus_tag="EYO86_01100"
                     /inference="COORDINATES: protein motif:HMM:PF00528.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC41667.1"
                     /translation="LNSFQLVIGTLIICLLFSGLAAFSLARLKPKGAMIFAIYLLVGI
                     SIPAQMFILPLFLLWKNLNLIDTHIGLIIIYSGLNAPFATFLIRSYMIQLPEELFDAA
                     RIDGANPFQLFAKIAVPLSWPVFLTAGLIVGLAVWNEFLFALTFMQDENSKPISTILF
                     AFSTRFENNWGLVSASAVMMVAPVAILFMIFQRRFIAGLTSGGLKG"
BASE COUNT         1048 a          590 c          434 g         1140 t
ORIGIN      
        1 aaaactgttt cacttgaagc ccatactata ttatttattt tcattatttc agcgcattga
       61 aaaacattgt aggtgctgag cgtattaata cgaaatgttt tatgattagg gtagagccca
      121 tctgctggga tggctgcttg atgtataata gcatctattc ctttattcct atcatctatt
      181 ttagtgacgg cttccatggt ttcaccaaaa ctttctaaat ctactctagt aaaaggaaca
      241 tcaaattcac tggtcggctc aatatcaaca ttaaatactt tataattttt ttctaaaagt
      301 ttttttattg tagctttacc tgctttacca gagccacccg taacaagaat ttttttcatt
      361 atattatcct accaccaacc acgaggaatg ttgagaataa agatattgat taaaatattt
      421 aatttactaa tatttcattt gttgaaagtg ttccattttc aatgactaat cctatacctc
      481 cagtttggaa catattagaa tcatcattat actccagtat taatatccca tcaacaagcc
      541 cccggatgaa attatctttt actgataaag aaaaaagata ttccttgtgc aattggattg
      601 cgaaatcttt ttcttcaaga atatcgattt cttcgaacat tttcaccaat cgaattttat
      661 tttgcttaga tgcttctaat gaataataac gtcttaaccc ttgaactctt gcagctattc
      721 ctccagatgt cataagatga aatgtaattt tagatgatac cgtataattt ttccaatcat
      781 tagtgccggt aataatcatc cctctcccgt tattattaga aatatgaaaa gcttccttcc
      841 aatgaatgtc ccagtgatct gttgctttaa cccagttatt tctccacatc tctgagtttt
      901 ttattttatt acttggttgg cctctttgaa atgggacaac atgattaggt ttaaaaaaag
      961 atactttggg ttcgtcttcc caccctaaat aatcaagata tatttttcct tgagaatttt
     1021 cggatgaact aattcttaat cctatctgag atatgatatc accatttgtg cccggtaatt
     1081 tccaagaaaa atcgttaatt gaattaactt taattagttt tttttcagat tcaatggata
     1141 tcagtttatc agcttttgaa taacacctaa tatataaatt gcaaaaaata ttttgtttat
     1201 tgtttttgtc tgcaattatt tttgctttta tgatttgtcc tgaataaata ttggagcatg
     1261 tatataaatt gtaatgaaaa aaaacatttc ttgcatgccc ttctaattca tgaatttctt
     1321 ttggtataaa agtatctaca tatactttgc tatcaactcc attacttagg tgatcataag
     1381 ttatttgaag cgatcttttt cctgatttac tatgaccttc aacatttttt atataagtaa
     1441 gttgattatt ttttgaagtt aaatcaacct taaatccttg aagactccct ggcatttcaa
     1501 aatgatatct atatccagtt tttggtgctt caaatttttg atgattcatt aattttgcaa
     1561 tttttattaa tttgtatgtt tctgtgacag catcagtggt actctgacca ccattagctg
     1621 ttggtaaata taatctatca gcaacaggag aacgccaatc tggacctgaa tcaatacctt
     1681 gaagtccatt ttttatacct aagatacaac ctacattacc tgaattacaa tctgtatccc
     1741 aacctgctgt attgactatc attagtgatt tttgaaagtt atcttccccg tataaaagag
     1801 acataataat taaagcatga tttgggacca tatgacaatt gcccaaaaat ttatcatatc
     1861 catacttatc ttgaattttt tctcttgccc ttttccaatc actattgcct gcatgccaat
     1921 cttgaatatc attgataagt ttgtaaattg ttgatgtatt tggaattagt ttttttgata
     1981 catttaaaag tttattaata tcattttcta taaaagctaa tgattctaat gcagcaatta
     2041 tttgtgcacc gaagacagct tctccatcat gacttacttt tgctgctctt ccagctaaat
     2101 caatagcttt ttccggatca ccggggcaca ccatggccca accatcaata aatatttgtg
     2161 ctccaatttg ttctgcaata actttgccgt tttttttaat tgatccactt tcgggtgccg
     2221 gaataccatt aattaaattt tgataagccg tatgctctgt tgaattacca actcctcccc
     2281 accaaaggat agtttctttc tcaataatat tattcagcca agtctgacct atctgtcttg
     2341 aggttacatt ataattatag ttatagtcat tcaaagctct aataaatgta aatgtgccag
     2401 taatatcatc atcggttact attaatggaa ctttaagttt atcatgaacg taataattaa
     2461 taggacccaa ctcactcata atacgatcat aggtccaacc ttcaaatgga cgccctaggt
     2521 atacaccaat aatttttcct aaaactccag cataaacctt ttcttcataa acattatcta
     2581 gtttcatatt tatcctttaa gcccaccact tgttaaccca gcaataaaac gacgctggaa
     2641 tatcataaat aaaattgcta ctggcgctac catcatgact gcagaagcac taactaaacc
     2701 ccaattgttt tcaaaacgtg ttgaaaaagc aaagagaata gttgatattg gttttgaatt
     2761 ttcatcttgc ataaaagtta gggcaaataa aaattcattc caaactgcta atccaactat
     2821 caatcctgct gttaaaaaaa caggccaaga tagaggtact gcaatttttg caaataactg
     2881 aaaaggattg gctccatcaa ttcgagctgc atcaaacaac tcttcaggga gttgtatcat
     2941 ataagatcta atcaaaaaag ttgcaaaagg agcatttaac ccactataaa taatgattaa
     3001 accaatatga gtatcaatta gatttaaatt tttccacaat aaaaacaaag gtaatataaa
     3061 catttgcgca ggaatactaa tgcctaccag taaatatatt gcgaaaatca ttgcaccttt
     3121 aggcttaagt ctagccaaac taaacgcagc taaacctgaa aacaaaagac agataatcaa
     3181 agtgccaatt actaattgaa atgaatttaa aa
//
LOCUS       DTVV01000092            1920 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1254239, whole genome shotgun sequence.
ACCESSION   DTVV01000092 DTVV01000000
VERSION     DTVV01000092.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1254239"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            94..651
                     /locus_tag="EYO86_01105"
     CDS             94..651
                     /locus_tag="EYO86_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_437643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIC41668.1"
                     /translation="MDSKHKPTVGIETTNPKSMPKEHSEIPVWNSENWFYEDVIIGHK
                     IRSIRRTISEGESMQFNCMVLDMHPYVGDEIFAKEEGMFNNRLVAGAMVFSYGLGLVA
                     TNCVNSFSYGYDRLRFIKPVFIGETIYTIRANMEKTPKYEKMGLIRTSYEVFKGEGEI
                     VLYCEHLHSVVYKEPEKFKDQIIKK"
     gene            652..1785
                     /locus_tag="EYO86_01110"
     CDS             652..1785
                     /locus_tag="EYO86_01110"
                     /inference="COORDINATES: protein motif:HMM:PF01547.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC41669.1"
                     /translation="MIALKGMTWDHPRGFDPMVATSESFKKKHNNFISIAWDKRPLQA
                     FADRPIEQMTEDYDLIVIDYPHLGEVAANGLLDYFDKPQYLDQLSKLKKESVGNSHQS
                     YFVDDHQWALAIDAATQVSVFRKDLINFLPSSWNELVELAKNNKILWPLKPIHAISSF
                     YSICNNIDKPFNPDLKEFINKKTTIRTLMMMKEIVKYLPNECLQMDSIQACEYMAENN
                     DIFYCPYIYGFSNYSRYGFKKNVLLFSNVIDLEGNGPVGTQLGGTGIAVSKASSFKEE
                     AFEYALWVASSDCQKNIYYKNGGQPGNAVAWENEIINKETNNFFSSTRDTLEGAWVRP
                     RHNGYMKFQDLGGDIINDFLKNNLNEEDIYDKLNYEFKKSFVQ"
     gene            1782..>1920
                     /locus_tag="EYO86_01115"
     CDS             1782..>1920
                     /locus_tag="EYO86_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIC41670.1"
                     /translation="MSNEIKRPLDGVIVLEFSQFLAGPVAGLRLSDLGARVIKIEKPE
                     VG"
BASE COUNT          694 a          240 c          353 g          633 t
ORIGIN      
        1 caagtttgta atcctcaaaa taaagttcta tttcagccat attgatatat tctaattcta
       61 attatactca ttatcattta atattatttt gtaatggata gcaaacacaa acctacggtc
      121 gggattgaaa ctaccaatcc taaaagtatg ccaaaagaac atagtgaaat tccagtatgg
      181 aattctgaaa attggtttta tgaagatgtt atcattggtc ataaaattcg ctcgattaga
      241 agaacaattt ctgaaggtga gtctatgcaa tttaattgta tggttttaga tatgcatcct
      301 tatgttggtg acgaaatttt tgccaaagaa gaaggaatgt ttaataatag attagtagca
      361 ggagcaatgg tattttcata cggtcttggt ttagttgcaa ctaattgtgt gaattctttt
      421 agctacggtt atgatcgttt aagatttatt aaaccagttt ttatcggaga gactatttat
      481 acgataagag ctaatatgga aaaaactcca aaatatgaaa aaatgggact tatccgaact
      541 agctatgaag tatttaaagg tgagggtgaa atagttttat actgtgagca tttacattcc
      601 gttgtttata aggaacctga aaaatttaaa gatcaaatta taaaaaaata aatgattgct
      661 ttaaaaggaa tgacttggga tcatcctaga ggttttgatc ccatggttgc tactagtgaa
      721 tcttttaaaa aaaaacataa taattttatt agtatagctt gggacaaaag accgctccaa
      781 gcttttgctg acagaccaat tgaacaaatg actgaagatt atgatttaat agtaattgat
      841 taccctcatt taggggaagt agcagctaat ggtttattag attattttga taaacctcaa
      901 tatcttgatc aactttcaaa acttaagaaa gaaagcgttg gcaattctca tcaaagctat
      961 tttgttgatg atcatcagtg ggctttagca attgatgcag caacacaagt ttctgtattt
     1021 agaaaagatt taattaattt tttaccttca tcttggaatg agttagttga attagcaaaa
     1081 aataataaaa tactttggcc gttaaaaccc atacatgcaa tcagcagctt ttatagtatt
     1141 tgtaacaata tcgacaaacc ttttaatcct gacttaaagg aatttataaa taaaaaaaca
     1201 acaattagaa cgttaatgat gatgaaagaa attgttaaat atttacccaa tgaatgttta
     1261 caaatggatt caattcaggc gtgtgagtat atggcagaaa ataatgatat attctattgt
     1321 ccttatattt atggtttttc taattattca aggtatggtt ttaaaaaaaa tgttttattg
     1381 ttctccaatg taatagattt agaaggtaat ggacctgttg gaactcaatt aggtggaacg
     1441 ggcatcgcag tttcaaaagc aagttctttt aaagaggaag cttttgaata tgcgctttgg
     1501 gtggcaagtt ctgattgcca aaaaaatatt tattacaaga atggaggaca acctggtaat
     1561 gctgtagctt gggaaaatga gataataaac aaagaaacta ataatttttt ctctagtacg
     1621 agagatactt tagaaggagc ttgggtacga ccaaggcata atggatatat gaaattccaa
     1681 gatcttggtg gagatattat taacgatttt ctaaaaaata atttaaatga agaagatata
     1741 tacgataagc ttaattatga atttaaaaaa agttttgtcc aatgagcaat gaaatcaaaa
     1801 gaccgttgga cggagttatt gtactagaat tcagtcaatt tttagcagga ccggtagctg
     1861 gtttacgttt gtctgattta ggagcaaggg taataaaaat tgaaaaaccc gaagttggcg
//
LOCUS       DTVV01000093            1412 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1256964, whole genome shotgun sequence.
ACCESSION   DTVV01000093 DTVV01000000
VERSION     DTVV01000093.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1256964"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="EYO86_01120"
     CDS             <1..820
                     /locus_tag="EYO86_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HIC41671.1"
                     /translation="YSISDSDLLLWAMGVAGKDQYDINLVEPDVSPLQVQGPKSKNVM
                     VDLFGSWINDLNYYWFKQTELEDIPVVVSRTGWSGEKGYEIYLRDGSRGSDLYEKIME
                     AGEPYKIAPTGPSQIRRVEAGIFSYFQDMRVTDNPYEIGMDRLVDLEMEADFVGKEAL
                     KIIKQEGIKRKLVGIEILGDPITMIVPPEYTPSYFVPDHWPVNDAQDEEIGHVTSKCF
                     SPRLKKNIGFAFVPIEHANQGNKFFINSPYGKLESVVVELPFWDPKKKIPIGKN"
     gene            864..1136
                     /locus_tag="EYO86_01125"
                     /pseudo
     CDS             864..1136
                     /locus_tag="EYO86_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037639.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
     gene            complement(1242..>1412)
                     /locus_tag="EYO86_01130"
     CDS             complement(1242..>1412)
                     /locus_tag="EYO86_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC41672.1"
                     /translation="FINTYRLISPIAPFGGFKNSGFGRESGMEVIKDYSNVKTTWINT
                     SNEPIGDPFVIR"
BASE COUNT          474 a          222 c          285 g          431 t
ORIGIN      
        1 gtattctatt tctgatagtg acttattact ttgggcaatg ggagttgcgg gcaaggatca
       61 atatgatatt aatttggtag agccggatgt ctcaccctta caagttcaag gccctaaatc
      121 aaaaaatgtt atggtggatt tatttggctc ttggattaat gaccttaatt attactggtt
      181 taaacaaaca gaattggaag acattcctgt tgtagtttct cgcacaggat ggagtgggga
      241 aaaaggttac gaaatttatt tacgtgatgg tagccgcgga agtgatttgt atgaaaagat
      301 tatggaagca ggggaacctt acaaaatagc acccacaggg ccttcccaga ttcgtagggt
      361 tgaggcagga attttttcgt actttcaaga catgcgggta actgataatc cttatgaaat
      421 aggcatggat agacttgttg atttggagat ggaagctgat tttgtaggca aggaggcact
      481 aaaaattatc aagcaggaag gtataaaacg aaaattagtt ggcatcgaaa tattgggtga
      541 tcctatcacg atgattgttc ccccagagta cacccctagt tattttgttc cagatcattg
      601 gccagttaat gacgcacaag atgaggaaat tggccatgtg acttcaaaat gcttttctcc
      661 aagattaaaa aaaaatattg gttttgcctt tgttcctatc gagcatgcaa atcaaggaaa
      721 taaatttttt attaactccc cttatggtaa actcgaatct gtagtagtgg agttaccgtt
      781 ctgggatcca aaaaagaaaa ttccaatagg aaaaaattga aatattgatt aattcaaaat
      841 attattagta gtgaaaggtt tttatgaaat atgttttact cggatcaatt tctccttcct
      901 ggcttggcaa acaggcctaa cggttaaaaa aatcaaatga aaaattaaaa caacttttga
      961 ttaagcaaga tgctgttctt tatacgcagg ggcagtacga ctttgttgaa acgattgatg
     1021 ctccaggtcc agaatcagta ctgggtttta ctatatggta ttccaaaaaa ggatttggca
     1081 acattcagac actgccggct tttggagatc aggaaatacg gaagataata aaaaaaatga
     1141 taaaaatcct caaatatcta aatagggtgt taaggaatat aagtgacatc acttatgcag
     1201 atataataac gccttatata tagtattaat aaaaattcct tctatctgat tacaaaagga
     1261 tcaccgatag gttcgtttga tgtattgatc caggtagttt tgacattaga gtaatctttt
     1321 attacttcca taccagactc tctcccaaaa ccactatttt taaatcctcc aaaaggagct
     1381 atcggagaaa tcaaacgata cgtatttata aa
//
LOCUS       DTVV01000094            2497 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1260769, whole genome shotgun sequence.
ACCESSION   DTVV01000094 DTVV01000000
VERSION     DTVV01000094.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1260769"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="EYO86_01135"
     CDS             <1..324
                     /locus_tag="EYO86_01135"
                     /inference="COORDINATES: protein motif:HMM:PF00834.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIC41673.1"
                     /translation="KAGIAIHPNVEIEAIQEFLNDIDLVVVMTVVPGFGGQKFLDTQL
                     SKINKLYYIRESAKAKFEIEIDGGINEQTSKQCIANGADVLVAGSYIYEGDESEYENL
                     INSIR"
     gene            324..1211
                     /locus_tag="EYO86_01140"
     CDS             324..1211
                     /locus_tag="EYO86_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIC41674.1"
                     /translation="MDWNKLKAFYEVAINKSISKACSKLNISQSAISRQIQDLELDLK
                     TALFTRHQKGVRLTEQGENLFEAVNKINFSISDFEQKLLDKKTKPVGKLTVSTTIGFG
                     STWLTPRINKFTNQYPDMEISLLMSDDEIDLANRSADVAVRVKRPTQANLIFKKFVNF
                     HNHIYASSDYLQSNGIPRNTNDLDKHNLICFGSGLPSPISNIDWILKVGRESGKRRPK
                     FRVNSIYGISLAIEKGAGLASLPDYMVADKPNLVRVLPNLEGPSYQTYFVYTEAFKND
                     RRLEVFRDFLYSEAQDWHY"
     gene            1429..1821
                     /locus_tag="EYO86_01145"
     CDS             1429..1821
                     /locus_tag="EYO86_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004388864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIC41675.1"
                     /translation="MPILKTVSEKKAKGKVKKIFDTIKKTRKINTVPNFWKTIAADPD
                     TLERTWNSLQQVMKKGSLDPLTKELIYVAVSMTNSCEYCIRSHSLAAKKKGMTDKMLK
                     ELIAIVGMANETNRLVESYQVEVDKLLK"
     gene            1896..>2497
                     /locus_tag="EYO86_01150"
     CDS             1896..>2497
                     /locus_tag="EYO86_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIC41676.1"
                     /translation="MYKFFTNKKWFLWAYLGSFVILTSLWVSVQIDVKINEWFGEFYD
                     MIQKALGTPNAITMDEYMGGLISFAKLATMWIVLGLATSFLTAHFLFRWRTSMVEWYH
                     SVFDKARTIEGASQRVQEDTIKFSRILESLGTSFIESIMVLIEFFPLLMGLSIGIPIL
                     WFGDWEYSLVAGAFLWAVGGTILMVILAYLLRLVGIEYDL"
BASE COUNT          872 a          362 c          472 g          791 t
ORIGIN      
        1 aaggctggaa ttgcaattca tccaaatgta gaaattgagg caatacaaga atttttaaat
       61 gatattgatc tcgtggttgt aatgacggtt gtaccaggtt ttggtggaca aaaattttta
      121 gacacacagc tgtccaaaat taacaaatta tattatattc gcgagtcagc caaagcaaag
      181 tttgaaatag aaattgatgg cggtattaat gagcaaacaa gcaagcaatg tattgccaat
      241 ggagctgatg tattagttgc aggttcatat atttatgagg gtgatgaatc agaatatgaa
      301 aacctaatta attctatacg ataatggatt ggaataaact taaagcattt tacgaagtag
      361 caataaataa aagtatatca aaagcttgta gcaaacttaa tattagccaa tctgcaatta
      421 gtcgacagat acaagatcta gaacttgatc tcaaaacagc tttattcacg agacatcaga
      481 aaggggtacg tctaactgaa caaggagaaa atttatttga agcagtgaac aaaatcaatt
      541 tttccatttc tgattttgaa caaaaattat tggataaaaa aactaagcct gttggaaaac
      601 taactgtaag cacaaccata ggatttggct ctacctggtt aacacctcgc attaacaagt
      661 ttactaatca gtatcctgat atggaaatca gtttgttaat gagcgatgat gaaattgatt
      721 tagcaaatcg atcagctgat gttgcggtaa gagtaaagag accaacacag gctaatttaa
      781 tttttaaaaa atttgtaaat tttcataatc atatttatgc ctcttctgac tatttacaat
      841 caaatggcat acctcgaaac actaacgatc tggataaaca taatttaatt tgttttggat
      901 ccggcctccc ctctcctatt tccaatattg attggatttt aaaagtaggt agggaaagcg
      961 gcaaacgtcg gcctaagttt cgtgtaaata gtatttatgg catatcctta gcaatagaaa
     1021 aaggagccgg gctggcctcc cttcctgact acatggtagc agataagcct aacttagttc
     1081 gggtacttcc aaatcttgag ggaccgtcct accaaactta ttttgtgtat actgaagcgt
     1141 ttaaaaatga tcgtcgtttg gaagttttta gggattttct ctatagtgag gctcaagatt
     1201 ggcactatta atgttttgac ttaatgttgt tattttgtat atgtacccaa cacaattatg
     1261 ggatatccga agaaacatag aggccgcaga aaaagaggtt ctaaatacag aaaaaataaa
     1321 cgacgtcaga agaaaaaata actaaatctt ttaattaaat ttctaaattc ttattttatc
     1381 attattcata ttaatctatc atccatgaat taatcatgga ggatgaatat gcccatttta
     1441 aagactgtct ctgaaaaaaa agcaaaaggt aaggtaaaaa aaatatttga tactatcaaa
     1501 aaaacaagga agattaatac ggtacctaat ttttggaaaa ccattgctgc agatcctgat
     1561 actttagaac gaacatggaa ttccctgcaa caggtaatga aaaagggttc tcttgaccct
     1621 ttaacaaaag aacttatata cgtagctgtt tcaatgacaa atagctgtga atattgtatt
     1681 cgctcccata gcttagctgc caagaaaaag ggaatgacgg acaaaatgtt aaaggaatta
     1741 attgctattg tgggtatggc aaatgaaaca aacagattgg tcgaatctta tcaggtcgaa
     1801 gttgataaat tgttaaaata atttttttat ctttatgagt aagtaatatc ataatatgct
     1861 actaaaaatt ttacaaaacg ttatagattt ttttaatgta taaatttttt actaataaaa
     1921 aatggtttct ctgggcgtat ttaggatcat ttgtaatttt aacatcgtta tgggtttcag
     1981 tacaaattga tgtaaagata aatgaatggt ttggtgaatt ctacgatatg attcaaaaag
     2041 cattgggaac accgaatgct attacaatgg atgaatatat gggaggccta ataagtttcg
     2101 ccaaacttgc caccatgtgg attgtgttag gacttgcaac atcattttta acagcgcact
     2161 ttttgtttag atggcgtact tcaatggtag agtggtatca tagtgttttt gacaaagcac
     2221 gtacaattga aggagccagt caaagagtgc aagaagatac aattaagttt agtcgcattc
     2281 ttgaaagtct tggaactagt tttatagaat ccataatggt attaattgaa ttctttccat
     2341 tgttaatggg attatcaata ggcattccaa tattatggtt tggcgattgg gaatattctt
     2401 tagtagcagg tgcttttctt tgggcagtag ggggcactat attaatggtg atattggctt
     2461 acttgttaag gctcgtaggt attgaatacg acttgca
//
LOCUS       DTVV01000095            1153 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1262973, whole genome shotgun sequence.
ACCESSION   DTVV01000095 DTVV01000000
VERSION     DTVV01000095.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1262973"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="EYO86_01155"
     CDS             <1..427
                     /locus_tag="EYO86_01155"
                     /inference="COORDINATES: protein motif:HMM:PF14561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC41677.1"
                     /translation="TENPEEFQSMGLYLDCLSHLQKYNEADDFCRSLSKDALSNNYIK
                     SSLQKLEIRKKNIDAPSLENLIKEFKNDPKNLEALCNLADKYFAENMLDEAFELLLNN
                     YRNNKEEIKKKILEFFEALGNDNVKTQEYRKKFSSIMFS"
     gene            435..1049
                     /locus_tag="EYO86_01160"
     CDS             435..1049
                     /locus_tag="EYO86_01160"
                     /inference="COORDINATES: protein motif:HMM:PF02190.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41678.1"
                     /translation="MAKKMLIPIFPLNGAILFPETNLPLNIFEERYIEMIDFALGKNK
                     LLGMIQTKDNGDLYRVGCIGRINSFNETKDGRYIINLRGKNHFSILKEVPLNHKFRLV
                     ESVFENINIIQDKTFELKKFNKELFITKYRPYINRINPGIDFNLINKIEPATLIKFIA
                     MSCPFSSADKQMLLETFNLNDLAHKLITLFEYYSSTSKNERSVN"
BASE COUNT          468 a          167 c          165 g          353 t
ORIGIN      
        1 aactgaaaat ccagaagagt ttcaaagcat gggtctatat cttgattgcc taagtcatct
       61 tcaaaaatat aatgaagccg atgatttttg tcggtcactt agcaaagatg ctctaagcaa
      121 taactatata aagtcatctc tccagaaatt ggagataaga aaaaaaaaca ttgatgctcc
      181 ttcattggag aatttaataa aagagtttaa aaacgatcct aaaaatctag aggcattatg
      241 caatttagcc gataaatatt ttgctgaaaa tatgctggac gaagcattcg agctacttct
      301 taataattat agaaataaca aagaagaaat taaaaagaaa atattggaat tttttgaagc
      361 tctaggaaat gataatgtaa aaactcaaga atatagaaaa aaattttcca gtattatgtt
      421 ttcataattt ttccatggcc aaaaaaatgc ttatacctat ctttccgctc aacggcgcaa
      481 ttctttttcc agaaacaaat ttgcccctaa atatatttga agaacggtac attgaaatga
      541 tagattttgc gctaggtaaa aacaaactat taggaatgat acagacaaaa gataatgggg
      601 atctttatag agttggatgt attgggagaa ttaactcctt taatgaaacg aaagacggaa
      661 gatacattat caaccttaga ggaaaaaatc atttttcaat tttaaaggaa gtgcctttaa
      721 atcataagtt tcgattagta gaatcagttt tcgaaaatat taatataatc caagacaaaa
      781 catttgaatt aaaaaaattt aataaagaat tgttcataac gaaataccgc ccttatatta
      841 atcgcataaa tccaggcata gattttaatt tgataaacaa gattgaacca gctactctaa
      901 ttaaatttat tgctatgtca tgcccatttt cttctgcaga caaacaaatg ttattggaga
      961 catttaactt aaatgacctc gctcataaac taataacatt atttgaatat tattcatcaa
     1021 caagtaaaaa tgaaagatca gttaattaac aaagaatttt atatacgcat actagaaata
     1081 ttagtgtgcc cactctcaaa agataaattt ttttataaca aaaaaacaaa ctctttagat
     1141 tccaaaaagt cgg
//
LOCUS       DTVV01000096            3009 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_126347, whole genome shotgun sequence.
ACCESSION   DTVV01000096 DTVV01000000
VERSION     DTVV01000096.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_126347"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="EYO86_01165"
     CDS             <1..291
                     /locus_tag="EYO86_01165"
                     /inference="COORDINATES: protein motif:HMM:PF10458.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIC41679.1"
                     /translation="SFNAIFSKSDSSAYIVISNSTLIVPLEGVIDTNEEIGKLNLKKK
                     NETSELINLQNKLNNPQFIAKAPKNIIEQFKMQSKEIKSSIEKIDQIINTIK"
     gene            294..2021
                     /gene="ilvD"
                     /locus_tag="EYO86_01170"
     CDS             294..2021
                     /gene="ilvD"
                     /locus_tag="EYO86_01170"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIC41680.1"
                     /translation="MGDKGSNIKSKLPSRHVSVGPKSAPHRSMYYAMGLTEEEIYQPF
                     IGVATTWNESAPCNITLGRQAQAVKIGVKEKLGTPREFTTITVTDGIAMGHEAMKASL
                     VSREVIADSVELSVRGHCYDGIVGLAGCDKSLPGLMMAMVRLNIPSVFIYGGSILPGK
                     YKGKDLTIIDVFEAVGKHALGTIDDNELKAIEQVACPSAGSCGGQFTANTMACISEAL
                     GLALTNSTMTPATYEERDNYGIESGRAVMNLIEKNIRPRDIVTIESLKNAAVVVAATG
                     GSTNAALHLPAIANEAGIKFTLRDVVEIYQKTPYIGDMSPGGKYVAKDLYDVGGVPVV
                     IKSLLDGGYINGDCLTVTGQTIAENHKDTIFPINQDVVYPCNKPISNHSSVVGLWGNL
                     AEDGSISKIAGLSNLHFKGKAKCFDCEEDALSAVLKNEINAGDAVIIRYEGPKGGPGM
                     REMLSTTAAIYGQGLGESVALITDGRFSGGTRGFCIGHVGPEAAEGGMIGLLKDGDEI
                     IIDAIKGTINATLSEEEIEKRKKEWKPRQTNHNSGSIWKYAQTVGPAYKGAVTQPGAK
                     NETHTYADI"
     gene            complement(2025..2813)
                     /gene="xth"
                     /locus_tag="EYO86_01175"
     CDS             complement(2025..2813)
                     /gene="xth"
                     /locus_tag="EYO86_01175"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIC41681.1"
                     /translation="MKIATWNVNSINARIEHLTSFIKTDQSDIYLIQELKCTEDNFPY
                     DEIKYTGYNVYVNGQKAWNGVAILSKNPLKILNNKIPTFIDDKNARIIETEIKLSKLK
                     QSVKLFNIYLPNGNPIETDKFDYKIKWMKKFNDYILDLYNKNRPIIIGGDFNVIPTDE
                     DVYSPENYKNDACAHPRTREQYRILINSGFTDTVKFFIKGRTNWTFWGYRGGGWQKGN
                     GLRIDHFLTSPEITDLIQSVKIDRNPRGWEKASDHTPVILEIND"
     gene            complement(2817..>3009)
                     /locus_tag="EYO86_01180"
     CDS             complement(2817..>3009)
                     /locus_tag="EYO86_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HIC41682.1"
                     /translation="DNEVNSDDILISRSKIEILIDNTSLNFIKGSTIDYVNELIGSSF
                     KIKNPQASSSCGCGTSFSI"
BASE COUNT         1025 a          444 c          553 g          987 t
ORIGIN      
        1 tcttttaatg ctattttttc aaaatcagat agttctgcct atattgttat ttctaattca
       61 acactaatag ttcccttaga gggagttatt gataccaacg aagaaattgg caaactaaat
      121 ttaaaaaaga aaaatgaaac ttctgaatta attaatttac aaaataaatt aaataatcct
      181 cagttcattg ctaaagcgcc aaaaaatatt atagaacaat ttaaaatgca gtcaaaagaa
      241 attaaatctt ctattgaaaa aattgatcaa ataataaata ctataaaata gttatgggtg
      301 ataaaggatc aaatattaaa tcaaaattac caagtaggca cgtgagtgta ggtcctaaaa
      361 gtgctcctca tagatccatg tactatgcca tgggcttgac agaagaggaa atttatcaac
      421 cttttattgg agttgcaact acttggaatg agtccgctcc ttgcaatata accctaggaa
      481 ggcaagctca ggctgtaaaa attggggtaa aggaaaaatt aggaacgccc agagaattta
      541 caactattac ggttactgat ggaattgcaa tgggtcatga agcgatgaaa gcctctttag
      601 taagccgtga agtgattgct gatagtgttg aactttcagt tagaggtcat tgttatgatg
      661 gaatagtagg gttagcagga tgtgataaat cattgcctgg gttaatgatg gcaatggttc
      721 gcttaaatat tccctcagta ttcatttatg gtggttctat acttcctgga aagtataaag
      781 gcaaagattt aactataatt gatgtttttg aagctgtagg aaagcatgct cttggaacaa
      841 ttgatgacaa tgaattaaaa gcaattgagc aagttgcttg tccatctgca ggttcttgtg
      901 gaggacaatt tacggcaaat actatggcat gtatttctga agctttggga ttagcactga
      961 ctaattcaac tatgacacca gcaacttacg aagaaagaga taattatggt attgagagtg
     1021 gtagagcagt aatgaattta attgaaaaaa atattaggcc gagagatatt gtaacaattg
     1081 agtctttaaa aaatgcggct gtagtagttg ctgcaacggg aggatctaca aatgcagctt
     1141 tgcatttacc agctattgca aatgaggctg gcatcaaatt tacactgaga gatgttgtag
     1201 aaatttacca aaaaactcct tacattggag acatgtcccc aggaggaaaa tatgttgcta
     1261 aagatttgta tgatgtgggt ggagttcctg tagtaattaa atcactgctt gatggtggtt
     1321 atattaatgg agattgttta acagttacag gacaaacaat tgctgaaaat cataaagata
     1381 ccatttttcc tattaatcaa gatgttgttt acccttgtaa taaacccatc agtaatcata
     1441 gttccgtcgt tggcttatgg ggaaatttgg ctgaagatgg ttctatttca aaaattgctg
     1501 gattatccaa cttgcatttt aaaggcaaag caaaatgttt tgactgcgag gaagatgctt
     1561 taagtgctgt tttaaaaaat gaaattaatg caggtgatgc tgtcattata aggtatgaag
     1621 gtcccaaagg tggtccaggt atgcgtgaaa tgctatcaac tacagctgct atttatggtc
     1681 agggattagg tgaatctgta gctttaatta ctgacggaag attttctggt ggaacaagag
     1741 gattttgtat aggccatgta gggccagaag ctgcagaagg aggaatgatt ggtctgttga
     1801 aagatggaga tgaaattatc attgatgcaa taaaaggaac tatcaatgcc acactttctg
     1861 aagaagaaat tgaaaagaga aaaaaagaat ggaaaccacg tcaaactaat cacaattctg
     1921 gttcaatttg gaaatacgca cagactgttg gtcccgctta caaaggagca gtaacacagc
     1981 caggtgccaa aaatgaaacg catacttatg ctgatattta aattctaatc atttatttct
     2041 aaaatcaccg gagtatgatc cgaagctttt tcccaacctc ttggattcct atctattttt
     2101 actgattgaa ttaagtctgt tatttccggt gatgttaaaa aatgatcaat cctaaggcca
     2161 ttaccttttt gccaaccacc gcctctataa ccccagaatg tccaattagt tcttccttta
     2221 ataaaaaatt taacagtatc ggtaaatcct gaatttatta atattctata ttgttcacgc
     2281 gttcttggat gtgcgcatgc atcgtttttg taattttctg gagaataaac atcttcatct
     2341 gttggtatta cattaaagtc tcctccaata ataattggcc tgtttttatt gtaaagatct
     2401 agaatataat cattaaattt tttcatccat ttaattttat aatcaaattt gtctgtttca
     2461 ataggatttc cattaggcaa atatatatta aataatttaa cagattgttt aagtttagat
     2521 aatttaattt cagtttctat tatacgtgca tttttgtcgt caataaaagt tggaatttta
     2581 ttattcagaa tttttaaagg atttttactt aatattgcaa ctccattcca ggctttttga
     2641 ccatttacat aaacattgta tccggtatat ttaatttcat cataaggaaa attgtcttca
     2701 gtgcatttta actcctgaat aagataaata tctgattgat cagttttaat gaaactagtt
     2761 agatgttcta ttcttgcatt aatagaatta acattccatg ttgcaatttt cattaattaa
     2821 attgaaaagg aagtcccgca accacaggaa gaggaagctt gaggattttt tattttaaaa
     2881 gaggatccaa ttaactcatt tacataatca attgtagaac ccttaataaa attcaacgaa
     2941 gtattatcaa taagaatttc aattttcgat ctcgatatga gaatatcatc tgaatttact
     3001 tcattatca
//
LOCUS       DTVV01000097            2761 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1264494, whole genome shotgun sequence.
ACCESSION   DTVV01000097 DTVV01000000
VERSION     DTVV01000097.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1264494"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="EYO86_01185"
     CDS             complement(<1..840)
                     /locus_tag="EYO86_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC41683.1"
                     /translation="MHKKPIKYSVFSLFKNALSYHENWQPVWRSPAPKTEYDAIIIGG
                     GGHGLTTAYYLAKNHGLTKIAVVEKGWIGGGNTGRNTTIIRSNYLWDQSARLYDHALK
                     LWEGMSQELNFNVMFSQRGVMNVAHNLHDVRELKRRWHANRLNDIDCEWLNTKQVKEF
                     CPIINTSPNIRYPVLGATLQRRAGTARHDAVAWGYARGADAMGVDIIQNCEVTGINVQ
                     NGNVTGIETTKGRINSNRIGVVAAGHSSVIANMVGIRLPIESRPLQALVSEPVKPILD
                     TVVM"
     gene            complement(873..2534)
                     /locus_tag="EYO86_01190"
     CDS             complement(873..2534)
                     /locus_tag="EYO86_01190"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIC41684.1"
                     /translation="MSDIEIARKANKKNIKEIAIKLNLEENDLRPYGHYIAKIDNNST
                     QRLPKKESKLILVTAISPTPAGEGKTTTSVGLSDGLCKIGKQSIVCLREPSLGPSFGM
                     KGGAAGGGYAQVVPMEHINLHFTGDFHAITSANNLLASLIDNHIYWGNKLGIDVRRVS
                     WKRCLDLNDRALREITVNLGGVANGYPRQDGFNITVASEVMAIFCLANDLKDLERRLG
                     NITVAYTREKKAIFAKDLKAEGPMTVLLKQALMPNLVQTLENNPAIIHGGPFANIAHG
                     CNSVIATKTSLQLSDYVVTEAGFGADLGAEKFFDIKCRKSGLTPSAVVLVATIRALKM
                     HGGVAKANLSNENVEAVKSGISNLKRHVSNIKKFGLPVVIAINHFVADTENEMQALQE
                     ECKSIGVEAVPCYHWAKGSEGTVELAKKVVEIANGINTFKYLYEDKISLLEKIKTIAT
                     EIYYADDVIADSKIRDQLKQFEENGYGDFPICIAKTQYSFSTDPTLKGAPSNHSIPIR
                     EVRLSSGAEFIVVVCGEIMTMPGLPSVPAANSIFLNDEGEIEGLF"
BASE COUNT          844 a          545 c          433 g          939 t
ORIGIN      
        1 cattacaact gtatcgagaa ttggtttaac tggttctgaa acaagagctt gcaaaggcct
       61 gctttctata ggcagtctaa taccgaccat atttgcaata acgcttgaat gacctgcggc
      121 tacgacacct atacgatttg aatttatccg accttttgtt gtttcaattc ctgtgacatt
      181 tccattttga acattaattc ctgtaacttc gcagttttga atgatatcga cacccattgc
      241 atccgcgcct cttgcataac cccatgcaac ggcgtcatgt ctagcggttc cagcccgtcg
      301 ttgaagagta gctcctagaa ctggatagcg aatgtttgga gatgtattaa ttataggaca
      361 gaattccttt acttgttttg tatttaacca ttcgcagtca atatcattca gtcgatttgc
      421 gtgccaacgt ctttttagtt cacgaacatc gtgaaggtta tgagcaacat tcatcacacc
      481 acgttgcgaa aacattacat tgaaatttaa ttcctgagac attccttccc aaagtttaag
      541 agcgtgatca tataatcttg cactttgatc ccataaatag tttgaccgaa taatggttgt
      601 gtttcttcct gtgttgcctc cgccaatcca acctttttca acaactgcta ttttcgtaag
      661 gccatgattt ttggcgagat agtaagcagt tgttaagcca tggcctccac cgcctatgat
      721 gatagcatca tattctgttt ttggcgcggg tgagcgccaa actggctgcc aattttcatg
      781 ataggataaa gcgtttttaa agagcgaaaa taccgaatat tttattggct tcttgtgcat
      841 atgttataga attatgaaga tctataacag ttttagaaaa gaccttcaat ttctccctcg
      901 tcattaagaa aaatagaatt tgccgcagga acgcttggca gcccaggcat ggtcattatt
      961 tctccacaca caactacaat aaattctgct ccacttgata aacgtacttc tctgattggt
     1021 atactgtgat tagaaggcgc tccttttaaa gttgggtctg tagagaaact atattgagtt
     1081 ttagcaatgc agataggaaa atccccatac ccattttctt caaactgttt taattgatca
     1141 cgtatttttg aatctgcaat aacatcatca gcataataaa tttctgttgc aatcgttttg
     1201 attttttcaa gtaaagaaat tttatcttcg tacagatatt taaaagtatt gataccattg
     1261 gcaatctcaa caactttttt tgcaagctca acagtacctt cactaccttt tgcccagtga
     1321 taacaaggaa cagcttcaac accaatactt ttacattctt cttgaagtgc ttgcatttca
     1381 ttttctgtgt cagcaacaaa atgattgata gcaatcacca caggaagacc aaatttttta
     1441 atattagaaa catgacgttt taaattagat ataccagatt ttactgcctc tacattttcg
     1501 ttactcaaat ttgcttttgc aacaccccca tgcattttaa gagcacgaat ggtagcaact
     1561 aatacaacag cgcttggtgt aagaccagat tttcgacatt taatatcaaa aaatttttct
     1621 gccccaaggt ctgctccaaa accagcttca gtaactacgt aatctgataa ttgaagactc
     1681 gtttttgttg caattacact gttacaaccg tgagcaatat ttgcaaacgg acctccatga
     1741 ataattgcgg gattattctc caaagtttgt actaaattag gcattaaagc ttgttttaat
     1801 aaaacagtca tagggccttc tgcttttaga tcttttgcaa atatagcttt cttttctctt
     1861 gtgtaggcaa cagtaatatt tccaagacgt ctttctaaat cttttaaatc attagctaaa
     1921 caaaaaatcg ccataacttc agaagcaaca gttatattaa aaccatcttg gcgaggatat
     1981 ccatttgcaa caccacctaa atttaccgta atttcccgga gtgctctatc atttaaatct
     2041 aaacatcttt tccaagatac acgacgaaca tcaataccaa gtttatttcc ccaataaata
     2101 tggttatcaa ttagagacgc taaaagatta ttcgcagatg taattgcatg aaaatcacca
     2161 gtaaaatgta aatttatatg ttccattggt acaacctgag catatccccc tcccgcagca
     2221 ccacctttca tgccaaagct agggccaaga cttggttcac gcaaacacac aatgctttgt
     2281 tttccaattt tacagagacc atcactgaga ccaacagaag ttgtagtttt accttctcct
     2341 gcaggagttg gtgaaatagc agtaactaag ataagttttg attctttttt agggagtctt
     2401 tgagttgaat tattatctat tttcgcaata taatgaccat aaggacgaag atcattttct
     2461 tctaaattta gttttatggc aatttcttta atattttttt tattagcttt acgtgcgatc
     2521 tcaatatcag acatatggaa ttttcctcaa atttttattt cttctataga tgaattttgt
     2581 ttgaaaataa tattaatttc actattggta ataaaaaaaa agtggacaat ttaatttttt
     2641 atttccctat aattttaaat tcatttttac tattaatttc taaattagaa ttaataacgt
     2701 catataacca ttcagaggtt gttttgattc ctccaaaggc atagaaatgt aattttgcta
     2761 a
//
LOCUS       DTVV01000098            1575 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1265027, whole genome shotgun sequence.
ACCESSION   DTVV01000098 DTVV01000000
VERSION     DTVV01000098.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1265027"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            78..343
                     /locus_tag="EYO86_01195"
                     /pseudo
     CDS             78..343
                     /locus_tag="EYO86_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511580.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            347..1375
                     /locus_tag="EYO86_01200"
     CDS             347..1375
                     /locus_tag="EYO86_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIC41685.1"
                     /translation="MKKILVLGAGAMGSAFTVPCVDNNNDVVLVGSHLEDDAIDNIKK
                     ENNFHKILNCKLPKNLRVEKFKNFQDEIKTKPDLIVVGVNSKGIEWAGKEISKNYNSS
                     TSILLLTKGLSVINNKFETLAEKFKLILANNNVTESSISAVGGPCLASGLVNRIKSSV
                     VLANENKDVVKNIENIISTDYYSTEFSDDLLGVEICAAIKNIYSMIIGASEGLSSSNV
                     NKDIKSKYYLNTAGSLMYKAISEMVYFTKSLKGREESVYGLSGLGDLYVSSAGGRNSK
                     MGKYLGEGYDYKTAKEKFMKNETIEGAELALEIGTKVLKDFDRKKIPLMISLIESIIE
                     NKKLKINW"
BASE COUNT          597 a          175 c          303 g          500 t
ORIGIN      
        1 aaaatgcata tgcgggatgg gaatcggttg agaaagctcg tgaaaaaagc ttttttgatt
       61 ttgaatttat tacctggatg gcttggacat ggcaaactgc agcttttttt atttttattg
      121 ctctatgtct tgcaggaatg attttatggg aaaaaaagta ctaggcggaa caccaagaag
      181 gggtatattg agtttagata ctacaagagg tgatagatta tttatttctt tgttaagcgc
      241 cgcatatata cacttagctt ggttaggact tgtagatgca agtctatggt ttgctactgt
      301 tatttcagcc ttattctcta taatagtttt tagatacgtg taaaatatga aaaaaatttt
      361 agtattaggt gcaggcgcaa tgggttctgc tttcacagta ccctgcgtag ataataacaa
      421 tgatgtagtt ttagtaggct ctcatttaga agatgatgcc attgataata taaaaaaaga
      481 aaacaatttt cataaaattc ttaattgtaa attgccaaaa aatttaagag ttgaaaagtt
      541 taaaaatttt caagatgaaa ttaaaacaaa accagattta attgttgtgg gagttaattc
      601 aaaaggaatt gagtgggcag gaaaagaaat atctaaaaat tataactcat caacttctat
      661 tcttttactt actaaaggtt tgagtgtaat aaataataaa tttgaaacac tagctgaaaa
      721 atttaaatta attcttgcaa acaataatgt aacagaatca agtatttcag cggttggcgg
      781 cccgtgctta gcatctggat tagttaatcg aattaaaagt agcgtagtat tagctaatga
      841 aaataaagat gtagtaaaaa atatagaaaa tataatttct acagattatt acagtactga
      901 attttctgat gatttattgg gtgtagaaat ttgtgctgca atcaaaaata tttattctat
      961 gattattggt gcctcagaag ggttaagcag cagcaatgtt aataaagata ttaaaagtaa
     1021 atactattta aatacagctg gatctttaat gtataaagct atctcagaaa tggtgtattt
     1081 taccaagtca ttaaaaggta gagaggaaag tgtttatggg ttatcaggat taggagattt
     1141 atatgtaagt tctgctggag gaaggaatag taaaatggga aaatatctag gcgaaggcta
     1201 tgattacaaa actgcaaaag aaaaatttat gaaaaatgaa actatagaag gtgcagaatt
     1261 agctttagaa attggcacaa aagtattaaa agattttgat agaaaaaaaa ttcctttaat
     1321 gattagttta atagaatcca ttattgaaaa taaaaaatta aaaattaact ggtaatatga
     1381 aatcaaatta gaattaccca acaaatattt gggtaggaga acatagagtt gatgatttac
     1441 atgaaccaac caccctaaaa cctgagacgg gatacattaa aaaatggtac cttaaatcag
     1501 gaaaggaaaa atataatgaa gtgggctctt gtagtttatt ttatgactgc tagtggctgg
     1561 caatctgcgg agaca
//
LOCUS       DTVV01000099            2378 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1270738, whole genome shotgun sequence.
ACCESSION   DTVV01000099 DTVV01000000
VERSION     DTVV01000099.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1270738"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(322..1527)
                     /locus_tag="EYO86_01205"
     CDS             complement(322..1527)
                     /locus_tag="EYO86_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarL family transcriptional regulator"
                     /protein_id="HIC41686.1"
                     /translation="MTVNTDTERVPYGLAVHDHEEENAVLEIIRNHKTIMGEKVKQFE
                     NEIAVLFGKKFGVMVNSGSSANLLTYEILKMPENSEVITPILTFSTTLSPIIKNRLLP
                     VFVDVEPETYIVNIDQIEEAITKKTKALMIPSLLGNVPDLARLRKLADDNNLIFIEDS
                     ADTLGATFDGKPTGVFSDFSTTSFYGSHIITAAGEGGMVCCNNKKWEEKGRIIRGWGR
                     TSALNESEKLEERFGIELDKIPYDSKFIFEEIGYNFLPTEISAAFGFVQLKKLKKFSE
                     IRQENFDNLYSFFSNNKFLKVPKQLSQVKTSWLAFPLTITSNAPFSRMELVKYLETNN
                     IQTRPIFTGNVLRQPAFKNINYKNLENDFHVSNNIMKNSFLIGCNHGLTSKHIEKIKN
                     IFTKFLKNF"
     gene            complement(1540..>2378)
                     /gene="rfbG"
                     /locus_tag="EYO86_01210"
     CDS             complement(1540..>2378)
                     /gene="rfbG"
                     /locus_tag="EYO86_01210"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HIC41687.1"
                     /translation="HLGAQALVRKSYDDPLETFETNIMGTANLLESIRKCTSVKSTVI
                     MTSDKCYENKKMSRPHVEDDPMGGFDPYSASKGATELITSAYRNSFFKNNSNLSNIAT
                     ARVGNVIGGGDWADDRIIPDLIKGIIKNKNPVLRNPNSKRPWQYVLEPLSSILWLAAK
                     MYQEPTKFDEAWNFGPTSSSLTVRELSNAILKEWNTNLMCEIQNNSDDMPESDILLLD
                     SSKAEKNLGWKNILSIEETIFETVSWYKKNKNDMEEIKQFSLNQIENYVTKAKQRNTI
                     WA"
BASE COUNT          751 a          400 c          337 g          890 t
ORIGIN      
        1 gcatacggaa ttaaatctat tcgagtcaat gaaaccaata acttagatga agttatagag
       61 gaggtcttaa atcatgatgg gccagttata tgtgatgtaa ttgttcccag agagcaagaa
      121 gttattccta cagttgcttc aagggttaac aaggatggta gtatgtcatc aagaccactt
      181 gaagatatgt atccgttttt agatagaaaa gaatacaaag ataatttatt cattagtgaa
      241 attgattagc aagttgcatg atatgattta aaccgaacaa ttatccatcg atagtgaaaa
      301 aattatcacc atattgaaaa actaaaaatt tttcagaaat tttgtaaaaa tatttttaat
      361 tttttctata tgcttactag ttaatccatg attacagcca attaaaaatg aatttttcat
      421 tatattattc gaaacatgaa agtcattttc aagattttta taattaatat ttttaaatgc
      481 cggttgtcgc aatacatttc ctgtaaatat tggtctagtt tgaatattat tagtttctaa
      541 atatttcact aattccattc tagaaaaagg tgcgttagag gtaatagtta atggaaacgc
      601 taaccatgat gtttttactt gtgataattg tttaggaact ttgaggaatt tattattaga
      661 aaaaaaggaa tacaaattat caaaattctc ttgccgtatt tctgaaaatt tttttagttt
      721 ttttaattga acaaatccaa acgctgcact aatttcagtt ggcaaaaaat tatatccaat
      781 ttcttcaaaa ataaattttg agtcgtaagg aattttatct aactctatac cgaatctctc
      841 ttcaagtttt tctgattcat taagcgcaga tgttctaccc catccacgta ttattctacc
      901 tttttcttcc cattttttat tgttacagca aaccattcca ccttctcccg cggcagtaat
      961 tatatgtgaa ccgtaaaaac ttgttgtaga aaaatctgaa aatacaccag ttggcttccc
     1021 atcgaaagtt gctcctaaag tgtctgccga atcttcgata aaaattagat tgttatcgtc
     1081 tgccagtttt cttaatcttg ccagatctgg tacattacct aacaatgatg gaatcattag
     1141 tgcttttgtt tttttagtaa ttgcttcttc aatctgatca atatttacga tataagtttc
     1201 tggttctaca tcaacaaata cgggtaataa tctatttttt ataattggag ataatgtagt
     1261 agaaaaagtt agaattggag taatgacttc agaattttca ggcatcttca atatttcata
     1321 tgtcaacaaa tttgcagacg aaccagaatt taccataaca ccaaattttt ttccaaataa
     1381 caccgcaatc tcattttcaa attgttttac tttttctccc atgatggttt tgtgatttct
     1441 tattatttcc agaactgcat tttcttcttc atgatcatga acagctaaac catatggcac
     1501 ccgttctgtg tctgtattta cagtcattac gatttcattt taggcccaga ttgtatttct
     1561 ttgtttagct ttggtaacat aattttcaat ttgatttaat gaaaattgtt taatttcttc
     1621 catatcattt ttgttctttt tataccaaga aacagtttca aaaattgtct cttcaataga
     1681 tagtatattt ttccatccca aatttttttc cgcttttgat gaatctagta ataaaatatc
     1741 tgattcaggc atatcgtccg agttattttg tatctcacac attagatttg tattccattc
     1801 tttgagtata gcattagata gttctctaac tgttaatgaa ctactagtag gaccaaaatt
     1861 ccacgcctca tcaaattttg tcggttcttg atacattttt gccgccaacc acaaaatact
     1921 agagagtggt tccaaaacat actgccacgg tctttttgaa ttaggatttc taagtacagg
     1981 atttttattt ttgattatac cttttatcaa atcaggaatt attctgtcat ctgcccaatc
     2041 accaccacca attacatttc ccacacgcgc agttgcaata ttcgataagt tggagttatt
     2101 tttgaaaaat gaatttctat atgctgatgt aattaattca gtagcacctt tacttgcact
     2161 atatgggtca aatccaccca taggatcgtc ttcgacatga ggcctactca tttttttatt
     2221 ttcatagcat ttatcgctag tcatgattac agtagattta acacttgtac acttacgaat
     2281 tgattctaaa agatttgcag tacccataat gtttgtttca aatgtttcta atggatcatc
     2341 atacgacttt cttacaagtg cttgcgctcc aagatgaa
//
LOCUS       DTVV01000100            1072 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1273914, whole genome shotgun sequence.
ACCESSION   DTVV01000100 DTVV01000000
VERSION     DTVV01000100.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1273914"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1072
                     /locus_tag="EYO86_01215"
     CDS             <1..>1072
                     /locus_tag="EYO86_01215"
                     /inference="COORDINATES: protein motif:HMM:PF04898.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIC41688.1"
                     /translation="LAVLSENYKGLHHFFRQNFSQVTNPPIDSLREKVVMSLRTKIGN
                     LSNILDEDEYQCNHLQLSSPVLSINQFKTMRQYMKNTVKIIDTTMDLDEPNNSFEKTL
                     EKLNLESEQAVREGYVHIILTDKNLSKTRVALPMILVTSSVHHHLIKKNLRTFISLNV
                     QSAECLDVQYYSVLIGVGATSVSAYMAQQAIAERHKKGLFKNLSYEQSVDHYIASIKN
                     GLLKVMSKMGISVISSYRGGCNFEAIGLSRNLMSKYFPSMSSKISGMGLIGLEKRSLQ
                     AHHKAYEENLITLPIGGFYRYRHGGEKHAFESKSIHMLQTAVGSDNYQLYKKYTKLLG
                     ETQPINIRDLLSFRSFKTKANIE"
BASE COUNT          395 a          153 c          182 g          342 t
ORIGIN      
        1 actagctgtc ttgtcagaaa attataaagg tctccatcat ttttttaggc aaaattttag
       61 tcaagtaact aatccaccaa tagactcact gagagaaaaa gtagtgatga gtcttcgaac
      121 taagataggc aatcttagca atattcttga tgaagatgaa taccagtgca atcatcttca
      181 attgagttct ccagtattat caataaatca attcaaaaca atgcgacagt atatgaaaaa
      241 tactgtcaaa attatcgata ctacaatgga tcttgatgaa cctaataata gttttgaaaa
      301 aacacttgaa aaattaaatc ttgaatcgga acaagccgta cgtgaagggt atgtacatat
      361 tattttaact gataaaaatt tgtctaaaac tagagttgcc ttaccaatga ttttagtgac
      421 tagttctgtt catcatcatt taataaaaaa aaatcttcgc acattcattt cattaaatgt
      481 tcaatctgct gaatgtcttg atgttcaata ttactctgta ttaataggag tgggggcaac
      541 aagtgtgagt gcttatatgg ctcagcaagc tattgctgaa agacacaaaa aaggtctttt
      601 taaaaactta agttatgaac agagtgttga tcattatatt gcttcaataa aaaacggatt
      661 actaaaagta atgagtaaaa tgggaatttc agtaattagc tcatatagag gaggatgtaa
      721 ctttgaagct attggactga gtcggaattt gatgagtaaa tattttcctt ctatgagctc
      781 aaaaatatct ggcatgggtc ttattggctt agaaaaaaga tctcttcaag cgcatcacaa
      841 agcttatgaa gagaatttga ttactttacc tattggtggt ttttatcgct atagacatgg
      901 aggcgaaaaa catgcttttg aatcaaaatc aattcatatg cttcagactg cagtgggaag
      961 tgataattat caactttata aaaaatacac taaattatta ggcgaaacac agccaatcaa
     1021 tattagggat ttattaagtt ttagatcttt taaaacaaaa gctaatattg aa
//
LOCUS       DTVV01000101            2885 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_127446, whole genome shotgun sequence.
ACCESSION   DTVV01000101 DTVV01000000
VERSION     DTVV01000101.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_127446"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="EYO86_01220"
     CDS             <1..368
                     /locus_tag="EYO86_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41689.1"
                     /translation="CQYRLYFTWSEQIRAISFTVTFDIKFPQSKYESAHELLALINEK
                     LWIGHFDITKKNGIPAYRHTVLSLPENEMLQHQLEDLVDIAIYECEKYYPAFQLVLFD
                     DSLPSNALSVSTFDTIGSA"
     gene            407..1216
                     /gene="proC"
                     /locus_tag="EYO86_01225"
     CDS             407..1216
                     /gene="proC"
                     /locus_tag="EYO86_01225"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIC41690.1"
                     /translation="MNNILIIGCGHMGSALLTAWSSKTNNHFFVVDPAQYKNINKRLN
                     KKIHAFKTLNQVRGFLLFDIIIFAVKPQIAKRVLEKFVSLQFKKSAVFISIIAGKKIN
                     FFNQFLPLRNQFIRVMPNMPAIIDQGMSCMVSNRYVSRTNKNKIELLFSKVGLTLWLK
                     KEIEINKVTAISGSGPGYVFLFIDAFEKAALQLGLGKKSTKKLIRQTVSGSVNLLLQE
                     KKSAEELANNIAIKGGTTEAALNILKKNNDFHNKLKKAVKAAFLRANALSK"
     gene            1222..1773
                     /locus_tag="EYO86_01230"
     CDS             1222..1773
                     /locus_tag="EYO86_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41691.1"
                     /translation="MRQELIKIAQVTLKILSKKSWNSLSISEVKQKSKIKIFDNEIKN
                     KHVLLRNINAYFDHDLSLSVRGIEQSNRKDMIFEIIMMRFDILQKNRKALQSIFNSFK
                     SKPQELIFLLPYLLDSMILMANYANISVRGLRGQLRLKGILIIYCSTFLIWMKDDSTS
                     LEKTMTSLDSNLNKAGSILKFFQ"
     gene            1778..2116
                     /locus_tag="EYO86_01235"
     CDS             1778..2116
                     /locus_tag="EYO86_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HIC41692.1"
                     /translation="MSLISYDDFSKVQICVGTIIEAAEHDTLKKPSIVLTIDFGEKIG
                     IKKSSAQLQANYDSSKLLNKQIAAVINFPPKQIGKFMSEVLVLGFPDENNEPILVTPD
                     FKIKNGGKLF"
     gene            complement(2117..>2885)
                     /locus_tag="EYO86_01240"
     CDS             complement(2117..>2885)
                     /locus_tag="EYO86_01240"
                     /inference="COORDINATES: protein motif:HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HIC41693.1"
                     /translation="QIQPLKRLAIIAETFGRGLDAPELKVSGSLEIRQTTNAFNQMRT
                     RIKRFLKQRTDMLAGVSHDLRTPLTRMKLQLSLMKDDDAKKELEYDIKEMTAMLNSYV
                     SFVRGETPETIANIQLNNLIKNICQNLDREKYEITETYSRKIDTSGRPLQIKRAIQNI
                     LDNARRYASKIQIDVSVNNDECFISISDNGPGIPEKNYEDVFKPFFTLDPSRNKMKGE
                     SGLGMTISRDIIRSHGGDIKLSPSSIGGLKTVINLPI"
BASE COUNT          979 a          410 c          471 g         1025 t
ORIGIN      
        1 ggtgtcagta tcggctttat tttacttggt ctgagcagat tagagctata agttttacag
       61 ttacttttga tatcaaattt ccgcaatcta aatatgagtc tgcacatgaa ttactagctc
      121 tcatcaatga aaaactatgg attggtcatt ttgatataac taaaaaaaat ggtattccgg
      181 catataggca cacagtctta tctttgccag aaaatgaaat gcttcaacat caattagaag
      241 atttggttga tattgccata tacgagtgtg aaaaatatta tcctgctttt caacttgttt
      301 tatttgatga tagtttgcct agtaatgctt taagtgtcag cacatttgac acaataggta
      361 gcgcctaata actttacatt tttgagttat attttattca ttatgtatga ataatatttt
      421 aataattggt tgtggacata tgggttcagc tttattaaca gcgtggtcat caaaaactaa
      481 taatcatttt tttgttgtag atccagctca gtataaaaat attaataaaa ggctcaataa
      541 aaagatccat gcttttaaga ctttgaatca agttagaggt ttcttattat ttgatattat
      601 tatttttgct gttaaacccc aaattgcaaa aagagtttta gaaaaatttg tatcacttca
      661 atttaaaaaa tcggcggttt ttataagtat tattgctggt aaaaaaatta atttttttaa
      721 tcaattttta cctttacgga accaatttat tcgtgtgatg ccgaacatgc ccgcaattat
      781 tgaccagggt atgtcttgta tggtttctaa tcgttacgta tctcgtacta ataaaaataa
      841 aattgagtta cttttctcca aagttggact gactttgtgg ttaaagaaag aaatagaaat
      901 taataaggtg accgctattt cagggagtgg tccaggatat gtttttttat ttatagatgc
      961 attcgagaaa gctgccctgc aattgggatt aggaaagaag agtacaaaga aattgattcg
     1021 tcaaactgtt tccggttcag ttaatttatt attgcaagaa aagaaaagtg cggaagaatt
     1081 ggcgaacaat atagcaatta aaggaggaac aacagaggcg gctttaaata tattaaaaaa
     1141 aaataacgat tttcataaca aattaaaaaa agctgtaaag gctgcttttt tgcgtgctaa
     1201 tgcactaagc aaataaatta aatgagacaa gaattaatta aaatagcaca agtaacacta
     1261 aaaatattat caaaaaaatc atggaattca ttgtcaatca gcgaagtaaa acaaaaatca
     1321 aaaatcaaaa tatttgataa tgagataaag aataaacatg tattattaag aaacattaat
     1381 gcttatttcg accatgacct ttcattgagt gtcagaggaa ttgaacaatc taatcggaaa
     1441 gacatgattt ttgaaataat aatgatgcgt ttcgatattt tgcaaaaaaa tcgaaaggcc
     1501 ttacagtcaa ttttcaactc ttttaagagc aaacctcaag aattgatttt tttattgccc
     1561 tatctattgg atagtatgat tttaatggca aattatgcca atatttcagt tagaggatta
     1621 agggggcaat tacggttaaa aggcatctta ataatttatt gctcaacttt tttaatttgg
     1681 atgaaagatg acagcacttc cttagaaaaa actatgacat ctctagatag caatttgaat
     1741 aaagctggaa gcattcttaa attttttcaa tgattccatg tctttgattt cctatgatga
     1801 tttctccaaa gttcagattt gtgtaggaac tattattgag gcagcagaac atgatactct
     1861 taaaaaacct tcaatagtct taactattga ttttggtgaa aaaattggaa tcaaaaaaag
     1921 ttcagcacaa ttacaggcta attatgattc aagtaaatta ttaaataagc aaattgcagc
     1981 tgttattaat tttccgccta agcaaattgg aaaatttatg tctgaagttt tggtattggg
     2041 atttcctgat gaaaacaatg aaccaatatt agtcacacct gattttaaga taaaaaatgg
     2101 cggaaagctt ttttaattat atgggaagat taattacagt ttttaaccca cctatcgagg
     2161 atggagaaag tttgatatct ccaccatgcg atcgaattat gtctcttgaa atagtcattc
     2221 ccaatccgct ttctcctttc attttgttac gtgaaggatc taaagtaaaa aaaggcttaa
     2281 aaacatcttc ataatttttt tcaggtatgc caggtccatt atctgaaatg gagataaaac
     2341 attcatcatt gtttacagaa acatctattt gtattttgga agcataacgt cttgcattat
     2401 ccaaaatatt ttgtattgca cgtttgattt gtaaaggtcg tccagatgta tcgatttttc
     2461 tgctatatgt ttctgttatt tcatattttt ctctatcaag attttggcaa atatttttga
     2521 ttaaattatt taattggata tttgctattg tctccggagt ttctcctcta acaaaactta
     2581 catagctatt taacattgct gtcatttctt taatgtcata ttctaattct ttttttgcat
     2641 catcatcttt cattaaggat aattgcagtt tcattcttgt aagaggtgtg cgtaaatcat
     2701 gactaacacc agctaacatg tcagtccgct gttttaagaa tcttttaatt cttgtgcgca
     2761 tttgattaaa agcatttgtt gtttgtctaa tttcaagaga tccagacact tttaattcgg
     2821 gtgcatctag tcctcgtcca aatgtttctg caatgatagc taatcgtttc aagggttgta
     2881 tttga
//
LOCUS       DTVV01000102            1892 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1275726, whole genome shotgun sequence.
ACCESSION   DTVV01000102 DTVV01000000
VERSION     DTVV01000102.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1275726"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="EYO86_01245"
     CDS             complement(<1..310)
                     /locus_tag="EYO86_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41694.1"
                     /translation="MTIIKLTAEHMKVKQENALDLFYSGIKAKATKDRWARILSRFLE
                     EVCEEIFEGDYKQRAQKFVDLTRESQEQATQLVISYVQLLKQRTELDRKDPSYLNPSS
                     L"
     gene            444..1247
                     /locus_tag="EYO86_01250"
     CDS             444..1247
                     /locus_tag="EYO86_01250"
                     /inference="COORDINATES: protein motif:HMM:PF13361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41695.1"
                     /translation="MDHIELTQLILEDSKYVEYLEQEEKNSKNPENLNRLENIKEFIE
                     SLKDFENLEGFLEHVGLVMENISNTNDESISLMTMHSAKGLEFDNVFLAGWEEGVFPS
                     MRSIDELGKKGLEEERRLAYVALTRARRKIHITYVNQNRYSYVSHDFNIPSRFIEELP
                     KNLVNINNSTYLSNNNFIDEFSQIENMDSSFITPGRKRLLSRIKKEEDDWDFNQDYQS
                     NSNATEGSRVFHQKFGYGKIIFIDGDKAEVDFEKSSQRKIFLKYLQFTA"
     gene            complement(1237..>1892)
                     /locus_tag="EYO86_01255"
     CDS             complement(1237..>1892)
                     /locus_tag="EYO86_01255"
                     /inference="COORDINATES: protein motif:HMM:PF00533.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIC41696.1"
                     /translation="GVKQIKQFYDLNFIKKIDDIFLINMHKNNILELDGWGDQSYNNL
                     IKSIDNSKKINLERFIFAIGIRYVGETISTLLAKEFLNIDNFINNSKNYERLINIDGL
                     GPKAIDSIVDYFSNTLNQKTILNLIKILTISNFRKPQSSSFFNNKSIVFTGSLLKLSR
                     DEAKHLARELGATITSTVSKRTDYVIVGDKPGSKAKKAMELKIPIMTEDDWLNKIKP"
BASE COUNT          647 a          261 c          305 g          679 t
ORIGIN      
        1 gaagtgaaga tgggtttagg tatgaaggat ctttcctgtc aagttctgta cgctgtttta
       61 gtaactgtac gtatgagatg acaagctgtg ttgcttgctc ttggctttct cttgtaaggt
      121 caacaaactt ttgtgcacgt tgtttgtaat ctccctcaaa gatttcctca caaacttctt
      181 ctaagaacct acttagaatt ctagcccacc tatcttttgt tgcctttgct ttgattccag
      241 aatagaatag gtctagtgca ttttcctgct ttaccttcat gtgttcagca gttagcttaa
      301 tgattgtcat gtttgatcat tctcctacac acagtgttac ttttcgaggt cagaattccg
      361 acattatttt gttgaatctc aaaaatgaac ctagatgagt atggatccga agggatgtgc
      421 atcattattg atgaaaaaaa gaaattgatc atattgagtt aactcagttg attttagaag
      481 attctaagta tgttgaatat ctagagcagg aagaaaaaaa ttcaaaaaat cctgagaacc
      541 taaatcgttt agagaatata aaggaattta ttgaaagtct aaaggacttc gaaaacttag
      601 aaggtttttt ggaacatgta ggtttagtga tggaaaatat ttcaaatact aacgatgagt
      661 caatatcatt gatgacaatg catagcgcta agggtctaga atttgacaat gtttttttag
      721 ctggatggga agaaggtgtc tttcccagta tgagatctat agatgagttg ggaaaaaaag
      781 gcttagaaga agaaagacgc ctagcttatg tagctttaac aagagcaaga agaaaaattc
      841 atataaccta tgttaatcaa aatagatatt catacgtatc acatgatttt aatatacctt
      901 ctagatttat tgaagaactt cctaaaaatc tagttaatat taataattcc acgtatttga
      961 gtaacaataa ttttatagat gagttttcac aaattgaaaa tatggattct agcttcatta
     1021 caccagggag aaaaaggtta ttgtctcgta ttaaaaaaga agaagatgat tgggatttta
     1081 atcaagatta tcaatcaaat tctaatgcaa cagaaggttc aagagtattt catcaaaagt
     1141 ttggctatgg aaaaattata tttatagatg gagataaggc tgaagtggat tttgaaaaat
     1201 cttctcaaag aaaaatattt ttaaaatatt tacaatttac ggcttaattt tattaagcca
     1261 atcatcttca gtcatgatag gtatttttaa ttccatagct tttttagctt tgctgccagg
     1321 cttatcacca acaataacat aatctgttct ttttgaaact gtgctggtta ttgtagcacc
     1381 taattctcga gctagatgtt tggcctcatc tcttgaaagc tttaataaac taccagtaaa
     1441 gacaatgctt ttattattaa aaaaactgct tgattgaggt ttccgaaaat tagaaattgt
     1501 caatattttt attaaattca aaattgtttt ttgatttaat gtattagaaa agtaatcaac
     1561 tatagaatct attgctttgg gtcctagacc atcaatattt atcaatcttt cataattttt
     1621 tgaattgtta ataaagttgt cgatattaag aaattcttta gcaagcaatg ttgatatagt
     1681 ttcacctaca tatctaatac ctattgcaaa aataaatctt tctaaattaa tcttttttga
     1741 attgtcaatg gattttatta gattattgta ggattgatca ccccaaccat ctaattctaa
     1801 aatattattt ttatgcatat taataagaaa aatatcatca atttttttaa taaaatttaa
     1861 atcataaaat tgttttattt gttttacccc aa
//
LOCUS       DTVV01000103            1255 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1277471, whole genome shotgun sequence.
ACCESSION   DTVV01000103 DTVV01000000
VERSION     DTVV01000103.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1277471"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="EYO86_01260"
     CDS             <1..498
                     /locus_tag="EYO86_01260"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HIC41697.1"
                     /translation="NLHSNFFFGLAIGGSLGCSKEEMHQVVEYTASKLKDKHPVHLLG
                     IGDPSDIWSLVRWGVDTFDCVSPTRLARHGAALKKTRLGKINIKNSKYREDLNPIDSK
                     CQCKTCANYSLSYIHHLLKSDELLGLQLITNHNIYFMNDLMLYIRKAINEDKLLEAEK
                     DWYQD"
     gene            complement(495..>1255)
                     /locus_tag="EYO86_01265"
     CDS             complement(495..>1255)
                     /locus_tag="EYO86_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HIC41698.1"
                     /translation="RQWEFNLEEGYLDTSRLARIIANPTHKLSFKKEKNIEFKDTVVT
                     LLIDNSGSMRGRPITVAALCSDILARTLERCLIKTEILGFTTKAWKGGQSREKWINDK
                     KPSKPGRLNDLRHIIYKAGDAPWRRSKKNLGLLLREGILKENVDGEALLWAYNRLRIR
                     QEKRKVLIVISDGAPVDDSTLSVNPGNYLEKNLRDTISDIENNSNIELVAIGIGHDVS
                     RYYSNAVTIMDVDQLGEVLLNELSNIFVTKKKYN"
BASE COUNT          387 a          224 c          201 g          443 t
ORIGIN      
        1 aatcttcatt caaatttttt ttttggtcta gctataggtg gcagtcttgg ctgttcaaaa
       61 gaagaaatgc atcaagttgt tgagtataca gcttcaaaac ttaaagataa acacccagtt
      121 catttacttg gaataggcga tccctcagat atatggagtt tggttcgttg gggggtcgat
      181 acctttgatt gtgttagtcc tacacgttta gcccggcacg gtgccgcatt aaaaaaaaca
      241 cgattgggta aaattaatat taaaaattca aaatatagag aagatttaaa tcctattgat
      301 agtaaatgcc aatgtaaaac atgtgccaac tattccttat cttatataca tcatttgctt
      361 aagtcggatg aattactagg attgcagtta ataactaatc ataacattta ttttatgaat
      421 gatttgatgc tttatattag aaaagcaatt aatgaagata aattgttaga agcagaaaaa
      481 gattggtatc aagattaatt atattttttt ttagtaacaa aaatattgga aagttcattc
      541 aataatactt ctcctagttg gtcaacatcc attattgtaa ccgcgttaga ataatatcta
      601 gatacatcat gaccaattcc tatggccact aattcaatat tgctattatt ctcaatatcg
      661 ctaattgtat cccgtaaatt tttttccaaa taatttccag ggttaaccga taacgttgag
      721 tcatcaacgg gtgctccatc actaataaca atcaatactt ttcttttttc ttgccgtatc
      781 cttaatctat tataagccca taacagcgct tccccatcca cattttcctt cagtatgcct
      841 tcacgcaata aaagtccgag gtttttttta gagcgtctcc atggagcatc acctgcttta
      901 tatatgatat ggcgtaaatc attaagtctt cctggtttac taggcttttt atcgtttatc
      961 catttttctc ttgattgacc ccccttccaa gcttttgtag taaagcctaa aatttcagtt
     1021 ttaatgaggc atcgttctag agttcttgct aagatatctg aacataaagc tgcaacagtt
     1081 ataggccttc cgcgcataga ccctgaatta tcaattaata aagtaacgac agtatcttta
     1141 aactcaatgt ttttttcttt tttaaacgat agtttgtgag ttgggttggc aatgatcctt
     1201 gctaagcgtg aagtatcaag atagccttct tcaagattga attcccattg tctgg
//
LOCUS       DTVV01000104            1040 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1278558, whole genome shotgun sequence.
ACCESSION   DTVV01000104 DTVV01000000
VERSION     DTVV01000104.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1278558"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(61..846)
                     /locus_tag="EYO86_01270"
     CDS             complement(61..846)
                     /locus_tag="EYO86_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HIC41699.1"
                     /translation="MKLNSANINVMVKACRKASKTVIRDFGEIENLQVSLKGPGDFVT
                     NCDKKVEKILIDELLKARPSYSILSEEIGEINNDDSFKWIIDPIDGTSNFLHGVPHFA
                     ISVGLEHNKEIICGIIYDPIKDEMFTAEKGNGSYMNNQRMRVSSRSKLEDCMIFTGGP
                     KRDAKNRELALKEYYKFSIKVLTPIRKLGSASLDMAYVAAGRCDGFLQRNLNYWDVAA
                     GIILVKEAGGFVTDFNGESEYIQNKTILATNAKINEEMIEVLK"
     gene            complement(847..>1040)
                     /locus_tag="EYO86_01275"
     CDS             complement(847..>1040)
                     /locus_tag="EYO86_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIC41700.1"
                     /translation="DLPSSVELKIETTDAAIKGQTASSSYKPATLENGIKIMVPPFIN
                     ADDKIILDTRTLEYVKKIK"
BASE COUNT          320 a          190 c          133 g          397 t
ORIGIN      
        1 cttttttcat aacggatagt ttattatagt aaaataaatt atttgacaat agtaaattaa
       61 ctatttcaat acctcaatca tttcttcatt aatttttgca ttagttgcta gtatagtttt
      121 attttgtatg tattcgcttt ctccattaaa atctgtaaca aatcctccag cttccttgac
      181 taaaataata cctgccgcga catcccaata atttaaattt ctctgcaaaa aaccatcaca
      241 tctccccgcg gctacatatg ccatatcgag cgaagcactt cctaatttcc tgataggagt
      301 aagtactttt attgaaaatt tataatattc ttttaaagct aattctctgt ttttggcatc
      361 acgtttaggt ccgcctgtaa aaatcataca atcttctagt tttgatctag atgaaacacg
      421 cattctctga ttgttcatat atgatccgtt acctttttcg gcagtaaaca tttcatcttt
      481 aatagggtca taaataattc cacatattat ttctttgtta tgttctaacc ctaccgatat
      541 tgcaaaatga ggtacgccat gaagaaaatt tgaagttcca tctatcggat caatgatcca
      601 tttaaaagaa tcatcattat ttatttcacc aatttcttca cttaaaatgg aataacttgg
      661 tcttgcttta agcaattcgt caataagaat tttttctact tttttgtcac aattagttac
      721 aaaatcacca ggacctttaa gagaaacctg aagattttct atttctccaa aatctctaat
      781 cacagttttt gaagcttttc tacaagcttt taccatgaca tttatatttg ccgagttgag
      841 tttcatttat ttaatttttt ttacatactc taaagttctt gtatctaaaa ttattttatc
      901 atcagcattg ataaagggag gaaccataat tttaatacca ttttccagag ttgctggttt
      961 gtaagatgaa gaagccgtct ggcctttaat cgcggcatct gtggtttcaa tttttaactc
     1021 tactgaagat ggtaaatcaa
//
LOCUS       DTVV01000105            1217 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1279959, whole genome shotgun sequence.
ACCESSION   DTVV01000105 DTVV01000000
VERSION     DTVV01000105.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1279959"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="EYO86_01280"
     CDS             complement(<1..824)
                     /locus_tag="EYO86_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC41701.1"
                     /translation="MLFNNQPYVGQKKASLSLRFGAAFGILYLNVPLFFIILYAFTTE
                     DKSFQFPPPGYTLKWFKIVLGREDIWNAVQLSLKIACLSTIIAIVLGTLAAAALYKRS
                     FFGRETITLLIILPIALPGIITGISLRSAYGFAGIPFSSLTIIIGHATFCMVVVYNNV
                     VARLRRSSPSLIEASMDLGANNFQTFRYVILPNLSTAILAGGMLAFALSFDEVIVTTF
                     TAGQQTTLPIWMLTELIRPRQRPVTNVVAVFIICITFVPILIAYYLTRDHDSADGAG"
     gene            complement(814..>1217)
                     /locus_tag="EYO86_01285"
     CDS             complement(814..>1217)
                     /locus_tag="EYO86_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003566682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41702.1"
                     /translation="SISYIGMFLVFTFIWLPFMIIPIQASLERVPNSLLDASSDLGAP
                     PRKTFYKIILPLALPGIAAGSIFTFSLTLGDYIIPYIIGTSKFFIGQAVYTHQGVAGN
                     VPLAAAFSIVPIAVMAIYISLAKRLGAFDAF"
BASE COUNT          463 a          208 c          195 g          351 t
ORIGIN      
        1 cctgcaccat cagcgctatc atgatctcta gtcaaataat aggcaataag tattggcaca
       61 aatgtaatac aaattataaa aacagcaaca acatttgtca ctggtcgttg ccttggtcga
      121 attaattctg ttaacatcca tataggtagt gttgtttgtt gacccgctgt aaatgttgta
      181 acaatcactt catcaaatga taaagcaaaa gccaacatcc ctcctgctag aatagcagtt
      241 gataaatttg gtaaaattac atatctgaag gtttgaaaat tattagctcc taaatccata
      301 gaagcctcaa ttaatgatgg tgaacttctt cttagtcttg caacaacatt attatagaca
      361 acaaccatac aaaaagtcgc atgacctatt ataattgtta atgagctaaa aggaatacct
      421 gcaaatccat aagcgctacg taaagaaatg cccgtaataa ttcctggtaa tgctattggt
      481 aaaataatta ataaggtaat agtttctctt ccaaaaaacg aacgtttata aagagctgca
      541 gctgcaagag ttccaagcac aattgcaata atagttgata gacaagctat ttttaaggaa
      601 agttgaacgg cattccaaat atcttctctt cctaaaacta tcttaaacca tttaagcgta
      661 taacctggtg gaggaaattg aaacgattta tcttcagttg taaatgcata aagaataata
      721 aagaataacg ggacgtttaa atataaaata ccaaatgctg ctccaaatct taaagaaaga
      781 ctagctttct tttgccctac ataaggttga ttattaaaaa gcatcaaagg cacctaatct
      841 tttagctaaa gatatataaa ttgccatcac agcaattggt acaatagaaa atgccgctgc
      901 taatggaaca ttaccagcta ctccttgatg tgtataaaca gcttgtccaa taaaaaattt
      961 ggatgtccct ataatataag gaataatata atcgccaagg gttaatgaaa aagtaaaaat
     1021 agaaccggct gcaattcctg gtaaagctaa tggtaaaatt attttataaa acgtttttct
     1081 aggaggggct cctaaatcac ttgacgcatc caataaacta ttaggaactc tttctaatga
     1141 agcttgtatg ggaataatca taaatggtaa ccagataaaa gtgaaaacaa gaaacatacc
     1201 aatatatgat atggata
//
LOCUS       DTVV01000106            1085 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1283358, whole genome shotgun sequence.
ACCESSION   DTVV01000106 DTVV01000000
VERSION     DTVV01000106.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1283358"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="EYO86_01290"
     CDS             complement(<1..135)
                     /locus_tag="EYO86_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HIC41703.1"
                     /translation="MHEIAKKDKLIYFKFLNSLPKKINKLYFGKLEGKFRLNNKSKKK
                     F"
     gene            complement(150..>1085)
                     /locus_tag="EYO86_01295"
     CDS             complement(150..>1085)
                     /locus_tag="EYO86_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC41704.1"
                     /translation="AQAYAILANDGRKVPMSLLRVDGLPEGVFNVLPGDGTTGKALCM
                     HNDVDCVAFTGSGEVGKKILGYSGQSNMKRVQLECGGKSPNIIFSDCENIDTIVKTSA
                     NAIFGNQGEVCSAASRLIVQDSIKDEFIEKVVNESKNMMPGDPWDPKSFMGAIVDKTQ
                     LDKIDGYVQEGQKEGAKVAIGGKVMLKNTGGYYYEPTVFDNVKNNMKIAQEEIFGPVL
                     SSITFKSTEEALKIANDTSYGLAAAVWTRDINKAHLMAKGIKAGSIFVNNYDEADLTV
                     PAGGYKQSGIGRDNSYHALDTYLQVKSTWIKLSDK"
BASE COUNT          318 a          223 c          147 g          397 t
ORIGIN      
        1 aaattttttt ttcgatttat tgtttagtct aaatttacct tctaattttc caaaatatag
       61 tttgttaatt ttttttggaa gagagtttag gaatttaaaa taaattaact tatctttttt
      121 agcgatttca tgcatgaata gaaataaatt tatttatcag ataatttaat ccaagttgac
      181 ttaacttgaa gataagtatc aagagcatga tatgaattat ctctacctat tcctgattgt
      241 ttgtatccac ctgcaggaac agttaaatct gcttcgtcat aattgttaac aaatatggat
      301 cccgctttaa ttcccttagc catcaggtgt gctttattaa tatctcttgt ccaaacagcg
      361 gctgctaaac catagcttgt gtcattagca atttttaatg cctcttcagt acttttaaaa
      421 gtaatacttg atagaactgg tccaaaaatt tcttcttgcg caatcttcat attatttttt
      481 acattgtcaa aaacagttgg ctcataataa taacctcctg tgtttttcaa cataacttta
      541 cctccgatag caacttttgc gccttctttt tgaccttctt gaacgtagcc atcaatttta
      601 tctaattgag ttttatcaac aatcgctccc ataaaagatt tgggatccca tggatctccg
      661 ggcatcatat ttttactttc attaacaact ttttcaataa attcatcttt aattgaatct
      721 tgaacaatta gtctcgatgc cgctgaacaa acctctcctt gatttccaaa aatagcattt
      781 gccgatgttt tgacaattgt gtcaatattt tcacaatcag aaaaaattat attgggagat
      841 tttcctccac actcaagttg aactcttttc atattacttt gtccagaata tcctaatatt
      901 ttttttccaa cttctcctga tccagtaaaa gctacacagt caacatcgtt atgcatgcat
      961 agtgcttttc ctgttgttcc atctccaggc aaaacattaa acactccttc gggcaaaccg
     1021 tccacccgca gcaaagacat cggcaccttg cgaccgtcgt tggccagaat cgcataggcc
     1081 tgcgc
//
LOCUS       DTVV01000107            1315 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1283506, whole genome shotgun sequence.
ACCESSION   DTVV01000107 DTVV01000000
VERSION     DTVV01000107.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1283506"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="EYO86_01300"
     CDS             complement(<1..1084)
                     /locus_tag="EYO86_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIC41705.1"
                     /translation="MNDIISQRLKNVKPSMTVAINVKANALRAKGQDVLVLAAGEPDF
                     DTPINICEAAVKAMNEGQTRYVPGKGTPALQNAIVDKFKRDNDLSYNLDEIMVGVGGK
                     HIIYNAMMATLNLNDEVIIPAPYWVSYPDIVILAEGKPVIVKCEASQNFKITPDQLEK
                     HITDKTKWLMLNSPSNPTGSVYSFEELKALSEVLIKYPKILVLTDDIYEKIIYDDNKF
                     FTIASVEPKIKDRVLTLNGVSKAYCMTGWRLGYCGGPKEIISGMNKIQSQSTTSTSSI
                     TMAAAVEALNGDQEFIKKHNKEFKKRRDLVVEKLINIEDLSCEIPPGAFYVYPCCEGI
                     IGKKTPEGNTIETDEDLMNFLLESEGI"
     gene            complement(1081..1299)
                     /locus_tag="EYO86_01305"
     CDS             complement(1081..1299)
                     /locus_tag="EYO86_01305"
                     /inference="COORDINATES: protein motif:HMM:PF01925.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41706.1"
                     /translation="MNNAKFFAGHSLGYVSLPIVIIVASVSIFTAPLGAKLSNQTNKR
                     TLKKFFATFLLLICVTLVIEQFYLNQIK"
BASE COUNT          416 a          232 c          182 g          485 t
ORIGIN      
        1 ctattccttc gctttctaaa agaaaattca tcaaatcttc atcagtttca attgtattac
       61 cctctggagt tttctttcca atgatacctt cgcaacatgg gtaaacgtaa aatgctccag
      121 gagggatttc acaagaaaga tcttctatat tgatcaattt ttcaactact aaatcacgtc
      181 tttttttaaa ttctttatta tgttttttta taaattcttg atcaccatta agcgcttcaa
      241 ctgcggcagc catagtaatt gatgatgttg aggtagtgct ttgagattga attttgttca
      301 ttccagaaat aatttctttt ggtccaccac aataacctag tcgccatcct gtcatacagt
      361 atgctttaga aactccattg agagttaaaa ctctatcttt tatttttggc tcaacagaag
      421 cgatagtaaa aaatttgtta tcatcataaa taattttttc ataaatatca tcagtaagaa
      481 ctaaaatttt tgggtatttt attaaaactt cacttaaagc ctttaattct tcaaatgaat
      541 aaacagaacc tgttggatta cttggactat ttaacattaa ccattttgtt ttatctgtaa
      601 tatgtttttc taattggtca ggtgttattt taaaattttg tgacgcttca catttgacaa
      661 ttacaggttt cccttcagct aaaataacta tatctggata actcacccaa taaggagctg
      721 gaattataac ttcatcattt agatttaatg tagccatcat agcattatag attatatgct
      781 ttccccctac cccgaccatt atttcatcta agttgtaaga caaatcatta tcacgtttaa
      841 atttatctac tattgcattt tgtaaagccg gtgttccttt gccaggaaca taacgagttt
      901 gtccttcatt cattgcttta actgctgctt cacaaatatt gataggtgta tcaaaatctg
      961 gctcacctgc tgctaacact aaaacatcct gaccttttgc gcgtaaagca ttagccttaa
     1021 catttattgc aactgtcatg gaaggcttaa cgttttttaa cctttgggat ataatgtcgt
     1081 tcatttgatt tggtttaaat aaaattgttc aattacaaga gttacacata tgagcaacaa
     1141 aaaagtagca aaaaattttt ttaatgttcg tttgtttgtt tgatttgaaa gtttagctcc
     1201 taaaggagca gtaaatatac ttactgaagc tactattata acaataggta aacttacata
     1261 acctaaggaa tgtcctgcaa aaaattttgc attattcaaa tttaagccaa actcc
//
LOCUS       DTVV01000108            2752 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1287106, whole genome shotgun sequence.
ACCESSION   DTVV01000108 DTVV01000000
VERSION     DTVV01000108.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1287106"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="EYO86_01310"
     CDS             complement(<1..602)
                     /locus_tag="EYO86_01310"
                     /inference="COORDINATES: protein motif:HMM:PF05343.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HIC41707.1"
                     /translation="MNKNLIKLLKDLCLIPGLSGYEDKVRSYIRNELSNAGISSKSDV
                     LGNLIVTLEGTKDKPSIMLFTHMDQLGFIVKKIESNGLIRFERLGGVPEKALASQEIV
                     ITANDGKFYEGIISNKSHHATESEEKYIVIPYKDLFIDIGFESEKEVDGIGIKIGSPI
                     TYAPKAKQIGANKFAGTAVDDRAGCAVVIETAKKTQGLKA"
     gene            complement(614..1416)
                     /locus_tag="EYO86_01315"
                     /pseudo
     CDS             complement(614..1416)
                     /locus_tag="EYO86_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005794972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
     gene            complement(1515..1967)
                     /locus_tag="EYO86_01320"
     CDS             complement(1515..1967)
                     /locus_tag="EYO86_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter"
                     /protein_id="HIC41708.1"
                     /translation="MLINVDPLLSPDILSTLRSMGHGDRLVLSDANFPSYSHNSTLHR
                     MDGLNINQAAKAILSVFPLDSFIAEPVLRMEIDGKPDEINEVHQDLINTVKNISGNNW
                     KVGSIERQKFYAEAKKAFAVITTSDTRPYGCFIFIKGVLKPDGSVWIV"
     gene            2312..>2752
                     /locus_tag="EYO86_01325"
     CDS             2312..>2752
                     /locus_tag="EYO86_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stearoyl-CoA 9-desaturase"
                     /protein_id="HIC41709.1"
                     /translation="MESVDLSKWYRPSVDQEEFKKLCKKSDWPGFKHMIIFFGSLFFF
                     GYLAFLTWGTWWSVLFFLIYGNIYCTCDAIWHETGHKTAFKSKFWNEFFYQIASYMNN
                     FEPVRWRWSHFKHHSHTASTKDPHDFEIAISKPTDVLYFFSLFIP"
BASE COUNT          877 a          492 c          405 g          978 t
ORIGIN      
        1 tccgctttta gtccttgtgt tttttttgca gtttcaataa ctactgcaca accagcacga
       61 tcatctacag ctgttccagc gaacttattg gctcctattt gctttgcttt aggtgcataa
      121 gttatgggcg aaccaatttt tattccaatc ccatctactt ctttttcgct ttcaaagcct
      181 atatcgataa atagatcttt atagggaatt actatatatt tttcttcaga ttcagttgca
      241 tgatgtgatt tgtttgaaat tataccttca taaaatttac cgtcatttgc agttataaca
      301 atttcttgag aagctagtgc tttttctgga acgccgccta atctttcaaa tctaattaaa
      361 ccatttgact caattttttt cacaatgaaa cctagttggt ccatatgagt gaaaagcata
      421 attgagggtt tgtcttttgt gccttctaac gtaactataa gattgcccaa aacatcactt
      481 tttgatgaaa ttcctgcatt tgaaagttca tttctaatgt aggatcgtac tttatcttca
      541 tatccgctta aacctggtat caagcacaaa tctttaagta attttattaa gtttttattc
      601 atttatttga atatcatctt atagatttta ctttattcat aaaatccaat gcacgtttag
      661 gatcaatggg tttccaagta tcaccattta cttttaaaga tgaacctact attacgccgt
      721 cagcaatttt taatatattc gtaactgttt ccaattttac tccagtattt gcaagcacag
      781 gagtatcagg aagtactttt ttaacagact ctatatcatc tattttagca gcctcacctg
      841 taatttggcc tgaaacaaga actgcatctg gaatcgaaga aaacactaca ctgcgagatc
      901 tatctgccaa agatctttga tcaagagaat aagcaaattc agcgcttaca ttataaaaca
      961 ttagaagatc atttctgtct agactttttt tatagcgtaa tgctttagct gcatctggaa
     1021 tccataaacc catatctgag gtataaacac cactaaaaat ttctctcaca aattgcgcac
     1081 ctgttgcggc agctactgca attgtactta aggggtccca aagaacattt acaccaaagg
     1141 gtactttaat tttttctttt aaaaatccta tcacataaga catggtcgca gaactcgcaa
     1201 tatttgcttt tagttcataa ggacgatcat tttcattgcc aaataaaact gcatctacat
     1261 cagcatcttg caaagcttcc aaatctcttt gtgcactttc aataatataa tttaaaccta
     1321 gttttggatc atacaatggt gaaccaggta aggcaggcaa atggatcatt caataactgg
     1381 cttgtcattt ttaaaaattt ttttaaaatt tttcatttcg aaatactcta taacaaatat
     1441 taattttaaa aaaatatata aaaatctctt aaattattga aaaaaacaag aaatacaggt
     1501 aattttatct agatctaaac gatccaaaca ctgccatcgg gttttaggac gccttttata
     1561 aaaataaagc atccgtaagg cctagtatcg cttgtagtaa ttactgcaaa tgccttttta
     1621 gcttcagcat aaaatttctg tctttcaatt gagccaactt tccagttatt tcctgaaata
     1681 ttttttactg tattgattaa gtcttgatgc acttcattaa tttcgtctgg ttttccatca
     1741 atttccattc ttagaacagg ttctgctata aaactatcaa gaggaaaaac tgataaaata
     1801 gctttggcag cttgatttat gtttaagcca tccatcctat gcaatgttga gttatgtgaa
     1861 tatgaaggaa agttggcatc acttaatacc aacctatctc catgtcccat agatcgaaga
     1921 gttgataaaa tatcaggact taaaagtgga tcaacattaa taagcatatt tttttactac
     1981 catagtattc tccaaaagat aaccaaatat ttttaatcta ggaactcaaa aataaaaaat
     2041 ttatttagaa atttccttga tgtattttta ccatctattc tttttgcaag gcaatacttt
     2101 ctttatcgca gcagcacagt aacttttaat attgctcgga caatggtttc ttggtttctt
     2161 tacaaccaca agatatgttg ggacgtaaaa aattaatttt ctaaacacga agtgttgtat
     2221 aaatgttgca aatagttctt actattaggt gatttggggc acacgaaagt taacatttta
     2281 ctgtttttga aaaaatgcta gaatatcacc tatggaaagt gttgatctaa gtaaatggta
     2341 tagaccctca gttgaccaag aagagtttaa aaaactttgc aagaaatctg attggcctgg
     2401 ttttaagcat atgataattt tctttggatc attattcttc tttggctatc ttgctttttt
     2461 gacatgggga acttggtgga gtgttttatt ttttctcatt tatggtaaca tatactgcac
     2521 atgtgatgcc atatggcacg aaacaggaca taaaacggct tttaaatcaa agttttggaa
     2581 tgaattcttt tatcaaatcg ctagttacat gaataacttt gaaccagtac gttggcgctg
     2641 gtcacacttt aaacatcata gtcatacagc atctacaaaa gatccgcacg attttgaaat
     2701 tgcaataagc aaaccaacgg atgtacttta tttcttctca ctttttattc ca
//
LOCUS       DTVV01000109            1131 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1291372, whole genome shotgun sequence.
ACCESSION   DTVV01000109 DTVV01000000
VERSION     DTVV01000109.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1291372"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            209..>1131
                     /locus_tag="EYO86_01330"
     CDS             209..>1131
                     /locus_tag="EYO86_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIC41710.1"
                     /translation="MSDDKNRVYIFDTTMRDGEQSPGASMSLEEKLQISNLFDQIGID
                     IIEAGFPAASKGDFEAVSEVSKILKTSIPCGLSRHIKKDIDACNEALKIAERFRIHTF
                     ISTSPLHMKHKLNKSSEEVLEAIKEHVTYARKFTDDVEWSCEDGTRTDIDFMCRTVEL
                     AIKCGAKTINIPDTVGYSIPSEFTKIIKHLINNVPNIDKAILSTHCHNDLGLAVANSL
                     AGVVAGARQVECTINGIGERAGNAALEEIVMAMKTRNDLMPFHTNINTKLLSKASKLV
                     SSATYPVQFNKAIVGKNAFAHEAGIHQDGML"
BASE COUNT          430 a          160 c          216 g          325 t
ORIGIN      
        1 acacccaata gaaaaggttg gaaagaaact tagagatctt atgccttgga ttgggaagaa
       61 caaattagtt gataaggaaa aaaattagta aatttagtta aattttgttg aagaagcaaa
      121 aatcttttgc ataagacccg cttaggttgt aacataatca tttgtaaaaa ataaattttt
      181 ccaagttaaa acttggatta gagggataat gtccgacgac aaaaacagag tttatatatt
      241 cgatacaacc atgcgcgacg gggaacagtc tcctggcgcg tcgatgagtt tagaagaaaa
      301 acttcaaata tcaaatttat ttgatcaaat tggcattgac attatcgaag ctggttttcc
      361 tgctgcatca aaaggagatt ttgaagctgt tagcgaagtt agtaaaattt taaaaacatc
      421 aattccctgt ggtttatcta ggcatataaa aaaagatatt gatgcgtgta atgaggcatt
      481 aaaaatagct gaacgattta gaattcatac atttatctca actagcccac tacacatgaa
      541 acacaaattg aacaaaagtt ccgaagaagt tttagaagct ataaaggaac atgtaactta
      601 tgcaagaaaa tttactgatg atgtagagtg gtcttgtgaa gatggtacta gaactgatat
      661 tgattttatg tgcagaacag ttgagttagc aataaagtgc ggagctaaaa caattaatat
      721 accagatact gttggatatt caatcccttc tgaatttaca aaaattatta aacatttaat
      781 aaataatgtt ccaaatatag ataaagcaat tttatcaacc cattgtcata atgatttagg
      841 ccttgcagtt gcaaattctt tggctggagt tgttgcggga gcaagacagg tagaatgtac
      901 tataaatgga ataggagaga gagcaggtaa tgctgcactt gaagaaatag taatggcaat
      961 gaaaactaga aacgatttaa tgccgttcca taccaatatt aatacaaaat tattaagtaa
     1021 agcttctaag cttgtatcaa gcgctacata tccagttcaa tttaacaagg cgatagtagg
     1081 aaaaaacgct tttgcacatg aagccggaat ccaccaagat ggaatgttaa a
//
LOCUS       DTVV01000110            2784 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1296161, whole genome shotgun sequence.
ACCESSION   DTVV01000110 DTVV01000000
VERSION     DTVV01000110.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1296161"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="EYO86_01335"
     CDS             complement(<1..356)
                     /locus_tag="EYO86_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIC41711.1"
                     /translation="MADLNKIVKDLSSLSVIEAAELSKLLEEKWGVSAAAPVAVTAAP
                     AANVEESAAEEKTDFDVILTEVGSNKIAVIKETRTITGLGLKEAKDLVEGAPKPLKQG
                     VAKADAEDMKKSLEAAG"
     gene            complement(375..896)
                     /locus_tag="EYO86_01340"
     CDS             complement(375..896)
                     /locus_tag="EYO86_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIC41712.1"
                     /translation="MKRSDKNNFVQKLKDELNSSSSVIVAHYAGLSVLETDKLRKEMR
                     DNGAKFKVTKNRLTKLALADTPYESIADLFSGPTAIAYSSDPVLPAKVAVSFEKKLEN
                     FKIVGGSYDGEKIDKSKIYFLATLPSLDAIRGKLLGILMAPAKKIASVLQAPGGQLAR
                     LMSSRSEELGKLN"
     gene            complement(1086..1775)
                     /gene="rplA"
                     /locus_tag="EYO86_01345"
     CDS             complement(1086..1775)
                     /gene="rplA"
                     /locus_tag="EYO86_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIC41713.1"
                     /translation="MRQSKNRKNLIKKLEIGKVYEPIEAIKLLKENSYVKFDESLEVA
                     VNLKIDSNKTDQNVRGVLSLPKGTGKKIRVAVMAKGDKAKEASDAGADIVGDEDLAQQ
                     VTNGKINFDLLIATPDMMPTVGKIGKILGPKGLMPNPKLGTVTQDVRKAVSNAKAGQV
                     QFKNDKAGIIHAGIGKLNFTEEDLLQNLKSFYTSISKSKPDAVKGSFIKKVSIASTMG
                     FGLQINTTSLR"
     gene            complement(1776..2204)
                     /gene="rplK"
                     /locus_tag="EYO86_01350"
     CDS             complement(1776..2204)
                     /gene="rplK"
                     /locus_tag="EYO86_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIC41714.1"
                     /translation="MAKEILEFIKLQIPAGGANPSPPVGPALGQRGLNIMDFCKAFNE
                     KTKNLEKGAPIPVVITAYKDKTFNFVTKLPPVSHYLKKAAKIKKGNSTPGRTMEGKVT
                     MADVKKIAKEKMVDLNAIDLEGAANTVKGSARSMGMEVVE"
     gene            complement(2211..2744)
                     /gene="nusG"
                     /locus_tag="EYO86_01355"
     CDS             complement(2211..2744)
                     /gene="nusG"
                     /locus_tag="EYO86_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIC41715.1"
                     /translation="MSECRWYVLHAYSGYEKKVADSILDQAKKLGISEHVEDISVPTQ
                     NIVEVKRGVRINSERKIFPGYILIKMNLNDDTWHIIKNTPKLSGFLGAKGKPIPISNA
                     EAKRISEQVVDGVERSRPAVMYDIGEQVKVIDGPFASFNGEVERIDEEKARLRVAVSI
                     FGRLTPVDLEYSQVEKT"
BASE COUNT          787 a          570 c          392 g         1035 t
ORIGIN      
        1 ccagctgctt caagagattt tttcatatct tcagcatcag ctttggctac accttgtttt
       61 agaggttttg gtgctccttc aacaagatct tttgcttctt ttaatccaag acctgttatt
      121 gtacgagttt ctttaattac agcaatcttg ttagatccta cttcagttaa aataacatca
      181 aagtcagttt tctcttctgc agcagattct tcaacattag cagctggtgc agcagtaacg
      241 gcaacaggtg ctgcagctga cacaccccat ttttcttcta ataatttgct taattctgca
      301 gcttcaatca cagaaagaga cgataaatct tttactattt tatttaaatc agccattatt
      361 tttctccttt aaacctagtt taactttcct aattcttcac ttcgtgaact cattaatcgc
      421 gccaattgtc cgcctggtgc ctgtaataca gaagcaattt ttttcgctgg agccattagg
      481 ataccaagca attttcctct gatagcatca agtgatggaa gtgttgctaa aaaataaatt
      541 ttacttttat caattttttc gccatcgtaa ctacctccaa caattttaaa attttctaat
      601 tttttttcaa aactaacagc aactttagcc ggcaaaactg ggtcgcttga ataagcaata
      661 gcagttgggc cagaaaataa atcagcaatt gactcataag gagtatcagc tagagctagc
      721 ttagtaagtc tgttcttagt aactttgaat tttgctccgt tgtctctcat ttcttttcta
      781 agtttatctg tttcaagaac agataaacct gcgtaatgag ctacaataac tgaagaagaa
      841 ctgttgagtt catcttttag tttttgaacg aaattgtttt tgtcagaacg tttcactttc
      901 actccggttt gtgttgaggt taccctcagt tagctattgt taacaagaca gccaagttaa
      961 caacaacctg tcaagaagcc aatctaatga cttacttcag tctatgcagg aaattaagct
     1021 ttgcagcacc tacagtcttt gacaggtgac agcaacattt ctgctactat catattggat
     1081 taagattaac gcaaacttgt tgtatttatc tgtaatccaa atcccatagt tgaagctatg
     1141 gaaacttttt ttataaacga tccttttacc gcatcaggct tacttttaga aattgatgtg
     1201 taaaatgatt ttaaattttg taataaatcc tcttctgtaa aatttaattt tccaatacca
     1261 gcatgtatga ttccagcttt gtcattttta aactgaactt ggccagcttt agcatttgat
     1321 acagcttttc taacatcttg tgttactgta cctaatttag gatttggcat cagaccttta
     1381 ggtcctagta ttttgcctat tttaccaaca gttggcatca tgtcaggtgt agctataagc
     1441 aagtcaaaat taattttacc attagttact tgctgagcaa gatcttcgtc cccaacaata
     1501 tcagctcctg catcacttgc ctcctttgct ttgtctcctt ttgccatcac cgcaactcga
     1561 atttttttgc cagtaccttt tgggaggcta agtacaccac gtacattctg atcagtttta
     1621 tttgaatcaa tttttaaatt aacagcaact tcaagacttt catcaaactt gacatatgaa
     1681 ttttctttta ataatttaat agcttctatt ggttcatata cttttccaat ttcaagtttc
     1741 ttaattaaat tttttctatt ttttgattgt ctcatttact caacaacctc cattcccata
     1801 gaacgtgccg aacctttaac cgtatttgct gcaccttcta aatcaattgc atttaaatca
     1861 accatttttt cttttgcaat ttttttaaca tctgccattg taacttttcc ttccattgtt
     1921 cttccgggtg tagaattacc ttttttaatt tttgcagctt tttttaaata atgacttact
     1981 ggaggtaatt tagttacaaa attaaaagtt ttatctttgt aggctgtaat tacaacaggt
     2041 attggagccc ccttttcaag attttttgtt ttctcattaa acgctttaca aaaatccatg
     2101 atatttaatc ctcgttgtcc taaggcaggc ccaactggag gagatggatt agccccacct
     2161 gctggaattt gtaatttaat gaactctaaa atttctttag ccatagtaat ttaagtcttt
     2221 tctacctgag aatactctag atctactggt gttaatctgc caaatataga aactgcaact
     2281 cttaatctag ctttttcttc atcaattcgc tctacttctc cattaaagga tgcaaatggc
     2341 ccatctatta ccttaacctg ttcacctata tcatacatca cagctggtct tgatctctca
     2401 actccatcta caacctgctc tgatattctc ttagcttcag cattactaat cggtattggc
     2461 ttgcctttgg cacccaaaaa accacttaat ttaggagtgt tcttaattat atgccatgta
     2521 tcatcgttca gattcatttt aattaatata taaccgggaa atatttttct ctcagaattt
     2581 attcgaaccc ctcttttgac ctctacaata ttttgtgttg gaacagatat gtcttctaca
     2641 tgttcagaaa tgcctagttt ttttgcctga tccaaaatac tatcggctac ctttttttca
     2701 taacctgaat aagcatgtaa aacataccaa cgacattcac tcatttaaaa tcctgaacct
     2761 atttgaatta tttttttaat ggaa
//
LOCUS       DTVV01000111            1011 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1304597, whole genome shotgun sequence.
ACCESSION   DTVV01000111 DTVV01000000
VERSION     DTVV01000111.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1304597"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="EYO86_01360"
     CDS             <1..208
                     /locus_tag="EYO86_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIC41716.1"
                     /translation="NVLGVAGAAKQHYFLLVFGLVLSILLMATVANVISSWIEKYKWI
                     GWLGLLAILAVAIELIYSDVKIFL"
     gene            215..982
                     /locus_tag="EYO86_01365"
     CDS             215..982
                     /locus_tag="EYO86_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC41717.1"
                     /translation="MYLKKINLKNKTALVTGAGKGIGKACAVALAEVGANLIIISRTK
                     KDLDKVSKIIRKFNSKCVYYVCDVTNYHQIKEIINKQKRIDILVNNAGSNIPEHFTKV
                     QKKNMEQLVKLNTMATFNLAQLCTIKMLESKNRKKIGGSIINMSSQMGHVGGPMRSVY
                     NMTKFGLEGLTKGMAIDLAKNNIRVNSVCPTFVDTPMTKDFFKNKKFKVEVLKKIPLG
                     RIAKVSDVATAVAFLASDASSMITGTSLLIDGGWTAI"
BASE COUNT          385 a          127 c          191 g          308 t
ORIGIN      
        1 taatgtgctt ggagtagcag gggctgcaaa acaacattac ttcttgctag tctttggttt
       61 agttttatct atcctactta tggctacagt tgctaacgta atatccagtt ggattgaaaa
      121 atataaatgg ataggttggc taggattgtt ggcaatacta gcagttgcta tagaattaat
      181 ttatagtgac gttaaaatat ttttataaat aaatatgtac cttaaaaaaa ttaatttaaa
      241 gaataaaaca gctttggtca caggggccgg aaaaggtatt ggaaaagcat gcgcggtagc
      301 actagctgaa gtgggagcaa atctaattat aatcagtaga acaaaaaaag atttagataa
      361 agtttctaag ataataagaa aatttaattc aaaatgtgtt tactatgttt gcgacgtaac
      421 taattaccat cagattaaag aaataataaa taaacaaaaa agaattgata tattagttaa
      481 taatgctggt agcaatattc ctgaacattt tacaaaagtt caaaagaaaa acatggaaca
      541 acttgttaaa ctaaacacaa tggcaacatt taatttagct caattgtgta ccatcaaaat
      601 gctagagagc aaaaatagaa aaaaaattgg tggatcaata ataaatatgt catctcaaat
      661 gggtcatgtg ggtggcccaa tgagaagtgt ttacaacatg acaaaatttg gacttgaagg
      721 tttgacaaag ggaatggcta tagatttagc aaaaaataat attcgtgtta attcggtttg
      781 tccaactttt gttgatacac caatgactaa agattttttt aaaaataaaa aatttaaagt
      841 agaggtgctt aaaaaaattc cactcggtcg tattgccaaa gttagtgatg tggcaacggc
      901 tgttgctttt cttgcttcag acgcatcttc aatgattaca ggaacttcgc tcttgataga
      961 tggaggatgg actgcaatat aaaggagaaa taaacttatg agtaattttg a
//
LOCUS       DTVV01000112            1584 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1304827, whole genome shotgun sequence.
ACCESSION   DTVV01000112 DTVV01000000
VERSION     DTVV01000112.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1304827"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1..510
                     /gene="pgsA"
                     /locus_tag="EYO86_01370"
     CDS             1..510
                     /gene="pgsA"
                     /locus_tag="EYO86_01370"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIC41718.1"
                     /translation="MLVIPIIVALIYLKSPYYGWLAFILFCLASITDYFDGYIARIRN
                     EVSNLGTFLDPIADKLLVASVILILTSKEVITDWETIPALIILLREIAVSGLREYLAG
                     IKVSVPVSKISKLKTSLQLIALAILILSESGISFIPILFIGKIALWSAAILTLYTGYD
                     YLRSGLKHF"
     gene            510..758
                     /locus_tag="EYO86_01375"
     CDS             510..758
                     /locus_tag="EYO86_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIC41719.1"
                     /translation="MKIRYFAWLKNITNTDSEEFNEPSIRDVDSLKVFLCNKYPKLDP
                     YINKEKIVRIAINLEYTTTNDKLSFSDEVALFPPVSGG"
     gene            758..1183
                     /locus_tag="EYO86_01380"
     CDS             758..1183
                     /locus_tag="EYO86_01380"
                     /inference="COORDINATES: protein motif:HMM:PF02391.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIC41720.1"
                     /translation="MIKIQKEDFNLENEIAFIKSQHSNIGAVSTFVGYVRNINDKKNV
                     TSIDLEVYPEMAEKSLLEICDQAKKNWKLIDILVIHRFGALNVNQKIVLVATFAIHRH
                     DSMAACNYIMDYLKQDAPFWKKEYYDNDYKWLQNTTKTI"
     gene            complement(1167..>1584)
                     /locus_tag="EYO86_01385"
     CDS             complement(1167..>1584)
                     /locus_tag="EYO86_01385"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037028.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIC41721.1"
                     /translation="EAKASGPYIICTMSKEYAEKKGYHDALMLDYRGYVAEGTGANIF
                     FIKGNNIHTPIPDCFLNGITRQTVIELVRDKGLNLTERHILPDEINGYEEAFLTGTAA
                     EITPIKSIDAITYNTGNNTATYKLMNDFTNLVKSSL"
BASE COUNT          527 a          257 c          269 g          531 t
ORIGIN      
        1 atattggtaa tcccaattat cgttgcgttg atatatttaa agagcccata ttatggttgg
       61 ttggctttta tcttattctg tcttgccagc ataacagatt actttgatgg gtatatagcg
      121 agaatacgga atgaagtttc caatcttgga acattccttg atcccattgc cgataaatta
      181 ttagttgcct cggtaatttt gattttaact tcaaaagagg taatcactga ttgggaaact
      241 attcccgcac ttattatttt attacgggaa atcgccgttt caggcttaag agaatacctt
      301 gcgggcatta aagtaagcgt gcctgtatcc aagatctcaa agttaaaaac atcgctgcaa
      361 ttaattgcct tagcaatatt aattctcagt gaaagtggga tttcttttat tccaattttg
      421 tttattggta aaattgcttt gtggagtgct gccatattga ccttatatac agggtatgat
      481 tatttaagat caggattgaa acatttttaa tgaaaattag atattttgcc tggttaaaaa
      541 atattaccaa tactgattca gaagagttta atgaaccatc aatccgtgat gttgattcct
      601 taaaagtatt tctctgcaat aagtatccca aactcgatcc atatatcaat aaggaaaaaa
      661 ttgtccgcat cgcaattaac ttagaatata ctaccacgaa cgacaaactt tcatttagtg
      721 acgaagttgc tttatttcca ccggtcagtg gcggttaatg attaaaattc aaaaagaaga
      781 ttttaatttg gaaaatgaaa ttgcttttat taaatcacag catagcaata ttggggctgt
      841 atccactttt gtaggatatg taagaaatat taacgataaa aaaaatgtta catcaattga
      901 cttggaagta tatccagaaa tggcagaaaa atcactcttg gaaatctgtg atcaagcaaa
      961 aaaaaattgg aaacttatag atattttagt tattcatcgg tttggggcac ttaatgtaaa
     1021 tcaaaaaata gttttagtag cgacatttgc cattcatcga catgacagca tggctgcctg
     1081 taattatatc atggattatt taaaacaaga tgcaccattt tggaaaaaag aatattatga
     1141 caatgattac aaatggctac aaaatactac aaagacgatt taactaaatt tgtaaaatca
     1201 ttcatcagtt tataagtagc ggtattattg ccagtgttat aggttattgc atcgatagac
     1261 ttaattgggg taatttccgc tgcggtaccc gttaaaaaag cttcttcata tccattaatt
     1321 tcatcaggaa gaatatgtcg ctcggttaaa ttcaaacctt tatcccttac caattcaatg
     1381 acagtttgac gcgttattcc atttagaaaa caatcaggaa ttggggtgtg aatattgtta
     1441 cctttaataa aaaaaatatt tgcacccgtt ccttcggcta cataacctcg gtaatccaac
     1501 attagagcat cgtgataccc ttttttttca gcatactcct tggacattgt acatattata
     1561 tatggaccag atgctttagc ttca
//
LOCUS       DTVV01000113            1367 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1305376, whole genome shotgun sequence.
ACCESSION   DTVV01000113 DTVV01000000
VERSION     DTVV01000113.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1305376"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /gene="rplB"
                     /locus_tag="EYO86_01390"
     CDS             complement(<1..138)
                     /gene="rplB"
                     /locus_tag="EYO86_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIC41722.1"
                     /translation="MTLKILKPTTPGQRGTVLVSKSHLWKGEPYKKLVQPLHSTGGRN
                     NQ"
     gene            complement(139..456)
                     /locus_tag="EYO86_01395"
     CDS             complement(139..456)
                     /locus_tag="EYO86_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIC41723.1"
                     /translation="MNNKLKSTTEERALNIIYRPLMTEKSTNLNQYNQYSFIVSKDSN
                     VIEIKSAIERIFKVKVTKVNTSILKGKIKSFKGSSGYRKDLKKAIVTLAEGNTIDSSL
                     DIK"
     gene            complement(453..1076)
                     /gene="rplD"
                     /locus_tag="EYO86_01400"
     CDS             complement(453..1076)
                     /gene="rplD"
                     /locus_tag="EYO86_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIC41724.1"
                     /translation="MKKQVVNLLNKNVSEIELSDSIFGIKVFPDLIHQYIRYQNAKLR
                     QGSHKTKTRSEVKGRAKKPFSQKGTGNARQGSSKPPHFRGGATSMGPQNRDHSFSLNK
                     KEKTLALKCALSNKADKEEIIFIDSLKVDNHKTKNLHKSLKNFDFNSALFIHIDDASN
                     ENFKRASSNIPRLAMLSDKGLNVRDLMTFEKIFIETKAVEKIIKRLS"
     gene            complement(1076..>1367)
                     /locus_tag="EYO86_01405"
     CDS             complement(1076..>1367)
                     /locus_tag="EYO86_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIC41725.1"
                     /translation="KGFAGVMKRHNFSGQRASHGVSLSHRKAGSTGNSQDPGRVFKGK
                     KMEGHMGNVRVTKQNLKIVDIDLANNLIAIKGSVPGKKNTTVFIKDSVKGKK"
BASE COUNT          386 a          232 c          195 g          554 t
ORIGIN      
        1 ctgattattt ctacctcctg tagaatgcaa aggttgaaca agttttttgt aaggttcacc
       61 tttccataaa tgagactttg acaccaaaac tgttcctctt tgcccaggtg ttgttggttt
      121 taaaattttt aatgtcattt atttaatatc caaggatgaa tctattgtgt tgccttcagc
      181 aagtgtaaca atagcttttt ttaaatcttt cctatacccc gaacttcctt tgaaagattt
      241 aattttgcct ttcaaaattg aagtattcac ttttgtaact ttgactttaa atattctttc
      301 aattgcactt tttatctcaa taacattaga atctttgctg acaataaaag aatattgatt
      361 gtattgattc aaatttgttg atttttcagt cataagaggt ctgtatataa tgtttaatgc
      421 acgttcttca gtagttgatt ttaatttgtt attcatgaaa gcctcttgat aattttttca
      481 acagcttttg tttcaataaa tattttttca aatgtcatta agtctcttac attcaaacct
      541 ttatcagata gcatcgcaag tcttggtata ttggatgaag cacgtttaaa attttcatta
      601 ctagcatcat caatatggat aaataaagca gaattaaaat caaagttttt taaacttttg
      661 tgtaaatttt ttgttttgtg attatctact ttcaaagagt caataaaaat aatttcctcc
      721 ttgtctgctt tatttgataa agcgcatttc aaggcaagag ttttttcctt tttatttaaa
      781 gaaaaagaat ggtctctatt ttgaggtccc attgaagttg cccctcctct aaaatgagga
      841 ggttttgaac taccctgcct tgcatttcct gtgcctttct gtgaaaaagg ttttttggct
      901 ctccctttca cttctgatct tgtcttggtt ttatggcttc cttggcgtaa ttttgcattt
      961 tggtatcgaa tatattgatg aattaaatct ggaaaaactt tgattccaaa aattgaatca
     1021 gatagctcaa tttctgagac atttttattg aggagattca caacctgttt tttcattatt
     1081 tttttccctt aactgaatct ttaatgaaaa cagtggtatt ttttttacca ggtactgacc
     1141 ctttaattgc tattaaatta ttagctagat cgatatcaac aatttttaga ttttgcttag
     1201 ttactctcac attgcccatg tgtccctcca ttttttttcc tttaaaaact ctgccaggat
     1261 cttgactatt acccgttgat ccagcttttc tgtgtgaaag agaaacacca tgggatgctc
     1321 tttgaccact aaaattatga cgcttcatta ctcctgcaaa accttta
//
LOCUS       DTVV01000114            1140 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1305930, whole genome shotgun sequence.
ACCESSION   DTVV01000114 DTVV01000000
VERSION     DTVV01000114.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1305930"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="EYO86_01410"
     CDS             complement(<1..566)
                     /locus_tag="EYO86_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013695429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC41726.1"
                     /translation="MDLLIFKAPILIVQASMDGILLGILFALIAYGMALQWGVMNIIN
                     IAQGELVIMGGYIAYFMYVAGIHPAFGVIVSPIIMYFVGVGLYKLVINKVVDRDLFIS
                     ILATFGISILAQQLMNFIFGADVVVAQSNFGTTMLFDNSVTLPNSKIFSAAVSIVFAI
                     GLVFYMKKSKLGRAIRATAQNARAAKILG"
     gene            complement(621..>1140)
                     /locus_tag="EYO86_01415"
     CDS             complement(621..>1140)
                     /locus_tag="EYO86_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC41727.1"
                     /translation="SKGALTAIRQIAEMKVDVPMLAMTHCDASKLAKQHGKNSEYALC
                     AAQWHKELSYKDDYFGNGLKFDKDFNALYGYAPPYQAAESAAALLVYKDAFERANSTD
                     KEKVRDALAKTDMETFYGNVKFGDGGQNIAKPMVLFQVRCEGDTCANKLVAPTKWASH
                     KLVHPIPKWSER"
BASE COUNT          378 a          227 c          162 g          373 t
ORIGIN      
        1 cctaaaatct ttgcagctct tgcattttgc gctgtagctc taattgcacg acctagtttt
       61 gattttttca tgtaaaaaac taaaccaatt gcaaatacaa tacttacagc tgctgaaaat
      121 atttttgaat ttggaagtgt taccgaatta tcaaataaca ttgtggttcc aaaattagat
      181 tgagcgacaa caacgtctgc accaaatata aaattcatta attgctgagc taaaatactt
      241 attccaaacg ttgctaaaat tgaaataaac aaatctctat caacaacttt attaattact
      301 aatttgtaaa gtcctactcc aacaaaatac ataattattg gagaaacaat tacgccaaat
      361 gcaggatgga ttccggccac atacatgaaa taggcaatat aaccacccat aataactaat
      421 tcaccttgtg caatatttat tatattcatc acgccccact gcaatgccat accgtatgca
      481 ataagtgcga aaaggattcc aagcaaaata ccatccatgg atgcttgaac tattaatatt
      541 ggtgccttaa aaattaataa atccatcgag ttgaaaaaaa atgcccccta gaaattaggg
      601 ggcattaaat attatttttt ttatctttca gaccacttag gaattggatg cactaattta
      661 tgcgatgccc attttgttgg agcaactaat ttattagcac aagtatctcc ttcgcatctt
      721 acttggaata gtaccattgg cttagctata ttttgaccgc catcaccaaa tttcacattt
      781 ccataaaatg tttccatatc agtttttgct aaagcatctc taactttttc tttatctgta
      841 gagtttgctc tttcaaatgc atctttataa accaaaagtg ctgctgcaga ttctgccgcc
      901 tgataaggtg gtgcatatcc atacaaagca ttaaaatctt tatcaaattt taatccattg
      961 ccaaaataat catctttgta agacaattcc ttgtgccatt gagctgcaca aagagcatac
     1021 tcagagtttt taccatgttg cttagcaagt tttgatgcat cgcaatgtgt cattgctaac
     1081 ataggaacat ctactttcat ttcagcaatt tgtctgatcg cagttaatgc ccctttagag
//
LOCUS       DTVV01000115            1890 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1309835, whole genome shotgun sequence.
ACCESSION   DTVV01000115 DTVV01000000
VERSION     DTVV01000115.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1309835"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            360..>1890
                     /locus_tag="EYO86_01420"
     CDS             360..>1890
                     /locus_tag="EYO86_01420"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01619.16,HMM:PF14850.4"
                     /note="proline utilization protein A; multifunctional
                     protein that functions in proline catabolism in the first
                     two enzymatic steps resulting in the conversion of proline
                     to glutamate; in Escherichia coli this protein self
                     regulates transcription via a DNA-binding domain at the
                     N-terminus but the protein from Pseudomonas does not have
                     this domain; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HIC41728.1"
                     /translation="MNEMNPEITFDEINKIAYASESLVSKNLLNYLKPEYNKRLVNIE
                     DRANEIVKAVRKSIKSDSPVENLLQEYELNTKEGTVLLCLAEALLRIPDKKTMDRLLE
                     DKFSSVDWKKHISADKGIFVNASSWAFFLTGNILDKKITDKTKLEETYKSLLKKSTEP
                     IIRTAVKKAVTILARQFVFKPTIEEGMKFTESEKYKKNIFSFDMLGEGARTMEDAEKY
                     FQDYIKSIHAVGKLLNNSSEIKYANGVSIKISALHPRYERNKSEDLEKELLPKLVKLC
                     EISKQYNIQLCIDAEENYRLILSLKILDKLSSNQNLLGWNGLGLAVQAYQKRAFYVID
                     WLDKLARRDNRVINVRLVKGAYWDSEIKLAQELGVPNYPVFTRKSLTDLSWMACAKKL
                     FSLQKNIFPQFATHNAYSIAFIEEFGKDKIFEFQRIHGMADKIHEYFNRYSSDNYQKC
                     RIYAPVGEYNDLLPYLMRRLLENGANTSFVNKINDPKLDIKDIIADPLDIIDQYSQIP
                     NP"
BASE COUNT          714 a          250 c          325 g          601 t
ORIGIN      
        1 catttgggtt gatggtaaga aatgcgctct gggatcagtt ggacaatgtg actaaactat
       61 tcacaaaaaa agatataaga tctttaataa aatggaagaa ttgatcctaa gccaactcta
      121 gataagttgt cgtagattaa tatagttgaa atttatgatt tagttaatta gacaataagt
      181 aatgaaaaaa atattagttt ttatttttat tttaaccttt ttaatttctt gtcagcttag
      241 cacttctaaa acaaccatta ccacagataa ttttgaaaaa gattcaaaag caaatgttac
      301 atttagcgga ggagtaaata acggaatctg tataaattgc agtcaataga ttgaaaaata
      361 tgaacgaaat gaatccagaa attacttttg atgagattaa taaaattgct tatgcttcag
      421 aatctctagt ttcaaaaaat ttattaaatt atttaaaacc agaatacaat aaacgccttg
      481 ttaacattga agatagggca aatgaaattg ttaaagctgt ccgtaaatca ataaagtcag
      541 atagtcctgt agaaaattta cttcaggagt atgagttgaa tactaaagaa ggcactgttt
      601 tgctttgctt ggcggaggcc ctgctgagaa ttcctgataa aaaaacgatg gatcgattat
      661 tggaggataa attttcatct gttgattgga aaaaacatat tagtgcggat aaaggaattt
      721 ttgtcaatgc gtcctcgtgg gctttttttc ttactggaaa catacttgat aaaaaaataa
      781 ctgacaaaac gaaacttgaa gaaacatata aaagtttatt aaaaaaaagt accgagccta
      841 taattcgtac agcagttaaa aaagcagtta ctatcttagc aagacagttt gtttttaaac
      901 caaccatcga ggaaggaatg aaatttactg aatcagagaa atataaaaaa aatatttttt
      961 cttttgacat gcttggcgaa ggcgcaagaa ccatggaaga tgctgaaaag tattttcaag
     1021 attatattaa atccatacat gcagtgggca agctgctaaa taattcttct gaaattaaat
     1081 atgcgaatgg tgtttcaata aagatttccg cattacatcc ccggtatgaa agaaataaat
     1141 ctgaagattt agaaaaagaa cttttaccta aattagtgaa attatgtgaa atatcaaagc
     1201 aatataatat tcaattgtgc attgatgcag aagaaaatta tagacttatt ttgtctttaa
     1261 aaattttaga taaactatct tctaatcaga atttattagg atggaacggt cttggattag
     1321 cggttcaagc ttatcaaaaa agagcttttt atgtgattga ttggttagat aagttggcaa
     1381 gaagagacaa cagagtaata aatgttcggt tagtaaaagg ggcttactgg gatagtgaga
     1441 ttaagttagc tcaagaatta ggcgtaccaa attatccagt atttacaaga aaatctttga
     1501 ctgatttatc ctggatggca tgcgctaaaa aattattttc cttgcaaaaa aatatttttc
     1561 ctcagtttgc cacacataac gcttattcaa tagcttttat tgaagagttt ggaaaagaca
     1621 aaatttttga attccaaaga attcatggaa tggcagataa aattcatgaa tactttaatc
     1681 gttattctag tgataattac caaaaatgca gaatatatgc tcctgttgga gagtataatg
     1741 atttgcttcc atatttaatg cgtagattat tagagaatgg cgccaacact tcttttgtaa
     1801 ataaaattaa tgatccaaaa ctagacatta aggatataat tgcagaccct ttggatatca
     1861 ttgatcaata ttcccaaatt cctaacccac
//
LOCUS       DTVV01000116            1160 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1313796, whole genome shotgun sequence.
ACCESSION   DTVV01000116 DTVV01000000
VERSION     DTVV01000116.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1313796"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(22..465)
                     /locus_tag="EYO86_01425"
     CDS             complement(22..465)
                     /locus_tag="EYO86_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HIC41729.1"
                     /translation="MNLNVYLSGEIHTDWRSRLKEGCEAHNLSIVFTSAVTDHEASDA
                     AGDVLGVESINYWRDHKSAKVNAIRIKTLIENCDVAVVRFGDKYKQWNAAFDAGYCSA
                     LGKPYITLHDEDIVHPLKEVDGSAMAWATTPGQVIEILKYVLTEE"
     gene            787..>1160
                     /locus_tag="EYO86_01430"
     CDS             787..>1160
                     /locus_tag="EYO86_01430"
                     /inference="COORDINATES: protein motif:HMM:PF01863.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HIC41730.1"
                     /translation="MNYHLIRSKRKTLSLKINSNAELIVRAPNRLSVKKIEQFINEKS
                     NWIDKKSTSIHAKKLQKPHYTKGEKFLYLGGEYPLSVNTSHAKGLSFDGQMFSLDVGE
                     KKEFLAWYKAAFKKVALPRLDY"
BASE COUNT          405 a          212 c          179 g          364 t
ORIGIN      
        1 ccctaaagtt ttcagccagg cttattcttc agtcagaaca tattttagaa tttcaataac
       61 ttgacctggt gttgtggccc atgccattgc cgaaccatca acttccttca gtggatgcac
      121 tatgtcttca tcatggaggg tgatgtaagg cttgcctaat gcagagcaat acccggcatc
      181 aaaagctgca ttccactgct tgtacttatc tccaaaacgc accacggcta cgtcacagtt
      241 ttcaataagg gtcttaattc gtattgcgtt aacttttgca gatttatgat cgcgccagta
      301 attaatgctt tcaacaccaa gcacatcacc tgcagcatca cttgcttcat gatctgtgac
      361 ggcagacgta aaaacaatag ataaattatg cgcttcacaa ccttctttta ggcgactcct
      421 ccaatctgta tgtatttcac cagatagata tacgttcaaa ttcatattaa atcctatatt
      481 tatttttgat aaaaaaatag aagtttactg caaaatgagg ttgacattca aacctttaat
      541 aaacttaact aaagtatcaa ataaagataa tatttacacc ttattgaatt tttaaaattt
      601 aggaaataat taaaattaga taaatatatt ctaaataaag gccttttctg cactaaaaat
      661 agctataaat cctcaaaata acctatattt caatattcag gtgtaactta cagttagcat
      721 aataaaaatt taggaaattt taacaatggg ccaattttta ggtttttatc gggttaaata
      781 gcgattatga attatcatct aattcgctct aaacgaaaaa cattgagttt gaaaattaac
      841 agcaatgctg aactaattgt tcgtgcgcca aatcgattat ctgttaaaaa aatagagcaa
      901 tttattaatg aaaaatccaa ctggattgat aaaaaatcaa catctatcca cgctaaaaaa
      961 cttcaaaaac ctcattatac aaaaggtgaa aaattcttat atttaggtgg tgaatacccc
     1021 ttaagtgtta acacctccca tgcgaaaggc ttgagctttg atgggcaaat gttcagcctt
     1081 gacgtgggtg aaaaaaaaga atttttagct tggtataaag ctgcttttaa aaaggtagcc
     1141 ctaccgaggt tagattacta
//
LOCUS       DTVV01000117            1199 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1315723, whole genome shotgun sequence.
ACCESSION   DTVV01000117 DTVV01000000
VERSION     DTVV01000117.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1315723"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /gene="trpD"
                     /locus_tag="EYO86_01435"
     CDS             <1..933
                     /gene="trpD"
                     /locus_tag="EYO86_01435"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIC41731.1"
                     /translation="DLIMNGEISEVEAAGILIGLKTKSESKDEILGAAKSMREKSLKI
                     SSPENTIDTCGTGGDMSGTLNISTSAAIVAASAGAKVAKHGNRSISSRSGSADMLEKI
                     GYKISADQSYLENSLSKNNFCFLFAQYHHSAMKHVINVRKQLGTRTIFNLLGPLTNPA
                     NTKKQLLGVFDSKWVKIHCEVLKDLGSNHALVVHGEDGLDEISLSAHTKIAELKDGLI
                     TEYVFNPQDYGYTLIKNSDIQGKDPDYNAKTFMQMLEGLNKKFQHIVELNAGAALYLS
                     QQSKSFKEGFKLARHVIETNITKQFVKKIIKTNG"
     gene            926..>1199
                     /locus_tag="EYO86_01440"
     CDS             926..>1199
                     /locus_tag="EYO86_01440"
                     /inference="COORDINATES: protein motif:HMM:PF00218.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase 2"
                     /protein_id="HIC41732.1"
                     /translation="MDELLKDICQKKKYELERTKSKCSFQTLEKLLPKKNNRGLKKIL
                     NDSQKNKNINIIAEIKKASPSAGEIIKEYVPEDIATQYEKSGAGAIS"
BASE COUNT          474 a          163 c          211 g          351 t
ORIGIN      
        1 gatctaatta tgaatggtga aatttcagaa gtagaggcag cgggtatctt aatagggtta
       61 aaaacaaagt cagaaagcaa agatgaaata ctgggcgcag caaaatcaat gcgtgaaaaa
      121 tcattaaaga tatcttctcc tgaaaatact atagatacct gtggaacagg tggtgatatg
      181 agtggcactt tgaatatttc cactagtgcc gcaattgtgg cagcaagtgc tggtgctaaa
      241 gtcgcaaaac atggtaaccg ttcaattagt tccagatctg gatctgcaga tatgcttgaa
      301 aaaattggat ataaaatctc cgcagatcaa agttatcttg aaaactcatt gtcaaaaaat
      361 aatttttgtt ttttatttgc tcaataccat cattctgcaa tgaagcatgt tataaatgtt
      421 cgcaaacaat taggtacacg aacaattttt aatttacttg gaccattgac taacccagct
      481 aatacgaaaa aacaattgct tggagtattt gatagcaaat gggtaaaaat tcattgtgaa
      541 gttttaaaag atttaggatc caatcatgct ctagttgttc acggagagga tggtttagat
      601 gaaatttctt taagtgccca tactaaaata gcagaattaa aggatggcct gatcacagaa
      661 tatgttttta atcctcaaga ttatggttac acattaataa agaattcgga tattcaaggt
      721 aaagatcctg attataacgc aaaaactttt atgcaaatgt tggaaggttt gaataaaaaa
      781 tttcaacata ttgttgaatt aaatgctggt gcggctttat atttatctca acaatcaaaa
      841 tcttttaaag aaggttttaa attggccaga catgtaattg aaacaaatat aacaaaacag
      901 tttgtaaaaa aaataattaa aactaatgga tgaattatta aaagatatct gtcaaaaaaa
      961 gaagtatgaa ctagaaagga ctaaatcaaa gtgttcattt caaacccttg aaaaattatt
     1021 acctaaaaaa aataatcgag gattaaaaaa aatacttaat gattcgcaaa aaaataaaaa
     1081 tataaatatt attgctgaaa ttaaaaaagc aagtcctagt gccggtgaaa taataaagga
     1141 atatgttcct gaagatattg ccacccaata tgagaaatca ggagctgggg ctatatcaa
//
LOCUS       DTVV01000118            1073 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1316104, whole genome shotgun sequence.
ACCESSION   DTVV01000118 DTVV01000000
VERSION     DTVV01000118.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1316104"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="EYO86_01445"
     CDS             complement(<1..624)
                     /locus_tag="EYO86_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41733.1"
                     /translation="MSAEKFDPFVSEWVSFSKNSKHNLIEKSLKLAQILEYPDLNISK
                     YIEKINEIGNSLKLKIKYVKNSTYLISMLNEHVFEKYGFQGDDEDYYDPRNNFLNAVI
                     DKKTGIPITLSIIYSEVAKYIGLDLKIVGFPGHVVVKYEEEMIIDPFYSGRLLTINDL
                     EEILYRNFGDGVEFIPEYLNTATTDQILTRLLRNLKNAYTQSYAYVNA"
     gene            complement(636..>1073)
                     /locus_tag="EYO86_01450"
     CDS             complement(636..>1073)
                     /locus_tag="EYO86_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41734.1"
                     /translation="LAKRANLFFMLNPDNFITNVMGPDVMTYTKVEIDPKITEFLPIL
                     QEIYQRWLKPIQSQHAIFTTMEGMAEFVVQQILKDDTNFQNYLTTFAGTDYSAYSVKK
                     SIGKEFTEFIFGKFGKSTFEKLIMNPPNTKELKNPQIYLNRIK"
BASE COUNT          344 a          166 c          150 g          413 t
ORIGIN      
        1 agcattaaca tatgcatatg attgtgtgta cgcatttttt agatttctta ataatctagt
       61 caaaatttga tccgtagttg cagtgtttag atattcagga ataaattcta caccatcacc
      121 aaagtttcta tacaaaattt cttccaagtc attaatcgta agtaatcttc cactgtaaaa
      181 tggatcgata atcatttctt cttcgtattt tacaactaca tgtcccggaa atcccacgat
      241 cttaagatca agaccgatat actttgcaac ttcagagtat atgatggaaa gagtgatagg
      301 aataccagtc tttttgtcaa taactgcatt taaaaaatta tttcttggat cgtaataatc
      361 ttcatcatca ccttgaaacc catatttttc aaaaacatgt tcattaagca ttgaaataag
      421 atacgtagag tttttaacat attttatttt caactttaac gaattaccaa tttcatttat
      481 cttttcgatg tattttgata tatttagatc agggtattcc aagatttgtg ccagtttaag
      541 ggatttttca attaaattat gctttgaatt tttcgaaaaa gaaacccatt ctgaaacaaa
      601 tggatcaaat ttttctgctg acaataaatt aaatattatt ttattctatt tagatatatt
      661 tgtggatttt ttaattcttt tgtgttaggt gggttcatta tgagtttttc gaacgtgctt
      721 ttaccaaatt taccaaatat gaattcagta aattctttac ctatgctttt ttttacacta
      781 tatgcagagt aatcagtacc tgcaaacgta gtaagataat tttgaaaatt tgtatcatcc
      841 ttcaatattt gttgtactac aaattctgcc atgccttcca tagtagtaaa tattgcatgt
      901 tgagactgta taggttttaa ccatctctgg taaatctctt gcaagattgg taaaaattcc
      961 gtaatctttg gatcaatttc aactttggtg tatgtcatta catccggtcc cataacattg
     1021 gtgataaaat tatctgggtt tagcataaaa aacaaattag ctctttttgc aag
//
LOCUS       DTVV01000119            1397 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1316925, whole genome shotgun sequence.
ACCESSION   DTVV01000119 DTVV01000000
VERSION     DTVV01000119.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1316925"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="EYO86_01455"
     CDS             complement(<1..523)
                     /locus_tag="EYO86_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIC41735.1"
                     /translation="MESVQNYILKCKNNVINGNGVTKDEASILFNLDNQFMDKLINAA
                     NSITRNFHGSKVDIEELANIKKNFCSEDCAYCAQSSFFNTKINDYQLPPPDQIVKQAI
                     NAKNDGADSFCLVAAWREPNNKDFENVCKIIHEINKKVGISIECSLGFLTFHQAKKLK
                     ELGVKRYNHNLETA"
     gene            654..>1397
                     /locus_tag="EYO86_01460"
     CDS             654..>1397
                     /locus_tag="EYO86_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC41736.1"
                     /translation="MLEWSKFPKIVSGNGMWLIDEDGNKFLDAVASMWCNVWGHSKKE
                     LINVIVSQTKKLQHSPLFNFTNEPIEILAKKLTKLTPGMSKVFFSDNGSTAMEISIKI
                     ALQYWNNLGQKNKIKIASLENGYHGDTFGAMSVGYLPEFFSKFNSKLFSTTRFSVPNS
                     YHLPANYTIDEFTSYCLEKIEKKLREDEAIAAFVMESGAQVAGGVNIYPDKFQKQIDV
                     ICKKYDVLFVLDEIATGFGRLGSMIEYKSQ"
BASE COUNT          475 a          192 c          220 g          510 t
ORIGIN      
        1 gagctgtttc taaattatga ttatatcttt ttacacctaa ttcttttagt tttttggctt
       61 gatgaaaagt tagaaatccg agactacatt caatcgaaat tccaactttc ttattaatct
      121 catgaatgat tttgcataca ttctcaaaat ctttgttatt tggttcacgc cacgcggcaa
      181 ctaagcaaaa tgaatctgca ccatcatttt ttgcattaat ggcctgctta acaatttgat
      241 caggaggtgg gagttggtaa tcattaattt ttgtattaaa aaatgaagat tgtgcacaat
      301 acgcacaatc ctcactacaa aaattttttt taatattagc caattcttca atatccactt
      361 ttgaaccatg aaaattcctt gttatggaat tagcagcatt tatcaattta tccataaatt
      421 ggttatctaa attaaacagt attgatgcct catcttttgt aacaccatta ccatttatta
      481 cattattttt acattttaaa atataatttt gaacactttc catatttttt ttgtaatatg
      541 tctataatta agcgttaacc agcatatttt ttgaagttaa ctattaatta atagtaattt
      601 gtgctaatgc aatagtgaaa agcgagagtt ttgtttggca tccaaataca caaatgttgg
      661 agtggtcaaa atttcctaag attgttagcg gtaatggtat gtggttaatt gatgaagatg
      721 ggaataaatt tcttgatgct gttgcaagta tgtggtgcaa tgtttggggt cattctaaaa
      781 aggaattaat taatgtaata gtcagtcaga caaaaaaatt acagcattct cctttgttta
      841 attttacaaa cgaacctatt gaaattttag caaaaaaatt aacaaaactt actccaggta
      901 tgtcaaaggt attcttttca gataatggtt caactgctat ggaaatttct ataaaaattg
      961 cattacaata ttggaataat ctaggtcaaa aaaataagat aaaaatcgct tcacttgaaa
     1021 atggttatca tggtgacaca ttcggtgcta tgtctgtagg ttatttacct gaattttttt
     1081 caaagtttaa ttctaaatta ttctcaacaa ctagattcag tgttcctaat tcgtatcatt
     1141 tacctgcaaa ttatacaatt gatgaattca caagttattg tttagagaaa attgaaaaaa
     1201 aattacgtga agatgaggca attgctgcat ttgttatgga aagtggtgca caggtagcag
     1261 gtggtgtaaa tatctatcca gataaatttc aaaaacaaat tgatgtgatt tgtaaaaaat
     1321 atgatgtttt atttgtcttg gatgaaattg ctaccggatt tggtagactt ggttcaatga
     1381 ttgaatacaa aagccaa
//
LOCUS       DTVV01000120            1442 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1318463, whole genome shotgun sequence.
ACCESSION   DTVV01000120 DTVV01000000
VERSION     DTVV01000120.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1318463"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="EYO86_01465"
     CDS             complement(<1..101)
                     /locus_tag="EYO86_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIC41737.1"
                     /translation="MSIYLPIAEMNVNILLIVLLGMLVGGLSGLFGVG"
     gene            complement(155..778)
                     /gene="mobA"
                     /locus_tag="EYO86_01470"
     CDS             complement(155..778)
                     /gene="mobA"
                     /locus_tag="EYO86_01470"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIC41738.1"
                     /translation="MDNLNKEISAVIMIGGQSKRIGGGIKSLITFNNKSIFDRILERL
                     VSQVDKIIINCNDQERSFEKYRLPILKDLKQGYLGPLAGIHSAMHWLSLNDRQTKWLI
                     TIPGDTPFIPMNIVAQFKSKMSKQTKIILAQSGQKTHPIIGAWHISLFSSLDEHLNKG
                     TRKILTWAHNHPIEYINFDTKPYDPFFNINFKEDITEATQIEDFHTS"
     gene            complement(778..>1442)
                     /gene="fdhD"
                     /locus_tag="EYO86_01475"
     CDS             complement(778..>1442)
                     /gene="fdhD"
                     /locus_tag="EYO86_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIC41739.1"
                     /translation="VTLMSIPDYPRYLAVGYLLNQNMLKQNDIIKSIDYDKDINVIVV
                     RTKRETNYEKNLKKKITTSGCAQGTIFGDIYDEIQKIKITSKVTIKHDWIYEISKKIN
                     LTPSLYLKAGAVHGCVIMNKNHPIIYMEDVGRHNAVDKIAGYMFLNNINPMNKIFYTT
                     GRLTSEMIIKTVKMGMPILISRSGFTSWGVELAQKTNLTLIGRAKGKKYLVLSGHHRI
                     KR"
BASE COUNT          443 a          232 c          214 g          553 t
ORIGIN      
        1 ccgacaccaa acaaacctga gagccctcca accaacatgc ctaaaagtac tataagcagt
       61 atgttgacat tcatttcagc aattgggagg taaattgaca ctccaaatac tatattctta
      121 taaatttaat aattctatca ttttaagtat ttttttaaga agtatgaaaa tcttctattt
      181 gcgtagcctc agtgatgtct tctttaaagt tgatattaaa aaaagggtca tagggttttg
      241 tatcaaaatt tatatattca attgggtgat tatgagccca tgttaaaatt tttcttgttc
      301 ctttgtttag atgttcatca aggctcgaga acaaactaat atgccatgca cctattatag
      361 ggtgagtttt ctgtcctgat tgggcaagaa taatttttgt ttgctttgac attttacttt
      421 tgaattgggc aacaatattc ataggaatga agggagtgtc accaggtata gttataagcc
      481 attttgtttg gcgatcatta aggctaagcc aatgcatagc ggaatggatg ccagctaaag
      541 ggccaaggta gccttgtttt aaatctttta aaataggtaa tcgatatttt tcaaaacttc
      601 tttcttggtc attgcagttt ataattattt tatctacttg agaaactaat ctctcaagaa
      661 ttcgatcaaa tatactttta ttattaaaag taattaaaga tttaatgcct ccccctattc
      721 tttttgattg accaccaatc ataataacag ctgatatttc cttatttaaa ttatccatta
      781 tcgtttaatt ctatgatgac ctgataaaac aagatatttt tttcctttag ctctacctat
      841 caaggtaaga tttgtttttt gggcaagttc aacaccccat gaagtgaatc cagagcgaga
      901 tattaaaatg ggcattccca ttttgactgt tttaataatc atttcacttg taagtcttcc
      961 tgtcgtgtaa aaaattttat tcattggatt gatattattt aaaaacatgt atcctgctat
     1021 tttatcaacc gcattatgcc ttccaacatc ttccatataa attattggat gatttttatt
     1081 cattataaca catccgtgta ctgcacctgc cttcaagtac aaactcggtg taagattaat
     1141 ttttttagaa atttcatata tccaatcatg cttaatggtt acttttgatg taatttttat
     1201 tttttgtatc tcgtcataaa tatcaccaaa aattgttcct tgagcgcaac cgcttgtagt
     1261 aatttttttc tttaaatttt tttcataatt tgtttctctt tttgtacgaa caacaatgac
     1321 attgatatct ttgtcgtagt caattgattt gataatatca ttttgcttga gcatgttttg
     1381 atttaataaa taaccaacag ctaagtatct tgggtagtca ggaatggaca ttaatgttac
     1441 ta
//
LOCUS       DTVV01000121            1155 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1318861, whole genome shotgun sequence.
ACCESSION   DTVV01000121 DTVV01000000
VERSION     DTVV01000121.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1318861"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="EYO86_01480"
     CDS             <1..650
                     /locus_tag="EYO86_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018997412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIC41740.1"
                     /translation="YENGKPKKVASVVVSTQHKEGLSPGHIKDLVRPIVYDCIPENLI
                     NELKDKDFYVNPTGNFVIGGPDGDSGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDR
                     SAAYAARYLAKNIVAANLAEECVIQLSYAIGVSKPLSIYINTNGTSQVDETKIENLIP
                     ELFDLSPRGIREHLQLSNPIYVPTSAYGHFGRTPTMQGHFTWEKTDLVESFKNAF"
     gene            661..1128
                     /gene="tsaE"
                     /locus_tag="EYO86_01485"
     CDS             661..1128
                     /gene="tsaE"
                     /locus_tag="EYO86_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIC41741.1"
                     /translation="MYKIIECKCDLKQLNKFSQKFAQKAAVGDIIFLKGELGVGKTTF
                     ARFFINALFDNLSLQKPQSIKSPSFPIMINYSLLKYEIFHYDLFRLKSINEISEIDIF
                     ENLHGNIIIVEWPELIMNHLSVKDFYLIELELIDLSKRHLKMFHSKKKRFNEF"
BASE COUNT          418 a          161 c          197 g          379 t
ORIGIN      
        1 tatatgaaaa tggtaaacct aaaaaagtag catcagttgt ggtgtcaact caacataaag
       61 aaggcttgtc accaggacac attaaagatt tggttagacc tattgtatat gattgcattc
      121 ctgaaaattt aattaatgaa ctaaaagata aggactttta tgttaatcct actggaaatt
      181 ttgtaattgg gggacctgat ggggactctg gtttgacagg aagaaaaatt atcgttgata
      241 cttatggagg agctgcacct catggcggag gcgcattctc aggaaaagat ccatcaaaag
      301 tagatcgctc ggcagcttat gcagctagat atttggctaa aaacattgta gcagcaaatt
      361 tagcagaaga gtgtgttatt cagctatctt atgcaattgg tgtttccaaa cctttgtcaa
      421 tttatataaa tactaacggc accagccaag tggatgaaac aaaaatagag aatctaattc
      481 cagaactttt tgatttaagc ccacgcggta ttcgtgaaca cctacaacta agtaatccaa
      541 tttatgttcc aacatcagca tatggccact ttggaagaac tcctactatg cagggtcatt
      601 ttacttggga aaaaactgat ttagttgaaa gttttaaaaa tgccttttaa cttaacttaa
      661 ttgtacaaaa taattgaatg taaatgtgat ttaaagcaat taaataaatt ttctcaaaaa
      721 tttgctcaaa aagccgcagt gggagatatt atttttctaa aaggtgaatt aggagtagga
      781 aaaacaactt ttgcacgttt ttttattaat gctttgttcg ataatttatc attacaaaaa
      841 cctcaaagta taaaaagccc ctcttttcct ataatgatta attattcatt attaaagtat
      901 gagatttttc attacgatct attcagactg aaaagtataa atgaaatatc tgagatagat
      961 atatttgaaa atttgcatgg taatataata attgtcgaat ggccagaatt aatcatgaat
     1021 catctcagtg ttaaggattt ttatttgatt gaattagagt tgatcgattt atcaaaaaga
     1081 catttaaaaa tgtttcattc aaaaaagaaa agatttaatg aattttaatt ttttagataa
     1141 attatctcct gaata
//
LOCUS       DTVV01000122            1128 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1321237, whole genome shotgun sequence.
ACCESSION   DTVV01000122 DTVV01000000
VERSION     DTVV01000122.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1321237"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /gene="infB"
                     /locus_tag="EYO86_01490"
     CDS             <1..1120
                     /gene="infB"
                     /locus_tag="EYO86_01490"
                     /inference="COORDINATES: protein motif:HMM:PF10662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIC41742.1"
                     /translation="NIDADTAEIIAGEFGHKVKRVADSDYDVEFKKEIDKKEDLVDRA
                     PVVTIMGHVDHGKTTLLDAFRSSNIVSKEHGGITQHIGAYRVKTKSKKFITFIDTPGH
                     EAFTAMRARGSKVTDIVVLVVAADDGVKETTIEAINHAKAADVPIIVCINKIDLNESN
                     PQNVKNQLMEYEILSEEMGGKNLFVEVSAKSKTNLDKLVEAILLQAEILNLKANPSKK
                     AAGIIVESKILQGKGSTATVLIQEGSLKVGDFFVAGPSYGKVRAMNDDLGKKITEAKP
                     AVPVEIIGITGVPIAGDYFKVVENETKGKEIANYRLHKSKIKQTEPLTKESLEKQLSD
                     KSNEKIKELGLILKSDVQGSSAVSVHCRILRQVRCTGL"
BASE COUNT          444 a          146 c          223 g          315 t
ORIGIN      
        1 aaatatagat gcagatacag ctgaaattat tgccggtgaa tttggacata aagttaagag
       61 agttgctgat tctgattatg atgttgagtt taaaaaagag attgataaga aggaagattt
      121 agtagataga gccccagttg tgacgattat ggggcatgtt gatcatggaa aaacaacatt
      181 attagatgca tttcgatcta gcaacattgt ttctaaagaa catggtggta taacgcaaca
      241 tattggagca tatagagtta aaacaaaatc taaaaaattt ataacattta tcgatacacc
      301 tggacacgaa gcatttactg caatgagagc acgtggatca aaagtaactg atattgtggt
      361 tttagttgtt gctgcagacg atggagttaa agaaacaact atagaagcta tcaatcatgc
      421 taaagctgct gatgtaccaa ttattgtttg tataaataaa attgatttaa atgaatcaaa
      481 tccacagaat gtaaaaaatc aattaatgga atatgaaatt ctttcagaag aaatgggtgg
      541 aaagaattta tttgttgaag tatctgcaaa gtcaaagaca aatttagata aattagtaga
      601 agcaatttta ttacaggcag aaatattaaa tttaaaagct aatccatcaa agaaagcagc
      661 cggcattatt gttgagtcaa aaattcttca aggaaaagga tctacggcaa ctgttttgat
      721 tcaagaaggg tcattaaaag ttggcgattt ttttgtagct ggcccctctt acggaaaagt
      781 aagagcaatg aatgatgatt tgggaaagaa aataactgaa gcaaagccag cagtaccagt
      841 tgaaataatt ggtataacag gcgtaccaat tgcaggagat tattttaaag ttgttgaaaa
      901 tgaaacaaaa ggtaaggaaa ttgcaaatta tagattacat aaatctaaga ttaaacaaac
      961 tgaaccactt acaaaagaaa gtttagaaaa acaacttagt gataaaagca atgaaaaaat
     1021 taaagaatta ggtttaattt taaaatcaga tgttcaagga tcatccgcag tatcagtaca
     1081 ctgccgaatt ctgcgccaag tacgatgcac cggcctttga cccggatt
//
LOCUS       DTVV01000123            1042 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1323605, whole genome shotgun sequence.
ACCESSION   DTVV01000123 DTVV01000000
VERSION     DTVV01000123.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1323605"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(39..992)
                     /locus_tag="EYO86_01495"
     CDS             complement(39..992)
                     /locus_tag="EYO86_01495"
                     /inference="COORDINATES: protein motif:HMM:PF05426.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41743.1"
                     /translation="MPKQRAASSVEVVGHGTQYTIYNMPNPFDGDILWMDGQVSGWLR
                     TGDNAYLKTLRDWMLASANAGSLTKLVPDPDKYFFEDPLFNLRFTLKTTFVAYDLLRQ
                     TKFLKPEENQKILDWLAPIVKNSDRRSCESKWKCKPDSRPGEHWTLHDYTTLMLWGAV
                     SGDNYYFQRGTKMYVKSLKSLSSKGASKEVKKKKHRALQKQNENVGYFVMLAEIAANQ
                     GYDLYSVEQKKKSLFTAFEYLIWGIEHPDKIYKKRTQDLLFLQKQMHSDKTPAWFEPF
                     LRRWPDHPLSKKFSTLIKTTSRPMFGATYGGNLSCFFYRKK"
BASE COUNT          331 a          194 c          159 g          358 t
ORIGIN      
        1 aaccaacaaa cccagaacca agatccctaa aagttttttc attttttcct ataaaaaaaa
       61 caagataaat tccccccata cgtggcacca aacataggtc gtgaagttgt tttaatcaat
      121 gtcgaaaatt ttttactcaa tggatggtca ggccatctac gaagaaaggg ctcaaaccaa
      181 gctggagtct tatcgctatg catttgtttt tgtaaaaaaa gtaaatcttg tgttcttttc
      241 ttgtaaattt tatcaggatg ttcaatgccc catattaaat attcaaaagc cgtgaaaaga
      301 gatttctttt tttgttctac actgtaaaga tcatatcctt gattagctgc aatttcagct
      361 aacattacaa aatatccgac attttcattt tgcttctgta aagcacgatg cttctttttt
      421 ttaacttctt ttgaagcacc tttagatgat aaacttttta aagatttaac gtacattttg
      481 gtacctcgtt gaaaataata attatcaccc gatacagcac cccatagcat taatgtggta
      541 taatcgtgca gggtccaatg ttcacccggt cgagaatctg gtttacattt ccattttgat
      601 tcacaactac gtctatctga atttttaaca attggtgcca accaatctag aatcttttga
      661 ttttcttcag gttttaaaaa tttagtttgt ctaagtaaat cgtatgctac aaatgtagtt
      721 tttaatgtaa aacgtaaatt aaaaagtgga tcttcaaaaa aatatttatc tggatcaggt
      781 actagttttg ttaaggatcc tgcgtttgct gacgccaaca tccaatcgcg caaagttttt
      841 aaatacgcat tgtctccagt tcttaaccag ccactaactt gaccatccat ccataaaata
      901 tcgccatcaa atggattggg catattatag attgtatatt gcgttccgtg acctactacc
      961 tcaacagaag atgccgctcg ttgttttggc aaagtataaa aattagtggt acaaatttta
     1021 ttagaaaatt ctggatcaga tt
//
LOCUS       DTVV01000124            1073 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1326748, whole genome shotgun sequence.
ACCESSION   DTVV01000124 DTVV01000000
VERSION     DTVV01000124.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1326748"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="EYO86_01500"
     CDS             <1..1000
                     /locus_tag="EYO86_01500"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020169324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HIC41744.1"
                     /translation="KILKEVAEKYQFKIQFHEFDFASCNFYAKHGKMLPDNWKEKIGS
                     HDAIFFGAVGMPDQVPDHVSLWGSLLKFRRDFDQYINLRPVKLMPGVPCPLINKKPGD
                     IDMLIVRENTEGEYSSVGGRMYQGTDREIAIQETIMSKKGIDRVQKFAFELAKNTKRK
                     KLTSATKSNGISITMPFWDERFEENKKNYPDVKTDQYHIDILAARFVLTPEWFDVIVA
                     SNLFGDILSDLGPACTGTIGIAPSANINPEKKFPSLFEPVHGSAPDIAGKGIANPIGQ
                     IWSGAMMLDYLGEKEAANSIITSIEKTLSDKKSRTKDLNGDSNTSQCAETVLNNLT"
BASE COUNT          403 a          154 c          198 g          318 t
ORIGIN      
        1 taaaatatta aaagaagtag cagaaaaata tcagtttaag attcaatttc acgaatttga
       61 ttttgcttca tgtaattttt atgcaaagca cggaaagatg cttccagata actggaaaga
      121 gaaaattggt agtcatgatg caattttttt tggggcggtt ggaatgccag accaagtgcc
      181 agatcatgtt tctttatggg gttctttatt aaagtttaga agagattttg accaatatat
      241 aaatttaaga cctgttaagt taatgccagg agttccttgt ccattaataa ataaaaaacc
      301 aggagatatt gacatgctta tagttaggga aaatacagag ggagagtact catctgttgg
      361 aggaaggatg tatcagggaa cagatagaga aattgcaatt caagaaacca taatgtcaaa
      421 aaaaggtata gatcgtgttc aaaaatttgc gtttgaatta gctaaaaata caaaaagaaa
      481 aaaactaaca agcgcaacca aatcaaatgg tatttcaatt acaatgcctt tttgggatga
      541 aagatttgaa gaaaacaaaa aaaattatcc tgatgtaaaa actgatcaat atcatataga
      601 tattttagca gcaagatttg tgcttacacc cgaatggttt gatgtaattg ttgcttcaaa
      661 tttatttggt gatattttat ctgatcttgg acctgcctgt actggaacaa tcggcattgc
      721 cccatcagca aatattaatc cagaaaaaaa atttccgtct ttatttgaac cagttcatgg
      781 ctcagctcct gatattgcag gaaaaggtat agcaaatccc ataggtcaaa tttggtctgg
      841 agcaatgatg ttagattatc ttggtgaaaa agaagctgcc aattcaataa ttacttcaat
      901 tgaaaaaact ttatctgata aaaaaagtag aacaaaagat ttaaatggag acagtaatac
      961 ctctcaatgt gctgaaacag ttttgaataa tttaacttaa aggatttaaa acaaaatatt
     1021 aaatgctaag gggcattccg tctgacaggt gcaattaacc cctcaccccg aaa
//
LOCUS       DTVV01000125            2075 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1328865, whole genome shotgun sequence.
ACCESSION   DTVV01000125 DTVV01000000
VERSION     DTVV01000125.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1328865"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(113..934)
                     /gene="mreC"
                     /locus_tag="EYO86_01505"
     CDS             complement(113..934)
                     /gene="mreC"
                     /locus_tag="EYO86_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIC41745.1"
                     /translation="MLSKNRFTSATKKKTFSGVYLSVLFVLTFLLILFNKTDYYIANK
                     IKHVGIDIISPITKIISSPVTIVTNIGLRLNNIRYLEGENLKLKQEIIRLKKWQTLAI
                     KNTGENRVFKRLLNSTSNEIEIIKTASVINQTTNLYVNAITINAGLEHKVKEDLAVIN
                     EKGLVGKTITSSNQNSKVLLINDQNSSVPVKNLSDKSYSIVRGTANGKYLISAFIKDE
                     TLPKVGDLLVTSGNAKIFPRDILVGKVILVREDDYLAIPYVDFKNLGYVQVIKSK"
     gene            complement(939..1979)
                     /locus_tag="EYO86_01510"
     CDS             complement(939..1979)
                     /locus_tag="EYO86_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIC41746.1"
                     /translation="MVIEKLFNIFAKDMAIDLGTANTLVYVKGEGVVLNEPSVVAKIE
                     ENGKIKVLAFGNEAKLMLGRTPGKIQAIRPMKDGVIADFEVAEQMIKHFIQKANKGRT
                     ISNPQIVICVPSGATNVERRAIRESADAAGARRVFLVEEPVAAAIGAGLPVGEPTGSM
                     VVDIGGGTTEVAVLSLGGVVLSTSIRSAGDKFDEAITEYMRSTHKLAIGETTAERMKK
                     EIGTACPPDDGEGDTMHVKGRDLISGLPKEIAISQRQIAEALAEPVATIIDTIKRALE
                     QTPAELAADIVERGIMLTGGGSLLGNLDKVLRRSTSLPVSIAEDPLLCVVLGTGRALE
                     QKDKLGDIFASE"
BASE COUNT          654 a          399 c          292 g          730 t
ORIGIN      
        1 aaattatatt taaaattgga aataatggca cgccattaac agttaatgaa aaaaaaatga
       61 ttaataaaat attataaatg ataaaagaat tataatactg cgataagtgc atttattttg
      121 attttataac ttgcacataa ccaagatttt taaaatcaac ataaggaata gccaaataat
      181 catcctcccg tacaaggatt actttaccta ctaaaatatc acgtggaaat atttttgcat
      241 ttccgctagt aactaaaaga tcacctactt ttggcagtgt ttcatcttta ataaaagcgc
      301 taattaaata cttcccatta gccgtacctc gaacaattga gtaagattta tcagataaat
      361 tttttacagg aactgaagaa ttttgatcat taattagtaa aacttttgaa ttttgattcg
      421 aagaagtaat agtttttcca accaatcctt tttcattaat tacagctaaa tcttctttaa
      481 ccttgtgttc aagacctgca ttaatagtta tagcattgac atagagatta gttgtttgat
      541 taataactga agctgttttt ataatctcta tttcattgga tgtagaattt aataaacgct
      601 taaaaactct attttctccg gtattcttaa tggcaagggt ttgccatttt tttaatctaa
      661 taatttcttg ttttagtttt aaattttctc cttctagata acgaatatta tttaagcgca
      721 atccgatatt ggtaactatt gtaacagggg aggaaattat ttttgttatc ggagatatta
      781 tgtctatacc aacgtgtttt atcttatttg caatataata atcagtttta ttaaataaga
      841 taagcaaaaa agttaaaaca aataaaacag ataaataaac accgctgaaa gttttcttct
      901 ttgtagcaga ggtaaaccta tttttagata acatttaatt attcagatgc aaaaatatca
      961 cctagtttat ctttttgctc aagagctctc cctgtgccca gaaccacgca cagtaatgga
     1021 tcttcagcaa tagaaacggg taaactagtt gatctcctta aaactttatc aagattgcct
     1081 aatagagagc ctccaccagt aagcataata cctctttcaa caatatcagc tgccaattct
     1141 gcaggcgttt gttccaaggc tctttttata gtgtcaatta tcgttgctac aggttctgcc
     1201 aaagcctcag caatttgcct ctgggaaatg gcaatttctt taggaagtcc ggaaattaaa
     1261 tccctcccct taacatgcat agtgtcacct tcaccatcgt caggtggaca agcagtacct
     1321 atttcttttt tcattctttc agcagttgtc tctccaattg ctaatttatg ggttgatcgc
     1381 atatactctg taatcgcttc atcaaattta tctccagcag atctaatgga tgttgataaa
     1441 actactcctc ccaatgatag aacggcaacc tcagtagttc cgcctcctat atctacgacc
     1501 attgatccag taggctcgcc cactggtaaa cccgctccta ttgcagcagc aactggttct
     1561 tcaactaaaa aaactcttct agcgccagca gcatccgcag attcacgaat tgctcttcgc
     1621 tcaacatttg tagctccaga aggcacgcat ataacgattt gtggattgga tattgttcta
     1681 cctttattcg ctttttgaat aaaatgtttt atcatttgtt ctgcaacctc aaaatccgct
     1741 ataactccat ctttcattgg cctaatggct tgtatcttgc ctggggttct cccaagcatt
     1801 agttttgctt cattaccaaa tgccagcact tttatttttc cattttcttc tatcttagct
     1861 acaaccgatg gttcatttaa tactactcct tcacctttaa catacacaag ggtattggct
     1921 gtcccaagat cgattgccat atctttagca aatatattga ataatttttc tataaccatt
     1981 aatttacctt ttatacccct tctattctaa gttctgaaac ttgatgatta attgatttag
     2041 attttttaaa tatcaaacga tttaaagcat taata
//
LOCUS       DTVV01000126            1973 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1329526, whole genome shotgun sequence.
ACCESSION   DTVV01000126 DTVV01000000
VERSION     DTVV01000126.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1329526"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            44..394
                     /locus_tag="EYO86_01515"
     CDS             44..394
                     /locus_tag="EYO86_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41747.1"
                     /translation="MDLLSILRWYEPKLAAASCALARQQEYQTDRMSASVVSPHAAVN
                     ALVRGAIAKAFVGEVLWREVWERPDREPRPPEQVYDRLYEALGRLSEWDDARASFESA
                     LVETTDETDTHLRS"
     gene            complement(441..911)
                     /locus_tag="EYO86_01520"
     CDS             complement(441..911)
                     /locus_tag="EYO86_01520"
                     /inference="COORDINATES: protein motif:HMM:PF04519.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HIC41748.1"
                     /translation="MFKTLRGVEEKKLNYNGRSHIGETMQFEGDLRSSGSLDVAGLMN
                     GNIFVSDMTITEAGSVRGSIEAASIEVNGHVEGKITADVVIVGKNAVIKGDIFFKNTL
                     KTEEGADIDGYIKRVSKGKSDTEEDIAIEEIVEREDSTKSKPRVVHVGQHKEAV"
     gene            complement(1035..>1973)
                     /gene="rodA"
                     /locus_tag="EYO86_01525"
     CDS             complement(1035..>1973)
                     /gene="rodA"
                     /locus_tag="EYO86_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIC41749.1"
                     /translation="ILFIIVSFFRIKTWYKSAYLFYFAVLILLFAVDIFGVTASGSKR
                     WISLFFINLQPSELMKIALIIFLARYYYKIPTENVTNLKYIITPFFAIFVPVLLVAEQ
                     PDLGTAVLILIGGLAVIWLTGFRIKLFLYSCFFLICLLPVGISFLKPYQKTRILSFFD
                     PERDALGAGYQIIQSKIALGSGGIFGKGFLQGSQSYLDYLPEKHTDFIFTLFSEEFGF
                     VGSTALLLVYVMIIYRITVIGRQSRNNFAKLYCFGFATAFFAYVAVNMSMVLGLLPIV
                     GAPLPIMSYGGSSMLSVMIGLGFVMSCKIYQQEDVN"
BASE COUNT          630 a          424 c          350 g          569 t
ORIGIN      
        1 agcggatact gggtgccgtc gagggcgaag acgatgcggg agcttggatc ttctaagcat
       61 tctacgctgg tacgagccca agcttgccgc cgcttcttgt gcgctggccc gccagcagga
      121 gtaccaaacc gaccggatgt cggcctcagt ggtctcgccg cacgccgcgg tcaatgcgct
      181 ggtccgcggg gccattgcca aagcctttgt cggcgaggtc ttatggcgcg aggtctggga
      241 acgccccgac cgggagccgc ggccaccgga gcaggtctac gaccggctct acgaggcgct
      301 gggccggctt tcggaatggg acgacgcacg ggcgagtttc gagtccgcgc ttgtcgagac
      361 gactgatgag accgacaccc atcttcgctc atagtatcgc tagttatatg ttatttaaga
      421 tttaaatatc gggtaacacg ttatacagct tctttatgct gccctacatg aacgactcta
      481 ggtttagatt tcgttgaatc ttctctttcc actatttctt caattgcaat atcttcttct
      541 gtatctgact tccctttgct tactcgtttt atataaccat caatatcagc gccttcttct
      601 gtttttaaag tatttttaaa aaaaatatcg ccttttatca ctgcattttt tccaactatt
      661 acaacatcag ctgtaatttt tccctctaca tgtccgttaa cttctatact tgctgcttct
      721 atcgaacctc ttactgatcc agcttcggtt atcgtcatgt ctgaaacaaa tatgttgcca
      781 ttcatcagcc ccgcaacatc taatgatcct gatgatctta aatcgccttc aaactgcata
      841 gtctcaccaa tatgtgatcg accattgtaa tttaattttt tttcttcaac tcctcgtaaa
      901 gttttaaaca tttgttttag ttaagcacaa aaagtcaatt ttttaaaact aaaaagaaaa
      961 aaatttcgcg atattgattg gaatcaaaag taaataatac aatctcaagt tgttttataa
     1021 aagcccctaa gtatttaatt tacatcctcc tgttgataaa ttttacaact catcacgaaa
     1081 cccaagccaa tcattaccga aagcatcgat gagcccccat atgacataat aggaagtgga
     1141 gctcctacaa tcggcagtaa accaagtacc attgacatat taactgcaac ataagcaaaa
     1201 aatgccgtgg caaaaccgaa gcagtataac tttgcaaaat tatttctcga ctgacgacca
     1261 attacagtaa ttctgtatat aatcataacg taaacaagta ataatgctgt agagccgaca
     1321 aaaccaaatt cttcggaaaa taaagtgaaa atgaaatctg tgtgcttttc aggtaaataa
     1381 tccaagtaac tctggcttcc ttgcaggaaa ccttttccaa aaatgcctcc tgaacccaag
     1441 gcaatttttg actgaataat ctgatagccg gcccctaaag catctctttc gggatcaaaa
     1501 aaagatagga ttcttgtttt ttgatacggt tttaaaaatg atattccaac tggaagtaag
     1561 caaattaaaa agaaacatga gtacaagaaa agttttattc tgaaacccgt taaccaaata
     1621 acggccaaac cgccaattaa aattagcact gctgttccca aatccggttg ttctgcaact
     1681 aataaaacag gaacaaatat agcaaagaaa ggtgtaataa tatattttaa gtttgtgaca
     1741 ttttctgtag gaattttata atagtatctt gctaaaaaaa taattaaagc aattttcatt
     1801 aattcggaag gctgaagatt aataaaaaat aaactaatcc aacgttttga tcctgaggcg
     1861 gtaacgccaa aaatatcaac ggcaaacaat aagattaata ctgcaaaata aaataaataa
     1921 gcagatttat accaagtctt aattctaaaa aaagaaacta ttataaaaag tat
//
LOCUS       DTVV01000127            3526 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1334184, whole genome shotgun sequence.
ACCESSION   DTVV01000127 DTVV01000000
VERSION     DTVV01000127.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1334184"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /gene="dxs"
                     /locus_tag="EYO86_01530"
     CDS             <1..1727
                     /gene="dxs"
                     /locus_tag="EYO86_01530"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIC41750.1"
                     /translation="TPKDKIIWDVGHQAYPHKILTGRKHQIETLRKENGLYGFVKRSE
                     SEYDTFGTAHSSTSISAGLGIKIALELKKDNSKVVCVIGDGALSAGMAYEALNNAGAM
                     SKELIVVLNDNDMSIDQPVGAMSSYLSKLLSSNSYSNIRSIIKKISNKFPDSFQKTLY
                     RAEEFSKGFLSGGTLFEELGFFYLGPIDGHKFDHLIPVLRNIKLNKINKPIFLHVITK
                     KGKGYKPAEESLDKFHGVNKFDIVTGSSLSKVKKKSYSQVFGETLSDEALKDEKIVAV
                     TAAMASGTGLNVFSQRHPKKFFDVGIAEQHAVTMCAGLATEGFKPFAAIYSTFLQRAY
                     DQIVHDVAIQKLPVRFAIDRAGQVGADGPTHAGSFDIAFLSTLPNFIIMAASDETELI
                     RMIKTSILIDDRPSSFRFPRGSVIGMSNESNLSPLEVGKGRMIKEGHSVALINLGARL
                     EACKEALDILSPQGCKPTLMDARFAKPLDTSLLDQIFNNHEFVITIEEGSIGGFSSSV
                     LDYIHNKKNSPTKSIIKNIIFPDKFVNHNTPENQYKEIGMNAESIANKILSLMSSEVV
                     HFSNYTKK"
     gene            1731..2468
                     /locus_tag="EYO86_01535"
     CDS             1731..2468
                     /locus_tag="EYO86_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIC41751.1"
                     /translation="MLKKRVDLLLVDKNIVESRTKAKAMIMAGQISINGKKVLKSGET
                     YEEDVNIHVTKLHPQWVSRGALKLCHAIQNFNIEIANLVCLDLGASTGGFTEVLLSKN
                     VKKIYAVDVGSNQLHEKLKKDPRIVNLSKTNARYLNKDIIHDIIDIIVCDVSFISMKK
                     VIQPSLQFLDEENGAVIGLIKPQFESKKNEIKRGGVIVDPAIHKRICHEYREWFTSTC
                     NMKVQRIIPSPIKGPKGNIEFLIYAKK"
     gene            complement(2469..3515)
                     /gene="aroC"
                     /locus_tag="EYO86_01540"
     CDS             complement(2469..3515)
                     /gene="aroC"
                     /locus_tag="EYO86_01540"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIC41752.1"
                     /translation="MFSFTTWGESHGKAIGCVVDGVPSQISLSEKDIQVYLDRRRPGQ
                     SKFTTQRQEKDQVEILSGVFESLTTGHPISLIIHNEDQRSKDYSDIKDIFRPGHADYT
                     YWAKYGIRDYRGGGRSSARETAMRVAAGSIARKIIEHKIKNNFKITGALVQIGSSKID
                     YENWNDDFILKNDFFCPDKKIIIKWEEIISKARKDGSSLGAIIELRCQGMPAGLGEPV
                     YDKLDADLAKALISINAVKGVEIGNGFRSVTESGISNVDEMRIQNGKPIFLSNNNGGI
                     LGGITTGQDIVVRFCVKPTSSIISAKKTINTHLEETTISTKGRHDPCVGIRAVPVGEA
                     MMATTLADHCLRYR"
BASE COUNT         1190 a          574 c          606 g         1156 t
ORIGIN      
        1 atactcctaa agataaaatt atttgggatg tgggtcatca agcctaccct cataaaatat
       61 tgactggtag aaaacatcaa attgagacgt tgcgtaaaga aaatggtctt tatggttttg
      121 ttaaaagaag cgaaagtgaa tacgacacat ttggtacagc acatagttcc acctctatat
      181 cagctggttt aggaattaaa attgctctag aattaaaaaa ggataattca aaagttgttt
      241 gtgtaatagg tgatggagcc ttgagtgcag gaatggcata tgaagcatta aataatgcag
      301 gagctatgag taaagagttg attgttgtac ttaatgacaa tgacatgtct attgatcaac
      361 cagttggagc tatgagttct tatttgtcca aactgttatc atcaaattct tattcaaata
      421 tacgatcaat aattaaaaaa atatcaaata aattcccaga ttcctttcaa aagactttat
      481 atcgtgcaga agaattctct aaaggatttc tatctggagg aacattattt gaagaattag
      541 gtttttttta tttagggccc atagacggtc ataagtttga tcatttaatt ccagtgctta
      601 gaaatattaa attaaacaaa ataaacaagc ctattttttt gcatgtgatt acgaaaaaag
      661 gtaagggata taagcctgcg gaagaatctt tagataagtt tcatggtgta aataaatttg
      721 acattgtaac tggctcttca ttgtccaagg ttaagaaaaa atcatattca caagtgtttg
      781 gggagacctt gtcagatgaa gctttgaaag atgagaaaat tgttgctgta acagcagcta
      841 tggcttctgg aacgggctta aatgtttttt cgcagcggca tccaaaaaaa ttttttgatg
      901 taggaatagc tgaacagcat gctgttacta tgtgtgcagg cttggcaaca gaaggtttta
      961 aaccttttgc ggcaatatat tcaacttttc ttcaaagagc ttatgatcaa atagtgcatg
     1021 acgtagctat ccaaaaattg ccagtaagat ttgccataga tagggcgggt caagtgggag
     1081 ctgatggacc tacacatgcc ggatcatttg atatagcctt tctatctaca ttgccaaatt
     1141 ttattattat ggcagcttca gatgaaactg agttaatccg catgataaaa acttctatat
     1201 taattgatga tcggccatcc tcctttcgtt tcccaagggg atctgtgatt gggatgagta
     1261 atgaaagcaa tttatctccc cttgaagttg gcaaaggacg tatgattaaa gaaggtcatt
     1321 cagtggcttt gataaactta ggggcaagat tggaagcatg taaggaagcg ctagacatac
     1381 tcagtccgca gggatgtaag ccaacattaa tggatgcacg atttgccaaa cctttagata
     1441 catcgctatt ggatcaaatt ttcaataatc atgagtttgt aattacaatt gaagagggct
     1501 ctatcggggg atttagttct tcagttcttg actatattca taacaaaaaa aattcaccaa
     1561 ctaaatctat aataaaaaat ataatatttc ccgataagtt tgttaatcat aacacccctg
     1621 aaaatcaata taaagaaata ggtatgaatg ctgagtctat tgccaataaa atactatctt
     1681 taatgtcttc tgaggtagtt cactttagta attatacaaa aaaatagtat attttgaaaa
     1741 aaagagttga tctactttta gttgataaaa atattgttga gtcacgtaca aaagcaaaag
     1801 ctatgattat ggctggacaa atttcaatta atggaaaaaa agttttgaaa agtggtgaaa
     1861 cgtatgaaga agatgttaac attcatgtaa caaaattaca tcctcagtgg gtatcacgag
     1921 gagcgctgaa gttgtgtcat gcaattcaaa atttcaatat tgaaatagct aacttagttt
     1981 gtctagattt gggcgcatct actggtggtt ttacagaagt gttattgtca aaaaatgtaa
     2041 aaaaaatata tgctgttgat gttggatcta atcaattaca tgaaaaatta aaaaaagatc
     2101 ctagaattgt taatttatca aaaacaaatg ctagatattt aaataaagat attatccatg
     2161 acataattga tattattgtt tgtgatgtaa gctttatcag tatgaaaaaa gttattcaac
     2221 catccctcca atttcttgac gaagaaaatg gagcagtcat tggtctaatt aaacctcagt
     2281 ttgaatctaa aaagaatgag ataaaaagag gaggtgtaat tgttgatcct gcaattcata
     2341 aaagaatatg tcatgaatat agagaatggt ttacttctac atgcaatatg aaagtccaaa
     2401 gaattatacc aagtccaata aagggcccta aaggaaatat tgaattttta atatacgcta
     2461 aaaaataatt atcggtaacg caagcagtga tcggcaagtg tggttgccat catagcctca
     2521 cctacaggaa cagccctaat acctacacat gggtcatgtc ttccttttgt tgaaattgtt
     2581 gtttcttcta aatgtgtatt aattgttttc ttagcactta ttattgagct tgttggtttt
     2641 acacaaaaac gaactacaat atcttgtcct gtggttattc cacccaaaat acctccatta
     2701 ttattagaaa gaaatatagg tttgccattt tgaatacgca tttcatcaac atttgagatt
     2761 ccagattccg taacacttct aaatccattc cctatttcta cccccttaac agcattaata
     2821 cttattaatg ctttggccaa atcagcatct aatttatcat aaacaggttc ccccagccct
     2881 gcaggcattc cttggcatct taattcaatt atagctccta gagaagatcc atccttacgt
     2941 gcttttgaaa ttatttcttc ccatttaatt attatttttt tatctgggca aaaaaaatca
     3001 ttttttaaaa taaaatcatc attccagttc tcatagtcta ttttgctact accgatttgt
     3061 actaaagctc cagtgatttt aaaattattt tttattttat gttcaataat ttttcttgca
     3121 atactaccag ctgcaacccg catagctgtt tctctggcac tagatcttcc accacctcta
     3181 taatcacgaa taccatattt tgcccaatac gtatagtcag catgacctgg gcgaaagata
     3241 tccttaatat cggaataatc tttagatcgc tgatcttcat tatgaattat taaagagatt
     3301 ggatgacccg ttgttagact ttcaaaaact ccagataata tttctacctg atctttttct
     3361 tgtcgttgtg tggtaaattt tgattgacct ggccgacgtc tatctaaata aacttgaata
     3421 tctttttctg ataaagaaat ctgcgatggc actccatcaa caacacatcc tatagctttt
     3481 ccatgacttt ccccccatgt tgtaaaacta aaaatttttc ctgatg
//
LOCUS       DTVV01000128            1419 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1339045, whole genome shotgun sequence.
ACCESSION   DTVV01000128 DTVV01000000
VERSION     DTVV01000128.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1339045"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="EYO86_01545"
     CDS             <1..397
                     /locus_tag="EYO86_01545"
                     /inference="COORDINATES: protein motif:HMM:PF08534.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIC41753.1"
                     /translation="KSSAITSKTILFFYPKAMTPGCTVEVNEFQSNFNKFQKLGFTII
                     GCSKDSIENNIKFAEKYKLRYLLGSDLTNVCEKLGIWIEKSMYGKKYFGIDRSTFMID
                     SKGKLFKQWNNVKVKDHVKEVLEIAKNCP"
     gene            419..>1419
                     /locus_tag="EYO86_01550"
     CDS             419..>1419
                     /locus_tag="EYO86_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC41754.1"
                     /translation="MLKSNYSSHLYGFTDMKSLNERGPIIISHGKGIYVYDIHNKKYL
                     DANSGLWNSCAGFDHPGIIEVAQKQYAKFGGYHSLFGRLSEETIQLAEKIIEVSPFNE
                     GKVFFCNSGSEANDTTVKMLWMLNKRRGHPKKRKIITRINAYHGVTLGASSMTGKSYI
                     EEFGMPLADFIHADCPHFWKFGLPNESEEDFSKRMAKNLEDLILKEDADTIAGFFAEP
                     VMGAGGVIPPSEGYFDLIQPILKKYSIPFIADEVICGFGRTGNLWGSDTYNLKPDIII
                     SSKCLTAGYFPMGAVIVNKDFADDFTSVSEEAEEFPHGFTSGGHPVGCAIALKAIDV"
BASE COUNT          500 a          190 c          267 g          462 t
ORIGIN      
        1 aaaatcttca gcaataacat ctaaaactat tttatttttt tatcctaaag ctatgacacc
       61 tggctgcact gtagaagtaa atgaatttca atctaatttt aacaagtttc aaaaacttgg
      121 ttttacaatt attggatgtt caaaagattc aatagaaaat aatattaaat ttgccgaaaa
      181 atataagtta agatatttgt taggatcgga cttaacaaat gtttgtgaaa aattgggaat
      241 ctggatagaa aaatctatgt atggaaaaaa gtattttgga atagatagat ctacatttat
      301 gattgatagt aagggaaaac tatttaaaca atggaataat gttaaagtta aagatcatgt
      361 taaggaagtt ttagaaattg caaagaattg cccataattt acaaaaaaga aacagcatat
      421 gttaaaaagt aattactcct ctcatttata tggtttcaca gatatgaaat cacttaatga
      481 gagaggcccc attatcattt ctcacggtaa aggtatttat gtttatgata ttcataataa
      541 aaaatacctt gatgctaact ctggtttatg gaattcatgt gccgggtttg atcatcctgg
      601 aattatagaa gttgcacaaa aacaatatgc taaatttgga ggataccatt ctttgtttgg
      661 taggttgtca gaagaaacaa ttcaattggc tgaaaaaatt attgaagtct ctccgtttaa
      721 tgaaggtaaa gtgtttttct gcaactcagg atccgaagca aatgatacga cagtaaaaat
      781 gctctggatg cttaataaaa gaagaggcca tcctaaaaaa agaaaaatta ttacaaggat
      841 caatgcttac catggagtga cattgggagc aagttcaatg acaggcaagt cttatattga
      901 agagtttgga atgcctttag cagattttat tcatgctgat tgcccacatt tttggaaatt
      961 tggattacct aatgaatctg aagaagattt ctctaaacgc atggcaaaaa atttagaaga
     1021 cttaatatta aaagaagatg cagataccat agctggtttt tttgcagaac ctgttatggg
     1081 agcagggggt gtaattccgc cttctgaagg ttattttgat ttaattcagc caatactcaa
     1141 aaaatacagt attcctttta ttgctgatga agtcatatgt ggatttggta gaacaggaaa
     1201 tctttgggga agtgatactt ataatttaaa acccgatata attatttctt ccaaatgttt
     1261 aactgctggt tattttccca tgggagctgt tatagtaaat aaagattttg cggatgattt
     1321 tactagtgtt tcagaggaag cagaagagtt cccccatggt tttacatcgg gagggcatcc
     1381 cgttggatgc gctattgccc taaaagctat tgatgttat
//
LOCUS       DTVV01000129            1154 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1339768, whole genome shotgun sequence.
ACCESSION   DTVV01000129 DTVV01000000
VERSION     DTVV01000129.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1339768"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            24..248
                     /locus_tag="EYO86_01555"
     CDS             24..248
                     /locus_tag="EYO86_01555"
                     /inference="COORDINATES: protein motif:HMM:PF00953.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41755.1"
                     /translation="MLISLQKRVVIINGSLFLFSYLPCQSFLDLLIINSINYLDGING
                     LSSGLFLFTLFNLFILLIWWVLGVVLCLFF"
     gene            212..>1154
                     /locus_tag="EYO86_01560"
     CDS             212..>1154
                     /locus_tag="EYO86_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIC41756.1"
                     /translation="MGAGCGALFILLILSLFDTTNSLRIIAFLGYIASLLVIFSIKRI
                     NWIWILVLLILPINFFLLPESWLRPIPLPYKALNKALNVPKANIITEYSSPLAQVTVV
                     ESPLVHFRHAPGLSLNAQSGPGEQLGIFIDADSFTPITKFNGDLESINYLDKQPDALP
                     FYLLEKPKILVLGMGGGSDVLRSFYFGAPVIDAVELNPQIVKIVTKDQKNYTNNFFSQ
                     SNIRIHTKEARSFIRGQKETWDIIQLSLFDSLTAANAGVFALKENNLYTVEAFRDYMG
                     HLNPGGFLTITRWLKLPPKDSLKLLATAVSALESDSKI"
BASE COUNT          354 a          178 c          204 g          418 t
ORIGIN      
        1 aaaaacggga tttctggaag tgtttgctga tttccttgca aaaaagggtg gtaatcataa
       61 atggctcact atttttattt tcctacttac cgtgtcaatc gtttctggat ttattaataa
      121 ttaattcaat aaattattta gatggtataa atggtctatc ttctggttta tttttattta
      181 ctctattcaa tctatttatt ttgctgattt ggtgggtgct gggtgtggtg ctttgtttat
      241 tcttttaatt ttgtccttat ttgataccac taattcttta aggattattg catttctcgg
      301 ttatattgct tcattgcttg taatattttc aattaaaaga ataaattgga tatggatact
      361 agtattatta attcttccga taaacttttt cttgttaccc gaaagttggc tgagacctat
      421 tcccttgcct tacaaagctt taaataaggc tcttaatgtt ccaaaggcaa atattattac
      481 tgaatattcc agtccattag cccaggttac tgtggtagaa agtccattag ttcactttcg
      541 acatgctcct ggattaagtc ttaatgccca gtcagggccg ggagagcaac taggtatttt
      601 tatagatgct gatagtttta caccaatcac aaaatttaat ggtgacttag aaagcattaa
      661 ttatttagat aaacaaccag atgcattacc attttatttg ttagaaaaac caaaaattct
      721 tgttttagga atgggtggtg gtagtgatgt tttgcgtagt ttttattttg gcgctccagt
      781 aatcgatgca gtggaactta atcctcaaat agtaaagatt gttactaaag accaaaaaaa
      841 ttacaccaac aatttttttt ctcaatcaaa cattagaatt catacaaaag aggcaagaag
      901 ttttattaga ggacagaaag aaacttggga cataatccag ctttcattgt ttgattcttt
      961 aactgctgca aatgctggag tcttcgctct taaggagaat aacttatata ctgtagaggc
     1021 atttagagac tatatgggtc atttaaaccc tggaggtttt ttaacaatta cgcgttggct
     1081 taaactacca ccaaaagata gcctaaaact tttggcaaca gcagtaagcg cgcttgaaag
     1141 tgatagtaaa ataa
//
LOCUS       DTVV01000130            1556 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1340469, whole genome shotgun sequence.
ACCESSION   DTVV01000130 DTVV01000000
VERSION     DTVV01000130.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1340469"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="EYO86_01565"
     CDS             <1..328
                     /locus_tag="EYO86_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIC41757.1"
                     /translation="IITIDEYYEMGFTEVIKIVKNTLGDTQAYLTFDIDGIDPTFAPG
                     TGTPEVGGFSVREAQLIIRELNTINFVGADVVEVSPPFDVNNMTSLVASTIAFEILCT
                     MTRSNL"
     gene            complement(348..1397)
                     /locus_tag="EYO86_01570"
     CDS             complement(348..1397)
                     /locus_tag="EYO86_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020027519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HIC41758.1"
                     /translation="MNYIKKKIGYIGSKEIYLISFTNDNNFTISFYNLGGYIHCVLIP
                     YKNNPNKCEDVILGYNKFEDCKISPSYFNSIIGRVGNRIANSLFYLNGKKYKLFNNEG
                     NHHLHGGENGFNKKVWNVNSIRKDKNELSCELEYFSKHLEEGYPGNLNCKTTYTLNND
                     NEFIIIFNASSDEDTVVNMTNHNYWNFHGHNDYYQNITNHSIKIYSHSVCENDSASIP
                     TGNLIGVKNTKFDFQNRKNISQEFLDEGGVDNNYDIGENLSIRKVAEVCSNLTKMGVT
                     YFTNQPGVQFYTGNMMANEYNGKENKKYGKNFGLCFEPQFFPDAINQPKFKSPILKAG
                     ENYLSSIVMRLGNDF"
BASE COUNT          528 a          240 c          219 g          569 t
ORIGIN      
        1 aattatcacc attgatgagt attatgaaat gggatttaca gaagtcataa aaattgtaaa
       61 aaatactttg ggtgatactc aggcatattt aacatttgat attgatggaa ttgatccgac
      121 ttttgcacca ggtactggaa ctcctgaagt gggcggattt agtgtaagag aagctcagtt
      181 aattattaga gagttgaata caattaattt tgttggcgct gatgttgttg aagtatcccc
      241 gccatttgat gtcaacaata tgacctccct tgtcgcgtca acaattgcat ttgaaatctt
      301 gtgcaccatg acacgaagca atttataaaa tgaaacaata gataagatta aaaatcatta
      361 cctagtcgca ttactatact tgaaagataa ttttctcctg ctttaaggat aggactttta
      421 aatttaggtt gatttattgc atcagggaaa aactgtggct caaaacataa accgaagttt
      481 ttgccatatt ttttattttc ttttccgtta tattcattag ccatcatatt acctgtataa
      541 aattgaacac ctggttgatt agtaaaatag gttacaccca tttttgttaa attactgcac
      601 acttctgcca ctttccttat tgataaattc tccccaatat cataattatt atcaactccg
      661 ccctcgtcca aaaattcctg gctaatattt tttctatttt ggaaatcaaa tttagtattt
      721 ttgacaccaa taagatttcc ggtgggaatt gatgcgctat cattctcaca aacactgtga
      781 ctataaattt taatcgaatg atttgttata ttttgataat aatcattatg tccatgaaaa
      841 ttccaataat tatgattggt catgttaaca actgtgtctt catcacttga agcattaaat
      901 attatgataa attcattatc gttattaagt gtgtaagtag ttttgcaatt aagattacca
      961 ggatagcctt cttctaagtg tttggaaaaa tattctaact cacaactcaa ttcatttttg
     1021 tcttttctga tactatttac attccaaact tttttattga aaccattttc tcctccatgc
     1081 aagtgatgat ttccttcatt attgaataat ttatattttt taccattaag ataaaataaa
     1141 gaattagcaa tacggtttcc tactcttcca ataatactat taaaataaga aggggaaatt
     1201 ttacaatctt caaacttgtt ataaccaagt attacatcct cacatttatt aggattattt
     1261 ttatatggaa ttaatacaca gtgaatatat ccacccaagt tataaaatga aatagtaaaa
     1321 ttattatcgt tggtaaaaga aataaggtat atttctttac tgccaatata gccaattttt
     1381 tttttaatat aattcataag cttgtaatac gatataaaaa atatttataa acatatcttt
     1441 agttgaaata aaaagatcat ctgctaatta attctaatct tacatgagtg aatttgatat
     1501 aatcatagtc ggtgcgggat cagcaggaag tatagcggct aatagacttt cacaag
//
LOCUS       DTVV01000131            1730 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1340876, whole genome shotgun sequence.
ACCESSION   DTVV01000131 DTVV01000000
VERSION     DTVV01000131.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1340876"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="EYO86_01575"
     CDS             <1..190
                     /locus_tag="EYO86_01575"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIC41759.1"
                     /translation="LGMSFLLVALQHADAGIVATLSSTSPVLVLFLIWVLTKKLPTLG
                     AWIGTVLAIVGTGLIFIY"
     gene            complement(173..>1730)
                     /gene="aspS"
                     /locus_tag="EYO86_01580"
     CDS             complement(173..>1730)
                     /gene="aspS"
                     /locus_tag="EYO86_01580"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIC41760.1"
                     /translation="TGKVLKRSSDTINTSLKTGEIEIVIKAFEILSKSDILPLPVNSD
                     IEYGEEVRLKYRYLDLRRNKLHKNILLRNKVISSIRTKMLKRGFIEFQTPILTSTSPE
                     GARDYLVPSRIHQGKFYALPQAPQQFKQLVMIAGFDKYFQIAPCFRDEDSRSDRSPGE
                     FYQLDFEMSFVTQEDVFEAIEPVLFEIFDENKSFSRDKSAQVGKYPFPKIPYHESLEK
                     YGTDKPDLRNPLLIKNCTEVFVDSNFKIFANKVKKGSVVKGIQITNTEDKPRSFFDKL
                     NDWAKEEGEAGLGYITYQQGEYKGPIAKHLSKDKLETLKLNEGDSIFFVCNSIKEAQL
                     FSGKVRDKLCMELNLLDKNSFQFCWIVDFPMYEIDEKTNKIQFSHNPFSMPQGEMESL
                     KKMDPLEVKAYQYDIVCNGVELSSGAIRNHKPEIMYKSFEIAGYNKETVEKNFSGMLN
                     ALKFGAPPHGGSAPGIDRMVMLLADEPNIREVIAFPMNQQAMDLMMNAPSEIEKDRLE
                     ELGLKLIDKD"
BASE COUNT          535 a          308 c          247 g          640 t
ORIGIN      
        1 attgggaatg agctttttac tggttgctct tcagcatgca gatgccggta tagtagctac
       61 actttcctct acaagtcccg ttttggtttt atttttaatt tgggttttaa ctaaaaaatt
      121 accgacattg ggagcttgga taggaactgt attagctatt gtaggaactg gattaatctt
      181 tatctattag ttttagaccc aactcctcaa gtctatcttt ttcaatttca gatggggcgt
      241 tcatcattaa atccatagcc tgctgattca ttggaaaagc aatgacttct ctaatattag
      301 gttcatcagc taataacatc accattctat ctatgcccgg agcacttccg ccatgaggtg
      361 gggcaccaaa tttcagtgcg ttaagcatgc cagaaaaatt tttttctact gtttccttat
      421 tatatccagc aatttcaaaa gacttataca taatttcagg tttgtgattt ctaatagctc
      481 ctgatgacaa ttcaactccg ttacagacta tatcatattg atatgcttta acttccaaag
      541 gatccatttt ctttaatgat tccatttctc cttgtggcat ggaaaaagga ttatgactaa
      601 attgaatttt gtttgttttt tcatcaatct catacattgg aaaatcaaca atccaacaga
      661 attgaaatga gtttttatct aataaattta attccataca aagtttatct ctaactttac
      721 cagaaaataa ttgcgcttct ttaatactgt tacatacaaa aaatatggaa tctccttcat
      781 taagctttaa agtttcaagc ttatctttcg ataaatgttt tgctataggt cctttatatt
      841 cgccttgttg gtatgtaata taccccaatc cagcctcacc ttcttcttta gcccaatcat
      901 ttagtttatc aaaaaaactt ctaggtttgt cttctgtatt tgtaatttga atccccttaa
      961 ctacagatcc ttttttaact ttatttgcaa atatcttaaa atttgaatca acaaaaactt
     1021 cagtacaatt tttaattaat aagggattcc gcaaatcagg tttatctgtt ccatattttt
     1081 ctaaagattc atgatatgga attttaggga aaggatattt acctacttgg gcggatttat
     1141 ctctactgaa tgatttgttt tcatcaaata tttcaaataa aacaggctct attgcctcaa
     1201 atacatcttc ctgggtaaca aaactcatct caaaatctaa ttgataaaac tcacctggag
     1261 aacgatccga cctgctatcc tcatctctaa aacaaggagc tatttgaaaa tatttgtcaa
     1321 atccagcaat catcaccaat tgcttaaatt gttgtggagc ttgtggtaat gcataaaatt
     1381 taccttgatg aattctcgat ggcaccaaat aatctcttgc tccctcgggg gaagtagaag
     1441 ttaatatagg agtttgaaat tctataaatc ctcttttaag cattttagtg cgaattgaag
     1501 aaataacttt atttcgaagt aaaatatttt tatgaagttt attgcgtcta agatctaaat
     1561 atctatattt taatcttact tcttcaccat attcaatgtc agaattaact ggtaaaggta
     1621 aaatgtctga ttttgataaa atttcaaaag cttttattac tatttcaatc tcccctgttt
     1681 taagagatgt attaattgtg tcagaagatc tttttagaac cttacctgta
//
LOCUS       DTVV01000132            1537 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1343540, whole genome shotgun sequence.
ACCESSION   DTVV01000132 DTVV01000000
VERSION     DTVV01000132.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1343540"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1..1537
                     /locus_tag="EYO86_01585"
                     /pseudo
     CDS             1..1537
                     /locus_tag="EYO86_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544330.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type II/IV secretion system protein"
BASE COUNT          568 a          236 c          293 g          440 t
ORIGIN      
        1 aaaatctaaa ctggaaaccg cattcgagct taatctgact gatgaaaaaa aaataataac
       61 aattttgtca aaggtctatt ctttagatac cgtcgactta aaaaaatata aaattaacga
      121 taaacttaaa aaagttgtac ctttagacta catcagaagt aacactttag ttccctttga
      181 gattgctaat ggaattttaa aaattgccat accagatgct tcaaaactca gtttaatgaa
      241 aaatcttaaa actatgacaa agatggatcc tgaactttat gcagctacaa tttcggatat
      301 tggtggtttt attacacgaa tgtcaggtgg agaagacaaa caaagttcaa gacaaaaatt
      361 agaacaggtt aaaaagaagg aaaaaattat cccgatcgaa gttgaatcag atgtaattaa
      421 ttttggtgat aaagttattg gggaagccat cgatatggga gctagcgata tacatattga
      481 ggcttttcga aatacgtcag cagtaagatt tagagttgat ggcatcttaa agattatgga
      541 taagttcaca aagtttttac acaataatta taatgctgtg gtcacaagag ttaaaattat
      601 atcaaaatta gatattgctg aacgaagaat gccacaagat ggagcttcga cttttaaaag
      661 aggcgacaag gaagtggata tgaggatttc cattttgcca acaaaaaata acgaacgtat
      721 tgtaatgcgt attttaaata aagatgctgg cgataaagca ttaaatgaac tgggatttga
      781 aacaaacgat ctcaaaaaac ttaccaaggc cgttacatct cctcaaggta tggttttagt
      841 taccggacca acagggagcg gaaaaactac tacactgtac agtattttaa aacatattaa
      901 caaaccttcc atgaacattt taactgctga agatccagta gaatatgagc ttgaaggaat
      961 aggtcaggtg catgtaagag aagatatcgg ttttactttt gcttcggctc tgcgatcttt
     1021 tttacgtcaa gatccacagg ttatattagt tggtgagatt cgagataaag atacagtaga
     1081 tattgcttta aaagcagcac taactggaca ccttgttttt agtactattc acacaaatga
     1141 tgcaccaagt actattacgc gtttacagaa tatgggcacg cccaattatt taatttcagc
     1201 agcgttgtca ttaattatgg cccaacgttt agctagaaaa atatgttccg aatgtaaaat
     1261 tatagatgaa aacgcaacac caaaattatt aaattcaata ggttttttac ccgaacaatc
     1321 cgcccgtgca aaaatttata aaggcaaagg atgcggtcat tgtaataaaa ctggctataa
     1381 aggcagaatg ggaatttatg agatcttgga ggttaccaag gaactcaaac atggtatttt
     1441 atccaattta ggacaaaacg agttggaagc tcttgctaaa aaaggtggat ttagaactat
     1501 aacagcatgg gccatgatct tttattgagc ggagatc
//
LOCUS       DTVV01000133            1302 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1345096, whole genome shotgun sequence.
ACCESSION   DTVV01000133 DTVV01000000
VERSION     DTVV01000133.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1345096"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="EYO86_01590"
     CDS             <1..406
                     /locus_tag="EYO86_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIC41761.1"
                     /translation="ISKVIDGRFPDYSKVIPKNNDKILQIKLDEFRNSIERVTTVSSD
                     RTKGLKMTISKDGVQLSVNNPNSGEGIENINAKFNSNDLNISFNSRYLTDIASQIENE
                     FIVINLKDANSPVLINDFSDKNSFYVVMPMKI"
     gene            554..>1302
                     /locus_tag="EYO86_01595"
     CDS             554..>1302
                     /locus_tag="EYO86_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIC41762.1"
                     /translation="MIKKKQQNNSKYSAESIKVLKGLEAVRKRPGMYIGDTDDGTGLH
                     HMVYEVVDNSIDEALAGYCKNIKVTINQDQSVSVEDDGRGIPVDIHKGEKKSAAEVIM
                     TQLHAGGKFDHDSYKISGGLHGVGVSVVNALSEKLSLKIFRDKKEYFVEFKDGKALSP
                     LKVKGKINKTGTLINFLPSREVFSDTKFSSSILQKRMRELAFLNKGLCIILVDKTGKK
                     EKEYKNKYEGGIQEFVEFLDKNKKTLVNKNDL"
BASE COUNT          514 a          154 c          225 g          409 t
ORIGIN      
        1 aatttctaaa gttattgacg gaagatttcc tgattacagt aaagttatcc caaaaaataa
       61 tgataagatt ttacagataa aattagatga atttagaaat tctattgaga gagtcactac
      121 cgtttcttct gatcgtacaa aaggtttaaa aatgactata tcaaaagatg gtgttcaatt
      181 atcagttaat aatccaaaca gcggcgaagg tattgaaaat attaatgcaa agtttaattc
      241 taatgatttg aatataagct ttaacagcag atatttgaca gatattgctt ctcaaattga
      301 aaatgaattt attgttatta atttgaaaga tgcgaactca cccgtgttga ttaatgattt
      361 ttcagataaa aatagttttt acgttgttat gccaatgaaa atatgattta ttttgcaaaa
      421 agacgatatt agattattat tatttttttc attaagtaac catttaatac caatgaaaat
      481 ttaagcagca gatttgccgc agatttaatt agattagaaa aaatgttaat atgctaatta
      541 agaattcatt tatatgatca agaaaaaaca acaaaataac tcaaaatata gcgctgaatc
      601 cataaaagta cttaaaggtt tagaagcagt tcgaaaaaga cctggaatgt atattggtga
      661 taccgatgat ggaacaggcc ttcaccatat ggtttacgag gtagttgata attctataga
      721 tgaggcttta gcaggctatt gcaaaaatat taaagttacg atcaatcaag accaatctgt
      781 ttccgtcgaa gacgatggaa gaggaatacc agttgatata cacaaaggag aaaagaagtc
      841 ggcagctgaa gttattatga cacagcttca tgctggtggt aaattcgatc atgattcgta
      901 caagatttcc ggaggacttc atggtgttgg cgtttcggta gttaatgctt tatcagaaaa
      961 attaagttta aaaatattca gagataaaaa agaatatttt gttgagttta aagatggaaa
     1021 agcgttatct cccttaaaag ttaaaggaaa aataaataaa acagggactc ttataaattt
     1081 cttaccatca agagaagtat tttctgatac taaattcagc tcatcaattt tgcaaaaaag
     1141 aatgagagaa cttgcttttt taaacaaagg tttatgtatt attttagtag acaaaacagg
     1201 aaaaaaggaa aaggaatata aaaacaaata cgaaggtggt atccaagaat ttgttgagtt
     1261 tttagataaa aataaaaaaa ctttggttaa taaaaacgat ct
//
LOCUS       DTVV01000134            1727 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1348220, whole genome shotgun sequence.
ACCESSION   DTVV01000134 DTVV01000000
VERSION     DTVV01000134.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1348220"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            7..762
                     /locus_tag="EYO86_01600"
     CDS             7..762
                     /locus_tag="EYO86_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC41763.1"
                     /translation="MSSIIEVQDLSKYYGKHLVYEKLNFDVKEGEFLSIIGPSGCGKT
                     TLLKILGGLIEHSSGNISINGQPVKNALKARKLGFVFQNPVLLPWRISSSNIELPLEI
                     QGINNSPKLSDEMLKIVGLEGFENYYPGELSGGMQQRVAIARALAFKPLILMMDEPFG
                     ALDEITRNHMNLELLRIWKEEKASISTVIFVTHSIPEAVFLSDRILVLSAGPAKIEEI
                     VDIDLPRPRKTEMKHSKRYLEFMEHLRKIIGED"
     gene            793..1557
                     /locus_tag="EYO86_01605"
     CDS             793..1557
                     /locus_tag="EYO86_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC41764.1"
                     /translation="MKKGSGTIVIPILFSVVIFTVWELLVFLLEIPEYLLPPPSTIFN
                     ELGTNFSILLGHMAMTMLAAVSGYLLANGIGFCAGVIFAHSKTIEKGIYPYAIALKTT
                     PVIAMAPLLVLWFGTDLESKIATAALICFFPIIVNSVKGLTSVDRDSLDLFKSYSASK
                     WQIFIKLRLRTSLPYVFSALKISTGLSVVGAVVGEFVGANVGIGYLILVSSYHLETAT
                     MFAAIMMSALGGVTFFTIVSLIEKKVIFWQRALETS"
BASE COUNT          571 a          262 c          331 g          563 t
ORIGIN      
        1 aactacgtgt catcaataat agaagttcag gatttatcaa aatattacgg aaaacacctt
       61 gtttatgaaa aattaaattt tgatgtaaag gaaggagaat ttctttctat aattggtccg
      121 agtggctgtg ggaaaactac tctgctcaag attctaggtg gtttgattga acactcaagt
      181 ggtaatatta gtataaatgg acagcctgta aaaaatgctt taaaggcaag gaaacttggt
      241 tttgtttttc aaaatccagt cttattgcct tggcgaatct cctctagtaa tattgaactt
      301 ccattagaaa ttcaagggat taacaattct ccaaagttat ctgacgagat gttgaaaatt
      361 gttggtcttg aaggttttga aaattattat ccgggagaat taagtggggg aatgcaacaa
      421 agggtggcta tagccagagc gcttgcattt aaaccactaa ttttaatgat ggatgaacca
      481 tttggagcgt tggacgagat aacaagaaat catatgaatc tagaattatt aagaatatgg
      541 aaagaagaaa aggcctcaat atccactgta atttttgtaa ctcattcaat tcctgaagct
      601 gtatttttat cagacaggat tctcgttttg tctgctggac cagcgaaaat tgaggagatt
      661 gtagatatag atttaccgag accaagaaaa actgagatga agcattctaa aaggtacctc
      721 gagtttatgg aacatcttag aaaaataata ggtgaggact aggagtaatt ggtattacag
      781 ccttttaaga aaataaaaaa aggatccgga accattgtaa ttccaatctt attttctgta
      841 gtaatcttca ccgtttggga acttctagtt ttcttactag agatcccaga atatctcttg
      901 cctccaccgt ccacgatctt taatgagctt ggaacgaatt ttagtattct tttaggacat
      961 atggcaatga ctatgcttgc tgcagtatca ggatacttat tagccaacgg catagggttt
     1021 tgtgctggtg taatattcgc acattcaaaa actatagaaa aaggcatcta tccatatgca
     1081 attgcactta aaacaacacc agtaatagca atggcgccac ttttagtttt atggtttgga
     1141 acagatttag aatctaaaat cgcaactgct gctcttattt gcttctttcc tatcattgta
     1201 aattctgtta aaggattaac atccgtagat agagattctc tggatttatt caagtcatac
     1261 tctgcaagca aatggcagat atttataaaa ttaagactgc gaacttcgct tccctatgtt
     1321 ttttctgccc taaaaatttc aacagggtta agcgttgttg gtgcagtagt tggagaattt
     1381 gttggagcaa atgtaggaat tggatatctt attctggttt catcatatca tttagaaact
     1441 gctactatgt tcgcagccat tatgatgagt gctttaggag gtgttacatt ttttacaatt
     1501 gtcagcctta tagaaaaaaa ggtaatattt tggcagaggg cattagaaac tagctaaaat
     1561 aataaatctg aaaaatctag tttaccgtta aaacatttgg aacatgttac tcacagaaaa
     1621 aagcttaaga atcatggtaa ttggtagcga tcagaaaatt atttttattc tgtttttaat
     1681 tatcagcagt taacataaag aaaatctatt cagtttttta gatcatg
//
LOCUS       DTVV01000135            1041 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1348769, whole genome shotgun sequence.
ACCESSION   DTVV01000135 DTVV01000000
VERSION     DTVV01000135.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1348769"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="EYO86_01610"
     CDS             complement(<1..220)
                     /locus_tag="EYO86_01610"
                     /inference="COORDINATES: protein motif:HMM:PF07364.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcystin degradation protein MlrC"
                     /protein_id="HIC41765.1"
                     /translation="MKNILFIGIFHETNCFIKEKTKLKDFEILKKKEILDLKNDFSQI
                     SGFLNFCKTKNLNVFSTVNFTATPSGIVE"
     gene            276..935
                     /locus_tag="EYO86_01615"
     CDS             276..935
                     /locus_tag="EYO86_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIC41766.1"
                     /translation="MKKDIIEKCKNNLYSAVIADTLDSFGYHKQAVYTTINSLDSELV
                     LCGFARTGTYMPIYHDDENTNVYEHELALIDSLLEGEVCVLSCNNNRNIAPWGELLTT
                     RAKYLKAAGCLIDGCIRDSKAIKKMKFPIFSNGTNPVDTKFRGKMIFSDVPAEVGGVL
                     INTGDLIFGDQDGVVSIPSKLINQVVEKSFQKVSSENTVRKELNEGQSLKEVFIKHGI
                     L"
     gene            complement(965..1041)
                     /locus_tag="EYO86_01620"
     tRNA            complement(965..1041)
                     /locus_tag="EYO86_01620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1005..1007),aa:Arg,seq:cct)
BASE COUNT          382 a          143 c          163 g          353 t
ORIGIN      
        1 cttcaacaat tccagatgga gtagccgtaa aatttacagt tgaaaaaaca ttcaaatttt
       61 tggttttaca aaaatttaaa aaacctgata tttgagaaaa atcgtttttt aaatctaata
      121 tttctttttt ctttaaaatt tcaaaatctt taagtttagt tttttctttt ataaaacaat
      181 tggtttcatg aaaaatacca atgaataata tatttttcat aatatttcct aaaatatata
      241 tttatttcaa tctaataaga tagtaatata aattaatgaa aaaagatatt atagaaaaat
      301 gcaaaaacaa tctttattct gcggtaattg ctgatacttt agattctttt ggttatcata
      361 agcaagctgt ttatactaca attaactctt tagattcaga gcttgtactt tgtggatttg
      421 ctagaacagg aacttacatg ccaatttatc atgacgatga aaatacaaat gtatacgaac
      481 atgaacttgc attaatcgat agtttgttag aaggagaagt ttgtgtttta tcttgtaaca
      541 ataatagaaa tatagctcca tggggagagc ttttaacaac aagagctaaa tatctaaaag
      601 cagcaggatg tctaattgac ggatgtataa gagactccaa agcaataaaa aaaatgaaat
      661 ttccaatttt ttcaaatggc acaaatcctg ttgacacaaa atttagaggg aaaatgatat
      721 tttctgatgt tcccgcggaa gtagggggtg ttctaataaa tacaggagat ttaatttttg
      781 gtgatcaaga tggagtggta tcaataccat ctaaattaat taatcaagta gttgaaaaat
      841 ctttccaaaa agtttcgtca gagaatactg ttcgtaaaga gctcaatgaa ggtcaatcac
      901 taaaagaagt attcattaaa catggtatac tttaattttt aataactata ttctttaagt
      961 tttctggcgt gcccggcgag attcgaactc acgaccccca gattaggaat ctggtgctct
     1021 atcctactga gctacgggca c
//
LOCUS       DTVV01000136            1095 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1348859, whole genome shotgun sequence.
ACCESSION   DTVV01000136 DTVV01000000
VERSION     DTVV01000136.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1348859"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1095
                     /locus_tag="EYO86_01625"
     CDS             <1..>1095
                     /locus_tag="EYO86_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIC41767.1"
                     /translation="KKLGIADYSKLSEEAKIKFLSKEYKSKRPLIPQNIKLDADDNET
                     WTTFKMISESPRECLGAYVISMASNVSDILAVMLLQKEAGVKSCLRIVPLFETLSDLQ
                     NSHIVMENLFKHSWYVNYFKKNQEIMIGYSDSSKDAGKFAASWAQYCAQEKLGNIATK
                     YKIKLTLFHGRGGSVGRGGGPVYAALLSQPPGTVNARTRVTEQGEVIQQKYGSESLAE
                     YSLGTYIGAVLEATLSPPIQPKKEWRNLIDEMSILSSKSYRRYLNNDKNFLRYFDEIT
                     PKNIIGKLYIGSRPAKRKQSQDIKNLRAIPWVFAWTQIRLVLPAWLGTTEALQLASRG
                     KNKLILKDMLYRWPFFYEMMDMLDMVLTKT"
BASE COUNT          389 a          169 c          205 g          332 t
ORIGIN      
        1 taaaaaactt ggaatagctg attattctaa actttcagaa gaagcaaaaa ttaaattttt
       61 aagtaaagaa tataaatcta aacgtcccct tataccccaa aacattaaat tagatgctga
      121 tgacaatgaa acttggacaa cttttaaaat gatatcagag tcacccagag aatgtctcgg
      181 agcctatgtt atttccatgg catcaaacgt ttcggatatt ttagctgtaa tgcttttaca
      241 aaaagaagca ggagttaaat catgtttgcg catagttcct ttatttgaaa ctttatctga
      301 tcttcaaaat tcccatattg taatggaaaa tttgtttaaa cattcttggt acgtgaatta
      361 tttcaaaaaa aatcaagaaa taatgattgg atattcagat tctagtaaag atgccggaaa
      421 atttgcagca agttgggcgc aatattgtgc acaagaaaaa ttaggcaaca ttgcaacaaa
      481 atataaaatt aaactaactt tgtttcatgg cagaggaggt tcagtaggta gggggggagg
      541 accagtttat gcagccttac tctctcaacc gcctggaact gttaatgcaa gaacgcgagt
      601 aacagagcag ggagaggtga tacaacaaaa atatggctct gagtccttag ctgaatatag
      661 tttaggaaca tatattggag ctgtattaga ggctactctt tcacctccta ttcagcccaa
      721 aaaggaatgg cgcaatttaa tagatgaaat gagcatccta tcaagtaaat cctacagaag
      781 atacctaaac aatgataaaa attttttacg atattttgat gagataacac caaaaaatat
      841 tattggtaag ctatatattg gttcaagacc agctaagcgt aagcaatctc aagatattaa
      901 aaatttaaga gccattcctt gggtttttgc ctggacacaa atacgtttgg ttttacccgc
      961 ttggcttggt actacagagg cattgcaatt ggcttcgaga ggtaagaata aattaatttt
     1021 aaaggatatg ctttatcgtt ggcctttttt ttatgaaatg atggatatgc ttgatatggt
     1081 tttaactaaa acaga
//
LOCUS       DTVV01000137            1214 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1348927, whole genome shotgun sequence.
ACCESSION   DTVV01000137 DTVV01000000
VERSION     DTVV01000137.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1348927"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            403..594
                     /gene="rplK"
                     /locus_tag="EYO86_01630"
                     /pseudo
     CDS             403..594
                     /gene="rplK"
                     /locus_tag="EYO86_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953369.1"
                     /note="binds directly to 23S ribosomal RNA; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
     gene            594..>1214
                     /gene="rplA"
                     /locus_tag="EYO86_01635"
     CDS             594..>1214
                     /gene="rplA"
                     /locus_tag="EYO86_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIC41768.1"
                     /translation="MSSKRYNKLPKGTKSITSESIDKLISTIKKNCTTKFDESIDLNL
                     QIKNKQKKSEINLRTVVNLPSGTGKKIKVAVVCEDTNMSEAKGAGADIVGGDDFIEKM
                     KEGNLDFEKLICTPGMMVKLSKIGKVLGPKGLMPNPKLGSVTNDLKKAITDAKSGQVE
                     IKNDKDGNIGLSIGKKSFSDDKLVQNFKAVFEILEREKANSIVKGDL"
BASE COUNT          499 a          154 c          210 g          351 t
ORIGIN      
        1 taaaatgttg taattgattt gaatcttctg aagttggcta tcagtataca ttatagaagt
       61 atttttgtgt gcatactaat ctatggcatc ccctagcgac gcaggtttat tggtgaatta
      121 tttaatactt taatcatctt gtaactcaat atatagatca tttctagggt ttttcttatt
      181 aatatcttga caaatccttt aaaaactact actaaaaact actactaata agaatgtttt
      241 caaggattta ccatcatacc taagtttaca gatcatgaaa accttgtcaa gcagtctgga
      301 gctaagtcta agaactggta tataagaatt caattgccta aagatgttag acatagattt
      361 gataacaaaa aacaattctg gaaaagcgct aagacttcta atctaaaaga agcagctaaa
      421 ttaaaaagtg gatctaatga acctggaata caaatagtcg cttcaattac aaaaaaacaa
      481 gctgaagaaa tagctaaaaa aaaaatggca gacttaaaca ctaatgactt agagcaagca
      541 gttaaaatta tatcaggctc agctagatca atgggtatag aggtaaaaga ataatgtctt
      601 caaaaaggta taacaaatta ccaaaaggta cgaaatctat tacatcagag tcgattgata
      661 aattgatatc tactatcaaa aaaaattgta caactaaatt tgacgagtca atagatttaa
      721 atttacagat taaaaataaa caaaaaaaaa gtgaaataaa cttgagaaca gttgttaatt
      781 tgccttcagg tacaggtaaa aaaattaaag tagctgtagt gtgcgaagat acaaatatga
      841 gtgaagctaa aggagcaggt gcagatatag ttggtggaga tgattttata gaaaaaatga
      901 aggaaggaaa tttagatttc gaaaaactta tatgtacacc aggaatgatg gttaaactat
      961 ctaaaattgg taaggtttta ggcccaaaag gtttaatgcc taatccaaaa cttggctcag
     1021 ttactaacga tcttaaaaaa gcaattactg atgcaaaatc tgggcaagtt gaaattaaaa
     1081 atgacaaaga cggaaacata ggattaagta taggcaaaaa gagtttttca gatgataagt
     1141 tagttcaaaa ttttaaagca gtttttgaaa ttcttgaaag agagaaagcc aacagtatag
     1201 ttaaaggtga ttta
//
LOCUS       DTVV01000138            2389 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1351109, whole genome shotgun sequence.
ACCESSION   DTVV01000138 DTVV01000000
VERSION     DTVV01000138.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1351109"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            507..2318
                     /locus_tag="EYO86_01640"
     CDS             507..2318
                     /locus_tag="EYO86_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIC41769.1"
                     /translation="MVNRFSVPNLYDCTKHLANVASRKIQPELILKNAHILSTYTNRI
                     LENKEIWISKGRIACIEENGQAEKIFNYKNLVLFDAQNNILAPGLIDPHMHIESSMMT
                     GCAYAEAALLNGTTTIFCDSHEIGNVCDTEGIEWMLEDCRQAPLSIFLTLPSTIPATN
                     NILETAGGDLTPQKISKLFDQWPEILGLGEKMDFVSVNNGNERSHDIITETLKRNLPI
                     SGHVFGREFVAAYAASGITDTHEAEEKYFTNDLLEAGLWIFLRGGNPETPWNSLPESL
                     KAITEFGANSKRVCVCTDDRDADDLFNFGLDWVVRQTHALGIINKAESWSLGSLHPAT
                     RYNIDRDYGALGHSRRADIVMLDDNMEVQNTWLGGQLVVEKKKITSLLDDQLSKQRYR
                     YPAQAYNTINIVNDINYLPKIPKKKKFKINVIRTELPGIMTFRETVFIENKINSWSEI
                     LSLHDLCHICVIERHGKTGEYAHGFIKKFNLKEGAVASSVGHDAHNLIVAGMNKEDMK
                     FAIDLIKEFKGGVVIVNNGKLISSIKLPVAGLLSDKRASEVAKENKKFKKNWNKAGCT
                     LAYMGFNLLPLSVIPNLRITNKGLVDVNKMKLIPLFE"
BASE COUNT          904 a          301 c          421 g          763 t
ORIGIN      
        1 cataaaaaag aattagaatt atggaatagt attaattttt caccactttc ttcttcttgg
       61 tatcaagaag taggacaagg tatgggcatt acattattaa tggccaatga aaaattagct
      121 tatactttaa gtgatcgagg aacttggatt gcctttaata aaaaagaaaa tttaagtatc
      181 atttgtgaaa atctgccccc tttgttcaac caatatggtt tgattattgt agatccttta
      241 gtaaatagta atctagatat tgaaaatgct aatatttatg taaattggct tatttctgat
      301 agaggaaaag agttaattaa taattttaga aaaaaaggac aacaattatt ttattttaat
      361 caccattaaa aattcaacaa atactaatta tatataaaca ttataatttg cattattaaa
      421 attacaatat ttctaaaaat attgattgag acacagagta gatgataata tgatttatta
      481 agaaataatt taaatcaaaa tactttatgg ttaatagatt ttcagttcct aatttatatg
      541 attgcacaaa acatttagct aatgttgcat ctcgcaaaat tcaaccagaa ttaattctta
      601 aaaatgcgca tatattatca acttacacaa atagaattct tgaaaataaa gaaatttgga
      661 tatcaaaagg gagaattgca tgtattgaag agaatggtca agctgaaaaa atttttaatt
      721 ataaaaattt ggttttattt gatgcacaaa ataatatcct ggcaccagga cttattgatc
      781 cgcatatgca tattgaaagc agcatgatga ctggttgcgc ttatgcagaa gctgctttat
      841 taaatggtac cacaacaata ttttgtgatt cacatgaaat aggaaatgtt tgcgataccg
      901 aaggcataga atggatgctt gaggattgta ggcaagcacc tttatcaatt tttcttacac
      961 ttcctagcac tataccagct actaataaca ttttggaaac tgctggagga gatttaacac
     1021 ctcagaaaat ttcaaaatta tttgatcagt ggccagaaat acttggtctt ggagaaaaaa
     1081 tggactttgt ttctgtaaat aacggaaatg aaagatctca tgacatcata acagaaactt
     1141 taaaacggaa cttgccaatt agtggacatg tatttggaag agaatttgta gctgcttatg
     1201 cagcaagtgg aataactgac actcatgaag cagaagaaaa atactttaca aatgatctgc
     1261 ttgaagcagg tttatggatt tttttgagag gaggaaatcc tgaaactcct tggaatagcc
     1321 tccctgaatc tttgaaagca attactgaat ttggcgctaa ttcgaaacgt gtttgtgtat
     1381 gcactgatga cagagatgca gatgatttat ttaattttgg tttagattgg gttgtaagac
     1441 aaacccatgc attaggtata ataaataaag cagaatcttg gtcattagga tcactgcatc
     1501 ctgccacaag atataatata gatagggatt atggagcttt aggacatagc agaagggctg
     1561 atatagtgat gctggacgat aatatggaag ttcaaaatac ttggttggga gggcagttag
     1621 ttgttgaaaa aaagaaaatt acatcacttc tggatgacca attatcaaaa caacgatatc
     1681 gatacccagc tcaagcatat aatacaatta acatagtgaa tgatattaat tatttaccta
     1741 aaatacctaa gaaaaaaaaa ttcaaaataa atgtaataag aaccgaatta cctggcatca
     1801 tgacttttag ggaaacagtt ttcatagaaa ataaaataaa ttcatggagt gaaattttaa
     1861 gcttacatga tttatgtcat atatgtgtaa tcgaacgtca tgggaagaca ggtgaatatg
     1921 ctcatggttt tattaaaaaa tttaatttaa aagaaggtgc ggttgcaagt agtgttggcc
     1981 atgatgccca caatttaatt gttgctggta tgaacaaaga ggatatgaaa tttgctattg
     2041 atttaattaa agaatttaaa ggaggagtag taattgtaaa taatggtaaa ttaatttcta
     2101 gcataaaact tcctgttgca ggattgcttt cagataaaag agctagtgaa gtagcaaagg
     2161 aaaataaaaa atttaagaaa aattggaata aagctggatg tacacttgct tatatgggct
     2221 ttaatttact gcctttatcc gtaattccta atttacgaat tacaaataaa gggttagtag
     2281 atgtaaataa aatgaagtta atccctttat ttgaataaaa aaatgcccaa agtttttaaa
     2341 atttgcattt catctaaatt tgaccaggat atggaagata tttatacag
//
LOCUS       DTVV01000139            1304 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1351153, whole genome shotgun sequence.
ACCESSION   DTVV01000139 DTVV01000000
VERSION     DTVV01000139.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1351153"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="EYO86_01645"
     CDS             complement(<1..198)
                     /locus_tag="EYO86_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41770.1"
                     /translation="MYKIAIIRESRSDDRRTPLVPAHIKELLSTFSDLSISVQPSEHR
                     CFSDQEYEEQGAIITEDLSACN"
     gene            complement(200..>1304)
                     /locus_tag="EYO86_01650"
     CDS             complement(200..>1304)
                     /locus_tag="EYO86_01650"
                     /inference="COORDINATES: protein motif:HMM:PF16653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HIC41771.1"
                     /translation="GSGLSSIPGIRRLASNNYNLTVWNRTLSKAQKSINHVKTDKVSA
                     KTFNINEIKGTVEQGDIIVSQLTASMHMDIAKICLEKKCHFVTTSYLAPDIKKLNEQA
                     VAQNLTFINEVGLDPGIDHFFSHLLVQELKSLNLNNLSVSYKSYCGGIPAVPNDFKYK
                     FSWSPVGVIRALNSPAKFTQNFEEKLVPQPYKEVKNHLINSESFEAYPNRDSLPYIEE
                     YLFSPEWKIKEFVRGTLRLNGWTLAWREIFNMLEKQDANIEEQIQQKSNELWKNYRYD
                     KNEEDRVVLWVNLEAEQQDKKVFSSTYQLDEKGSGENTAMAKLVSITLSAAVDLMLQN
                     KIQPGVQAAPSNKHIIDYFFKVLSDYSIKIDHH"
BASE COUNT          382 a          222 c          229 g          471 t
ORIGIN      
        1 gttacaggca ctcaaatcct cagtgatgat tgcaccttgt tcttcgtatt cttgatctga
       61 aaaacagcga tgctcggaag gttggacgga aatacttaga tcagaaaatg tacttaatag
      121 ttctttgata tgtgctggca ctaaaggggt tcgcctgtca tcagatcgag attctctgat
      181 tatagcgatt ttatacatat taatgatgat ctattttaat tgagtaatca gataatactt
      241 tgaaaaaata atcaataata tgtttatttg acggtgcagc ttgaacccct ggctgaattt
      301 tattttgtaa cattaaatcc acagcggcac ttagtgtgat agaaaccaat tttgccattg
      361 ccgtattttc acctgatcct ttttcatcaa gctggtaagt tgatgaaaat acttttttgt
      421 cttgttgttc agcttccaaa ttaacccata aaacaactcg gtcttcttca tttttatcgt
      481 atcgatagtt cttccaaagt tcattgcttt tttgttgaat ttgctcttca atgtttgcat
      541 cttgtttttc aagcatgttg aaaatttccc tccaagctaa agtccaacca ttcaatcgaa
      601 gtgtacccct tacaaattct ttgattttcc actctggaga aaataaatat tcttcaatat
      661 aaggtagaga atcgcgattg ggataagcct caaagctttc actgttaatt aagtgatttt
      721 tgacttcttt gtagggttga ggaacaagtt tttcctcaaa gttttgagta aatttagctg
      781 gactgtttaa agctctgatt actccaactg gagaccaact aaacttatat ttaaaatcgt
      841 taggtacagc cggtatcccc ccgcagtaag atttataact cacagataaa ttattgagat
      901 taagtgattt aagttcttgg accagtaagt gtgaaaaaaa atgatcaatg cctggatcta
      961 gtcctacttc atttataaat gtaagatttt gagcaacagc ttgttcattt agctttttaa
     1021 tatcaggagc taaataactt gttgttacaa aatgacattt tttctcaaga caaatttttg
     1081 caatatccat atgcatgctt gcggtaagtt gggatactat gatgtcccct tgttcaactg
     1141 ttccttttat ttcatttatg ttaaaagttt ttgcggaaac tttgtcagtt ttaacatgat
     1201 taatgctttt ttgggctttt gagagagttc tgttccagac agttaagtta taattatttg
     1261 atgctaagcg cctgatacca gggatagagg aaaggcctga acca
//
LOCUS       DTVV01000140            1019 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1351495, whole genome shotgun sequence.
ACCESSION   DTVV01000140 DTVV01000000
VERSION     DTVV01000140.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1351495"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            170..328
                     /locus_tag="EYO86_01655"
     CDS             170..328
                     /locus_tag="EYO86_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010170949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41772.1"
                     /translation="MSLVIAKHISDNIMVLYFGKLVEMNSSKELFSNPLHPYTKKIIS
                     SVPTFTSL"
     gene            459..>1019
                     /locus_tag="EYO86_01660"
     CDS             459..>1019
                     /locus_tag="EYO86_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC41773.1"
                     /translation="MLKFIKATCLTLLFVGFISPVYSATIKWSMPGDSLTLDPHAQNE
                     GPTHMVSRQVYESLVTRGLDMSIEPQLATSWEATDPETWVFTLRDGVVFSDGKALRAS
                     DVEFSINRALSGASDMIELIESITSVKALSPSTVEIKTEGPNPILLNQLSQIFIMSEG
                     WSKKNGCELPQDFNAEEATHCSSNAMG"
BASE COUNT          341 a          154 c          168 g          356 t
ORIGIN      
        1 agcaaaacaa ttgatggttg taatgcaaat aatagcagta ataatttttc attattcaat
       61 tcagaatatg tatttcacaa ttggattgat aattttgtta ttttttgttg caagatacgt
      121 catttcctta cctagtacta ttcctcctca aaattgaaaa attaaataga tttcattggt
      181 tattgcaaag catattagtg ataacattat ggttttatat tttgggaaat tagtggaaat
      241 gaattccagt aaagaattat tttcaaatcc tctccatccc tatactaaaa aaattatttc
      301 ctctgttcct actttcacaa gtttataaaa aaaccaaaaa tagctatttt cttctatttt
      361 ataggaaatt tcagtttgta ttataattgg aatgcatata aatcttcttt catatttacc
      421 acataagatc ttaggaggaa attatgatta ttaaagaaat gcttaaattt ataaaagcaa
      481 cttgcttgac tcttttattt gttggcttta tttcaccagt ttattctgct accataaaat
      541 ggtctatgcc gggtgattca cttacgcttg atcctcatgc gcaaaatgaa ggtccaacac
      601 acatggtttc tagacaagta tatgagagtt tggttactcg aggtctcgat atgtcaatag
      661 agccacaatt agcaacatca tgggaagcta ctgatcctga aacatgggta tttacccttc
      721 gtgatggtgt agttttttca gatggaaaag cactaagagc aagtgatgtt gaatttagta
      781 tcaacagagc gctttcagga gcatcagata tgatagaact aatagaaagt attacttcag
      841 ttaaagctct tagtccaagt actgttgaaa ttaaaactga gggtccaaat cctattcttt
      901 taaaccaatt gtctcaaatt tttattatgt ctgagggatg gtctaaaaag aacggatgtg
      961 aattacctca ggattttaat gcagaagaag caacacattg ttcaagtaat gcgatggga
//
LOCUS       DTVV01000141            2036 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1354545, whole genome shotgun sequence.
ACCESSION   DTVV01000141 DTVV01000000
VERSION     DTVV01000141.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1354545"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(6..812)
                     /locus_tag="EYO86_01665"
     CDS             complement(6..812)
                     /locus_tag="EYO86_01665"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02625.14,HMM:PF13478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIC41774.1"
                     /translation="MSKNKLFLKEALELENNEKPYAIATVINVKGSSSGKVGDKAIFD
                     EKGYRIIGYVGGGCIENRASDAAIETLINGIPKIIEIDLDSDTIAMGIPCGGSMSVLV
                     EPQMNDPVILIRGEGSIVESLCTIAKMLNFKLIIHTSKSHIHTSKSNEELYPNADKII
                     TEGLGVEDIDDNINYFILATHHSNDDKIALEAIRKGIPYVGVIASQKKADLIKEYLSD
                     NNVSGTELKNLYSPIGLDLKATTPEEIALSILSEIVMLENEATGKQMKHL"
     gene            complement(805..1410)
                     /locus_tag="EYO86_01670"
     CDS             complement(805..1410)
                     /locus_tag="EYO86_01670"
                     /inference="COORDINATES: protein motif:HMM:PF12804.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIC41775.1"
                     /translation="MNISATILAAGSSERMEEANKLLLPYRSEALIKIVSKMLIDSLL
                     DPIFIVTGYEHDKVVKLLPKSLDNIIYNKDWRKGMSRSIYKAISSLPRHIDGNMIVLG
                     DMPLITVKTLNELKSVFLSNNGEKIIYAEYFGKQANPVIFPKKYFKEMLLLKGDNGCK
                     DIISQNRKNTLGVSVDSSEVIFDCDTKGDYSDLVSKISTNV"
     gene            complement(1433..>2036)
                     /locus_tag="EYO86_01675"
     CDS             complement(1433..>2036)
                     /locus_tag="EYO86_01675"
                     /inference="COORDINATES: protein motif:HMM:PF02738.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIC41776.1"
                     /translation="QNAFANEVFIDELAYKAGVDALEFRITMLNNSPRHKAVLRLAAE
                     KAGWGKHLAKGQGMGLAVHESFGSWSAQVATVTVSKAGELSVDKIVASIDCGTVINPD
                     GVKAQMEGSIVYGLTSTLKGEITIEKGAVAQSNFHDFELLRIDEMPKVEIHIVPSIEP
                     PSGAGEPGLPPVAPAVANAIFNANGKRVRKLPIKSKDLKV"
BASE COUNT          637 a          390 c          273 g          736 t
ORIGIN      
        1 tagactcaga ggtgtttcat ttgttttccc gttgcttcat tttctaacat gactatttct
       61 gataatatac taagcgctat ttcctcagga gttgtagcct ttaaatctaa tccaattgga
      121 gaataaagat tttttaattc agtacccgaa acattattat ctgataaata ttctttaatg
      181 agatcagcct ttttttgact tgcaataact ccaacgtaag gtattccttt tcttattgct
      241 tcaagtgcta ttttatcatc attgctatgg tgagtagcta gaataaaata atttatatta
      301 tcatctatat cttcaactcc taagccttcg gttataattt tatctgcatt tggataaagt
      361 tcttcattgc tttttgaagt atgaatatga cttttagacg tatgaataat taatttaaaa
      421 tttaacatct ttgctatagt acataaagat tcaactatac taccctcccc tctaattaaa
      481 ataacaggat cattcatttg tggctcaaca agtactgaca tactacctcc gcatggaata
      541 cccattgcaa tcgtatcact atcaagatca atttcaataa ttttaggtat tccgttaatt
      601 aaagtttcta tagctgcatc tgaagctcta ttctcaatac agcccccacc aacatatcca
      661 ataattctat aacctttttc atcaaagatt gccttatctc ctactttgcc agatgacgaa
      721 cctttaacat taataacagt cgctatagca tatggttttt cattgttttc tagctctaaa
      781 gcttctttta aaaaaagttt atttttagac attagtagat atctttgata ctaagtcaga
      841 ataatcaccc tttgtatcac aatcaaaaat aacttctgac gaatccactg atactcctag
      901 agtgttcttt ctattttgac taattatatc cttacatcca ttgtcacctt ttaaaagaag
      961 catttcttta aaatacttct ttggaaaaat cactggattt gcctgtttac caaaatattc
     1021 agcatatata attttttcac cattattact taaaaaaact gattttaact catttaaagt
     1081 tttcactgta attaaaggca tatcacctaa aacaatcata ttcccatcaa tatgtctagg
     1141 taatgaagat atagccttat atattgatct agacataccc ttacgccagt ctttgttata
     1201 tatgatatta tctaacgatt taggtaaaag tttaactact ttatcatgtt cgtaacctgt
     1261 aacgataaaa atagggtcta aaagtgaatc aattaacatt ttagaaacaa tcttaattaa
     1321 ggcttcactt ctatatggta acaataactt atttgcttct tccatgcgtt cagaagagcc
     1381 tgcagcaaga attgttgcag agatgttcaa attagagtct taaactaaaa atttaaactt
     1441 ttaaatcttt tgatttaatt ggaagttttc gcactctttt accattagca ttaaaaatag
     1501 cgtttgctac ggcaggtgca acaggaggaa gtccgggttc accagctcca cttggaggtt
     1561 ctatacttgg tacaatatgt atttctactt ttggcatttc atcaattcgt aaaagctcaa
     1621 aatcatgaaa gttagattga gctacggcac ctttttcaat ggttatttcg ccttttaaag
     1681 tagatgttaa tccatataca attgatcctt ccatttgtgc tttaactcca tcagggttga
     1741 tgacagtacc gcagtcaata gaagcaacaa ttttatcaac tgataattca ccagcctttg
     1801 agactgttac tgtagctacc tgtgctgacc aagaaccgaa agattcatgg actgccaatc
     1861 ccataccttg cccttttgct aaatgcttgc cccatccagc tttttcagct gctaacctta
     1921 atactgcttt atgcctaggt gagttattta gcatggttat tctaaattct aatgcgtcta
     1981 cacctgcctt ataagcaagc tcatctatga atacttcatt agcaaaagca ttttga
//
LOCUS       DTVV01000142            1240 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1355255, whole genome shotgun sequence.
ACCESSION   DTVV01000142 DTVV01000000
VERSION     DTVV01000142.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1355255"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            49..756
                     /locus_tag="EYO86_01680"
     CDS             49..756
                     /locus_tag="EYO86_01680"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIC41777.1"
                     /translation="MKEKKRILLKISGESLMGDSNYGLNVSTTKFIANEIKKIFNSNI
                     EICLIIGGGNIFRGIRGAAEGIDRSTSDYMGMLATIMNALSMQSILETIGVPTRVQSA
                     INMSQIAEPYIRRKAVRHLEKNRIVIFAAGTGNPFFSTDTAASLRASEMNCSLIIKAT
                     KVDGIYDEDPVNNTKAKHYKNISYDEVLNKNLKVMDSTAISLAKESQIPILITNINRK
                     NSILESIKGIGKFSKIS"
     gene            765..>1240
                     /locus_tag="EYO86_01685"
     CDS             765..>1240
                     /locus_tag="EYO86_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIC41778.1"
                     /translation="MDSNTLRSRMDKAISHFEKELLSVRTARASSTMLDNLMVEAYGT
                     KTPINQLGNINVSDPNTLTIQVWDTSLLKYIENSIIKSNLGINPQSDGSLIRLPIPKL
                     SEERRFELSKIVSKYGENAKISIRNIRRDTLDDLKFDEKEKIISQDELKKNSSEIQ"
BASE COUNT          516 a          144 c          188 g          392 t
ORIGIN      
        1 aaataatttt gaaattattg attataaatt gattgttttt aattcttaat gaaagaaaaa
       61 aaaagaatat tattaaaaat atcaggagag tcattaatgg gtgactctaa ttatggttta
      121 aatgtttcta caacaaaatt tattgccaat gaaataaaaa aaatttttaa ttctaatatt
      181 gaaatttgct taataattgg aggaggcaat atttttagag gaattagagg cgcagcagaa
      241 ggcatagata gatcaacttc agattatatg gggatgttag caacaataat gaatgcttta
      301 tctatgcaaa gtatactgga aactattgga gtgcctacaa gagttcagtc ggcaattaat
      361 atgtctcaaa tcgccgaacc atatattaga agaaaagcag taaggcactt agaaaaaaat
      421 agaattgtta tttttgctgc tggaactggt aatccatttt tttctactga tacagctgcc
      481 tctttaagag cttctgaaat gaattgttca cttataatta aggcaactaa agttgacggc
      541 atatatgatg aagatcctgt taataatact aaagcaaaac attataaaaa tatttcatac
      601 gatgaagttt taaataaaaa cttaaaagta atggacagta ctgctataag cctagcaaaa
      661 gaatctcaaa tacccatatt aattactaac ataaatagaa aaaattcaat attggaatca
      721 attaaaggta taggaaaatt tagtaaaatt agttaaaaag aggtatggat agtaatacat
      781 taagatcaag aatggacaaa gctatatcac atttcgaaaa agaactatta tctgttagaa
      841 ctgcgagagc ttcatctact atgttggata atttaatggt ggaagcttat ggcactaaaa
      901 caccaattaa tcagcttgga aatattaatg tttctgatcc taatacttta actattcaag
      961 tttgggatac ctctctttta aaatatattg aaaattcaat aattaaatca aatttaggaa
     1021 ttaaccctca atctgacggt tctttaataa gactaccaat acctaaatta agtgaagaaa
     1081 gaagatttga attatctaaa attgtttcta aatatggaga aaatgctaaa atttcaataa
     1141 gaaatattag aagagatact ttagatgatt taaaatttga tgaaaaagaa aaaataattt
     1201 ctcaagatga attaaaaaaa aattcttcag aaatacaaca
//
LOCUS       DTVV01000143            1173 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1361499, whole genome shotgun sequence.
ACCESSION   DTVV01000143 DTVV01000000
VERSION     DTVV01000143.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1361499"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="EYO86_01690"
     CDS             complement(<1..401)
                     /locus_tag="EYO86_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020037179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIC41779.1"
                     /translation="MRRLNLRSLIPNLITLSGLSFGLSSIRFAIEGEFNLAVICILFA
                     AVCDVLDGMLARHLDSESDLGFQLDSLSDFLSFGVAPGILIYMAIFYENSSIGVFATL
                     IFIIFSCLRLALFNVLHEKSKHNEIQRIGFFA"
     gene            complement(398..1069)
                     /locus_tag="EYO86_01695"
     CDS             complement(398..1069)
                     /locus_tag="EYO86_01695"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020037178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIC41780.1"
                     /translation="MSKKNSNTKYYIHPEGWKFAIIFFVISYLISFLYMPASLLGYTL
                     TMFTLWFFRNPKRKTPNIANSIISSADGKVCLIDKACPPEEIGMNANEMLRICVFMNV
                     FNVHINRSPIAGKINKIVYKKGHFFNASLDKASEKNERSSLVLSNEKGVEIVVVQIAG
                     LIARRILSFVSPNQSLDQGERYGLIRFGSRVDIYLPMNSNINCNVGDKVIAGESVLAT
                     LAEGL"
BASE COUNT          417 a          212 c          166 g          378 t
ORIGIN      
        1 gcaaaaaaac ctattctttg aatttcattg tgctttgatt tttcatgcaa gacattaaaa
       61 agagctagtc ttaggcaaga aaaaataata aaaattaaag tagcaaaaac gccaatgctg
      121 ctattttcat aaaaaatagc catatatatt aaaatgccag gagctactcc gaagcttaaa
      181 aaatctgaaa gagagtctaa ttggaaacca agatctgatt cactatcaag atgtctagca
      241 agcattccat caagaacatc acatactgca gcaaatagaa tacaaattac tgccaaatta
      301 aactctcctt caatagcaaa tcttatagag cttaatccaa agcttaaacc tgacaaagta
      361 attaaatttg gtatcaaaga ccttaaattt aatcttctca taagccttca gccaaagttg
      421 cgagaacgct ttctcctgct ataaccttat caccgacatt acagttaata tttgagttca
      481 ttggcaaata aatgtctact cttgacccaa atctgattaa tccatatctc tctccttgat
      541 ctaggctttg attaggcgaa acaaagctga gaattcttct agcaattaag ccagcgattt
      601 gaacaacgac tatttcaact cctttttcat ttgaaagcac gagactactt ctttcatttt
      661 tttcacttgc tttgtctaaa ctagcattaa agaaatggcc ttttttatag acaatcttat
      721 taatttttcc tgcaattggg ctcctattaa tatgaacatt gaatacattc atgaaaacgc
      781 aaatacgaag catttcattt gcattcattc caatctcttc aggaggacat gctttatcaa
      841 ttaggcacac ttttccatct gcagagctaa taattgagtt agcaatgttg ggggtttttc
      901 tttttgggtt tctaaaaaac catagagtaa acattgtcaa tgtataaccc aaaagagagg
      961 ctggcatata caaaaaggat attagatatg aaataacaaa aaaaataatg gcaaatttcc
     1021 atccctcagg atgaatataa tatttagtat ttgaattttt ttttgacatt ttttaatttt
     1081 agttaataga tttactttag tataacgagt taagttctta tgtcccacac aaatgccggt
     1141 ggaaaattta acagtgtgaa cccagtcaac tta
//
LOCUS       DTVV01000144            2373 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1361563, whole genome shotgun sequence.
ACCESSION   DTVV01000144 DTVV01000000
VERSION     DTVV01000144.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1361563"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2109
                     /gene="alaS"
                     /locus_tag="EYO86_01700"
     CDS             <1..2109
                     /gene="alaS"
                     /locus_tag="EYO86_01700"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIC41781.1"
                     /translation="DEEGDRFIEIWNLVFMQFEQIAKEERIKLPKPSIDTGMGLERIT
                     ALLQNTNDNYETDIFSSLINKSIELSNSDENHKSPSHRVIADHLRSSAFLISDGVLPS
                     NEGRGYVLRRIMRRGMRHAHSLGNTEPIFDKIFPILLSNMKDSYPELERAKDLINNTL
                     FNEETKFKQTIDNGLKILEDEIKKTKNNLFNGDVAFKLYDTYGFPLDLTQDYLKNKNI
                     GVDTLTFDKNMIQQKERARKSWKGTGDTENSKIWFDVVKNLEPTEFVGYDQIKSESLV
                     KKIVVSNKEVTKIKTGEEGVVILNQTPFYGESGGQIGDGGNLSNDQFIFEIYNTTKMF
                     GNYFLHWGKVIKGFANINDKVSTSINVKKRDLIRNNHSSTHLLHASLRKILGNHVFQK
                     GSLVNDEKLRFDFSHNLPLSDMELDKIEKLVNKIISQNIKVNTQILDHQSAINEGAIA
                     LFGEKYSEEVRVITMGQDTDSYFSKELCGGTHVNYTGDINKFKIINQYSVASGVRRLE
                     AVSNIGVDNFETIQRAKNKETSFRTQKEIHKYLNLIRKIQPEKKIGINNNMELEEQLK
                     DIKKTYNDIQFDTNISKNKKNIIVKKIGDYNLIYLLATKYPSKAFKLFVDEQKNAYPH
                     KSIIVLVSSDDTKVSIIVGITNDITEKFDATSFVKIASSIVGGNGGGGRKDLAQAGGN
                     EPTNVKKIFDEIKNEILKLS"
     gene            complement(2101..>2373)
                     /locus_tag="EYO86_01705"
     CDS             complement(2101..>2373)
                     /locus_tag="EYO86_01705"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834015.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIC41782.1"
                     /translation="YRNHQKGLHTSTNPIASIFAWTRGLHFRGEFDQNPELIVFADNL
                     EKVCVETVESGKMTKDLAMLISPKQEWLNTEDFLNTLKQRLEKILA"
BASE COUNT          942 a          297 c          401 g          733 t
ORIGIN      
        1 gatgaagaag gggacagatt tatagaaatc tggaatcttg tatttatgca atttgagcaa
       61 attgcaaaag aagaaagaat taaacttccc aaaccttcga ttgacactgg aatgggctta
      121 gaaagaatta ccgctctatt acaaaataca aatgataatt acgaaacaga tattttttca
      181 tcattaatta acaaatcaat tgaattatca aattcagatg aaaatcataa atctcctagc
      241 catagagtaa ttgccgacca tcttcgttca tctgcttttt taattagtga tggcgtttta
      301 ccttctaacg aaggtagggg atatgtttta agaagaatta tgaggcgagg tatgagacat
      361 gctcacagtt taggaaatac tgaacctata ttcgataaaa tttttccaat attgttatct
      421 aatatgaagg attcttatcc tgaattagaa agagcaaaag atttaatcaa caatacttta
      481 ttcaatgaag aaactaaatt taaacaaact atagataatg gattaaaaat tttagaggat
      541 gaaatcaaaa aaacaaaaaa taatttattc aatggtgatg ttgcttttaa attatacgat
      601 acttacggct ttcctcttga tttaacacaa gattatttaa aaaataagaa tattggtgtc
      661 gatactttaa cgtttgacaa aaatatgatc caacaaaaag aaagagcaag gaaaagttgg
      721 aaaggaacgg gagatactga aaacagtaaa atttggtttg atgttgtaaa aaatttagag
      781 ccaactgagt ttgtgggtta tgatcaaata aaatctgaga gtttggtaaa aaaaattgtt
      841 gtaagtaaca aagaggtaac aaaaataaaa acaggagaag aaggtgtagt aattttgaat
      901 caaacaccat tctacggaga atctggaggg caaattggtg atggaggaaa tttatctaat
      961 gatcaattta tttttgaaat ttataacacc acaaaaatgt ttggaaacta ttttttgcat
     1021 tggggtaagg tgattaaggg ttttgcaaat ataaacgata aggtatctac ttcaatcaat
     1081 gtaaaaaaaa gagatttaat acgtaataat cattcatcaa ctcatctttt acacgcatca
     1141 ttgagaaaaa ttttgggcaa ccacgttttt caaaaaggtt ctttggttaa tgatgaaaaa
     1201 ttacgttttg atttcagcca taatttgcca cttagtgata tggaattgga taaaattgaa
     1261 aaactagtta acaaaataat ttcacaaaat attaaagtga acacacaaat attggatcat
     1321 caatctgcta ttaatgaagg tgcaatagca ttgttcggag aaaaatatag tgaagaagtg
     1381 cgtgtcatta caatgggtca agatacagat agttattttt caaaagaatt atgtgggggt
     1441 acacatgtaa attatacagg ggacattaat aaatttaaaa taataaatca atattctgtt
     1501 gcttctggag tacgtagatt agaggctgtt tcaaatatcg gagttgataa cttcgaaact
     1561 atacagagag ctaaaaacaa agaaaccagc tttcgtactc aaaaagaaat acataaatat
     1621 ttaaatttaa taaggaaaat acaacctgaa aaaaaaattg gaataaataa taatatggaa
     1681 ctagaagaac agctaaaaga tattaaaaaa acttacaatg atattcaatt tgatacaaat
     1741 atttcaaaaa ataagaaaaa cattattgtt aagaaaattg gggattataa tttgatatat
     1801 ttactagcaa caaaatatcc aagtaaagca tttaaactat ttgttgatga acaaaaaaat
     1861 gcttaccccc acaaaagtat aatagtttta gtttcttctg atgatacaaa ggtgtcaatt
     1921 attgttggaa taactaatga tataacagag aaatttgacg caactagttt tgttaaaata
     1981 gcctcatcaa ttgttggtgg caatggagga ggggggcgaa aggatctggc tcaagcagga
     2041 ggaaatgagc caactaacgt aaaaaaaata tttgatgaaa ttaaaaatga aattttaaaa
     2101 ctaagctaat attttttcta atctttgctt taatgtattc aaaaaatctt cagtattaag
     2161 ccattcttgt tttgggctga tcagcatagc taaatctttt gtcatttttc ctgattcaac
     2221 tgtttctaca caaacttttt ctaaattatc agcaaaaaca atcagctctg gattttgatc
     2281 aaattcaccc ctgaaatgta aaccccttgt ccaggcaaat atcgaggcta ttggatttgt
     2341 agaagtgtgc aatccttttt gatgattgcg gta
//
LOCUS       DTVV01000145            1424 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1366517, whole genome shotgun sequence.
ACCESSION   DTVV01000145 DTVV01000000
VERSION     DTVV01000145.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1366517"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
BASE COUNT          551 a          188 c          256 g          429 t
ORIGIN      
        1 aacaggggaa cactcactgg aggaagcggt gccactgctt atgatatcga aattatattg
       61 ggtacgacag gaactcgaat tattatagat aaatctccaa cttttacact tggtactatt
      121 gatttaaacg aaactgcaac aacaatcgaa ttaacttgta caattaaaaa agatttaacc
      181 ttaactatta tagctgctca atccgtaacc tacgataata aactttgtgg aaatgatact
      241 tatacaataa caacaagtgg taacaatgat actttaacca ttgatggtaa tgatttggaa
      301 gttgagactg gtaattcaaa atataaatct gaactcgttt taacaaaatt aaggggattg
      361 ttaaacgcag caaattatat tgatgaaaac gcctctgaag ataaattcgt taaagttttt
      421 tacaataccc aaaaaaggga aaacatttac tcaggagata tgcatggaat tgttggtcaa
      481 ctcagcccga ttaaatgggg accgataaca agcaacattt tccttggcta tacaaaacaa
      541 aatggtgatt ttgacgctgg aagcttttta ggaggaagca attatgcctt gggtcttaaa
      601 aatacctatg aaaaaaatgg tattaaagct tcattgaatc caatgattgg atttcaaagc
      661 gtttctataa ctgattatga tatagaagca aaaacatttg catttaatga tctattaagt
      721 gaattcatgg gattgcaggg aaatgttaaa aaagaaatta atatcagcga tgattcatct
      781 ttaaatttaa aagttaaatc tacatatgga gtacaaaaat ttccagatta tgttgcaaat
      841 tttcttgatg gagatgtatc agtcgatgac tctatagacc aagttctggg tggaggtttt
      901 gaagttgaat acaatgagga tttaggaaat gaattcatta ttaaacctta tgttggattt
      961 caatataata atttgaaaaa tcaaacttac ggaattaatg ctgctggaga tagaaaagac
     1021 gtatcaaccg ctgcaacaga tgcatctggt tattatgctg gttttgcact ttcaaaagac
     1081 acaaaacttt ttaatctaga tttaaatgtc gggtttggaa ataccggagg tttgattgat
     1141 caatttacaa gtttttctct tactaaatct tttggtaagg atgtgcttca aaaggctagt
     1201 gttaaaaaag tttctacaac atcaaaaatt gagataagtg acaataccca agaatattca
     1261 aaagaattaa tagaaattaa agagatcagg aaattagctg aaaaattaat gttagaaaat
     1321 aaaaaattac aaactgagaa tgaaaaatta agaatgttta gtgaaaaggt agctacagaa
     1381 agtaaagcaa gcaaaaaact gattgttgcg cttttaaaag aaaa
//
LOCUS       DTVV01000146            1965 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1366711, whole genome shotgun sequence.
ACCESSION   DTVV01000146 DTVV01000000
VERSION     DTVV01000146.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1366711"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="EYO86_01710"
     CDS             <1..339
                     /locus_tag="EYO86_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HIC41783.1"
                     /translation="IITNQSVIGRKIISETKLEEIHLKLKNYLKQYNTYVDSIYYCPH
                     TPEQNCNCRKPKPGLLIKASDDFNIDLGKSYFIGDSEGDLTAASEAECKGILLKKDQT
                     LLEIVKKYFN"
     gene            complement(341..949)
                     /locus_tag="EYO86_01715"
     CDS             complement(341..949)
                     /locus_tag="EYO86_01715"
                     /inference="COORDINATES: protein motif:HMM:PF13580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIC41784.1"
                     /translation="MFGCSIMKINEEIEEYLEKQNDCVSNIRKNQIHIVEEIFECLIK
                     CRESENRIFLIGNGGSASTASHFTSDLLKTAITKDTKRFKAYSLTDNMPVVLAWANDS
                     SYDEVFKNQLESQIEENDVLITLSGSGNSTNVIKANEYANEVSAHTIAFTGKDGGKLA
                     KIAKISLVIPSDDMLTIESMHLLICHLLITMIRKQGKPMFTY"
     gene            complement(961..>1965)
                     /locus_tag="EYO86_01720"
     CDS             complement(961..>1965)
                     /locus_tag="EYO86_01720"
                     /inference="COORDINATES: protein motif:HMM:PF08666.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HIC41785.1"
                     /translation="SYLEIDGKVIGPHKPTYFIAEAGLNHNTDIKLAKKLIEEAHNCG
                     ADAVKFQTFKSEKFLTLTSSYYNGFKQAELSYDKFAELKDFAKSVGITFFSAPFDIES
                     ADFLKKIGVQCFKIASGDFTDLPLIRHVAKMNVPMIISTGVANMEEVEEVVNLCMFES
                     NKKIALLHCIAHYPTDPEEANLLAMDTMRKKFHVPVGYSDNGESLLVDIVAASMGANL
                     IEKHFTIDKKLPGPDHFFSIDPVGLKDLISKIRIVESMHGDGVKAPQISEMEIRQDIR
                     KSITAAEDISENEIITAEKLLTKRPAIGIKPKYFERVVGKKANKKIKKDTAIQWEDIS
                     "
BASE COUNT          622 a          364 c          270 g          709 t
ORIGIN      
        1 attattacaa accaatcagt tataggcaga aagattattt cagaaacaaa attagaagaa
       61 atacatctta aactaaaaaa ttatttgaaa caatataaca catatgtgga ttcaatatat
      121 tactgcccac atactcctga acaaaactgt aattgtagaa agccaaagcc gggtttacta
      181 attaaggcga gtgatgattt taacatagat ttggggaaaa gttatttcat tggggattca
      241 gagggcgact tgactgctgc aagtgaagct gaatgtaagg gaattcttct caaaaaagat
      301 caaacacttt tagaaatagt taaaaaatat tttaattaat ttaatatgta aacattggtt
      361 tgccctgctt tctaatcata gttattaaca aatggcaaat taaaaggtgc atagattcaa
      421 tggttaacat atcatcgctg ggtattacaa gactaatttt tgcaattttg gctagttttc
      481 ctccatcttt tcctgtaaaa gctatagtgt gtgcagatac ctcattcgca tattcatttg
      541 ccttaattac attcgtagaa tttccacttc cacttagagt aattagcaca tcattttctt
      601 caatctggct ttctaattga tttttaaaaa cctcatcata tgacgaatca ttagcccatg
      661 ctaataccac aggcatgtta tcagttaatg aataagcttt gaatcgtttc gtgtcttttg
      721 taatagcagt ttttaaaaga tccgaagtaa aatgagatgc ggttgaagca cttccaccgt
      781 ttccgataag gaaaattcta ttttcgcttt ctctacactt gattaaacac tcaaaaattt
      841 cttctactat gtgtatttga tttttcctta tatttgagac gcaatcattt tgtttctcta
      901 aatattcctc aatttcttcg ttaattttca ttattgaaca tccaaacatt tttcaatttt
      961 ttaagatata tcttcccatt gaattgcagt atcttttttg atttttttat tagccttttt
     1021 accaaccacc cgttcaaaat attttggttt tattccaata gcaggccttt tggtcagtaa
     1081 tttttccgca gtaattattt cattttcaga tatatcctca gcagctgtaa tagatttccg
     1141 tatatcttgt ctgatttcca tttctgaaat ttgtggcgct tttacaccat caccatgcat
     1201 actctcaact attcttattt ttgaaattaa gtcttttagc ccaacaggat caattgaaaa
     1261 aaaatgatct ggaccaggta attttttatc tattgtaaag tgtttctcaa tcaaatttgc
     1321 acccatactt gcagctacaa tatctaccaa taatgattct ccattatctg aataacccac
     1381 tggaacatga aatttttttc tcatggtatc catcgctaac agattagctt cctctgggtc
     1441 tgtaggataa tgcgcaatgc agtgtagcaa tgcaattttt ttattactct caaacataca
     1501 caaattaact acctcctcaa cttcttccat atttgctaca cccgtagaaa ttatcatagg
     1561 cacgttcatt ttagcaacat gccttatcaa cggtagatca gtaaaatcac ccgatgcaat
     1621 tttaaaacac tgtacaccaa ttttttttaa aaaatccgca ctctctatat caaaaggcgc
     1681 tgaaaaaaat gtgataccta cacttttagc aaaatccttt aactccgcaa atttatcgta
     1741 agaaagctct gcctgtttaa atccattgta ataactactc gttagagtta agaatttttc
     1801 tgatttgaat gtctgaaatt ttacggcatc agctccacaa ttatgtgctt cttctatcag
     1861 tttttttgct aatttaatat cagtgttatg attcagtcca gcttcagcaa taaaatatgt
     1921 tggtttatgt ggaccgatca cctttccatc aatttcaaga taaga
//
LOCUS       DTVV01000147            1309 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1369621, whole genome shotgun sequence.
ACCESSION   DTVV01000147 DTVV01000000
VERSION     DTVV01000147.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1369621"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            185..1246
                     /locus_tag="EYO86_01725"
     CDS             185..1246
                     /locus_tag="EYO86_01725"
                     /inference="COORDINATES: protein motif:HMM:PF01343.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIC41786.1"
                     /translation="MTHFFSTFTKILGSLTAILTFIIIIGLIISFINRSSDKSSFTYL
                     QGDKASANKIAILNINGPIISEPTNFYNITGMGNFDAIYPSLIENYLIDLQQEEVLGL
                     IVSINSPGGSVSASHTIYNLFNQFKIKNNIPLYFYSTDMLTSGAYWVSLSANKIFTNY
                     GALIGSIGVKGPDWIYYNSPTSLSTGLFGNAVESPKGIELFSNTAGISKDILNPFRQP
                     SKKEISQLQNMVNNIYNDFVNLVSSNRKIEKNIVVNEIGAMIYNSKEAQKHYLIDGQK
                     NINETIEVMAKELNLDNTNIISNNKKNLFNFQKFNFFMNVLDNKQYTLNENLINKKFC
                     NNIKNEFSVTTFNFYNSMC"
BASE COUNT          491 a          192 c          169 g          457 t
ORIGIN      
        1 agcactctgt cgtgtaatgg tttaaaattc atatgtttcc tcaaattaag ttattgaaaa
       61 ttaatgtttt tttataagca ctctatacca tagagtgcta atcatgtaat catttttaaa
      121 atctattcaa gagccgttta aaaaaatata ttaaataatt aagattaaat agtaagtata
      181 aactatgaca cactttttta gtacctttac aaaaatacta gggtctttaa cagctatcct
      241 gacttttatc attataatag gcctgatcat aagttttata aaccgatcat ctgacaaatc
      301 ttcttttact tacttacaag gagataaagc ctccgctaac aagatagcta tattaaacat
      361 caatgggcct attatttctg aaccaacaaa tttctataat attactggga tgggcaactt
      421 tgacgctata tatccatcct taatagaaaa ttatttaata gacttgcaac aagaggaagt
      481 cttaggccta attgtatcaa ttaactctcc aggaggttca gtatctgcaa gtcacaccat
      541 ttacaattta ttcaatcaat ttaaaattaa aaataacata ccattatatt tttattcaac
      601 ggatatgttg acttctggag cctattgggt gtcattatca gctaataaaa tttttacaaa
      661 ctatggcgct ctaatcggaa gcataggtgt aaaaggtcct gattggatct actacaattc
      721 tccaacttct ttgtccacag gcttgtttgg taatgcagtt gaatctccta aaggtattga
      781 attattttca aatacagctg gaatttcaaa agatattctg aatcccttca gacagccaag
      841 taaaaaagaa atttcccaat tgcaaaatat ggtgaacaat atttataacg attttgttaa
      901 tcttgtatca tccaatcgta agattgaaaa aaacattgtt gtaaatgaaa tcggagccat
      961 gatttataat tcaaaggaag ctcaaaaaca ttatttaatt gatggtcaaa aaaatattaa
     1021 tgaaactata gaagtgatgg caaaagagtt aaatcttgat aacacaaata ttattagtaa
     1081 taataaaaaa aatcttttca attttcaaaa atttaatttt ttcatgaacg tgcttgataa
     1141 caaacagtac actttgaatg aaaatttaat aaataaaaaa ttttgcaata atataaaaaa
     1201 tgaatttagc gttaccactt ttaatttcta taactcaatg tgttaatgtg ctataatttg
     1261 actgactttg tctaatttta ataataaatt tttataataa ttgctttca
//
LOCUS       DTVV01000148            2107 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1379524, whole genome shotgun sequence.
ACCESSION   DTVV01000148 DTVV01000000
VERSION     DTVV01000148.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1379524"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="EYO86_01730"
     CDS             <1..470
                     /locus_tag="EYO86_01730"
                     /inference="COORDINATES: protein motif:HMM:PF02390.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41787.1"
                     /translation="PHAKIITCELFEDGNINLSNEIIKKSIDNIKLFQGNVLEFLDFI
                     KKSKIFNEIWILFPDPWPKARHHKRRLLNINFLELIYSYLKDEGRIFIATDSQTYIQL
                     ILSIIHKVRDYFNWENQRLEEWDYENLDLPETKFFKKAKKSNRKTMFFQLKKI"
     gene            562..>2107
                     /gene="nusA"
                     /locus_tag="EYO86_01735"
     CDS             562..>2107
                     /gene="nusA"
                     /locus_tag="EYO86_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HIC41788.1"
                     /translation="MAKQKIKVIREEMLQVANNVAQEKSIDQDSVFEAMEMALEKAAR
                     VKYGFERDIRVSINRDNGDIKLNSYLEVVAKLSEEEQAKQITLDEAKKIQPNITTGEF
                     IIKELPPIDMGRVAAQNAKGVIIQKVREADKSRQFSEYKDKVGEIAVGIVKRTEFGNL
                     IVDLGKNEAIIKREELIPRETFQNGDRVRAYIYDVKEDIKGYQIFLSRTHPQFLSNLF
                     TQEVPEIYENVIQIKSVARDPGSRAKISVYTEDSTIDPVGACVGMRGSRVQAVVNELQ
                     GEKIDIVMWSDNQATFLANALAPAEVSKIFLYEEKKKVEVVIPDEQLSLAIGRKGQNV
                     KLASNLTNLEIDILTEEQESERRQEEFREKTQMLINNLDVEDVIAQLLVTEGYVSVEG
                     IASESLENLEKIEGFDKNLASELISRASNHLSEKNAEDVKIINEKIHDNELKKLDGMN
                     NKMLSLLAQNNIVTIDDFADLASFELIDKEEGIFKDLDIDENVVNNMIMKAREKWFTE
                     APVEEVA"
BASE COUNT          852 a          214 c          392 g          649 t
ORIGIN      
        1 atccacatgc aaagattata acttgtgaat tatttgaaga tggtaatatt aatttaagca
       61 atgaaataat aaaaaaaagt attgataata ttaaattatt tcaaggcaat gtattagaat
      121 ttcttgattt tataaaaaaa tcaaaaattt ttaatgaaat ttggatttta tttcctgatc
      181 catggccaaa agcaagacat cataaaagaa gacttttaaa tattaatttt ttggaattaa
      241 tatattccta cttaaaagat gaaggtagaa tatttattgc aacagattcg caaacataca
      301 ttcagttaat tttatctatt atacacaaag taagagatta ttttaattgg gaaaatcaac
      361 gattagaaga atgggattat gaaaatttag atttaccaga aacaaaattt tttaaaaaag
      421 ctaagaaatc aaatagaaaa acgatgtttt ttcaattaaa aaaaatataa ttcttgaatt
      481 tatgaggttt ttttaatata cgcgtgtaaa atttaattaa gttcgaaggt gggattaccc
      541 gccttttttt tggtaaataa catggctaaa caaaaaatta aagtaataag agaagaaatg
      601 ctacaagttg ctaataatgt ggcacaagaa aaaagtattg atcaagattc tgtttttgaa
      661 gcaatggaaa tggctttaga aaaagcagct agagtcaaat atggctttga aagagatata
      721 agagtttcta taaatcgtga taatggtgat ataaaattaa attcttacct agaagtagta
      781 gcaaaactaa gcgaagaaga acaagcaaaa caaattacct tagatgaagc aaaaaaaatt
      841 caacctaata ttacaactgg agaatttatt atcaaagaac tacctcctat tgatatggga
      901 agagttgctg cgcaaaatgc taaaggcgta attattcaaa aagttagaga agcagataaa
      961 tcaagacaat ttagtgagta taaagataaa gttggtgaaa ttgctgttgg gattgttaaa
     1021 cggactgaat ttggaaactt aatagtagat ttagggaaga acgaagctat tataaaaagg
     1081 gaagaattaa taccaaggga gacttttcaa aatggtgata gagttagagc ttatatttac
     1141 gatgtaaaag aagatattaa ggggtatcaa attttcttat cacgtactca tcctcaattt
     1201 ttgtctaacc tttttactca agaagtgcct gaaatttatg agaatgtaat tcaaatcaaa
     1261 agtgtagcta gggaccctgg ttcaagagca aaaattagtg tttatactga agattctact
     1321 attgatcctg tgggagcatg tgtaggaatg agaggttctc gagttcaagc cgttgtgaat
     1381 gaattacaag gagaaaaaat tgatattgtt atgtggtcag ataatcaagc aactttttta
     1441 gctaatgctt tggctcctgc tgaggtgagt aagatctttt tatacgaaga aaaaaaaaag
     1501 gttgaagtag taatacctga tgaacagtta agtttagcaa ttggtcgaaa aggtcaaaat
     1561 gttaaattag catctaattt aactaattta gaaattgata ttcttactga ggaacaggaa
     1621 tcggaaagaa gacaagagga gtttagagaa aaaactcaaa tgttaataaa taatttagat
     1681 gtagaagatg ttattgcgca gcttttagtg acagaaggct atgtatcggt tgaaggaatt
     1741 gcatctgaaa gtttagaaaa tttagaaaaa attgaaggtt ttgataaaaa tttggcaagt
     1801 gaattaattt ctcgtgcttc aaatcacttg agtgagaaaa atgctgaaga tgttaaaatt
     1861 attaatgaaa aaattcatga taatgaatta aaaaaattag atggcatgaa caataaaatg
     1921 ttatcattgt tagctcaaaa taacattgtt acaattgatg attttgcaga tctggcatct
     1981 tttgaattaa tcgataaaga agagggtata ttcaaagatt tagatataga tgaaaacgtt
     2041 gtgaataata tgataatgaa agcaagagaa aaatggttta cagaagcgcc ggtagaagaa
     2101 gttgcga
//
LOCUS       DTVV01000149            1252 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1381707, whole genome shotgun sequence.
ACCESSION   DTVV01000149 DTVV01000000
VERSION     DTVV01000149.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1381707"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="EYO86_01740"
     CDS             <1..577
                     /locus_tag="EYO86_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013960455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC41789.1"
                     /translation="VGTLAGYGLARTNSMMAFWLLIAALVFRALPHTVLVTGYLEPFI
                     NYGLYGKRTAVIILLVAINQPFTIWMMRSFFMNIPQDLDEAAQIDGCNPFQAFWKVIM
                     PLMWPGVITTGLFSFLLAYNDYLVASLLLDGQSQTMVPAIIQYFNMETKMTDLVEAIA
                     AAASITAPLFLLVMFFQKQIISGLTTGAVKG"
     gene            592..>1252
                     /locus_tag="EYO86_01745"
     CDS             592..>1252
                     /locus_tag="EYO86_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41790.1"
                     /translation="MNKDFRFFDSRQKYLLFVTTTNEKSIVAEKISNQIKNIKPKKPA
                     LNILDAGLGDGTLLMSVLRGCHREFPTIPFLVFGKEISMEDVRLSVEKLPDRFVEHPN
                     MVFVVSNLYYSEIASLNSSNPDKQQHINWEIIKLKGSSSFEFSQQFSQLDEMLYNNWQ
                     VERHPKSGNPTYKSPTVLVIYRKDYGFSLDHIIPQQDGSKNYFDLIIASQPYRSRISV
                     QK"
BASE COUNT          396 a          215 c          229 g          412 t
ORIGIN      
        1 tgtaggtact cttgctggtt acggattagc acgtaccaat tcaatgatgg ctttttggtt
       61 attaattgct gcattagttt tccgtgctct tccacacaca gttttggtca caggatattt
      121 agaacctttt ataaattacg gcttatatgg aaaacgtact gcggttataa ttttattagt
      181 tgcaatcaac caacctttta caatttggat gatgagatct tttttcatga atattccaca
      241 agatttagat gaggcagctc aaattgatgg atgcaatcct tttcaagcct tttggaaggt
      301 tattatgcca ctaatgtggc ctggtgttat aactacagga ttattctcgt ttctgttggc
      361 ctataatgat tatttggtag catcattact tctagatggt cagtctcaaa ctatggttcc
      421 agctattatt caatacttta atatggaaac gaaaatgact gatttagttg aggcaattgc
      481 tgcggcagca tcaattacgg ctcctttgtt tttacttgtt atgttttttc aaaaacaaat
      541 tataagtggt ttaacaactg gtgccgttaa aggataaata aaatgataac tgtgaacaaa
      601 gatttcagat tttttgatag tcgccaaaaa tacttattat ttgtgacaac aacaaatgaa
      661 aaaagtatcg ttgcggaaaa aatttcaaat caaataaaaa atatcaagcc aaaaaaacca
      721 gctttgaata ttttagatgc aggacttggg gatggaacat tgctgatgag tgttctaaga
      781 ggttgtcacc gtgaatttcc caccattcct tttcttgtgt ttggaaagga aatcagcatg
      841 gaagacgtgc ggttgagtgt agaaaaatta cccgatcgat ttgtggaaca ccccaacatg
      901 gtttttgtcg tatccaattt atactattct gagatagcat ctctaaattc cagcaaccct
      961 gacaaacaac agcatatcaa ctgggaaatt attaaactca aaggtagttc atcttttgaa
     1021 tttagccagc aattcagtca attagatgag atgttgtata acaactggca agtggaacgg
     1081 caccctaaaa gtggcaatcc aacatataaa tcaccaactg tacttgtcat ctaccggaag
     1141 gactatggat ttagcttgga tcatattatt ccccaacaag atggttccaa aaattatttt
     1201 gatttgatta ttgcatccca accttatcgt tcgcgtataa gtgtacaaaa aa
//
LOCUS       DTVV01000150            1740 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_138357, whole genome shotgun sequence.
ACCESSION   DTVV01000150 DTVV01000000
VERSION     DTVV01000150.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_138357"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="EYO86_01750"
     CDS             <1..218
                     /locus_tag="EYO86_01750"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737513.1"
                     /note="catalyzes the reduction of adenosine
                     5'-phosphosulfate to AMP and sulfite; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylylsulfate reductase subunit alpha"
                     /protein_id="HIC41791.1"
                     /translation="WELKHRHRTSECVVQHTLFREETRWPGYYYRGDKMKLDDENWHV
                     LTTSHRDRVTGEYKMEKQPLYHLIDEK"
     gene            327..677
                     /locus_tag="EYO86_01755"
     CDS             327..677
                     /locus_tag="EYO86_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019558389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41792.1"
                     /translation="MDILDKTDKELLDIARPMWDDLVKFSNNANYGAFTRNFSEEMLR
                     GANEIEMGKQFARSELTKNLNEEVEFLGTIRRGDHATVLFKQKHKKKPGEWLGRLVLG
                     YEDDEIKIFGATIF"
     gene            684..1001
                     /locus_tag="EYO86_01760"
     CDS             684..1001
                     /locus_tag="EYO86_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41793.1"
                     /translation="MSNMIIEDGKFVELTYEVVDKKTSEVLSAVEFPLGYIQGISEIL
                     SPEVTAGLVGQVEGDIVELPVDVDQIYGPRDESLVTTMPLDSLDEDLRKVGTMIPMEN
                     IHI"
     gene            1053..1361
                     /locus_tag="EYO86_01765"
     CDS             1053..1361
                     /locus_tag="EYO86_01765"
                     /inference="COORDINATES: protein motif:HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41794.1"
                     /translation="MKNSIESIYEKKIKNADFKGKITIDIKDDSDYIYATIVDNGVGF
                     EKVDKTKMLTPYFTTKKQGAGLGLAIVTKIINDHNSTILFTSIKNGAKVEVILPKYYD
                     "
     gene            1354..>1740
                     /locus_tag="EYO86_01770"
     CDS             1354..>1740
                     /locus_tag="EYO86_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIC41795.1"
                     /translation="MTNEILVIDDNPDIRRLISGILKDKGFVVREAANFDQAMLEINK
                     KLPDVAIIDVKLDKGDNDGIELLVHLKRIDDDVPVIMISGHANVQMAVDSLKLGAFEF
                     IQKPFSSERLINFTTRAAENINLKKEK"
BASE COUNT          617 a          230 c          357 g          536 t
ORIGIN      
        1 catgggagct taagcaccgt caccgtactt ctgagtgtgt tgttcagcat acattattcc
       61 gtgaagaaac tcgctggcct ggttactact accgtggtga taagatgaag ctagatgatg
      121 aaaattggca tgtattgaca acttctcatc gcgatcgcgt tactggtgaa tacaaaatgg
      181 aaaaacaacc gttgtatcac ttaattgacg agaagtaaga aaaacttgct taataaaggc
      241 ctagtaataa gtctttattg aaaaatttaa aaaaccaaca taagtttatg ttggtttttt
      301 tacttctata aatggagaaa tataaaatgg atatacttga taaaactgat aaagaactat
      361 tagatatagc acgtccaatg tgggatgatt tggttaaatt ttctaacaat gcaaattatg
      421 gtgcatttac tagaaatttt tcagaggaaa tgcttcgtgg tgcaaatgag attgagatgg
      481 gtaaacagtt tgccagaagc gaattaacca agaatttaaa tgaagaggtt gagtttcttg
      541 gtacgattcg tcgtggtgac catgctactg ttttgtttaa acaaaagcat aaaaagaagc
      601 caggtgaatg gttaggtcgt ttagtacttg gttatgaaga cgatgaaata aagatcttcg
      661 gcgccactat tttttaatgc attatgagta atatgattat tgaagatgga aaatttgttg
      721 agctaactta tgaagtcgtt gacaaaaaaa ccagtgaagt gttgtctgca gtcgagtttc
      781 cattaggtta tatacaaggt attagtgaaa tcttatcacc tgaggtaaca gctgggttag
      841 taggtcaagt agagggtgat attgttgaac ttcccgttga tgtagaccaa atatacggcc
      901 ctagggatga atctttagta actaccatgc cgcttgatag tctagatgaa gatttaagaa
      961 aagttggcac catgatccca atggaaaata ttcacatcta gaactgtgtt gttttggact
     1021 ctggagtgcg tgaatgtaat gcggttggat aaattaaaaa ttcaatcgag tctatctatg
     1081 aaaaaaaaat caaaaacgct gattttaaag gaaaaattac catagatatc aaagatgata
     1141 gtgactatat atacgctaca attgttgata atggagtagg tttcgaaaaa gttgataaaa
     1201 caaaaatgct cacaccatat tttacaacga aaaaacaagg agcaggtcta ggcttggcta
     1261 tagttactaa aattataaac gatcataata gtacaatttt atttacttct attaaaaacg
     1321 gagcaaaggt agaagttata ttaccgaaat attatgacta acgagatttt agttatcgat
     1381 gacaatcctg atataaggcg actgatttct ggtattttga aagataaggg ctttgtggta
     1441 agagaggctg ctaattttga tcaagctatg ttggaaatta ataaaaaatt acccgatgta
     1501 gcaattatag atgttaaatt agacaaaggt gataatgatg gtattgagtt attagttcat
     1561 ttgaagagaa ttgatgacga tgtgccagtt atcatgatat ctggccatgc caatgttcaa
     1621 atggctgtag attcattaaa acttggagct tttgaattta tacaaaaacc tttttcatct
     1681 gagaggttaa taaattttac aactagagct gctgaaaata taaaccttaa aaaagaaaaa
//
LOCUS       DTVV01000151            1266 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1388331, whole genome shotgun sequence.
ACCESSION   DTVV01000151 DTVV01000000
VERSION     DTVV01000151.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1388331"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="EYO86_01775"
     CDS             complement(<1..288)
                     /locus_tag="EYO86_01775"
                     /inference="COORDINATES: protein motif:HMM:PF01073.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIC41796.1"
                     /translation="MKTVVIFGGSGFIGQHIIRRIAKIGYRIIIPHQQQTNEAKLRLL
                     GVIGQIIPLRFRSFNETIIQRQLQKANVVINLKTLWDEKKISFKDGIFNFNK"
     gene            355..966
                     /locus_tag="EYO86_01780"
     CDS             355..966
                     /locus_tag="EYO86_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIC41797.1"
                     /translation="MKIKLHKNDLPNSLKLGNAIAIDTEAMGLNHQRDRLCLVQLSNG
                     KDTCHLVQINNIKNKPKNLIALLQNKKILKIFHFARFDVGILKHTYGINIQNIYCTKI
                     ASKLTRTFTDKHGYKDLCEELLGIQILKTEQTSDWGSNKLTHNQLQYAATDVLHLHKI
                     KEKLDSMLVRENRIKIAKACFDFVEHRTNLDLMGWDDLDIFRH"
BASE COUNT          447 a          196 c          212 g          411 t
ORIGIN      
        1 cttattaaaa ttaaaaatac catctttaaa acttattttt ttttcatccc atagtgtttt
       61 aagattaata acgacatttg ctttttgaag ttgtctctgt attatcgttt cattaaatga
      121 tctgaatctc aaaggtataa tctgaccaat tactcccaat aatctaagtt ttgcttcatt
      181 tgtttgttgt tgatgcggga tgataattct atatccaatc ttagctattc ttcgaattat
      241 gtgttgacca ataaagccgg atcctccaaa aattactact gttttcatga tttttttatt
      301 gataaatgat atagcactgt tttaataaat catagatcaa taaactgata aataatgaaa
      361 attaaattac acaaaaatga cttaccaaac tcattgaagt tagggaacgc tatagctatt
      421 gatacagagg caatgggtct taaccatcaa agagataggt tgtgccttgt gcagctatct
      481 aatggtaaag acacttgtca tttagtacaa ataaacaaca taaaaaataa acctaaaaat
      541 ttaattgcac tattacaaaa taaaaagatt cttaaaattt ttcattttgc aagatttgat
      601 gtaggtattc ttaaacatac ctatggaatt aatattcaaa atatttattg tacaaaaata
      661 gcttcaaaat taacaagaac ttttaccgat aaacatgggt acaaggactt atgtgaggaa
      721 ttgctgggaa tacaaattct aaaaacagag caaacatcag attggggttc aaataaatta
      781 acacataatc agctacaata tgctgcgacg gatgttcttc atcttcataa aatcaaggaa
      841 aagttagata gtatgttggt tcgggaaaat agaattaaaa ttgctaaggc atgttttgat
      901 tttgttgaac acagaacaaa tttggatctt atgggctggg atgatttaga tatttttagg
      961 cattaataat gaacagagca caaataattt ctgaagcaaa aaaagtagta tctactgaaa
     1021 tcagccatga tattaagact ataaacctgc acgaggcggg gctcaagctt tggttgatct
     1081 cgccaagatg ggattagtgc ataaagaaag gagaggccgc agggttttct atttttttga
     1141 ggactctgaa acctctccgc ctcaatcacc aatagcggca ttatcgggta cacgaacaat
     1201 cgtttacttt aaagacattg atggccagat tatggcctcg gtgacgtctc ttcctggatg
     1261 cttcac
//
LOCUS       DTVV01000152            3425 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1390747, whole genome shotgun sequence.
ACCESSION   DTVV01000152 DTVV01000000
VERSION     DTVV01000152.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1390747"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="EYO86_01785"
     CDS             <1..825
                     /locus_tag="EYO86_01785"
                     /inference="COORDINATES: protein motif:HMM:PF01751.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIC41798.1"
                     /translation="NKKESIKLLETLIERSLLRTDLSKFKELERKTVKEKNKLPGKLV
                     DCSSKKIEGTELFIVEGDSAGGSAKQARFREKQAILPLRGKILNVYSVSLSKIAGNSE
                     IQNLIQAIGCGFGKNFQLSNLRYEKIILMTDADVDGSHIATLLITFFYKYMRKIIEKG
                     KLYLAMPPLYKISYKDSDYFAYDEKQKNKILQSQAKKTSPQITRFKGLGEMPANQLRD
                     TTMDTDHRQLIQIHLKHGAKEEEKTEKLFYSLMGKKAEHRYNFIQENANFTSNLDI"
     gene            833..2317
                     /gene="glpK"
                     /locus_tag="EYO86_01790"
     CDS             833..2317
                     /gene="glpK"
                     /locus_tag="EYO86_01790"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIC41799.1"
                     /translation="MRKFILTIDQGTTSSRVTVYNNRFNVVDTIKKEFTQFFPNDGWV
                     EHNALEIWNDVKALISKILKKNKLKGSQILSIGITNQRETTVLWDKITGKPIYNAIVW
                     QDRRTTGICSALKKKMLVSKIQKITGLIIDPYFSATKIKWILTNAKNAKKLLKKNNLL
                     FGTIDTWLLWNLTKGKSHLTDITNASRTMLFDSKKEKWSKELLSVLKIPSKILPKVVE
                     NTFDFGETNLFGETIKIGGMAGDQQAATIGQACFHSGQSKSTYGTGCFLLMNIGSQFK
                     LSKNRLLTTVAYKIGTKKMYCYEGSIFVAGSAIQWLRDKLLFFKNAKETEKLYSKANI
                     NEKLIIVPALTGLGAPHWKPNARGIIIGITRNTSIPDMVKATIDSITFQTYDLVSAME
                     KDSASNIKEMRVDGGMVTNNNFIQSLSNTLQKKIIKPKNIETTSLGVAYLAALSCGLL
                     KNTSAITSLWESSKIFKPKIKKSLMTNKINEWQKIVSILTKNYP"
     gene            complement(2292..3131)
                     /locus_tag="EYO86_01795"
     CDS             complement(2292..3131)
                     /locus_tag="EYO86_01795"
                     /inference="COORDINATES: protein motif:HMM:PF00117.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIC41800.1"
                     /translation="MNILIVDGNEKVSSEKYTELGMLTQYEVYQEVLEKISAYELNIS
                     IIHPTWGDDFLPPGTNLEDFDGIAWTGSVLNIYDLRPDVRRQIDLAKILLQKKNMIFG
                     SCWGLQVLATAAGGHVRKNPKGLEAVIAKKITLNKQGINHPMYNDKPYTFDSFCWHYD
                     IVEKLPANSIVLSSNDNSDYQSVTFSRNNSEVWGVQYHPEFNSKWISGLISQRKSILL
                     EEDFFSSEHDFNIMYNYLSDLNKYDSLRKDLEISDTLIDDKIRYRELINWLTHLKDNF
                     SSI"
     gene            3195..>3425
                     /locus_tag="EYO86_01800"
     CDS             3195..>3425
                     /locus_tag="EYO86_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HIC41801.1"
                     /translation="MSIKLGIAPIAWSNDDMPELGGDTPIEQCLEEASLAGFTGIELG
                     GKFPRNPGKIKFLLQKYKLSLPGGWYGSLLRER"
BASE COUNT         1289 a          516 c          548 g         1072 t
ORIGIN      
        1 aataaaaaag aatctataaa attgttagaa actttaattg aacgttcgtt actgaggaca
       61 gacttgtcaa aatttaaaga acttgagaga aagactgtta aagaaaaaaa taaacttccc
      121 ggcaaacttg ttgattgctc cagtaaaaaa attgagggaa ctgaattatt tatcgttgaa
      181 ggggatagtg ctggtggctc tgcaaaacaa gcacgtttta gagaaaaaca agctatattg
      241 cctttaagag gcaaaatatt gaatgtatat agtgtctccc tatcaaaaat tgctggtaat
      301 tctgagattc aaaatttaat acaagctata ggatgtggct ttggaaaaaa tttccaatta
      361 tccaatttaa gatatgaaaa aataattctc atgaccgatg cagatgtaga tggctctcac
      421 attgctacgt tattaattac ttttttttat aaatacatgc gcaaaataat tgaaaaggga
      481 aagttatatt tagcaatgcc cccactatat aaaattagtt ataaagattc agattacttt
      541 gcttatgatg aaaaacaaaa aaataaaata cttcaatctc aagcgaaaaa gacgtcccca
      601 cagattactc gttttaaagg ccttggggaa atgcccgcaa accaattacg ggacactaca
      661 atggatactg atcatcgaca attaatacaa attcatttaa agcatggggc caaagaagaa
      721 gaaaagactg agaaactttt ttattcctta atgggcaaaa aagctgagca taggtataat
      781 tttatccaag agaacgctaa ttttacaagc aatttagata tttaacttaa ttatgcggaa
      841 atttatccta acaatagatc aaggtactac gagttcacga gttacagtct ataacaatag
      901 atttaatgtc gttgatacaa taaaaaaaga atttacacag ttttttccaa atgatggatg
      961 ggttgaacat aacgccttag agatatggaa tgatgttaaa gcgttaatat caaaaatctt
     1021 aaaaaaaaat aaactaaaag gatcgcaaat attatctatt ggcatcacaa atcaaagaga
     1081 aacaactgtt ttatgggaca agataaccgg caaacctatt tacaatgcaa tagtttggca
     1141 agaccgaaga actactggga tttgttcagc tctaaagaaa aaaatgttag ttagtaaaat
     1201 tcaaaaaatt actggactaa ttatagatcc atatttttct gcaacaaaaa taaaatggat
     1261 attaaccaat gcaaaaaatg caaaaaaact tttaaaaaaa aataatttgt tatttggaac
     1321 aatagataca tggttacttt ggaatttaac aaaggggaaa tcgcacttaa ctgatattac
     1381 aaatgcttct agaactatgt tatttgattc taaaaaagaa aaatggtcaa aagaattatt
     1441 atctgtctta aaaattccat ccaagatatt gcctaaagta gttgaaaata catttgattt
     1501 tggagaaaca aacctctttg gagaaactat aaaaattggt ggtatggcag gcgatcaaca
     1561 ggcggcaaca attggtcaag catgttttca ttcaggacag tcaaaaagca catatggcac
     1621 aggttgtttt cttttgatga atattggtag tcaatttaag ttatcaaaaa atcgcctcct
     1681 aactacagtt gcctacaaaa ttggtacaaa aaaaatgtat tgctatgaag gaagtatttt
     1741 tgttgctgga tctgctattc aatggttgcg tgataaatta ctatttttta agaatgcaaa
     1801 agaaactgaa aaattatatt caaaagcgaa cattaatgaa aaactaatta ttgtacctgc
     1861 tttaacaggg ctaggtgccc cccattggaa acccaatgca agaggaataa taatcggcat
     1921 aactagaaac acatcgatac cagatatggt caaagcgaca atagatagta ttacttttca
     1981 aacttatgat ttagtcagtg ctatggaaaa agattcagca tctaatatta aggaaatgag
     2041 agtggatgga ggcatggtca caaataataa ctttattcaa agtttgtcta atactttgca
     2101 gaaaaaaatt ataaaaccta aaaatattga aacaacctct ttaggtgttg cttatttagc
     2161 tgcattatct tgtgggcttt taaagaatac gtcagccatt acaagtttat gggaaagtag
     2221 taaaattttt aaaccaaaaa ttaaaaaatc attaatgact aataaaatca atgaatggca
     2281 aaagattgtc tctatattga cgaaaaatta tccttaagat gtgtcaacca attaattaat
     2341 tctctataac gaattttatc atcaattaac gtatcggata tttctaaatc ttttcttaat
     2401 gaatcatatt tattcaaatc agacaaataa ttatacatta tattaaaatc atgctctgaa
     2461 gaaaaaaaat cttcttctaa aagaattgat tttctctggg aaatcaagcc agatatccac
     2521 ttactattaa attctgggtg atattgaact ccccaaactt ctgaattatt tctagagaaa
     2581 gtgacagact gataatcact attatcatta gaagataaaa ctattgaatt tgcaggcaat
     2641 ttttctacta tatcataatg ccaacaaaat gaatcaaaag tatatggttt atcgttatac
     2701 attgggtggt taatgccttg tttatttaat gttatttttt ttgctataac tgcttctaat
     2761 cctttaggat tttttcgtac atgacctcct gcagcggtag ctaatacttg taacccccaa
     2821 caacttccaa aaatcatatt ttttttttga agtagtatct ttgccaaatc aatttgacgt
     2881 cgaacatctg gtcttaaatc ataaatatta agtacgctac cagtccatgc tattccatcg
     2941 aaatcctcta agttagttcc tggcggaaga aaatcatctc cccatgtagg atgtataatg
     3001 ctgatattta attcataagc tgatattttc tctagaacct cttggtaaac ttcatattga
     3061 gttaacattc caagttccgt atatttttca gaactgactt tctcgttgcc atctactatg
     3121 agaatattca ctgaagattt aattaaatta atccttttaa ttttgcaaat cgtaaaataa
     3181 acatgttcaa gttaatgagt ataaaacttg gtatagctcc tattgcctgg agcaacgatg
     3241 atatgcctga gctaggtggt gacacgccta ttgaacaatg tcttgaagaa gctagcttgg
     3301 caggatttac tggaattgaa cttggtggaa aatttccaag aaatcctgga aaaattaaat
     3361 ttcttttaca aaaatacaaa ttatcacttc ctgggggctg gtatggatct ctattacgcg
     3421 aaagg
//
LOCUS       DTVV01000153            1726 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1391540, whole genome shotgun sequence.
ACCESSION   DTVV01000153 DTVV01000000
VERSION     DTVV01000153.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1391540"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="folP"
                     /locus_tag="EYO86_01805"
     CDS             <1..611
                     /gene="folP"
                     /locus_tag="EYO86_01805"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIC41802.1"
                     /translation="EIKKLEPILKKIPKKKFIISIDTNKIETQEFALSQGVHIINDVF
                     GGSEDLFLLTKKYKSGLVLMHTPAPPKTMQSKTHLYTNVISDIKNIFQRKSKLLKKYK
                     IPPSKVWFDPGIGFGKNLQQNLSIMKNIKKFRIEKYGLLIGSSRKSWISGVDKSDVSQ
                     RLGGTIASVLYCLQRGVNIFRVHDVYETRQAIAIFQQIACSK"
     gene            599..943
                     /locus_tag="EYO86_01810"
     CDS             599..943
                     /locus_tag="EYO86_01810"
                     /inference="COORDINATES: protein motif:HMM:PF02152.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIC41803.1"
                     /translation="MFKIEIKNLATKARIGISDKERKKFQLLLVTLQFSYAVKKNDQL
                     NDIQYLKDYSAITKYLKYYIEHSKFKTLEKLVFECSQSLQKKFKLKNVHVSINKVSVA
                     KGYGCESLSVSQ"
     gene            912..1442
                     /gene="folK"
                     /locus_tag="EYO86_01815"
     CDS             912..1442
                     /gene="folK"
                     /locus_tag="EYO86_01815"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIC41804.1"
                     /translation="MAVNLSVYPNRAILALGSNVGNWKTNFNHCLKKINNFGLITRIG
                     NIYVSKPYGHVQQKNFHNTAIELMTNHSPLKLMRGLQLVEKVLKKNRTIINGPRRIDI
                     DIVFYNQLVFQNNQLTIPHPRAFKRDFVLYPINDIDPFYIHPTKHKTIKALIEELNEK
                     YIEKKIKQPRDSLLIR"
     gene            complement(1408..1707)
                     /locus_tag="EYO86_01820"
     CDS             complement(1408..1707)
                     /locus_tag="EYO86_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41805.1"
                     /translation="MEYFDSIKSLVNVFYYDLNVYDKNDITTYSLDDCLNQIMSLKEL
                     KRPVYIGGGINFKNAKKIVDSARPDGLDVSRSLKDGDNNISSTKLNELVTSLLAA"
BASE COUNT          657 a          279 c          263 g          527 t
ORIGIN      
        1 aagaaataaa aaaactagag cctattttaa aaaagatacc gaaaaaaaaa tttatcatat
       61 caattgatac aaataaaatt gaaacgcagg aatttgctct ttcacaagga gtccatatca
      121 ttaacgatgt gtttggtgga agtgaggact tatttttatt aactaaaaaa tacaaaagtg
      181 gcttagtttt aatgcacaca cctgcccctc ctaaaacaat gcagtccaag acgcacctat
      241 atacaaatgt aatcagcgat attaagaata tatttcaacg aaaatcgaag ttactaaaaa
      301 aatataaaat tccaccttct aaggtatggt ttgatcccgg tattggattt ggtaaaaatc
      361 tccagcagaa tttatcaata atgaaaaata taaaaaaatt tcgtattgaa aaatacggct
      421 tgcttattgg ttcatctcga aagagttgga tcagtggggt tgataaatct gatgtaagtc
      481 agcgtttagg cggcaccatt gcatcggtat tgtattgtct tcagagaggg gttaatatat
      541 ttcgcgtgca cgatgtgtat gaaacaaggc aggccattgc aatctttcaa caaatagcat
      601 gttcaaaata gaaattaaaa atttagcgac taaagctcgc attggtattt ccgacaaaga
      661 aaggaagaaa ttccaactct tgttagttac actgcagttc agttacgctg taaaaaaaaa
      721 cgatcaatta aatgatattc aatatttaaa agattattca gccattacaa aatatcttaa
      781 atattacatt gaacactcaa aattcaaaac tttagaaaaa ttggtttttg aatgctccca
      841 atcattgcaa aaaaaattta aattaaagaa tgtccatgta agcattaaca aagtatctgt
      901 tgcgaagggc tatggctgtg aatctctcag tgtatcccaa tagagcaata cttgctcttg
      961 gatcaaacgt gggcaattgg aaaacaaatt tcaatcattg ccttaaaaag attaataatt
     1021 ttggcttaat tactaggata ggtaatattt atgtctctaa accctatggg catgttcaac
     1081 aaaaaaattt tcataataca gcaatagagc tgatgacaaa tcatagtcct ttaaagctta
     1141 tgcgtggttt acaattagtc gagaaggttc ttaaaaaaaa tagaactata atcaatggac
     1201 cgcgacgcat tgacattgat atagtttttt ataaccagct cgtattccaa aataatcaat
     1261 taaccatacc ccatccaaga gcttttaaac gtgattttgt tttatatcca atcaatgata
     1321 tcgatccatt ttatatccat cccaccaaac ataaaacgat aaaagcatta atagaagagt
     1381 taaacgaaaa atatatagaa aaaaaaatta agcagccaag agactcgtta ctaattcgtt
     1441 aagttttgtt gaagaaatat tattatcacc atccttgagg cttcttgata catctagtcc
     1501 atccggacga gcgctatcaa caattttttt tgcattttta aaattaatac cgcccccaat
     1561 atacaccggt cgctttaatt cctttagaga cataatttgg tttaagcaat catcaagaga
     1621 ataggtagta atatcatttt tatcataaac gtttaaatca taataaaaaa catttaccaa
     1681 ggatttaatg gaatcaaaat attccatatc aaacttttta ggattg
//
LOCUS       DTVV01000154            1241 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1392607, whole genome shotgun sequence.
ACCESSION   DTVV01000154 DTVV01000000
VERSION     DTVV01000154.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1392607"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="EYO86_01825"
     CDS             complement(<1..690)
                     /locus_tag="EYO86_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HIC41806.1"
                     /translation="MKCDKCENTAVYSRRYSGENLCSQCFSNSILHKAAKTISKYKMI
                     KNNELVCVAVSGGKDSLVLLDILNKMSKTHNFRIFVVTIDEGIPGYRDEALKIVENFC
                     TQLNIQHKTFSYKKIFDLTLTESLELRNDQKLSSCSICGTFRRRALDHAAKTINADTI
                     ATGHNLDDNLQTFLINILSGDAKKIGWMDPDTSSNKVRKIKPLSEIYESEIVFYAFTN
                     NLPFQTEPCPHM"
BASE COUNT          434 a          192 c          194 g          421 t
ORIGIN      
        1 catgtgtgga catggttcgg tttggaatgg aagattgttt gtaaacgcat aaaatacaat
       61 ttctgattca taaatctcag atagtggttt gatctttcga actttatttg aagatgtgtc
      121 aggatccatc caaccgattt ttttggcatc acctgaaagg atgtttataa gaaatgtttg
      181 gagattatcg tccaagttat gacctgttgc aatagtatct gcgttaattg tttttgctgc
      241 atgatctaaa gcacgtcgtc taaatgttcc acaaattgaa caggaagaca acttctgatc
      301 atttcttagc tctaacgatt ctgtcaaagt aaggtcaaag atctttttgt atgagaaagt
      361 tttatgctga atatttaatt gtgtgcaaaa gttttcaaca attttaagtg cttcatctcg
      421 gtatcctgga atcccttcat caatggtaac aacgaatatc ctaaaattat gagtctttga
      481 cattttattt aaaatatcta ataaaacaag agaatctttt cctccagaaa cagccacaca
      541 aacaagttca ttgtttttaa tcatcttata ctttgaaatt gttttagcag ctttgtgtaa
      601 aatagaattg gaaaagcatt gtgagcaaag attttctcca gaataccttc ttgaataaac
      661 tgctgtgttt tcacatttgt cacatttcat aatttttaaa ataatttatt gttaatatct
      721 tcttgttttc aaaatacagt ctaaaatttg aaataaatga agtcttgcgc aggtccagtt
      781 tcgaatatca aaactaacat cagtatggaa cctaaaaatg aaaaccaata tctaagctta
      841 attctggata ttgcattttg caaatttcca tgtttatatg aaacaaaatt tagaatgaaa
      901 aaaacaataa ttagaataac tggaaactgg ttcaatgaaa ctaactcgat aaattgctcc
      961 gttgcaaagt ctaagaaaac gtatttctgc atagaatacc agatatgatc aaattctgtt
     1021 accctgaatg acaaaaatgc gagaaaaata aaatactgtg ttataattat gctaaaaaat
     1081 ttaccaatgt ttttttgaaa aaatccatgc tgagagattt gaggacattt tcttttaatt
     1141 atcgtatgtg tcgcaaggaa tattccatgt atcgcaccaa agataataaa attccatgat
     1201 gcaccgtgcc aaagacctag caataacatt gagatcatca a
//
LOCUS       DTVV01000155            1236 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1392938, whole genome shotgun sequence.
ACCESSION   DTVV01000155 DTVV01000000
VERSION     DTVV01000155.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1392938"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /gene="mnmG"
                     /locus_tag="EYO86_01830"
     CDS             <1..1166
                     /gene="mnmG"
                     /locus_tag="EYO86_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIC41807.1"
                     /translation="QISCSVTYTNKKLHEIIKKNLLKSAMYSGSIKGVGPRYCPSIED
                     KIVKFSDKTRHQIFLEPEGLNDTTVYPNGISTSLPTNVQEEILSKINGLEHVRMNRPG
                     YAIEYDYVDPRELNHTLEMKKIKSLYLAGQINGTTGYEEAAAQGLMAGINAALSIRDQ
                     ETLVLDRAQAYIGVMIDDLVTKGVAEPYRMFTSRAEYRLSLRADNADRRLTQIGIDIG
                     LVGDERKEKFLKKINKINELDYALTKLKISPNKAEKFNIKVAKDGIKRTAFHILSREG
                     VTFDKIRSIWPELPNASRDEEEQLEISAHYSGYLEKQEADIIAFRKDENLLIPENIDY
                     DKLSGLSNEAKSKFSLIKPKTLGQALRIEGITPAAVYILLSHVKRGHQKFQRA"
BASE COUNT          480 a          180 c          235 g          341 t
ORIGIN      
        1 aacaaatttc ttgcagtgtt acgtacacga ataaaaaact tcatgagata ataaaaaaga
       61 atttattaaa aagtgcaatg tactcaggaa gcatcaaagg agttggacca agatactgtc
      121 catccattga ggataaaatt gtaaaatttt ctgataagac aagacatcaa atttttttag
      181 aacctgaagg tctaaatgat acaacggttt atcctaatgg aatatcaaca tcactaccta
      241 ctaatgttca ggaagaaata ttaagtaaaa tcaatggctt agagcatgtt cgtatgaata
      301 gacctggtta tgcaattgaa tatgattatg ttgatccaag ggaacttaat catactttgg
      361 aaatgaaaaa aataaaatct ctttaccttg ctggtcaaat taatggaaca actggttatg
      421 aagaagcggc agcgcaagga ctcatggcag gtattaatgc agcactatcg attagagatc
      481 aagaaacgtt ggtacttgat agagctcaag cgtacattgg agtgatgata gatgacttgg
      541 ttacaaaagg agtagctgag ccatatagaa tgtttacatc tcgtgctgaa tatagactta
      601 gcctgagggc tgacaatgct gatagacgat taactcaaat tggaattgat attggccttg
      661 taggagacga aaggaaggaa aaatttttaa aaaaaattaa taaaatcaat gaattagatt
      721 atgctttaac aaaacttaaa atttcaccaa acaaagctga aaaatttaat attaaagttg
      781 ctaaggatgg aattaaaaga acagcttttc atattttatc aagggaagga gttacttttg
      841 ataaaattag atctatttgg cctgaattgc ccaatgcatc aagagatgaa gaagaacaac
      901 ttgagataag cgcccactat tctggttatt tagaaaagca agaagcagat ataattgcat
      961 tcagaaagga tgaaaacctt ttaatcccag aaaacattga ttatgataaa ttgagtggcc
     1021 tttctaatga agctaaaagc aaatttagcc ttattaagcc aaaaacccta ggtcaagccc
     1081 ttagaataga aggaattacg cctgcagctg tatatatttt gctttcacat gtaaaaagag
     1141 gacatcagaa gtttcaaagg gcatagatgg aacaatctag ccagatagat acattcagtg
     1201 gaataacaca agtttcacgt gaaacaatca caagtt
//
LOCUS       DTVV01000156            2193 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1394335, whole genome shotgun sequence.
ACCESSION   DTVV01000156 DTVV01000000
VERSION     DTVV01000156.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1394335"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(88..1566)
                     /locus_tag="EYO86_01835"
     CDS             complement(88..1566)
                     /locus_tag="EYO86_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HIC41808.1"
                     /translation="MEEIMPTLSNIGWQFDNSYARLPELMMSRVKPVKVHSPKLIILN
                     QKLSKELGLNFSSLKDRDIASLFAGNSLPQGSETIAQAYAGHQFGHFTMLGDGRAVVI
                     GEHLTPNNKRFDIQFKGSGRTPYSRGGDGKAVLGPMLREYIISESMHALNISTTRSLA
                     VVTTGEEIMRETSLPGAILTRVAASHIRVGTFQFIAMKNDMGTLKNLLNYTINRHYPE
                     LIKENNHAIELLKVVIDTQINLVVDWMRVGFIHGVMNTDNMAISGETIDYGPCAFMDN
                     YDPRTVFSSIDHQGRYCFINQSLIAHWNVSRFAETLIPLLDNNEKKAITIGEEIINSF
                     SEKFQSKWLSMMRQKLGLFGEKNNDEKLVNNLLSLMHQNNTDYTNTFCDLMKNKLPNN
                     SFYQHHKFVDWFKEWKNRQSQNKMPKKLSLSLMRSANPYVIPRNHKVEEILTAASESG
                     NLEPMKKILSELNNPYDYREDINEYRIVPKTSNIQYQTFCGT"
     gene            complement(1747..2067)
                     /locus_tag="EYO86_01840"
     CDS             complement(1747..2067)
                     /locus_tag="EYO86_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41809.1"
                     /translation="MSKNTREFYMICDFTNIIKKTNEITDSKTLCNKIIKDIGFAMLP
                     GSDFGINKELLISRISFVDFDGANALKIVENSKLLNDNFLKLICPNILEGIKKLKEWI
                     IKRN"
BASE COUNT          713 a          377 c          297 g          806 t
ORIGIN      
        1 aatgatgata ttgtattaat taattaaatt ttctaccaat aaatttcatc tccatattca
       61 tattattaga aagaaagact acattcttta tgtgccacaa aatgtttgat attgtatatt
      121 actagtttta ggtacaattc tatattcatt gatatcttct cgataatcat atgggttatt
      181 tagttcactt agtatttttt tcatcggttc caagtttcct gattcacttg ccgctgttaa
      241 tatctcttct accttatgat tacgtggtat gacataagga tttgcagatc gcattaaact
      301 taaagataat ttttttggca ttttgttttg agattgtcta tttttccatt ctttaaacca
      361 atccacaaac ttatgatgtt gataaaaact attattaggc aatttattct tcatcaagtc
      421 acaaaaagta tttgtatagt cagtattatt ttgatgcatt aatgataata aattattaac
      481 aagtttttca tcattatttt tttctccaaa taaacccaat ttttgacgca tcattgataa
      541 ccattttgat tgaaattttt cactaaaact atttattatt tcttctccaa tagtaattgc
      601 ctttttttca ttattatcaa gaaggggtat taaggtttca gcaaatcgtg aaacattcca
      661 atgtgcaatt agtgattgat taataaaaca atatcggcct tgatgatcaa tggagctaaa
      721 aacagttcta ggatcataat tatccataaa tgcacaaggg ccataatcaa tagtttctcc
      781 tgagatagcc atgttgtccg tattcattac tccatgaata aagccaacac gcatccaatc
      841 cacaacaaga tttatttgcg tatcaataac aaccttaagc aattcaatag catgattatt
      901 ttcttttatt aattctgggt aatgtctatt aatcgtataa tttaataaat tcttcaaagt
      961 ccccatatca tttttcattg caataaattg aaatgttcca acacgtatgt gacttgcagc
     1021 tactctagtt agtatagcgc cgggtaagga agtctctctc ataatttcct cccctgtagt
     1081 aactactgca agacttctag tagtagaaat attcaatgcg tgcattgatt cactaattat
     1141 atattctctc aacataggtc caagaacagc cttcccatca cctccgcgag agtagggagt
     1201 tcgaccagat cctttaaatt gaatatcaaa acgtttattg ttaggagtta gatgttcgcc
     1261 tattacgaca gcccttccat caccaagcat ggtaaaatgt ccgaattgat gtcctgcata
     1321 agcctgtgcg atagtttctg aaccttgagg taatgaattc ccagcaaata gagaagcaat
     1381 atccctatct tttaaagaac taaaatttaa gccaagttct tttgatagtt tttgattaag
     1441 aataattagt ttaggagaat gaacttttac tggtttaact cgtgacatca ttaattccgg
     1501 caagcgggca taactattat caaattgcca accaatatta gagagtgtgg gcattatttc
     1561 ttccataaag tactgttttc ttaatattac cttattttaa tcacgaacct gccaaatttc
     1621 aaatggataa tcaagtaagt ggcacaggga cgaaatacgt atttttcata ataatattca
     1681 ttatatttag ttcctcttta tttatgtccc tggccactag aaaaatagtg aaatagaatt
     1741 atatctttaa tttcttttta tgatccattc tttgagtttt tttatacctt ctaagatatt
     1801 agggcaaatt agttttaaaa aattatcatt taataattta gaattttcca ctatttttaa
     1861 tgcattcgca ccgtcaaaat caacaaaaga aattcttgaa atcaataatt ccttatttat
     1921 gccaaaatca gaaccaggta acatggcaaa ccctatatct tttataattt tattacatag
     1981 cgtcttgcta tcggtaattt catttgtttt tttaataata tttgtaaaat cacaaatcat
     2041 ataaaattcc cttgtgtttt ttgacatttg acactaacag aagatagaat cgcatctcct
     2101 agattttaat ttaagggatt ttagggagtt gtaaaatcac cactccatgt cattggccaa
     2161 ccactaccat ctggagagac atttccacca aat
//
LOCUS       DTVV01000157            2051 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1396597, whole genome shotgun sequence.
ACCESSION   DTVV01000157 DTVV01000000
VERSION     DTVV01000157.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1396597"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="EYO86_01845"
     CDS             <1..278
                     /locus_tag="EYO86_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41810.1"
                     /translation="RRFWRITFPLSFPGVLAASMIIFIPTTGDFITPRLVGGSEGIMV
                     ANLIQVMFGKANNWPLGSSLAIITMTIVTLSVICFVVISRWLISRIK"
     gene            275..1099
                     /locus_tag="EYO86_01850"
     CDS             275..1099
                     /locus_tag="EYO86_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC41811.1"
                     /translation="MKATSKFLTGERVLIVYAIFFLLFLYIPVLFLPLFSFSGSQYMT
                     FPITDWTLERYVTMWKKTKLLEALYASIKVGIFASVISTILGILVARAMTRYLFPLKR
                     TVMGFILLPMVFPEIIMGVGLLIFVIFVGMQLSLITVTAGHILICLPFSTVILISRFE
                     GFDRSLEEASLDLGENAWQTFRRITFPIVGSGILASLLLTFTISFDEFLIAFFLSGDD
                     PTLPIYIYGQLRHVKNIPYMLSLGSIILLVSFVTISFAEWIRRRGMSSKKVYGLFE"
     gene            1096..>2051
                     /locus_tag="EYO86_01855"
     CDS             1096..>2051
                     /locus_tag="EYO86_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC41812.1"
                     /translation="MKKHNDFIVVDKITKTFGSVVAVDNIDLTINKGEFFSLLGASGC
                     GKTTLLRLIAGFENPDQGKIFIEGDEITYLPPNKRPTNMVFQNYAIFPHMDVANNIAY
                     GLRKEKFSKKELNLKVDQYLELIKLSGYGNRKAEQLSGGQRQRVALARALIMQPKVLL
                     LDEPLGALDKQLRANMQIELRSLQKDVGITFVFVTHDQEESLSMSDRVAVMSRGKILQ
                     VSSPDELYEKPENEEVANFIGTINFFDATVSTINNEICSFKSKYLGMIQTNCKNNVYS
                     IGEEVLVGIRPEKLNLHVNKPEQDSGIIEGIVENISYLGERS"
BASE COUNT          643 a          319 c          390 g          699 t
ORIGIN      
        1 tcaggcgatt ttggagaata actttccctc tttcattccc aggtgttttg gctgcatcca
       61 tgataatctt cataccaaca acaggagact ttattacccc aagattggta ggtggatcag
      121 aaggtattat ggtagcaaac cttattcagg ttatgtttgg gaaggcgaat aactggccac
      181 ttggatcttc acttgcaatt attacaatga ctattgttac actgtcggtg atttgttttg
      241 ttgtgatatc aagatggctc atatcaagaa taaaatgaaa gctacatcta aatttctaac
      301 aggtgaacgt gtactaatag tatatgcaat atttttttta ttgttcctct atatcccagt
      361 tttattcctt cctctttttt catttagcgg gtctcagtat atgacatttc ccattactga
      421 ctggacgtta gaaagatatg tgacaatgtg gaagaaaaca aaactacttg aagcacttta
      481 tgccagtatt aaagtgggaa tttttgcttc tgtgatcagc actatcctag gcatattagt
      541 agcaagagct atgacaagat acttgtttcc tcttaaacgg acagtaatgg gatttatatt
      601 gttgccaatg gtttttcctg aaatcataat gggtgttggg ctattaattt ttgttatatt
      661 tgtaggtatg caattaagct taattactgt tactgctgga cacatattaa tttgtttgcc
      721 tttttcgact gtcatcttaa tctcaagatt cgaggggttt gacagaagtc ttgaagaggc
      781 ctctttagac ttaggtgaaa atgcttggca aacctttcgt agaattacct ttcctattgt
      841 tggatccgga atccttgcta gtttgttgtt aacttttaca atttctttcg atgagttttt
      901 aattgccttt tttctttctg gagatgatcc cactttacca atctatattt atggacaatt
      961 acgccatgta aaaaatattc catatatgct ctcccttggc tcaatcatcc tactggtttc
     1021 gtttgttaca attagctttg ctgaatggat cagaagaagg ggcatgtcat ccaaaaaagt
     1081 atatggtttg tttgaatgaa aaagcataat gattttattg ttgttgataa aataacaaag
     1141 acgtttggtt cagttgtggc tgttgacaat attgatttaa caataaacaa aggtgaattt
     1201 ttttcattat taggagcttc cggttgcggt aaaacaactt tgttaagatt aatagctggt
     1261 tttgaaaatc ctgaccaagg taaaattttt atagaagggg atgaaataac atacttacca
     1321 cctaataaga gacctaccaa tatggttttt caaaattatg caatttttcc ccatatggat
     1381 gtggctaaca atattgcata tgggttaaga aaagaaaaat tttcaaagaa agaattaaat
     1441 ctcaaggttg atcaatattt agaattgata aaattatctg gttacggaaa tagaaaagca
     1501 gaacaactct caggaggaca aagacaacgt gtggccttgg caagagcatt aattatgcaa
     1561 ccaaaagttt tattgttaga tgagcctctt ggtgctttgg ataagcaatt gagggcaaat
     1621 atgcaaattg aacttcgttc tttacaaaaa gatgtgggga ttacatttgt ttttgtaact
     1681 catgatcaag aagaatcact tagcatgtct gatagagtag cagtcatgtc tcgtggcaag
     1741 atactccaag tttcttcccc ggatgaactg tatgaaaaac ctgagaacga agaagttgca
     1801 aattttattg gaactatcaa tttttttgac gcaactgttt ctaccatcaa caatgaaatt
     1861 tgttcattta aatcaaaata tcttggtatg atacaaacta attgcaaaaa taatgtttat
     1921 tcaatcggag aagaggtcct tgttggaata aggcccgaaa aattaaatct acatgtcaat
     1981 aaaccagaac aggattccgg gataattgag ggtattgtag aaaacatttc ctatttggga
     2041 gagagaagtc a
//
LOCUS       DTVV01000158            1447 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1397018, whole genome shotgun sequence.
ACCESSION   DTVV01000158 DTVV01000000
VERSION     DTVV01000158.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1397018"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="EYO86_01860"
     CDS             complement(<1..148)
                     /locus_tag="EYO86_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIC41813.1"
                     /translation="MVGPTAIGKSSLALRLAEKINGVIINADSMQVYSNLKILTARPS
                     SADLK"
     gene            complement(171..>1447)
                     /locus_tag="EYO86_01865"
     CDS             complement(171..>1447)
                     /locus_tag="EYO86_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIC41814.1"
                     /translation="PSVVSIASTTMVKNRNQQSMPRFPEGSPFDEFFKDYFDKEQRQY
                     PSQRPMIGLGSGFIIDKSGIIITNNHVIEGADEISVIMFDQKKFSAELLGRDPKSDLA
                     VLKINPGDTILQQVSFGDSDKARVGDWTIAIGNPLGLGGTVTAGIVSAISRDIGNGPY
                     VKFIQTDASINRGNSGGPLFNIRGEVIGINTAIISQTGGSIGLGFAIPSNSAIKIVEQ
                     LKEFGRTKRGWLGVQITPVSKEIAESLGLPDEKGAFISNINLDGPSKAAGLQEGDVIL
                     KFNNNEIYKMTDLPRVVAESDVGSTAIIEIWRKNKLITIQVTLGELPEESYVVRKNIR
                     EKKSSDFYLDSLNLTISSTNDKQGVLVIQVDEESSLQIGDIITEVNRELVTNTNNFIE
                     LIETIKKTGRNSLLLKIIRDDKSLWITIQFLK"
BASE COUNT          445 a          284 c          218 g          500 t
ORIGIN      
        1 gtttaaggtc agcagaagat gggcgtgctg ttagaatttt tagatttgag taaacctgca
       61 tgctatcagc gttaatgatt actccattaa ttttctctgc taaacgaagg gctaaagatg
      121 acttgccaat agcagttggc ccaactataa aataaagagt ttggttcaaa ttatttcaaa
      181 aactgaattg taatccatag agatttatcg tctctaataa ttttcaataa taatgaattt
      241 ctcccagttt tttttattgt ttcaattaat tcaataaaat tattagtgtt tgtaacaagt
      301 tcgcggttca cttccgtgat gatatcccct atttgtaagc tagattcctc atcaacctgt
      361 ataaccaaaa ctccttgctt atcattggtt gaactaattg ttaaatttaa actatcaaga
      421 taaaaatcac tagatttttt ttctctaatg ttttttctaa caacatacga ctcttcaggc
      481 aattccccta atgtcacttg aatagtaatt aatttatttt ttctccaaat ttctataata
      541 gctgttgaac caacatctga ttccgcgacc actcttggaa gatctgtcat tttatagatt
      601 tcattattat taaacttaag aattacatca ccttcttgca gacctgcggc cttagatggt
      661 ccgtcaagat tgatatttga tataaaagca cctttttcat caggcaatcc aagactttct
      721 gcaatttctt ttgaaacagg ggtgatttgt acacctagcc atccccgttt tgttcgacca
      781 aactctttta attgttcaac aatttttatt gcactatttg aaggaatggc gaaaccaagg
      841 ccaatgctgc ccccagtttg ggaaattatt gcagtattaa ttccaatcac ctcacctcta
      901 atattaaaaa gagggcctcc agaattaccc cgattaatag aagcatctgt ttgtataaat
      961 ttcacataag gaccattacc tatatctcta gaaatagctg aaacaattcc tgccgtaaca
     1021 gttccgccca aaccaagtgg gttgccaata gcaatagtcc aatcgcccac ccgagcttta
     1081 tctgaatcac caaaacttac ttgttgcaat atagtatcgc ctggattaat ttttaaaaca
     1141 gctagatcag attttgggtc acgtcctaaa agttcagctg aaaatttttt ttgatcaaac
     1201 attattacag aaatctcatc agccccctca ataacgtgat tatttgttat tataattcct
     1261 gatttatcaa taataaatcc tgatcctaag cctatcattg gtctttgtga tggatattgc
     1321 cgctgctctt tatcaaaata atctttaaaa aactcatcaa aaggggagcc ttctggaaaa
     1381 cgaggcattg attgttgatt tctatttttt accattgttg ttgatgctat gctcacaacg
     1441 gagggta
//
LOCUS       DTVV01000159            3086 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1401765, whole genome shotgun sequence.
ACCESSION   DTVV01000159 DTVV01000000
VERSION     DTVV01000159.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1401765"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(120..380)
                     /locus_tag="EYO86_01870"
     CDS             complement(120..380)
                     /locus_tag="EYO86_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41815.1"
                     /translation="MNQSSITIRGKIGLGSLIVNEVEGKLSILSKLIEEKFRFNISKT
                     FKQLPGITGYSISVDLFISSTVKDKTTFILAIFDLFKTQGAC"
     gene            365..1159
                     /locus_tag="EYO86_01875"
     CDS             365..1159
                     /locus_tag="EYO86_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family deoxyribonuclease"
                     /protein_id="HIC41816.1"
                     /translation="MMIDSHCHLNFDNLAIEIENLIRKAKNNNITSILSINTDPDEFD
                     NHYKLIKKFKSIFLSYGLHPQQVTESNIINSAKIINKSSNDRVIAIGETGLDFFHSVK
                     YKKKQYEVFESHIEASFETDLPLIIHQRNSENEIMDVLSNFQKSKSLKVVFHCFTGSS
                     KLRNFCLENGFYLSLSGIITFKNAEELRSVIKNVPLSSVLIETDSPFLTPTPHRGKIN
                     EPSFVKHVAEYLSIFFKVPFEEFISITDNNFYKLFSKAIRYNEISI"
     gene            1156..1908
                     /locus_tag="EYO86_01880"
     CDS             1156..1908
                     /locus_tag="EYO86_01880"
                     /inference="COORDINATES: protein motif:HMM:PF12706.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIC41817.1"
                     /translation="MKIRVLGSGSSFGSPLAWGRNGNIDINNLNNFRTRSSILIEINN
                     TNILIDTSPDLRQQLYSAKCTNIDAVLFTHIHTDHTSGLPDMRAISLINDKIIPAYMS
                     KDMKDKILSHYKFIFKGDKDYRPFMEVRDIQNKFFINNIEIQTFKHNHGSIDVLTYRI
                     GKFAYSTDLKNFYENDLDNLQDLDLWVVGLLRSDPHPSHAGFEQILDYIKYIKPKKTI
                     FTHMTALLDEEKLISKCPKNVLPAFDGMEIEI"
     gene            1905..2852
                     /locus_tag="EYO86_01885"
     CDS             1905..2852
                     /locus_tag="EYO86_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC41818.1"
                     /translation="MKDVNVGFIGLGNVGSKIARNILKGGFKLFVNDLDRNLSRNWLE
                     VGAQWCFDLKNLASQSTIIITCLPSPVAVNNVLVKILPFLNTNHLWIEMSTTDESEMI
                     RLSKLCLQKGIESLEAPITGGQHRAEKGNISILVAGKRKNFERAFPLLSEIGYEILYC
                     GKLGNASTLKVVTNYLASINLLSLGEALMVCKKYGLDLTTAYHGIRISSGNSFVHETE
                     SKVILNGSYDVGFTMDLVCKDVGLFDKLSDKYNIPADISKLINKIFNEGRGKYGDRSF
                     STSIVKRLEEACNENLRAPGFPSQLKDEEPKKKAIEIKF"
     gene            2859..>3086
                     /locus_tag="EYO86_01890"
     CDS             2859..>3086
                     /locus_tag="EYO86_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41819.1"
                     /translation="MNKKKDFSPTVYKFKDAVMEQVENTDLFKSYIKTTEFKQLFSGT
                     LWAEGPCYIPHKDMLVWSDNPNNRMMKLVKGQ"
BASE COUNT         1165 a          395 c          469 g         1057 t
ORIGIN      
        1 ttgaattagt tagtcagata aataagaaaa attttaattc aataattggt ttaatagaaa
       61 aaacaattaa attaaaaaga aatatttgaa ctgttaacag aaacaaatgt atatgttgat
      121 caacaagcgc cttgggtttt aaacaaatca aatatagcta gaatgaatgt agttttatct
      181 ttaactgttg agctaataaa aagatcaaca ctaatgctat atcctgtaat tccaggtagt
      241 tgtttaaaag tttttgaaat attgaatcta aatttttctt ctattaattt tgataatata
      301 gataatttac cctctacttc attaactatt aatgaaccaa gtccaatttt tccgcgtata
      361 gtaattgatg attgattcac attgtcatct caattttgac aaccttgcta ttgaaattga
      421 gaatctcatt cgtaaagcaa aaaataataa tattacttcc attctttcta taaatactga
      481 tcctgatgaa ttcgacaacc attacaaatt aataaaaaaa tttaaatcta tctttcttag
      541 ttacggttta catcctcaac aagtaactga aagtaacatc attaattcgg ctaagatcat
      601 taataaaagc tctaatgatc gagtaattgc aataggggag acaggtttag atttttttca
      661 ttcagtcaaa tacaaaaaga agcaatatga agtttttgaa agtcatatag aggcatcatt
      721 tgaaacagat cttccactta ttatacatca aagaaattcg gaaaacgaaa ttatggatgt
      781 tttaagtaat tttcaaaaat caaaatctct taaagttgtg tttcattgtt ttaccggttc
      841 ttcaaaacta cgtaattttt gcttggaaaa tggtttttat ctatcgttat ctggaataat
      901 tacatttaaa aatgcagaag agctaaggag tgttataaaa aatgtaccat tatcttctgt
      961 attaattgaa acagacagtc cttttttaac tccaactccc caccgtggta aaattaatga
     1021 accttctttt gtaaaacatg ttgctgaata tttatcaata ttttttaaag taccctttga
     1081 agaatttata tctattactg ataataattt ttataaattg ttttctaaag caattcgata
     1141 taatgaaatt tcaatatgaa aataagagta cttggtagtg gtagctcatt tggttcgcct
     1201 ttagcttggg gccgcaacgg taatatagac attaataatt taaacaattt tcgaacacga
     1261 tcttccattt taattgaaat taataataca aatatattaa ttgacactag tcctgaccta
     1321 aggcaacaat tgtacagtgc caagtgcaca aatattgatg ctgttttatt tactcatatt
     1381 cacacagatc atacatcagg ccttcccgat atgagagcta tttcattaat taatgataaa
     1441 ataataccag cttatatgtc taaggacatg aaggataaaa ttttatctca ctataaattt
     1501 atctttaaag gagataaaga ttacagacca tttatggaag taagagatat tcaaaataaa
     1561 ttttttataa ataatattga aatacaaacc tttaaacata accatggttc catagatgta
     1621 ctaacttata ggataggaaa atttgcttat tctactgatc taaagaattt ttatgaaaat
     1681 gatttagata atcttcaaga tttagattta tgggtagtag gtcttttaag aagtgatcca
     1741 catccttctc atgctggatt tgagcaaatt ttggattata ttaaatatat aaaaccaaaa
     1801 aaaaccatct ttactcatat gactgcatta ttagatgaag agaaattaat ttcaaaatgt
     1861 cctaaaaatg tattacctgc ctttgatgga atggagatag aaatatgaaa gatgttaatg
     1921 ttggttttat tggtttagga aatgtaggat caaaaatagc tagaaacata ttaaaaggcg
     1981 gctttaaatt gtttgttaat gatttagatc gtaacttatc aagaaattgg ctagaagtag
     2041 gtgcgcaatg gtgttttgat cttaaaaatc tagcatccca atccacaatt attataactt
     2101 gcttaccttc tcccgttgca gttaataatg ttttagtaaa aattttacct tttctaaata
     2161 ctaatcatct ttggatagaa atgagcacaa cagacgaatc agaaatgata cgtttatcaa
     2221 aattatgttt acaaaaaggc atcgaatcat tggaagctcc tattactgga ggacaacata
     2281 gagctgaaaa aggaaatata tcaatcttgg tagcgggtaa aagaaaaaat tttgaaagag
     2341 ctttcccatt actatctgag attggttatg aaattttata ctgtgggaaa ttaggaaatg
     2401 catctacact taaagtagta acaaattatc tggcatcaat taatttactt tctttaggag
     2461 aggctttaat ggtttgtaaa aaatatggtt tagatttgac aacagcatat cacggtattc
     2521 gtattagttc tgggaatagt tttgttcacg aaactgaatc taaagttata ttaaatggct
     2581 cttatgatgt tggttttaca atggatttag tatgtaaaga tgtaggatta tttgataaat
     2641 tatcagacaa atataatata cctgcagata tcagtaaatt aataaataaa atattcaatg
     2701 aaggaagagg taaatatgga gatagatctt ttagcactag tattgttaaa agattagaag
     2761 aggcttgtaa tgaaaattta agagctcctg gttttccaag ccaattaaaa gatgaagaac
     2821 ctaaaaaaaa agcaatagaa attaagtttt gattaaatat gaataaaaaa aaagatttta
     2881 gtcccacagt ttataaattt aaagatgcag taatggagca agtagaaaat acagatttat
     2941 ttaaaagtta tattaaaact actgagttta aacaattatt tagtggcact ctatgggctg
     3001 aaggtccttg ttatatacct cacaaggata tgttagtttg gagtgataac cccaataata
     3061 gaatgatgaa acttgttaaa ggtcaa
//
LOCUS       DTVV01000160            3927 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1402122, whole genome shotgun sequence.
ACCESSION   DTVV01000160 DTVV01000000
VERSION     DTVV01000160.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1402122"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(66..3407)
                     /locus_tag="EYO86_01895"
     CDS             complement(66..3407)
                     /locus_tag="EYO86_01895"
                     /inference="COORDINATES: protein motif:HMM:PF05729.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HIC41820.1"
                     /translation="MNQEYKLYRVSQLLSRFSEQVKILNSNSEFGINIHAENLLIKLL
                     NVIFECDLKNVNYEEGKNYPSIDLRDPKNRIAIQVTSTSSLDKIKSSLEKFVQNRLYE
                     DFDKIIFFIITEKQSTYDLTVTKRIINGKFDFSTNNILDKRDLYLELNRQNDINKIDE
                     FSQILESQFTDNFDELNKWNIYCEGLNSYDKFIQNKHHYLDVKGFSPKINNTLVKLEL
                     DKVFVPLKLSTSLNSEAPSFSEAMENYYIEKALDRFKKIVILGDPGSGKSTLLKKLAF
                     NICSKRELFDSVPVIVKGSEFAKYVSDTNRGLAEYLIDHIDKKYAELFASKLHDNKLL
                     VLLDGIDEINQHSLKHSVSERVNAFISQYPDIQIIVSSRVVGYQENCLNGYFTHLTVS
                     KFEKEQIKTFIENWYQALSRNTSQTVDESQELYNSICKNQSVLKMASNPLLVTIIALI
                     HYQGSTLPERRATLYDIATSTLLDNWVKQRNTGKKSIIDKDTLIEILAPVAFTMHENS
                     TTGLICERNLKSKLTEEFKLISSFTNDFNIKKDVKDIIEFLREDAGFLFEKGVDENGE
                     SMFGFVHQTFQEYFTAIELKTKWKEGGLQESLKSLVLSSNWHEVIKLTASLFKHSEQS
                     RVGRKDTTNFINQIFLIEDDLIETQRPLSLVVNILGEDTEVERQLFNNIFEAIINCGR
                     QALKNKSNTNISYLLEILLNTQIYKNYVLQKMEALIYDENDNDEQLKYELISTLMSSS
                     NQREVKESLDRVLNGNDIKLKEHMFDNRIVFPISPIVTTELFNSKIVEYVNSDYFNNK
                     KEKRIPTQYTICYEDWRESPLDAAFASIETIKEFEAKLLSRYMAAIENLKDNELKQQL
                     IDDYYDTVSFFSLETLQDYLSQLKQKFPSMSTSVIEKRINFYEEKEKLCLGDDCLVSH
                     QGIRIYPCIDSKDSFAFIKGSVITKLKYPFKKSEIVFEDIADSDSFLEYLNFFLLSYT
                     DPTKDLTIDSKETILLFIKFDDIYDWKRQEQLSPIRAKAFDFLFCDDNLIEKNISNWL
                     IDMYIKHPMISRAIPQNEIHNVDVLIKKIKKSEMPKHEKLCLLLMLGDEHEKENLIKE
                     SIIEYQTRTSAAEKDRLHKILAKVM"
BASE COUNT         1223 a          638 c          590 g         1476 t
ORIGIN      
        1 agccagattg tcctcaaaaa tgagaaagta ccttatctaa ctgaaatgtc cattacttac
       61 aatatttaca taactttagc tagaatttta tgtaatctat ctttttctgc agcagaagtt
      121 cttgtctgat attctataat actttcttta ataagatttt ctttttcatg ttcatcacca
      181 agcattaaaa gtagacataa cttttcatgt ttaggcatct cacttttctt gattttcttt
      241 atgagaacat ctacgttatg aatctcattt tgaggaattg ctctggatat cataggatgc
      301 ttaatgtaca tatctatcaa ccaattagag atattttttt ctattaagtt atcgtcacaa
      361 aataaaaaat caaatgcttt agctcttatt ggagatagct gttcttgcct tttccaatca
      421 tagatgtcat caaatttaat aaatagcaaa atagtctctt tagagtcaat agttaaatcc
      481 tttgtagggt cggtgtaact taataagaag aaatttaaat actctaaaaa tgaatctgag
      541 tcagcaatat cttcaaagac tatttcactt tttttaaatg gatattttag ttttgttatt
      601 actgaacctt taatgaatgc aaaggagtct tttgaatcaa tacaagggta tatacgtata
      661 ccttggtgac tgacaagaca atcatcacct agacaaagtt tctctttttc ttcgtaaaaa
      721 ttaattcttt tttctattac agatgtgctc atagaaggaa atttttgttt tagttgactc
      781 aaataatctt gtagagtctc aagactaaaa aaacttacag tgtcataata gtcatctatt
      841 aattgttgct tgagttcatt gtctttaagg ttttcaatag cagccatata acggctcaac
      901 aactttgctt caaattcttt tatggtttca attgaagcaa atgcagcgtc taggggactt
      961 tcgcgccagt cctcataaca aattgtatac tgagttggaa ttcttttttc tttcttgtta
     1021 ttaaaataat cagaattaac atattctaca atttttgaat taaacaactc tgtagttacg
     1081 ataggggaaa tagggaaaac aatcctattg tcaaacatat gctctttcag ctttatgtca
     1141 ttgccattca aaactctatc taatgactct ttaacctctc gttggtttga actcgacatt
     1201 aaggttgaaa ttaattcata tttcaattgc tcatcattat cattttcatc ataaatgaga
     1261 gcttccattt tctgcaatac atagttttta tagatttgtg tgttaagtag tatttctaac
     1321 aagtagctaa tattagtatt gcttttattt ttaagagcct gacgcccgca gttaattatt
     1381 gcttcaaata tgttattaaa aagctggcgt tcgacttccg tatcttcccc tagaatattt
     1441 acaacaagag acagggggcg ttgcgtttca attaaatcat cttcaattag aaaaatttgg
     1501 ttgataaaat ttgtggtatc ttttcttcct actctagatt gctcactgtg tttgaaaagt
     1561 gatgctgtaa gctttattac ttcatgccaa ttagagctca acacaagtga tttcaaagat
     1621 tcctgaagtc caccttcttt ccatttggtt tttaattcta ttgcggtgaa atactcttga
     1681 aaagtttggt ggacgaaccc aaacatcgac tcaccgtttt catcaactcc cttttcaaat
     1741 aaaaagcctg catcttctct tagaaactca ataatatctt ttacatcttt ttttatatta
     1801 aaatcattgg taaacgagct tatcaattta aattcttcag ttaacttgct ctttaagttt
     1861 ctttcgcaaa ttaaccctgt cgtactattt tcatgcatag taaacgcaac tggagcaagt
     1921 atctcaatca aagtgtcctt atctattatt gatttctttc ctgtgttgcg ttgtttaacc
     1981 cagttatcca aaagtgtcga tgtggctatg tcgtatagtg tcgcccttcg ctctggcaaa
     2041 gtggaaccct gataatgaat taatgcaatt attgttacta ataagggatt actagccatt
     2101 ttaaggacgg attgattttt acaaatagag ttataaagct cttgagattc atctacagtt
     2161 tgacttgtat ttctagagag tgcttgatac cagttttcaa taaatgtctt tatttgctct
     2221 ttttcaaatt ttgacactgt caggtgtgta aaatagccat ttaaacaatt ttcttgatac
     2281 ccaaccactc tagaagaaac tataatttgt atgtctggat attgcgatat aaatgcatta
     2341 actcgctcag aaacactatg ttttaggcta tgttggttga tctcatcaat accatcaagt
     2401 agtacaagta gcttattgtc atgtaatttt gacgcgaata attctgcata ctttttatct
     2461 atatgatcta ttaaatattc agcaagcccc cgattagtat cagatacata tttagcaaat
     2521 tcactccctt taactatcac aggaaccgag tcaaacaact ctcttttact acatatatta
     2581 aaagctagtt tcttcaaaag tgttgacttt cctgaaccag gatcaccaag aatgactatt
     2641 tttttaaagc ggtctaatgc tttctctata tagtagtttt ccatcgcttc actgaaggag
     2701 ggcgcttcag aattcaaact agttgatagt tttaatggga cgaacacctt gtctagctct
     2761 aatttaacaa gcgtattgtt tattttgggt gaaaaaccct ttacatcaag gtagtgatgt
     2821 ttattttgaa taaatttatc gtatgaattg agtccttcac agtatatatt ccacttattt
     2881 agttcatcaa aattatctgt gaattgactt tccaatattt gtgaaaattc atctatttta
     2941 tttatatcgt tttgacgatt taactcgagg tataaatccc ttttgtccaa aatgttattc
     3001 gttgaaaaat caaatttacc atttatgatt cttttagtta cagttaggtc atatgttgat
     3061 tgcttctctg tgattataaa aaaaataatt ttatcaaagt cttcatatag tcgattctgg
     3121 acgaattttt ctaaagatga tttaatttta tctaaagatg atgtagaggt tacttgaatt
     3181 gcaattctat ttttaggatc tctcaagtct atacttggat aattcttacc ttcttcatag
     3241 tttacgtttt ttaaatcgca ttcaaaaatt acattaagta acttaattaa taggttttcc
     3301 gcatgaatat tgattccgaa ctcagagttt gaattgagta tctttacttg ttcgctaaat
     3361 ctagatagta attgcgatac tcgataaagc ttatattctt ggttcattcc attgcctgta
     3421 tattaatcat gagttattgt tcaaaaatac catgaaaatt tatcgatagt acagttattt
     3481 acaggtattt aagtaaaagc actttcttaa cttagttaag ggcttttcaa gagcaaagtt
     3541 tcaagtttgg tactttgtag taataagaat agggggagtg gttattagaa cactgaacac
     3601 taattttagc gattcttttt atcagatgag tttggctttt tcaacttggt tatcgcttat
     3661 aataatagcc ctagtttcca tggtaggttc ttgtaaaatg gcaatgttga taaatattaa
     3721 cctttttcgc ttagggtaaa tgagtcactc atctttacag cagttatgtt agcttgtttg
     3781 ggtaagaaag aattaaaccg gaaggggagt ttatagcaca atgtctactt ttacttcaaa
     3841 atcaaaaata ctcaatatag cgttagaaaa tttactgtgc tcacacgtta aagctaacaa
     3901 aaccagtaag aataaaaaaa gagtaaa
//
LOCUS       DTVV01000161            2159 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1405004, whole genome shotgun sequence.
ACCESSION   DTVV01000161 DTVV01000000
VERSION     DTVV01000161.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1405004"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="EYO86_01900"
     CDS             complement(<1..684)
                     /locus_tag="EYO86_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HIC41821.1"
                     /translation="MKAVALNISAWMLLPVMDGMAKHLSAEIHFLQVVWGRYFFMVII
                     SLPLTYFFFYNHLKFPKNIWIQLIRSSFLFLSTVFFFFSISIISLAEALSLMFVAPII
                     VTLLSALILKEQVGIRRWCAVIFGFIGALIIIRPGFEEINIASIAALGAGLSYGFYII
                     STRKLSFIDSPLLTLIFTGLSGAIFVSFIVPFFWTSLDPIQWLLLIALAAVGTLGHLL
                     LILSLNFAEA"
     gene            complement(901..1116)
                     /locus_tag="EYO86_01905"
     CDS             complement(901..1116)
                     /locus_tag="EYO86_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41822.1"
                     /translation="MATVTSWTETLPAGETVIYPFVGTEWLWLLIAVVFWLTWHFKTS
                     AGETEEHNELAAKGKGPSDYKNNIADW"
     gene            complement(1129..>2159)
                     /locus_tag="EYO86_01910"
     CDS             complement(1129..>2159)
                     /locus_tag="EYO86_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIC41823.1"
                     /translation="IMGVFWAAFLLFSWTAASIVSGAVIERIRISAFGILAIAIGSVF
                     WSIDASWGWHFDGWMVKILGYHDAYASGVIHAVCGGFALGVLAVLGPRIGKFASDGTP
                     RNIGPRNPWLVAIGLFLIYTGFWGFYAACNIPIFDLGPEYGMEGVTYFTATNIYLTPT
                     TLSGITMNFLLSLSGGLLAGYWISKGDPFWTYSSGLAGIITSSAGNDLYHPLQAMIIA
                     AIGVYVAYKMHFWVERRFKIDDAVGAVAVHGYAGVVGLVICGFVLWGYPSSGYEGYAA
                     INPIGMIIGAIIMFGVLGFLPGWIIAKILHSMGKLRIPHEVELAGMDFNYMEASKADE
                     TALAADEK"
BASE COUNT          784 a          450 c          349 g          576 t
ORIGIN      
        1 agcttcggca aaattcagtg ataagattaa cagtaagtga ccaagggttc ctacagcagc
       61 taaagctatt agtaataacc attgaatggg atcaagcgaa gtccaaaaaa aaggaacaat
      121 gaatgaaaca aagatagctc ctgataaacc tgtaaatatt aaagttaaaa gagggctatc
      181 aataaaagat aattttcttg tgcttataat gtagaaaccg taacttaatc ctgcacccaa
      241 agctgcaata gatgctatat taatttcctc aaatccagga cgaattatta ttagagctcc
      301 tataaatcca aatattactg cacaccatct tctaatacct acttgttcct taagaattag
      361 agctgagagc aaagtaacaa taattggcgc aacaaacatc aaggataatg cttctgccaa
      421 tgatattatc gaaattgaaa aaaagaaaaa aactgttgat aaaaaaagaa aagaacttct
      481 tattaattga atccaaatat ttttaggaaa ctttagatgg ttataaaaaa aaaaataagt
      541 taaaggtagg cttattataa ccataaaaaa atacctgccc caaacaacct ggagaaaatg
      601 aatttcagca ctgagatgct tggccatgcc atccatgacg ggtaacagca tccatgcgga
      661 tatgttgaga gctacggctt tcataaaaaa ttatttaaag aatataagta aggaacaaat
      721 aatgattatg acaacagcaa tatacaaacc gtattttgct ggaggaaaag caacattgat
      781 catcctatat ggccttttct tcttgttgtc attttcagaa ttcatttgta tttgatgagt
      841 ataaaaaaag ggcgtctttt acaacgccct atttattatt ttttatgaga tatgttaata
      901 ttaccaatca gcaatattat ttttataatc actgggacct ttgcctttag cagccaattc
      961 attatgctct tcggtctccc cggcagatgt tttaaaatgc caagttaacc aaaaaacaac
     1021 agctattaac agccatagcc attcagtacc tacaaaagga taaataacag tttcccctgc
     1081 aggaagcgtt tcagtccaac tagttacagt tgccataaat taccctcctt atttttcgtc
     1141 ggcagctaga gcagtttcat ccgctttaga tgcttccata tagttgaagt ccatacccgc
     1201 taactctact tcatgaggta ttctaagttt acccattgaa tgtaaaattt tagcaataat
     1261 ccaaccaggt aaaaatccta ggacaccaaa cattattatc gcgccgataa tcattccaat
     1321 tggatttata gccgcataac cttcgtaacc tgaagatggg tatccccaca aaacaaagcc
     1381 acaaattacg agtccgacca cgccagcata accatgcacg gccaccgcgc ctacagcgtc
     1441 gtcaatttta aatcgacgct caacccaaaa gtgcattttg tatgcaacat acacaccaat
     1501 agcagcgata atcatagctt gtaatgggtg gtataaatca tttccagcag atgatgtaat
     1561 aattcctgcc agaccacttg aatatgtcca aaaaggatct cctttagaaa tccagtaacc
     1621 tgccaacaaa ccacctgata gtgatagtag gaaattcatc gtaataccac ttagggttgt
     1681 aggagttaaa taaatattag ttgcagtgaa atacgttaca ccttccatgc catattcagg
     1741 tccaagatca aaaattggaa tattacaagc tgcgtagaaa ccccaaaaac cagtataaat
     1801 taggaataat cctatagcaa ccagccaagg atttcttggt ccaatatttc ttggtgttcc
     1861 atcagaggca aatttaccaa tacgaggacc aagaactgcc agcacaccaa gggcaaatcc
     1921 accgcaaacg gcgtgaatta ctcctgaagc gtaagcatca tgataaccta gtattttaac
     1981 catccaacca tcaaagtgcc atccccaaga tgcgtcaata ctccagaata cagagccgat
     2041 agctatagct aatattccaa atgcggatat tctaattctc tcaataacag cacctgaaac
     2101 tatagaagct gctgtccaag aaaatagcaa gaacgcagcc cagaaaactc ccatgatat
//
LOCUS       DTVV01000162            1095 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1405378, whole genome shotgun sequence.
ACCESSION   DTVV01000162 DTVV01000000
VERSION     DTVV01000162.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1405378"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            185..>1095
                     /locus_tag="EYO86_01915"
     CDS             185..>1095
                     /locus_tag="EYO86_01915"
                     /inference="COORDINATES: protein motif:HMM:PF01381.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HIC41824.1"
                     /translation="MIGEKKRKINITDLAKKLNLSVSSVSRALNGHKNISEKTKAKIL
                     KLAKKYNYSPDLSAKRLASKKSDTIAFIATISPYAPDYVLLEFLAGITLGIKDSSTEL
                     LIKFSLNEKEEINYYQDLINSNQADKFIFYRTKQKDLRIELLQKNKINFLSWGRSKSY
                     KDYAWIDMDNHKSIDLLMSRLLIFGHKKIALINVHQSFNYGYQRKQAYEKMLKKNNMI
                     VNKDYYQESLTATAANGISLTKFLLDLKDPPTAIICSQDKFFIGCLQECRNRNLVIGS
                     NISVVGYNDYDNYLSSQNLTFISHPLS"
BASE COUNT          411 a          177 c          160 g          347 t
ORIGIN      
        1 gcacgagaaa tattgatggt catttctatt tcatccccta attcaaagct tgcactccct
       61 tcaattctga ataaatattt tgtattattt cttagtaaaa cgttttttta gtaaaacgtt
      121 ttacttgata gttgtcaagt aatagatatg atgacattta gaattaattg ggacaattac
      181 gaaaatgatt ggggaaaaaa agagaaaaat taacattaca gatctagcaa aaaagctcaa
      241 tctgtcagta agttcagtct ccagagccct aaatgggcat aaaaacatat ctgaaaaaac
      301 taaggccaaa atactaaaat tagcaaaaaa atataattac tctcctgact tatctgccaa
      361 acgattagcg tctaaaaaat ctgataccat agcctttata gcaactatca gtccttacgc
      421 ccctgattat gttctcttag aattcctggc gggtataact ttgggaatta aggatagctc
      481 aactgaatta cttataaaat tttctttgaa tgaaaaagag gaaattaatt attatcaaga
      541 cttaatcaat tccaatcaag ctgataaatt tattttttac cgcaccaaac aaaaagatct
      601 ccgtattgaa ttactgcaaa aaaataaaat taattttttg tcttggggaa gatccaaaag
      661 ttacaaagat tatgcctgga ttgatatgga caatcataaa agtatagatt tgctgatgag
      721 cagattgtta atatttggtc acaaaaaaat agcattaatt aacgtgcacc aaagttttaa
      781 ttatggctac caaagaaaac aagcctatga aaaaatgtta aaaaaaaaca atatgattgt
      841 taataaagat tattaccaag aatctttaac cgccactgcc gctaacggta tttctctaac
      901 aaaatttctt ttggatttaa aagatccccc aactgcaatt atatgctcac aggataaatt
      961 ttttattggg tgcctgcagg aatgtagaaa ccgaaacctt gtcattggaa gtaatatttc
     1021 cgttgttgga tacaatgatt atgacaatta tttaagttca caaaatctga cttttattag
     1081 tcatccactt tccca
//
LOCUS       DTVV01000163            2666 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1410753, whole genome shotgun sequence.
ACCESSION   DTVV01000163 DTVV01000000
VERSION     DTVV01000163.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1410753"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="EYO86_01920"
     CDS             <1..849
                     /locus_tag="EYO86_01920"
                     /inference="COORDINATES: protein motif:HMM:PF02353.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC41825.1"
                     /translation="QSKNNVAHHYDLNEDLYRLMLDKDMQYSCAYFHNENISLEQAQT
                     DKKKHIIKKLQIKDDMEVLDIGCGWGGMALQIVKDTGARVKGITLSENQLATAQRRAQ
                     TEGLNEKVKFSLQDYRHEKYKYDRIISVGMFEHVGASYFPEFFSKTYDLLKDPGIFLL
                     HTIGKRGKPVATSPWIRKYIFPGGYIPTLSEITKITEKYNMNITDIEILRMHYAHTLS
                     HWYKRTMDHKKEIIKMFDERFLRMWEFYLLISKYSFVNMGNVVYQIQIAKNIHNLPLT
                     RNYIYN"
     gene            926..1969
                     /locus_tag="EYO86_01925"
     CDS             926..1969
                     /locus_tag="EYO86_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41826.1"
                     /translation="MQGSLNPYLIRLSVFILLILIVVVFLYPLLQTAFLSNIYINLII
                     ILALVFGLIFNVYILSRLNSDYSSLANFNIHKSPQVFLASSSLLKGLSQQIHEKDGRY
                     TFKSSKIEKILESVDMSLLSLRETSRYLVGLLVFLGLLGTFWGLLKTIGSVGNVISGL
                     GIDDTNVAGFFNSLKDGLHAPLQGMSVAFSSSLLGLAGSLILGFADLNLGQAQNKFTQ
                     FLEKILQNNSSPDLINTSPSDPSTLSAIQKIYDNLDNLVYTLKETSHHLSQIFSYMQS
                     LTEQMKGLNVNSREQDKKLSNFLSTQLNSQSNMLQLTSQLSQEGLIDKQTKKYFENID
                     KGIQQLISNSKKK"
     gene            1973..>2666
                     /locus_tag="EYO86_01930"
     CDS             1973..>2666
                     /locus_tag="EYO86_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41827.1"
                     /translation="MSARSRRYRLNETNNIWPGFVDVLATLLIVIIFVLMIFTVSQIY
                     LSDAISGRDKALENLRSQINELSKILVIEKKEKQQVLTSLEQTQDQLAQTESDLRTQE
                     MYSQGLQSDIAKKESELFIQDQNILALSDQIKVLLVELRIVAKALETYEGAEIASLNT
                     EGLGERINKALATRIDQLNQLNEDLDEVNIALAQSETNLQNKINDLDIANTELALNKT
                     ELENKINDLDIAN"
BASE COUNT          949 a          404 c          462 g          851 t
ORIGIN      
        1 caatcaaaaa ataatgtggc acaccattat gatttgaatg aagatttgta ccgcttaatg
       61 ttagataagg atatgcagta ttcttgtgca tattttcata atgaaaatat tagtctagaa
      121 caggcgcaaa ctgataaaaa aaaacatatt attaaaaaac tacaaatcaa ggatgatatg
      181 gaggttttag atatcggatg tggttggggt ggcatggctt tacagattgt aaaagatacg
      241 ggggcaaggg taaaaggaat tacactatca gaaaatcaac ttgctactgc tcaacgaaga
      301 gcacaaacag aaggcttaaa tgaaaaagta aaattttcat tacaagatta ccgacatgaa
      361 aaatataagt acgatcgtat tatatccgtg ggcatgtttg agcatgttgg tgcaagttat
      421 ttcccagaat ttttttctaa aacttatgac ttactaaaag atccaggcat atttcttttg
      481 catactattg gtaagcgagg gaaacccgtt gctaccagtc catggatacg taaatatatt
      541 ttccctggcg gttatatacc caccttatct gaaattacta aaataacaga aaaatataat
      601 atgaacatta ctgatataga aatattgcgt atgcactatg ctcatacatt gagtcattgg
      661 tacaaacgta ctatggatca caaaaaggaa attatcaaaa tgtttgatga gcgatttctg
      721 cggatgtggg aattttattt attaattagc aaatattctt ttgtgaatat gggaaatgtt
      781 gtttatcaaa tccaaatagc aaaaaacatc cataatttgc cacttacacg caattatata
      841 tataattaaa atagcttttt gtctatatct gccttaattt acaaatgatt ttagtatacc
      901 aacacattcg ataggagaga acattatgca gggatcactt aacccatatc taataagact
      961 aagtgttttc atattattaa ttttaattgt agtcgttttt ctctatcctt tattacagac
     1021 agctttttta tcaaatattt atattaattt aatcattatt ttagccttgg tgtttggttt
     1081 aatttttaat gtgtacatct tatctaggtt aaatagtgat tacagttctc ttgctaattt
     1141 taatattcat aaatcgccac aagtatttct tgcctcatct tcgttgctta aaggtttatc
     1201 acaacaaata catgaaaaag atgggcggta tacatttaaa agttcaaaaa tagaaaaaat
     1261 tttagaatca gttgacatga gtcttttgag tttgcgtgaa acatcccgct atctcgtggg
     1321 cctgcttgtt ttcctaggtc ttcttggaac attttgggga ttgttaaaga caattggttc
     1381 agttggaaat gtgattagtg gcttgggaat agatgatacc aacgtcgcag gattttttaa
     1441 tagtcttaaa gatgggttac atgcgcccct tcagggaatg agcgttgcct ttagtagttc
     1501 tttgttgggt ttagccggtt ctttaatttt aggatttgca gatcttaatc tgggacaggc
     1561 gcaaaataaa ttcactcagt ttttggaaaa aattctacaa aataatagct ctcctgactt
     1621 gattaataca agtccatctg atccatctac cttgtctgct attcaaaaaa tttatgataa
     1681 tttagataac ctcgtctaca cgttaaaaga aacttcccat cacctaagtc aaattttttc
     1741 atatatgcaa tctttgactg aacagatgaa aggattgaac gttaattcta gggaacaaga
     1801 taaaaaatta tcgaactttt tatcaactca attaaattcg cagtcaaata tgttgcagtt
     1861 aacaagtcaa cttagtcaag aaggcttaat agataaacaa acaaaaaaat attttgaaaa
     1921 tattgacaaa ggtattcaac aactcatttc taattcaaaa aagaaataac ttatgtcagc
     1981 aagatcgaga cggtatcgac ttaatgaaac gaataatatt tggccaggct ttgttgatgt
     2041 tttagctaca cttttaattg ttattatatt tgtgttgatg atatttactg tatcacaaat
     2101 atatttaagc gatgccatat cagggcgaga taaagctctt gaaaatttac gtagtcaaat
     2161 taacgaactc tctaaaatac ttgttataga gaaaaaagaa aaacaacagg ttttaacctc
     2221 cttggaacaa acacaagatc agttagctca aacagagtct gatttgagaa cacaagagat
     2281 gtattctcaa ggtttacagt ccgatattgc aaagaaggaa tcagaattat tcattcagga
     2341 ccaaaatata ttagctttga gtgatcaaat aaaagtatta cttgttgagc taagaattgt
     2401 tgcaaaagcc cttgaaacat atgagggtgc agagattgct tcgttgaata ccgagggttt
     2461 gggggagaga attaacaaag ctttagcaac tagaattgat caactcaatc aattaaatga
     2521 agatctggat gaagtaaata tcgcgctagc acaaagtgaa acaaatctgc aaaacaaaat
     2581 aaacgacctg gatattgcta acaccgaatt ggcgcttaat aaaacggagc tggaaaacaa
     2641 aataaacgac ctggatatcg ctaaca
//
LOCUS       DTVV01000164            1309 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1414336, whole genome shotgun sequence.
ACCESSION   DTVV01000164 DTVV01000000
VERSION     DTVV01000164.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1414336"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(203..1054)
                     /locus_tag="EYO86_01935"
     CDS             complement(203..1054)
                     /locus_tag="EYO86_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HIC41828.1"
                     /translation="MYRTLLYITLLFFFSITFSVKAERAYCAKYEECREARWTDLKKT
                     LFEDIDLKSGEGIIELTTPERAPDAAFVPISFKTHKLKNQDDFITKVHLVIDMNPSPY
                     SGTFKFATNIDQIELATRIRLDAYSKVRVIAETKKGDYFMAANFVMGSGGCSAPPLGD
                     LDNMIASAGKMKIKVKNIEEKDGIAEAKIMILHPQFNGLQANPATTEMIPAYYIENIK
                     VTYNDKVVMALESDISISQDPTFRFNFLMGDGGDLKVTAKDTDGNIYSHSEKLALQDN
                     QLKIASQ"
     gene            complement(1113..>1309)
                     /locus_tag="EYO86_01940"
     CDS             complement(1113..>1309)
                     /locus_tag="EYO86_01940"
                     /inference="COORDINATES: protein motif:HMM:PF13599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIC41829.1"
                     /translation="SRTDLEYASFKNANLSNANLEFAKLTGVNFVGANLTNTNFTNAE
                     LGANHFCGSIGLDKAIGLKK"
BASE COUNT          433 a          214 c          170 g          492 t
ORIGIN      
        1 aataaattaa ggtcaactaa tgcaatattc attattcata aaatcaataa tagtcatatt
       61 aataataaat tacgttaata gtatataagg tctatattat gagttgtaaa gaacaaaaag
      121 tttatcctcc tctatctccc tatgaaaaaa aaagccctta tctcagggct tttttcttat
      181 atatcttttt tattatttat tattactgag aagctatttt taattgatta tcttgtaatg
      241 caagtttttc agagtgtgaa tatatattac catcagtatc tttagctgta acctttaaat
      301 cacctccatc acccattaga aaattaaatc taaaagtagg atcttggctt atggaaatat
      361 cagattctaa ggccataact actttgtcat tataagtaac tttaatattt tctatataat
      421 aagctgggat catttcagta gttgctggat tcgcttgtaa accgttaaat tgcgggtgta
      481 atatcattat ttttgcttct gctatgccat ctttttcttc aatgtttttt acttttattt
      541 tcattttacc tgcgcttgca atcatattgt ccagatcacc taatggagga gcagaacatc
      601 cacctgaacc cataacaaag tttgcagcca taaaataatc tccttttttt gtctctgcta
      661 taactcttac tttggaatat gcatccaatc taatccttgt tgccaattca atttgatcta
      721 tgttagtagc aaatttaaaa gttccagaat aaggagatgg attcatatca ataactaaat
      781 gtactttagt aatgaaatca tcttgatttt taagtttatg tgttttaaaa cttattggaa
      841 caaatgcagc gtcaggtgcg cgttccggag tagttaattc aattatgcct tctccacttt
      901 taagatctat atcttcaaaa agtgtttttt ttaaatctgt ccatcttgct tccctgcatt
      961 cttcatattt agcacaatat gctctttctg cttttacaga aaatgtaatt gaaaagaaga
     1021 acagtaaagt aatatataat aatgtgcgat acatattttt acattatata ctctataaat
     1081 tataaattac aatataacga tcatcttgga aactattttt ttaaccctat agctttatca
     1141 agaccaatag aaccacaaaa atgatttgca ccaagttcgg cattagtgaa attagtattg
     1201 gttagattgg ctccaacaaa attaactccc gttaactttg caaattctaa attagcatta
     1261 gaaagattgg catttttaaa tgatgcatat tccaaatcag ttcttgaca
//
LOCUS       DTVV01000165            1829 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1417404, whole genome shotgun sequence.
ACCESSION   DTVV01000165 DTVV01000000
VERSION     DTVV01000165.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1417404"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            97..1608
                     /locus_tag="EYO86_01945"
                     /pseudo
     CDS             97..1608
                     /locus_tag="EYO86_01945"
                     /inference="COORDINATES: protein motif:HMM:PF09972.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
BASE COUNT          635 a          259 c          372 g          563 t
ORIGIN      
        1 acatttacta tgctggaata gttctttata gtattcagac taatcatttt ttgcagtcca
       61 aaaaactaat attattaaaa tagggaaata tgctcaatga gattaaatag aacctcattt
      121 attttattat gtacatccct gattttcggg aaatcatata cgattgataa tgtcaaaatt
      181 gaatcaacaa tcgggggaga tgggatcgtt caaataaccg aaaaaaggac ctttaatttt
      241 cgaggtagat ttagttatgc atatcaaaaa attgataaaa aactatttaa ggaaatatat
      301 gatatccaat tatcagaaaa tggttaccag tatataaata acagttcatc ggaaccgtat
      361 acatttactg ttacctcaaa taagaaaagt attaagatta aatggtttca tgaatcggaa
      421 gatatttcta aggagtttgt tgtatcctat aagttgcgag gtgcgcttcg aattggacca
      481 gacgacgctc agtttcattg gacttatctc ggtgataaat gggataaaaa gacgaagcaa
      541 tttagtgtag aacagcgttt tgaaagagta ctgccagctg gtgaaatatg gtactcagct
      601 actggaattt cacaaaagaa gatggatgta gattatgatg gacttgtatt aactctggac
      661 actcgatctg tagggaaaaa tgaatcaatt agaattaata cgattttccc cagtacctat
      721 ctttatagcc cagttattaa tgatcaagga tttacaaaaa taaatgaatt agataaaata
      781 aataaacggg tacagaaacg atctacaaca ggatatttgg cattattatt ctcgattgga
      841 tcaatgattt tgtttggctt tcgattttca caatatggaa aagcgcatcc agtaaagaaa
      901 tcgagtgaaa atgggaataa tttcaacttt ccttccaatc accatcccgc tctggtctca
      961 tttttttatt cgggtcataa ggtcactggg catacaatat tagcctcgct tatcggactg
     1021 tcgagcaagg gatactatac aataaaggat attgaaaaaa cgaagaaatc tttttttcga
     1081 aagaaagaaa agacaaagaa atatattgaa gtaattcgaa ttgaaaaacc cgtagaaaat
     1141 aaactccatc aattggacca gttactattg aatcggataa ataaatgttt acggaaagaa
     1201 aaaaatacac tcgaaaagat ttttaaagaa atcggtgaag ataatggatt tatgaaatcc
     1261 ttgaagaaag ccattgataa gaatatcgat aataaccaat gggttgagaa accttcatca
     1321 aagagcacca aaatttattt ttttctgcat gttattatga ttttggttat gatattttta
     1381 tttactgcta acaccctcgc cgggatatta tcaatcatat ttttattatt gatgggactt
     1441 ttcggtagtt ttggcgttat gcgtttatca gtagaagcaa aatcatatcg tcattactgg
     1501 agagaattca gtgcccaatt gcaaaaagga ggattaccaa aaaatcacga tttagatatg
     1561 aatgaaattt tacagtatgc gattgtgatc ggttgtgata aaaaggtaaa ggggttactg
     1621 aaagatttta cacccattgg tgataatcat gccttttact ggtatcacgg agtgggtgat
     1681 ggaaatctgg atatggattc atttacaagt agttttgcag gaatgattga tactggtaca
     1741 gctgtcagcg ctggttttgg tggtgatggc ggtgctggcg gtggcggagc cggtggcggt
     1801 ggcggcggcg gagcaggtta aggtaattg
//
LOCUS       DTVV01000166            3613 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1421264, whole genome shotgun sequence.
ACCESSION   DTVV01000166 DTVV01000000
VERSION     DTVV01000166.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1421264"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /gene="ssb"
                     /locus_tag="EYO86_01950"
     CDS             complement(<1..252)
                     /gene="ssb"
                     /locus_tag="EYO86_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIC41830.1"
                     /translation="MSGSVNKVILLGNLGRDPEIRSMQSGKKMASFSIATSKRWKDRS
                     TQEQKENTSWHNIVVFNEGLVDVIEKYIKKGSKIYVEGEL"
     gene            314..3121
                     /gene="uvrA"
                     /locus_tag="EYO86_01955"
     CDS             314..3121
                     /gene="uvrA"
                     /locus_tag="EYO86_01955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIC41831.1"
                     /translation="MNLDKIIIKGAREHNLKNIDLEIPKNKMIVITGVSGSGKSTLAF
                     DTIYSEGQRKYVESLSAYARQFLQMMNKPDVDSIDGLSPAISIEQKTTQKNPRSTVGT
                     VTEIYDYLRVLFARVGVPYSPTTGLPIKSQSVSEITDLIIANNKNLRIYILSPIVRDR
                     KGEYKKEIEEIKRKGFQRLKIDNKIYDIDDIPKLKKNYKHNIDILVDRLVIKKEIQQR
                     LSESIETALNLSDGLIYVENIDTKKINIYSSKFACPVSGFSIEDIEPRLFSFNAPQGA
                     CEECDGLGIEKYFDDKKIIPDNSRSLLEGAIKPWETKVFGYQKKMFLETIGRIFREFK
                     IKTKIPWKDIPLKIQNLILYGDENNQIKSLTKFEGIINFIDRKYSETERWWVQYELEK
                     YLSERNCEVCRGFRLNEKALAVKIYNHHIGNVTQKSIKKCLNWFKKLSSKLDANQSKI
                     AESIIKEIHDRLNFLNKVGLDYLSLSRASKTLSGGESQRIRLASQIGSGLTGVLYVLD
                     EPTIGLHQKDNQQLIQTLFRLRDLGNTVIVVEHDEEAIRSADHIVDIGVKAGIHGGEI
                     IAEGKLLQILKNKKSLTSQYLNKIKLIEVPKKRRQIDKNKIIKVYKANTNNLDNITVI
                     FPLSTFTCITGVSGSGKSSLVFDTLYQRLDEFFNRSLNKEEKNYNVMGVKNIDKIIDI
                     NQSPIGRTPRSNPATYTGCFTPIRDWYAGLNESAARGYKPGRFSFNVKGGRCENCEGD
                     GVKKIEMHFLADVYVECDTCKGKRYNRETLEIRYKDKSIADILNMTVEEGYIFFNKIP
                     NIKQKLQTLMDVGLDYIKIGQSATTLSGGEAQRIKLSKELSKRSTGKTLYILDEPTTG
                     LHFEDVNKLLKILHTLVNEGNTVIVIEHNLDVIKTADHIIDLGPLGGEKGGKIVFEGS
                     PEDIIMDKQSYTGKYLKKYLN"
     gene            3135..>3613
                     /locus_tag="EYO86_01960"
     CDS             3135..>3613
                     /locus_tag="EYO86_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41832.1"
                     /translation="MASILQSLNKTIIAGIILTLILFFIIFSIWPESNTWLSDTYFWG
                     TVVFRYLHVLSGIMWIGLLWYFNFVQIPNMPNIPDDQKPAIGKVIAPAALFWFRWAAL
                     ATILTGLEVAYLNGYILQAMTLGLMGGDAKSITIGIGMWLGIIMAYNVWMVIWPNQK"
BASE COUNT         1412 a          466 c          611 g         1124 t
ORIGIN      
        1 taattcaccc tctacataaa ttttagagcc tttttttata tacttttcaa tgacatcaac
       61 taaaccttca ttaaaaacaa caatattgtg ccatgaagta ttttcttttt gttcctgagt
      121 actacgatct ttccaacgtt ttgaagtagc gattgaaaat gatgccattt tttttccaga
      181 ttgcatcgag cgtatttcag gatctcttcc aaggttacct aacaaaatta ctttattaac
      241 actaccactc atttaaaatc cttatatata aatatataac aagaaatgag aaattagcta
      301 ataaaaatta tcaatgaatc tggataaaat tatcattaag ggagctcgtg agcacaactt
      361 aaaaaatatt gatctagaaa tacctaaaaa taaaatgatt gtaataactg gcgttagtgg
      421 gtcaggaaag tcaactttag cttttgatac aatttattca gaaggtcaaa gaaagtatgt
      481 tgaaagttta tcagcatatg caagacaatt tcttcaaatg atgaacaaac cagatgtaga
      541 tagtatagat ggactctctc ctgctatatc tattgaacaa aaaacaactc aaaaaaaccc
      601 aagaagcaca gtaggaacag taacggaaat atatgactat ttgagagttc tttttgctcg
      661 tgttggtgtt ccttattcac cgacaacggg tttgcccatt aaatcccaaa gtgtaagtga
      721 aattaccgac ttgataattg ctaataataa aaatcttcgt atatacattt tatcccccat
      781 agttagagat agaaaaggtg aatacaaaaa agaaattgaa gaaataaaaa gaaagggatt
      841 tcaaagacta aagattgata ataaaatata tgacattgac gatattccta aattaaaaaa
      901 aaactataaa cataatattg atattcttgt tgatagactt gtaattaaaa aagaaattca
      961 acaacgatta tctgaaagta tagaaactgc attaaatttg tctgatggat taatttatgt
     1021 tgaaaatata gatacaaaaa aaataaatat ttattcttca aaatttgcct gtcctgtatc
     1081 aggtttttca atagaagata ttgaaccaag attatttagt tttaatgccc ctcaaggagc
     1141 ttgtgaagaa tgtgatggat tgggtataga aaaatacttt gacgacaaaa aaattatccc
     1201 tgataactca agatctttat tggaaggagc tataaaacct tgggagacaa aggtttttgg
     1261 ctatcaaaaa aaaatgtttc ttgaaacaat aggaaggatt ttcagggaat ttaaaattaa
     1321 aactaaaatt ccttggaaag atataccttt aaaaatccaa aacttgatcc tatatggtga
     1381 cgaaaataat cagattaaat ctttaacaaa atttgaagga atcataaact ttattgatag
     1441 aaaatatagt gaaacagaaa gatggtgggt gcaatatgaa ttggaaaaat acttatccga
     1501 aagaaattgt gaagtatgta ggggatttag gctcaatgaa aaagcactag ctgtaaaaat
     1561 ttacaatcat catataggga atgttacaca gaaatctata aagaaatgtt taaattggtt
     1621 taaaaaatta tcttctaaac ttgatgccaa tcaatcaaaa attgcagaaa gtataattaa
     1681 agaaattcat gatagattga attttttaaa taaggttggt cttgattact tatctttatc
     1741 aagagccagt aaaacacttt caggaggtga aagccaaaga ataagacttg caagtcaaat
     1801 aggttcagga ttaacaggag ttttatatgt tttagatgaa cctactattg gattacatca
     1861 aaaagataat caacaactca ttcaaacttt atttagatta agagatttag gtaatacggt
     1921 aattgttgta gaacatgatg aagaagctat cagaagtgct gatcacatcg ttgacatagg
     1981 agtaaaggcc ggtattcatg ggggtgaaat aattgctgag ggaaaacttt tacaaatatt
     2041 aaagaataaa aaaagtttaa catctcaata tttaaataaa attaaactta ttgaagtccc
     2101 caaaaaaaga agacaaattg ataaaaacaa aataataaaa gtttataaag ctaatactaa
     2161 taatttagat aatataactg taattttccc tctaagcact ttcacatgca ttaccggagt
     2221 atcaggaagt ggaaaatcat ctttagtttt tgatacattg tatcaacgtt tggatgaatt
     2281 ttttaataga tcattaaata aagaggaaaa aaattataat gtcatgggtg taaaaaatat
     2341 tgataaaata attgatataa accagtcccc tataggtaga actccaagat ctaatcccgc
     2401 aacatataca ggttgtttta ctcctattcg tgattggtat gcaggcttaa atgaatcagc
     2461 tgccagagga tataagccag gtagattttc atttaatgtc aaaggtggac gatgtgaaaa
     2521 ttgtgaagga gatggagtta aaaaaattga aatgcatttt ttggcggatg tatatgtaga
     2581 gtgtgatacc tgtaaaggaa agaggtataa tagagaaaca ttagaaataa gatacaaaga
     2641 taagtctatt gcagacattt tgaatatgac cgttgaagaa ggttatatct tctttaacaa
     2701 aataccaaat attaaacaaa aattacaaac acttatggat gttggtttag attacataaa
     2761 gataggtcaa tcagccacca cactttcagg aggtgaggct caaaggatta agctttctaa
     2821 agaactatca aaaagatcaa ctggtaaaac cttgtatata ttggatgaac caacaacagg
     2881 tctacatttt gaagatgtga ataagttatt aaaaatcttg catactcttg taaatgaagg
     2941 aaatactgta atagtcattg aacataattt ggatgtaatc aaaacagcag accatattat
     3001 tgatttagga cctttaggag gtgaaaaagg ggggaaaatt gtatttgaag gttctccaga
     3061 agatattata atggataaac aaagctatac aggaaagtat ttaaaaaaat atttaaatta
     3121 aattggaggt ttaagtggca agtatattac aatcactaaa taaaactatc attgcaggaa
     3181 ttattcttac tttaattctt ttttttataa ttttttcaat atggccagaa agcaatactt
     3241 ggctttcaga tacatatttt tggggaacag tagtttttcg ttatttgcat gttttatctg
     3301 gcataatgtg gataggactt ctttggtact ttaactttgt acaaattcca aatatgccaa
     3361 atataccaga tgatcaaaaa ccagctattg gtaaagttat agctcctgct gcattatttt
     3421 ggttcagatg ggcagcatta gcaacaatat taactggttt agaagttgca tatttaaatg
     3481 gatatatact acaagcaatg actcttggcc ttatgggcgg tgacgctaaa agtataacaa
     3541 ttggcattgg aatgtggtta ggtatcataa tggcctacaa tgtatggatg gtaatttggc
     3601 caaatcagaa aaa
//
LOCUS       DTVV01000167            1570 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1421381, whole genome shotgun sequence.
ACCESSION   DTVV01000167 DTVV01000000
VERSION     DTVV01000167.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1421381"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="EYO86_01965"
     CDS             complement(<1..829)
                     /locus_tag="EYO86_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HIC41833.1"
                     /translation="MVSIATLGSHCALQVLKGAKDEGFKTILLCEKKREHLYRRFGFI
                     DEIFLMDSFLELFEQNCESMLQENNSILIPHGTLISQMSSEQIESIKTPVFGNKYILR
                     WESDRALKEQLMKEAKLNVPKSIPNSEQINSLVIAKRHGAAGGKGYFLASSEEEYNKK
                     RDKLVKQGLISGDNDLYIQEYSSGVLAYLQFFYSPLKNELEFFGVDQRHESDIDGLAR
                     IPAENQLATDKTSSFNVIANSPLVLRESLLDDVYKMGENFVEASAKLVPPGMNGPFCI
                     "
     gene            995..>1570
                     /locus_tag="EYO86_01970"
     CDS             995..>1570
                     /locus_tag="EYO86_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HIC41834.1"
                     /translation="MIETELGDWRRTHYSSELGHNLDKSDVIVMGWISSVRGHGNISF
                     VTIIDKMGEIQIVAKKDSCPDDVFENISKLKPHSCIGLKGEVKASEKSPTGVEIIPKE
                     LKIFSTVEKIPPFDPNAKTVKNIDTRLEIRAVDLRRSSLQKIFQARNEVLKSIRSYFS
                     EQSFTEINTPKMIATATEGGAALFPIFYYNKE"
BASE COUNT          543 a          266 c          256 g          505 t
ORIGIN      
        1 ctatacaaaa tggaccgttc attcctggtg gaacaagttt tgctgatgcc tcaacaaaat
       61 tttcacccat cttataaaca tcgtcaagta atgattcacg tagtacaagt gggctatttg
      121 caataacatt gaatgaagat gttttatcgg ttgcaagttg gttttccgct ggaattcttg
      181 ctagtccatc aatatcagat tcatgtcttt gatcaacacc aaaaaattca agttcgttct
      241 taagtggcga ataaaaaaat tgtaggtaag ctaaaacacc agatgaatac tcttgaatat
      301 acaaatcgtt atcaccagaa attaatcctt gcttaactaa tttatcacgt tttttattat
      361 actcctcttc gctggaagca agaaagtaac cttttccacc ggctgcacca tgtctttttg
      421 caatgacaag actattgatt tgttcggaat taggaattga ttttggaacg ttaagttttg
      481 cctccttcat tagctgttct tttaatgctc tgtcagattc ccaacgtaaa atatacttgt
      541 taccaaacac aggtgtttta atagattcaa tttgctctga actcatttgt gatattagtg
      601 taccatgagg aattagtata gaattatttt cttgtaacat agattcacaa ttttgttcaa
      661 acagttctag aaatgaatcc ataagaaata tttcatcgat aaacccaaag cgtctataaa
      721 gatgctccct ttttttctca cataacaaaa ttgttttaaa tccctcatcc ttggcacctt
      781 ttaaaacttg taatgcacaa tgcgagccca aggttgctat tgataccaaa ttatattatt
      841 actaaaaaaa tacacaatat gaaactatgt atctaagctg ggcaaaaccg gctacaaata
      901 cactagagtt tttgtaacac caaacctgaa ttttaccata aagtgacaaa ttctaaaaaa
      961 atcataataa atagtcaatc agaaaaacca cattatgata gaaactgaat taggagattg
     1021 gaggagaact cattattcga gtgaacttgg tcataattta gataaatcag atgtaattgt
     1081 tatgggatgg atttcaagtg ttagagggca tggaaatatt tcgtttgtta cgattattga
     1141 taaaatggga gaaattcaaa ttgttgcaaa aaaggactcg tgcccagacg atgtatttga
     1201 aaatatttct aaattaaaac cacattcttg tatagggtta aaaggggagg ttaaggcttc
     1261 tgaaaaatca cctactggtg ttgaaattat acccaaagag ctcaaaatat tttctaccgt
     1321 tgaaaaaatc cctccatttg atccaaatgc aaaaactgta aaaaacattg acacccgatt
     1381 agaaattagg gctgttgatc tgcgtagaag ttccctacaa aaaatttttc aagcacgcaa
     1441 tgaagtgctt aaatcaatac gaagttattt ttcagaacag agttttactg aaattaatac
     1501 accaaagatg attgctactg ctactgaagg tggtgctgcg ttgtttccaa ttttttatta
     1561 caataaagag
//
LOCUS       DTVV01000168            1625 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1424452, whole genome shotgun sequence.
ACCESSION   DTVV01000168 DTVV01000000
VERSION     DTVV01000168.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1424452"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="EYO86_01975"
     CDS             complement(<1..533)
                     /locus_tag="EYO86_01975"
                     /inference="COORDINATES: protein motif:HMM:PF13181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC41835.1"
                     /translation="MNINSKIKEALLKKSQGDISGAESIFLEILDFSPLQYEVLINLG
                     LIYVHKKMYEASIYYFKQASEADKNNIDPYINLGNVYTLKKNYTNAIQFFELAHKIDQ
                     TNLNVINNLSFLYNEIDKLIESKKIITIGLKIDPTNYFLLNLLGLIFVKKEKISEAID
                     YYIQSIKSNPNYWDAYS"
     gene            618..1226
                     /locus_tag="EYO86_01980"
     CDS             618..1226
                     /locus_tag="EYO86_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41836.1"
                     /translation="MTEQKVDLNLFFPTPVWTSIINNYKHVNQKIFSYIDELKTNDPE
                     GVRRSNFKGWHSKDFELNNENVIIFVNAISTKLNKALIDMNWDLKNQTVKITSMWSII
                     NQKHALNERHIHGNNFISGAYYVKAPSDCGNICFYDPRSGPTFSHPKTTGPNKLNANA
                     HTIKPEDGLLVLFPSYLHHSVETNLSDEERVVISFNVNLISN"
     gene            complement(1223..>1625)
                     /locus_tag="EYO86_01985"
     CDS             complement(1223..>1625)
                     /locus_tag="EYO86_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036675.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HIC41837.1"
                     /translation="YASFDYELAGYKVNDLVKVSVMINGDPVDALSLIAHRDRSQSRG
                     RALCERLKDLIPRQQFKVAIQAAIGGKIIARETVASFRKDVTQKLYGGDVTRKNKLLD
                     KQKKGKKRMRQFGKVDIPQNAFLNALKMSDN"
BASE COUNT          560 a          258 c          218 g          589 t
ORIGIN      
        1 ttactgtatg catcccaata atttgggttg gatttaattg attgaatata ataatcaata
       61 gcctctgaaa ttttttcttt ttttacaaaa atcaatccaa gcaaatttaa aagaaaataa
      121 tttgtgggat ctatttttaa accaatagta attatttttt ttgattctat taatttatca
      181 atttcattgt acaaaaaaga tagattattt attacattca aatttgtttg gtctatcttg
      241 tgcgctagtt caaaaaattg gattgcattt gtataatttt ttttcaatgt ataaacattg
      301 cctaaattaa tataaggatc aatattgttc ttatctgctt cactggcttg tttaaaatag
      361 taaatggatg cttcatacat ttttttatga acgtaaatta ggcctaaatt tattaaaact
      421 tcatattgta gtggtgaaaa atctagaatt tccagaaaaa tactttcagc accagaaata
      481 tccccctgtg atttttttaa tagagcttct ttaattttac tattgatatt cataaaataa
      541 tttcaataac ttatttagat atgtactaaa ttaagatgtt ataatacttt tcgcaataat
      601 atatatatag tgattatatg acagaacaaa aagtggatct aaatttattt tttcccactc
      661 cagtatggac ttctataatt aacaattaca aacatgttaa ccaaaagatt ttttcttata
      721 ttgatgaatt aaaaacaaat gatccagaag gagtaagaag aagtaatttt aaaggctggc
      781 attcaaaaga ttttgaactt aataatgaga acgttataat atttgttaat gctatatcca
      841 caaaactaaa caaagctttg atagatatga attgggattt aaaaaatcaa acagtaaaga
      901 ttacaagtat gtggtccata attaatcaaa aacatgcttt gaatgaaaga catatccatg
      961 gcaataattt tattagcggt gcttattatg ttaaagcgcc ctctgattgt ggaaatattt
     1021 gcttttatga tccacgctca ggtccgacat ttagccaccc aaaaacaact ggtccaaata
     1081 aactcaatgc gaatgctcac accataaaac cagaagatgg gctgctggtt ttgtttccta
     1141 gctaccttca tcactcagta gaaacgaatt tatctgatga agaaagagta gttattagtt
     1201 ttaatgtaaa tctaattagt aactagttat ctgacatttt cagagcattt aaaaaagcat
     1261 tctgaggtat atcaactttg ccaaactgcc tcattctttt ctttcctttt ttttgtttat
     1321 ctaacaattt attttttcta gtcacatccc caccatatag tttttgtgtt acatcttttc
     1381 taaatgaagc aacagtttct cttgctataa ttttaccgcc aatcgccgct tgaatagcca
     1441 ctttaaattg ttgacgaggt attaaatctt tcaatctttc acaaagagcc cggccccggc
     1501 tttgtgacct atctctatgg gcgattaacg acaacgcatc aactggatca ccattaatca
     1561 tcacactaac tttaaccaga tcattaactt tatatccagc gagttcgtag tcaaaacttg
     1621 cataa
//
LOCUS       DTVV01000169            1368 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1428077, whole genome shotgun sequence.
ACCESSION   DTVV01000169 DTVV01000000
VERSION     DTVV01000169.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1428077"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="tldD"
                     /locus_tag="EYO86_01990"
     CDS             complement(<1..873)
                     /gene="tldD"
                     /locus_tag="EYO86_01990"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="responsible for the proteolytic maturation of the
                     E. coli pMccB17 plasmid-encoded microcin B17, an exported
                     protein that targets the essential topoisomerase II DNA
                     gyrase; degrades the E. coli plasmid F-encoded CcdA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIC41838.1"
                     /translation="MRSIKDETSAYSHSSDITEKSIETAGRTVKSILSTSSKSKIVDP
                     IRSNKKHYSEINPLDEFTSEQKTKLLKSIDAYARKLDKRVKQVSASLNGEWQAIHILR
                     KGGYHSGDIRPLVRLNISVTVDNGKRMETGSSGIGGRKSYIEFFNSQIWQMQTKEALR
                     QAIINLKSCPTPAGKMPIVLGPGWPGIILHEAIGHGLEGDFNRKKISVFSELLGKKIA
                     SKNVTVVDDGTINDRRGSITIDDEGTPSQCTTLIENGIMVGHMQDRLNASLMKTKSTG
                     NGRRQSFEFLPMPRM"
     gene            950..1368
                     /locus_tag="EYO86_01995"
                     /pseudo
     CDS             950..1368
                     /locus_tag="EYO86_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
BASE COUNT          443 a          231 c          193 g          501 t
ORIGIN      
        1 cattctaggc attggaagaa attcaaaact ttgtcttcta ccatttccag ttgattttgt
       61 tttcattaaa cttgcattta atctatcctg catatgtcct accattattc cattttcaat
      121 tagtgtggta cattgacttg gcgttccttc atcgtcaatt gtaattgaac ctcttctatc
      181 attaatagtt ccatcatcaa cgacagtaac attttttgac gcaatttttt ttcctaaaag
      241 ttccgaaaac actgaaattt tctttctatt gaaatcacct tctaatccat gaccgatagc
      301 ttcatgaaga ataataccag gccaacctgg ccctaaaact attggcattt ttcctgctgg
      361 cgttggacat gactttaaat taattatcgc ttgtctcaaa gcctctttag tttgcatttg
      421 ccagatttga gaattgaaaa attctatata actttttctt cctcctatac cactggatcc
      481 ggtttccatt ctttttccat tatcaactgt gactgaaata tttaatctta caagtggtct
      541 gatatctcct gaatggtacc cgccttttct taaaatatgt atagcttgcc attctccgtt
      601 aagagaagct gatacttgtt taactctttt atctagtttt ctcgcatatg catctattga
      661 ttttaataac ttagtctttt gttccgaagt aaattcatca agtggattaa tttctgaata
      721 atgtttttta tttgatctaa tcggatcaac aatttttgat ttagaactag ttgataaaat
      781 tgatttaact gttcttcctg cagtctcaat acttttttca gtaatatctg aagaatgaga
      841 atatgctgat gtctcatctt ttattgatcg caaaccaaaa cctttattaa tggatgaact
      901 ggcatttttt atttttttat catcgaatat aaagttttca gattgtcgaa attctaaaaa
      961 taattcaatt cctagcgctg ttttattacg tgacacatta aaaaaagtaa aaacactaag
     1021 agaggctaaa tcttcaatat atggcggtaa ggtaaaagtt gatggtgtta tacaaaaatc
     1081 tcttcatcac tctattggtt taatggatac tatagaatta gaaggaacca cagatatgta
     1141 tagattggta cccaaagatg gacgtatact tgtaccaata aaaattaatg ctactgaaaa
     1201 atctaaaaaa cttgttaaag taaaaagtaa aacaactatt aaaggtggaa aaacacaaat
     1261 tggatttcat gatggaagaa ctataattac tgatacaaat gtaaatgttg atgatacatg
     1321 cttattacaa attcctgaac aaaaaatttt agaaacgatc aagtttga
//
LOCUS       DTVV01000170            1075 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1428789, whole genome shotgun sequence.
ACCESSION   DTVV01000170 DTVV01000000
VERSION     DTVV01000170.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1428789"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="EYO86_02000"
     CDS             <1..776
                     /locus_tag="EYO86_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41839.1"
                     /translation="REQNKYSVNNFTINPDNKSYKSLCNYIHPNFFLLENNSHEENIK
                     IDNVRNILKFLNKTTYNSNIKIVMIDNAEYLNINSSNALLKVLEEHNANTFFFIIHNS
                     SNRILDTIKSRCIQFKFFFTLSERKSILKNIIKQYRNNFSIDEINDNFYFDTPGNVLK
                     YLEILSESNVDYTKDKLSCISYLIDKYRQKKDSQLLIFISLMIELFYNELSLKNNKKL
                     HIYFYHKFKILKQINDVKKFNLDKNNLFISLQEMLKNES"
     gene            766..>1075
                     /locus_tag="EYO86_02005"
     CDS             766..>1075
                     /locus_tag="EYO86_02005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038821.1"
                     /note="methionine--tRNA ligase; MetRS; adds methionine to
                     tRNA(Met) with cleavage of ATP to AMP and diphosphate;
                     some MetRS enzymes form dimers depending on a C-terminal
                     domain that is also found in other proteins such as
                     Trbp111 in Aquifex aeolicus and the cold-shock protein
                     CsaA from Bacillus subtilis while others do not; four
                     subfamilies exist based on sequence motifs and zinc
                     content; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIC41840.1"
                     /translation="MSHNNFYITTPIYYPSGKPHMGHAYSSILADVFARFKRNDNYKV
                     FFLTGTDEHGLKIQKAAEKNGLDPLTYCNKISRIFKDLTKKLNLSNDDFIRTTEKRHY
                     K"
BASE COUNT          443 a          122 c          123 g          387 t
ORIGIN      
        1 atagagaaca aaacaaatat tctgttaata attttactat taatccagat aacaaaagtt
       61 acaaaagttt atgtaattat atacatccaa attttttttt gcttgaaaat aattctcatg
      121 aggaaaatat taaaattgat aatgtaagaa atattcttaa atttctaaac aagacaactt
      181 ataattcaaa tattaaaatt gtaatgatag ataatgcaga atatttaaac attaattcat
      241 ctaatgcatt acttaaagtt ctggaagaac ataatgctaa tacatttttc tttattattc
      301 ataatagctc aaatagaatt ttggacacta taaaatctcg ttgtattcaa tttaaattct
      361 ttttcacctt atctgaaaga aaaagtattt taaaaaacat tattaaacag tataggaata
      421 attttagcat agatgaaatt aatgataatt tttattttga tacaccagga aatgttttaa
      481 aatatttaga gattttaagt gaaagcaatg ttgattatac taaagataaa ttatcttgta
      541 tttcttattt aattgataaa tacagacaga aaaaagactc ccaattatta atttttattt
      601 ctttgatgat agaacttttc tataatgaat tgtctttaaa gaataataaa aagttgcaca
      661 tttattttta tcacaaattt aagatactaa aacaaattaa tgacgtgaaa aaatttaatt
      721 tagataaaaa taatctgttt atttcattgc aagaaatgct caaaaatgag tcataataat
      781 ttttatatta caacacctat ttattaccct tcagggaaac cccatatggg gcatgcctat
      841 tcaagcattc tcgctgatgt ttttgctaga tttaagcgaa atgataatta caaagtcttt
      901 tttctaacag gaacagatga gcacggtttg aagattcaaa aagctgctga aaaaaatggt
      961 ttagatcctt taacttattg caataaaatt tctaggatat ttaaagactt aacaaaaaaa
     1021 ttaaatttat ctaacgatga ttttattaga acgacagaaa aaaggcatta caaat
//
LOCUS       DTVV01000171            1592 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1428987, whole genome shotgun sequence.
ACCESSION   DTVV01000171 DTVV01000000
VERSION     DTVV01000171.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1428987"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="EYO86_02010"
     CDS             complement(<1..286)
                     /locus_tag="EYO86_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIC41841.1"
                     /translation="MTAYAIVQINITNPEDYKEYLTQVTSIVEKYGGEYIVRGGKFEV
                     MLGSWDYERTVVVKFPSYDVAMNWYHSEEYAPVRKIREDNSEGNLIIVEGK"
     gene            867..>1592
                     /locus_tag="EYO86_02015"
     CDS             867..>1592
                     /locus_tag="EYO86_02015"
                     /inference="COORDINATES: protein motif:HMM:PF00106.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC41842.1"
                     /translation="MKKIKYVIITGCSTGIGYETAKYLKEKNFKVIVSCRKNKDVKRL
                     SKEFDYSIQLDTSSSSSIKKAFILIQKIVKNDLVYGIFCNAGYGQFGAVEDLTRALIR
                     EQFETNLFGHIELINLFIPFMRKQNEGRIIFNSSVLGLVSFPFSSAYNASKFAMEAFA
                     RSLRLELNKTNIKVSLIEPGPIEANFNINALKNLKRVKINNSVYKNKYYKMMKFLANG
                     RGFFTLPALSVAKKVHIALKSKKP"
BASE COUNT          534 a          231 c          235 g          592 t
ORIGIN      
        1 atttgccttc aactattatc aagtttcctt cagagttatc ctctctaatt ttcctgactg
       61 gtgcgtattc ctctgaatga taccagttca tggcaacatc ataacttgga aacttgacca
      121 caacagttct ttcataatcc catgacccaa gcataacttc aaacttgcct ccccttacaa
      181 tgtattctcc tccatatttt tccacaatag atgttacttg agttaagtat tccttgtagt
      241 cttctgggtt ggtgatgttt atttgtacga ttgcgtaagc tgtcatatct actggcgcat
      301 aagtattaat gctaatcatt tatagcattt gtcgtgtcga ttcttttcac tctgactgaa
      361 aattaattgt tacatatcaa atcagacagg ttcgagatgg aattatggta atattatgga
      421 tgttaatttt agttaactaa tttataatta attttttcct accaccaatc acgaggaatg
      481 ttgagagtaa aatctataat ataaattaga ggtggtttga caactaattg tgaaaatata
      541 ttgtgttatt atattatatt aatgaaaaat cttgaaagat aatatgtgaa aaaacatgat
      601 gtctcataat taagttattt actatgagtt tttttccatt tctttttcat tttagtccat
      661 tctttatatc ttttatctct tgatgcattg gattttttta gctcattctt aattccctca
      721 agctcttttt taatttgatt caaaatcttg cctacttttt ctgtcatact ttacctcctt
      781 cataactata ccatcattcc ccaaaaagtt aagggggtta tttttaggtt aacacatggt
      841 ttcattaggg gtatagttaa ggaggtatga aaaaaataaa atatgtcatt atcacaggct
      901 gttctactgg tataggctat gaaactgcaa aatatttgaa agaaaaaaat tttaaagtta
      961 tagttagttg tcgcaagaat aaagatgtta aaagattatc aaaagaattt gattattcaa
     1021 ttcagctaga tacatcttcc tccagcagta ttaaaaaagc atttatttta atacaaaaaa
     1081 tagtgaaaaa tgatttagtt tacgggatat tttgtaatgc cggttatgga caatttggtg
     1141 cagttgaaga tttaaccaga gcattaattc gtgaacaatt tgaaacaaat ctatttggac
     1201 atattgaact gataaattta tttatccctt ttatgagaaa acaaaatgag ggaagaatta
     1261 tattcaacag ttctgtgtta ggtttagtat cttttccttt tagttcagct tataatgctt
     1321 ctaaatttgc aatggaagca tttgctagaa gtttgcgatt agaattaaac aaaacaaata
     1381 ttaaagtttc gttaatagaa ccaggcccta ttgaagctaa ttttaatatt aatgctttaa
     1441 aaaatttaaa acgtgtaaaa ataaataata gtgtttacaa aaataaatat tataaaatga
     1501 tgaaattttt agcgaatgga aggggttttt ttacgcttcc tgccctttct gtagctaaaa
     1561 aagttcatat agctctaaaa tcaaaaaaac ca
//
LOCUS       DTVV01000172            1174 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1430094, whole genome shotgun sequence.
ACCESSION   DTVV01000172 DTVV01000000
VERSION     DTVV01000172.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1430094"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /gene="nhaA"
                     /locus_tag="EYO86_02020"
     CDS             <1..827
                     /gene="nhaA"
                     /locus_tag="EYO86_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIC41843.1"
                     /translation="IINFETGNTLRGWAIPSATDIAFSIGVLSLLGKRVPLSLKIFLM
                     ALAIIDDLGAIIIIAFFYSTELQYMYLILMIGSFLALLIINKFGVKKFAPYLLAGLFL
                     WYFTHESGIHSTISGVVLALTIPHRVKEKDFSLLLKLEHILSPYVAFGIMPLFALANA
                     GVSLEGVSTKTLMTPVPLGILCGLFFGKQLGVFLFSYLSIKLKFAEMPSNANWIKIYG
                     VGILTGIGFTMSLFVGNLAFIEDTQHMDGVKIGVLCGSLLSAFAGYFLLLIVSNKK"
     gene            874..>1174
                     /locus_tag="EYO86_02025"
     CDS             874..>1174
                     /locus_tag="EYO86_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIC41844.1"
                     /translation="MISFLGNAVSANYEKLAYDFKFKDLDGNTLSLSEYKGKVIVVVN
                     VASQCGFTNQYEDMQKVWEKYQEKGVVMLGVPSNDFGKQEPGSNEDIKNFCEAKFG"
BASE COUNT          364 a          165 c          218 g          427 t
ORIGIN      
        1 taattatcaa ctttgaaact ggtaatacgc ttagaggatg ggcaatacca tccgcaactg
       61 atattgcttt ttcaattggt gtattgtcat tattaggtaa gcgagttcca ctttctttaa
      121 aaattttttt aatggccttg gcaataatcg acgacttggg tgcaattata atcatagcct
      181 tcttttattc aactgagctt caatacatgt atttaatttt aatgatagga tcttttctag
      241 ctttacttat tatcaataaa tttggagtga aaaaatttgc gccttatttg ttggcaggtc
      301 tcttcctttg gtattttacc cacgaatctg gaattcattc gacaatatcg ggagttgttt
      361 tagctctcac aataccccat cgagttaagg aaaaagactt ttcattattg ttaaaattgg
      421 aacatatttt atcgccctat gttgctttcg gaattatgcc tctttttgcg ttagcaaatg
      481 ctggtgtctc gcttgagggg gtatcaacaa agacgttgat gacgcctgtc cctttgggaa
      541 tattatgtgg attatttttt ggaaaacagc tcggtgtatt tttattttcc tacttgtcta
      601 taaaattaaa attcgcagaa atgccttcaa acgcaaactg gattaaaatt tatggagttg
      661 gaattttaac aggcatagga ttcactatga gtctttttgt tggaaattta gcatttattg
      721 aggacacaca acacatggat ggagttaaaa ttggagtttt gtgtggatct ttactatctg
      781 catttgcagg atatttttta ctattgattg ttagtaataa aaaataatac tctcttaatg
      841 ataaaaaaat caaaactatt tttattattt attatgatct cctttttagg aaatgctgta
      901 agtgccaatt atgaaaaact agcttacgat ttcaaattta aagatcttga tggtaacact
      961 ttaagtctat ctgaatataa aggtaaagtt attgttgtag taaatgtggc aagtcaatgt
     1021 ggctttacca atcaatatga agatatgcaa aaagtatggg aaaaatatca agaaaagggc
     1081 gttgtaatgc tgggtgtgcc gtctaatgat tttggtaaac aggaacctgg aagtaatgaa
     1141 gatattaaaa atttttgcga agctaaattc ggta
//
LOCUS       DTVV01000173            1343 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1438829, whole genome shotgun sequence.
ACCESSION   DTVV01000173 DTVV01000000
VERSION     DTVV01000173.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1438829"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="EYO86_02030"
     CDS             complement(<1..154)
                     /locus_tag="EYO86_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017969631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HIC41845.1"
                     /translation="MPSFDVVSRLDLQEIDNAVSNVLREIKTRYDFKGSETTLERKDH
                     DLTVVTD"
     gene            complement(181..1233)
                     /locus_tag="EYO86_02035"
     CDS             complement(181..1233)
                     /locus_tag="EYO86_02035"
                     /inference="COORDINATES: protein motif:HMM:PF02646.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIC41846.1"
                     /translation="MLLDQLLIFTLGFLLGGITVAIIIWIVFFRKKIDLQNPDSELSS
                     TLKDLKTSFENFKTEDLKTKGANSVMMQNIVQMSTDTIRDVNTLEKTLTKGSSVIQGN
                     WGQTTCENILKEIGFKKGREYESQKKYIDNDGKEKIPDFVIHLPGDRHYILDSKVSNS
                     DWSDYVKATDKDLKEQALKRHVASVIKHIRELSEAGYSNLKDLNTGKDLNTGEFILMY
                     MPLENSFQSLAEESEKIRVEAFKNNITIVGPSLLQLALRIVEQMWSVDKQAKNTKEAM
                     SIATRMYNKAYNINKSFEGVKKSFTTAQKNIETASIQIESGDGNFISLINNFKQKLGL
                     ITNKQITNKNNDEIEN"
BASE COUNT          430 a          225 c          165 g          523 t
ORIGIN      
        1 catcggttac aacagttaaa tcgtgatcct ttctttccaa agttgtctct gagcccttaa
       61 aatcatatct ggtttttatt tcgcgaagca cattagatac ggcattatca atttcctgca
      121 aatcaagacg actgactaca tcaaaactag gcatggtata acttgctata taagtttgct
      181 ttaattttca atttcatcat tatttttatt agttatttgt ttgtttgtaa ttaaacctag
      241 tttttgttta aaattattta taagtgatat aaaatttcca tctcctgatt caatttggat
      301 agaagctgtt tctatatttt tttgagcagt tgtaaaagat tttttaacac cttcaaaaga
      361 cttgttaata ttatatgctt tattatacat tctagtagca atactcatag cttctttggt
      421 attttttgcc tgcttatcaa ctgaccacat ttgctcaacg attcttaaag ctaattgtaa
      481 caaagaaggt cctacaattg ttatattatt tttaaaagct tctactctga tcttttctga
      541 ttcctcagcc aaagattgaa aagaattttc aagaggcata tacataagaa taaattcccc
      601 agtatttaaa tctttaccgg tatttaaatc ttttaaattt gaataaccgg cctcacttag
      661 ttctctaata tgtttaatca cacttgcaac gtggcgcttc aatgcttgct cttttaaatc
      721 tttgtcagtg gcttttacat aatcagacca atcagaattt gatactttgg agtctaatat
      781 ataatggcga tctccaggca aatgaataac aaaatctggt attttttctt taccatcgtt
      841 gtctatatat tttttttgac tctcatattc tcttcctttt ttaaatccaa tttcttttaa
      901 tatattctca caagttgtct gcccccaatt accttgaatt actgaagacc ccttagttag
      961 ggttttttca agtgtgttaa catctctgat tgtgtctgta ctcatctgaa caatattctg
     1021 catcattact gaatttgcac ctttggtttt taaatcttca gttttaaaat tttcaaaact
     1081 tgtttttaaa tcttttagag tagaagaaag ttctgaatct ggattttgca aatctatttt
     1141 ttttctaaaa aaaacaatcc atataatgat tgctactgtt attcctccca aaagaaaacc
     1201 taaagtaaaa attaataatt gatctaaaag cataaaatta tttatacatt aaaagatatt
     1261 tattgcccat agtgttgaaa aataaacaac caagtatctt ccaactttac caatagaaac
     1321 taaaattaaa aatggaaaaa agg
//
LOCUS       DTVV01000174            2911 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1444724, whole genome shotgun sequence.
ACCESSION   DTVV01000174 DTVV01000000
VERSION     DTVV01000174.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1444724"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="EYO86_02040"
     CDS             complement(<1..168)
                     /locus_tag="EYO86_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIC41847.1"
                     /translation="MVKPSIILVRPQLPENIGMAARAMDNCGLKKLIIVSPREIWPNK
                     IALQSAANSKII"
     gene            complement(177..1757)
                     /locus_tag="EYO86_02045"
     CDS             complement(177..1757)
                     /locus_tag="EYO86_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIC41848.1"
                     /translation="MENKIYLFDTTLRDGQQTTGVNFSVSDKMIIANALDELGVDYIE
                     GGWPGANPTDDEFFARDISFTNSSFTAFGMTRRPGKSAANDPGLNTLINSKAPSVCIV
                     GKASLFQVTEALSIEKEENLLMISDSISEIVNKKKDAIFDAEHFFDGYKFNKNYSMQC
                     VEAAYKAGARWIVLCDTNGGTLPNEVFDIVSDVVKSIPGTNLGIHAHNDTDNAVANTL
                     AAIQAGVRHAQGTINGLGERCGNTNLISLIPTLELKTDYVTNINKEKLQSLTRVSKLL
                     DELLNVSSKKQSPYVGEYAFSHKGGLHASAVEKNPLTYEHIDPELVGNARNVIISGQA
                     GKSNLLNQLNKMSIELNDNQLISILRIIKEKEAEGFSYDTALASFELLVRKELGQIKD
                     FYTLQKFRVTDERRWNAKGELITESEATINVHVAGEEKMTVGIGNGPVNAIDSALRKA
                     LTNFYPSLNNLKLTDYKVMILSAEKGTGAITRVLIESSDETNHHWTTIGVSPNIIDAS
                     YNAIYDSITFKLFHDLNS"
     gene            complement(1820..>2911)
                     /locus_tag="EYO86_02050"
     CDS             complement(1820..>2911)
                     /locus_tag="EYO86_02050"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:PF00749.19"
                     /note="charges one glutamine molecule and pairs it to its
                     corresponding RNA trinucleotide during protein
                     translation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIC41849.1"
                     /translation="TLKFLPVVRRFNFNFINRTLRYFIKKKSQLSSGKPPIYDRSSLK
                     LTKKDIGDLIKVGKKPHWRFKLEDKKIEWHDLVKGKVSFDSKNLSDPVLIREDGSLLY
                     HLPSVIDDIEESITDIIRGDDHITNTAFHIQIFEALKSDIPKFGHHPFLTDEFGKSFG
                     KRLNSLSIASMRKKGYENMTLLNYLLSIGTSSNLTKEKDLKSLITKFNIENLSSSSPK
                     FLINVLQSLNKEIVQSYSFGDVRQKFIDLNIKDVKEDFWNFIKNNINFFSECLEWLEI
                     IKSTNIYDTENSDFLKQAAEVLPDSPYDTQSWEEWTSLIKDKTGKRGKELFKPLRIAL
                     TGREKGPELKYLLPLLNRDYILKKLGFIV"
BASE COUNT          920 a          503 c          387 g         1101 t
ORIGIN      
        1 aattattttt gaatttgctg cggattgtaa agcaatctta tttggccaaa tttctcttgg
       61 agatactatg attaattttt ttaagccaca attatccata gctcttgctg ccatacctat
      121 attttctggt aattggggcc taactaaaat tattgaaggt ttgactatct tcactattat
      181 gaatttaaat catggaataa cttaaaggta atactatcat aaatagcatt gtaagatgca
      241 tcaattatat ttggggatac tccgatggtt gtccaatgat gattggtttc atcagaggat
      301 tctattaata ctctagttat tgcacctgta cctttttcgg cagatagtat cataacttta
      361 taatcagtaa gttttaaatt atttaaactt ggataaaaat tagttaatgc ttttcttaaa
      421 gcgctatcta ttgcattaac tgggccgtta cctattccta ctgtcatttt ttcttcacca
      481 gccacatgta cattaattgt tgcttcggac tctgttatca attctccttt ggcattccaa
      541 cgtctttcat cagtgactct aaatttttgt aacgtataaa aatctttaat ctgaccaagt
      601 tcttttctta ctaataattc gaaactagct aaagcagtat cataagaaaa tccttcagcc
      661 tccttttctt ttataatcct taaaatacta attaactgat tatcatttaa ttcaatcgac
      721 attttattaa gttgattgag caaattagat ttacctgctt gaccagaaat aataacattt
      781 cttgcattgc ctactaactc gggatctata tgttcgtaag tcaaaggatt tttttctact
      841 gctgaagcat gtaatcctcc cttatgagaa aatgcatact ctccaacata aggactttgt
      901 tttttggatg aaacatttaa taattcatct agcaatttag atactctagt aagagattgg
      961 agtttttctt tatttatatt tgtcacataa tctgttttta actcaagagt tggaattaaa
     1021 gagattaaat ttgtatttcc acatctttca cctaaaccat taattgtacc ttgagcatgt
     1081 cgaacaccag cttgaatggc agctaatgta ttagcaactg cgttatcagt atcattatgt
     1141 gcatgtattc ctaaattagt tcccggaatt gattttacta catcagatac aatgtcaaaa
     1201 acttcatttg ggagtgttcc tccatttgta tcacataaaa caatccatct tgcaccagct
     1261 ttataggctg cttctacgca ttgcatggaa taatttttat taaatttata tccatcaaaa
     1321 aaatgctctg catcaaatat agcatccttt tttttattaa cgatctcaga aatactatca
     1381 ctaatcatta ataaattttc ttccttttct atagataggg cttctgtaac ttgaaataaa
     1441 gaagcttttc caactataca aacagaagga gctttagaat ttataagtgt attcagacca
     1501 ggatcatttg ctgcactttt accaggcctt cttgtcattc caaaagcggt aaaacttgaa
     1561 ttagtaaatg aaatatcacg cgcaaaaaac tcatcatctg tagggttggc tccaggccaa
     1621 ccaccctcaa tataatccac tccaagttca tcaagtgcat tagcaatgat cattttatca
     1681 cttaccgaaa aattaactcc agttgtctgt tgcccatctc ttaaagtggt atcaaataaa
     1741 taaatcttat tttccattat tttaggtctg ctaaatgaaa agatattttt tgtcgagtaa
     1801 tttaaattct tcaacaattc tatacaataa agcctagttt ttttaaaata taatccctat
     1861 ttaacaaagg taaaagatat tttaattctg ggcctttttc cctcccagtt aacgctattc
     1921 ttaagggctt aaataattct tttccccttt tgcctgtttt atctttaatt aatgaagtcc
     1981 attcttccca actttgagtg tcataagggc tatcaggcaa aacttcagct gcctgtttga
     2041 gaaaatctga attttcagta tcataaatat ttgttgattt aataatttca agccattcca
     2101 aacattctga gaaaaaatta atattatttt taataaaatt ccagaaatct tcttttacat
     2161 ctttaatatt tagatcaatg aatttttgcc taacatctcc aaaactatat gactgtacta
     2221 tttctttatt taaagattga agcacattga ttaagaattt tggagaggat gaagatagat
     2281 tttcaatatt aaatttagta atcagtgatt ttaaatcttt ttcttttgtt aaattggaac
     2341 ttgtgcctat actcagcaaa taattaagga gcgtcatatt ttcataacct ttttttctca
     2401 tacttgctat ggagaggctg tttaaccttt tgccaaaact ttttccgaat tcatctgtca
     2461 aaaatgggtg atgaccaaat ttaggaatat ctgattttaa tgcctcaaat atttgaatat
     2521 gaaaagctgt atttgttatg tggtcatccc cacgtattat atctgtaata ctctcttcta
     2581 tatcatcaat aacggaaggt aagtgataca ataaacttcc atcttctcta ataagtacag
     2641 ggtcacttaa gtttttactg tcaaaagata ccttgccctt aaccaagtca tgccattcaa
     2701 ttttcttatc ttctaattta aatctccaat gaggcttttt acctacttta attaaatcac
     2761 cgatatcttt ttttgttaat tttagtgagc ttctatcata aatgggcggt ttacccgatg
     2821 agagttgaga ttttttttta ataaaatatc ttaatgttcg attaataaaa ttaaaattaa
     2881 atcttcttac tactggtaag aattttaaag t
//
LOCUS       DTVV01000175            1096 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1450534, whole genome shotgun sequence.
ACCESSION   DTVV01000175 DTVV01000000
VERSION     DTVV01000175.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1450534"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="EYO86_02055"
     CDS             complement(<1..564)
                     /locus_tag="EYO86_02055"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIC41850.1"
                     /translation="MIDQTKLPKSLEYVTYTDYNDVAMAIKNLVIRGAPAIGVAGAFG
                     LVLAALQSSSETIDDFLSDLEKAKEILLETRPTAINLSWGLEQIYDSAFGYAQQDVTV
                     SEIKERLIYVAKQMAEEDVNINKRMGKNGAELFQNNDTIMTHCNAGALATVGYGTALG
                     VIRATKESGKNIKVIATETRPVQQGSRL"
BASE COUNT          318 a          187 c          158 g          433 t
ORIGIN      
        1 aagtctagaa ccctgttgaa ccggacgtgt ttctgtagct attactttga tgttttttcc
       61 actttctttg gttgctcgta taactcccag ggcagtacca tatcccacgg tagctaaggc
      121 accagcatta caatgagtca ttattgtatc attattttga aataattctg cgccgttttt
      181 ccccatcctt ttgttaatat tgacatcttc ttctgccatt tgctttgcaa cataaattag
      241 ccgttctttt atttcagaaa ctgttacgtc ttgttgtgcg taaccaaagg cggagtcgta
      301 tatttgttct agcccccagg atagattgat tgcagtaggt cttgtctcaa ggagaatttc
      361 ttttgccttt tccaaatcag acaggaaatc atcaatagtt tcagatgagc tttgtaatgc
      421 agcaagtact agtccaaatg ctcctgccac accaatagct ggagccccac gaatgactaa
      481 atttttaatg gccattgcaa catcattgta atcagtataa gttacatatt ccaaactttt
      541 tggcagtttg gtctgatcta tcattattac agagttattt ttccattcca ctgtttttag
      601 tatcaatgat tacattatag acggttgata tattatccat tctacatctc taattctaaa
      661 attttcttac atattcgatt tggtcctttg gaatcaatca aatttggttt tttaattttt
      721 aatctttttt tttgggttaa atttgaaatg attttgtcaa ttacttttga ataattgata
      781 ttatcatatt ttccaagata ttcataccaa ctatattttt ctattcgttt agctgaaaaa
      841 tgattttcaa aactatgtga gataactaat gtaggaatat taagagctac tgcttcatat
      901 actgtgattc caaaagaaat tattccaaat tgacatttta taattatttc atgcaagttt
      961 tttgtgttgt caactaaaat gaatctttta tcattttgta aaatatgatg aattttttta
     1021 ctgtttgaat aaaatttccc caaaacaatg aatgctgaaa aatcaaattt agtttttttt
     1081 aattgtttca caatac
//
LOCUS       DTVV01000176            1071 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1459624, whole genome shotgun sequence.
ACCESSION   DTVV01000176 DTVV01000000
VERSION     DTVV01000176.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1459624"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1071
                     /locus_tag="EYO86_02060"
     CDS             <1..>1071
                     /locus_tag="EYO86_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIC41851.1"
                     /translation="DIALPIRNIFRSQQNLKVILDELVEIKPQFNRIRTRNNTYSYDY
                     LILATGSELSYFGQDQWKKFSLGLKNIENATLLRSKILQAFEKAERINDDKTINKLLK
                     FVVIGGGPTGVEMAGAIAELSKNVLTKEFATIDTMATQIILLEAGPSILPTASLRLSI
                     YAKDSLEKMGVEVKCNTVVQNITEFLVSTNNENIEAGTIIWCAGVKVSLLRNLINIET
                     DKIGRVFVNDDLSIPKYPKIFVVGDASHVKDKNGNPLPGLAPVAKQEGRFVAGVIKKY
                     VLNDKTQNKFYYKNRGYLATIGRSKAIVDFGWLTLKGRIGWIFWSLIHIYFLIGFRNR
                     FMVFVNWVWSFLTFSKSARLII"
BASE COUNT          367 a          147 c          201 g          356 t
ORIGIN      
        1 cgatatagct ctgccaatca gaaacatttt tcgttcacaa caaaatctta aagttattct
       61 tgatgagttg gttgaaatta aacctcaatt taatcgaatt agaactagaa ataacacata
      121 ctcatatgat tatcttattt tagctacggg ttcagaacta agttattttg gtcaagatca
      181 gtggaaaaaa ttctcacttg gtttaaaaaa tattgaaaat gctactttac tacgaagcaa
      241 gattttacaa gcttttgaaa aagccgaaag aataaatgat gataaaacaa taaacaaact
      301 tttgaaattt gttgttatag gtggaggtcc gacaggagtg gaaatggccg gcgctatagc
      361 agaattgtct aaaaatgtat taacaaaaga atttgctacc attgacacaa tggctacaca
      421 aattatttta ttagaagcag gtcctagtat attacccaca gcttcactta gattatctat
      481 ttatgcaaaa gatagtcttg aaaaaatggg ggtagaagtt aaatgtaata ctgttgttca
      541 aaatattaca gaatttttgg tttcaaccaa taatgaaaat attgaggcag gaacaattat
      601 atggtgcgcg ggagttaaag tttcacttct tagaaatttg ataaatattg aaacagataa
      661 gattggtagg gtatttgtca atgatgactt gtcaattccc aaatacccta aaatttttgt
      721 tgttggtgat gcctctcatg ttaaagataa aaatggcaac cccttacctg gactggctcc
      781 tgtagctaaa caagaaggca ggtttgttgc tggagttatt aaaaagtatg tgcttaacga
      841 taaaactcaa aataaatttt actataaaaa caggggttat cttgctacca ttgggaggtc
      901 aaaagcaatt gttgattttg gttggctcac tcttaaaggc aggattgggt ggatattttg
      961 gtcattgatt cacatttatt ttttgatagg ttttcgcaac cgttttatgg tttttgtaaa
     1021 ttgggtttgg tcatttctaa cttttagtaa aagtgctcgc ttgataataa a
//
LOCUS       DTVV01000177            1432 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1461187, whole genome shotgun sequence.
ACCESSION   DTVV01000177 DTVV01000000
VERSION     DTVV01000177.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1461187"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="EYO86_02065"
     CDS             <1..442
                     /locus_tag="EYO86_02065"
                     /inference="COORDINATES: protein motif:HMM:PF05193.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIC41852.1"
                     /translation="EIREKRGLVYTISSFAFPFSDTGLFGIYAGTGEKQIEEFIPVLC
                     DELKNSPKTISENEIKKGKAQLKANLLMARERANSRCRSAANQLLYFNRVIEPEEITT
                     KIDNVTKDTVERIAHNILKTPMTIASIGPIKKLETLDKIKSRLN"
     gene            546..>1432
                     /locus_tag="EYO86_02070"
     CDS             546..>1432
                     /locus_tag="EYO86_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC41853.1"
                     /translation="MNLFKNIFSTFSVLSLVVFLTLPVQAKVEGDTIILGAAVSLTGK
                     YTTNGEHTQNGYNMAVQRINDMGGVTVGGKSYKFDIIYYDDESDSGRAAQLAERLIKQ
                     DGVQYMLGPYSSGLTKAMAPVTEENQIPMVEANGASRSLFTKGYKYLFAVLSAANQYL
                     EVAIDLAVEKNGGNPVKIAMAFEQDAFSQDVRIGVVEAAERTGSTIIIDDKLPKELND
                     MAATLAKVKATKPDVLVVSGHSKGALTAIRQISEMKVDVPMLAMTHCDASKLAKQHGK
                     NSEYALCAAQWHKTLSYSD"
BASE COUNT          499 a          229 c          297 g          407 t
ORIGIN      
        1 agaaatacgg gagaaaagag gtttggtgta taccatttcc tcatttgctt ttccattttc
       61 tgatactggc ctatttggaa tttacgctgg tacaggtgaa aaacaaattg aagaatttat
      121 tcctgttttg tgtgatgaat taaaaaatag tcctaaaacc atttctgaaa atgaaattaa
      181 aaaaggcaag gctcaactga aagcaaatct tttgatggca agggaaagag ccaatagtcg
      241 ctgcagaagt gctgccaatc aactgcttta ttttaatcga gttattgaac cagaagagat
      301 tactacaaaa attgacaacg taaccaagga cactgttgaa agaattgccc ataacattct
      361 gaaaacacca atgactattg ccagtatagg gcccattaaa aagctggaaa ccttagataa
      421 aataaaatca cgccttaatt aaatttacta ttgatgccag gttgatatct gtttagaaat
      481 ttagtgttaa attttcaatt ttaaggatat atttaaatag aaaataataa attttgggag
      541 gaaacatgaa tttatttaaa aatatatttt caaccttttc agttctttca ctagtagtgt
      601 ttttgacact gccagtgcaa gctaaagtgg aaggagatac aattattctc ggggctgctg
      661 tttcgttaac aggaaaatat actacaaatg gagagcatac ccagaatggc tataacatgg
      721 ctgttcaacg catcaatgac atgggtgggg taacagtcgg tggaaaatct tacaagtttg
      781 atatcattta ttatgacgat gagtctgact ctggaagagc tgcacaattg gcagaacgtt
      841 tgatcaaaca agacggtgta caatacatgc ttggtccata tagttctgga ttgacaaaag
      901 cgatggctcc agtgacagaa gaaaatcaaa ttccaatggt ggaagctaat ggcgcttcgc
      961 gttcgttatt cacaaaagga tataaatatt tgtttgcggt actatcagca gcgaaccagt
     1021 atttagaggt agcaattgat ttagctgttg aaaaaaatgg aggcaatcct gtaaaaattg
     1081 caatggcatt tgagcaggat gcattctcac aagatgtacg tattggtgta gtggaagctg
     1141 cagagagaac gggttcaaca attataattg atgacaaatt gcctaaagaa ttaaatgata
     1201 tggctgcaac gttagcaaaa gtaaaggcta caaagcctga tgtattggtc gtatccggtc
     1261 actctaaagg tgcattaaca gcaatccgtc aaatttcaga aatgaaagtg gacgtgccaa
     1321 tgttagccat gacacattgt gatgcttcca agttagcaaa acaacatggt aaaaattctg
     1381 aatatgcatt gtgcgcagca cagtggcaca aaactttatc ctacagtgac ag
//
LOCUS       DTVV01000178            1817 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1462203, whole genome shotgun sequence.
ACCESSION   DTVV01000178 DTVV01000000
VERSION     DTVV01000178.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1462203"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(91..1299)
                     /locus_tag="EYO86_02075"
     CDS             complement(91..1299)
                     /locus_tag="EYO86_02075"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC41854.1"
                     /translation="MTKIVKEFFIVNNYRIYLIGISVTFCYSSCIYLFSALLQTWETE
                     LNLSRNNLTFAYSLGLMIHSLLLPYLGKLVDRNHSFIMMWLSPLLLGFSVIYLAFVNT
                     YYEFVIAWLLVSVTSAGCLYTMIFSVLTINLTNNAKNSIAIITLIAGLASTYTFPTAT
                     YLTESFNWRTTVFIFGIVNVLISAPANYFGFKKIIHKQKNFNSNIKAEKQENIFFNPR
                     FWLLCFPLFVIGFNVGAITTHIIPMMQEKNMSLTMAVLIASLFGPGQIIGRILIMTYG
                     HNKSNISLFVVCFYSILIGITFLYFVNISFYLAFLFILFNSAAFGSMAILKPLVQNDI
                     FGKMNFGNIHGILAICFMLGSIAGPWIGSLIWSVGGYNLLIMLFFILAVLGNISALML
                     KKYKTKAGLK"
     gene            1426..>1817
                     /locus_tag="EYO86_02080"
     CDS             1426..>1817
                     /locus_tag="EYO86_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HIC41855.1"
                     /translation="MFTTLARLKDNRMFILAMIVPSMIMLIFSLFNMTAPLDPERAAS
                     AFRLGVVNQDEGLTSPPIRISSRMLGGLGENLPFKINEFKTIDLARAALEVGEVAAVV
                     VFPPEFSKNAIGDENFEIEIWNAQHLSV"
BASE COUNT          666 a          292 c          285 g          574 t
ORIGIN      
        1 caaaaacctc ttcgataatt ctcaacattc ctcgtggttg gtggtaagtg ataaaattaa
       61 attttagaga attatctcaa ataaatttta ttacttaagt cctgctttag ttttatattt
      121 tttaagcatc agagcactaa tattgcctaa tactgctaaa ataaaaaaaa gcataataag
      181 aagattgtat ccgccaacac tccaaattaa tgaaccaatc catggacctg cgatacttcc
      241 taacataaaa caaattgcaa gaataccgtg aatatttcca aaattcattt taccaaaaat
      301 atcattttgg actaatggtt taagtattgc catacttcca aaagctgcac tgttaaataa
      361 aataaataaa aatgctaagt aaaaactaat gttaacaaaa tataaaaaag ttattcctat
      421 taaaatagaa tagaaacaaa ccacaaataa actgatgtta gatttattat ggccataagt
      481 cataattaat attcttccaa taatttgacc gggaccaaat aaggatgcaa ttaaaacagc
      541 catagttaat gacatgtttt tttcctgcat catgggaata atatgagtgg ttattgcacc
      601 aacattaaaa ccaattacaa aaagaggaaa acacaacaac caaaatcttg gattaaaaaa
      661 tatattttct tgtttctcag ctttaatatt tgaattaaaa tttttttgtt tatggattat
      721 ttttttaaaa ccaaaataat ttgcaggtgc actaatcaaa acattaacta ttccaaaaat
      781 aaaaactgtg gttctccaat taaaagattc tgtaagatat gttgctgtag gaaatgtgta
      841 tgtgctcgca agtccagcaa ttaaagtaat aatagcaata gaatttttgg cattatttgt
      901 aagatttatt gtaagtacac taaaaatcat agtatataaa cagccagctg aagttacact
      961 gactaatagc catgcaatta caaattcata atatgtattg acgaaagcta aataaataac
     1021 actaaatccc aatagtaaag gacttagcca catcattata aaactatgat ttctgtcaac
     1081 aagtttacct aaatagggaa gtaataaact atgaatcatt aaacctaatg agtatgcaaa
     1141 tgttaagttg tttcttgata aatttaattc tgtttcccat gtttgaagaa gagctgagaa
     1201 taaatatatg caactgctgt aacaaaaagt tacagatatg ccgattagat aaattctata
     1261 attattcaca ataaaaaatt ctttaacaat ttttgtcaac tatatacatt taatttaaca
     1321 ctcaacattc ctcgtggttg gtggtaggat aattgggata aagagaattt gacaatgatt
     1381 tttaaattag ggtaaatata ctctattgtt attggagata tcaaaatgtt tacaacgctt
     1441 gcacgcctga aagataaccg catgttcatc ctggcaatga ttgtaccatc catgatcatg
     1501 ctgatttttt cgctttttaa catgactgcg cccctcgatc ctgaaagggc cgcttctgcg
     1561 ttcaggctgg gagttgtcaa tcaggacgag ggattgacct ctccgcctat ccggatctcg
     1621 tcacgtatgt tgggcgggtt gggtgaaaac ctacctttta agatcaatga atttaaaact
     1681 atcgatcttg caagagcagc acttgaagtt ggggaagtcg cggctgttgt ggtgttcccg
     1741 cctgagtttt ccaaaaatgc aattggcgat gaaaactttg agatcgaaat atggaatgcc
     1801 caacatctga gcgttag
//
LOCUS       DTVV01000179            1047 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1465259, whole genome shotgun sequence.
ACCESSION   DTVV01000179 DTVV01000000
VERSION     DTVV01000179.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1465259"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="EYO86_02085"
     CDS             complement(<1..950)
                     /locus_tag="EYO86_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC41856.1"
                     /translation="MSEDVQLKSSVGVIGAGIQGICISLCLIKKGFRVTLIDRDDPGK
                     NSASYGNAGHFSPYASVPINRPDILLDIPSMLLSSTGPLALQWNYVLKMIPWFLKFIK
                     NCSKKKMMHTAKYMHQILDLALPAYDELFEDIDISGLVEGKGIIYFWTKKYLKSRELE
                     IRIRNELGVKQQLLTPHEIHDLEPHIKKIYHGGVLYPNARHARNPGKILLKLFDLFLK
                     RGGHYEKQNVQTLRFTSDNKPVIKTDSKFYNFDKVVIACGAFSKKLTDQVDENIPLDT
                     ERGYHVHFKGYDHLLSRPVIFLNRGFGITPMEQGLRVVGTV"
BASE COUNT          331 a          193 c          147 g          376 t
ORIGIN      
        1 acggtaccta ccactcttaa tccttgttcc ataggagtta ttccaaaacc tctatttaaa
       61 aagataactg gtcttgatag taaatgatcg tatcctttaa aatgaacatg atatcctctt
      121 tcagtgtcta atggaatatt ttcatcaact tgatcagtca atttttttga aaatgcgcca
      181 cacgcaataa cgactttatc aaaattgtaa aattttgaat ccgtttttat cactggttta
      241 ttgtctgatg taaagcgtaa cgtttgaaca ttttgttttt cataatgtcc acctcttttt
      301 aaaaataaat caaataactt taataagatc tttccgggat ttcttgcatg tctagcatta
      361 ggatacaaaa cgccgccatg ataaattttt tttatatgtg gttctagatc gtgaatttca
      421 tgcggagtta gcaattgctg cttcacaccc aattcatttc taatcctgat ttctaattct
      481 ctacttttta aatatttttt agtccaaaaa tatattattc ctttaccttc caccaatcct
      541 gaaatatcga tgtcttcaaa aagttcatcg taagctggca atgctaaatc taaaatttga
      601 tgcatatact ttgctgtatg catcattttt tttttactgc aattttttat aaattttaaa
      661 aaccatggaa tcatcttcag cacatagttc cactgtaatg ctaatggtcc agtagaactt
      721 aacaacattg agggtatatc taataatata tctggtctgt taattggtac tgaagcatag
      781 ggcgaaaaat gaccagcatt accataggaa gcagaatttt tcccaggatc atcacgatcg
      841 ataagagtta ctctaaaacc ttttttaata agacaaagtg aaatacaaat accttgtatc
      901 ccagctccaa ttactccaac tgaagatttt aactgaacat cttcgctcat agtatcgcta
      961 gttatatgtt atttaagatt taaatatcgg gggacacgtt atacagcttc tttatgctgc
     1021 cctacatgaa cgactctagg tttagat
//
LOCUS       DTVV01000180            1290 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1469327, whole genome shotgun sequence.
ACCESSION   DTVV01000180 DTVV01000000
VERSION     DTVV01000180.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1469327"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="EYO86_02090"
     CDS             <1..392
                     /locus_tag="EYO86_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIC41857.1"
                     /translation="GGGYDNHKLFWNNMKPNGGGEPGGALADAINSSFGSFADFKEQF
                     SSKTAPIQGSGWGWLVYNPSSSKVEYKAMPNQTSPRTVGLIPLLGLDVWEHAYYLKYQ
                     NKRPDYIAAWWNVVNWEEVDNRLSKTK"
     gene            462..881
                     /gene="pfdA"
                     /locus_tag="EYO86_02095"
     CDS             462..881
                     /gene="pfdA"
                     /locus_tag="EYO86_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HIC41858.1"
                     /translation="MSEEKTQQLLYQMQMLEGYFSELIQKEEAIISLFREASSAVVSL
                     KAIDNKTDSETLVPMGLGTFVKTKLIPNQKLILNVGAGVAIEKDQNYAINFLESRLKE
                     MQVALQEIGGQKQQVSASLEQGKQQMNQLLAASRSKK"
     gene            893..>1290
                     /locus_tag="EYO86_02100"
     CDS             893..>1290
                     /locus_tag="EYO86_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIC41859.1"
                     /translation="MFEKLGNAFSNIAKSLGEKELKEQDIDENLSQLEISLLESDVAM
                     EVIDKIKLNLKEKLVGNKVSRKEIEEFVKKSLIQNISNMFDEAGSFDLLEKIKSKTDP
                     QDPFLILFVGINGTGKTTTVAKIANLLQKN"
BASE COUNT          476 a          188 c          246 g          380 t
ORIGIN      
        1 atggcggtgg atacgataat cataaactgt tttggaataa tatgaaacca aatggcggtg
       61 gagaacctgg cggagcacta gcagatgcga ttaactcttc ctttggatct tttgctgact
      121 ttaaggaaca attttctagc aaaacagctc caattcaagg aagtggctgg ggctggctcg
      181 tatacaatcc atcatctagt aaagttgagt acaaagcaat gccaaatcaa actagtccta
      241 gaactgttgg actgattccg ttgttaggac ttgatgtatg ggagcatgct tactatctaa
      301 agtaccaaaa caaacgacca gactatattg cggcttggtg gaatgttgtt aactgggaag
      361 aagttgataa tagactttct aagacaaagt aaatcctatt ttttattttt ctaaccttga
      421 tgtattaaac gcatttatag ctagtcacta cattttttat aatgagtgaa gagaaaactc
      481 agcaattatt gtatcaaatg caaatgcttg agggttattt ttctgaatta atacagaaag
      541 aagaagcaat catatctctt ttcagggaag catcttctgc agttgtctca cttaaagcaa
      601 ttgataacaa gactgattct gaaacactgg taccgatggg gttaggaact tttgtaaaaa
      661 caaaattaat tcccaatcaa aaacttatct taaatgttgg tgcgggtgtt gccattgaaa
      721 aagatcaaaa ctatgctatc aattttcttg aatcacgcct aaaagaaatg caagtcgctt
      781 tacaggagat aggtgggcaa aaacaacagg tttcagcaag tttagaacaa ggaaaacaac
      841 aaatgaatca attattggct gcttctcgta gcaaaaaata aagaaaaaag tcatgtttga
      901 aaaactaggt aacgcgtttt caaatatagc taaaagcctc ggtgaaaagg agctaaagga
      961 gcaagatatt gatgagaatt tatctcaact ggaaatctct cttttggaat ctgatgtcgc
     1021 aatggaagtt attgataaaa taaaattaaa tcttaaagaa aaattagttg gcaataaagt
     1081 aagtaggaaa gaaattgaag aatttgttaa aaaaagtcta attcaaaata tttctaatat
     1141 gtttgatgaa gctggttcat ttgatttatt ggaaaagata aaatcaaaaa cggatccaca
     1201 agatcctttt cttattttgt ttgttggtat aaatggaaca ggtaaaacta caacagtagc
     1261 aaaaatagct aatcttttac aaaaaaataa
//
LOCUS       DTVV01000181            1842 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1470017, whole genome shotgun sequence.
ACCESSION   DTVV01000181 DTVV01000000
VERSION     DTVV01000181.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1470017"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="EYO86_02105"
     CDS             complement(<1..248)
                     /locus_tag="EYO86_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC41860.1"
                     /translation="MLFSILKKIKFKGKIDFIDYKGNKHSFGQAGPYSKVRFTNKSIE
                     RKLVRNPGLYLGEGYMNKEIVIEAGTIEDFINVITMSY"
     gene            complement(356..580)
                     /locus_tag="EYO86_02110"
     CDS             complement(356..580)
                     /locus_tag="EYO86_02110"
                     /inference="COORDINATES: protein motif:HMM:PF07750.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41861.1"
                     /translation="MSIIKNYLRQNKVTHIFSSCQWPIGDPQEKDFHFCDTANVVGKP
                     YCQQHCDLAYIDERELKKEKEVQRNRRIAA"
     gene            complement(951..1628)
                     /locus_tag="EYO86_02115"
     CDS             complement(951..1628)
                     /locus_tag="EYO86_02115"
                     /inference="COORDINATES: protein motif:HMM:PF01323.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIC41862.1"
                     /translation="MKTIINCIFSKEHKLIHPITIDIYSDTVCPWCYIGYKKLQLAIN
                     SVSHFKFDLVWRPFQLNPDMPIEGMERQQYLEVKFGGKQKAKDTYQSIYNVGIENNIH
                     FQFGKIVTTPNSFASHKLLAIAHRLNKQSSVVEALFYSYFIEGIDIGNMEQLIIIAKQ
                     HNIYNDKILQYLKSDEDKDNLLAEEIQARELGIKGVPCFIINRKFVLFGAQDKKNFLD
                     IFHKINK"
     gene            complement(1588..>1842)
                     /locus_tag="EYO86_02120"
     CDS             complement(1588..>1842)
                     /locus_tag="EYO86_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016210728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIC41863.1"
                     /translation="SFQQILNHFWKSHDPTQLNRQGLDVGRQYRSAIFYYSKEQHNDA
                     MESKEQTQITFQRAIVTEIMPAQEFYLAEDYHQLYFFKRA"
BASE COUNT          656 a          288 c          251 g          647 t
ORIGIN      
        1 tcataagaca ttgtaataac attaataaaa tcttcaattg ttcccgcttc aataactatt
       61 tctttattca tatatccttc accaagatac aaacctggat tacgaactaa ttttctttct
      121 attgatttat tagtaaaccg aactttggaa tacggtccgg cctgtccaaa actatgctta
      181 tttcctttgt agtcaataaa atcaattttc cctttgaatt ttattttttt taaaattgaa
      241 aaaagcataa aaaattaaaa tgaggagaac tttaaaatat acacaaacaa agtcaacaaa
      301 aggagataaa aaaggctact tactaaaaag tagcctttgt aaaaaataaa aaaaactaag
      361 ctgctattct tcgatttctt tgcacttctt tttctttctt taactctctc tcatcaatat
      421 atgccagatc acaatgttgt tggcaatatg gtttgccaac tacgttagca gtatcacaaa
      481 aatgaaaatc tttttcttgt ggatcgccta taggccactg acaacttgaa aagatgtgag
      541 ttactttgtt ttgccttaaa taattcttaa taattgacat tatgcatcct aataatttct
      601 aaatatatgg aaaatatttt atagttcagt gacggtataa gacggaatat tttaagaatc
      661 aatatacaaa taaaaaaaaa tcttaatcat ctatatatca gtactatttg aaaataacat
      721 acaacatata gcaatgataa tggcttacac acgcctctta aaacgaatcg gttagagaaa
      781 aatcaagatc acgatgtatc caattgcaaa aacaaataaa gatttgagag ttagcttcaa
      841 agtaagctta gatttgggag gttttcgcaa ataaacaaat attataatag tgatccagaa
      901 gagtagcagt atactccaac caattaagaa agcatagtaa aagtcctgac tcacttatta
      961 attttatgaa atatatctaa aaaatttttt ttatcctgag ctccaaataa aacaaatttt
     1021 ctattaatga taaaacaagg aactcccttt attcccaatt cacgtgcttg tatttcttct
     1081 gccaacaaat tatctttatc ttcatcagat tttaaatatt gcaatatttt gtcattataa
     1141 atattatgtt gtttagcaat aataattaat tgttccatat tgccaatatc aataccttca
     1201 ataaagtagg aataaaacaa tgcttccacc acagatgatt gtttattcaa tctatgtgct
     1261 atggccaata gtttgtgtga agcaaatgaa tttggagttg ttacaatttt tccaaattga
     1321 aaatgtatgt tattctctat acccacatta taaatggatt gatacgtatc tttagctttt
     1381 tgtttgcctc caaactttac ttcaaggtat tgttgtcttt ccattccttc aataggcata
     1441 tcaggattta attgaaaagg gcgccatact aaatcaaatt taaaatgaga tactgaatta
     1501 atagctaatt gtaatttttt gtatcctata taacaccagg ggcaaacggt atccgaataa
     1561 atgtcaattg tgatagggtg aatcaattta tgctcttttg aaaaaataca attgatgata
     1621 gtcttcagct aaataaaatt cttgtgcagg cataatttca gtaacaatag ctctttgaaa
     1681 ggttatttgt gtttgttctt ttgattccat agcatcgtta tgttgttcct tggaataata
     1741 gaaaatagct gatcgatatt gtcttcccac atctaatcct tgccgattaa gttgagttgg
     1801 atcatggctt ttccagaaat gattgagtat ttgctgaaaa ga
//
LOCUS       DTVV01000182            1039 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1472353, whole genome shotgun sequence.
ACCESSION   DTVV01000182 DTVV01000000
VERSION     DTVV01000182.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1472353"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            5..550
                     /locus_tag="EYO86_02125"
     CDS             5..550
                     /locus_tag="EYO86_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HIC41864.1"
                     /translation="MLSLIETPLWFSVGEIDETVGAVVPVAEFCGLHELEVQGIADGD
                     VASGMLGYDPIDNNQLTKEEITRCENDSNAQLKLISSTTIDNLPPRKKDIKYTPISLR
                     QEKPYAILWLIKRYPTELSSSQIAKLTGSTKNTVDTIRAGTYREAVLESKSPMDIGLC
                     TYEELEKSLNRSRKQITTPTE"
     gene            complement(601..792)
                     /gene="rpmG"
                     /locus_tag="EYO86_02130"
     CDS             complement(601..792)
                     /gene="rpmG"
                     /locus_tag="EYO86_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIC41865.1"
                     /translation="MAKKSYALKQLLPEENPKTGTKYIVRKPTKGLKASEKLRVKKYD
                     PVLKKHVWFVEKKMPPHSK"
BASE COUNT          357 a          151 c          170 g          361 t
ORIGIN      
        1 caagatgttg tcactgattg aaaccccact ttggttttca gtcggagaga tagatgaaac
       61 agttggtgca gtagtaccag ttgcagaatt ctgtggcctg catgaacttg aggttcaagg
      121 tattgctgat ggagatgttg cttcaggaat gctaggttat gacccaattg ataataatca
      181 gttaactaaa gaagagatta cacgttgcga aaatgattca aatgctcaat tgaaattaat
      241 ttcatcaaca acaattgaca atttacctcc aagaaaaaaa gatataaagt atacacccat
      301 ttctcttcgt caagaaaaac catatgcaat cctttggcta attaagcgat atccaacaga
      361 actgagtagt tcacaaattg caaaacttac tggctcaaca aaaaataccg tggatacaat
      421 tcgtgcaggt acttatcggg aagctgtttt ggaatcaaaa agtccaatgg atattggttt
      481 gtgtacatat gaagaactag aaaaatcact taatcgatct cgaaaacaga taactactcc
      541 aacagagtaa ttttatttat ttcttttcat agaaaagttt ttttaaaaaa tataaataag
      601 ttatttacta tgaggaggca tttttttttc aacaaaccaa acatgttttt ttaaaactgg
      661 atcatatttt ttaacacgta gtttttcaga agcttttaaa ccttttgttg gttttcgaac
      721 tatatacttt gttcctgttt ttggattttc ttcgggaaga agttgtttta aagcatatga
      781 ttttttagcc ataaagtgcc tatatattca aataataatg aaataaagtt ttttatgaaa
      841 aatcagaggg agtatactta atataaggat tataaagacc tttcttttta gaaatttcta
      901 tttgtttatt tctttcttta agtttcaata tcttatcttt tgatattttg ttaatacatt
      961 tggagcagga aattccttta acataatttt ttgataaacg atcttcaaga ccaagaagat
     1021 tgcggcaggc atgacataa
//
LOCUS       DTVV01000183            6497 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1479301, whole genome shotgun sequence.
ACCESSION   DTVV01000183 DTVV01000000
VERSION     DTVV01000183.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1479301"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="EYO86_02135"
     CDS             complement(<1..583)
                     /locus_tag="EYO86_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIC41866.1"
                     /translation="MIDLYTWPTPNGRKISILLEELEAQYNAIPINIGNNEQFQKEFT
                     KISPSNKIPAIFDHETQTSIFESGAIMLYLADKYDKFLPKKSYWITMEWFMFQLTQVG
                     PILGQAHQFLFYHPGQGEFVENKYIDYTKRIYETLNTRLKQRKYLGDEFSIADMATWP
                     WIARFERHKIDLNAYPEVLRWYHSIAKRPRVQKG"
     gene            671..1216
                     /locus_tag="EYO86_02140"
     CDS             671..1216
                     /locus_tag="EYO86_02140"
                     /inference="COORDINATES: protein motif:HMM:PF13181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC41867.1"
                     /translation="MKFFKPIFLIGCFFLNSAAADQLDSRLIELFDKLYIAESKKEIN
                     TLTQDIWKIWDETNDPKIEAYYYRGLEAMRMGDFVMSIDLFTRVIEKNPNFAEGWNKR
                     ATVYYILGQFDKSMIDINKTLLLEPRHFGAMDGMGLIFIQLQQYDKAMKIYDQMLEIF
                     PHSVDTLAKKKLMQEFISQSI"
     gene            complement(1350..2429)
                     /locus_tag="EYO86_02145"
     CDS             complement(1350..2429)
                     /locus_tag="EYO86_02145"
                     /inference="COORDINATES: protein motif:HMM:PF13502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41868.1"
                     /translation="MKKIFFFLLAIPIILLIIFIATYNELGVNKLLANIQKDTNLIIN
                     LEDKGKWKFYPSIGYANIFSAKGIENNVTIDKGKISFKKNYWPLSPLHLELSSPSIHY
                     KGMIFRNSSVKAKYSNQIIDISKFNAKLIDGDINTKGKISVDENKSIQLNGSFNNISL
                     NTILDQANIAKWERVNIKIASPHYNLVTSYKSKESLIANLNGLMEIKGSVFFISTEEE
                     RFGAALLSLLVDKVASLSSISKSISYLLDRFADVPSAITGELQFNNGTISTTDLLLQN
                     NNGRALITAQIDMLSNNINGKINFYEKDSIFVEAVIKGNLQNPEILIGGEVFAEDGST
                     SPRDMKKIFEEGIQSLVDSLLDRND"
     gene            complement(2430..4880)
                     /locus_tag="EYO86_02150"
     CDS             complement(2430..4880)
                     /locus_tag="EYO86_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC41869.1"
                     /translation="MSNSFPSATKVVVIGGGVAGCSVAYHLAKFGWKDTILLERDQLT
                     SGTTWHAAGLIGQLGSSATITKFRKYSLDLYKKLEKETELSTGLKQNGAITVATTSDR
                     LEELKRQATTAQLFDVEANMLNPSEIKKLYPIINTNDIIGGVHMPLDGQADPVGVTNV
                     LAKAARQNGATIVEHCPVKKILTRNNKIVGVETEKGKIDCEYIVLAAGMWSRQIGAEV
                     NVSIPLYPDEHFYILTEGIKDIDRSLPVLRDYNHCLYIKEDAGKLLVGIFEPNAKPAF
                     IKSKLVPNDFSFGELPGDFEHFEPYLMHAIKRIPTLEHAGIRKFFCGPESFTPDTSYL
                     LGETPEIKNFFVCSGFNSIGLASAGGAGRATAEWMINGGVSEDIFCLDISRFEKFHSE
                     TKFITERVTEALGDLYAMHWPYKQNTTSRNQKLLPYHNKLKEAGACFGVGGGFERPMW
                     YARKNQKAEYQYSFGYQNWYESAEFETTHARKYVALFELTPFSKFEMKGTNAHEALQY
                     LCANNIKDTEGNTTYTQMLNSNGGIEADLTVTCIEKKIFRVVSGAAVRSHDKKHILNN
                     LKYDVEFSDITENFACLGLFGPKSRDLLTDLAGDKFNTEQFPFGSGKFLTVEGISIWF
                     QRLSYVGELGWEVYIPIHNSKKIYDTIIEIGKTYQLIHAGAHAMDIMRMEKMYLHWGH
                     DISPEENPYEAGLGFAVSLNKEQDFIGKNALKKIDQNQLTKKLCMFTLEKSEPGKPLL
                     LHEEPIYLEGTIIGQTTSGNYSFNFNKNMSFGYINNYHLLNDLKNKTCEIEVAKKKYK
                     VNLEFQALHDPKNIMIKA"
     gene            complement(4924..>6497)
                     /gene="uvrB"
                     /locus_tag="EYO86_02155"
     CDS             complement(4924..>6497)
                     /gene="uvrB"
                     /locus_tag="EYO86_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIC41870.1"
                     /translation="IGSVDSYSSMSFTLEKNQNINRDIIIRKLVELLYKRRDMDFKRG
                     SFRAKGDILEIFPSHYEDRSWKISMFGDEIESIVEADPLTGEKISKLDHVRIFANSHY
                     VTPKPTIKKALGQIKEDLRKQLIVFESEKKLLERQRLDERTTFDLEMIETTGSCSGIE
                     NYSRYLSGRKPGEPPPTLYEYIPEDSLLFIDESHITCSQIAGMYKGDYSRKSTLSQYG
                     FRLPSCLDNRPLKREEWDAMRPQTIFVSATPGDYELNKTGGIFIEQVIRPTGLVDPPI
                     EIRSTKHQIDNLIDECRNTINNGYRVMITTLTKKMAEDLSEYMHEEGLKVRYLHSDID
                     TLERISIIRDLRLGIFNILIGINLLREGLDIPECALVAILDADKEGYLRSKTSLIQTI
                     GRAARNVDSKVLMYADNETKSMQSAIEETSRRRKKQLQYNKQHNITPLSIKKNIDEII
                     KSTAEQDHVTVDIANTKQLIGKDLDVHIKNLETQMNEFASKLEFEDAAQLRDEINRLK
                     AKQMGLTKKALEYKK"
BASE COUNT         1929 a         1197 c         1073 g         2298 t
ORIGIN      
        1 atcctttttg aactctagga cgtttggcaa tggagtgata ccatcttaaa acctccggat
       61 aagcatttag atcaatttta tgccgttcga atcttgctat ccagggccag gtagccatgt
      121 ccgcgattga gaattcatcc cctaaatatt ttctttgctt taatctggta ttaagtgttt
      181 cgtaaattct ttttgtataa tcaatatatt tattttcaac aaactcacct tgtcccggat
      241 ggtaaaataa aaactgatgc gcttgaccta aaattggtcc cacttgagtt agctgaaaca
      301 taaaccactc cattgttatc cagtaagatt tttttggcaa aaatttatcg tacttgtctg
      361 caagataaag catgatggct ccagattcga aaatacttgt ttgtgtctca tgatcaaaaa
      421 ttgcgggtat tttattgcta ggagaaattt tggtaaattc cttttgaaac tgctcgttgt
      481 ttccgatgtt gatgggaatc gcgttgtatt gggcttctag ctcttcaagc aaaatactaa
      541 ttttgcgacc atttggagta ggccatgtat acaaatcaat cattaattaa tatttttaat
      601 cgtaatatta aaatactcaa ctatattagt attcaattga taataaatat tgagaaatta
      661 ttctgttgct atgaaatttt ttaaaccaat ttttttaata ggttgttttt tcctaaattc
      721 tgctgctgca gaccagcttg attccaggct cattgagttg tttgataaat tgtacattgc
      781 cgaaagtaaa aaagaaataa acacacttac acaagatata tggaagattt gggatgaaac
      841 gaatgatccc aaaattgaag catattatta cagaggactt gaagcaatgc gcatgggtga
      901 ttttgtgatg tcgatagatc tttttactcg tgtaatcgaa aaaaatccta atttcgcaga
      961 agggtggaac aaaagagcca cagtttatta tattttgggc cagtttgata aatcaatgat
     1021 tgatattaac aaaacactgt tattggaacc acgccatttt ggggcaatgg atggaatggg
     1081 cttaattttt attcaacttc aacagtatga taaagctatg aaaatttatg atcaaatgtt
     1141 agaaattttt ccgcatagtg tggacactct ggcaaaaaag aaattaatgc aagaatttat
     1201 ttctcaatca atctagtatc cgaatattaa tattaatatt gataacgtaa aaaagctcac
     1261 aaatgtactt agtacaatat ttgccgaagt aacatcttgt agagcctact ataatatagc
     1321 tgaagacacc agcgatggtg gatagcaaac tagtcatttc tgtcaagcaa actgtctacc
     1381 aaggattgga ttccttcttc gaaaattttt ttcatatccc gaggacttgt acttccatct
     1441 tcggcaaata cctcacctcc aattaatatt tccgggtttt gaaggttgcc cttaataacc
     1501 gcttcaacaa aaatactgtc tttttcataa aaattaattt tgccgttgat attatttgaa
     1561 agcatatcaa tctgtgcggt tatcagggca cgaccattat tgttttggag caacaagtct
     1621 gtagttgaaa tcgttccgtt attaaattgt agttccccgg ttattgcaga gggaacatca
     1681 gcaaatctat caagtaagta actaattgat ttacttatag aggatagact tgccacttta
     1741 tcaaccaata aagatagcag ggctgctcca aatctctctt cctctgttga aataaaaaaa
     1801 acagaacctt taatttccat taaaccattg aggtttgcaa ttaaactctc tttggatttg
     1861 tatgatgtga ctaaattata atgaggggaa gcaattttga tgttaacacg ttcccacttg
     1921 gcaatatttg cttgatctag aattgtattt agtgaaatat tattaaatga accatttaac
     1981 tgaattgatt tgttttcatc tacactgatt tttcccttag tgtttatgtc gccatcaata
     2041 agtttggcgt tgaatttgct gatatcaatt atctggttac tgtattttgc tttaacagaa
     2101 gagtttctaa aaatcattcc tttataatgg atagagggag aagatagttc aagatgcaag
     2161 ggtgataatg gccaatagtt ttttttaaaa ctaattttac ctttatcaat tgtcacatta
     2221 ttttctattc cttttgcaga aaatatattt gcatatccaa tggatggata aaatttccat
     2281 ttccccttat cctctaaatt gattattaaa ttggtgtctt tttgaatatt cgccaataac
     2341 ttgttaacac ccagttcgtt ataagttgct ataaaaatta ttaataaaat tatgggtatc
     2401 gcaagtaaaa aaaaaaatat ttttttcact taagctttaa tcataatatt ttttggatca
     2461 tgtaaagcct gaaattccaa gttaacttta tacttttttt tcgccacctc gatttcacaa
     2521 gttttatttt ttaggtcatt taacaaatga taattgttga tatatccaaa ggacatattt
     2581 ttattgaaat taaatgaata attgccagaa gtggtttgtc caattattgt accttctaaa
     2641 tatatcggct cttcatgcag caacaaaggc tttcccggtt ctgatttttc caacgtaaac
     2701 atacataatt tttttgttaa ttggttttga tcgatctttt ttaaagcatt ttttccaata
     2761 aaatcttgtt ctttgttcag gctgacagcg aatcctaaac cggcctcata gggattttct
     2821 tcaggcgata tatcgtgtcc ccaatgaaga tacatttttt ccatacgcat gatatccatt
     2881 gcatgggcac cagcgtgaat cagttggtat gtttttccaa tttcaataat cgtatcgtag
     2941 atttttttgc tgttatgaat gggaatataa acctcccatc caagttcgcc gacataggaa
     3001 aggcgttgaa accaaatact tattccttca acggttaaaa atttgccact tccaaaagga
     3061 aattgttcag tattaaattt atctcccgct aggtcagtta ataaatccct acttttcggt
     3121 ccaaagaggc caagacaggc gaaattttct gtaatatcac tgaattcaac atcatatttt
     3181 aaattattta aaatatgttt tttgtcgtgc gaacgtactg cagcaccgct aactactcta
     3241 aaaatttttt tttctatgca tgttaccgtc aagtcggcct caatgccgcc atttgaattt
     3301 aacatttgag tgtaagtagt atttccttca gtatctttaa tattattggc gcatagatac
     3361 tgtagtgctt catgagcatt tgttcctttc atttcaaatt ttgaaaaagg tgttaattca
     3421 aagagcgcta catactttct tgcgtgcgtt gtttcaaatt ctgcagattc ataccaattt
     3481 tgataaccaa aactgtattg atattcggct ttttggttct tacgcgcata ccacatcggt
     3541 ctttcaaatc ctccaccaac accaaaacaa gctccggctt cctttaactt attgtgataa
     3601 ggaagtagtt tttgattacg agaagtagtg ttttgtttgt agggccagtg catggcatat
     3661 aggtcgccca gagcctctgt tactctttct gtaataaatt tggtttctga atgaaatttt
     3721 tcaaagcgac taatatccaa acaaaatata tcttcactta ctcccccgtt tatcatccac
     3781 tccgctgtag cccgacccgc tcctccagca ctagcaagac caatgctgtt gaaaccggaa
     3841 caaacaaaga agttttttat ttctggcgtc tctcccagaa gatagcttgt atcgggcgtg
     3901 aaactttctg gcccacaaaa aaatttgcgg atcccagcat gttccaaagt cggtattctt
     3961 ttaatagcat gcattagata gggttcaaaa tgttcgaaat ctccgggcaa ttcaccaaaa
     4021 gaaaaatcat tcggaactaa ttttgatttt ataaaagctg gtttcgcgtt gggttcaaaa
     4081 atgcctacta atagttttcc cgcgtcctct tttatatata agcagtgatt ataatctctc
     4141 agtacaggta acgaacggtc aatgtcctta ataccttctg ttaaaatata aaaatgctca
     4201 tcgggataca aaggaatgct aacattaacc tcagctccaa tttgccttga ccacatacct
     4261 gcagcgagaa caatatattc acaatcaatt tttccttttt cagtttccac accaactatt
     4321 ttattatttc gagtaagaat ttttttgaca gggcagtgct ccacaatggt agccccattt
     4381 tgccgagctg ctttagctag aacgttggtt actcctacgg gatcagcctg tccatccaaa
     4441 ggcatgtgca ctccaccaat aatgtcattt gtatttataa taggatacaa ttttttaatt
     4501 tcagatggat ttaacatatt tgcttcaacg tcaaaaagtt gcgcagttgt tgcctgtcgt
     4561 tttaattctt ctaatcggtc acttgttgtg gcgactgtga tagcgccatt ttgtttcaaa
     4621 cctgttgaaa gttccgtttc cttttccagt tttttataca agtctaaaga atattttcta
     4681 aattttgtaa tcgttgctga ggaacctaat tgacctatca gtccagctgc atgccatgtt
     4741 gttcccgatg tgagctggtc acgttctaaa agaatggtat ctttccaacc aaatttggct
     4801 aaatgatacg caacagaaca accggcaaca cctccaccta tcacgacgac ctttgtggca
     4861 cttggaaaag aattactcat aatcaaagaa ctatgtttat tgaaaatttt gtgaagttag
     4921 aagtcatttt ttgtattcga gcgctttttt tgttaatccc atttgttttg cttttaatcg
     4981 atttatctca tcacgtagct gagcagcatc ttcaaattcc agtttcgaag caaattcatt
     5041 catttgagtt tctaagtttt taatatggac atcaagatcc ttgccaataa gttgcttagt
     5101 gtttgcaata tcaacagtca cgtgatcttg ttctgctgta ctttttataa tttcatcaat
     5161 attctttttt atacttaagg gggtgatatt gtgctgtttg ttgtattgta gttgtttttt
     5221 gcgtcgtcta ctcgtttctt ctatagcgga ttgcatgctt tttgtttcat tgtcagcata
     5281 catgagaact ttactgtcta catttctcgc tgcacgtcct atcgtttgaa ttaaactggt
     5341 ttttgatcgc aaatacccct ctttgtcagc atcgagaatg gctacgaggg cacattctgg
     5401 tatgtctaat ccctctctca acaagtttat tccaataaga atattaaaaa tacccaatcg
     5461 caaatcacgt attattgaaa ttctctcaag cgtatcaata tcagagtgta aatatcgaac
     5521 ctttaatccc tcctcgtgca tgtattcgct taagtcttcc gccatttttt ttgttaatgt
     5581 agtaatcatt actctatagc cattgtttat agtattacgg cattcatcaa taagattatc
     5641 aatttgatgt tttgtagaac gtatttcaat agggggatct acaagtccgg ttggacgaat
     5701 tacttgttca ataaaaatac cccctgtttt atttaattca taatcaccgg gagttgcgct
     5761 tacaaatatt gtttgaggtc gcattgcatc ccattcctct cttttaaggg gacgattatc
     5821 aaggcagctt ggcaatctaa aaccatattg agacaaggtt gattttcttg agtaatcacc
     5881 tttatacatc ccagctatct ggctacaggt aatatgactt tcatctataa acaatagcga
     5941 gtcctcggga atgtattcat ataacgtagg aggaggctcc ccaggtttcc tacctgacag
     6001 gtaacgggaa taattttcaa taccgctaca actaccagtt gtttcaatca tttccagatc
     6061 aaacgttgtt cgttcatcta ggcgttgtct ctcaagtaat tttttctctg attcaaatac
     6121 tattaattgt tttcgcaaat cttcttttat ttggccaaga gcttttttta ttgttggttt
     6181 tggggtgacg taatgagaat ttgcaaatat gcgtacatga tccagttttg aaattttttc
     6241 tcctgttagt ggatcagcct caacaataga ttcaatctca tcaccaaaca tagaaatttt
     6301 ccaggaacga tcttcatagt gtgatggaaa aatttctaat atatctccct tagcgcgaaa
     6361 acttccgcgc ttaaaatcca tatcacgcct tttgtataat agttctacca attttcttat
     6421 gatgatatca cgattaatgt tttgattttt ctctaaagta aaagacatgg aagaataaga
     6481 atctactgaa ccaattc
//
LOCUS       DTVV01000184            2020 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1481458, whole genome shotgun sequence.
ACCESSION   DTVV01000184 DTVV01000000
VERSION     DTVV01000184.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1481458"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            6..89
                     /locus_tag="EYO86_02160"
     tRNA            6..89
                     /locus_tag="EYO86_02160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:39..41,aa:Leu,seq:caa)
     gene            293..883
                     /locus_tag="EYO86_02165"
     CDS             293..883
                     /locus_tag="EYO86_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIC41871.1"
                     /translation="MLRNLYNWTLDKASHKKASWFLVLISFAESSFFPIPPDILLIPM
                     ILAKRLQAWFYALICTLSSVAGGICGYTIGYLFYTSIGSIIINAYGLTNSFDVFQCYY
                     NQYGIWIVLGAGFTPFPFKVITIASGMFNLNIVLFVIIATIARGLRFYLIATLLFIYG
                     NVIKNLIDKYFNLMVTLFFILLVGFVFLLKLFSFSC"
     gene            894..1388
                     /locus_tag="EYO86_02170"
     CDS             894..1388
                     /locus_tag="EYO86_02170"
                     /inference="COORDINATES: protein motif:HMM:PF02600.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIC41872.1"
                     /translation="MILRYWFWITSILSLLAISSALIAEYSFDLEPCSMCLKQRHPYY
                     FILAIFILFGMILPLRRIWFYLGVQLACIYGLFYSIWHVGIEKKILPGPTSCSGELNF
                     SNSAENLKEQIMAKAVINCEDVIWSFIGVSAATINTLLLLLIFIVNAIYLKKYYASKK
                     NAAS"
     gene            1360..1929
                     /locus_tag="EYO86_02175"
     CDS             1360..1929
                     /locus_tag="EYO86_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HIC41873.1"
                     /translation="MPQKKTQRHKTKYKDQKNTDRAILEEIIRVDHAGEFGATRIYDG
                     QIAVFGTKSKIGKTIKHMADQEQEHIETFEKLILNHRVRPTALLPVWNVAGYFLGAAT
                     AMMGEKAAMACTVAVEKVIGEHYHKQLSLLKGDHKKLKATIKKFAQDELEHHDIGIAH
                     DAEKTPGYTILTKFIEMGCKTAIAISKKI"
     gene            complement(1926..>2020)
                     /locus_tag="EYO86_02180"
     CDS             complement(1926..>2020)
                     /locus_tag="EYO86_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIC41874.1"
                     /translation="QLQNNGRNFPPNFLHETWRDYLYWDTELQS"
BASE COUNT          616 a          318 c          382 g          704 t
ORIGIN      
        1 tatgtgggga gctgatggaa tggtagacat gtgggtctca aaaacccatg ccgcaaggcg
       61 tgagagttcg attctctcgc tccctaccaa gaccgcgtta gacaaaaatt ttgttgcgtc
      121 tagtaaggga aagtctatag tttttgtgtc gtctttgtgt cgtcctaaaa cgataaaagg
      181 ttttagaaat gaaggtgagc tttatttttt ttaatgcgtt tagattcaaa atccggtcac
      241 tccggtggtg tgggttcgat tccctctact cctaccaaat aatcaaatga atattcttcg
      301 taacctttac aattggacat tagataaagc tagtcacaaa aaagcaagtt ggtttttagt
      361 tctgatatct tttgctgaga gttctttttt ccccattcca cctgacattt tattgatacc
      421 gatgatttta gcaaaacgct tgcaagcttg gttttatgca ttgatttgca cgttgtcttc
      481 agtagccggt ggtatatgtg gctatacgat tggatattta ttttatacaa gcattggttc
      541 tataattatt aatgcgtatg gcttgacgaa ctcatttgat gtttttcaat gttattataa
      601 tcaatacggc atatggatag ttttaggagc gggttttaca ccttttcctt ttaaagttat
      661 tacaattgca agtggtatgt ttaatttaaa tattgtactt tttgttatta ttgcaacaat
      721 tgcaagagga ctacgctttt atttaattgc aacattgcta tttatatacg gtaatgtgat
      781 caaaaattta attgataagt attttaatct tatggtaact ttatttttta tattacttgt
      841 aggatttgta ttcttgttaa aattattttc attttcatgt taaaatcatt ataatgatat
      901 tacgttattg gttttggatt actagtattc taagtttact agcaattagt agtgcattaa
      961 ttgcggaata ttcttttgat cttgagcctt gctcaatgtg cttgaaacaa cgtcatccct
     1021 attactttat tcttgcaatc tttattttgt ttggaatgat tcttcctttg cgtcgtattt
     1081 ggttttattt aggtgttcag ctagcttgta tttatggatt attttattca atttggcatg
     1141 ttggtattga gaaaaaaatc cttccaggcc caactagttg ctcaggtgag cttaacttta
     1201 gtaactcagc tgaaaatcta aaagagcaaa tcatggcaaa agcagtgatc aattgtgaag
     1261 atgtgatttg gagttttatt ggtgtttccg ccgctacaat aaacaccctt cttcttcttt
     1321 tgatttttat tgtaaacgct atatatttaa aaaaatacta tgcctcaaaa aaaaacgcag
     1381 cgtcataaaa ctaaatacaa ggatcaaaaa aatacagatc gagctattct agaagaaatt
     1441 attcgtgttg atcatgcagg ggaattcggg gctactcgta tttatgatgg tcaaattgct
     1501 gtatttggta caaaatccaa aattggtaaa acgataaagc acatggctga tcaggaacag
     1561 gaacatattg aaacatttga aaagctcatt cttaatcatc gtgttcgccc tacagcacta
     1621 ttgcctgtat ggaatgttgc aggttatttt ttaggagcgg caacagctat gatgggggaa
     1681 aaagcggcaa tggcgtgtac agtagcagtg gaaaaagtta ttggggagca ttaccacaaa
     1741 caactttctt tattaaaagg ggatcataaa aaactaaagg ctactataaa aaagtttgca
     1801 caagatgaat tagagcatca cgatattggt attgcccatg acgcagaaaa aacccctggc
     1861 tacaccatac taactaagtt tattgagatg ggatgcaaga cagccatagc aatatcaaaa
     1921 aaaatctaac tttgtaactc cgtatcccag tacaaataat cccgccaggt ttcatgtaaa
     1981 aagttgggag ggaaattacg accattattt tgcaactgaa
//
LOCUS       DTVV01000185            3474 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1481724, whole genome shotgun sequence.
ACCESSION   DTVV01000185 DTVV01000000
VERSION     DTVV01000185.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1481724"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="EYO86_02185"
     CDS             <1..241
                     /locus_tag="EYO86_02185"
                     /inference="COORDINATES: protein motif:HMM:PF03819.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIC41875.1"
                     /translation="EIKKKNKKRIQEELGDMLFASLDVARKLQLNPEISLAKSNEKFS
                     KRWGRLEQFIINDKKEFNDLNLKDFNFYWNKAKIN"
     gene            complement(238..831)
                     /locus_tag="EYO86_02190"
     CDS             complement(238..831)
                     /locus_tag="EYO86_02190"
                     /inference="COORDINATES: protein motif:HMM:PF09335.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41876.1"
                     /translation="MTEFYVFLDFLNIYIDTNYNFSILIFFVFLLFYNTFSIPGNLIF
                     VVSAGYFFGIYIGYIISILSIVLGSLIFFTVSKLFLKKLFTNIYDKYSTKINKYISNS
                     SIEYLILFRMMPGPPLMMQNVCLSILKINSIKFILTSFIGFSPIIFVAVYFGSKIKSF
                     ESIKNITMNSILSRDFLIFVVLMIFFLSLRIIFKKKS"
     gene            complement(831..1598)
                     /locus_tag="EYO86_02195"
     CDS             complement(831..1598)
                     /locus_tag="EYO86_02195"
                     /inference="COORDINATES: protein motif:HMM:PF01061.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41877.1"
                     /translation="MSNISYFAIKTIFIYEVKDFFKEFQFNIVAPLINTLLFVIILST
                     IESYYSIPSEGGSYLNFLVPGIIIMVVMQASFNHLSEVIIAMKQIGSFNDYLMSPISR
                     IEIFFSLILSSIVVCVFIGMINLTVLTFFTNFQDINYFRLFYYLVISIIIFSSIGAVT
                     GFLSFTWDAQSTVSNFVIVPISLLSGTFFSIDSLDNQWKIIFEYNPFYHLVSGFRGAF
                     IENSILNANTDILVTMIAILTLLISLFIFNKGYKVIY"
     gene            complement(1602..2774)
                     /locus_tag="EYO86_02200"
     CDS             complement(1602..2774)
                     /locus_tag="EYO86_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIC41878.1"
                     /translation="MPILNSINQMQDEMKTWRQELHRIPEIGLEEYKTSSYIKEKLKK
                     WGIEYKDGYSNTGIVAWVRGTKGNSDKSIGLRADFDALPMTERNNFKHKSTNKGMMHA
                     CGHDGHTSMLLGAAKYLSENPDFDGTVHFIFQPGEEGFGGGEKMIQDGLFQDFKIDEV
                     YALHNWPGLKLGQFGVSTGPMMAAVDEFDIVVKGKGGHAAMPDLAVDPVIIASQIINS
                     LQTIISRIINPVDKALISVTKIHGGSAYNVIDDEVKLGGTVRTFKNETRDLIEKKIND
                     TAKGIAIANGGEAEIQYYRKYPATINSKNESIFAGNVAKELVGEKNVITEVEPSMGGE
                     DFSYLLNEKPGSYLYIGQNDNYHNAHLHTTQYDFNDNLLPLGVNYWIELVKKFFQK"
     gene            complement(2774..3406)
                     /locus_tag="EYO86_02205"
     CDS             complement(2774..3406)
                     /locus_tag="EYO86_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41879.1"
                     /translation="MLIRFFMRFENIAIIFSLILIFTVACTSLKQIDTANSCILFDSK
                     KGWYKSTKKSFDKWGTPIALQLAIINQESSFNQFAKPKRKRLFGIIPGSRPSTAFGYA
                     QVINPTWNWYKSRTGNKNASRANFQDVTDFIGWYSEQSKKMLGIPKNDFYNQYLAYHE
                     GQSGWKNKSFNSKKWLLGVAKKVETNANKYNNQLKDCENKLNAKGLFGIF"
BASE COUNT         1304 a          535 c          448 g         1187 t
ORIGIN      
        1 agaaatcaag aaaaaaaata aaaaaagaat acaagaagaa ttaggtgata tgttatttgc
       61 ttcacttgac gttgcaagaa aattacagct taatcccgaa atatctctag ctaaatcaaa
      121 tgaaaaattc tctaaaagat gggggagatt ggagcaattt attatcaatg ataaaaaaga
      181 atttaatgat ttaaacttaa aagattttaa tttttactgg aataaagcaa aaataaatta
      241 acttttcttt ttgaaaataa ttcttaagga taagaaaaaa atcataagga caacaaaaat
      301 taaaaaatct ctggataata tactattcat agttatattt ttaatcgatt caaaagactt
      361 gattttactt ccaaaataaa cagcaacaaa tataattggg gaaaaaccaa taaaggaagt
      421 taatataaac ttaatagaat taatttttag tattgataaa cacacatttt gcatcattaa
      481 tgggggtcct ggcatcattc gaaacaatat gagatattca atagaagaat ttgaaatata
      541 tttgttaatc tttgtggagt atttatcgta aatatttgta aataattttt ttaagaataa
      601 ttttgaaact gtaaaaaata ttaaactgcc caaaacaatt gataaaatag atataatata
      661 gcctatgtaa attccaaaaa aataaccagc agaaacaaca aatattaaat ttcctggtat
      721 agaaaaagta ttataaaaaa gaagaaaaac aaaaaatatt agaattgaaa aattataatt
      781 agtgtcaata tagatattaa gaaaatctaa aaatacataa aattcagtca ttaatatata
      841 actttataac ctttattaaa tatgaaaagt gaaattaata aagtaagaat tgctatcatc
      901 gtaacaagta tatctgtatt tgcatttaga atactatttt caataaaagc tcctctaaaa
      961 ccacttacta aatgataaaa ggggttatat tcaaaaatta ttttccattg attgtctaat
     1021 gaatcaatcg aaaaaaatgt acctgataaa agactaatag gaacaataac aaaatttgaa
     1081 actgtacttt gggcatccca agtaaaagac aaaaaaccag tgacagctcc aattgatgaa
     1141 aatataataa tcgaaataac taaataataa aataatctaa aataatttat gtcttgaaaa
     1201 tttgtaaaga aagttaatac agtcaaattt atcattccaa taaaaacaca aacaacaata
     1261 gaggataaaa taagggaaaa gaatatttca atacgggaaa tgggtgacat taaataatca
     1321 ttaaacgatc ctatttgctt catggctata attacttctg ataaatgatt gaaacttgct
     1381 tgcatcacaa ccattataat gattcccgga actaaaaaat ttaaatacga tcctccttca
     1441 gatggaatac tataataact ttcaatagtg gataaaatta taacaaataa taaagtgttg
     1501 attaatggtg ctacaatatt gaattggaat tctttaaaaa aatcttttac ctcatagata
     1561 aagattgttt tgattgcaaa gtaactaatg ttactcattg tttatttttg aaaaaatttt
     1621 ttaactaatt ctatccaata atttactcct agaggtaata gattgtcatt aaaatcatat
     1681 tgagtagtgt gtaaatgagc attatgataa ttgtcatttt gaccaatata cagataagag
     1741 cctggttttt catttaataa atatgaaaaa tcttcaccgc ccattgaagg ttctacttct
     1801 gttataacat ttttttcacc cactaattct tttgctacat tgccagcaaa aatactttca
     1861 tttttagagt taatggtagc cggatatttt ctataatatt gaatttcagc ttccccacca
     1921 tttgctatag cgataccttt agccgtatca tttatttttt tttcaattaa atctcttgtc
     1981 tcatttttaa aagtcctaac agtgcctccg agtttaacct catcatcaat tacattatag
     2041 gcgcttcctc catgtatttt cgttacgctt attaaagcct tatcaacagg gtttatgatt
     2101 ctactaataa tagtttgaag agagttaata atttgagagg ctattatcac tggatctact
     2161 gcaagatcag gcattgctgc atggcctccc ttccccttga caactatatc aaattcatca
     2221 actgcagcca tcatcggtcc ggttgaaaca ccaaattgtc ctagtttcaa ccctggccaa
     2281 ttatgaagtg catagacttc atcaatctta aaatcttgaa acagtccatc ctgaatcatt
     2341 ttttctccac caccaaaacc ctcttctcct ggttgaaaaa tgaaatgaac agttccatca
     2401 aagtcagggt tttcactaag atattttgct gctcccaata gcatcgaggt atgaccgtca
     2461 tgaccgcatg catgcatcat tcctttgttt gtagatttat gtttaaaatt atttctttcg
     2521 gtcattggaa gagcgtcaaa atcagctctt aatccaattg atttatcact gtttccctta
     2581 gtacccctta cccaagcaac aataccggta ttagagtaac catctttata ttctataccc
     2641 cactttttga gtttttcttt tatatatgaa gatgttttgt attcttctaa gccaatttcg
     2701 ggaattcgat gaagttcttg cctccaagtt ttcatctcat cttgcatttg attaatacta
     2761 tttaaaattg gcatcaaaat attccaaata atccttttgc atttaattta ttttcacagt
     2821 cttttaattg attgttatac ttatttgcat tggtttcaac ctttttagca acgcccaata
     2881 accatttttt tgagttgaaa gatttatttt tccaaccact ctgtccctca tgataagcta
     2941 aatactgatt ataaaaatca ttttttggaa tccccagcat tttcttagat tgttcagaat
     3001 accaaccaat aaaatctgtt acatcttgaa aatttgctcg agatgcattt ttatttcctg
     3061 ttcgtgattt ataccaattc caagttgggt ttataacttg agcataacca aatgcagttg
     3121 aaggtctact acctggaata atgccaaaca atcttttacg tttaggttta gcaaattgat
     3181 taaaggaaga ttcttgattt ataatagcta attgaagcgc tataggtgtt ccccacttat
     3241 cgaaactttt ttttgtcgat ttataccaac cttttttact atcaaataaa atgcaactat
     3301 tagcagtatc aatctgtttc aaggaagtgc atgcaactgt aaaaattaaa attagagaaa
     3361 aaataattgc aatattttca aaacgcatga agaatctaat taacataatt ttttaactgt
     3421 ataaccataa atatggcaaa aaaagattgt aacctattga atatgcaata agag
//
LOCUS       DTVV01000186            1640 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1484123, whole genome shotgun sequence.
ACCESSION   DTVV01000186 DTVV01000000
VERSION     DTVV01000186.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1484123"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="EYO86_02210"
     CDS             complement(<1..271)
                     /locus_tag="EYO86_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIC41880.1"
                     /translation="MQNSFDEKILDEANDWIKANQKVVLATVIQTWGSSPRPTGSRMV
                     VNENGDFSGSVSGGCVESAVVRECVGIIKENKPFKKIEFKVSNESA"
     gene            complement(306..1148)
                     /locus_tag="EYO86_02215"
     CDS             complement(306..1148)
                     /locus_tag="EYO86_02215"
                     /inference="COORDINATES: protein motif:HMM:PF12867.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41881.1"
                     /translation="MNNDEEVKVYWQPGCSSCLKTKEFLMDNGIPFKSVNVLEDKTGF
                     EDLQRFGVKMVPIVAKGDAWANGAVFRDVAEVAGFDYQAHKMLEPSIIKDKILQINSS
                     ASKYLEQIPDEELDTLLPGRPRSYRQLVYHIFNIQDVFLKRVEDNAPYTYESLLSILP
                     KEMKTKKDLIDYGIEVGNRFSNWWSRAGTSTEFKQPGNVYYGEVILHEVLERTAWHSG
                     QHVRQVALMLKEKLNIEPKPPLDNTIFHGLPMPKNIWDNERSFSEKSYSGQALSSVED
                     FKNL"
     gene            complement(1181..>1640)
                     /locus_tag="EYO86_02220"
     CDS             complement(1181..>1640)
                     /locus_tag="EYO86_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HIC41882.1"
                     /translation="IANNDPAADFPSACLALNAIIHTSKRKIQADKFFKGMFETDLKK
                     GELIEAIEFKVPDKSSYVKFPNPASRYAIVGIYVAKLKKDVRVAVTGVESCVFRCKKL
                     ESVLSSDFSPSSIDKISISSKGFNADIHASAEYRANLIVVLAKKAVETSK"
BASE COUNT          496 a          325 c          222 g          597 t
ORIGIN      
        1 aagcgctttc attcgaaact ttaaattcaa tttttttaaa tggtttgttt tcttttataa
       61 ttcccacaca ttctctgaca actgctgact ccacacatcc accggaaaca gatccagaaa
      121 aatctccatt ttcattgaca accattctgc ttccggttgg tcttggagat gatccccatg
      181 tttgaattac cgttgcaagg actacttttt gattagcttt tatccaatcg ttagcttcat
      241 ctagaatctt ttcatcaaaa gaattttgca tacaataact ataacagaaa gtttgttttt
      301 atggattata aatttttaaa atcctcaacg cttgataaag cctgacctga ataagatttt
      361 tctgaaaatg atctctcgtt atcccatatg ttttttggca ttggtaaacc atgaaaaatc
      421 gtattatcta atggaggttt cggctctata tttaattttt cttttaacat caaagctacc
      481 tgcctcacat gttgtccact gtgccatgct gttctttcta agacttcgtg caaaattact
      541 tctccataat aaacattgcc tggctgctta aattcagtgc tggtgccagc tctgctccac
      601 caattagaaa acctgtttcc aacttctata ccataatcta tcaaatcttt ttttgttttc
      661 atttcctttg gtaatattga aagtaatgac tcgtaagtat aaggagcgtt atcttcgact
      721 ctttttagaa aaacatcttg aatattaaaa atatggtaaa ctaattgtct ataagatctt
      781 ggacgacccg gtagcaaagt atcaagttct tcatcaggta tttgctctaa gtactttgat
      841 gcagacgaat taatttgcaa aattttgtct tttataatcg atggctcgag cattttatgg
      901 gcttgataat caaaaccagc aacctcggct acatccctaa atacggctcc gttagcccaa
      961 gcatctcctt tagcaacaat tggaaccatt ttgactccaa atctttgaag atcttcaaat
     1021 cctgttttat cctctaaaac atttactgac ttaaatggaa taccattatc cattaaaaac
     1081 tccttagtct ttaaacaact tgaacaacca ggttgccagt agacttttac ttcttcatca
     1141 ttgttcataa aaattatttt aaaccttttt ttagtacaaa ctacttagag gtctcaacag
     1201 cttttttagc taataccaca attaaattag ctctatattc agctgaagca tggatatctg
     1261 cattaaatcc cttacttgaa atactaatct tgtcaattga tgagggtgaa aaatcgcttg
     1321 ataagacact ttcaagtttt ttacatctaa atacacagct ttcaacaccg gttacagcaa
     1381 ctctgacatc tttttttaat tttgcaacat aaatgcccac tatagcatat cttgaagcag
     1441 gatttggaaa ttttacgtag ctagatttat ctggaacttt aaattcaatc gcttctatta
     1501 attcaccttt ttttaaatct gtttcaaaca ttcctttaaa aaatttatct gcttgaattt
     1561 ttcttttact tgtatgtatt attgcattta aagccaaaca agccgaaggg aaatctgctg
     1621 cagggtcatt atttgctatt
//
LOCUS       DTVV01000187            3493 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1484151, whole genome shotgun sequence.
ACCESSION   DTVV01000187 DTVV01000000
VERSION     DTVV01000187.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1484151"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(164..1015)
                     /gene="prmC"
                     /locus_tag="EYO86_02225"
     CDS             complement(164..1015)
                     /gene="prmC"
                     /locus_tag="EYO86_02225"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIC41883.1"
                     /translation="MNSFLQDNLNLLQNKNIPCPELELRVLLNKTSVIDKEIIFSNFE
                     LNSIDLKAFHQAIKRRANNEPISKIFNEKKFWKYSFFVNKNVLDPRPETELVIEKVIK
                     YFPKKDEKLKILDICTGSGCLAISLAREYRNAKITATDISSDALKVAQINANKLCSDK
                     EIQFVKCNLIKNIDTYDIIVSNPPYLSDLEYKKTTKEIQSYEPKIALIGGIDGLKFYR
                     NIANFLTEILSSTSIVFIEIGNNQAKEVMKIFKSKKIKCLELAKDIQQLNRLLILQKN
                     QYKINVD"
     gene            complement(1008..2084)
                     /locus_tag="EYO86_02230"
     CDS             complement(1008..2084)
                     /locus_tag="EYO86_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIC41884.1"
                     /translation="MKDFNFQLDAILKKYKEIEKQLSNQAVLERNKLIELNKEYSELT
                     SIVEVINRFNGHKQDLLNLNELLKDADSSIREMAEAEIKEKNDQLKLIEVELLKSLIP
                     KDVNDEKNSILEIRAGTGGVEASLFAADLFTMYQKYSELNSWKFDILSISETGLKGIK
                     EAICNISGRNVFSKMKFESGVHRVQRVPATENSGRVHTSAATVAVLPEAEEIDVKIEE
                     KDLRIDVFRSSGPGGQSVNTTDSAVRITHIPTGLVVSQQDEKSQHKNKAKALKILRSR
                     VLDKEIQLKDQERAKDRKSQVGTGDRSERIRTYNFPQGRVSDHRINLTLYNLSEILEG
                     QLDELIIPLCAEAESKKLSELNNE"
     gene            complement(2081..3316)
                     /locus_tag="EYO86_02235"
     CDS             complement(2081..3316)
                     /locus_tag="EYO86_02235"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIC41885.1"
                     /translation="MKIQSVRGTHDLYAQQLEKFKFIIEIVNTYAHIYDFNEIITPIF
                     EFTELFLKPLGEHSDVVLKEMYTFDDRNDSSLTLRPEYTTPIIRAAISNNLLERLPTK
                     LYGIGPMFRRERPQKGRFRQFNQINFEILGTNDMAADVELILLADDILKKIFLNKKIK
                     LVINSLGDKDTLKKFKHYLSVYFKKNKSKLSIDSLNRIESNPLRILDSKNKNDNDVSQ
                     EAPKISDFYSEEAKNKFDDIKNLLTNRSVEFEVNHSLVRGLDYYCHTVFEFKSNELGS
                     QDTLIGGGRYDGLTKTLGGPDIPGVGWAGGVERIMMLIEKDIKVQQKIHLVVMKEDFR
                     SYGLDILNKLRKNNIPVFFDYKYNLKKSLSFANNVNARYAIIIGEEEVKKHSYTIKDL
                     KKNTQKTILLDPLISLLKL"
     gene            complement(3313..>3493)
                     /locus_tag="EYO86_02240"
     CDS             complement(3313..>3493)
                     /locus_tag="EYO86_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /protein_id="HIC41886.1"
                     /translation="VNGPGEAKMTQIGITGGGNDTHMVYINGEKNHRIKNEDLPTYLE
                     KIIRNQASEQSNSNT"
BASE COUNT         1046 a          578 c          449 g         1420 t
ORIGIN      
        1 tagttatttc gtttaaaatt cgaccttcta tttgatctaa acgttactcg cgcatttttt
       61 tttatgaaca tatactactg ttaaaattaa tcagaaaact ttaaaattac caatttagtt
      121 ttcaactata taatctgtct acactagtat ataaattcat taattaatca acatttattt
      181 tgtattgatt tttttgtaat attaataaac gatttaattg ttgaatatct tttgctaatt
      241 ctagacattt aatttttttg ctcttaaaaa ttttcatcac ctctttagcc tgattgttac
      301 cgatttctat aaatacgata gaggttgaag ataaaatttc agttaaaaag tttgcaatat
      361 ttctataaaa tttcaagcca tcaattcccc cgattaaggc tatttttggt tcatatgatt
      421 gaatttcttt tgttgttttt ttatactcta aatctgatag gtagggtgga tttgaaacta
      481 taatatcata tgtatctata ttttttatca agttacattt aacaaattga atttctttat
      541 cgctacacag tttatttgca tttatttgag ctacttttaa agcatcggaa gaaatatcag
      601 ttgctgttat ttttgcattt ctgtattctc tagccagaga aattgccagg catcctgaac
      661 ctgtgcatat atctaaaatt tttaattttt catctttttt tggaaaatat ttgatgacct
      721 tttctatgac taattctgtc tctggtctgg gatcaagcac gttcttatta acaaaaaaac
      781 tatatttcca aaattttttt tcattaaata tcttagatat aggttcgtta tttgctcttc
      841 tttttattgc ttggtgaaaa gctttaaggt caatggaatt taattcaaaa ttactaaata
      901 taatttcttt atctataact gaagttttat ttaacaaaac tcgtagttct aattctggac
      961 atggaatatt tttgttttgt aataagttaa gattgtcctg taaaaaacta ttcattatta
     1021 agttctgata atttttttga ctctgcttcc gcacataatg gaataattaa ttcgtctaac
     1081 tgtccttcaa gtatttcact taaattgtac aatgtaagat ttattcgatg atcggaaacg
     1141 cggccctgag gaaaattata ggttctaatt ctttcagatc tgtcaccggt acctacttgt
     1201 gacttcctat cctttgctct ttcttggtct tttaattgaa tttctttatc taaaacacgc
     1261 gatcttagaa tttttaaagc ttttgcttta tttttatgtt gtgatttctc atcttgctga
     1321 gatacaacta gacctgttgg aatatgagta attctcacgg cactgtccgt tgtgttaact
     1381 gattgacctc ctggaccact tgaacgaaaa acatctatgc gcaaatcttt ctcttcgatt
     1441 tttacatcaa tttcttctgc ttcaggtaaa actgctactg ttgctgcaga tgtatggact
     1501 cttccactat tttctgtagc tggcacacgt tgaacacgat gcacgcctga ttcaaatttc
     1561 attttcgaga atacatttct tccagagata ttgcatatgg cttccttaat acctttcagg
     1621 ccggtctctg aaattgataa aatatcaaat ttccaggaat ttaattccga atatttttgg
     1681 tacatagtga ataaatcagc agcaaataag gatgcttcga caccacctgt gccagctctg
     1741 atttctaata ttgaattttt ttcatcatta acatcttttg gaattaaaga tttaagtaat
     1801 tcgacctcta ttaactttag ttggtcgttt ttctccttga tctctgcttc tgccatttct
     1861 ctaattgatg aatcagcatc cttaagtagt tcatttaaat ttaataaatc ttgtttatgg
     1921 ccattaaacc tatttattac ctcaactatt gatgttaatt cagagtattc cttgttcaat
     1981 tcaataagtt tatttctttc caaaacagct tggttagaca attgtttttc tatttcttta
     2041 tatttcttta aaatagcatc aagctggaaa ttaaaatcct tcataatttt aacaatgaaa
     2101 ttaatgggtc aagtaaaata gttttttgtg tatttttttt taaatctttt attgtataag
     2161 aatgtttttt aacttcttct tcaccaataa taattgcata tctggcattc acattattag
     2221 caaaggataa agattttttt aaattatatt tataatcaaa aaaaacaggg atattatttt
     2281 ttctcaattt atttaatata tctaaaccat aagatctaaa atcttccttc atcacaacca
     2341 gatgaatttt ttgctgaact ttaatatctt tttcaattaa catcataatt ctctcaacac
     2401 ctccagccca accaactccc ggtatatctg ggcctcccaa ggttttagtt aatccgtcat
     2461 aacgtccacc tccgatgagc gtatcttgtg accctaattc atttgattta aattcaaata
     2521 cagtatgaca atagtaatca agccctctaa caagactatg gtttacctca aattctactg
     2581 aacgatttgt taataaattt tttatatcat caaatttatt tttggcctct tctgagtaaa
     2641 aatccgatat tttaggagct tcttgactaa catcattatc atttttattt ttagaatcta
     2701 gtattcttag ggggtttgat tcaattctat tcagagaatc tatagataat ttagatttat
     2761 tttttttaaa gtaaacactt aaataatgct taaatttttt taaagtatcc ttatctccta
     2821 atgaattaat tactaattta atttttttat taagaaaaat ttttttcaaa atatcatctg
     2881 ccaataaaat taattctaca tcggctgcca tgtcattcgt tcccaatatt tcaaaattta
     2941 tttgattaaa ttgtctaaat ctaccttttt gaggtctttc tcgtctaaac attggtccaa
     3001 ttccatatag tttggttggc aacctttcta aaagattatt tgaaatagct gccctaatta
     3061 tgggcgttgt atattcaggt cttaaggtta aagatgaatc gttacgatca tcaaatgtat
     3121 acatttcttt taatactaca tcactatgct cacccaaagg ttttagaaaa agctcagtaa
     3181 actcaaagat aggtgttatg atttcattaa aatcatatat atgtgcgtat gtattaacaa
     3241 tttctataat aaatttgaat ttttctagct gttgggcata aaggtcgtgc gttcctctta
     3301 ctgattgaat cttcatgtgt tgctattcga ctgttctgaa gcctgatttc taattatctt
     3361 ttcaagatat gttggcagat cttcattctt tattcgatga tttttttcgc cattgatata
     3421 aaccatgtga gtgtcatttc cacctcctgt aatgccgatt tgcgtcatct tagcctctcc
     3481 tggtccatta aca
//
LOCUS       DTVV01000188            1640 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1486657, whole genome shotgun sequence.
ACCESSION   DTVV01000188 DTVV01000000
VERSION     DTVV01000188.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1486657"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            371..>1640
                     /gene="glpK"
                     /locus_tag="EYO86_02245"
     CDS             371..>1640
                     /gene="glpK"
                     /locus_tag="EYO86_02245"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIC41887.1"
                     /translation="MKKFIISIDQGTTSSRAILFNLKGEPVYVAQTEFTQYFPKDGWV
                     EHDPNEIWGTTLKVLKDVIKKSKKLKGKVLTIGITNQRETTILWDKITGKPVYNAIVW
                     QDRRASNYCKKLIKQKKETIIYNKTGLLIDAYFSATKIKWIIDNVAKARELIKKKRLL
                     FGTVDSFLLWKLTNGVEHATDATNASRTMLFNISTNQWDHQILKMLNIPKNILPTVKD
                     CSADFGHTDPLLTGKSYPITGIVGDQQSATIGQCCFEPGSLKSTYGTGAFVLLNTGSK
                     KIYSKNRLLTTIGYRINGKTTYALEGSIFIAGAGVQWLRDKIKFIKKASDTEKIIRSL
                     KSNKGIYLVPAFTGLGAPYWAVNARGVLSGLTRDTGPSEIVRATVESVAYQTFDLFEA
                     MKNDGLRPKIIKVDGGMVKNSWFSQFLSDIL"
BASE COUNT          654 a          207 c          276 g          503 t
ORIGIN      
        1 acactacctc gcactagctc acactaacaa actcatcaaa gtcttatcta agaaaaagtt
       61 attacagttg ttggtttatt ccagtaagaa aaaaacaagt gtagttaatt tgaagttaaa
      121 tttgtttagg actcttgacg caactctaga ggtaagtagt atcaatgtta aatgaaagga
      181 ttttttgtta gctaattgat ttgtaatcag aatatatttc taatactgga cttagaaaat
      241 ttaaaacaaa caaagagtag atttatcagc aaaaggtaat gttatataga agatctataa
      301 taaaaattat aggcaatgaa actgctggcg tatattaatt ttattgtttt gattcgaagg
      361 atattaaatt atgaaaaaat ttataatttc tatagatcaa ggaactactt caagcagagc
      421 aatcttattt aatttaaaag gtgagccagt ttatgtagca caaactgaat tcacacaata
      481 ttttccaaaa gatggttggg ttgaacatga cccaaatgaa atttggggta caacattaaa
      541 agtattaaaa gatgtaatta aaaaaagcaa aaaattaaaa ggtaaagttc ttactatagg
      601 aataacaaat caaagagaga caactattct atgggataaa ataactggca aaccagttta
      661 caatgcaatt gtttggcaag atagaagagc atcaaattat tgtaaaaaac ttattaaaca
      721 aaaaaaagaa actataattt ataataagac cggtcttctt atcgacgctt atttttcagc
      781 aacaaaaata aaatggatca tagacaatgt tgctaaagca agagaattaa taaaaaaaaa
      841 aagattgtta tttggaacag tagatagttt ccttttgtgg aaattaacaa atggagttga
      901 acatgctaca gacgctacaa acgcaagtag gacgatgcta tttaatattt ctacaaatca
      961 gtgggatcat caaatcttaa agatgttaaa tattccaaaa aatattttac ctaccgtaaa
     1021 agattgttca gctgattttg gtcatacaga tcctttatta actggaaaat cttatccaat
     1081 tactggaatt gtaggagacc aacaatcggc aactataggt caatgctgtt ttgaacctgg
     1141 gtcattaaaa agcacctatg gaactggcgc gtttgtttta ttaaatactg gatcaaaaaa
     1201 aatttattca aaaaatagat tattaactac gataggttat agaataaatg gaaaaactac
     1261 ttatgcttta gaaggttcaa tttttatagc aggagcaggt gttcaatggt tgagagataa
     1321 aattaaattt attaaaaaag cttcagacac agaaaaaatc attagatcat taaaatcaaa
     1381 caaaggcata tatttagtac ctgcttttac tggacttggt gcaccttact gggctgttaa
     1441 tgcacgaggt gttttatctg gactaacaag agatacagga ccaagtgaaa ttgtaagagc
     1501 aactgtagag tctgttgcat atcaaacttt tgatttattt gaagcaatga agaatgatgg
     1561 attaagacca aaaataatta aagttgatgg aggaatggta aaaaatagtt ggttttcaca
     1621 atttttatca gacattttaa
//
LOCUS       DTVV01000189            1170 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1487507, whole genome shotgun sequence.
ACCESSION   DTVV01000189 DTVV01000000
VERSION     DTVV01000189.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1487507"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="EYO86_02250"
     CDS             <1..935
                     /locus_tag="EYO86_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HIC41888.1"
                     /translation="YADPTDPKDFEKLIDDRTRAIYAETLPNPYLRVFPIKEVADIGR
                     KHNIPLIMDNTASPIICKPIEHGAAVVVHSLTKYIGGHGTVIGGCLIDGGNFDWTTDK
                     KRQPLFNEPDASYGGAVWGEVVPQLTGANVAYAVRARVVLLRDLGAALAPDNAFGIIQ
                     GLETVPLRMKQHCSNAQKIVDYLTKHKNVEKVIYPNLHTGEIGDRAKRYFNGGNGGLV
                     GIEVNGGVEAGKKFIEALKMFYHVANIGDARSLAIHPATTTHSQLNEEELLAAGVTPG
                     YVRLSIGIEHPEDIISDLDQALEASGKANLKAVS"
     gene            complement(941..>1170)
                     /locus_tag="EYO86_02255"
     CDS             complement(941..>1170)
                     /locus_tag="EYO86_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HIC41889.1"
                     /translation="IAYLLFFLIIFLNIYYIKLNLDLKSLLLFDIAILIQIFLGIITL
                     LSGMEIKYASLHQIGSILVLSSYLYIFYKNT"
BASE COUNT          444 a          157 c          218 g          351 t
ORIGIN      
        1 gatacgccga tcctactgat ccaaaagact ttgaaaaact tattgatgat agaactagag
       61 ctatttatgc tgaaacttta ccaaatccgt atttaagagt ttttcctatc aaagaagtag
      121 ctgatattgg aaggaaacat aatataccgt taataatgga taacactgct tcaccaataa
      181 tttgtaaacc gattgaacat ggcgctgctg tagtagttca ttcacttact aaatatattg
      241 gtggtcatgg tactgtaatc ggaggatgtt tgatagatgg aggaaatttt gattggacaa
      301 ctgataaaaa aagacaacct ttatttaatg agcctgatgc aagttacgga ggcgctgtat
      361 ggggtgaagt agtacctcag ttaacaggag caaatgttgc ttatgctgta cgtgcaagag
      421 tagttttatt aagagatctt ggggcggctt tagctcctga taatgctttt ggtatcattc
      481 aaggattgga aacagtacca ctaagaatga aacaacattg ttcaaatgca caaaaaattg
      541 ttgattattt aactaaacat aaaaatgtag aaaaagttat atatcctaat ctacacacag
      601 gtgaaattgg agacagagcc aaaagatact tcaatggtgg taatggtgga ttagttggga
      661 ttgaagttaa cggtggtgtt gaagctggta aaaaatttat agaagcttta aaaatgtttt
      721 atcatgttgc gaatattggc gatgcaagaa gtctagcaat acaccctgct actacgaccc
      781 acagccaatt aaatgaagaa gaacttttag cagcaggtgt aactcctggt tatgttagac
      841 tttcaatagg aattgaacat ccagaggata ttatatctga tttagaccaa gctttagaag
      901 catcaggaaa agctaatttg aaagcagtga gttaaagtta ttaagtgttt ttataaaaaa
      961 tatataaata gctacttaaa actaagattg aaccaatttg atgtagagaa gcatacttaa
     1021 tctccatacc acttaataaa gtaataattc ctaaaaaaat ttgaattaat atagcaatat
     1081 caaaaagtaa aagacttttt aaatccaaat ttaatttaat gtaataaatg tttaaaaata
     1141 taattaaaaa aaataaaaga taagcaataa
//
LOCUS       DTVV01000190            4493 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1488724, whole genome shotgun sequence.
ACCESSION   DTVV01000190 DTVV01000000
VERSION     DTVV01000190.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1488724"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1179..2120
                     /locus_tag="EYO86_02260"
     CDS             1179..2120
                     /locus_tag="EYO86_02260"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIC41890.1"
                     /translation="MIKYFDFEKAVEQIDNKIKELQDSDDAENSKTIDKLNIEKKNII
                     TKIYSNLNSWQKVQIARHPDRPHTTDYINNIFSNFIPLSGDKKFGEDPAIVCGMGKII
                     DQSVLIIGNEKGSSMETRIRHNFGMAKPEGYRKAQRIMYLAEKLNLPIVTFVDTAGAF
                     PGKEAEERGQSESIASSIATCLKVKTPIITVIIGEGGSGGAIAIATADKILMLENSIY
                     SVISPEGCSSILWRSPDFVRQAAEALKLTADDCLGLGIVDEIIPEVAGGAHRFANQQI
                     EIVKKIILKKLNALKGISLDNLVLKRNEKFLNITSNN"
     gene            complement(2117..>4493)
                     /gene="secA"
                     /locus_tag="EYO86_02265"
     CDS             complement(2117..>4493)
                     /gene="secA"
                     /locus_tag="EYO86_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIC41891.1"
                     /translation="KNLIKEGSALDDLLIEVFAVVREASKRTLSLRHFDVQLIGGIIL
                     HQGMIAEMKTGEGKTLVATLAAYLNSLSGDPVHIVTVNDYLADRDSHWMGEIYKFLGL
                     TVGCVTSKTEEVDKVKQYNCDIIYATNNEIGFDYLRDNLKNSYESLCFKKEGFAIVDE
                     VDSILIDEARTPLVISGQSDNSVDIYPKINSIVKFLKDEDFEINEESKSVLLSSNGME
                     VTERLLLKNHLIKGGTLQDLDNMGLNHNIIQGLRAHHLFIRDKDYIIKDKSVVIIDDL
                     SGRPMEGRRYGDGLHQAIEAKEGLVIQRENQTIASVTYQNFFRTYKKLSGMTGTANTE
                     AAEFEGIYNLQVVEIPPNLPVNRNDREDQIYMTKKEKYDAVLQLVLERHQIKQPILIG
                     TTSVDNSEILSKLLLKNNIKHNVLNAKVHDQEAETILQAGMPGNVTISTNMAGRGTDI
                     KLGGDDDLSAKNKEISIKAGGLLVIGTERHESRRIDNQLRGRSGRQGDIGESVFFLSL
                     EDDLMRIFGSKTLENVLSKLGVKEGEVITHSLITKSLERAQQKVESHNYDIRKQILKF
                     DDILNDQRKIIYKNRKEILATNDQSDIVQDIIIDFVSDIILECIPPKKYSHEWNAPLL
                     QKKVKDTFFINLPIENWFNEDEVDEEEIKKRILDQVNQKYEEKRNKYSKELLSFAEKR
                     VMLFQIDKNWREHLAAMDTLRSSVNLRAMGGKDPFYEYKKESFDYFNQMLSEQDEKVL
                     KTLFNLELIASNEDRTVVKKIDPKNIVTKKIGRNEPCPCGSGKKYKTCHGN"
BASE COUNT         1500 a          714 c          671 g         1608 t
ORIGIN      
        1 aaaattgata ttttttgtct tattcttgaa gaaagcttta atgaagctaa cttgttaaac
       61 gcactattac aagaaactgg aaatataaat gatgagtact ttcaatatct ttatgataaa
      121 cttaatcaac ctaatttaga ggattcagat cccaatagtt ttgaaattaa tgaaaaggaa
      181 atttttttat atagtgcaat gcatagagta gggaatatag ctttaagtca aaagcttctt
      241 caattagatc caattaattt atcaatgcca attattttaa gtagctcaac tgatattgaa
      301 ttgcgcttaa aagctgctca tgctgcatac aacaatggct ttcttaaaat tgatagtttg
      361 gcagctttat accaaatcgt tgattttagc tctgaagaat taaacgatcc atcaagtgtt
      421 gtatcaataa ctaatggtaa tattgagatg gggatggctt attattatca attgagtaat
      481 attcaactgt tgccagttaa tagattagaa gcaattttga gtttctggaa atttgcagaa
      541 aaaaataatt tagagttaat agcatattca ttatcacaaa agggtcttga tacaattgaa
      601 ccttctaatg aattatctaa atacggagct gaagttgtta aagcgtatgt gcgtagtggc
      661 aatatcgata aggctgaaaa atggttatta ttttcggaaa attattctga agatttaaca
      721 aatttacaca aactacaatc agcagtatta ttattaaatt tatttaaagt tgatgaatca
      781 tcacaattta ttgatatttt gttaaaaaat ttacaaaaca tggaaaagat ttctatcaat
      841 aataatgatc aaaccaatgc tcttaaaaat gaaatactat ttgtggtatt ttctcttttg
      901 aataatagag atcaaaatcc ttttaatttg aatagaaaag ttgatgaacc aaggttcata
      961 ccttctgcat atataatgaa taatataaga gattgtatta aaaataataa tcatgcggaa
     1021 ttattattat caattcttgt atctcttgac ggtaaaaatt gggctgaact tcatcctgaa
     1081 catttacgtt tgattataat tgcacttgaa gagtataaag atggctttct tttaaatgat
     1141 attatcatgg aaattttaga acaaaataaa attatttaat gattaaatat tttgatttcg
     1201 aaaaagctgt tgaacagata gacaacaaga tcaaagagtt acaagatagt gatgatgccg
     1261 agaatagtaa gacaattgat aaattaaata tagaaaaaaa aaatattatt acaaaaattt
     1321 attccaattt aaattcatgg caaaaagttc aaattgccag acatcctgat agaccacata
     1381 ccacagatta tattaacaat attttctcta attttatccc tttgtctgga gacaaaaaat
     1441 ttggtgaaga tcctgctatt gtttgtggaa tgggaaaaat aatagatcaa tctgttttaa
     1501 taataggtaa tgaaaaagga agttcaatgg aaactcgtat cagacataat tttggaatgg
     1561 caaaacctga aggctatcga aaggctcaac gcattatgta tttggcagag aagcttaatc
     1621 ttcccatagt tacttttgtt gatacggctg gagcttttcc aggcaaagaa gctgaagaac
     1681 gaggacagtc agagtctatt gcttcttcaa ttgctacatg tttaaaagtg aaaacaccta
     1741 taataactgt aattattggt gaaggagggt cagggggagc tattgcaata gcaactgcag
     1801 ataaaattct tatgttggaa aattcaatat attcagtaat ttcaccggaa ggatgttctt
     1861 ctatattatg gcgcagtcct gattttgttc gacaagcagc agaagcatta aaattaacag
     1921 ctgatgactg cttaggttta ggtattgttg atgagataat tccagaagta gctggagggg
     1981 cacatagatt tgctaatcaa caaattgaaa tcgttaagaa aattatttta aaaaaattaa
     2041 acgcattaaa aggtatttct cttgataatt tggttttaaa acgtaatgaa aaatttttga
     2101 atattacttc aaataattaa ttaccatgac aagttttgta tttcttacct gagccacaag
     2161 ggcatggctc attgcgacct attttttttg taacaatatt tttaggatca atttttttta
     2221 caacagttct atcttcattg gatgctatta attccaagtt gaaaagtgtt ttaagaactt
     2281 tttcatcctg ctcagatagc atttgattaa aataatcaaa agattctttt ttatactcat
     2341 aaaaaggatc cttacctccc atagccctca aattaacact acttcttaaa gtatccattg
     2401 ccgctaagtg ttccctccaa ttcttatcta tttgaaacaa cattactctt ttttctgcaa
     2461 aagagagcaa ttctttagaa tatttatttc ttttttcttc atatttttga tttacttgat
     2521 ctaaaattct ttttttaatt tcttcttcat ctacttcatc ttcattaaac cagttttcaa
     2581 ttggtaaatt aataaaaaaa gtatctttga ctttcttttg tagaagagga gcattccact
     2641 catggctata tttttttggg ggtatgcatt ccagaatgat atcactaaca aaatcaataa
     2701 ttatatcttg aacaatatct gattgatcat tggtggctaa aatttctttt ctgtttttgt
     2761 agataatttt tctttgatca ttaagaatat catcaaattt caaaatttgt tttctgatat
     2821 cataattatg agattcaact ttttgttgtg ccctctctaa agatttggtt attagagaat
     2881 gagtaatgac ttcaccctct ttaacgccta gcttagacaa aacattttca agagttttag
     2941 atccaaaaat cctcatcaaa tcatcttcta atgatagaaa aaatacactt tcaccaatat
     3001 ctccctgcct tcctgatcgt cctcgcaatt ggttatcaat gcgacggctt tcatgtcttt
     3061 cagtgcctat aaccaataga cctccagctt taattgatat ttctttattt tttgcactca
     3121 gatcatcatc gccgcctaac ttaatatctg ttccccttcc agccatgttg gtagagatag
     3181 tgacattacc aggcattcct gcttgtagga ttgtttccgc ttcctgatca tggacttttg
     3241 cattaagcac attgtgtttg atattatttt ttaatagcag tttggataaa atttcagaat
     3301 tatctacaga ggtagtgccg attaatatgg gttgtttaat ttggtgacgt tcaagaacta
     3361 attgcaggac agcatcatat ttttcttttt ttgtcatata aatttgatct tctcgatcat
     3421 tgcgattgac aggaagattg ggagggattt ctacaacttg caaattataa ataccttcaa
     3481 attctgcagc ttccgtgttt gccgtacccg tcattccact taatttttta tatgtgcgaa
     3541 aaaaattttg ataagttact gaggctatcg tttgattttc tctttgaatc accaaacctt
     3601 cttttgcctc aatggcctga tgtaaaccat caccatatct gcggccttcc attggccttc
     3661 ctgataaatc atcgataata acaacagatt tatctttaat aatataatct ttatccctta
     3721 taaataaatg gtgagctctt aatccctgaa taatattatg gttcagtccc atattatcaa
     3781 gatcttgtaa tgtgccgcct tttatcaaat gatttttcag caataatctt tcagtaacct
     3841 ccattccgtt actagataga agaactgatt tactttcttc attaatttca aaatcttcat
     3901 ctttaagaaa tttaacgatg gagttgattt tgggatatat atcaacggaa ttgtcagatt
     3961 ggccagagat tactaaagga gtgcgggctt catctattaa aatactatca acctcatcaa
     4021 caattgcaaa tccttctttt ttaaagcata gagattcata gctatttttt agattatcac
     4081 gcaaataatc aaatccaatt tcattatttg ttgcgtaaat aatatcgcaa ttatattgtt
     4141 ttactttatc tacctcttct gtttttgatg ttacacaacc tactgttaaa cctaaaaatt
     4201 tatatatttc acccatccaa tgggagtctc tgtctgcaag ataatcatta accgtaacaa
     4261 tatgcactgg atctccagat aatgaattta gataggcagc aagtgtagcc acaagtgttt
     4321 tgccctcacc tgttttcatt tcagcaatca ttccttgatg caaaattatt ccaccaatta
     4381 attgcacatc aaaatgtctt aagctaagtg ttctcttgga agcctctcta acgaccgcaa
     4441 atacttcaat taaaagatca tcaagagccg atccttcttt aataagattt tta
//
LOCUS       DTVV01000191            1101 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1490897, whole genome shotgun sequence.
ACCESSION   DTVV01000191 DTVV01000000
VERSION     DTVV01000191.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1490897"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(28..429)
                     /locus_tag="EYO86_02270"
     CDS             complement(28..429)
                     /locus_tag="EYO86_02270"
                     /inference="COORDINATES: protein motif:HMM:PF06172.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIC41892.1"
                     /translation="MNKSDEIIKILHMIPHPEGGHFKESYRDKNISLIYYLLKKNEKS
                     HWHRLKKNEILHFYDGAPLIVCLSTDGNTFQEVIINNNKDQVSHIVVRSGTWFSMESK
                     GEYSLIGCTVAPPFNYDDFELAPKNWKPGKS"
     gene            complement(441..1034)
                     /gene="ribA"
                     /locus_tag="EYO86_02275"
     CDS             complement(441..1034)
                     /gene="ribA"
                     /locus_tag="EYO86_02275"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIC41893.1"
                     /translation="MKKNLIKFIETSELPTKFGHFKVHAFTEIRSNKDHLAITIGNLN
                     ETDSILSRIHSQCITGESFFSLRCDCRYQLTESLAQIAKKGCGIIFYLQQEGRGIGLS
                     NKIRAYKLQDDGLDTVEANHHLGFNEDERDYEIVSSMADYLEINTIDLMTNNPKKIDA
                     IKKFGLKVNKRISIKSESNDYNKSYLNTKAKKLGHLM"
BASE COUNT          367 a          171 c          141 g          422 t
ORIGIN      
        1 aaaaaagtta acaaaaggag atttagacta agattttcca ggtttccagt tttttggagc
       61 tagttcaaaa tcatcgtaat taaaaggtgg tgcaactgtg caaccaatta aagaatattc
      121 accttttgat tccatactaa accaagttcc acttctaaca acaatatgag atacttgatc
      181 cttgttatta tttattatta cttcttgaaa agtgtttcca tctgttgata aacaaactat
      241 tagaggggcg ccatcgtaaa aatgcagaat ttcatttttt tttaaacgat gccagtgaga
      301 tttttcattt ttttttaaaa gataataaat taaactaata tttttatcgc ggtaagactc
      361 tttgaaatga ccaccctcag gatgcggtat catgtgaagt atttttatta tttcatcact
      421 tttgttcatt tcaaaaaaaa ttacattaaa tgaccaagtt tttttgcttt tgtattcaaa
      481 taacttttat tataatcatt agattcactt ttgattgaaa ttcttttatt tactttgagt
      541 ccaaattttt taatagcatc aattttttta gggttatttg tcattaagtc gattgtatta
      601 atttctaaat aatcagccat tgatgaaact atttcataat ctctttcgtc ttcattaaaa
      661 ccaagatgat gatttgcttc tactgtatct aagccatcat cctgtaattt ataagcacgt
      721 attttatttg ataaaccaat tcctctacct tcctgctgta aataaaaaat tatgccacac
      781 cccttttttg caatttgagc taaagattcg gtgagctgat aacgacaatc acatcgtaaa
      841 ctaaaaaatg attcaccagt aatgcactga gaatgaattc ttgataatat tgaatccgtt
      901 tcattaagat ttcctatggt aatagctaaa tgatctttat tactacgaat ttcagtaaaa
      961 gcatgaactt taaaatgacc aaatttagtg ggcaattcac ttgtttcaat gaatttaata
     1021 agattttttt tcataatata attgcctatt ataactatta caccaagtac ccaagttgtt
     1081 tcataggtat tttaagttca t
//
LOCUS       DTVV01000192            1098 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_149699, whole genome shotgun sequence.
ACCESSION   DTVV01000192 DTVV01000000
VERSION     DTVV01000192.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_149699"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="EYO86_02280"
     CDS             <1..155
                     /locus_tag="EYO86_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130867.1"
                     /note="CysK; forms a complex with serine acetyltransferase
                     CysE; functions in cysteine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIC41894.1"
                     /translation="NIAGAIKLAKELGPGKTIVTVLCDKSDRYHSKLFNKEFLIINNL
                     PIPNWL"
     gene            complement(142..519)
                     /locus_tag="EYO86_02285"
     CDS             complement(142..519)
                     /locus_tag="EYO86_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIC41895.1"
                     /translation="MSNFEDVKDFMTTYGQEVKTKSEFPEEQIIKLRIDLIKEELNEL
                     TDAIKQRNLVEVADALTDILYVTYGAGHSFGINLDLCFAEVQRSNMSKLGEDGKPIYN
                     EHGKVMKGSNYTKPNLKQFIKAS"
     gene            complement(571..>1098)
                     /locus_tag="EYO86_02290"
     CDS             complement(571..>1098)
                     /locus_tag="EYO86_02290"
                     /inference="COORDINATES: protein motif:HMM:PF02163.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41896.1"
                     /translation="SEKFISIDLLRDNTIITKKFELKFNSELQKYLIGISSTNTPSIK
                     RYLFFESFSQSLYFIPNYYLSTYKYLKQSINNNTITKELSGPIGIVKMADQLMLDKIK
                     GIVLLFIVISLFVGIFNLIPVPLLDGGHIVYFIIRRIFSDSLPEFVTKIYLIVGFTII
                     SFLFLVVTFNDIFYK"
BASE COUNT          397 a          160 c          155 g          386 t
ORIGIN      
        1 tcaatattgc tggagcgata aaattagcta aagaattagg acctggaaaa acaatcgtca
       61 cagtactttg tgacaaatca gataggtacc atagcaaatt gttcaacaag gaatttttaa
      121 taataaataa cttgccaatt cctaactggc tttaatgaat tgttttaagt ttggttttgt
      181 ataattggaa cctttcataa ccttgccatg ttcattgtat attggtttgc catcttcacc
      241 taacttactc atattggaac gttggacttc ggcaaaacac agatccaaat ttatgccaaa
      301 agaatgccct gctccataag tgacatatag aatatctgtt aatgcatcgg caacttcaac
      361 taaatttctt tgtttaatag cgtcggtaag ttcatttaac tcttctttga taagatcaat
      421 gcgcagttta ataatttgtt cttcgggaaa ctcagattta gttttaacct cttggccgta
      481 ggtagtcata aaatctttta catcttcaaa attactcata ttttaccctc aataattatc
      541 acaaagttta gttatattaa ttctgttaat ttacttataa aaaatatcat taaaagtgac
      601 tacaagaaat aagaaagaaa taattgtaaa accaactata agatatattt tcgtaacaaa
      661 ttcaggtaat gaatcagaaa aaatacgtct tataatgaag tatacaatgt gacctccatc
      721 aagtaacggc acaggtatta aattaaatat tcctacaaaa agtgaaatta ctataaataa
      781 tagtacaata cccttaattt tatctagcat taattgatcg gccatcttaa ctatacctat
      841 tggaccagat aattcctttg taattgtgtt gttatttatt gattgtttta agtacttata
      901 ggtggataaa taataatttg ggatgaaata taaagattga gaaaatgatt caaaaaataa
      961 ataacgtttt atactagggg tattagtact tgaaatacca attaaatatt tttgcaattc
     1021 cgagttgaac tttaattcaa atttttttgt tataatagta ttgtcgcgca acagatcaat
     1081 tgaaatgaat ttttctga
//
LOCUS       DTVV01000193            1015 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1498405, whole genome shotgun sequence.
ACCESSION   DTVV01000193 DTVV01000000
VERSION     DTVV01000193.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1498405"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            196..906
                     /locus_tag="EYO86_02295"
     CDS             196..906
                     /locus_tag="EYO86_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIC41897.1"
                     /translation="MNILIIAAHPDDEVLGMGGTIAKHTSQHDTVSIIYMATGITGRR
                     ELSESEYEIKNIPKKIQEDWQQEIGKLRQDANKSARLLKVKNVKFFDFPDNEMDGIHL
                     LKVVKVIEKEIKSAKPDRIYTNHYGDLNVDHKVVYNATLVACRPTNFPVKEILSFEVL
                     SSTEWGYPYNFNPNYFINIEKYLGKKIKAMELFVNEIRKFPHPRSSENIKHVARRWGS
                     VSGFNAAEAFELIRRLDK"
     gene            complement(918..>1015)
                     /locus_tag="EYO86_02300"
     CDS             complement(918..>1015)
                     /locus_tag="EYO86_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIC41898.1"
                     /translation="KLKNSKGPDASTSIDTEVLHEMISFIRTIEK"
BASE COUNT          389 a          143 c          153 g          330 t
ORIGIN      
        1 tagaccaccc gttactaaaa tcttcataac taaaacctta gttgcagatt aataaaatct
       61 tgtactgatt gttcataaat tttttctaac aaattatctt gaatgcatta aaacaaatat
      121 tttcagattc agaattatta gacaaaaaga caattttgca taatcattaa taaactaagg
      181 tattcttttt tttccatgaa cattttaata attgccgcgc atcctgatga tgaagtttta
      241 ggaatggggg gtacaattgc aaaacatact tctcaacatg atactgtgtc aataatttac
      301 atggctacag gtattactgg gagaagagaa ctttcggaat cagaatatga aataaaaaac
      361 attcctaaaa aaattcaaga agattggcaa caagaaattg gaaaattacg acaggatgca
      421 aacaagtctg caagacttct taaagtaaaa aatgtcaaat tttttgattt tccagataat
      481 gaaatggatg gaatacattt actaaaagtc gttaaagtta tagaaaaaga aattaaatct
      541 gccaaaccag ataggatata cacaaatcat tatggcgatt taaatgtgga tcataaagta
      601 gtctacaatg ctacacttgt agcttgtaga cctactaatt ttcctgtgaa agaaatacta
      661 tcttttgaag ttttatcatc tacagagtgg ggctatccat ataattttaa tccaaattat
      721 tttatcaata ttgaaaaata tcttgggaaa aaaattaagg ctatggaatt attcgtaaac
      781 gaaataagaa aatttcctca cccacgttca tctgaaaata ttaaacatgt tgcaagaagg
      841 tggggttctg tatccggttt taatgccgct gaagcgtttg aactaattag acgacttgat
      901 aaataaaaaa tataattcta tttttcaata gttctaataa aagaaatcat ttcatgaaga
      961 acttctgtat caatagatgt acttgcatct ggacctttgg aattttttaa tttaa
//
LOCUS       DTVV01000194            1479 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1501510, whole genome shotgun sequence.
ACCESSION   DTVV01000194 DTVV01000000
VERSION     DTVV01000194.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1501510"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="EYO86_02305"
     CDS             complement(<1..338)
                     /locus_tag="EYO86_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIC41899.1"
                     /translation="MNTNYKNIAKGGKAVYGGTVGVCMLDTQFPRIHGDIANARTWSV
                     PVHYRVVPGATPKAAVFDGGKEILDGFIDAAKQLVKMGADGITTNCGFLSLFQEKMAE
                     VVNVPVATSS"
     gene            424..>1479
                     /locus_tag="EYO86_02310"
     CDS             424..>1479
                     /locus_tag="EYO86_02310"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00205.20,HMM:PF02776.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIC41900.1"
                     /translation="MIKKYNTNQLTAAEGMVKLLDAYNVKHIFGLCGDTSLPFYDALY
                     RLDHSINHILTRDERHASYMADAYARVTGKVGICEGPSGGGATYIVPGVVEANESSIA
                     LISLTSDVPISSIGHFPLTELDQEKMFKPLTKWNKKIDKAKKLGKIVRTAFKKSTSGR
                     PGAVHLCFPFDIQTSEIKEDDIWVEKKFSTFPANPLKPNTEKIDKIIKEISKSKNPMI
                     ICGGAIKNCNAEKDLKKFIESQNMVLATSVTGKGTLADEHPNYLGVVGSNGGVLATRE
                     AIKKADLIIFIGCRAGSVTTEKWKYPSSKKKIIHIDIDQKVFSANYKTLISLKSDAKL
                     ALVALNKKIKKTILKVKN"
BASE COUNT          546 a          232 c          248 g          453 t
ORIGIN      
        1 aaagaggaag ttgcaacagg aacatttact acttctgcca ttttttcttg aaatagtgat
       61 aagaaaccac agtttgtggt gataccatct gctcccattt ttaccaattg tttagctgca
      121 tcaataaaac catctaaaat ttctttacca ccatcgaaaa cagctgcttt gggtgttgct
      181 ccaggcacaa ctctataatg aactggtaca ctccaagttc tagcattggc tatgtctccg
      241 tgaattcttg gaaattgtgt atcgagcata caaactccaa ctgtgccgcc gtaaactgct
      301 ttacctcctt tggctatatt tttatagttt gtgttcatat taattgttaa attttacttt
      361 taaatttttc ctaatacaag aatttaaaac tatactagac taaacgagaa aaaaattttt
      421 ttaatgatca aaaaatataa taccaaccag ttaacagctg cagaaggcat ggttaaatta
      481 ttagatgcat acaatgttaa acatatcttt ggactttgtg gagatacttc gttaccattt
      541 tatgatgctt tatacaggct agatcattct ataaatcata ttttaacaag agacgaaaga
      601 cacgccagtt atatggcaga tgcttacgct agagttacag gaaaagttgg aatatgtgaa
      661 ggcccaagcg gaggtggagc cacatacatt gttccgggcg tagttgaagc aaacgaatct
      721 tcaatagctt taatatcttt aacatctgat gttccaatat cctcaattgg tcactttcct
      781 ttgactgaac tggaccaaga aaaaatgttt aagccattaa ctaaatggaa taaaaaaata
      841 gataaagcaa aaaaacttgg caaaatagtt agaacagctt tcaaaaaaag tacatcaggt
      901 agacctggag cggttcattt atgttttcca tttgatattc aaacatcaga aataaaagaa
      961 gatgatattt gggtagaaaa aaaattttct acatttcccg caaatccatt gaaacctaat
     1021 acagaaaaaa ttgacaaaat aattaaagaa atttcaaaat ctaaaaatcc tatgattata
     1081 tgtggtggtg caattaaaaa ttgtaatgct gaaaaggatc tcaaaaaatt tattgaaagc
     1141 caaaatatgg ttttagcaac aagtgttacg ggcaaaggaa ctttagctga cgaacatcca
     1201 aactacttag gagttgtggg aagcaatggt ggtgttttgg ctactagaga agcaattaaa
     1261 aaggctgatt taattatatt tattggttgt cgcgcaggat cagttacaac agaaaaatgg
     1321 aaatatccaa gttcaaagaa aaaaattata catattgata ttgaccaaaa ggttttttct
     1381 gcgaattaca aaacgcttat ttcattaaag tctgatgcaa aattagcttt agttgctcta
     1441 aataaaaaaa ttaaaaaaac gattttaaag gtaaagaat
//
LOCUS       DTVV01000195            1685 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1511326, whole genome shotgun sequence.
ACCESSION   DTVV01000195 DTVV01000000
VERSION     DTVV01000195.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1511326"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            406..648
                     /locus_tag="EYO86_02315"
     CDS             406..648
                     /locus_tag="EYO86_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41901.1"
                     /translation="MQSGTKKFDKWIIEFITEDTGVNPLMGWESSTDTYTELKLEFSS
                     KELAIDYAKKNKIEFELIEPHERKIVKKTYSNNFTK"
     gene            649..1647
                     /locus_tag="EYO86_02320"
     CDS             649..1647
                     /locus_tag="EYO86_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016958689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HIC41902.1"
                     /translation="MFKFFTSKHWFKWSIFGSIFILISTWYQVQLDVKINEWFGEFYD
                     TLQKALTTPGSVTETEFISYLFTFAKIAGLWIIIAVITGYFTSHWVFRWRTAMADYYH
                     EQWLKARLTEGASQRVQEDTLKFARIMEGLGTGLLDSVMTLIAFTPILWGLSKQIDAL
                     PWIGQVDHALVWVAIITALGGTILLALVGIKLPGIEYDIQKEEAAYRKELVMGEDDPI
                     RAAPPTIGQLYNRVRRIHFRSYFNYLYFNCVKWSYFQGMVIVPYLALAPTIVTGAITL
                     GFVQQITRAFGRVEGSLQYLVKSWSIIVEFISVWKRLSEFEKKLDSGKVEHYTETV"
BASE COUNT          545 a          277 c          327 g          536 t
ORIGIN      
        1 aaaccaacct acaaaatggt tctaggcttg cctgggcaaa gctatgctat ggcagttgcc
       61 acaagattag ggttgaaagc cgaaatagtc cgttgcacaa gttgtattaa cagctattca
      121 cggcgaggtt cccccccgtt tccgccaagc ctcccttccc cggtcaattc aacgagcttt
      181 ccatgcaacg cctcatccct gttatcatca tcgtatcgac cgccgtcggt ctagcctctg
      241 atcccgctgg tttctacgat ttcgttggcg agcagcatga aggcctcgac caggcggtgg
      301 ctctccagca aaagtgacaa gccatcggtc aattgaattt cgcccttggc tcccggcagg
      361 gtgcgttcca aacaagcaaa aatttataaa ccaactaaaa ctgctatgca gtctggaaca
      421 aaaaaatttg ataaatggat tattgaattt attactgagg acactggtgt aaatccgtta
      481 atggggtggg aaagttctac cgatacatat actgaattaa aattagagtt ttcatcaaaa
      541 gaattggcca ttgattatgc aaaaaaaaat aagattgaat ttgagttaat tgaacctcat
      601 gaaagaaaaa ttgtaaaaaa aacatactca aataatttca ctaaataaat gtttaaattt
      661 tttacttcta aacattggtt taaatggagc atttttggat caatatttat tttaatatct
      721 acttggtatc aagttcaact agatgttaaa attaatgaat ggtttggtga attctatgat
      781 actttacaaa aagctcttac aacacctggt tctgtaactg aaactgaatt tatttcttat
      841 ttatttactt ttgcaaaaat tgccggtctt tggattataa tcgctgtgat tactggatat
      901 tttacaagtc attgggtttt cagatggaga actgcaatgg ctgattatta tcatgaacaa
      961 tggttaaaag ctcgtttaac agaaggtgct tcacaaagag ttcaggaaga tacattaaaa
     1021 tttgcaagaa ttatggaagg acttggtacg ggattattgg atagtgtaat gacacttatt
     1081 gcttttactc ctatactttg ggggttatca aagcaaattg acgctctccc ctggattggt
     1141 caagttgatc atgctctagt ttgggttgca ataattactg ctttgggagg tacaatttta
     1201 ttagccttag taggaattaa acttccagga atagaatatg atattcaaaa agaagaagct
     1261 gcttatcgta aagaattagt aatgggtgaa gacgatccga tacgtgcggc tccaccaaca
     1321 attggtcaat tatataacag agttagaaga atacatttta gatcatattt caattattta
     1381 tatttcaatt gtgttaaatg gagctacttt cagggaatgg ttattgtgcc atatttagct
     1441 ttagcaccaa ctatagttac tggtgccatt acattaggtt ttgtacaaca aatcacaaga
     1501 gcttttggaa gagttgaagg atctttgcaa tatttagtaa aaagttggtc aataatagtt
     1561 gaatttatct ctgtgtggaa gagattaagt gaatttgaaa aaaaacttga ttcaggtaaa
     1621 gttgaacatt atacggaaac tgtttaatgg ccttagacat aagtttaatt aaaatatttg
     1681 aaaat
//
LOCUS       DTVV01000196            2322 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1515345, whole genome shotgun sequence.
ACCESSION   DTVV01000196 DTVV01000000
VERSION     DTVV01000196.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1515345"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /locus_tag="EYO86_02325"
     CDS             <1..1771
                     /locus_tag="EYO86_02325"
                     /inference="COORDINATES: protein motif:HMM:PF01551.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC41903.1"
                     /translation="LNAAFKKAQITQTQDALKTQCDDVAQAITTFIHSPLNGKILILT
                     DNAFEFDYGPTIVLEHAIDKKNRFYTIYGHLSKKCLKLLHVGQQIRKGQVLAEIGNYP
                     INGNWPPHLHFQIIMNMMGEKENFPGVSEDILIPMWSKISPDPNLILGIPNSFFKKHE
                     HIANLITKRRSLVSNNLSISYNKPLQFLEAKGQYLYDDKGRQYLDCVNNISHVGHCHP
                     KIHEALVNQNEKLNTNTRYIYKIMNDYSEKLLSKFPKKLDTVFFVCTGSEANDLAYRI
                     AQTYTHSRDVLVIDNAYHGHTNTLIDLSPYKFKGKGGKGQKDHVHVTKMPDGIRGDWK
                     YKNKFWIKEYIKDVQKIIDQIYSNNKKLSCFFVESILGCGGQIILPPNYLKETFKLVR
                     KKSALCIIDEVQTGFGRVGNNFWGFEEHGVVPDIITLGKPMGNGHPIAAVVTTKEIAN
                     VFNNGMEYFNSFGGNPVSCAVGNAVLEVIENEQLQKRALDVGKYFLNSLNLIKKNHPK
                     LISEVRGRGLFIGIDFVNNSINEPNKKLASLIINKLRNQGILLSTDGPYHNVIKIKPP
                     LPFTKEDSDFVSQELDQLLRSLY"
     gene            complement(1768..>2322)
                     /locus_tag="EYO86_02330"
     CDS             complement(1768..>2322)
                     /locus_tag="EYO86_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41904.1"
                     /translation="LIIAIGPQNLFIIRQGLRKSFVFIVCLICSLSDTILIIIGIAFS
                     SYLSNISPLLIYILKIIGSIWLITYGILKIKNSFKLKEFKIDDKNEIIQNVILTTLTL
                     TFLNPHVYLDTVILIGTIALNFHYKLSFGTGVVISSFVFFFCLGYFSKFISKFIQNNK
                     SWFWIDNIFGTLMICYGLFFILSI"
BASE COUNT          884 a          319 c          370 g          749 t
ORIGIN      
        1 attaaatgca gcatttaaaa aggctcaaat aacacaaact caagacgcct taaaaactca
       61 atgtgatgat gttgctcaag cgataacaac ttttattcat tcaccgttaa atggtaagat
      121 actgatactc acagataatg cttttgaatt tgactatggt ccgacaattg tcctagagca
      181 cgcaatagat aaaaaaaata gattttatac aatctatggc catctgtcta aaaaatgctt
      241 aaaattattg catgttgggc agcaaattag aaaagggcaa gtactagctg aaataggtaa
      301 ttatcctatc aatggtaatt ggccgcctca tttacatttt caaattataa tgaatatgat
      361 gggggaaaaa gaaaattttc caggtgtatc tgaagacatc ctaattccta tgtggtcaaa
      421 aatatcacct gatccaaatt taattttggg tattcccaat tcttttttta aaaaacacga
      481 acatattgct aatttaatta ctaaaagacg ttcactagtt agcaataatt tatccatttc
      541 ttataacaaa ccccttcaat tccttgaagc caaaggtcag tatttatatg atgataaagg
      601 tagacaatat ttggattgtg taaataatat ttcacatgtt ggacattgtc atccaaaaat
      661 ccacgaagcc ttagttaatc agaatgaaaa actaaatacc aatacaagat atatttataa
      721 aataatgaat gattacagtg aaaaactttt aagtaaattt cctaaaaaat tagatacagt
      781 tttttttgtt tgcacaggat cagaggcaaa cgatttggct taccgtattg cacaaacata
      841 cacacattca agagatgttt tagttattga taatgcttat catggacaca ccaataccct
      901 tatagatctc agtccatata aatttaaagg aaagggagga aaaggtcaaa aagatcatgt
      961 acatgtcaca aaaatgcctg atggaattag aggagattgg aagtataaaa ataaattttg
     1021 gataaaggaa tatattaaag atgttcaaaa gataattgat caaatttatt ccaataataa
     1081 aaaattatct tgtttttttg tggagagtat tttgggttgt ggcggtcaaa ttattttacc
     1141 acctaactac cttaaggaaa cttttaaact tgttagaaaa aaaagtgcat tatgtattat
     1201 tgatgaagtt cagacaggtt ttggtcgtgt aggtaataat ttttggggat ttgaagagca
     1261 tggagttgtt ccagatatta taacattagg caagcccatg ggaaatgggc atcccattgc
     1321 agcggtagtt acaactaaag aaattgcgaa tgtgtttaat aatggaatgg aatatttcaa
     1381 ttcttttggt ggcaatccag tttcttgcgc cgtagggaat gccgtacttg aggttattga
     1441 aaatgaacag ttgcaaaaac gtgctctgga tgttggtaaa tattttctta attctttaaa
     1501 tttaattaag aaaaatcatc caaaattaat ttcagaagta agggggaggg ggttatttat
     1561 agggattgat tttgttaata attcaataaa tgaacctaat aaaaaattgg catcacttat
     1621 tatcaacaaa ttaagaaacc aaggtatttt gttgagtaca gatggcccat atcacaatgt
     1681 aattaagatt aagcctcctt tgccttttac taaagaagat agtgattttg tatcgcaaga
     1741 acttgatcaa cttttgaggt ctttatatta gatacttaat ataaaaaata gtccataaca
     1801 aatcattaag gttccaaaaa tattgtctat ccagaaccat gatttattat tttgtataaa
     1861 ttttgatatg aatttagaaa aatatcccaa acaaaagaag aaaacaaaac tagaaataac
     1921 aacacctgtt ccaaaagata atttgtaatg aaaatttaac gcaattgttc caatcaaaat
     1981 aactgtatcc agataaacat gaggatttaa aaaagtaagc gttaatgttg taagtataac
     2041 gttttgaatg atttcgttct tatcatcaat cttaaactct tttaatttga aagaattttt
     2101 tattttgaga ataccatatg taattagcca tattgatcca ataattttta aaatatagat
     2161 taataaaggc gagatattag aaagataaga cgaaaacgct attcctataa taattaaaat
     2221 agtatcagac aagctacata ttaagcacac aataaaaaca aaacttttcc ttaaaccttg
     2281 tctaattatg aatagatttt gcgggcctat tgcaatgatc aa
//
LOCUS       DTVV01000197            1271 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1517553, whole genome shotgun sequence.
ACCESSION   DTVV01000197 DTVV01000000
VERSION     DTVV01000197.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1517553"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="EYO86_02335"
     CDS             <1..611
                     /locus_tag="EYO86_02335"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIC41905.1"
                     /translation="SLPVFDKIRKEVEIPVLTDVHTIEQCEVVSKHVDVLQIPAFLCR
                     QTDLLIAAAKTGKIINVKKGQFLAPWDMENVTKKIEDSGNKNILVTERGASFGYNTLV
                     SDMRSLPIMAKNGYPVVFDATHSVQQPGGMGNRSGGQREFVEYLARAAVAVGVAAIFM
                     ETHPDPDNAPSDGPTMVPLMKMPNLLKKLLEIDKLIKDVKNN"
     gene            595..>1271
                     /gene="eno"
                     /locus_tag="EYO86_02340"
     CDS             595..>1271
                     /gene="eno"
                     /locus_tag="EYO86_02340"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282055.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIC41906.1"
                     /translation="MSKITNIKARQVFDSRGNPTIESEVHLNDGSKASAIVPSGASTG
                     SHEAFELRDIENKKYLGKSVLKAIEKVNGEISKALTGFSAEDQKKIDKILIELDGTKQ
                     KKRLGANAILSVSLASNKVSAVSKNIPLYKNIGNEKILPLPLMNVINGGSHADNSLRI
                     QEFMIRPDKAKTFKEAINICFLVIQKLRSLISSKNFSTNLGDEGGFAPSLSNNEEAIE
                     FILKAIE"
BASE COUNT          487 a          177 c          234 g          373 t
ORIGIN      
        1 aatcacttcc agtttttgat aaaattagaa aggaagttga gataccagtc ttaactgatg
       61 tacatacaat tgaacaatgt gaagttgttt ctaaacatgt agatgttcta caaataccag
      121 cttttttgtg tagacaaaca gatttattga ttgcagcagc taaaacagga aaaataatta
      181 atgttaaaaa gggtcaattc ttagctccat gggatatgga aaatgttaca aaaaaaatag
      241 aggattcagg aaataaaaac attttggtta ccgaacgagg cgctagtttt ggatataaca
      301 ctttagtttc tgatatgaga tctttaccta taatggcaaa aaatggttat ccagttgttt
      361 ttgatgcaac tcattcagta caacaaccag gtggtatggg taacagaagt ggcggtcaaa
      421 gagaatttgt agaatatctt gcccgagctg ctgtagcagt gggtgttgca gcaattttta
      481 tggaaactca tcctgatcca gataatgctc catcggatgg tcctacaatg gttccgctta
      541 tgaaaatgcc aaatttgtta aaaaaattac ttgaaattga taaattaata aaagatgtca
      601 aaaataacta atatcaaagc ccgacaggtt tttgatagta gaggaaatcc tacaatagaa
      661 tccgaagttc atttaaatga tgggagcaaa gcatctgcta ttgttccttc tggtgcctct
      721 acaggatcac acgaagcttt cgaactaaga gatattgaaa ataaaaaata tttgggaaaa
      781 agcgtcttaa aagcaataga aaaagtaaat ggagaaatat ctaaagcact gactggtttt
      841 tctgcagaag atcaaaaaaa aattgacaag attttaattg agctagatgg aactaaacag
      901 aaaaagagat taggagcgaa tgcaattttg tctgtttctc tagcttcaaa taaagtatcg
      961 gcagtttcaa aaaatatacc tctatataaa aatataggta atgaaaaaat attaccctta
     1021 ccactgatga atgttattaa tggagggtct catgctgata actcattgag aattcaagaa
     1081 tttatgatac gacctgataa ggcaaaaact tttaaagaag ctattaatat atgtttttta
     1141 gtaatacaaa aattaagatc tcttataagt agcaaaaact tttcaacaaa tctaggggat
     1201 gaaggaggtt ttgcgccatc actatcaaac aatgaagaag caattgaatt tattttaaaa
     1261 gcaatagaaa a
//
LOCUS       DTVV01000198            7730 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1518560, whole genome shotgun sequence.
ACCESSION   DTVV01000198 DTVV01000000
VERSION     DTVV01000198.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1518560"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="EYO86_02345"
     CDS             <1..562
                     /locus_tag="EYO86_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIC41907.1"
                     /translation="AHLNIDIKKATELQRKYYRQHGTTLRGLMDNHNVDPDHFLSEVH
                     QLDYSIVGPNFKLNRELKKLKGRKIIYTNANRQHANDVLIRLELTNVFDEIFDIKTAN
                     YIPKPEASPYEQIISEFNIDPITTIMFDDIAKNLVPAKNVGFASVWIDVGYENFSDDI
                     AKSKKYLDYETKDLSLFLDEVNKEKI"
     gene            559..1389
                     /gene="dapD"
                     /locus_tag="EYO86_02350"
     CDS             559..1389
                     /gene="dapD"
                     /locus_tag="EYO86_02350"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIC41908.1"
                     /translation="MSDLKNIINDAFENKGVVSLTTTGRIRDAVNSTLDQLDEGSLRV
                     SEKVDGEWIVNQWLKKAILLSFRLNDNEVIKAPHASWFDKIPSKTVNWTREDHERAGF
                     RYVPDAVVRKSAFIAKGVVLMPCFVNLGAYVDEGTMIDTWATVGSCAQIGKKCHISGG
                     AGIGGVLEPLQANPVIIEDNCFVGARSEVAEGVIVGEGSVLSMGVFIGASTKIIDRTT
                     GETHMGKVPPYSVVVPGSLPGKSLPDGSVGPSLYCTVIVKRVDEKTRSKTSINDLLRD
                     "
     gene            1389..2570
                     /gene="dapE"
                     /locus_tag="EYO86_02355"
     CDS             1389..2570
                     /gene="dapE"
                     /locus_tag="EYO86_02355"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:PRK13009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIC41909.1"
                     /translation="MTKINFDPINLTQALVQCPSVTPKDEGALDIVENHLSHLGFKCT
                     RLPFSDKNSYDVDNLFASIGSTGKHLAFAGHTDVVPPGNEDSWKYPPFSAKINGDKLY
                     GRGSEDMKSNIACFISATHAFIEKHGANFNGQLSFIITGDEEKDAINGTSKMLEWTKQ
                     NNIIFDQCIVGEPTSNKSIGDKIKIGRRGSISFFITVKGVQGHTANAHRAENSAHHLT
                     TLLNNIISKPLDKGNDNFLPTSIQIATIDIGNTAQNIIPEFAKATVNIRFNDMHTSES
                     LIEWMQNHIDSVFKNIDKSSCSFTIDSSAEAFLTKPGSLSKLMSDSIAEVTSKNSKTE
                     MATDGGTSDARFIKDYCEVAELGIRNQTLHQVNEFVYLNDLKDLQKIYLKILENYFSK
                     N"
     gene            complement(2567..3496)
                     /locus_tag="EYO86_02360"
     CDS             complement(2567..3496)
                     /locus_tag="EYO86_02360"
                     /inference="COORDINATES: protein motif:HMM:PF02826.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41910.1"
                     /translation="MNILFHSTFINNKQWLRNIKKKFKGKKIYTFKDKPNYDKIDYAI
                     VWNLPDKLFAKLKNLRAIFSLGAGVEHILNLPSYKDTPIVRLKDPALADRIFNHVLSQ
                     ILNHQLKLEHYRKAQKAKIWQNELDTYLNNQIQVGILGAGFIGTIVGQNLQKINYNVV
                     GFKNTLVKKKNFFQIYSKNKLNDFIKSSDIIISILPSTPNTTNFINKNFLRKMKKKSL
                     LINVGRGTSVNEKDLISHLKSNKNFYASLDVFKEEPLSKKHPLWSLKNVIITPHIAGV
                     TVLDSAIEQIHNRWLQITKTGIIKNDVNLKKGY"
     gene            complement(3504..4619)
                     /locus_tag="EYO86_02365"
     CDS             complement(3504..4619)
                     /locus_tag="EYO86_02365"
                     /inference="COORDINATES: protein motif:HMM:PF00534.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HIC41911.1"
                     /translation="MILILTQCFPSRLGGIESLVSNLSVGLSKSERIIVFADRHHMFY
                     DAIYDNNHKDEILVRRISGLKFFRQRRKIKEIKLLIESHKVKLIIADTWKSLELGIDY
                     FNTKNIPSICLAHGNELLSNDLKKAQRIKNTLNKSTAVVANSLYTKKLVQELVKSTII
                     VDYVYPGANDLRNHKEHSLKEVNGEPVILTLARLEKRKSHAHIIHCIKKLLSDFPNIQ
                     YVIAGEGPEKRALQKLVNDKNLQNNVLFVGLVNDEQKKFLFERSSLMVMPTLDESKSR
                     SIEGFGISYLEAAFFGIPSIASNVGGTPEAVINNSTGVIINNIDELYQSIHDLLVDEN
                     KRILFGENAQRRSHENFQWNTVTKKYKSIFDKVVNNS"
     gene            4928..5140
                     /locus_tag="EYO86_02370"
     CDS             4928..5140
                     /locus_tag="EYO86_02370"
                     /inference="COORDINATES: protein motif:HMM:PF12086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 domain-containing protein"
                     /protein_id="HIC41912.1"
                     /translation="MAKKLLSKLEKLVSAIAKRGSSHNTWYGFSNKYHKNQVEKYLAN
                     SYDLYDLDYRQKELDRKGTYNKLYIQ"
     gene            complement(5155..6906)
                     /locus_tag="EYO86_02375"
     CDS             complement(5155..6906)
                     /locus_tag="EYO86_02375"
                     /inference="COORDINATES: protein
                     motif:HMM:PF16188.3,HMM:PF16189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIC41913.1"
                     /translation="MNPLQKIQTWMKDHSIDVFIINRTDEFLGEYIASYAERLQWISN
                     FSGSSGKAIIFQEKAYLFVDGRYSIQAQEQTDSEFFLVKHLQDYWNFLEELCFEGKII
                     AIDPTLHSVEEVEKIQQIVDNRKSSLQYLDNNPIDKYWKTQPFYPQSKAFIHDFKYVG
                     KAGRDKIYEIQNSLASTFIDYYILTSLDSIAWLLNIRGNDIDYTPLLCCFVIIPQKGN
                     VEFFVEEKKIRSIKNELEKFVNFHSFESIYEFIGKIGKDKIVGMDEKQTVYNFKKICL
                     NNDITINYLSNPCTYPKAQKNEIELLGARNANIRDGASITKFLYWLKNIMKISETNEI
                     NAADYLLNLRKENELFYSPSFDTISAFGPHAALPHYRVSPETNLSFENNSIYLVDSGV
                     QYRDGTTDITRTVIFGKATNEQKDRFTRVLKGHIAIATAEFASNSVGSTLDPLARESL
                     QAIGCDYDHGTGHGIGSFLSVHEGPQRIAKNQGLSDGIIYPGMILSNEPGYYKINEYG
                     IRTENLIIACIKSNNTMFFETISWAPIDRDLIEKSILLDNEINWINIYHQDVYNKLSP
                     YLKSQEKEWLTKVTTPL"
     gene            complement(6906..>7730)
                     /locus_tag="EYO86_02380"
     CDS             complement(6906..>7730)
                     /locus_tag="EYO86_02380"
                     /inference="COORDINATES: protein motif:HMM:PF00291.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HIC41914.1"
                     /translation="FKLRGASNKIGQLTKNEKNKGVVAYSSGNHAQAVAYASKLNNIS
                     STIVMPKNAPSIKINNTKRYGSEVVLYDPASGVREDIANDIARTTKKTIIKPYDDLDI
                     IAGQGTAGKEIAEELLDQNIRPDMYLCCCGGGGLIAGTSTYLKHKFPNIEIYSVEPEE
                     FNDTQLSFRSNFITPIKKGAQSICDALLAPQPGDITFAINQQTLTDGLTVSDKEVKST
                     IVVLAENLKIITEPGGAVAASALLNKKLDVENKTIIVMISGGNIDGELFSSIAKEK"
BASE COUNT         2540 a         1255 c         1236 g         2699 t
ORIGIN      
        1 agcacattta aatattgata taaaaaaagc aacagaactt caaagaaaat actataggca
       61 acatggtaca actttgagag gtttgatgga caatcacaat gttgatccag atcactttct
      121 gtctgaagtt catcaattag actattcaat agttggtccc aatttcaaat taaatagaga
      181 attaaaaaaa ttaaaaggca gaaaaattat atatactaat gcaaatagac agcatgcaaa
      241 tgatgtacta attcgcctag aactaacaaa cgtgtttgat gaaatatttg acataaaaac
      301 tgctaactat ataccgaaac ctgaagcatc tccctatgaa caaataatta gtgagttcaa
      361 tattgatccc ataaccacaa ttatgtttga tgacattgct aaaaatcttg ttccggcaaa
      421 aaatgtagga tttgcctctg tgtggattga tgttggatat gagaatttta gtgatgatat
      481 agctaaatca aaaaaatact tagattatga aacaaaagat ttgtccttgt ttttagatga
      541 ggtaaataag gaaaaaatat gagtgattta aaaaatatca ttaatgatgc ttttgaaaat
      601 aagggggtgg tttctttaac tactacaggt agaataaggg atgctgtaaa ctcaacatta
      661 gatcaacttg atgaaggttc attgcgtgta tccgaaaaag ttgatggtga atggattgtt
      721 aatcagtggt taaaaaaagc aattctttta agttttagat taaatgataa tgaagtcatc
      781 aaggcccctc atgcctcttg gtttgataaa attcccagta agactgtgaa ttggacacga
      841 gaagatcatg aacgtgctgg atttaggtat gtgcctgatg ccgttgtaag aaaatcagcc
      901 tttattgcaa aaggcgttgt cctgatgccc tgttttgtaa atctaggtgc atatgttgat
      961 gaaggaacaa tgattgatac gtgggcaact gttggttcat gtgctcaaat tggtaaaaaa
     1021 tgtcatattt ctggtggtgc tggtattggg ggtgttttgg aacctttgca agcaaatcca
     1081 gtaatcatag aggataattg ttttgtaggt gctagaagtg aagtagctga aggggtgata
     1141 gttggagaag gatctgtttt atctatgggt gtatttatag gtgcttcaac taagattatt
     1201 gacagaacga ctggggaaac tcatatgggt aaagtacctc catactctgt tgttgtccct
     1261 ggctctcttc caggaaagtc tcttccagat ggttcagttg ggccttctct ttattgtacg
     1321 gttattgtta aacgtgttga tgaaaaaaca cgttctaaaa cttctatcaa tgatttgctt
     1381 cgtgattaat gactaaaatt aattttgatc ctattaactt aactcaagct ttagttcagt
     1441 gtccaagcgt tactcctaaa gatgaaggtg cgcttgatat agttgaaaat cacttgagtc
     1501 atttaggctt taaatgcact agacttcctt tttctgataa aaattcttat gatgtagata
     1561 atctttttgc ttcaattgga tctacaggaa aacatttggc ctttgcagga catacagatg
     1621 ttgttcctcc aggcaatgaa gactcttgga agtatcctcc tttttcagca aaaattaatg
     1681 gggataaatt atatgggcgt ggctcagagg atatgaaaag taatattgcc tgttttatat
     1741 ctgctactca tgcatttatt gaaaaacacg gcgctaattt caatggacaa ttgtcattca
     1801 taattaccgg agatgaagaa aaagatgcaa taaatggtac gtcaaaaatg ttggaatgga
     1861 ctaagcaaaa caatattatt tttgatcaat gtattgtagg tgagccaaca tctaataaaa
     1921 gtataggtga taaaattaaa attgggagaa gaggatcaat cagtttcttt ataacagtaa
     1981 agggagttca aggacatact gctaatgcac accgcgcaga aaattccgcc catcacttaa
     2041 ctacattgtt aaataatata atttcaaaac cattagacaa aggtaatgat aattttttac
     2101 caacttccat acaaattgcc acaatcgaca taggcaatac tgcacaaaat attattcctg
     2161 aatttgccaa agctacagta aatattaggt ttaatgacat gcatacaagt gaaagcttga
     2221 ttgaatggat gcaaaatcac attgatagtg tttttaaaaa tattgataaa tcaagctgct
     2281 cttttactat agattcaagt gccgaagcat ttctaacaaa gcctggctct cttagcaaat
     2341 taatgtctga ctccatagcc gaagtaacaa gtaaaaatag taaaactgaa atggcaacag
     2401 atggaggaac atccgatgct cgttttatca aagattactg tgaagtagct gagttaggca
     2461 ttagaaatca aactctacat caggttaatg agtttgttta cctaaatgat ttaaaagatc
     2521 ttcaaaaaat atatttaaaa atattggaaa attattttag taaaaattaa taaccttttt
     2581 taagatttac atcattttta attatacctg tttttgtaat ttgtaaccaa cgattatgta
     2641 tttgttctat cgcactatcc aaaacagtaa cacccgcaat atggggtgta atgatcacat
     2701 tttttaaact ccataaagga tgttttttag aaagtggttc ttctttaaaa acatccaaag
     2761 atgcataaaa atttttgttt gatttcaagt gactaattaa atccttttca ttgacagaag
     2821 tacctcttcc aacatttatt aaaagtgatt ttttcttcat ttttcttaaa aaatttttat
     2881 tgataaaatt tgttgtatta ggtgtcgaag gtaaaattga aataataata tcgctacttt
     2941 tgataaaatc atttaattta ttttttgaat atatttggaa aaaatttttt ttctttacaa
     3001 gtgtattttt aaagccaaca acattataat ttattttttg caaattttga ccaacaatag
     3061 tccctataaa accagctcct aaaattccaa cttgtatttg attattcaag tatgtgtcta
     3121 attcattttg ccaaatttta gctttttgtg cctttctgta gtgctcaagt tttaattgat
     3181 ggttcaaaat ctgtgacaaa acatggttaa atatcctatc tgccaaagca ggatctttta
     3241 atctaacgat gggggtatct ttgtaactag gcaaattaag tatatgttca accccggccc
     3301 ctaaagaaaa aatggctctt aaatttttca attttgcaaa caatttgtcg ggtaaattcc
     3361 aaacaattgc ataatcaatt ttgtcatagt taggcttgtc cttaaatgta tagatttttt
     3421 tccctttaaa ttttttttta atatttctta accattgttt gttattgata aaagtagaat
     3481 gaaataaaat attcataaaa tcactatgaa ttattaacta ccttgtcaaa aatagatttg
     3541 tattttttag tcacggtatt ccactgaaaa ttttcatgtg aacgcctttg ggcgttctca
     3601 ccgaacaata tccttttatt ctcatccact aacaaatcat gtatgctttg atataattca
     3661 tcgatgttgt ttataatcac gcctgtagaa ttattgatta ctgcctctgg agtgcctcct
     3721 acattagaag ctatagaggg aataccaaaa aaagccgctt ctaaatagga aataccaaat
     3781 ccttcaattg atctactttt gctttcatcc aaggtaggca ttaccattaa acttgatctt
     3841 tcaaataaaa attttttttg ttcatcatta accaaaccaa caaataatac attgttttgc
     3901 aaatttttat catttacaag tttttgcaag gcgcgctttt ctggaccttc acctgcaata
     3961 acatattgaa tgttgggaaa atcagataat aattttttga tgcaatggat gatgtgggcg
     4021 tggctttttc ttttttctaa tcttgctaaa gttaatataa caggctcgcc atttacttcc
     4081 tttaaagagt gctctttatg atttcttaag tcattcgccc cgggataaac ataatcaaca
     4141 ataattgttg atttaactaa ttcttgaact aatttttttg tgtatagcga atttgcaaca
     4201 acggccgtac ttttgttaag tgtattctta atacgttgag cttttttaag atcattagat
     4261 aatagttcgt ttccatgagc taaacaaata gagggaatat tttttgtgtt aaaatagtca
     4321 atacccaatt ccaagctctt ccaggtatca gctataatta gtttgacttt gtgtgattca
     4381 atcaaaagct taatttcttt aattttgcgt cgctgtcgaa aaaattttaa tccacttatt
     4441 cgacgaacaa gaatttcatc cttgtggttg ttatcgtata tagcgtcata aaacatatga
     4501 tggcgatcag caaaaacaat tattctttca ctcttgctca aacctacaga caaattacta
     4561 acaaggcttt ctattcctcc aagacgactt gggaagcatt gggttaaaat caaaatcatt
     4621 aatttattta attatatcga tttaaaatta aactatatac ctctaaatta atatgcatta
     4681 attgcatatc atacattcaa ttttgttgct tttattgcat atataaatac ctatatgaac
     4741 atctatggaa aggataaatc aataatgttt tttaaatcat tcattacaac tcttaatagt
     4801 aattataacg cttttaatga ttctgggttc gataacgttg acccaaacat cgttaaatat
     4861 ttccgaactg aatacgggaa agattggaag gttgcgcttc aacaccatct atacaaaaaa
     4921 agtgtaaatg gcaaaaaagc tgcttagtaa acttgaaaag ctagtttctg ccatcgcaaa
     4981 aagaggatca tcacataata catggtatgg attttcaaat aagtaccata aaaatcaagt
     5041 tgaaaaatac ttagcaaatt cttatgatct ctatgatcta gattatcgtc aaaaagagct
     5101 agacagaaaa ggcacctaca ataaacttta tattcaataa ttacttatta tcttttagag
     5161 gggcgtggtc accttagtca accattcctt ttcttgagat ttaagataag gacttaattt
     5221 attgtataca tcttggtgat atatatttat ccaatttatt tcattgtcta acagaatact
     5281 tttctcaatt agatccctgt ctattggggc ccaagatatt gtttcaaaaa acatagtatt
     5341 gttggattta atacaagcaa ttattaaatt ctctgtacga atgccgtatt catttatttt
     5401 atagtatcca ggttcattcg ataagatcat gccgggataa attattccat cgcttaatcc
     5461 ttgatttttt gcaattcttt gcggaccttc gtgaacactt aaaaaacttc caattccgtg
     5521 gcctgttcca tgatcgtaat cgcaccctat tgcctgaaga ctttcacgcg ccaaaggatc
     5581 tagggttgag ccaacactat tggatgcaaa ctcagctgtt gctatagcaa tatgaccctt
     5641 taaaactctt gtgaaacgat ctttttgttc attagtagct tttccaaata taacagtcct
     5701 agttatgtca gttgttccat ccctatattg cactccagag tcaacaagat aaatagagtt
     5761 attttcaaaa gacaaatttg tctctgggga tactctgtaa tggggcaggg ctgcatgcgg
     5821 tccaaatgcg gaaatagtat caaacgaagg agaataaaat aattcatttt ctttacgtaa
     5881 attcagtaag taatccgcgg cattaatttc atttgtttca ctgattttca ttatattttt
     5941 taaccagtac aaaaattttg taatacttgc accgtcacga atatttgcat ttcgagctcc
     6001 taataattca atttcgtttt tttgtgcttt tggatatgta caaggattag acaaataatt
     6061 aattgttata tcattgttca aacaaatttt tttaaaatta taaacagttt gtttttcatc
     6121 cattccaaca atcttatcct tacctatctt tcctataaat tcataaatac tttcaaaaga
     6181 atggaagtta acaaattttt ctaattcatt tttaatagat ctaatttttt tttcttctac
     6241 aaaaaattca acgttacctt tttgagggat aataacgaag caacaaagta agggggtgta
     6301 atcaatatca ttgccacgta tgtttaataa ccaagcaatg gagtctaaag aggttaaaat
     6361 ataataatca ataaatgttg aagccagaga attttgaatt tcatatattt tatccctgcc
     6421 tgccttacct acatatttaa agtcatgaat aaaggctttc gattgtgggt aaaaaggttg
     6481 ggttttccaa tatttatcta ttggattgtt gtctaaatat tgcaaactag attttctatt
     6541 atcaacaatc tgttgaattt tttctacctc ttcaacagaa tgcaatgttg gatctatagc
     6601 tataattttt ccctcgaaac ataactcctc taaaaagttc cagtaatctt gcagatgttt
     6661 cactaaaaaa aactctgaat cagtttgctc ttgggcttga atagaatagc gtccatccac
     6721 aaacaaatat gctttttctt ggaaaataat ggctttccca cttgatccag aaaaattact
     6781 tatccattgc aaacgctcag cataggaagc aatatattcg cctaaaaatt catcggtacg
     6841 attaattata aacacatcga tagagtgatc cttcatccaa gtttgaattt tttgtaaggg
     6901 gttcattatt tttcttttgc aatactagaa aaaagttcac catcaatgtt accgcctgaa
     6961 atcatcacaa ttattgtttt attttccaca tcaagtttct tatttaacaa agccgaagca
     7021 gctacagctc cgcccggttc tgtgataatt tttaaatttt ctgctagtac aacaattgta
     7081 cttttaacct ctttgtcgct tactgttaat ccatctgtga gtgtttgttg attaattgca
     7141 aatgtaatat caccaggttg cggtgcaaga agtgcatcgc atatggattg tgctcccttt
     7201 tttattgggg tgataaaatt tgatcggaat gacaattggg tatcgttaaa ttcctctggt
     7261 tcaactgaat aaatctcgat atttggaaac ttatgtttaa gataggtaga agtcccggca
     7321 attagtcctc ctcctccaca gcaacatagg tacatatcag gacggatatt ttgatctaac
     7381 agttcttctg ctatttcctt gcccgcagtg ccttgtccag cgataatatc cagatcatca
     7441 tagggtttaa taatagtttt ttttgttgtt cttgcaatat cattggctat atcctctcta
     7501 acaccacttg caggatcata taaaacaact tccgatccat atctcttggt attatttatt
     7561 ttaatagaag gggcattctt gggcataaca atagtcgatg aaatattatt tagttttgat
     7621 gcataagcta ccgcttgggc atgattccca gaggaatagg ctactactcc cttgtttttt
     7681 tcattttttg ttagttgacc aatcttattg ctcgctcctc tcaacttaaa
//
LOCUS       DTVV01000199            1437 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1522900, whole genome shotgun sequence.
ACCESSION   DTVV01000199 DTVV01000000
VERSION     DTVV01000199.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1522900"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
BASE COUNT          537 a          187 c          273 g          440 t
ORIGIN      
        1 ggagttacta atgcaaattg tggttataaa aacactccaa gaagatatca caccgcggga
       61 ttagaaacta gattttataa agatataaat tggtttggaa aacctaacaa tttaaaatat
      121 ggaataaagt ttgaatttga aaaaatagaa agaaaagccc gacaaactgg aagtgataaa
      181 aaacaaacag gtacccaaat ggctgaagat ggtacggaat ttgcaaaaat gagcgaagat
      241 gcggaagtat acgcacttgc tttgtattta gaagatgaca ttcaagcaac tgataaactt
      301 attatgacac ctggagtaag atacgaaagc tattatttga tacataatga tagagaaaga
      361 aaagatggtt gtggggcaac cggtgttacg gattgtaata attatacctc ttatgaaaaa
      421 gacgaataca ttcttttacc tggtttaggg tttacttatg aagcaaataa tacaaataaa
      481 atttatggag gtcttcacgt gggtatggcc ccacccgcgg ttggtgatgc tggttacaga
      541 caaatatcaa atttgaaatc tcaaaaaagt tttaattttg aattaggctt aattaataat
      601 agttttgaag ataaattggg tttaactttt gaaactgctg catttagaac agttgaaaga
      661 agtagacctg ttaaatcctc tctaagaact gctggtacag gctcaaaatt aaaaaacatt
      721 ggtacaactt ttacagatgg aattgaattt tctgcaaatt gggatcaatc aagatattct
      781 aaaaattcac gaaattggtt tgcaacacta agttatagtt tcatgtatcc aaaattaaaa
      841 acccatcaaa aaggggaaag atcaagttct gacgcaggca cgcctattgt tgtagatgat
      901 gtttatgaga atgatattcc ttttgtttca cgacataaag gactagtgac tgtgggatat
      961 ggaacaccta ataaatggaa tataagtaca actgctagat atcgtggaga atattttgct
     1021 gatatagata atacaaaagg tattggccaa agcggacgtt ggggccaagt cgatgatcat
     1081 tggataataa atgcaagagg aaattatact ttatcaagat ttaacaattc aactttgtat
     1141 ctaagtttaa ctaatatttt tgatacaact tatatcgcat ctgtatcagc agaaggttta
     1201 aaagtaggta acggtcgatc aattatgaca ggtattgaat ataacttcta aatatattgt
     1261 taaatcaatt aattaaaatt tttttttttt aagatttgcc atatttttgc caaagaaata
     1321 actatattaa atagtgtgtc ggaataataa tttcagacat gattattaac tttgtatagg
     1381 gagaatacac atgacttggt atacacctaa agtatcagaa gttgctgttg gcttaga
//
LOCUS       DTVV01000200            1604 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1527991, whole genome shotgun sequence.
ACCESSION   DTVV01000200 DTVV01000000
VERSION     DTVV01000200.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1527991"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="EYO86_02385"
     CDS             <1..730
                     /locus_tag="EYO86_02385"
                     /inference="COORDINATES: protein motif:HMM:PF00795.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIC41915.1"
                     /translation="NLLKTIPFNPDIISTPECSNIITSDRAHLFKYATYQSKCPVLDV
                     CFKFAKKYKKIVHIGSLLLKKKNSKKLLNRSFLINKEGKIIDYYDKIHLFDVNINSKE
                     IHRESQSFNKGKKIVVCKIKQVTLGLSICYDIRFPALFRDLTKKGAQIIFIPSAFTVP
                     TGKDHWETLLRTRAIENTVFVVATAQCGRHHNNRKTYGHSMIINPWGKIIRKTTSLPK
                     ILNSSINLEEIKVTRLRMPSIYHD"
     gene            complement(770..976)
                     /locus_tag="EYO86_02390"
     CDS             complement(770..976)
                     /locus_tag="EYO86_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41916.1"
                     /translation="MNEDNINMEIRKFLKKVGITSQRIIENQINMANKSGSIKIGDEI
                     ELEMSLSIKNFNSINSISGKILID"
     gene            1161..>1604
                     /locus_tag="EYO86_02395"
     CDS             1161..>1604
                     /locus_tag="EYO86_02395"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:PF00275.18"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIC41917.1"
                     /translation="MDKIKIKGGANLFGSINIPGSKNAALPVMVSSLLSKENLNLHNL
                     PKLQDMNSMISLLRSFGVSVNQNNHTLTLNSQNLKNSTASYDLVRKMRASILVLGPLL
                     SRFREAKISLPGGCAIGTRPIDIHLDGLSKLGVDFEIDNGFVKGRV"
BASE COUNT          589 a          235 c          241 g          539 t
ORIGIN      
        1 aaatttatta aaaacaattc cttttaatcc agatattatt tcaacgcctg agtgttctaa
       61 tattattact agtgatagag cacatctttt taaatatgca acttatcaat caaagtgccc
      121 ggtgctggat gtatgcttta agtttgcaaa aaaatataag aaaattgttc atatagggtc
      181 tctattgtta aaaaagaaaa actccaaaaa acttttaaac cgttctttct taattaataa
      241 ggagggtaaa attattgatt attacgataa aattcattta tttgatgtca atattaattc
      301 aaaagaaata cacagagaat cccaatcatt taataaagga aaaaagatag tagtttgtaa
      361 aatcaaacaa gtaactttag gcctatcaat ttgttacgat atacgttttc ctgcattgtt
      421 tcgcgattta actaaaaagg gagcgcaaat aatttttatt ccctcagcat ttactgtacc
      481 aacaggaaag gatcattggg aaacattgct tcgaacacgt gccattgaaa ataccgtgtt
      541 tgttgtggca acagctcaat gcggtagaca tcacaataat cgcaaaacct acggtcattc
      601 aatgatcatt aatccatggg gaaaaataat cagaaaaaca acatctttac ctaagatttt
      661 gaactcaagc attaatctag aagagattaa agttactcgt ttgcggatgc cttcaattta
      721 tcatgattga agttattaaa ctaagtttaa agatggcaat aattatttac tagtcgatta
      781 atattttgcc gctaattgag tttatggaat taaaattctt aatagacaaa gacatttcta
      841 attctatttc atcaccaatc ttaatagagc cactcttatt tgccatattg atttggtttt
      901 caattattct ttgtgatgta atgccaactt tttttaagaa ttttcttatt tccatattta
      961 tgttatcttc attcatgcta ttcctttctg ggttccattt tatcattttt caacctattc
     1021 gcaaaaaaaa tatatgaata aaaaaaccac aatttcaact aaatcttttc ataaaaaaag
     1081 gttgaaagaa ttaagattaa aaaaattaga acaaaaaatg aaactaaata ttcttaaaag
     1141 aaaaaaaatt agtaataaag atggataaaa tcaagatcaa gggaggcgct aacttattcg
     1201 gttcaattaa tataccggga tcaaaaaatg ccgctcttcc agtgatggtg tcctcacttt
     1261 taagtaagga aaatttaaat ttacataatt tgccaaaact gcaggatatg aactctatga
     1321 taagtttatt aagaagtttt ggtgtatcag taaatcaaaa caatcataca ctaactttaa
     1381 attctcaaaa tttaaaaaac agtactgcta gttatgattt ggttcgaaaa atgagagctt
     1441 caattttggt cttaggaccc ctactttctc gttttaggga agcaaagatt tctcttccag
     1501 gggggtgtgc aattggtact cgaccaatag atattcattt agatggatta agtaaattgg
     1561 gtgttgattt tgaaatagat aacggattcg taaagggcag agtt
//
LOCUS       DTVV01000201            1732 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1530497, whole genome shotgun sequence.
ACCESSION   DTVV01000201 DTVV01000000
VERSION     DTVV01000201.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1530497"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="EYO86_02400"
     CDS             complement(<1..136)
                     /locus_tag="EYO86_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41918.1"
                     /translation="MKRNRKAKILATLGPSSSSPEVIEALFNEGCDVFRLNFSHGSIE
                     D"
     misc_feature    complement(141..197)
                     /note="possible 23S ribosomal RNA but 16S or 23S rRNA
                     prediction is too short"
     gene            199..483
                     /locus_tag="EYO86_02405"
     CDS             199..483
                     /locus_tag="EYO86_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HIC41919.1"
                     /translation="MNKTIQHELYAEVLQTLIEHLRNRNDVQNIDLMNLSGFCRNCLS
                     KWYVKAAEKRNIAVNYEEAREIIYGMPYSEWKAKYQTEATKEQLEKYKSE"
     gene            complement(487..>1732)
                     /locus_tag="EYO86_02410"
     CDS             complement(487..>1732)
                     /locus_tag="EYO86_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIC41920.1"
                     /translation="TACIIYTSGTGGSPKGVMLSHGAMLTNCSGAQALLKNLISDLDE
                     IRFLSWLPLSHSYEHTLQFYEMGIGAQIYYAESIDKLLINMDEAKPHFMTAVPRFYDS
                     LYTRISQGLKNQGKISQILFAATLRLGKKQYYNSKMTVLEKIFNNILKKVVRKKVNKR
                     FGGSLRALISGGAALNFEVGLYLTALGLPLLQGYGQTETAPVVSANPPEKIKLDTVGT
                     IFKGTRVKIANDGEILVSGENIMNGYWNDPEATASTIIDGWVHTGDIGEFDDDQYLKI
                     TDRKKDIIVNAGGDNISPSRVEEKLNIEPEIAQSMMYGDFKNYLVAIIVPDKDLALQW
                     ANDHGKKPDLASLIKDDDFIKMMKDVTVKVNNNLSAIEQVRKFLLVEEEFTIENDMMT
                     PTMKVKRFKVKKKYIDQLENLY"
BASE COUNT          569 a          301 c          275 g          587 t
ORIGIN      
        1 gatcttcaat agatccgtgg gaaaaattta atctgaaaac atcacaacct tcattaaaaa
       61 gagcctcaat aacttctggg gatgatgaag atggtccaag ggtagctaaa attttagctt
      121 ttctgttacg tttcattaga tttattgtat ataatttaaa ataaacaaat atgtaactta
      181 tattgtgaac ttttttatat gaacaaaaca atacaacatg aattatatgc tgaagtgttg
      241 cagactctaa ttgagcattt acgaaatcgt aatgatgttc aaaatattga tttgatgaac
      301 ctttctggat tttgtcgaaa ttgtttgtcc aaatggtatg taaaagctgc ggaaaaaaga
      361 aatattgccg ttaattatga ggaagcaaga gaaattattt acggtatgcc atattctgaa
      421 tggaaggcaa aataccaaac agaagcaaca aaagaacaac tagaaaaata caaaagcgaa
      481 taagtattaa taaagatttt ctaattgatc tatatatttt ttctttacct taaatctttt
      541 tactttcatg gttggagtca tcatgtcatt ttcaatggta aattcctctt caactaaaag
      601 gaattttcga acttgttcaa ttgcggataa attattgttg acttttacag ttacgtcttt
      661 catcattttt ataaaatcgt catctttgat aagcgatgct aaatcaggtt tttttccatg
      721 atcgttcgcc cactgcaagg ctagatcttt atcgggtaca attattgcaa caagatagtt
      781 tttaaaatca ccatacatca tcgattgtgc aatctcaggt tcaatattaa gtttttcttc
      841 aacacgagag ggggaaatat tgtcaccacc tgcatttaca ataatatctt tttttctatc
      901 tgtaattttt agatattgat catcatcaaa ctcaccaata tcacctgtat gaacccagcc
      961 atctataatt gtggatgctg tagcttcagg atcattccaa tatccattca taatattttc
     1021 accgctaacc aatatttctc catcgttagc aatcttaacc cgagtaccct tgaaaattgt
     1081 acctacggta tcaagtttaa ttttttctgg tgggtttgca ctaaccaccg gggcggtttc
     1141 tgtttgacca tatccctgca acaaaggcag acccaatgca gttaaatata atcccacttc
     1201 aaaattaagg gctgcaccac ctgaaattaa agctcgtaaa ctacctccaa aacgcttatt
     1261 cactttttta cgaactactt ttttaagaat gttgttaaaa attttctcaa gaactgtcat
     1321 ttttgaatta taatattgtt tttttccaag acgtaatgtt gcagcgaaaa gtatttgact
     1381 aattttacct tggtttttta agccttgaga aatacgagtg taaagagagt cataaaaacg
     1441 aggaactgct gtcataaaat gtggctttgc ttcgtccata ttgatcaata atttatctat
     1501 actttcagcg taatatattt gggcaccaat acccatttca tagaattgta gtgtgtgttc
     1561 atatgaatgt gaaagaggca accaagataa aaaacgaatt tcatccagat cagaaattaa
     1621 atttttgagt agggcttgtg ccccagaaca gttagttagc attgccccat gactaagcat
     1681 tacccctttg ggacttcctc cagttcccga agtatatata atgcaggctg ta
//
LOCUS       DTVV01000202            1455 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_153354, whole genome shotgun sequence.
ACCESSION   DTVV01000202 DTVV01000000
VERSION     DTVV01000202.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_153354"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="EYO86_02415"
     CDS             complement(<1..989)
                     /locus_tag="EYO86_02415"
                     /inference="COORDINATES: protein motif:HMM:PF12708.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41921.1"
                     /translation="MIRHNLTPFLFSIIVLMQCEISASENEGISLKIIGDSTIYLPDY
                     SFAGYHHGEVSIPNYTRSYNVSDYGAIPNDEKDDTQGIITAIAEASKEKGTTVVKFSP
                     GKFILKKIIFIERSNFVLQGSGSDSNGTIIYCPTPLRDLPLPKEMEELNQYLLENNKR
                     DKKTGKIFSPFSWTGGFIWVQKKESRPHRYLATFDKTELQLASVDNGKRGSNYFTVKN
                     VKSINENLLAKLLWFNRSGPQSSLLRHMYGDGDFFIGSHHWNYPERALIEQILTIKKI
                     QNDRIYIIEPLLHDVKNDWGTILAPADLLTEVGIEKLRIEFPQTNYGGHHLEEG"
     gene            complement(1010..>1455)
                     /locus_tag="EYO86_02420"
     CDS             complement(1010..>1455)
                     /locus_tag="EYO86_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC41922.1"
                     /translation="EKDWDDVMNLNSKSLFFLSQAVAQDMMKRGYGKIINIASILTFT
                     GGLTVPSYSASKGAVGQITKALANEWAYKGICVNAIVPGYMETNNTKPLREDPKRFQE
                     ISDRIPTGRWGKPDDLKGAVIFLASNASDYVNGHLLAVDGGWMVR"
BASE COUNT          432 a          292 c          214 g          517 t
ORIGIN      
        1 ccttcctcca agtgatgccc cccataattt gtttgaggaa attcaattct taatttttct
       61 attcctactt cagtcagtaa atcggcaggt gctaatattg tgccccaatc atttttaaca
      121 tcatgtaata atggttctat aatatatatt cgatcattct ggattttttt aatagttaaa
      181 atttgttcta taagtgcccg ctctggataa ttccaatggt ggcttccaat aaaaaaatcc
      241 ccatctccat acatatgtcg taataaagat gattggggac cacttctgtt aaaccataat
      301 aatttcgcta ataaattttc atttatcgac tttacatttt ttacagtaaa atagtttgac
      361 ccacgtttac cattatcaac tgaagctaat tgcaactcag ttttatcaaa tgttgctaaa
      421 tatctatgtg gacgcgattc tttcttttga acccaaataa aaccaccagt ccaagaaaac
      481 ggggaaaata ttttacctgt ttttttatct cgtttattat tttctaataa gtattgattc
      541 aactcttcca tttcttttgg aagtggcaaa tctctaaggg gtgttggaca ataaataata
      601 gtaccattgg aatcgcttcc agagccttgt aaaacaaaat tacttcgttc tataaaaatt
      661 atttttttta atatgaattt gcctggtgag aatttaacaa cagtagtccc cttttcttta
      721 cttgcttcag caattgctgt aattattcct tgagtgtcat ctttttcatc gttcggtata
      781 gcaccataat cactaacgtt gtatgatcta gtatagtttg gtatactgac ttccccatga
      841 tgatatccgg caaaggaata atctggtaaa taaattgtag agtcaccaat aattttaagg
      901 gaaattcctt cattctcact ggctgaaatt tcacattgca tcaatacaat gatggaaaaa
      961 agaaaagggg tcaaattgtg tcttatcatc aatctagcat ctcctgattc tatcttacca
     1021 tccagccgcc atcgacggcc aagagatgcc cattgacata atcagacgca ttcgatgcta
     1081 aaaaaatcac agcccctttt agatcatcag gctttcccca gcgaccagtt ggtatacgat
     1141 cacttatttc ctggaatctc tttgggtctt ctcgaagagg ttttgtattg tttgtttcca
     1201 tataaccagg cacgattgca ttgacacata ttcctttata tgcccattca ttagccaaag
     1261 cttttgttat ttgacctact gcacccttac ttgctgaata tgatggtaca gtcaatcctc
     1321 ctgtaaaggt taatattgaa gcaatattta ttattttgcc atatcctctt ttcatcatgt
     1381 cttgagcaac agcctgactc agaaaaaata aactttttga atttaaattc attacgtcat
     1441 cccaatcctt ttcag
//
LOCUS       DTVV01000203            1012 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1543143, whole genome shotgun sequence.
ACCESSION   DTVV01000203 DTVV01000000
VERSION     DTVV01000203.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1543143"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="nth"
                     /locus_tag="EYO86_02425"
     CDS             <1..618
                     /gene="nth"
                     /locus_tag="EYO86_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIC41923.1"
                     /translation="NRLYQKLAKHLSEAKSELVFKNHYELLISVILSAQSTDKTVNKV
                     TSALFSSCPTPAKMLALGQSKLAKIIKPIGLAPAKSKNIINTCKILLEKHNGRVPGNR
                     KDLENLPGVGRKTANVVLNEGFGKPTIAVDTHVFRVANRTGLAPGNSVLETEQKLLEV
                     TPKKWLKSAHRYLILHGRYTCTAKNFNCQKCVVKQECGFTEKLNS"
     gene            627..>1012
                     /locus_tag="EYO86_02430"
     CDS             627..>1012
                     /locus_tag="EYO86_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HIC41924.1"
                     /translation="MNSKKNDIKTIYSRLRKHLPGVNGFALNHLIETPEKMYSLVKFI
                     LGEGNRLKAMISAGIHGDEPSGVETICSFLENNRFEKFADVWELTFLPCLNPYGFEIG
                     SRENHEGKDLNRLFKHDSPPQEVKFA"
BASE COUNT          348 a          194 c          196 g          274 t
ORIGIN      
        1 aatcgcctat accaaaagct tgccaagcat ttatcagagg ctaaatcgga actggtcttc
       61 aaaaatcatt atgagcttct tatatcagta attttaagtg ctcaatctac agataaaacg
      121 gtaaataaag ttacatccgc tcttttctcc tcgtgcccta ccccagctaa aatgctagcc
      181 ttggggcaat ccaaactcgc aaaaattatc aaacctatag gcttggcccc tgccaaatct
      241 aaaaatatta taaatacctg taaaatttta ttggagaaac ataatggacg ggttcctggc
      301 aaccggaaag acttggaaaa tttgcctggc gtaggacgta aaacagctaa tgtggtattg
      361 aatgagggat ttggaaaacc tacaatcgct gtagataccc atgtttttcg agtagcaaac
      421 cgcacgggat tagcacccgg aaactctgtc ttagaaacgg aacaaaaact tttagaggtg
      481 acacctaaga aatggttgaa aagtgcccac cgttatctca ttcttcatgg aagatatacc
      541 tgcacggcta agaattttaa ctgccaaaaa tgcgttgtta agcaggaatg tggttttacc
      601 gaaaagttga acagttaaaa taaaacatga attccaaaaa aaatgatatt aagacaattt
      661 attccagatt acgcaaacat ttgcctgggg taaatggatt tgccttaaac catcttatag
      721 aaactccgga aaagatgtat tccttagtta aatttatttt gggagaagga aatcgactca
      781 aggcaatgat atctgcagga attcatggcg atgaaccaag tggtgtggaa acaatttgct
      841 cattcctgga aaacaaccgc tttgaaaaat ttgctgatgt atgggagtta acctttttgc
      901 cctgcttaaa cccctatggt tttgaaatag gatcaagaga aaaccatgaa ggcaaagact
      961 tgaatcgatt atttaaacat gactcacccc cgcaggaagt taagttcgca at
//
LOCUS       DTVV01000204            2772 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1543387, whole genome shotgun sequence.
ACCESSION   DTVV01000204 DTVV01000000
VERSION     DTVV01000204.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1543387"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            288..719
                     /locus_tag="EYO86_02435"
     CDS             288..719
                     /locus_tag="EYO86_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41925.1"
                     /translation="MIENFNGPIYLILFIILLLGNVFYAYCTLINTKNWLDKYGTHHS
                     AVLITRILGSLISGFVLIGIYILFTGTEGTWAYFATMFIGFVIMSIIGIHFVEVDYPN
                     NYKGKEGFENVVITKEGYIPAIIFAIINAIIIYGLSDKIYA"
     gene            complement(723..1460)
                     /gene="truA"
                     /locus_tag="EYO86_02440"
     CDS             complement(723..1460)
                     /gene="truA"
                     /locus_tag="EYO86_02440"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIC41926.1"
                     /translation="MPKFKITVEYDGTEFVGWQRQKNGPSIQSSIEDAIKKITSETVN
                     VFGAGRTDSGVHAKGQVAHFELSNKISLDSIRDGINQHLRPLPIAILDAKEVNGDFHA
                     RFSAQLRSYEYLIINRRSPLTLYKNQAWGVFKQLNINAMKKVAINFEGKHDFNGFRSI
                     DCQASSSIKTIQSCTVKKNKQYIVINVAAKSFLHSQVRIITGTLVDVGKGKISPTNIK
                     KIIESKDRSKAGTTAPAHGLYLLKVEY"
     gene            complement(1453..2379)
                     /locus_tag="EYO86_02445"
     CDS             complement(1453..2379)
                     /locus_tag="EYO86_02445"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIC41927.1"
                     /translation="MFKERVIFMGTPEIASVYLQSLIDSHFNIVAVYTQPPRKKGRGM
                     QVQESSVQKLAQKHKIEVYCPVDLDFSAAQKELQDLHPDIIVVMGYGLLLPKFILQLP
                     RFGCINIHVSLLPRWRGAAPIEHAILNGDKITGLTIFKLIEKLDAGPIIARDSIAIDP
                     HIDKNDLITLLNSKGTKLLISTLPDLFNKNVEFEIQKEDMATHASKITTEMRKLDFNQ
                     DALTVYNRIRAFAPQPSAWFVFNKERIKIIKAKLREGACEPSTIVNDQFHIGCNNGII
                     CPTIVQREGKKPMKIDEFLRGFDFAVGQKINA"
     gene            complement(2379..>2772)
                     /gene="def"
                     /locus_tag="EYO86_02450"
     CDS             complement(2379..>2772)
                     /gene="def"
                     /locus_tag="EYO86_02450"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIC41928.1"
                     /translation="PGVGLAANQIGILKKIVTVRIHDEEKKIDNTYVLFNPKIKSYSK
                     EKIIMEEGCLSLPKQYADIERPKSITVEYVNEKNEIIKEKKNGFEARVLQHEIDHLEG
                     KLFIDYLSSLKRNIFIKRVKKLKKLGEI"
BASE COUNT          849 a          469 c          416 g         1038 t
ORIGIN      
        1 caagatttct tggcagagga ttaggctttt ctgtacttgc cataactttg gtttttattg
       61 caacattatt tcaatgggga agattcacat ctttttttga aatttacaca gtcttatttt
      121 tgtttacgtt atttggtttt ttaaaccaac ttctcatgta tttaaaagtt ttaaaaactc
      181 ataatgataa gcctgtgcca atggcaaata taattagacc attaattccg ttagtagtaa
      241 taattattcg cagattaacc tcaactaact tttaactagg ggattatatg attgaaaatt
      301 ttaatggacc catatactta attcttttca taattttgtt gttgggaaat gtattttacg
      361 cttactgcac cttgattaat accaaaaatt ggcttgataa atatggtaca catcattctg
      421 cagttttaat aacaaggatt ctaggatctt taatttcagg ctttgtatta attggtattt
      481 atatattatt tactggaact gagggtactt gggcatattt tgcaacaatg tttattggtt
      541 ttgtaataat gtccattatt ggtattcatt ttgtagaagt tgattatcct aataattaca
      601 aaggcaaaga ggggtttgaa aacgtagtaa taactaaaga aggttatata cctgctataa
      661 tatttgcaat tataaatgca attataatat atggattatc agataaaatt tacgcttaat
      721 aactaatatt ctacttttaa taaatataaa ccatgagcag gtgctgtcgt acctgctttt
      781 gatctatctt tgctctcgat gatttttttt atattagtgg gtgaaatttt tcctttacca
      841 acatcaacta aagttccagt tattattcta acctgtgaat gaagaaatga ttttgcagct
      901 acattaataa caatatattg cttgtttttt tttactgtac aactttgtat agttttaatt
      961 gatgaagaag cctgacaatc aattgatctg aatccattaa aatcatgttt tccttcaaag
     1021 tttattgcga ccttcttcat agcattaata tttaattgct taaaaacacc ccaggcttga
     1081 tttttatata gtgttaatgg tgaacgtcta tttataatta aatattcata acttcgtaat
     1141 tgcgcagaaa atcgagcatg aaaatctcca ttaacttctt tcgcatctaa aatagctatt
     1201 ggtagcggtc ttaaatgctg attgataccg tcacgaatgc tatccaatga aattttatta
     1261 gaaagttcaa aatgcgctac ttgaccttta gcatgcacgc cagaatctgt tctgcctgct
     1321 ccaaatacat ttacagtttc agatgttatt ttttttatag catcttcaat tgaagattga
     1381 atagaagggc cattcttttg cctttgccac ccaacaaatt cagtaccgtc atattctact
     1441 gtaattttaa acttaggcat ttattttttg tccaactgca aaatcaaacc ctcttaaaaa
     1501 ctcatctatt ttcattggct tttttccttc acgttgaaca atagttgggc aaattattcc
     1561 attattacaa ccaatgtgga attgatcatt cacaatagtt gaaggctcgc atgctccctc
     1621 cctcaatttc gctttgatta tttttattcg ctccttatta aaaacaaacc atgcagaagg
     1681 ttgaggtgca aatgctctaa ttcggttata aactgtgagt gcatcttgat taaaatctaa
     1741 ttttctcatt tcggtagtaa ttttactagc atgggtagcc atgtcttcct tttgaatctc
     1801 aaactcaaca tttttgttaa ataaatctgg caatgtggaa attagcaact ttgtgccttt
     1861 ggagtttaat aatgttatta aatcattttt atcaatatgt ggatcaatag ctatactgtc
     1921 tcttgcaata ataggtcctg cgtctagttt ctctataagt ttaaaaattg tcagtccagt
     1981 aattttatca ccattaagta tggcatgctc aattggagca gcgcctcgcc accgcggtaa
     2041 aagtgaaacg tgtatgttaa tgcatccaaa acgtggaagc tgaagaataa actttggcaa
     2101 taacaaacca taccccatga ccactattat atcaggatgt aaatcctgca gttctttttg
     2161 ggctgctgaa aaatctagat ctacaggaca ataaacctct attttgtgtt tttgggctaa
     2221 tttttgcacg gatgattctt gaacctgcat tcctcgtccc tttttgcgag gcggctgcgt
     2281 ataaacagca acaatgttaa agtgtgaatc aattaaggat tgcaaataaa ctgaagcgat
     2341 ttctggagtg cccataaaga taacccgctc tttaaacatt atatttctcc tagttttttt
     2401 aattttttga cacgctttat aaaaatattc cgtttaagag aagataaata atctataaat
     2461 aactttcctt ccaaatgatc aatttcatgc tgaagaactc gtgcctcaaa tccatttttt
     2521 ttctctttaa taatttcgtt tttttcattt acatattcta ctgtaattga tttcggacgt
     2581 tcaatatccg catactgctt tggcagggac aaacatccct cctccataat tatcttctct
     2641 ttggaatatg atttaatttt aggattaaaa agaacatatg tgttatcaat ttttttttct
     2701 tcatcgtgga tacgaacagt aacaattttt tttaaaattc ctatttgatt agcagccaat
     2761 ccaacacctg gg
//
LOCUS       DTVV01000205            3023 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1546642, whole genome shotgun sequence.
ACCESSION   DTVV01000205 DTVV01000000
VERSION     DTVV01000205.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1546642"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>3023
                     /gene="rpoC"
                     /locus_tag="EYO86_02455"
     CDS             <1..>3023
                     /gene="rpoC"
                     /locus_tag="EYO86_02455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIC41929.1"
                     /translation="IRSWSFGEIKKPETINYRTFKPEKDGLFCARIFGPVKDYECLCG
                     KYKRMKFRGIICEKCGVEVTLSKVRRDRMGHIELAAPVSHIWFMKSLPSRIATLLDMT
                     IKNLEKVLYFEQFIVVEPGLTDLKKGEIISEDQYIDYQDQYGEDSFSAAIGAEAIEQM
                     LKNIDFEVDYEQLKLELTETKSELKKTKITKRIKLIENFISSKNKPEWMILRVLPVIP
                     PELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLIDLRAPDIIIRNEKRMLQESVD
                     ALFDNGRRGRVITSTNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLK
                     LHQCGLPKTMALELFKPFIFHKLDIYGWANTIKAAKKLVEKKDPRVWDILEEVIREHP
                     VLLNRAPTLHRLGIQAFEPILIEGKSIQLHPLVCTAFNADFDGDQMAVHIPLSLEAQL
                     EARVLMMSTNNILSPSSGKPIIVPSQDIVLGIYYLSIMRENQVGQGRHFLDLNEITKA
                     LENKVITLHTKIISRVKDFNNNYNRIETTAGRMILGNALPLNPHIKFELLNKVLTKKE
                     VSNIIDTVYRFCGQKETVLFADNIMRIGFKYAAKAGISFGKDDLIIPAEKKDLLDKTQ
                     ATVQEYENQYQDGLITQGEKYNKVVDAWSDCTNQVADKMLETISKTEDNKPINSVYMM
                     AHSGARGSAAQLKQLAGMRGLMAKPSGEIIENPIKSNFKEGLSVLEYFTSTHGARKGL
                     ADTALKTASSGYLTRRLVDVAQDAVIREDDCETIKGVLVEEIIESGNITSPLEDRILG
                     RCPVNDIKDAEDNIIVKSGDIIEETHLDEIKKLEFRSLKIRSVLTCKTLNGICAKCYG
                     RDLARGTAVNIGEAVGIIAAQSIGEPGTQLTMRTFHIGGAVSASVEQSSVSSPIDGVV
                     RTDDLKYIVDQNGNKILLTRNAKIYIYSNNEEKFSLNIPFGSKILIENNQNVKANQLL
                     ADWDPFTLPIIAEKNGFIKYLDLKQGISFRDTVDD"
BASE COUNT         1119 a          411 c          568 g          925 t
ORIGIN      
        1 atataagatc ttggtctttt ggagaaataa aaaaacccga aacaattaac tatagaacat
       61 tcaaacctga aaaggatggt ttgttttgtg ctagaatttt tggacctgta aaggactatg
      121 aatgtttatg tggcaaatac aaacggatga aatttagggg tattatttgt gaaaaatgtg
      181 gtgtggaagt cactctttct aaagttcgaa gagatcgaat ggggcatata gaattagcag
      241 cacctgtatc ccatatttgg tttatgaaat cattgccaag tagaattgca accttacttg
      301 atatgacaat aaagaacttg gaaaaagttc tctattttga acaatttatt gttgttgaac
      361 ctggtttaac tgatttaaaa aagggtgaaa taatatctga agatcaatat attgattatc
      421 aggatcaata cggagaagat tcatttagtg cagcaatagg agccgaagct attgagcaga
      481 tgttaaagaa catagatttt gaagtagatt atgagcaact aaaactagaa ttgactgaaa
      541 caaaatcaga attaaagaaa accaaaatta ctaaaagaat aaagttaatt gaaaatttta
      601 tttcttctaa aaataaacca gaatggatga tattaagagt gttgccagta attcctcctg
      661 aattaagacc tcttgtgcct cttgatggag gcagattcgc tactagtgat ttaaatgatt
      721 tatatagaag agtaattaat agaaacaata gacttaaaag acttattgat ctccgagctc
      781 ctgacataat tataagaaat gaaaaaagaa tgcttcaaga atcagttgat gcattatttg
      841 acaatgggcg acgaggtaga gttataactt cgactaacaa acgtccttta aaatctttgt
      901 ctgatatgct caagggtaaa caaggacgct ttagacaaaa cttattgggg aaacgagttg
      961 actattctgg tcgatcagtc atagttgttg gaccaaattt aaaacttcat caatgtggat
     1021 tgccaaaaac gatggctctt gaattattta aaccatttat ttttcataag ttagatatat
     1081 atgggtgggc caatactatt aaagctgcaa aaaaattagt agaaaaaaaa gatcctagag
     1141 tttgggatat tcttgaagaa gttataagag agcatcctgt attattaaat agagcaccta
     1201 cattgcatag acttggtatt caggcatttg aaccgatttt aattgaagga aaatcgattc
     1261 aattacaccc actagtatgt acagctttta atgccgattt tgatggagat caaatggctg
     1321 tacacattcc tttgagtctt gaggcacaac ttgaggctag agtattaatg atgagtacta
     1381 ataatatatt atctccatct agcggtaaac ccataattgt tccttctcag gatattgttt
     1441 tgggcattta ttatttatca ataatgagag aaaatcaagt tggtcaaggc agacattttt
     1501 tagatttaaa tgagattaca aaagctttag aaaataaagt tataacactt catactaaaa
     1561 ttatttctag agttaaggac tttaacaata attataatag aatagaaact actgctggtc
     1621 gaatgatatt aggtaatgct cttccattga atccacacat taaatttgaa ttattaaata
     1681 aggttttaac aaaaaaagaa gttagcaata ttatcgatac tgtttatcgt ttttgcggtc
     1741 aaaaagaaac cgtgctattt gctgataata taatgagaat tggatttaag tatgctgcca
     1801 aggctggaat ttcatttggg aaagatgact taattattcc agccgaaaaa aaagatctac
     1861 ttgataaaac tcaagcaaca gttcaggaat acgaaaatca atatcaagac gggctcatca
     1921 ctcaaggtga aaaatataat aaagttgtag atgcgtggtc agattgtacc aatcaagttg
     1981 ctgataaaat gcttgaaact atatccaaga ccgaagataa caagccaata aattctgttt
     2041 atatgatggc acattcagga gctagaggat cagcagctca gttaaaacaa ttagccggta
     2101 tgcggggttt aatggctaaa ccttcagggg aaatcattga aaacccaatt aagtcaaatt
     2161 ttaaagaagg actatcagta ttagaatatt ttacttctac acatggagca agaaaaggac
     2221 ttgcggatac agcattaaag acagccagtt ccggatatct aactcgtaga cttgtagatg
     2281 ttgctcaaga tgcagtaata cgtgaagatg attgtgaaac tataaaaggc gtgcttgttg
     2341 aagaaatcat tgagtcggga aatatcacat ccccattaga agatagaatt ctaggtagat
     2401 gtccagttaa tgatattaaa gatgctgaag ataatattat tgtaaaaagt ggagacatta
     2461 ttgaagaaac tcatttagat gaaataaaaa agttagaatt tcgttctttg aaaattagat
     2521 cagttttaac gtgtaaaact cttaatggaa tatgtgcaaa atgttatgga agagatttag
     2581 ctagaggtac tgcagtaaat ataggtgaag ctgttggaat tattgcagct caatcgattg
     2641 gtgaacctgg tacacaactt acgatgagga catttcatat tggaggagca gttagcgctt
     2701 ctgttgaaca atcttcagta tcatcaccga ttgatggagt tgttcgaact gatgatctaa
     2761 aatatattgt ggatcagaat ggtaataaaa ttttgttaac aagaaatgca aaaatttata
     2821 tttattcaaa taatgaagaa aaattttcat taaatattcc ttttggatca aaaattttaa
     2881 tcgaaaataa ccaaaatgtt aaagctaatc aattgctcgc tgattgggat ccattcactc
     2941 ttccaattat tgcagaaaaa aatggattca tcaaatattt agatctcaaa cagggcatct
     3001 catttagaga cacagttgat gat
//
LOCUS       DTVV01000206            1179 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1548459, whole genome shotgun sequence.
ACCESSION   DTVV01000206 DTVV01000000
VERSION     DTVV01000206.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1548459"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="EYO86_02460"
     CDS             <1..567
                     /locus_tag="EYO86_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIC41930.1"
                     /translation="MKEDAVEVLNETFRNMETEAIKNFEKEKITKESITLKRFGSFRY
                     LGQDHSVEIPLRNENYNQSSIGEIISDFHNQYEKEFTYRLDSQVDMIQFHLVVFAKIE
                     KPKLQKRRKTGINIEKTVKEQRKVDFDEQGIHDSKIYDFNILEPDMEFSGPAIVEDPS
                     TTVVIFPGQKCKVDDYANLHITFTNGDK"
     gene            564..>1179
                     /locus_tag="EYO86_02465"
     CDS             564..>1179
                     /locus_tag="EYO86_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIC41931.1"
                     /translation="MKKNLDPITVEIIQSSLQAACDEMFVAMRKTAMSSIIYEVLDFG
                     TAVTDTNGNIAASGAGIPAFIAMCDKAAQAVIKKFDSKDIREGDIFATNDPYNGGVTH
                     LNDVIVVTPIFCGGERIAWSANIAHWPDLGGMAPGGISADATEIFQEGLQLPVIKMIE
                     QGKPIRSVIDIITANSRVPQYTLGDMWAAIASIRVGEKRIIDIAK"
BASE COUNT          438 a          157 c          235 g          349 t
ORIGIN      
        1 atgaaagaag atgcagtaga agtattaaat gaaacattca gaaacatgga aactgaagca
       61 attaaaaatt ttgaaaagga aaaaattact aaagaatcca taacattaaa aagatttggt
      121 agctttagat atcttggtca agatcattct gtagaaatac cattaagaaa tgaaaattat
      181 aatcaatcat caataggtga aataattagt gattttcata atcaatacga aaaggaattt
      241 acttatagat tagatagtca agttgatatg attcaattcc acttagtcgt gttcgctaaa
      301 attgagaaac ctaaacttca aaaaagacga aaaacaggaa tcaacattga gaaaactgta
      361 aaagagcaac gcaaagttga ttttgatgaa caaggaattc atgattcaaa aatatatgat
      421 tttaatattt tagagcccga tatggaattt tcaggtcctg caattgttga ggatccaagt
      481 acgacagttg taatatttcc tggtcaaaaa tgtaaagttg atgattacgc aaatttacat
      541 attactttta cgaatggcga taaatgaaga aaaatcttga tcctatcaca gttgaaataa
      601 tccagagttc tcttcaggca gcatgtgatg aaatgtttgt tgcaatgagg aaaacagcta
      661 tgagttcaat tatatatgaa gttcttgatt ttggtacagc tgtaactgat acaaatggaa
      721 atatagcagc ctcaggggct ggcatcccag cgtttattgc aatgtgtgat aaggcagctc
      781 aagccgtaat aaaaaaattt gatagtaaag atattagaga aggagatatt tttgctacta
      841 atgatccata caatgggggc gttacacatt taaatgatgt tattgttgta acgccaattt
      901 tttgtggtgg tgaaaggatt gcatggagtg caaatatcgc gcattggcca gatcttggag
      961 ggatggctcc aggagggata tctgctgatg ctacagaaat ttttcaagaa ggtcttcaat
     1021 tacctgtaat taaaatgatt gagcaaggca agccaatcag atcggtcatt gatattatta
     1081 cagctaatag ccgtgttcct caatatacct taggtgatat gtgggctgct atagcttcaa
     1141 taagagtcgg tgaaaaaaga attattgata ttgcaaaaa
//
LOCUS       DTVV01000207            1422 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1552055, whole genome shotgun sequence.
ACCESSION   DTVV01000207 DTVV01000000
VERSION     DTVV01000207.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1552055"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="EYO86_02470"
     CDS             complement(<1..954)
                     /locus_tag="EYO86_02470"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIC41932.1"
                     /translation="MKRINQIEPSMDNAEKKELMSVIDSGWYTESSKTKEFERMFASF
                     VGAKYACAVTSGTAALYVGLKALNIGMGDEVIVPDLTFVASPNSVEATGAKPVLVDIE
                     KNALNLDLNKMSKSVNKKTKAVMPVDFNGRPIDMKVLREFADKKDIMLIEDAAHAIGC
                     YYNRRHVGTISDVGIFSFSTPKIITTGQGGMIVTNDRQIYERAMALKDFGRKIGIKKQ
                     MEKSFSHSTIGYNFKFTEFQAAIGIAQMRKLQKRILKKRRMFMKYVDLLSNVEGIEFL
                     KTNLKNVTPWMIDIFLKSPAKRAQLIKYLEKKQIGTRIYYPP"
     gene            993..>1422
                     /locus_tag="EYO86_02475"
     CDS             993..>1422
                     /locus_tag="EYO86_02475"
                     /inference="COORDINATES: protein motif:HMM:PF01370.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIC41933.1"
                     /translation="MTYLLNMNKIKSILVTGGAGYIGSVLTHKLVELGYNVRIIDSLI
                     YGRDGISDLISKNSVELIEKDIRDEKTLNEAVKDIDCIIHLAAIVGDPFCKKIPVAAK
                     QINEDATKKLVNISKKQGVKRFIFASTCSNYGSASTIVDEN"
BASE COUNT          452 a          239 c          218 g          513 t
ORIGIN      
        1 gggtggataa tatattcttg ttccaatttg ttttttttca agatatttga ttaattgtgc
       61 tctttttgcc ggtgatttta gaaaaatatc tatcatccat ggtgttacgt tttttagatt
      121 agtttttaaa aattctatac cttcaacatt agaaagtaaa tcaacgtatt tcataaacat
      181 tcttctcttt tttaggatac gtttttgtaa tttacgcatt tgtgcgatgc caatagcagc
      241 ctgaaactct gtaaatttaa agttatagcc aatagttgaa tgtgaaaatg atttttccat
      301 ttgttttttt ataccaatct tgcgcccaaa gtctttcaaa gccattgctc tttcatagat
      361 ttgtctatcg ttagtaacta tcataccacc ctgtccagtt gtaatgattt tcggtgtact
      421 gaaggaaaaa attccaacat cgctaatagt acctacatgc ctcctattgt aataacaacc
      481 gatcgcgtga gccgcatctt caattagcat tatatctttt ttgtctgcaa actctcgaag
      541 aactttcata tctattggtc taccattaaa atccacaggc ataacagcct ttgttttttt
      601 attaactgat ttagacatct tattcaaatc taaattcagt gcattctttt caatatctac
      661 tagaacaggt tttgcaccag ttgcttcaac tgagtttggg gaagcaacaa acgttagatc
      721 tggaacaata acctcatctc ccattcctat attcaaggct ttgagtccta catacagtgc
      781 ggctgtacca ctagttactg cacaagcata ttttgcccca acaaaacttg cgaacattct
      841 ttcgaattcc tttgtttttg atgattcagt ataccaacca gaatctataa ctgacattaa
      901 ctcttttttt tctgcattat ccatagatgg ttcaatttga ttaattcttt tcaaatgttt
      961 tcatctaatt tcttaaaaaa tgataaataa agatgactta tcttttgaat atgaacaaga
     1021 tcaaatcaat acttgtaact ggaggggcag gatatattgg aagtgtttta acacacaaac
     1081 ttgtagaatt aggttataat gttaggatca tagattcatt aatttatggc agggacggaa
     1141 tttctgatct aatatctaag aattcagttg aattaattga gaaagatatt cgggatgaaa
     1201 aaaccttgaa tgaggcggta aaagacattg attgtataat tcatttggct gccattgttg
     1261 gtgatccatt ttgtaaaaaa attccagtag ctgctaaaca gattaatgaa gatgccacca
     1321 aaaaactagt aaatatttca aaaaaacaag gtgtaaaacg atttattttt gcatctacat
     1381 gtagtaatta tggttcagct tcaactatag tagatgaaaa ta
//
LOCUS       DTVV01000208            2540 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1557981, whole genome shotgun sequence.
ACCESSION   DTVV01000208 DTVV01000000
VERSION     DTVV01000208.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1557981"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="EYO86_02480"
     CDS             complement(<1..783)
                     /locus_tag="EYO86_02480"
                     /inference="COORDINATES: protein motif:HMM:PF00459.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIC41934.1"
                     /translation="MPKLTNQNLDKIKDKIIDIALFASNTIMQNLGSTAKIKNDGSPV
                     TTADIKSDQIITEKLSSIFTNFPIVSEEKTAPKNALGDKTHWLIDPLDGTKSFIEGSN
                     DFVVCIALINNRSPILGCIAHPPTQKVWIGGKNIGSYVKLPKSNIRKIYCRDIPVRGP
                     TIATSRHHVGPKLKEWLRKIKYYKTISKGSAIKFTLIAEGKLDLYPRTSPTYEWDSAA
                     GHAIVEGSGGKVIQLNNKELIYGRSNRMNPNFIAFGRSNWSKF"
     gene            921..1853
                     /locus_tag="EYO86_02485"
     CDS             921..1853
                     /locus_tag="EYO86_02485"
                     /inference="COORDINATES: protein
                     motif:HMM:PF08392.10,HMM:PF08541.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HIC41935.1"
                     /translation="MQITDIEFTLGSKKETIEQLGKYNQGWDIEKIKDKTGVHIRHLL
                     RANEDEKTLVINATKKLINRVGVHKIDGLIHVSQSAFSRLPTSACLIQDILKLPKSIM
                     AFDLVQGCSGFVYGLSVASSMMDQFSFRRVLIVCADTYSRYILEDDRTTRPIFSDGAA
                     AVLLENKGSGSIGPFSFITDGSGGDSLTLINKNGKENLFMDGKKILQFTMREVPIVFN
                     DLLIKSGLDKEDIDLFIFHQASAVVLRRLKSKLKVPDEKWFENINEKGNTTSATIPIA
                     IAEAKKNKIYKANMKIMLIGFGVGLSVAGCIVRS"
     gene            complement(1848..>2540)
                     /locus_tag="EYO86_02490"
     CDS             complement(1848..>2540)
                     /locus_tag="EYO86_02490"
                     /inference="COORDINATES: protein motif:HMM:PF07940.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41936.1"
                     /translation="PRETRRGCGALAATTTCRCHPSLHTTHMLPKYIDMITDQKTSGR
                     SCKSCGPGLINSEKWQKALSASAAHSTLVINDTNSSSAKNKRIPIINIKRSVLTGGEQ
                     IFASHNGYYNIFTAKHERTLQLDKRGTILKGIDSIISGPGLKFQIHFHLHPRATTQLS
                     RNNEKALISLPSGGWEFSIINNKNSFDLTTEKSIYIEDDETTANSQQIIISGNTSNTG
                     AKINWCLIKKYL"
BASE COUNT          791 a          378 c          465 g          906 t
ORIGIN      
        1 aaatttagac caatttgacc ttccaaaagc tataaaatta ggattcattc tatttgatct
       61 tccatatatc aactctttgt tattgagttg aataactttt cctccagatc cttcaacaat
      121 agcgtgccct gcagcactat cccactcata agtaggactt gttcttggat ataaatctag
      181 cttaccttct gcaataagag taaattttat ggcacttcct ttgcttatag ttttatagta
      241 tttaattttt cttagccatt ctttaagctt aggacccacg tgatgtctgc tagttgcaat
      301 tgtaggtcct ctaacgggga tgtccctgca ataaattttt ctaatatttg atttaggcaa
      361 cttgacatat gagccaatat tttttcctcc tatccatact ttttgagtgg gggggtgtgc
      421 tatacatcct aatatagggg atcgattatt tattaatgct atacatacta caaaatcgtt
      481 gcttccctct ataaaagatt ttgttccatc aagaggatca atgagccaat gagttttgtc
      541 acccaaagca tttttaggag cagttttttc ctcactaact attggaaaat tagtaaatat
      601 agagctaagt ttttctgtaa taatttgatc tgatttgata tcagcggttg ttactggact
      661 tccatcattt ttaattttag cagtggaacc taagttttgc ataatagtgt tcgaggcaaa
      721 taaagcaata tcaattattt tatcttttat tttatctaag ttttgatttg tcagtttcgg
      781 catcaattat ctctttgtaa gttttagatg aatttatagt taaattgcta tattttataa
      841 ttggatattt ttagtttaat gctactatct aatatttact ttgcaatcta taagtaaatt
      901 ttttttggaa taaaagagct atgcagatta ctgatataga attcactctg ggcagtaaga
      961 aagaaactat agagcaactt ggaaaataca atcaaggatg ggatattgaa aaaataaaag
     1021 ataagactgg tgttcatata cgacatttat tgcgagcaaa tgaagatgaa aaaactttag
     1081 tgattaacgc cactaaaaaa ctaataaaca gagtaggagt acataaaata gatggtctca
     1141 ttcatgtaag tcagtcagca ttttctaggc ttcctacttc tgcttgttta atacaagaca
     1201 tattaaagct tcctaaaagt atcatggcat ttgatctagt acaaggatgc tccggtttcg
     1261 tatatggctt gtcagtagct tcctctatga tggatcaatt ttcatttcga cgtgttttaa
     1321 ttgtttgtgc tgatacttat tcaaggtata ttttggagga cgatagaact acaagaccaa
     1381 tattctcaga cggagcggca gcagttttat tagaaaacaa gggttctggg tcaattggtc
     1441 ctttttcttt tattactgat ggtagtggag gagattctct cacattaata aataagaatg
     1501 gaaaagaaaa tttatttatg gacggtaaaa aaattcttca atttactatg cgagaagtac
     1561 caattgtttt taatgatttg ttaataaaat cgggtttgga taaagaagac atcgatttgt
     1621 ttatatttca tcaggctagt gctgttgtat taagaagatt aaaatctaaa ttaaaagtac
     1681 ctgatgaaaa gtggtttgaa aatataaatg aaaaaggaaa tactacatca gcaacgattc
     1741 ctatcgctat tgcagaagca aagaaaaata aaatatataa ggcaaacatg aagattatgc
     1801 taataggttt tggggtagga ttatctgtag caggttgtat agttagatca taaatatttt
     1861 tttattaaac accaatttat tttggctccg gtattagaag tgtttcctga aataattatt
     1921 tgttgtgagt ttgctgtggt ttcatcgtct tcaatataga ttgatttttc agtagttaaa
     1981 tcaaaagaat ttttgttatt tataatagaa aattcccaac ctccgctagg aagtgatatc
     2041 aaggctttct cattatttct ggaaagctgt gttgttgctc ttggatgtaa atgaaaatga
     2101 atttggaatt ttaaaccagg acctgaaata atggaatcta ttccttttaa aattgttccc
     2161 cttttatcta attgaagagt tctttcgtgc tttgctgtaa aaatattgta ataaccgtta
     2221 tgactcgcaa aaatttgctc gcctccggtt aaaacagatc ttttaatatt tattatgggg
     2281 atacgtttgt ttttagcaga cgaagaatta gtgtcattta taactaatgt tgaatgtgca
     2341 gcagaggcag ataatgcttt ttgccatttt tcgctattta ttaaaccagg accacaactc
     2401 ttgcaggagc ggccggaagt tttctggtct gtgatcatgt caatgtactt cggcaacata
     2461 tgggttgtat gaagggaggg atggcaacgg caagtcgttg ttgctgcaag ggctccacaa
     2521 ccgcggcgtg tctcgcgcgg
//
LOCUS       DTVV01000209            1359 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1557984, whole genome shotgun sequence.
ACCESSION   DTVV01000209 DTVV01000000
VERSION     DTVV01000209.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1557984"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="EYO86_02495"
     CDS             <1..521
                     /locus_tag="EYO86_02495"
                     /inference="COORDINATES: protein motif:HMM:PF13406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIC41937.1"
                     /translation="VDSDQLQGSWGGAVGMTQMIPTTFLESGYDWDGNGVDIWNSYED
                     AFASAANYLTSLNKNPWLIHSTWGREVQPPSNIDSFYDDLKQDNPKGCGAVKSRSIPK
                     SLLEWSELGFLDINGNKLPSRQNLEARLIAPDGLKGRIFLVYPNYKNILYYNCSSYYA
                     ISIGLLSDKIIN"
     gene            502..1185
                     /locus_tag="EYO86_02500"
     CDS             502..1185
                     /locus_tag="EYO86_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41938.1"
                     /translation="MIKLLINLLIIFLLISCQLQKDNKIIKLIEDKKSFTKNTTISKK
                     NTTISKKNTTILEKNTIVSENKKVTTSLNILKYVVGDPYFIDGVEYIPSENYSYNKIG
                     LATYYGKELHNKKTVNNDLNKVTELLGRHKTLPIPSIVKITNLENGLSLIIKINDRHD
                     NNSSIIQVSRKTAQLLRFYKSKIARVKVEIIADPSKQIKIVTQSMNATNFNDTIKSAP
                     TEDVSISNL"
BASE COUNT          528 a          177 c          201 g          453 t
ORIGIN      
        1 tagtagatag tgatcaactt caaggtagtt ggggaggagc agtaggcatg acacaaatga
       61 ttcctacgac ttttcttgaa agtggttatg attgggatgg taatggtgtt gatatttgga
      121 atagttatga agatgctttt gccagtgctg ctaattattt gacatctttg aataaaaatc
      181 cgtggttaat tcacagtact tggggaaggg aagttcaacc tcctagtaat attgattcat
      241 tttatgatga tttaaaacaa gataacccaa aaggatgcgg agcggttaaa tcgagatcta
      301 tccccaaaag tttactagag tggagtgaat taggatttct tgatatcaat ggaaataaac
      361 ttccttctag acaaaattta gaagctcggc tcatagctcc tgatggatta aaaggaagaa
      421 tattcttagt ttatcctaac tataaaaata tcttatatta taactgcagt tcttattatg
      481 ctatttcaat tggactctta agtgataaaa ttattaatta atttattaat aatttttttg
      541 ttgatttctt gtcaattaca aaaagataat aaaattatta agcttattga agacaaaaaa
      601 agtttcacta aaaataccac catatcaaaa aaaaatacca ccatatcaaa aaaaaatacc
      661 accatattag aaaaaaatac cattgtttca gaaaataaaa aagttacaac aagcttaaat
      721 atacttaaat atgtagtggg cgatccctat tttattgacg gagttgaata tattccaagt
      781 gaaaattatt catacaataa aattgggtta gctacctatt atggcaaaga actccacaat
      841 aaaaaaactg ttaataacga tttaaataaa gttacagaat tgctaggtag gcataaaact
      901 ttacccattc ctagtattgt taaaattaca aatcttgaaa acggattatc attaattatt
      961 aaaattaatg atcggcatga taataattca tctattattc aagtatctcg taaaacagct
     1021 caattacttc gtttctacaa aagtaaaata gctagagtta aagttgaaat tatagctgat
     1081 cctagtaaac aaataaaaat tgtaacacag tcgatgaatg caacaaattt taatgatact
     1141 ataaaatcag ctcctacaga agatgtatct atatctaatc tttaggataa tttaatttca
     1201 gaaaatttga ataatgataa cttgaattct ccaattgcaa tggattttga agaagtgact
     1261 aatctagatt tgtatttaaa aatttatggt tttaatttat acattgaagt aaaaatctga
     1321 tttccgttct tgatattaaa tatatattta taacacaaa
//
LOCUS       DTVV01000210            2169 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1558295, whole genome shotgun sequence.
ACCESSION   DTVV01000210 DTVV01000000
VERSION     DTVV01000210.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1558295"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="EYO86_02505"
     CDS             complement(<1..994)
                     /locus_tag="EYO86_02505"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIC41939.1"
                     /translation="MNSEYCLEINNSNKTFPGVKALDNANFKLKKGSIHALVGENGAG
                     KSTLIKILTGVYKPDSGEIIINDEKINLNSPNDAILKGISVVHQERNLIGRFSVGENL
                     MLNNLPKNSLNLIDYNEVFKQSKKWLDILDLDINPETIVSDLTVAKMQLCEIAKALSM
                     ESKILLLDEPTSSISPQDVNNLFKLLKKIVSEEGVSVVFVSHKLEEVFQICDYVTVLR
                     DGKNACESEKIETLDRKQLVKLMIGREEQVVKSKKLFENNNNKILELENINTYLGHKS
                     INLSLYKGEILGLYGLVGAGRTELAKSIVGIEKIISGNLKLNSKSIKINSAKEAL"
     gene            complement(987..1967)
                     /locus_tag="EYO86_02510"
     CDS             complement(987..1967)
                     /locus_tag="EYO86_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_357077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC41940.1"
                     /translation="MNKIFSNLFKKDWFGLLLIVIFGALIIALLKPAFISSFNLYVLM
                     SSISLMLIVAMSQMIIIAIGQMNLSVGAIGGLVAISFAGLMEVYDMPIFIAASIGIVI
                     GVSAGFFNGYITAKTGISAFIITLATLYVFKGINLGITEAQPFYKIPDAIRVFGNGKI
                     IGPIPYLIIIPLIVTFVMWILMTKTIIGRYMLAYGNSIQSSELMGISSTQIVITAHVI
                     SGLLAGIAGMLVVCRLSLGTPNIGDAWLLPSFAAPVIGGAILAGGKVDVLATALAVVI
                     VAIISQALVIFKVDPFFVQIFLGFMILFAVLINRYREYQEENKKKIFVHE"
BASE COUNT          787 a          372 c          248 g          762 t
ORIGIN      
        1 ctaatgcctc ttttgcagaa ttaattttaa tagattttga atttaattta agatttcctg
       61 atattatttt ttcaatacca acaattgatt ttgcaagttc agttctacca gcgccaacta
      121 acccatataa acctaaaatt tctcctttat ataaagataa attaatagat ttatgtccaa
      181 gataagtatt aatattttct aattctaata ttttattatt attgttttca aatagttttt
      241 ttgattttac aacttgttct tcacgaccaa tcataagttt aactagttgt tttctatcta
      301 aagtttcaat tttctctgat tcacatgcat tttttccatc tcttaaaact gtaacataat
      361 cacatatttg aaatacttct tctaatttat gactaacgaa aacgacactt acaccttctt
      421 cagaaacaat tttttttaat aatttaaaaa gattgttaac atcttgagga gaaattgaag
      481 atgtaggttc atcaagtaaa agaatttttg attccatcga taatgctttt gcaatttcgc
      541 ataactgcat ttttgcaacg gtcaaatcgg aaacaattgt ttctgggttt atatctagat
      601 ctaaaatatc taaccatttt tttgattgtt taaaaacttc attataatca attaaattta
      661 atgaattttt aggtaaatta ttaagcatta aattttcacc aactgaaaat cttccgatta
      721 gatttctttc ttgatgaaca accgatattc cttttaaaat tgcatcattt ggagaattta
      781 aatttatttt ttcatcatta ataataattt caccactatc tggtttataa actcctgtta
      841 aaattttaat taatgttgat ttaccagcac cattttctcc aaccaaagca tgaatagaac
      901 ctttttttaa tttaaaattt gcattatcta atgctttaac tcctgggaaa gttttattgg
      961 aattattaat ttctaaacaa tattcactat tcatggacaa atattttctt tttattttcc
     1021 tcttgatatt cacgatatct attaattaag actgcaaata aaatcataaa tcccaaaaag
     1081 atttgaacaa aaaatggatc aactttaaat ataactagtg cctgagaaat aatagcaact
     1141 attacaactg ctaatgcggt tgccaaaaca tcaacttttc ctcctgctaa gattgctcca
     1201 ccaattacag gagccgcaaa agatggcaac aaccaagcgt caccaatgtt tggagtacca
     1261 agagataatc tacaaacaac aagcattcct gcaattcctg ctaacaagcc agaaataaca
     1321 tgtgctgtaa taactatttg agtagatgaa atacccatta attcagaaga ttgaatactg
     1381 ttgccgtaag ccaacatata tctaccaatt atagtttttg tcattagtat ccacataaca
     1441 aatgttacaa ttagtggaat tataattaaa taaggaattg gacctataat ttttccgtta
     1501 ccaaaaactc tgatagcatc tggaatttta taaaaaggct gcgcttcagt tattcctaaa
     1561 ttaatacctt taaaaacata taatgttgct aaagttatta taaaagctga tattcctgtt
     1621 ttagcagtta tgtatccgtt aaaaaaacca gcggatacac ctataacaat tcctatactt
     1681 gctgcaataa atataggcat atcataaacc tccattaatc cagcaaatga tatagcaaca
     1741 agtcctccaa tagctccaac agaaagattc atttgcccaa tagctattat aatcatctga
     1801 gacattgcaa cgataagcat tagacttatg ctggacatta aaacatataa attaaatgat
     1861 gaaataaacg ctggtttcaa taaggcgata attagagctc caaaaatgac aataagaaga
     1921 agtccaaacc aatctttttt aaataaatta gagaatattt tattcatgaa actacaatgt
     1981 ttttttctgt aaaatatgcc cttgctttat caatcaaaac cgtgcctagt aatattgctc
     2041 ctaaaaaaaa tcttacccag aatattccaa catcaagtaa taataaccca tttgtaatta
     2101 aactaactaa tactgctccc aatatagttc caaaaactgt aactttgcct ccagatagga
     2161 gtgtgccac
//
LOCUS       DTVV01000211            1414 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1566189, whole genome shotgun sequence.
ACCESSION   DTVV01000211 DTVV01000000
VERSION     DTVV01000211.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1566189"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="EYO86_02515"
     CDS             complement(<1..790)
                     /locus_tag="EYO86_02515"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC41941.1"
                     /translation="MIYYYFLAILAALCWSISSLISVDISRTIGGLVFNRLRLFFVSI
                     MLILYTTAIDTWKTIELDLLNIIILSGVIGVFFGDTLLFIALQKIGPRRNNILFALAA
                     PFTILLNIFFLKQNMTLIELIGCFTVFIGVVIAIAYGTNKENSHRWESIEGSLYLGII
                     FGILAALCQAIGLIIMKPILDLGVDPIASAAVRTSVSAILLSFTFMSNNKYLVSLNPI
                     TMKLVFKSIISGFLGMALGMSLLLVALQHADAGIVATLSSTSPVL"
     gene            980..>1414
                     /locus_tag="EYO86_02520"
     CDS             980..>1414
                     /locus_tag="EYO86_02520"
                     /inference="COORDINATES: protein motif:HMM:PF00730.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HIC41942.1"
                     /translation="MKPKYWNKGKEFLSKKDKVLKSIIDTFPNEHLLLNTNYYHSLIN
                     SIIGQQISVGAASSVRKKFFTLSRNITPNNVLKIKDTSMKKCGLSRQKTLYIKNISHF
                     FIYKKNFIKNITSFSEIEIINNLISIKGVGQWTAHMFLIFSIG"
BASE COUNT          563 a          208 c          204 g          439 t
ORIGIN      
        1 ccaaaacggg acttgtagag gaaagtgtag ctactatacc ggcatctgca tgctgaagag
       61 caaccagtaa taagctcatt cccaatgcca ttcctaaaaa accgcttatt attgatttga
      121 atactaattt cattgtgatt gggtttaaag aaacaagata tttattatta gacataaatg
      181 taaaactaag cagtatggcg gaaacagagg ttcttactgc tgcggaagca attggatcaa
      241 cacccaaatc aagaatgggt ttcattatta tcaatcctat tgcctggcac aaagcagcta
      301 aaataccaaa tataataccc agatataaag atccttcaat tgactcccat ctatgtgagt
      361 tttccttatt agtcccatat gcaatagcta taaccactcc aataaaaaca gtaaaacatc
      421 ctattaattc tattaaagtc atgttttgtt ttaaaaaaaa aatatttagc aaaattgtaa
      481 atggggcagc taaggcaaat agaatattat ttcttcttgg gccaattttt tgtaaagcaa
      541 tgaataataa ggtatctcca aaaaaaactc caataacgcc cgaaagaatg ataatattta
      601 ataaatcaag ttctatcgtt ttccatgtat ctattgctgt agtgtaaaga ataagcatta
      661 ttgaaacaaa aaacagtctt aaacgattaa aaactaagcc tcctatagta cgtgatatgt
      721 ccacagaaat taaagaagaa atggaccagc acaatgccgc taatatagct aaaaaataat
      781 aatatatcat taattgggca gcttaaaaaa tatcaatgat atttttaata ggataaagaa
      841 gtagttctcc taatggtgat ttttttatta agttggtaga tcgaaaaaag gcatatataa
      901 ttaatatcca taaataaatt ccaaacacaa aatttaaata agatacaatt tttttgaatt
      961 tattataata atacatacca tgaaacctaa gtattggaac aaaggtaagg agtttttatc
     1021 aaaaaaagat aaagttttga agagtattat agacaccttt ccaaatgaac atttattgtt
     1081 gaataccaac tattatcatt cgttaataaa ttcaatcata ggtcaacaga tatctgtggg
     1141 agcagccagt tcggttagga aaaagttctt cactttaagc agaaatataa ccccaaacaa
     1201 cgtattgaag atcaaggata cctcaatgaa aaaatgtgga ttgtccagac aaaaaactct
     1261 ttatattaag aatatttcac atttttttat ttataaaaaa aattttatta aaaatattac
     1321 ttcttttagt gaaattgaaa ttatcaataa tttaatttca ataaaaggag taggccaatg
     1381 gacggctcat atgtttttaa tatttagtat agga
//
LOCUS       DTVV01000212            2146 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1569782, whole genome shotgun sequence.
ACCESSION   DTVV01000212 DTVV01000000
VERSION     DTVV01000212.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1569782"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="EYO86_02525"
     CDS             complement(<1..927)
                     /locus_tag="EYO86_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41943.1"
                     /translation="MAIPFSLSLFRQKQSIINKLLISLSGYSFVLPPILVVYSVIGIY
                     GLKGILNETFNFYQLLDIKSLFGLKGILIAHVLLNAPFATRIFYQNLNTIPKSYVEVA
                     KSMNFGFWLNFFKLEWPIIKQNFFTVFLIIYILCFLSFAIVMALGGGPRTSTLEVAIY
                     QSVFFELNFNKAIVLSAIQITICLSLMLVGFFTLRGTNYFDIQTEDFIYPFSKRKLIV
                     YIDSGIIALFSIFLFSPIFYIFINFLKNLFNTNFYLNAHFIDAFTNSIILCLITGFFV
                     SFFGLVISIILVNCRNNFFYQQTLFILSSIILI"
     gene            complement(1126..2118)
                     /locus_tag="EYO86_02530"
     CDS             complement(1126..2118)
                     /locus_tag="EYO86_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HIC41944.1"
                     /translation="MKFLITFLVIFGLTTKSHSNNNELVIYTYDSFVSEWGPGPIIES
                     IFEEKYNKQIQFVSVDSAATLLTKVILEGSSTKADLVLGLDMNLINEAIDSNLFDSLT
                     IDDIDAKLSLPIKWEYKNFVPYNYGYFAFVYNNKNFKNPPQSMDELINHTDAKIVIQD
                     PRTSTPGLGLLTWIKSIYGKNAPREWSKLNKKIVSVTKGWSDSYYNIFLAGEADLVLS
                     YTTSPAAHIMFEKNNDYTAISFEEGNYLTVEFAGVLKSSKNKESAMEFMNFILSEDFQ
                     SIIPLTNIMYPVIKVKNLPEAFNSLNIPNKALQINPKTISNEKENWINEWLNAS"
BASE COUNT          824 a          294 c          291 g          737 t
ORIGIN      
        1 aattaatatt attgagctaa gaatgaataa agtttgctga taaaaaaaat tatttctgca
       61 gtttactaat attattgaaa taactagtcc aaaaaaactt acgaagaaac cagtaattaa
      121 acaaagtatt atggagttag taaaagcgtc aataaagtga gcatttaaat aaaaattagt
      181 attaaataag ttttttaaaa aatttataaa aatataaaaa attggtgaaa ataaaaaaat
      241 agaaaacagt gcgattattc cagaatcaat atagacaatt aattttcttt tgctaaatgg
      301 gtatataaag tcttctgttt gtatatcaaa ataattcgtt cctcttaaag taaaaaaacc
      361 aactaacatt agtgataaac atatagttat ttgaattgca cttaggacaa tagctttatt
      421 aaaattaagt tcaaagaaaa cagattgata gattgccact tccaaagttg atgttcttgg
      481 tccacctcct aacgccataa caatagcaaa acttaaaaaa cacaaaatat atattattaa
      541 gaaaacagtg aaaaaattct gcttaataat tggccattcc aatttaaaaa aatttagcca
      601 gaaaccaaaa ttcattgatt ttgcaacttc cacataactt ttcggtattg tatttagatt
      661 ttgataaaat attcttgttg caaaaggggc gtttagaaga acatgggcta ttaaaattcc
      721 ctttaaacca aacaaagact taatatccaa tagttgatag aaattaaatg tttcattaag
      781 aatccctttt aggccataga ttccaattac tgaatatacc accaatattg gaggtagaac
      841 aaatgaatat ccacttaaag atattaataa tttatttatt attgattgct tttgccgaaa
      901 caaactaaga gaaaatggaa ttgccaataa gcagctaaat aatgaagata aaaaagcttg
      961 aataaaagaa atatatataa ttcttatatt atatttgctt tgaaaaaatt taataaaata
     1021 ttctatttca aaatcaaatt tagtgaacaa gcctataata ggtaaaatta gtattaagga
     1081 cagtaaactt aatactagat aatatgattt ataaaacaat taaaattatg aggcattcaa
     1141 ccattcatta atccaatttt ctttttcatt gcttatagtt tttggattta tttgtaatgc
     1201 cttgttaggt atgttgagtg aattaaatgc ttcaggtaaa tttttaactt taattactgg
     1261 atacattata tttgttaaag gaatgataga ttgaaaatcc tctgaaagaa taaaattcat
     1321 aaattccatt gcactttctt tattcttaga ggattttaag acaccggcaa attctacagt
     1381 tagataattt ccttcctcaa agctaatagc agtataatca ttatttttct caaacattat
     1441 atgagcagct ggagaagttg tgtaactaag taccaaatca gcttcacccg ctaaaaatat
     1501 attgtaataa gaatctgacc aaccttttgt aactgataca atttttttat tcaatttaga
     1561 ccattctctg ggtgcatttt tgccatatat agatttaatc caagttaata accctaagcc
     1621 aggagtagat gttcttggat cctgaattac aatttttgca tctgtatggt taataagttc
     1681 atccattgat tggggcggat tcttaaaatt cttattatta taaacaaagg caaaataacc
     1741 ataattatat ggaacaaaat ttttatattc ccatttaata ggcaaagata attttgcatc
     1801 tatgtcatct atagtgagac tatcaaacag gtttgaatca atagcttcat ttattaaatt
     1861 catgtctagg ccaagaacta gatcagcctt tgttgatgag ccttccaaaa taacttttgt
     1921 aagcagtgtt gccgcgctat ctacagaaac gaattgaatt tgcttgttat atttttcttc
     1981 aaagatactt tcaattattg gtccaggacc ccattcggat acaaaagagt cataggtata
     2041 gataacaagt tcgttgttat ttgaatgtga tttggttgtt aaaccgaaaa tcactagaaa
     2101 tgttattaaa aatttcattt ttcctccgct ggcattatcc agatca
//
LOCUS       DTVV01000213            1018 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1574682, whole genome shotgun sequence.
ACCESSION   DTVV01000213 DTVV01000000
VERSION     DTVV01000213.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1574682"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            152..649
                     /locus_tag="EYO86_02535"
     CDS             152..649
                     /locus_tag="EYO86_02535"
                     /inference="COORDINATES: protein motif:HMM:PF14595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIC41945.1"
                     /translation="MKKFLLLFLLVLMNSSYSEELNHTIFISDNETLELPLPLPYKRI
                     EVTEEELKAFIANTKKKQAVIIFGANWCPDCRILAGTLKLPSVEKFMQRNYSILHIDL
                     GKYDINMALLEILGIPKQEGVPRVVIFDNKGKPINLDTNDRWRTARDSKQQEIFNYFQ
                     KYALN"
     gene            764..>1018
                     /locus_tag="EYO86_02540"
     CDS             764..>1018
                     /locus_tag="EYO86_02540"
                     /inference="COORDINATES: protein motif:HMM:PF00578.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIC41946.1"
                     /translation="MSYFYRLQKKKIAALFFVSLFSLSISAIEPGDKVENFRLLDQKG
                     GSHELFYYDDKKALVFLVQGNGCPFARNAAPRFQELRDIYS"
BASE COUNT          360 a          151 c          179 g          328 t
ORIGIN      
        1 acaaaaaaca gacaatattt ctctattgtc agagcggtaa cagatctgaa aaagctttag
       61 agattctcaa aaatttaggt tacataaaag taatcaattt gggcgggctt tcagatgcac
      121 aagagttatt aggggtagaa gttgttaaat catgaagaaa tttctacttc tatttttact
      181 ggttttaatg aactcaagtt actctgaaga actcaatcac acaatattca tttctgacaa
      241 tgaaacacta gaacttcctt tacctctacc ttataaaaga atagaagtta cagaggaaga
      301 actaaaggct ttcattgcaa atactaagaa aaaacaagct gtaattattt ttggagccaa
      361 ttggtgtcct gattgtagaa tacttgcagg taccttaaaa ctaccttcag tagagaaatt
      421 tatgcaaagg aattactcaa ttttgcatat agatcttggg aagtacgata ttaatatggc
      481 tttgcttgag attttaggca ttcctaagca agaaggtgtt cctcgggtag ttatatttga
      541 taataaaggg aagcccatta atcttgatac caatgatcga tggaggactg ctagagacag
      601 caagcaacag gaaattttta actatttcca gaaatatgct ttgaattaaa gaaaaccctt
      661 attctcaaag aaattaagtc tgtattaaaa catttgtatg atattcacaa aatgtaatta
      721 cagacctata attgaggact tgaattgtgg aaactattaa agaatgagtt acttctatag
      781 gttacaaaag aaaaaaattg ctgctctttt ttttgtttct ttgttttctt tatcaatatc
      841 cgcaatcgaa cctggcgata aagtagaaaa cttcagatta ttagaccaaa agggtggatc
      901 gcatgaactt ttttattatg atgataaaaa ggctttagtc tttttagttc aaggaaatgg
      961 ttgtcctttt gcaagaaacg cggctcctag attccaagaa ctcagagata tttattct
//
LOCUS       DTVV01000214            1224 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1593219, whole genome shotgun sequence.
ACCESSION   DTVV01000214 DTVV01000000
VERSION     DTVV01000214.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1593219"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1224)
                     /locus_tag="EYO86_02545"
     CDS             complement(<1..>1224)
                     /locus_tag="EYO86_02545"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIC41947.1"
                     /translation="WKKIIKLFKVLIKKVTNKDFSQDPVDQLWASIGAVLNSWMNQRA
                     KTYRSLNNIPESWGTAVNVQSMVFGNMGEDCCTGVAFTRNPSTGENNFYGEYLVNAQG
                     EDVVAGTRTPQNLTKKESTKQGTKDLSLEEYMPSIYRELEGIFDRLERHYLDMQDIEF
                     TVQRDKLWILQTRAGKRTTTAAVKIAIDMEKEGLIDKNEALSRINPLGLDQLLHPTLD
                     PQKEKKVLTKGLPASPGAASGKVVFDSEDAVLSSKKGESIILVRMETSPEDIHGMHAA
                     RGILTSRGGMTSHAAVVARGMGRPCVTGAGDLVIDHDKREFRVDDFVIKNNEVITIDG
                     GSGEVILGEIPTVMPGLSENFFQLMKWADEKRKLKIRANAETSRDVKTALDFGAEGIG
                     LCRTEHMFFDANRILA"
BASE COUNT          353 a          272 c          152 g          447 t
ORIGIN      
        1 agctaaaatt ctattcgcat caaaaaacat atgttctgtt ctgcataaac ctattccttc
       61 tgctccaaaa tctagagcag tttttacatc tctggaagtt tcagcatttg ccctaatttt
      121 aagttttctt ttttcatctg cccatttcat taattggaaa aaattctctg ataaacctgg
      181 cattacagtt ggtatttctc caagaataac ttctccgctt cctccatcaa ttgttataac
      241 ttcattattt tttatcacaa aatcatctac tctaaattcc ctcttatcat gatctataac
      301 taaatcacca gctcctgtaa cgcagggtct tcccatgcct ctagctacta cagcagcatg
      361 acttgtcatt cctcctctac ttgtcaaaat acctcgcgcc gcatgcattc catgaatgtc
      421 ttcaggacta gtttccattc gtaccaaaat gattgattca ccttttttag aacttaagac
      481 tgcatcctca gaatcaaata ctacttttcc tgaagctgca ccaggtgaag caggtaaacc
      541 ttttgttaac actttttttt ctttttgtgg atctaaagta ggatgcaaaa gttgatcaag
      601 ccctaaagga ttaattctag ataaagcctc atttttatct attaatccct ctttttccat
      661 atctattgct attttcactg cagcagtagt tgtccttttt cctgctctag tttgcaaaat
      721 ccataattta tctctttgaa cagtaaattc tatatcctgc atatctaaat aatgtctttc
      781 taatctatca aaaatacctt ccaattcgcg ataaatacta ggcatatatt cttcaagaga
      841 taaatctttt gtaccttgtt tcgtactctc ctttttagtt aaattttgtg gagttctagt
      901 tcctgcaacc acatcttccc cctgagcatt aacaagatat tcaccataga aattattttc
      961 tccagtcgag ggatttcgag taaaggctac cccagtacaa caatcttcgc ccatattacc
     1021 aaaaaccatt gattggacat taacagcagt tccccaagat tctggaatat tatttaagga
     1081 tctgtaagtt tttgccctct gattcatcca agaatttaaa actgccccta tagaagccca
     1141 tagttgatca actggatctt gtgaaaaatc tttattggta acttttttaa ttagtacttt
     1201 aaataatttt ataatttttt tcca
//
LOCUS       DTVV01000215            1604 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1599057, whole genome shotgun sequence.
ACCESSION   DTVV01000215 DTVV01000000
VERSION     DTVV01000215.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1599057"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            111..794
                     /locus_tag="EYO86_02550"
     CDS             111..794
                     /locus_tag="EYO86_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_770550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIC41948.1"
                     /translation="MQQFIYIASIWIIPVLISITMHEAAHGYAANLLGDDTAKKLGRV
                     TLNPLKHIDRFGTVILPLLLIVMKSPFVFGWAKPVPVKFYRLKNPLRDMIIVAIAGPV
                     TNVVLAFVAASILSTMHNFALLDNPWLVRTLANFFFINIILAVFNMIPIPPLDGGRVA
                     VGLLPQPLSYQLARLERYGFFIIITALFVLPLIGKKIGITLEPIHWFIQSVSSFLISI
                     IAFITGLQI"
     gene            851..>1604
                     /locus_tag="EYO86_02555"
     CDS             851..>1604
                     /locus_tag="EYO86_02555"
                     /inference="COORDINATES: protein motif:HMM:PF01075.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HIC41949.1"
                     /translation="MEKSILVFTNGEKLGDGIIKLPLLHEIKKRLPNYLLIWMTNKGK
                     TVYNSFLQSIAAQYIDEIIEQADLNPFFWQDISNSYNFQNKKYEYILDTQKAIFRTFA
                     LKRIKCEHFISAAGNGLFSSISINKQSKNIRQYYLDGLFNLLNVIKPDKIDDTFKINI
                     PEQLEKNLALIFKSQNKYIGIAPGAGEKNKIWPIEKFIEVGKYFEKKSYIIVLYLGPD
                     EIKIKHQLIAKFPKAVIPEDIIKDFSNIEIVMG"
BASE COUNT          517 a          251 c          258 g          578 t
ORIGIN      
        1 aaatcctcat gtcggtggtt caattccgcc cctgggcacc attaccctag tctttctgtc
       61 acgatctttt tcatttttca tttttgggtg aatggtgata tatttgggta atgcaacagt
      121 ttatttatat cgcttcaatt tggattatac ctgtcttaat ttccatcaca atgcatgagg
      181 ctgctcatgg ttatgctgca aatctcttag gagatgatac cgcaaaaaaa cttggacgtg
      241 taaccctaaa tccattaaaa cacattgata gattcggtac ggttattctt cctttgcttc
      301 tcatcgtgat gaaaagtcct ttcgtatttg gttgggcaaa accagtgccg gttaaatttt
      361 atcgtcttaa aaatcctctt cgtgatatga taattgttgc tattgctggc ccagttacaa
      421 atgtagtact tgcctttgtt gctgcttcaa tattatccac tatgcataat ttcgccttat
      481 tagataatcc ttggctggtt cgcactcttg caaacttttt ttttataaat attattctag
      541 cagtttttaa tatgatacct ataccgccat tggatggtgg aagggtagca gttggacttc
      601 ttccacaacc tctttcttat caacttgcca gattggaacg gtatgggttt tttattatta
      661 taactgcatt attcgttctt cctcttatag ggaaaaaaat aggaataacg ctcgagccaa
      721 ttcattggtt tatccaatct gtttcaagct ttttgataag tattattgca ttcattacag
      781 gattacaaat ttagttagtt gtaaaaaaat atttttttaa tatttttcta tattagtata
      841 gtgttatttt atggaaaaaa gtattcttgt ttttactaat ggagaaaagt taggggatgg
      901 cataataaag ctcccactgt tacatgaaat aaaaaaaaga ctgcctaact atcttttgat
      961 ttggatgacg aacaaaggca aaaccgttta taatagcttt ctccagtcaa ttgccgctca
     1021 gtatattgat gaaattattg aacaagctga tctcaatccc tttttttggc aagacatttc
     1081 taatagttac aattttcaaa acaaaaaata tgaatatatt cttgacacac aaaaagcaat
     1141 ttttagaaca tttgctttaa aaagaattaa atgcgagcat tttatttcgg ccgcaggtaa
     1201 tggtttgttt tcttctataa gtatcaacaa acaatcaaaa aatataaggc aatattatct
     1261 tgatggcctt tttaatttgt tgaatgtaat taaaccggat aagattgatg acacatttaa
     1321 aataaatata cctgagcaat tagaaaaaaa cctagctcta atatttaaaa gtcaaaataa
     1381 atatattggt attgcccctg gagcaggtga aaaaaataaa atttggccta ttgaaaaatt
     1441 tatcgaagtg ggaaaatatt ttgaaaaaaa gagttatata attgtcctgt atttaggtcc
     1501 agatgaaatt aaaattaaac atcaattaat tgctaaattt cctaaagcag taattcctga
     1561 ggatattatt aaagattttt ctaatattga aatagtcatg ggtt
//
LOCUS       DTVV01000216            1985 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1599883, whole genome shotgun sequence.
ACCESSION   DTVV01000216 DTVV01000000
VERSION     DTVV01000216.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1599883"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            133..1044
                     /locus_tag="EYO86_02560"
     CDS             133..1044
                     /locus_tag="EYO86_02560"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC41950.1"
                     /translation="MSHMKANVLMLIASLIWGTAFVSQTTGMGHIGPFTFGFARFFFA
                     MITVLPLAIIWEKNNVTKIIYNKNLLLLSLFTGFALFMGMGLQQYSLLKSQVSNASFL
                     STLYVPFVSLISRFIFKSRLTWIVWIAVVLCLYGSYLLSSNQAWDIQQSDSLLFIAAF
                     FFAVHIILVDIFMKQLYSPFSFGFIQYFIVFGCSLIIALLFENPILANIQLEWFEIIY
                     TGVFSAGIGYTLQIIAQSKAHPAPAAIILSMESVFGTIAAWFILSQTLDLNKIIGCVV
                     IFIGVVLVQLAPRYRHKNGRKNYMNNI"
     gene            1054..>1985
                     /locus_tag="EYO86_02565"
     CDS             1054..>1985
                     /locus_tag="EYO86_02565"
                     /inference="COORDINATES: protein motif:HMM:PF00294.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HIC41951.1"
                     /translation="MSNRLDVIGIGNAMVDALIPSTKEEVEKNQINRNSMNLIDEELK
                     NNLHKNYPIKKMAGGGSVGNSMFGITSLGGKGSFIGKIKEDKIGIFLQQDMIRVGLRF
                     PLEFTSPDISTGCCTIFIEEDGTRTMCTFLGAGTLIGPEDIHPEQIKNHQIAYLEGYL
                     WDNEKAKQAMQKMVNICEKDKQQIAFTLSDLFCVDRHRNSFEQLIKNHVDILFSNEEE
                     ICSMTQSSSIEDGIAYAKSLNIISIITLAEKGSLILNKDKVIEVSAKKVNKVVDTTGA
                     GDQYAAGFLYGLAKEKSLAECGRLGSIAAAEVIS"
BASE COUNT          646 a          323 c          366 g          650 t
ORIGIN      
        1 agaccgtata gcctactgga attttttaga atatccagaa gagattccgc tttatggtat
       61 cgtcattgaa tcatggtgga tcaatcaaga caaagctaat aaaattcaaa actaaaacaa
      121 aaagttaatt atatgagcca catgaaggcc aatgtattaa tgttgattgc ctcgcttatt
      181 tggggaactg cgtttgtcag tcaaacgact ggcatgggtc acattggtcc ctttactttt
      241 ggttttgcgc ggtttttctt tgccatgata actgttctac ctttagccat catatgggaa
      301 aaaaataatg taaccaaaat tatttataat aaaaatttat tactgctttc actctttact
      361 ggatttgctt tgttcatggg aatgggtttg caacaatact ctttgctgaa atcacaagtg
      421 tctaacgcat cttttctcag cactttgtat gtcccatttg ttagtttaat ctctcgtttt
      481 atttttaagt cacgattaac ctggatagta tggattgcag tagtgctttg tctgtatgga
      541 tcatatttat tatcttcaaa tcaagcatgg gatatacaac aatcggatag tttattgttt
      601 attgctgctt ttttctttgc tgttcatatt attctcgtcg atatatttat gaaacaactt
      661 tacagtcctt ttagttttgg gtttatacaa tattttattg tttttggttg ttcacttatc
      721 atagccttat tatttgaaaa ccctatccta gccaacatac agctagaatg gttcgagatt
      781 atttatactg gagtattttc cgcaggcata ggatacacac tacaaattat agctcaaagt
      841 aaggcgcatc ctgctcccgc tgctataata ttatccatgg aatcagtatt tggaacgatc
      901 gccgcatggt tcatcttatc ccaaactctt gatttaaata aaattatagg atgcgtagta
      961 atattcattg gtgtggtatt ggtacaatta gccccacgat ataggcataa aaatggtcga
     1021 aaaaactata tgaacaacat ataacacacc ataatgagta atcgtttaga tgtgataggg
     1081 atcggtaatg ccatggttga tgctttgatt ccttccacaa aagaagaggt tgaaaaaaat
     1141 caaattaacc gcaactctat gaacttgata gatgaagaat taaaaaataa tttacacaaa
     1201 aactatccca ttaaaaaaat ggcgggagga ggttcggttg gtaactctat gtttggaatt
     1261 acatcattgg gaggaaaagg aagttttatt ggaaaaatta aagaggataa aattggcatt
     1321 tttcttcaac aagatatgat ccgtgtaggc ttgcgctttc ctttagaatt tacatcaccc
     1381 gatatatcaa caggctgctg tacaattttt attgaagaag atggaacacg cacaatgtgt
     1441 acatttctcg gtgctggaac cttaataggt ccagaagata ttcaccctga gcaaataaaa
     1501 aatcatcaaa ttgcctacct agaaggatat ctctgggata atgaaaaagc aaaacaagct
     1561 atgcaaaaaa tggtaaatat ttgtgagaaa gataaacaac aaattgcttt taccctgtct
     1621 gatttatttt gtgttgatcg acatcgcaat tcttttgaac aattaattaa aaatcatgtt
     1681 gacattcttt ttagtaacga agaagaaata tgttcaatga cgcaatcatc gtccatagag
     1741 gacggcattg cgtacgctaa atcattaaat attatttcta ttattacttt agccgaaaag
     1801 ggatctttaa ttctcaataa agataaagtg attgaagtaa gtgcaaagaa agtcaataaa
     1861 gttgtcgata cgactggagc tggagatcaa tatgcagcag gatttctcta tggcctggct
     1921 aaagaaaaat cattggcaga gtgcgggaga cttggatcaa tagcagcagc ggaggttatt
     1981 tctca
//
LOCUS       DTVV01000217            1596 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1601070, whole genome shotgun sequence.
ACCESSION   DTVV01000217 DTVV01000000
VERSION     DTVV01000217.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1601070"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="EYO86_02570"
     CDS             <1..312
                     /locus_tag="EYO86_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIC41952.1"
                     /translation="KNLPDVCRNADILVAAAGRPEMIKGDWVKEGAIVIDVGINRIAT
                     QRDGKTKTKLVGDVDYNEAKKLASAITPVPGGVGPMTIACLLRNTAIAFKNSKNFFHQ
                     F"
     gene            complement(309..1496)
                     /locus_tag="EYO86_02575"
     CDS             complement(309..1496)
                     /locus_tag="EYO86_02575"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIC41953.1"
                     /translation="MPKKIVLAYSGGLDTSVILKWLQLKYKCPVVTFTADLGQGEELE
                     LVREKAESLGINEIFIEDLQEEFVREYVFPMFRANTLYEGNYLLGTAIARPLIAKRQI
                     EIAKKVGADAVAHGATGKGNDQVRFELGYYANNPNIEIIAPWRDWDFNSRNELIDFAE
                     KHQIPVPKDKRGETPFSTDANLLHTSSEGKILEDPWIEAPEFVYSRTKSLEEAAENSE
                     EIIIGFKNGDPIAINNKPLKPGELLSMLNSIAGKHGIGRIDIVENRFVGMKSRGVYET
                     PGGTILLHSHRAIESITLDKGEAHLKDELMPKYAEYIYNGFWFSSERIALQSLIDKTQ
                     NKVNGEVKLRLYKGNVIVIGRKSPNSLYNKSLVSFDEEGNYNQKDAEGFIKINSLRFK
                     NKN"
BASE COUNT          488 a          303 c          254 g          551 t
ORIGIN      
        1 aaaaatctgc cggatgtttg tagaaatgcc gatatattag tggccgctgc tggacgacct
       61 gaaatgatta aaggagattg ggtcaaggaa ggtgctattg taatcgatgt ggggattaac
      121 cgcattgcaa cacaaagaga tggtaaaaca aaaacaaaac ttgtgggaga tgtagattat
      181 aatgaagcaa aaaaattagc ttcggcgatt actccagttc caggtggtgt agggccaatg
      241 actatcgctt gcttattaag aaatacagca atagcgttca aaaattctaa aaattttttt
      301 catcaatttt aatttttgtt tttgaatcta agtgaattaa ttttaataaa accttcggca
      361 tctttttgat tataatttcc ttcttcatca aaggatacca agcttttatt atacaatgaa
      421 tttggagatt ttcttccaat gactattaca ttgcccttgt aaagacgtaa ttttacttct
      481 ccatttactt tgttttgtgt tttatcaatt aaagactgta aagctatcct ttctgaagaa
      541 aaccaaaaac cattataaat atattcagca tattttggca ttaactcatc ttttaaatgt
      601 gcttcacctt tatctaaagt aatactttca atcgctctat gagaatgtaa taatattgtt
      661 cctccaggtg tttcataaac acctcttgat ttcattccca caaacctatt ttcaacaata
      721 tcaatacgac caatgccgtg tttgccagca atagaattta acattgaaag caattcacca
      781 ggttttaatg gtttattatt tatcgcaata ggatcgccat ttttgaaacc aataataatc
      841 tcctctgaat tctccgctgc ttcttcaagt gattttgtcc gtgagtaaac gaattcaggt
      901 gcctcaatcc aagggtcttc tagtattttt ccttcagacg aggtatgcag taaattagca
      961 tccgtactaa aaggagtctc acccctttta tcttttggca caggtatttg atgtttctct
     1021 gcaaaatcta tgagttcatt acgtgaatta aaatcccaat ccctccaagg ggcaataatt
     1081 tctatgttag gattattggc ataataacca agttcaaaac gtacctgatc atttcctttt
     1141 cctgttgctc catgtgctac cgcatcagca ccaacttttt tagcaatttc tatttgacgt
     1201 tttgcgataa gcggtcgcgc gattgctgtt cctagtaaat aattaccctc atatagagta
     1261 ttggctcgaa acatcgggaa aacatattct ctaacaaatt cttcctgcaa atcttctatg
     1321 aatatttcat taatgcccaa tgattctgct ttttctcgaa caagttcaag ctcttctccc
     1381 tgacccaaat ctgcagtaaa tgtaacaacg gggcacttat atttaagttg taaccatttt
     1441 aaaattacac tcgtgtctaa tcccccagag tacgcaagaa caattttttt tggcattttt
     1501 aacaatcgtt aaaaagttga ttataagctg cagtaaatcc tttgagagta taaatatctg
     1561 tggttttagt gcctcgagat gattctcctt tgacta
//
LOCUS       DTVV01000218            3363 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1601422, whole genome shotgun sequence.
ACCESSION   DTVV01000218 DTVV01000000
VERSION     DTVV01000218.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1601422"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1..93
                     /locus_tag="EYO86_02580"
                     /pseudo
     CDS             1..93
                     /locus_tag="EYO86_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_428665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38..559)
                     /locus_tag="EYO86_02585"
     CDS             complement(38..559)
                     /locus_tag="EYO86_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41954.1"
                     /translation="MKTNDVDIINLGCRLNIYEGEVIKSLAHKNNLSNFTIINSCAVT
                     QEAEKKVKYEIRKSKKNFPEKKIIVTGCAAQINPQKYANIDEVDFVIGNKEKLQKQIW
                     SSLPNSNPVQVKDIFANNTIHNNIIEKFEGKSRAYIEVQQGCDHRCTFCVIPFGRGNR
                     KANQARRRTCWSL"
     gene            complement(565..1383)
                     /locus_tag="EYO86_02590"
     CDS             complement(565..1383)
                     /locus_tag="EYO86_02590"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:PRK00450.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIC41955.1"
                     /translation="MQKIDFIKMHGLGNDFVIIDNRIENIDISKNLIHQLSDRKSGAG
                     CDQLITINSSNESDIDASIEIFNPGGDRAEACGNGTRCVAKLLFDESQKKKLNILSDA
                     GILHAIKKDDSNISVNLGKLSTDWKKIPLSENIDTLNLPLTIDGFSNGVAVNIGNPHV
                     VFFGKNIDQVDLASIGPTIEKHNLFPNKTNVEIIEIINDQKINMRVWERGAGLTLACG
                     SGACASVYAGQLKKLLGNDVEVQLERGSLFITIDKGEAIMTGLAEISFRGNIEV"
     gene            1578..2945
                     /locus_tag="EYO86_02595"
     CDS             1578..2945
                     /locus_tag="EYO86_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIC41956.1"
                     /translation="MFGTLNERLEKIVRSVRGKAIISEADLDLTLREIRIALLEADVA
                     LVVVREFINNVKANIIGKEVLKSIKPDQMIIKLVQDELIQILGTNNQPLKIEKNGLTK
                     ILFCGLQGSGKTTTVAKLAYHIQKESKKKILLVSADIYRPAAQDQLKILSKQVNVEFF
                     DHQNSQSVEIITSESLLFAEKNLFDIILFDTAGRQVIDEVMMLELQKIHKQLQPQETI
                     LVADSLTGQDAANIAKSFNEAVTITGSILTRVDGDGRGGAALSIKSITGAPIKFIGTG
                     EKIEQLEAFYPDRIANRILGMGDIVSLVAKASENIDQAEMESLTEKMSKGTFDLDDFA
                     KQLKQMGKMGGVSGIISMLPGISKAQKLMAENKISDDLINHQIAIINSMTKKERANPD
                     SIKASRKIRISKGSGTKVQDVNKLLKQFLQSQKMMKKMKSMGKGGLPNDFMRKLQGKL
                     PPNIH"
     gene            2965..>3363
                     /gene="rpsP"
                     /locus_tag="EYO86_02600"
     CDS             2965..>3363
                     /gene="rpsP"
                     /locus_tag="EYO86_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIC41957.1"
                     /translation="MPVRIRLSRKGAKKHPFYRIVVADSRRSRDGKFIDQVGTYNPML
                     NKDNPERVKIDVEKAKEWISKGAQPSDRVKLFLSSMGVGEKPIITEKTKKHLPKKKTL
                     EKLAAAKKAKEVAKTEDAKPAAAEAPVEEVA"
BASE COUNT         1145 a          592 c          597 g         1029 t
ORIGIN      
        1 atccttgacg ccctggatgg gcgtatagcg cggctgctca aaggctccag caagttcggc
       61 gccgagcttg attcgctttc cgatttcctc tgccaaaagg aataacacaa aaagtacacc
      121 gatgatcaca accttgttgt acttctatat aagcacgcga ttttccttca aatttttcaa
      181 taatattgtt gtgaatagta ttattagcaa aaatatcctt aacttgaaca ggattactat
      241 ttggtagaga agaccagatc tgtttctgaa gcttttcttt attgcctatc acaaaatcca
      301 cttcatcaat attggcatat ttttgaggat taatttgggc ggcacatccc gtaacaataa
      361 tttttttctc cgggaaattt tttttagatt tacgaatttc gtattttacc tttttttcag
      421 cctcttgtgt aacagcacaa gagttaatta ttgtaaaatt agagagattg tttttatgag
      481 caagcgattt gattacttct ccctcataaa tatttaatcg acaaccaaga tttataatat
      541 caacatcatt tgttttcatg caaattaaac ctctatattg ccacgaaaac ttatttccgc
      601 taaaccagtc atgatagctt ctcctttatc gatggtgata aataacgatc cacgctccaa
      661 ttgcacttcg acatcgttgc ctaataattt ttttaactgt ccagcatata cagaagcgca
      721 tgcgccgctt ccacatgcca aggtgagacc tgcccctctt tcccatacac gcatattaat
      781 tttttgatca ttaattattt caataatctc aacatttgtt ttattgggaa atagattgtg
      841 cttttctatt gttggcccaa ttgaggccaa gtccacctga tcaatatttt ttccaaaaaa
      901 aacaacatga ggattgccaa tattaacagc aactccatta ctaaatccat caattgttaa
      961 gggaaggtta agggtgtcaa tattttcaga cagtggaatt tttttccagt ccgttgagag
     1021 tttgcctaaa ttaacactaa tattggaatc atctttttta atggcatgta aaatacctgc
     1081 atcagagagt atattaagtt tttttttctg agattcatca aataacagtt ttgctacaca
     1141 gcgtgtacca ttaccacatg cttctgctct atctccacct gggttaaaaa tttctatact
     1201 tgcatctata tctgactcgt tacttgaatt aattgtaatc aattgatcac atcctgctcc
     1261 acttttgcgg tcacttaact gatgaataag attttttgaa atatcgatat tttcaatacg
     1321 gttatcaata atgacaaaat cattgcccaa tccgtgcatt ttaatgaaat caattttttg
     1381 cataaaatct ctatatgact gtttttatac aaaattccac acaaaatcta tctaccttca
     1441 gaacttgact ttatagccct attttaatat gcgttcgctg aaattcctta attgtgattt
     1501 tactaactta tcaagttatt gataggtaca tcagcccacg agtatcgtgc cctggtgcaa
     1561 ttgtctattg gagaaacatg tttggaacat tgaatgaaag actagaaaaa attgttcgtt
     1621 ccgtaagggg aaaggcaatt atttcagaag ctgatcttga tttaacattg cgtgaaatac
     1681 gcatcgctct tttggaggca gatgtagctt tggttgttgt tagagagttc attaataatg
     1741 ttaaagccaa tattattggt aaagaagttc tcaaatcaat caagcccgat caaatgatca
     1801 ttaagctggt acaagatgaa ctaattcaaa ttctaggaac aaacaaccag cctctcaaga
     1861 ttgaaaaaaa cggcttaaca aaaattttat tttgtggatt gcaaggatca ggaaagacaa
     1921 ccacagttgc caagctcgca tatcacattc aaaaagaatc taagaaaaaa attttacttg
     1981 tatctgccga tatttatcgt cctgctgcac aagatcaatt gaaaattctg agtaagcaag
     2041 taaatgtgga attttttgat catcagaatt cacaatcggt agaaattatt acaagtgaat
     2101 ctctgctatt tgctgaaaaa aatttatttg atattatttt atttgataca gcgggacgcc
     2161 aagtcatcga tgaagtgatg atgttagaat tgcaaaaaat acacaaacaa ttgcaacccc
     2221 aagaaacaat tttagttgcc gattctttaa caggtcagga tgcagctaac attgccaaaa
     2281 gttttaatga ggctgtcact attacaggat ctattttaac tcgtgtagac ggtgatggaa
     2341 gagggggcgc tgcgttatca attaaatcca tcactggcgc gcccattaaa ttcattggaa
     2401 cgggtgaaaa aattgaacag ctagaagctt tttatccaga tcgtattgct aatcgtattt
     2461 tgggcatggg agacattgtt tctctggttg caaaagcatc tgagaatatt gatcaagcag
     2521 aaatggaaag cctaacagaa aaaatgtcaa aaggaacatt tgatttagac gattttgcga
     2581 aacaactaaa acaaatgggt aagatgggcg gggtgtcagg tattatatcc atgcttcccg
     2641 gtatttctaa agctcaaaaa ttgatggctg aaaataaaat ttcggatgat ctgattaatc
     2701 atcagattgc tattattaat tccatgacaa aaaaagagag ggcaaatccc gattcaatta
     2761 aagcttcacg aaaaattaga atttctaaag gttcaggaac aaaagttcag gatgtaaaca
     2821 aattattaaa acagtttctt caatcacaaa agatgatgaa aaaaatgaag tcaatgggca
     2881 agggcggttt acctaatgat tttatgcgaa aactgcaagg aaaactgcct ccaaatattc
     2941 attaacaaca ggaaaggaag tattatgccc gttagaatta gattatcaag aaaaggagct
     3001 aaaaaacacc cattttatcg tatcgttgtt gctgactcaa gacgatctag agatggaaaa
     3061 tttattgatc aagtaggaac gtacaatcct atgttaaaca aagacaatcc agaaagggtg
     3121 aagatagatg tagagaaggc aaaagagtgg atcagtaaag gagctcaacc aagcgatcgt
     3181 gttaaattat ttttaagttc gatgggtgtt ggggagaagc cgattatcac agaaaaaaca
     3241 aaaaaacatc ttccaaagaa aaaaacacta gaaaagttgg ctgcagcaaa aaaagcaaaa
     3301 gaagttgcaa aaacggaaga cgcaaaacct gcagcggcag aagcaccggt agaagaagtt
     3361 gca
//
LOCUS       DTVV01000219            1165 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1604902, whole genome shotgun sequence.
ACCESSION   DTVV01000219 DTVV01000000
VERSION     DTVV01000219.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1604902"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1165
                     /locus_tag="EYO86_02605"
     CDS             <1..>1165
                     /locus_tag="EYO86_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIC41958.1"
                     /translation="YHKSARIVGEVMGKYHPHGDSAIYESMVRMAQEFSLRLPLIDGQ
                     GNFGSMDGDPPAAMRYTEARLKKVSERLLDDLDKETVSFTENYDGSTFEPTVLPSKFP
                     NLLVNGASGIAVGMATNIPPHNLGEVIDGCMAYIDNKNITIDELTKFIKGPDFPTGGI
                     IKGRSGILSAFKTGRGSIVIESKVEIEERSKDKKKSIVITEIPYTINKSKLVEKIAEV
                     AKDKIVEDISDIRDESNRKGVRVVVEIKKGGNPDIILANLFKHTPLRTSFGVNALALK
                     DGTSPEVMDLKKMISIFISFREEVTRKRIFFELEKARQRVHNLTGLMIAVNNLDLVIK
                     LIRNSKNPNDAKISLLKKEWHAKDLTNFIKIVDDPRHKLKKAGKYDLSSEQANA"
BASE COUNT          462 a          140 c          217 g          346 t
ORIGIN      
        1 cttaccataa atcagcaaga attgttggtg aggtaatggg taaataccat cctcatggtg
       61 atagcgctat atacgaatct atggttagaa tggcacaaga gttttcttta agattgcctc
      121 taatagatgg tcagggtaat tttggttcaa tggatggaga cccaccagct gcaatgcgtt
      181 atacagaagc tagattaaaa aaagtatctg aaagattatt agatgacctg gataaagaaa
      241 cagttagttt tactgaaaat tatgacggct caacttttga acctactgta cttccatcta
      301 aatttccaaa tttattagtt aacggtgcaa gtggaattgc agttggaatg gcaacaaata
      361 ttccaccgca taatttagga gaagttatag acggatgtat ggcttatatt gataataaaa
      421 atatcacaat tgatgaatta actaaattta taaaaggccc agactttcct actggcggaa
      481 taataaaagg tagaagtggt atattgtcag catttaaaac tggtagagga tcaatagtca
      541 tagagtctaa agttgaaata gaagaaagat caaaagataa aaagaaatct atagttatta
      601 ctgaaattcc atacacaatt aataaatcta aattggtcga gaagattgct gaagtcgcga
      661 aagataaaat tgttgaggat atttccgata taagagatga atctaatcgt aaaggagtaa
      721 gagttgttgt tgaaataaaa aaaggtggaa atccagacat tattttagct aatttattta
      781 aacacacacc attaagaaca agttttggtg ttaatgcatt agctctaaaa gatggaacaa
      841 gtcctgaagt tatggattta aaaaaaatga tcagtatttt tatatccttt agagaagaag
      901 taacaagaaa aaggattttt tttgaattag aaaaagcaag acaaagagta cataatttaa
      961 cagggctgat gattgctgtt aataatttgg atttagtaat taaattgata agaaattcaa
     1021 aaaatcctaa tgatgcaaaa attagtcttt taaaaaaaga atggcatgca aaagatttaa
     1081 ctaactttat taaaattgtt gatgacccaa gacataaatt aaaaaaagct gggaaatatg
     1141 atttatcttc agaacaagct aatgc
//
LOCUS       DTVV01000220            1779 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1612133, whole genome shotgun sequence.
ACCESSION   DTVV01000220 DTVV01000000
VERSION     DTVV01000220.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1612133"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="EYO86_02610"
     CDS             <1..408
                     /locus_tag="EYO86_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIC41959.1"
                     /translation="KISEGTKVNEAKKFVDESGVDAIAISVGNIHLQTNKIAKIDIKK
                     IIDLQNVINIPLVLHGSSGIANAMRRKIAKTTNVAKFNIGTELRLIFGNALRANILQD
                     KDVFDRLKILKPTIKEIKKVAMKVILNIGPVNE"
     gene            401..1276
                     /locus_tag="EYO86_02615"
     CDS             401..1276
                     /locus_tag="EYO86_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine phosphorylase"
                     /protein_id="HIC41960.1"
                     /translation="MNNRLAFIGGSGLYDLNFLKNIKYLDISSSMGEPSEKIIEGSLG
                     DNKIFFLPRHGKEHRLSPSAINYRANIDSLKKCGVTDIISLSAVGSLKENLSPGTFVI
                     VDQFIDRTVNRIKSFFDDGIVAHVPMANPVSKELMLLSKSTLKNLQIPYNFGATYLAM
                     EGPQFSSLAESNLYRQWGCDVIGMTNMPEAKLAREAEIRYCSISMVTDYDCWHSGHDS
                     VDLKLVIKTLNENVDKSKLFIKMFSESYYQGIDFSNDKTSSILDTSIVTHKDSWNKDV
                     ENKLSFILKRYKEQN"
     gene            1277..>1779
                     /locus_tag="EYO86_02620"
     CDS             1277..>1779
                     /locus_tag="EYO86_02620"
                     /inference="COORDINATES: protein motif:HMM:PF01008.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIC41961.1"
                     /translation="MLVNGQHLTTIWYEENNDAIRIIDQRLLPHELKILTLHSLAEVL
                     FAIKEMLVRGAPLIGITAAYGMYIAARENSSIGFLRQSGENLKLTRPTAVNLSWAVDL
                     ILDKIINIEEINRPDYVLNLANQMRLADIQACKDIGENGFKLLETIYKKKNSTINILT
                     HCNAGWLA"
BASE COUNT          658 a          215 c          322 g          584 t
ORIGIN      
        1 aaaatatcag aaggaacaaa ggtaaatgaa gctaaaaaat ttgtcgatga aagtggagtt
       61 gacgctatcg ctatttctgt tggcaatata catttacaga caaataaaat tgcaaaaatt
      121 gatattaaaa aaattataga tttacaaaat gtaataaaca tacccttagt gcttcatgga
      181 agtagtggca ttgccaatgc aatgagaaga aaaatagcaa aaactacaaa tgtagctaaa
      241 tttaatattg gaactgaatt aagattgata tttggcaatg ctttaagggc aaatatttta
      301 caagataaag atgtttttga tagactaaaa atactaaaac ctacaattaa agaaattaaa
      361 aaggttgcta tgaaagttat tttaaatatt ggacctgtaa atgaataatc gtttagcttt
      421 tattggtggt agtggacttt atgatttaaa ttttttaaaa aatattaaat accttgatat
      481 ttcttcatca atgggcgaac catcagagaa aattattgaa ggtagtttgg gtgacaataa
      541 aatatttttt ttaccaaggc atggaaaaga acacagatta tcgccatctg caattaacta
      601 tcgagctaat attgatagtt taaaaaagtg tggagttaca gatatcattt cattatcagc
      661 tgtaggctca ttaaaagaaa atttgtctcc aggtactttt gttattgtcg atcagtttat
      721 agatagaacc gtcaatagaa taaaaagttt ttttgatgat ggtattgtag ctcatgtacc
      781 aatggcaaac ccagtatcta aagaattgat gttgttaagt aaatctactc taaaaaacct
      841 tcaaattcct tataattttg gtgccacata tttggcgatg gaaggacccc aattttcttc
      901 attggcagaa tcaaatttat accgtcagtg gggatgtgat gtaataggta tgacaaatat
      961 gccagaagct aaattagcca gagaagctga aatacgatac tgttctattt caatggttac
     1021 tgattatgat tgttggcatt ctggtcatga tagtgttgat ttaaagctag ttataaagac
     1081 tttaaatgag aacgtggata aatcaaaatt atttattaaa atgttttctg aatcttatta
     1141 tcaaggaatt gattttagca atgataagac atcaagtatt ttagatactt ctatcgtaac
     1201 acataaagat agttggaata aagatgttga aaataagtta tcttttattt tgaagagata
     1261 taaggaacag aattaagtgt tagttaacgg acaacattta acaacaattt ggtatgaaga
     1321 aaacaatgat gcaattagaa ttattgacca aagattgctt cctcacgaat taaaaatatt
     1381 aacacttcat tctttagcgg aagttttgtt tgcgataaaa gaaatgctag ttaggggagc
     1441 ccccttgata gggattaccg ctgcatatgg gatgtatatt gctgctagag aaaattctag
     1501 tattgggttt ttaagacagt ctggagaaaa tttaaaatta acaaggccta cggctgttaa
     1561 tctttcttgg gccgtagatt tgattttaga caaaataatt aatattgagg aaattaatcg
     1621 acctgactat gttcttaatt tagctaatca aatgagactt gctgatattc aagcttgtaa
     1681 agatattggt gagaatgggt ttaagctttt agagacaatt tataaaaaaa agaattcaac
     1741 gattaatatt cttacacatt gtaatgcagg atggctcgc
//
LOCUS       DTVV01000221            1701 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1612337, whole genome shotgun sequence.
ACCESSION   DTVV01000221 DTVV01000000
VERSION     DTVV01000221.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1612337"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(73..438)
                     /locus_tag="EYO86_02625"
     CDS             complement(73..438)
                     /locus_tag="EYO86_02625"
                     /inference="COORDINATES: protein motif:HMM:PF00009.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC41962.1"
                     /translation="MKTQINSSRCITIVGPNQSGKTSLMESILNVCKQIHNKGQVKTN
                     STLSDHLIEEHELGMSISMGVSNAQFMNEEYTFIDCPGSSEFINEFLQAARISDLSII
                     VMEPLKEKVLSLVPYFYYL"
     gene            complement(655..1443)
                     /locus_tag="EYO86_02630"
     CDS             complement(655..1443)
                     /locus_tag="EYO86_02630"
                     /inference="COORDINATES: protein motif:HMM:PF08003.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC41963.1"
                     /translation="MSLTDEGHYGPKQLNLLMTVWGEGFLSPGGTDEIDEVMKGIDAS
                     EKTVLDIGCGCGGAAIHLIKSHGAKSVLGIDIESLVIKRAEELAIKYDLSSLAHFRCV
                     KPGPLDILDESIDLVFSKEVFLHIPNKEDLIKDIYRVLKPGGRVAVSDWMRIDNNPPS
                     IQMQEYVAAEGLDMYMYSLKCYEQALKNAGFIDIAIKDRNAWYLGKAKKELAAIEGPL
                     NKQVIDAIGPEETAGAIDIWKKLIGVLELGEHRPGHFKAVKKKY"
BASE COUNT          555 a          323 c          269 g          554 t
ORIGIN      
        1 ctgaactgtg tccaatttta gctgaataaa ctttaggaaa ggagtactca gccttttcta
       61 atacgctact atttacaaat agtaaaaata aggaacaaga gataaaactt tttcttttaa
      121 aggctccata acaataatgc ttaaatcaga aattcttgct gcttgtaaaa attcgttaat
      181 aaattcacta gagccgggac aatcaataaa agtatattcc tcattcataa attgagcgtt
      241 tgagacgccc attgatatgc tcatccctaa ttcatgttct tcgattaagt gatcagacaa
      301 agtgctattc gttttaactt gacctttgtt atgaatttgt ttgcaaacat ttaaaatact
      361 ttccatcaaa gaagtcttcc cactttggtt tggcccgaca atagtaatgc atcttgaaga
      421 atttatttga gttttcatca gtttatccta ccaccaacca ctgggaatgt tgagggtaaa
      481 ttacagaatg ttgagggtaa atcacaatta agccaatctg taaagaactg gcatagcggc
      541 cacattgttt gcctcaaaat tgtttaggac aaatttccta aaacttcgct taaatattaa
      601 ggagaaaaat atatctatca agtgatccca acgacaatga ttaatgttga ggggttaata
      661 tttttttttg acagctttga aatgccctgg acgatgttct ccaagttcaa ggacgccaat
      721 taattttttc caaatatcga tggctccagc tgtttcttct ggaccgatag cgtcgatgac
      781 ttgtttattg agtggacctt caatagcagc aagttctttt ttagctttac caagatacca
      841 tgcgttacgg tctttaatag caatatcaat aaaaccggca ttctttaatg cttgctcata
      901 acacttaagt gaatacatat acatatcaag tccttcagct gcaacatact cctgcatttg
      961 tattgaagga gggttattat ctatccgcat ccaatcactt acagcaaccc taccaccagg
     1021 cttgagtact cgataaatat cttttattaa atcttcttta tttggaatat gaagaaatac
     1081 ctccttacta aacacaaggt caattgattc atctaaaata tcaaggggtc caggtttaac
     1141 acaacgaaaa tgtgcaaggc tagaaaggtc atactttatc gctagttctt cagcacgttt
     1201 aataactaat gattcaatat caattcccaa tactgatttt gcaccgtggc ttttgataag
     1261 atgtattgca gctccaccac atccacaacc gatatcaaga acagtttttt ctgatgcgtc
     1321 aatccctttc attacttcat ctatttcatc tgttccgcca ggcgaaagaa atccttcacc
     1381 ccatacagtc attaataaat ttagctgttt tggtccgtaa tggccttcat ctgttaagct
     1441 catattttat tcatcacgat tatcctacca ccaaccacga ggaatgttga ggggtattta
     1501 tttgtaaaat aataaagaat taagatatat gtttggcaaa ataatgaatt atgacaaaat
     1561 agacgcaaaa gaattaggcc aaagtcttaa aggacttggg attaatttgt tagtctctag
     1621 tgtaaaaaat acgagcacct ttttaagaac aatatttaat atagaacttt ttcaacaaac
     1681 tgatgatttc gccattgcaa a
//
LOCUS       DTVV01000222            4456 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1613941, whole genome shotgun sequence.
ACCESSION   DTVV01000222 DTVV01000000
VERSION     DTVV01000222.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1613941"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            41..700
                     /locus_tag="EYO86_02635"
     CDS             41..700
                     /locus_tag="EYO86_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41964.1"
                     /translation="MCSIFFVSVIPNLFFKIQNSKLPSSKILEGQKSSKIIISIIISL
                     ILFVNLGYSYVTFEVITTGNSVDTILENFKSPFSEKINPNKHDLDNIVTVLRNQPDIE
                     NSYVMANYIYFADIADAKWIGVQFQEGPEGDSIDNYITRKNWKSWEIYFSNISSEPND
                     RHNLNHPIPDYLIYNPKPFHLESLKVLADPTNSEIPKNFELLYKSPYSGITAYKINYN
                     D"
     gene            907..2823
                     /locus_tag="EYO86_02640"
     CDS             907..2823
                     /locus_tag="EYO86_02640"
                     /inference="COORDINATES: protein motif:HMM:PF13231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41965.1"
                     /translation="MQLKTLKSSLLKQPRYPFFITIVFFAVISYLTIFHNDFLKEGDF
                     FQYFLIGKEILDGNGTNVVALNANPGGPILYASLDKVFHDPLIVAKILGLFGGTGIVF
                     VSYYVMRNIFGSKVALIGQLFIAFNPKIILVSLAALNEILPVLFIFIAFYFITKKNIL
                     SNDVILCGVFLGLATMIRIQPILILIAFSIFLIFHSKKIQKNFCYVGLLVVFFLITVS
                     PVLAYNYNTHDVFIDSNPNYNYLNVSKYTTPEWIEKLKFNIITNQDYTILDYPELFLK
                     NYSYNLFYNNQDLFLNFGKMTSLSIIPTLPFVVLIPFLGGLFYLMKFRLDKINILVLS
                     STSLLSVFLLFLLGDPSIHFFIVIAIPTIALTLINIRNFQKNLLPLLISIFIFFTLIS
                     IIPLSRPEHLLPIWLIIPSLCAVFFVNTIPKILSKLSSSKKEVSISSQTKKIIIISVI
                     LVLLINVGHSYKSVELYLYENNYDGIVNEFSKFFQQSEPNIQKGQEIKKISDILSVQE
                     NIADSYVMAPMTGFLYYVDTNIILVTFQEGKKGDSFEQFYKRENWSEFDLLMSNVNSY
                     PADRYGKNNPIPNYLIYGNPPIMNWKHDIHSTQYDDLKILSDPNNPDIPSNFEFIYKS
                     DKLDLILYKIHHIP"
     gene            complement(2827..3876)
                     /locus_tag="EYO86_02645"
     CDS             complement(2827..3876)
                     /locus_tag="EYO86_02645"
                     /inference="COORDINATES: protein motif:HMM:PF00535.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIC41966.1"
                     /translation="MPLYQTSLMGITYTLLVLSFIAIIFLNLGWAITNLYFFIVEGVQ
                     TLLVGSTFVENIYFSTYLKWILLTDAIWIFSAVIFASTRQSYKTDSKLHYLNVNPINE
                     PKIFVIIPTYNEEEVVEKVINDYKNQKYVKEVLVVDNHSTDKTIEIAKLCSAKIIAKD
                     ENKGFAHSCIVGMRESLKTDANIIIFTECDGTFSGNDLEKMVPYLDNCDMVIGTRQVQ
                     VLSEKGNQNSTFYVWGNYFLAKLIQLKFFSLQHLGVVELTDVGCMHRCIRRESLEKII
                     DQFVEHDTDEPIISPKSGLFAILMTMIGIKNNLKIVEVPITFKKRIGISKTQSDKKIR
                     AVRYGLTFFWHIISK"
     gene            3912..>4456
                     /locus_tag="EYO86_02650"
     CDS             3912..>4456
                     /locus_tag="EYO86_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HIC41967.1"
                     /translation="MKCVILAGGYGTRISEESHMKPKPMIEIGGKPILWHIMKIYSVH
                     KINDFVICLGYKGDQIKEYFDRFDFTPWNIQLVDTGEDTMTGGRLKRIQDHIDDTFCA
                     TYGDGVSDVDINRLISFHKEKKSLATLTAIHPPERFGVLNLSGDYVTEFHEKHRGESS
                     WINGGFFVFEPKIFDYLQDDST"
BASE COUNT         1567 a          697 c          592 g         1600 t
ORIGIN      
        1 gtacaagcat tttctttatt tatggatatt tttggctcct ttgtgttcaa tttttttcgt
       61 ttcagtaata cctaatctat tttttaaaat ccaaaattca aaacttcctt cttctaaaat
      121 tcttgaaggt caaaaatcta gcaaaatcat catatcaata attatttctt taattttatt
      181 tgtgaatctt ggttattctt acgtaacatt tgaagtcatt acaactggaa attctgttga
      241 caccatcctt gaaaacttca agtctccgtt ttctgaaaaa attaatccta acaaacatga
      301 tcttgataat atagttactg tgttaagaaa ccaacccgat attgaaaata gctatgttat
      361 ggcaaattat atttactttg cagatattgc agatgcaaaa tggattggag tacaatttca
      421 ggaaggtcca gagggagatt caattgataa ttatattaca aggaaaaatt ggaaaagttg
      481 ggaaatctat ttttcaaaca ttagcagtga accaaatgat agacataact tgaatcaccc
      541 catcccagat tatctcatct ataatccaaa accatttcat ctagaatcat tgaaagtatt
      601 agctgatccc acaaattcag aaattcccaa aaattttgaa ttactctata aatctcctta
      661 ttctggaatt accgcttaca agatcaatta taatgattaa cttacaaatc atttattcct
      721 aaacaatatt aatattaaaa atacctcaaa aaaatgaaaa aaatactaat tacaatttat
      781 ggtgatacca cgaaaccaaa aaaattacca agttttaatc caagaatgcc tatcattgat
      841 ggactaatct ctgaatataa ttttatgaca actaatttga ttaaaatata aatccgagat
      901 aatattatgc agctaaaaac tctaaaatca tcattactaa aacaacccag atacccattt
      961 tttataacca tcgtattttt cgcagtaatt tcatatctga caatttttca caatgatttt
     1021 ttaaaagagg gggatttttt tcagtatttt ctaatcggta aagaaatttt agatggaaat
     1081 ggaacaaacg ttgtagctct taatgctaat cctggtgggc ctattcttta tgcctcgtta
     1141 gacaaggtat ttcatgatcc attaattgta gcaaagatac ttggtttgtt tggcggcact
     1201 ggaatagtat ttgtttctta ttatgtgatg agaaacatat ttggaagtaa agtagcgtta
     1261 ataggacagt tatttatcgc attcaaccca aaaattattc tagtatctct agcagcacta
     1321 aatgaaattt taccagttct tttcatcttc atcgcatttt atttcattac aaaaaaaaat
     1381 attctttcaa atgatgtgat attatgtgga gtctttcttg gacttgccac aatgattaga
     1441 atccaaccga ttttaattct cattgcattt tcaatatttc taatatttca cagtaaaaaa
     1501 atacaaaaaa atttttgtta tgttggatta ttagtggtat ttttcttaat tactgttagt
     1561 ccagtattgg cgtacaatta caatacacac gatgttttca ttgactcaaa tccaaattat
     1621 aactatctta atgtttcaaa gtatactact cctgaatgga ttgaaaaact gaaattcaat
     1681 attatcacta atcaagatta tacaattctt gattatccgg aattattttt aaaaaactat
     1741 agttataatt tattttataa caatcaagat ttatttttga attttggcaa aatgactagt
     1801 ttatccataa ttcctacgct tccatttgtt gttctaatcc catttttagg tggattattc
     1861 tacctgatga aattcagatt agataaaatt aacattcttg tattatctag cacttcctta
     1921 ctgtctgtat ttttgttatt cttgttaggc gatccaagca ttcacttctt tattgttata
     1981 gctatcccaa ccattgcact aacgttaatt aatattagaa attttcaaaa aaacttactt
     2041 cctcttttaa tatcaatttt tatatttttc actttgatat ctattatacc attaagtaga
     2101 cctgaacacc ttttaccaat ttggctaata attccatcat tgtgcgcagt attttttgtt
     2161 aataccatac caaaaatttt atcaaaatta tcttctagta aaaaagaagt ctctatttcc
     2221 tctcaaacaa aaaaaatcat tatcataagc gtaattctcg ttttattgat aaatgtagga
     2281 cattcataca aatctgttga attatatctt tacgagaaca attatgacgg aattgtaaat
     2341 gaattttcta agttttttca acaatctgaa ccaaacatac agaaaggaca agaaattaaa
     2401 aaaattagtg acatattatc agtgcaggaa aatattgctg atagttacgt gatggctcct
     2461 atgactggtt tcctatatta tgttgataca aatattatac tagtaacatt tcaagaaggc
     2521 aaaaaaggag actcctttga gcaattttat aaacgcgaaa attggtctga atttgatttg
     2581 ttaatgtcca atgtaaacag ctatcctgca gatagatatg gcaaaaataa tcctattcca
     2641 aattatctaa tttatggtaa ccccccaata atgaattgga aacatgatat tcactcaaca
     2701 caatatgacg atctaaaaat attatctgat ccaaataatc ctgacatccc atcgaatttc
     2761 gaatttatct ataaatcaga caaacttgat ctgattctat acaaaattca tcatatacca
     2821 taagcatcat ttagatataa tatgccaaaa aaatgtcaag ccgtacctta ctgccctaat
     2881 ttttttatct gattgtgtct ttgaaattcc aattctcttt ttaaaagtaa ttggaacttc
     2941 aactattttt aaattatttt taattccaat cattgtcatt aaaatagcaa ataatccact
     3001 tttaggacta attattggtt catcagtatc atgttcaaca aactgatcaa tgattttttc
     3061 taaagattct cttctaatac atctatgcat gcaacctaca tcagttagtt caacaacacc
     3121 taagtgttgt agtgagaaaa attttagttg aatcagcttt gctaaaaaat aattacccca
     3181 aacataaaat gtggagtttt gattcccttt ttcagataag acttgtactt gtctagttcc
     3241 aataaccata tcacaattat ctaaatatgg aaccattttt tccaagtcat taccactgaa
     3301 tgttccatca cattcggtaa aaattataat atttgcatct gtttttagcg attccctcat
     3361 accaactatg caggaatgtg caaatccttt gttttcatct tttgcaataa tttttgcact
     3421 acataattta gctatttcta tagtcttatc agtactgtga ttatcaacaa ctagaacttc
     3481 cttgacatac ttctgatttt tataatcatt gattactttt tcaacaactt cttcttcatt
     3541 atatgttgga attataacaa aaatttttgg ttcgttaatt ggattaacat ttaggtaatg
     3601 taatttagaa tcagttttat aggattgtct cgtggaagca aaaatcactg ctgaaaaaat
     3661 ccatatggca tctgttaaca gtatccattt taggtatgtt gaaaagtaga tgttttcaac
     3721 aaaagtagaa cctacaagta aagtctgtac accttctact atgaaaaaat acagatttgt
     3781 tatagcccag cccaaattta aaaagattat tgctataaat gataaaacaa gcagcgtgta
     3841 agttattccc atgagagaag tttggtagag tggcaatact tatgaattcg gatcaattcg
     3901 aattaactaa tatgaaatgt gtgatattag caggtggcta cggtactaga atttcagagg
     3961 aatcacacat gaaaccaaag ccaatgatcg aaataggtgg aaaaccaatt ttatggcata
     4021 ttatgaaaat ttattcagtt cataaaatta atgattttgt tatttgtttg ggttacaagg
     4081 gagatcaaat caaagaatat tttgacagat ttgattttac accatggaat attcaattag
     4141 ttgatacagg tgaagataca atgactggtg gtagactaaa aagaattcaa gatcatatag
     4201 atgatacatt ttgtgcaact tatggtgatg gtgtcagtga tgtcgatatc aatcgtttaa
     4261 tatcatttca taaagagaaa aaatctttag ctacattaac agctattcat cctccggaaa
     4321 gatttggggt actaaatcta tcaggagatt atgttacgga atttcatgaa aaacatagag
     4381 gtgaatcttc ttggataaat ggaggatttt ttgtctttga accaaaaatt tttgattatt
     4441 tacaggatga ttctac
//
LOCUS       DTVV01000223            1747 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1621651, whole genome shotgun sequence.
ACCESSION   DTVV01000223 DTVV01000000
VERSION     DTVV01000223.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1621651"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="EYO86_02655"
     CDS             complement(<1..1254)
                     /locus_tag="EYO86_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIC41968.1"
                     /translation="MFKSIDYKRFFISLFLISTFIGVTLVNLDTASNLFNSTQSFIAN
                     NFGWLIILSANGFLIFCIWIAISKFGEIRLGGTDAKPEFNFINWIAMLFSAGLGIGVI
                     FYSVAEPVLHLSSSALFEEGVSFNERATLSMNLTFLHWGFHAWAIYGVVGLCFAYFAF
                     NLGRPFRVSSFFLDTRLESAWNRVIGVIVDVFAILATVFGIATSLGLGASQISAGLKH
                     LNIANSYWIPIETLNPEASLGKFIVIIFITILGLISVVLGLNAGIKRLSQLNMILCGC
                     FLITIFLFGPTGYILDGFVENVGSYIQNFISLSTNVNAYSDSDWQNAWTLFYYCWWFA
                     WSPFVGLFIARISYGRTIKEFILGVVFLPSLIVFIWLSVFGNAAIYQEFTTAGSLANA
                     VNENISVSLFIFLEQYPGSTLLMGLS"
     gene            1505..>1747
                     /locus_tag="EYO86_02660"
     CDS             1505..>1747
                     /locus_tag="EYO86_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41969.1"
                     /translation="MTRKQHKALRRSKAITKKQNILRIKNRAKSAAVVAAVSAVIATP
                     NISAQDTSAQATIEEIVVTSRKKTENLQDVPVSVSTL"
BASE COUNT          661 a          318 c          273 g          495 t
ORIGIN      
        1 agaaagaccc attaaaagag ttgaaccagg atactgttct aaaaaaatga aaagtgatac
       61 tgaaatattt tcattgacgg cattcgctag tgatccagct gtcgtaaatt cttggtatat
      121 tgctgcattt ccaaacacac tcaaccatat gaaaacaatc aatgagggta aaaaaacaac
      181 cccaaggatg aattccttga tggttctacc ataagaaatt cttgctataa aaagacctac
      241 aaaaggtgac catgcaaacc accaacagta atagaataat gtccatgcat tttgccagtc
      301 agagtcactg taggcattta cattagttga cagactaata aaattttgaa tgtaagaccc
      361 aacattttct acaaatccat ccagaatata tccagtaggt ccaaataaaa atatagttat
      421 caaaaaacaa ccacacaaaa tcatatttaa ttgcgagagt ctttttatgc cggcattcag
      481 tcccaaaact acacttataa gacctaatat agtaataaag ataataacta taaactttcc
      541 aagggaagct tcaggattta atgtttctat tggtatccaa taactatttg caatattaag
      601 atgtttaagc cctgcgctta tctgacttgc tcccaagccc aatgatgtag ctatgccaaa
      661 gactgttgcg agaatagcaa acacatcaac tatcactcct atcactcggt tccaggcact
      721 ttccagtcga gtatctaaaa agaatgagct gactctgaaa ggtcttccta aattaaatgc
      781 aaaatatgca aagcataatc ctacgacacc gtatatagcc caagcatgaa acccccaatg
      841 taaaaatgtt agattcattg ataaagtggc ccgctcattg aaagagacac cttcttcaaa
      901 gagtgccgat gagctgagat gcagaacagg ttctgcgact gagtaaaata ttactccaat
      961 acctaatcca gcactaaata gcatagcgat ccagtttata aaattaaatt ctggtttcgc
     1021 atcagttcca cccaatctaa tttctccaaa cttactaata gctatccata tacaaaaaat
     1081 tagaaaacca tttgcactta aaataattag ccatccgaag ttgttagcta taaatgattg
     1141 tgtgctatta aaaagattgc tggcagtatc aaggttaact agggtaaccc ctataaaagt
     1201 tgagataaga aataaagaaa tgaagaatct cttataatct atacttttaa acataattag
     1261 ttaaaagaat tcttttgtaa gacattttta gagagaagta gcgtttcaaa ggacaattta
     1321 tattatcttt aatgactgtc aagtaattat tatttttagt attacgtaat acgaaataat
     1381 acgaaaataa tttttttaat cctaaaagca tgaattttaa tgagttctgt aaatttaaga
     1441 taaaatatac taaactttgc ctaataacta ttggatattt actcatctaa tcgggagata
     1501 aaggatgacg agaaagcaac ataaggcctt aagaagatca aaggctatca caaagaaaca
     1561 aaatatactt aggattaaaa acagagcgaa atctgctgct gttgtcgcag ctgtaagtgc
     1621 cgttattgct actcctaata taagtgctca agatacaagt gctcaagcaa ccatcgaaga
     1681 aattgttgta acatcaagaa agaaaacgga gaacctgcaa gatgttcctg tgtcagtttc
     1741 tacacta
//
LOCUS       DTVV01000224            1199 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1628185, whole genome shotgun sequence.
ACCESSION   DTVV01000224 DTVV01000000
VERSION     DTVV01000224.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1628185"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="EYO86_02665"
     CDS             <1..652
                     /locus_tag="EYO86_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIC41970.1"
                     /translation="YSLIHDDLPSMDNDELRRGKLTVHKKFNEFTAILAGNSLLTLAF
                     EILSGNNLQLSQNYKNELIKTLSMCAGHSGLAGGQYSDLTFENKSILKKNIINMQKKK
                     TGELFGFCCESIAIIKGVNSIKRKKLKEIGINIGLLFQIVDDLIDYKGDSMIVGKPTR
                     SDEKKGKATLVNLLGYEETINFAKNLKKKINQQIKKYGTKANDLLDSVEFILKRKF"
     gene            653..>1199
                     /locus_tag="EYO86_02670"
     CDS             653..>1199
                     /locus_tag="EYO86_02670"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594649.1"
                     /note="catalyzes the formation of 1-deoxy-D-xylulose
                     5-phosphate from pyruvate and D-glyceraldehyde
                     3-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIC41971.1"
                     /translation="MSKTSLLDKINYPADLRKLRKEELKQLAKELREELIDVVSTTGG
                     HLGAGLGVVELTIALHYVFDTPKDKLVWDVGHQAYPHKIITGRRDNIRTLRKGGGLSG
                     FTKRSESEYDTFGAAHSSTSISSTLGIAVAKSLSNNSNHAIAVIGDGAMSAGMAYEAM
                     NNAGAMKSKLIVILNDNDMSIA"
BASE COUNT          478 a          159 c          222 g          340 t
ORIGIN      
        1 atattcgtta atacacgatg atctaccctc aatggataat gatgaattaa gaagaggaaa
       61 gttaacggtg cacaaaaaat ttaacgagtt taccgcaata ttagcaggaa attctttact
      121 gactctagca ttcgaaattt tatctggaaa caatttgcaa ctatctcaaa attataaaaa
      181 tgaactaata aaaactttat caatgtgcgc aggccattcc ggtttggctg gtggacaata
      241 ctctgatttg acttttgaaa acaaaagtat cttaaaaaag aatattatta atatgcaaaa
      301 gaaaaaaacg ggtgaactat tcggtttttg ttgtgaaagt attgctatta ttaaaggggt
      361 aaactctata aaaagaaaaa aactaaaaga aattggtata aatattggtt tattatttca
      421 aattgttgat gatttgatcg attataaagg agacagtatg atagttggaa aaccaactag
      481 aagtgatgaa aaaaaaggca aagcgacatt ggttaattta ttaggatatg aagaaacaat
      541 aaattttgcc aaaaatctta aaaaaaaaat aaatcaacaa ataaaaaagt atggtactaa
      601 agctaacgat ttattagatt ctgttgaatt tatattaaaa agaaaatttt aaatgtcaaa
      661 aactagttta ttagataaaa tcaattatcc cgcagatcta agaaaattaa gaaaagaaga
      721 acttaaacaa ttagcaaaag agctgagaga agaattaatc gatgttgttt caacaaccgg
      781 tggacacttg ggggctggct taggtgtagt ggaattaact atcgcactgc actacgtatt
      841 tgatacacca aaagacaagc tggtttggga tgttgggcac caagcttatc ctcataaaat
      901 aataactggt agaagagata atattagaac cttaagaaaa ggtggcggtc tatcaggttt
      961 tacaaaaaga tcagaaagtg agtatgatac tttcggagca gcgcacagtt caacttctat
     1021 ttcctcaaca ttaggaatag ctgttgcaaa aagcctatca aataattcta accacgctat
     1081 tgcggtaata ggtgatggag ctatgagcgc tggcatggct tacgaggcaa tgaataatgc
     1141 tggcgcaatg aaatcaaaat taattgtaat acttaatgat aacgatatgt caatagcac
//
LOCUS       DTVV01000225            1180 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1637440, whole genome shotgun sequence.
ACCESSION   DTVV01000225 DTVV01000000
VERSION     DTVV01000225.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1637440"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(227..406)
                     /locus_tag="EYO86_02675"
     CDS             complement(227..406)
                     /locus_tag="EYO86_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41972.1"
                     /translation="MNRIKRVHLIYPVGNKISTPDAIVRNLKLSLENVYEVITYNYDE
                     YRTINPGKADVLIGH"
BASE COUNT          339 a          243 c          185 g          413 t
ORIGIN      
        1 tccaaatgaa ctatttttgg ttgccaatga tgaaacggtg aatctttcaa gcgtttcttc
       61 caagcattac ctataattgc caaaaaacga tcacattttg caataatctt gttcccaaat
      121 gcattgtgtt agcccgtggg gtcgtgacaa aatggcacaa gagctataac acgtttccat
      181 cctttttttt ggcgctcatc ctaaaaactg tgaatggatt tggatgctaa tgtccaatta
      241 aaacatctgc tttgccagga tttatagtac gatattcgtc gtaattatat gttataacct
      301 catagacatt ttctaaagat agttttagat tccggactat cgcatcgggt gtactgattt
      361 tatttccaac gggatatatt aagtgtaccc gttttattct gttcatctaa tcatctttct
      421 caaaagtttt tttagaaacg agaatattgt aatttgatag cttatcttct ctattacttg
      481 gtctgctctt gtatctctct ctgtcatatc cggagttgtt agaggaaagt taatctcact
      541 ggttgctaat tcgtgattgt ggtcttttgt cagcgtatga gccccttgag tccatccgat
      601 attttgtaca agattttttg atgaaacaat aacctgctgt tttttttgcc aaagcaaata
      661 ttgtaattga atgtcccagg tatcaatttt tccgcttcct acttcctcaa acatatcttg
      721 ccaatacctt ttcaaaaata tatcggtatg ggaaaatata tcctttttcc aagtattttg
      781 tttttctatt aacaaattta tattggagtc atatccttca ttagaccgtc tccaaccagc
      841 ccagccccaa actcgcaagt atcttgaaaa aaagaatgac gcgcttggtt tgtattctaa
      901 ttcgtttgag taaaatccac aaattactcc aactgcttta tcatttcgaa aatactcaag
      961 catttcttgg caaaaccaaa aaaagctttg actaggcaga caatcatctt ccaaaataat
     1021 cccttcaggc tcctgctcaa aaaaccagta aatggcttga cctacagcca gccggcagcc
     1081 aatattttga tctcgaaaaa gagttttgac ttcacagtcc cagtcaacat ttgtcgcaat
     1141 ttttcgtgaa tgttcacatt tctcatcctc atttggataa
//
LOCUS       DTVV01000226            1310 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1640832, whole genome shotgun sequence.
ACCESSION   DTVV01000226 DTVV01000000
VERSION     DTVV01000226.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1640832"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="EYO86_02680"
     CDS             complement(<1..488)
                     /locus_tag="EYO86_02680"
                     /inference="COORDINATES: protein motif:HMM:PF02737.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41973.1"
                     /translation="MKIGIIGLGFVGLSFGSVLASKGHSVIGVDVDKEKLKKIKDGVI
                     PFYEPGLQSILKKALKKDLKVSSSISDVEKCSLIFVTVGTPQKRSGEIDLTMVKNVID
                     KIGKLLSKTQHKPIIIIKSTVIPGTTRDVILSILQRVSGKKVGKDFGLVTNPEFLRET
                     MAV"
     gene            complement(518..>1310)
                     /locus_tag="EYO86_02685"
     CDS             complement(518..>1310)
                     /locus_tag="EYO86_02685"
                     /inference="COORDINATES: protein motif:HMM:PF00483.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41974.1"
                     /translation="PITKELPKEMMPIFSRIYNNNLIVIPLLQFIFEQLYAVGIRDYC
                     FVVGREKRSIEDHFTPDETYLNGLSRKHNVLMTNFYKRLEKSHLIWTNQNKPLGFGDA
                     VKRAEKYIGNDNFIVHGGDVSIIGYSKHPVLRLIKTAKDDPSIAAVLLCKKIKDPKRY
                     GVPQVKKTSSSVYLVEKVEEKPNIPKSDLGILPIYFFKPKIFKSLKNIKPDKNNELQL
                     TDAIQNLIELGEKVVAIPIKSNEIEIDIGTAETYKHALETSYKIA"
BASE COUNT          401 a          233 c          182 g          494 t
ORIGIN      
        1 accgccatag tttcccttaa aaattctgga ttggtcacaa gaccgaaatc ttttcctacc
       61 ttcttgccag atacacgttg taaaattgat agtatcacat cgcgcgttgt tccaggaatt
      121 acagtactct ttattattat aattggttta tgctgagttt tagaaagtaa ttttccaatt
      181 ttatctatta cattttttac cattgttaaa tcaatctctc cacttctttt ctgaggagtt
      241 ccaacagtaa caaagatcaa actacatttt tctacatctg aaatacttga cgatacttta
      301 agatctttct ttagagcttt tttaagaatg gattgtaacc ctggttcata aaatggtatc
      361 acaccatcct ttattttttt taatttttcc ttatcaacat ccactccaat aactgaatgt
      421 cccttagaag ccaacaccga gccaaaagat aaaccaacga atcctaaacc gattattcca
      481 attttcatat cccaacaaac ttactagatg ttaaaaatta tgctatttta tatgaagttt
      541 ctagagcgtg tttgtaggtc tcagctgttc caatatcaat ttcaatttca tttgatttga
      601 taggaatagc aaccactttt tcgcctagtt caattagatt ttgaatggca tctgttaatt
      661 gtagttcatt attcttatct ggttttatgt ttttaagcga cttgaatatt tttggtttga
      721 aaaagtaaat tggtaatatt cctaaatctg atttaggaat attgggtttt tcttcaactt
      781 tttcaactag ataaactgat gatgaagtct ttttcacttg tggaactccg tatcttttag
      841 gatcctttat ttttttacaa agaagtacag ctgctataga aggatcatct tttgcagttt
      901 taattaatct taaaactgga tgtttggaat agcctataat ggaaacgtct ccgccatgaa
      961 caataaaatt gtcatttcca atatattttt cagcacgttt tacggcatct ccaaatccca
     1021 atggcttgtt ttggtttgtc catataagat gcgatttttc taatcttttg taaaaattcg
     1081 tcatcaaaac attatgcttt ctcgataatc cgttgagata agtttcatct ggcgtaaaat
     1141 gatcttcaat tgatcttttt tctctaccca ccacaaaaca ataatctctt attccgacag
     1201 catacagctg ttcaaaaata aattgaagca agggaataac aatcagatta ttattatata
     1261 ttctagaaaa aataggcatc atttctttgg gaagttcctt agttatagga
//
LOCUS       DTVV01000227            2095 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1641214, whole genome shotgun sequence.
ACCESSION   DTVV01000227 DTVV01000000
VERSION     DTVV01000227.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1641214"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="EYO86_02690"
     CDS             complement(<1..320)
                     /locus_tag="EYO86_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41975.1"
                     /translation="MGLFSRNKDNVRGVVSSETQDWNKDLKRNTASKNVVSQQGFDFI
                     ENIGTKGILSTLETKMGLSLNVENISSDQVRILKNCNTENTAAELIKILKRTNKTKFK
                     QIIL"
     gene            556..759
                     /locus_tag="EYO86_02695"
     CDS             556..759
                     /locus_tag="EYO86_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41976.1"
                     /translation="MGVYELPLTSHCYKIIGRGDLDYVKLRRILSLLLLRLTINKGVF
                     LEKRQSPKNNCYRGNDKQWLWMD"
     gene            complement(886..1239)
                     /locus_tag="EYO86_02700"
     CDS             complement(886..1239)
                     /locus_tag="EYO86_02700"
                     /inference="COORDINATES: protein motif:HMM:PF08238.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HIC41977.1"
                     /translation="MSQMNQHLLAESELNLETIDLQQRAYTGDAEAQYALANIFQKGI
                     HVQKNTKHAFYWYQSAAHQGNLLAQYNVWFAYLTGEGIQANTQQANKWFARASLKNSR
                     SAQSIVAQLLDTTIH"
     gene            1479..>2095
                     /locus_tag="EYO86_02705"
     CDS             1479..>2095
                     /locus_tag="EYO86_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIC41978.1"
                     /translation="MANKTKDYLAKVRKKTGLSDYKIAQKYDINQSNLSKYKSGKTAL
                     SETHAWQFASILGVNPAIVVANTKLEHAKLTGNKLKAIFWQEQLENLSNGSEPIKIDI
                     AQINPIVGDLKNNAQTIINLAYDADESGTHLLVFPELALIGYPAEDLLLREGFIDQIE
                     STIEHMRTQLPEGISVLFGAPARIDHHLYNSAYLIRQGRVSTYHK"
BASE COUNT          697 a          365 c          345 g          688 t
ORIGIN      
        1 tctaaaatta tttgcttaaa ttttgtttta ttggttcttt ttaaaatttt aattaactcg
       61 gcagcagtgt tctcagtatt gcaatttttt aaaattctaa cttggtcact gcttatattt
      121 tcaacattta aacttagtcc catcttagtc tctaaagtgc ttaaaatccc tttagtacca
      181 atgttttcta tgaaatcaaa accttgctgt gaaacaacat ttttgctagc agtattacgc
      241 ttaagatcct tgttccaatc ttgagtttcg ctgctaacca cgccccttac attatctttg
      301 ttcctactaa acaaacccat aaccaataaa taaatttaaa acaatatgta aagtttaaca
      361 aaatcatttg ttttattgaa agattattta atgaattgtg ttaaatatta aacaaaatag
      421 ctgcttcttt agcctggata ctagtagtga tagatgttta cgttaatctg taaattaacg
      481 cctatttgcc acgttataga cccttcaaag ggtaaattac tcataacaaa tactttgcag
      541 tattaaaaaa aataaatggg cgtgtatgaa ttacccctaa caagtcattg ttacaaaatt
      601 attggaagag gtgatcttga ctacgtcaag ttaagaagaa tcctaagtct gttactatta
      661 agactgacga ttaataaagg tgtatttttg gaaaaaagac aaagccccaa gaataactgt
      721 taccgaggta atgataagca atggctttgg atggattaat ggagaataag agctgtatat
      781 tgatgctaat gcggagctag caaccaataa tgcaaacaat gttaatgttg atttaatcaa
      841 ttgcaaagta tgcatgactt aactccttat cttttaaatt ataaattaat gtatggttgt
      901 gtctaacaac tgagccacta tagattgtgc agagcgagaa ttctttaaag atgccctagc
      961 aaaccatttg ttagcctgtt gcgtattcgc ctgaatcccc tcacctgtta aataagcaaa
     1021 ccaaacgttg tattgtgcta gcaaattacc ctgatgtgca gcgctctgat accaataaaa
     1081 agcatgtttt gtgttctttt gaacatggat acctttttga aaaatattgg ccaatgcata
     1141 ctgcgcttct gcgtcacccg tataagccct ttgttgtaga tcaatggtct caagatttaa
     1201 ctcagattct gctaataaat gttggttcat ttgactcata agaatttcac ctgtttattg
     1261 ttgatttatg tgaaatatac cattactttt ttagcttgta aagtgctttt ttataaaaaa
     1321 attaattatt aatattttag tgttaaatca acaacttata ttgtaattat ggtactttta
     1381 ccactactgg tattttttaa ctaaaatgaa tgatttacac tagtggtata aaataactat
     1441 aatagttaaa aaatacaagg aattttaagt aaaaaatcat ggcaaacaaa actaaagact
     1501 atctggctaa agtacgcaaa aagactgggc tctcggacta taagatagct caaaaatatg
     1561 acattaatca gtccaatcta agcaaatata aatcaggaaa aacagccttg tctgaaaccc
     1621 atgcttggca atttgccagt attttgggtg ttaatcctgc catagtggta gctaacacca
     1681 aacttgagca tgcaaaactc acgggcaata aattgaaggc catattttgg caggagcagc
     1741 tagaaaacct ttcaaatggc tcagagccta tcaaaattga tattgcacaa attaacccta
     1801 ttgttggtga tttgaagaat aacgcgcaga cgatcatcaa tctggcgtat gacgcggatg
     1861 aatctggcac tcatttactg gtttttccag aattagcgct gattggttac ccagcagaag
     1921 acttattgct gcgtgaaggc tttattgatc agatcgaatc taccattgag cacatgcgta
     1981 cccagctacc tgaaggcatc agtgttttat ttggtgcccc tgcacgcatt gatcatcatt
     2041 tatacaacag tgcttactta atacgtcaag gccgtgtaag cacttatcac aaaca
//
LOCUS       DTVV01000228            1632 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1654390, whole genome shotgun sequence.
ACCESSION   DTVV01000228 DTVV01000000
VERSION     DTVV01000228.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1654390"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="EYO86_02710"
     CDS             <1..576
                     /locus_tag="EYO86_02710"
                     /inference="COORDINATES: protein motif:HMM:PF01791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HIC41979.1"
                     /translation="SGIPLIVKVNGKANLTKGDVISRQVCSVKKAISIGAKGIGYTIY
                     AGSSYESEMFQEFGRIQEEAHDHGLPAISWVYPRGGKIPDLGGDSTKETVAYAARIGL
                     ELGADAVKIKYSGNSEDFKWAVQSAGKAHVFMSGGPKTNTDEDFLKLVTGVMDAGATG
                     VAVGRNVWQHQDPLNITAKIKEIIFEGKRVD"
     gene            603..1622
                     /locus_tag="EYO86_02715"
     CDS             603..1622
                     /locus_tag="EYO86_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIC41980.1"
                     /translation="MKIAILTGGGDAPGLNPVIRSVVKVAKENKIEMIGIKKGWAGLI
                     ELDHFPLKWEDVKNLHSEGGTFLLSSRTNPIKMENGIKRVSDNLNQIADGLIAMGGED
                     TLGVANELYKNGVNVVGVPKTIDNDLFSTDYTIGFDTAINITVSAIDRIRTTGRSHER
                     FMVVEVMGRHAGWLAVHSGLASGADLILYPEEKYDVQEICEKIKKLKHEGQKSGIIVI
                     AEGAKEKEGSHTTQDKELDAFGHVKLGGVGEKLSKVIEKNTGIETRHIVLGHLVRGGT
                     PSAFDRVYGARLGYGAIKLVLEKKWGQMLTLRGTEIRSMPLEEGVKELKTVTNDYRDL
                     IRQLT"
BASE COUNT          661 a          202 c          378 g          391 t
ORIGIN      
        1 tcaggaattc ctctcatagt taaagtaaat ggaaaagcca atctaacgaa gggcgatgtt
       61 atatcaagac aagtgtgttc ggtgaaaaaa gcaattagta ttggagcaaa aggaattgga
      121 tacactatct atgcaggaag ttcgtatgaa tcagaaatgt ttcaagaatt tggcagaata
      181 caagaagaag cacatgatca tgggctccca gcaatatcat gggtataccc aagaggagga
      241 aaaattcctg atcttggagg ggattcaaca aaagaaacgg tggcatatgc ggcacgaatt
      301 ggtttagaac taggtgcaga tgctgtaaag ataaaatatt cagggaattc agaggatttt
      361 aaatgggcag tgcaatctgc gggcaaggca catgtgttca tgtctggcgg tccaaaaaca
      421 aatacagatg aagatttttt gaaactagta accggagtaa tggatgcagg agcaacagga
      481 gtagctgtgg gaagaaatgt atggcagcat caagatccac taaatattac ggcaaaaata
      541 aaagagataa tatttgaagg taaaagagta gattaaatca acttattgaa tttagggaaa
      601 aaatgaaaat agcaatacta actgggggcg gtgatgcgcc tggattaaat cctgtaatac
      661 gctcagtagt aaaggtagct aaagaaaata aaattgaaat gataggcata aaaaaaggat
      721 gggcaggatt aattgaatta gatcattttc cattaaagtg ggaagatgtt aagaacttac
      781 attcagaggg cggtacattt ttactatcga gtagaacaaa tcccataaaa atggaaaatg
      841 gaataaagag agtttcagat aatttgaatc aaatagcaga tggtcttatt gcaatgggag
      901 gagaagatac tctaggtgta gcaaatgaat tatataaaaa cggagtaaat gttgtgggtg
      961 taccaaaaac aattgataat gatctttttt caacagatta tacaatagga ttcgataccg
     1021 caataaatat cacagtaagt gcaattgatc gaataaggac tacaggcaga tcacatgaaa
     1081 gattcatggt agtagaggta atgggcagac atgcaggatg gttagcagta cattcaggat
     1141 tagcatctgg agcagattta atattatatc cagaagaaaa atacgatgtt caagaaatat
     1201 gtgaaaaaat caaaaaatta aagcatgaag gtcaaaaaag cggcattatt gtaattgcag
     1261 aaggagcaaa agaaaaagaa ggatctcaca ctacacaaga taaagaatta gatgcatttg
     1321 gtcatgtaaa attaggagga gtgggagaaa aattatcaaa agtaattgaa aaaaatacag
     1381 gaatagaaac aaggcatata gttttaggtc atttagtaag aggaggcaca ccttcagcat
     1441 ttgatagggt atatggtgcc agactgggtt atggagcaat aaaattagta ttagaaaaaa
     1501 aatggggtca aatgctcact ttaaggggaa cagaaataag atcaatgccg ctagaagaag
     1561 gagtaaagga gctaaaaaca gtgacaaatg attacagaga tttaatcaga caattaacat
     1621 aaacaaataa ca
//
LOCUS       DTVV01000229            1038 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1657958, whole genome shotgun sequence.
ACCESSION   DTVV01000229 DTVV01000000
VERSION     DTVV01000229.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1657958"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(495..857)
                     /locus_tag="EYO86_02720"
     CDS             complement(495..857)
                     /locus_tag="EYO86_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIC41981.1"
                     /translation="MEKQKFIAHAKEGKILARKCINCGNLHLVTVYFCEKCGKKAFDD
                     VILDGQGSVETYTIMTVPPTGFEKYAPYAWVVMSIDNVDLRISGFLPNIAKPEDLPIG
                     TKVKISGFDNRGIILEKP"
     gene            complement(862..>1038)
                     /locus_tag="EYO86_02725"
     CDS             complement(862..>1038)
                     /locus_tag="EYO86_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HIC41982.1"
                     /translation="LKSKGHPIGATGVGQVVEVYDQLTGNAGERTVKNAKIGLTHNFG
                     ATGASCAVHIFQRV"
BASE COUNT          319 a          205 c          140 g          374 t
ORIGIN      
        1 ccatcacaga aaagatcatt cttacacgtt tcgaggtaga ttgaataatc gtgcctcctg
       61 atttttcatc taagtcaaga tcaagttgat catctaagtc aaaattgtca tccatgtcca
      121 tatttaattc atgatcgagc tctccttcaa gctctaattt tttactgctc cccaacatcg
      181 ttagtaaata aacggagtaa ttaagactta tcgcatgatc aagttttgaa ttttttcaaa
      241 atctcagaat gtgaaaaata gttatttttt ttctgcatct aaagaaaaat cagatattga
      301 tagtttttgt ccgcaagaag gacatttatt attataatgc tttagtgtat ctttgattga
      361 ctttagcatc ttcattgtag aaattttttc tccacatttt ccacacttta catcaacagg
      421 catcatcaaa taaatattat taaatcatat taaatttttg ttaaaaaaaa aattacagta
      481 acgctaaaat tgatttacgg tttttccaaa attattccac ggttatcaaa accagatatt
      541 ttgacttttg taccaattgg aagatcttct ggctttgcta tatttggtaa aaaccctgat
      601 atccgcaaat caacgttatc gatactcatc actacccaag catatggtgc atatttttca
      661 aaacctgtgg gcggaactgt cataattgtg tatgtctcta cgcttccttg accatctaat
      721 ataacatcat caaatgcttt cttcccgcat ttttcacaaa aataaacagt aaccagatgt
      781 agatttccac agtttatgca tttacgagct aggatttttc cctctttagc atgagctatg
      841 aatttttgct tttccataga tttacactct ttgaaaaata tgaacggcgc aacttgctcc
      901 agtagcacca aagttatgtg ttaatccaat ctttgcattt tttacagttc tttctcccgc
      961 atttccagtt aactggtcat aaacttctac aacttgaccc acaccggttg ctccaattgg
     1021 atgtcccttt gatttaag
//
LOCUS       DTVV01000230            1476 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1661981, whole genome shotgun sequence.
ACCESSION   DTVV01000230 DTVV01000000
VERSION     DTVV01000230.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1661981"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="EYO86_02730"
     CDS             complement(<1..259)
                     /locus_tag="EYO86_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HIC41983.1"
                     /translation="MNNFIESIIKRDPAARNKINVILAYPGVKAVFFHFIAHKLWELK
                     IYLIARIISQFSRFLTGIEIHPAAKIGKNLFIDHGMGVVIGE"
     gene            complement(405..683)
                     /locus_tag="EYO86_02735"
     CDS             complement(405..683)
                     /locus_tag="EYO86_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41984.1"
                     /translation="MRIIYLIIIIFIFSLCAQAKEKNNILNKKEIVILGLTLVNPAIS
                     IGTIVSNTTTFQKIISMANIVYTNRKGHSIAETAISQSTNKNCLIIKT"
     gene            complement(740..1462)
                     /locus_tag="EYO86_02740"
     CDS             complement(740..1462)
                     /locus_tag="EYO86_02740"
                     /inference="COORDINATES: protein motif:HMM:PF01066.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HIC41985.1"
                     /translation="MNINKINTDKIAAWAVHGFTASGAVLGFLAIISILNNDLVGAFL
                     WLGLALLIDGIDGTLARKIGVLDKTPNIDGSTLDNVIDYLNYVIIPALMIYWFQMVPN
                     GWEIILPAGMFAVSLYTFANMNMKTNDYYFSGFPAVWNIVVLYFYILNTHPIINVIII
                     LFLFIFTFIPIKFVHPLRVKKIRNLTIFCTVLWSATTLKLVTTNSDINLFAEQKIVLL
                     IWIVCSIYFATICGTRSFKDLH"
BASE COUNT          556 a          214 c          181 g          525 t
ORIGIN      
        1 tctcacctat aactacaccc attccatgat caataaaaag gttttttcct attttggcgg
       61 ctgggtggat ttcaatacct gtcaaaaagc gactaaattg agaaattatc ctagcaataa
      121 gataaatttt taattcccat aatttatgag ctataaaatg aaaaaataca gcttttacac
      181 ccggatatgc taaaattaca ttaattttat ttcttgcggc tggatctcgc ttgataatag
      241 attcaataaa attattcata tatacaatct ttaaatagtt attattaagg ttaattcaat
      301 tatttttata ttattaattc tctttgtttt cttatagaca tcttttctaa tgataaaata
      361 gtgaatactt gatttatatc aaatacaaaa atatttctta gttcttatgt ttttataatc
      421 aagcaatttt tatttgtact ttgtgagatg gctgtctcag caatagaatg tccttttcta
      481 tttgtataaa caatgtttgc catagatata attttttgaa aagtagttgt gttggatact
      541 atagttccga ttgatattgc tggatttacc aaagttaatc caagaattac aatttccttt
      601 ttatttaata tattgttttt ttctttagcc tgagcacata atgaaaatat aaatataatt
      661 attattaaat atataattct catattttga tactacttca attattgata ttttcaaatt
      721 tataaggtca aaaaagacat taatgcaaat ctttgaaaga ccttgtacca catatagttg
      781 caaaataaat gctacataca atccatatta acaaaacaat tttttgttca gcaaataaat
      841 taatatctga atttgtagtt actaatttta aagttgtagc tgaccaaaga acggtacaaa
      901 atattgttaa gttacgaatt ttttttaccc taagaggatg aacaaacttt attggaatga
      961 aagtaaaaat aaacaaaaat aaaataatga ttacattaat aattggatgg gtatttagaa
     1021 tgtaaaaata caaaacaacg atattccaaa ctgctggaaa gccggaaaaa tagtaatcat
     1081 tggttttcat attcatattt gcaaaggtgt ataaagagac agcaaacata cctgcaggca
     1141 aaatgatttc ccatccatta ggtaccattt gaaaccaata aatcattaat gctggaataa
     1201 ttacataatt tagataatcg attacattat caagtgtaga accatcaata ttgggcgttt
     1261 tatccaagac cccaattttt cttgctaaag ttccatctat accatcaatt aacaaagcta
     1321 aaccaagcca caaaaaagca ccaactaagt cattgtttaa aatagaaata attgctagaa
     1381 atcctaaaac cgcacctgag gcagtaaaac catgcactgc ccaagcagca attttatctg
     1441 tattaatttt attaatgttc atatttaact ataaaa
//
LOCUS       DTVV01000231            2638 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1662811, whole genome shotgun sequence.
ACCESSION   DTVV01000231 DTVV01000000
VERSION     DTVV01000231.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1662811"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="EYO86_02745"
     CDS             complement(<1..671)
                     /locus_tag="EYO86_02745"
                     /inference="COORDINATES: protein motif:HMM:PF13231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41986.1"
                     /translation="MSTLRINSSEAYSDRKYYILITIGFFLVTTYVSFFHHDFWTIFD
                     QDGLIYLSGGQQIIAGVGENVSFPNAGPAGPVLFALLESVLKDGLIAIKIVSILSGTG
                     IVFFSYMVFKNIFSSKIALVGQLFIAFNPWIGILSISSVNDLFPIFMSIFSLYFITKK
                     DIKLTDIIFSGAILGIGFMFRAQPAIFLLAIITFLILLRKKPKFKISAVALLIVFFVL
                     CCSPM"
     gene            complement(673..1899)
                     /locus_tag="EYO86_02750"
     CDS             complement(673..1899)
                     /locus_tag="EYO86_02750"
                     /inference="COORDINATES: protein motif:HMM:PF02655.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIC41987.1"
                     /translation="MGSLKKTLLILSGGIEAIEGIKIAKKMGLRTIVCDGNENAPGKS
                     YGDEFLVANIYDPYEIKNVISEYSKKESIDGVITIASDAVRSVSTVAKQHNLPGISLK
                     TSILATNKLKMKKAFQQYSLPIPNFVKINCRNELHRQIQHFDKAVLKPVDNRGSRGVI
                     IINKNSDINKAFDYSIKFSPTRKLILEEWLSGSQLSTESLVISGKTHLCGIADRNYSN
                     QKDTFPYIVEDGGETPSRYYPKIKNKLEKILDTTAKSLGIENGVLKGDLVLKNNNIYL
                     IEAATRLSGGFFSTITIPLVYRISLIEKAILLALGSKIVPPPKFLKHYCYQANRFLFP
                     KPGLIKKVIKPNQEKLPKYVKYIEVNAKVGERVDSIKHHPMRKGSVLVIGKTRSEAIK
                     RAEKIRALIKIETVQN"
     gene            complement(1887..2618)
                     /locus_tag="EYO86_02755"
     CDS             complement(1887..2618)
                     /locus_tag="EYO86_02755"
                     /inference="COORDINATES: protein motif:HMM:PRK12826.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIC41988.1"
                     /translation="MRLKDKVCIITGSSSGIGKEIAKGFAKEGAHVVITYLTNKKSAH
                     KLAKQINAKLVLQLDIKKRSSIRRVFKKIIKEYGHIDVLVNNAGINLPADFDKQTDAE
                     WNEVIDVNLTGVFRCCQEVLPYIVDYGRIINIGSLSGEYGGPRTPSYTCAKMGLMGLT
                     HNLARFLGPRNICVNTLSPGVIESKMTKKTISPKVRKAVLSLALFKRFGQYSEIVGGA
                     IFLASDESSYMTAQTLSINGGAWVL"
BASE COUNT          838 a          453 c          373 g          974 t
ORIGIN      
        1 aacataggtg aacaacataa aacaaaaaat acaattaaca gtgcaacagc agaaatttta
       61 aacttcggct tttttctaag taatattaaa aaagtaataa ttgctaaaag gaaaattgca
      121 ggctgagctc taaacataaa tccaatgccc aaaattgcgc ctgaaaaaat gatgtcggtt
      181 aattttatgt ctttttttgt aataaaatat aaggaaaaaa ttgacataaa gattggaaaa
      241 agatcattaa ctgaagaaat tgaaaggatg cctatccaag gattaaacgc aataaacagt
      301 tgtcctacta gcgctatctt tgagctaaag atgtttttaa aaaccatata ggaaaaaaac
      361 acaataccgg taccactaag aatagagact atctttatcg caatcaaacc atcttttaaa
      421 acagattcaa gtaacgcaaa aagaactggt cccgctggtc ctgcatttgg aaatgaaacg
      481 ttttctccaa ctcccgcaat aatttgttgc cctcctgaaa ggtaaattag accatcttga
      541 tcaaatattg tccagaaatc atgatggaaa aatgaaacat aggttgtaac taggaaaaat
      601 cctattgtga ttaagatata gtattttcta tcactatatg cttctgagga attaattctt
      661 aatgtactca ttttaatttt gaacagtttc tattttaatc agagccctaa ttttttcagc
      721 tcgtttaatt gcttcacttc ttgttttgcc aataacgaga acacttccct tacgcatagg
      781 atgatgtttt atagaatcaa ctctctctcc aacttttgca tttacttcaa tatacttaac
      841 atattttggt agtttttctt gatttggttt tattactttt tttattaaac ctggttttgg
      901 aaaaagaaat ctatttgcct gataacaata atgtttaagg aatttaggcg gtggcactat
      961 tttagaacct aaagccaata aaatcgcttt ttcaattaag cttattctgt atacaagtgg
     1021 aatagttatt gtagaaaaaa atccgccact taatctcgtt gcagcttcta ttaggtaaat
     1081 attattattt tttagaacta ggtctccttt taatacacca ttttctattc cgagagattt
     1141 tgcggtagta tccaaaattt tctctaattt atttttgatt tttggatagt acctgcttgg
     1201 agtttctcct ccatcttcta caatgtaggg aaatgtgtct ttttgattcg aataatttct
     1261 gtctgcgatc ccgcatagat gagtttttcc tgaaattact aatgattccg tgcttaattg
     1321 agaaccagat aaccattctt ctagaattaa tttcctagta ggtgagaact ttattgaata
     1381 atcaaacgct ttattgatgt cagaattttt attaattatt attacgcctc tagagcctct
     1441 attatctaca ggttttagta ctgctttatc aaaatgttgg atttgtctat gtaattcatt
     1501 tctacagtta atcttaacaa aatttggtat gggtaatgaa tattgttgaa acgctttttt
     1561 cattttcagt ttatttgtag ctaaaattga ggttttaaga ctaatacctg gtaaattatg
     1621 ttgtttagca actgtagata ccgatctaac agcatcactt gcaattgtta taactccgtc
     1681 aatagattct ttttttgaat attcagaaat cacatttttg atttcataag gatcatatat
     1741 gttagcaaca agaaattcgt ctccataaga ttttcctggt gcattttcat ttccatcaca
     1801 aacaatagtt cggagaccca ttttttttgc aatcttaatt ccttcaattg cttcaatacc
     1861 tcctgatagt atcaaaaggg ttttttttaa agaacccaag ctccgccgtt aatacttaat
     1921 gtttgagcag tcatatacga agattcgtct gaagctaaaa atattgcccc tccaactatt
     1981 tcactatatt gtccaaatct tttaaataaa gccaatgata aaactgcttt ccgtacttta
     2041 ggcgaaatag ttttttttgt cattttactc tctattacac caggtgatag tgtgtttaca
     2101 caaatgtttc taggaccaag aaatcttgct aaattatggg ttaatcccat taaacccatt
     2161 tttgcacatg tataggatgg agttcgtggt cctccatatt cacctgacaa agaacctatg
     2221 ttaattatcc taccgtaatc gacaatatat ggtaaaactt cttgacaaca acgaaatact
     2281 ccggtaagat ttacgtctat aacttcattc cattctgcat cagtttgctt atcaaagtct
     2341 gcaggtaaat ttattcctgc attattaact aaaacatcaa tatgtccata ttcttttata
     2401 atttttttga atactcttcg tatacttgat ctctttttga tatccaactg taaaactagt
     2461 ttagcattga tttgttttgc taatttatgt gcactttttt tatttgttaa gtaggtaatt
     2521 actacatgag caccttcctt tgcaaaacct tttgcaatct cttttcctat accactgcta
     2581 gaaccagtta taatgcatac tttatctttt aatctcattg tttttcatat gtttctct
//
LOCUS       DTVV01000232            2368 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1664933, whole genome shotgun sequence.
ACCESSION   DTVV01000232 DTVV01000000
VERSION     DTVV01000232.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1664933"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="EYO86_02760"
     CDS             <1..310
                     /locus_tag="EYO86_02760"
                     /inference="COORDINATES: protein motif:HMM:PF01075.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41989.1"
                     /translation="FPKAVIPEDIIKDFSNIEIVMGSTKFINCAVANDSGIAHMLSTK
                     YCPVIKLFGHKDSNKFTPQHKNLIPINSSEFNSRDVKIIPTVRVIKEINKVIGWTIER
                     "
     gene            333..1046
                     /locus_tag="EYO86_02765"
     CDS             333..1046
                     /locus_tag="EYO86_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41990.1"
                     /translation="MFKFEKLIFGDLGWGDEFLIATLMTIIVSICSMGLGIFIAIFAA
                     WAKLTLSKFLNFISNFYTTVVRGIPELLVIYLIFFGGSAVVMKLAKVFGYNGYIELNA
                     FTISVVAIGIISATYSTEVLRAAFLSIDRGQVEAAKTLGLSKFQIFYKILVSQVLRHA
                     IPGLGNVWQVTLKDTSLISVTGLVEIMRQTRISSNVEHSPLTFLITAAFLYLFLTTFS
                     GKFFNYLERQANKGFAQTS"
     gene            1043..1759
                     /locus_tag="EYO86_02770"
     CDS             1043..1759
                     /locus_tag="EYO86_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC41991.1"
                     /translation="MNFFEFFNSFQESLIFINFFKILKALDQTLLLLLASLPIGFVLS
                     LIFALGRVSKNKLLSKTVEYYIFVIRGTPLLVQIYLIYFGLGSVKGIRDSFLWIVLKE
                     PFWCGVLALTLNTVAYGSEIFRGGIQAVSQGQIESGKSLGFTRIGLFRRIIFPIALRR
                     VLPTYGNELILMVKATSLVSLTTYMEMTGIARRIMAVTWAPVEAFLAAGILYLLLNFL
                     MVQFIKYLEWKYNPHLRVTR"
     gene            complement(1779..>2368)
                     /locus_tag="EYO86_02775"
     CDS             complement(1779..>2368)
                     /locus_tag="EYO86_02775"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HIC41992.1"
                     /translation="FADVSGSIQKDINSPLSKRPQLENHEWPEYCKKISDISSRLNDV
                     GLPMSYHEHMGTFIQTEKDVDRFINETNDNTYLLYDTGHLLFAQADYQAILKKYISRI
                     NHVHCKDIRKNVLENSLAKDLSFRESFLGGTFTVPGDGCIDYLPLLSTLHENNYNDWL
                     VIEAEQDPKKANPFEYAKIGFKYLTGIMNEVGYKI"
BASE COUNT          736 a          350 c          397 g          885 t
ORIGIN      
        1 atttcctaaa gcagtaattc ctgaggatat tattaaagat ttttctaata ttgaaatagt
       61 tatgggttca acaaagttta taaattgtgc tgtagctaat gatagtggaa ttgcacatat
      121 gctatctaca aaatattgcc ctgtaattaa actttttggc cataaagact ctaacaaatt
      181 tacaccgcag cacaaaaacc tcatacctat aaattcatct gaatttaaca gtcgggatgt
      241 caaaattatt ccaacagtaa gggttataaa ggagataaat aaagttatag gttggacaat
      301 agaacgttag atcattatta aaataaggtt aaatgttcaa atttgaaaaa ttaatctttg
      361 gtgacttggg ttggggagat gaattcttaa ttgcaacatt aatgacaatc atagtcagta
      421 tttgttcaat gggactcggt atctttattg caatttttgc agcttgggca aagttaactt
      481 tatcaaaatt cctgaatttc atttctaatt tttacacaac tgttgttagg ggcatccctg
      541 aattactagt tatatatctt attttttttg gcggaagtgc tgtcgtaatg aaacttgcta
      601 aagtttttgg ttataatgga tatattgaat taaatgcttt taccataagt gttgttgcaa
      661 ttggcattat ttctgcaact tactcaacag aagttttacg tgctgccttt ttatctattg
      721 atcgaggaca agttgaggct gcgaaaactc ttgggttatc taagtttcaa attttttata
      781 aaatattagt ctcccaagta ttgagacatg ctattccagg tttaggaaat gtttggcagg
      841 ttactttaaa agacacttcc ttaatttcag ttacaggact tgttgaaatt atgcgtcaga
      901 ctagaatttc ctctaatgtg gaacattctc ctcttacttt tttaattacc gcagcttttt
      961 tatatctgtt tctaacaact ttttctggta aattttttaa ttatcttgaa aggcaagcca
     1021 ataaaggttt tgctcaaact tcatgaattt ttttgaattt ttcaattctt ttcaagaaag
     1081 cttaatattt attaactttt ttaaaatttt aaaagctctt gatcagacgc ttttattact
     1141 actagcctct ttgcctattg gttttgtctt atctttaatt tttgctttgg gaagagtgtc
     1201 aaaaaataaa ttattatcaa aaacagttga atattatatt tttgttattc gcggaacacc
     1261 attgctagtt caaatttatc ttatttattt tggtcttggt tctgtaaaag gaattaggga
     1321 tagtttttta tggatagtat tgaaagagcc tttttggtgc ggagttttag ctctaacctt
     1381 aaatacagta gcttacggta gtgaaatttt tcgaggcgga attcaagcag tttcacaggg
     1441 tcaaattgaa tcaggaaaat ctctgggttt tacacgaatt ggactgttta gaagaataat
     1501 ttttcctata gccttaagga gagtattacc tacttatggt aacgaactaa ttttaatggt
     1561 caaggctact tctttggtta gtttaacaac ctatatggag atgacaggga tagctagaag
     1621 aattatggca gtaacttggg ctcctgtcga agctttttta gctgcaggta tattatatct
     1681 tttgcttaat tttcttatgg tgcaatttat caagtatcta gagtggaagt ataatccaca
     1741 tttaagagtg actcgataat tatacttttg actacattct atattttgta acccacttca
     1801 ttcattatgc cagttaaata tttgaaacca attttcgcat attcaaaagg attggctttt
     1861 tttggatctt gttctgcctc aataacaagc caatcattat aattattttc atgcaaggta
     1921 gataataggg gtagatagtc aatgcatcca tcacctggca ctgtaaaagt tccacctaaa
     1981 aaagattctc taaagcttag atctttagct aatgagtttt ccaaaacatt ttttctaata
     2041 tccttgcaat ggacatgatt aatacgtgaa atatattttt ttaggatagc ttggtagtct
     2101 gcttgagcaa ataatagatg tccagtatca tataaaagat acgtattatc attcgtttca
     2161 ttaataaatc tatcaacgtc tttttctgtt tgaatgaaag tccccatatg ttcatgataa
     2221 gacatgggaa gtccaacatc attaagccta cttgatatat ctgaaatttt tttacaatac
     2281 tctggccatt catgattttc caattgcggc cttttagaaa gaggagaatt aatatctttt
     2341 tggattgagc cggaaacatc agcgaaaa
//
LOCUS       DTVV01000233            1103 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1664967, whole genome shotgun sequence.
ACCESSION   DTVV01000233 DTVV01000000
VERSION     DTVV01000233.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1664967"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(26..1084)
                     /locus_tag="EYO86_02780"
     CDS             complement(26..1084)
                     /locus_tag="EYO86_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC41993.1"
                     /translation="MFSGNAYAETGKNLNWCKQNTKIKYPFYIYGEKGKIKKHKSSDA
                     LENAKNKIVIIYNYGGGNAQKYHGICRKAAKNFAGLAGHKIGNLETIWWYNDEIKKAG
                     GGGNKPFYKCKEDGPWGQDGAITDIPSNVGKHFWKCIKASVPEWNAEKRFQMMEGIIK
                     NFINAGVPPNQIFVSGVSAGGADAIRSAVHYGGKLINAGIAFHPANWGQHKSGARRTI
                     IVEEMKSVERLDLLVVNSNNDGTGARKIRPEDSQWMLEIPGVEFLVTADDGYNLMKPK
                     EYKGDFNIYTPDGKKCNHKGNWTKNSLNQEQRFLWSMDKDKISKRWDSHDMVNQKCMQ
                     YYWPQVLDYITKRIEATQ"
BASE COUNT          313 a          230 c          136 g          424 t
ORIGIN      
        1 aaatggtaaa ccgctaaaat tttaattact gagtggcttc aattctttta gttatgtagt
       61 ccaaaacttg tggccaataa tactgcatac atttttgatt aaccatatca tgtgaatccc
      121 aacgttttga tattttgtct ttgtccatgc tccaaaggaa acgttgttct tgatttaaac
      181 tatttttagt ccaattacct ttgtgattac atttcttacc atcgggagtg tatatattga
      241 aatcaccttt atattctttt ggtttcatta gattgtatcc atcatcagct gttactaaaa
      301 attcaacacc tgggatttct aacatccact gactatcttc aggtcttatt tttctagctc
      361 cagtcccatc attattagaa ttaacaacta acagatctaa tcgctcaaca cttttcattt
      421 cttctacaat aattgttctt cgggctcctg atttatgttg tccccaattt gcaggatgaa
      481 aagcgatacc agcatttatt aattttccac cataatgcac tgcggatcga attgcatctg
      541 ctcctcctgc tgaaactcca gaaacaaaaa tttgattagg tggaactcca gcattaataa
      601 aatttttaat aattccttcc atcatttgaa atcttttttc agcattccat tctggtactg
      661 atgctttaat gcatttccaa aaatgtttac caacatttga aggaatatct gttattgccc
      721 catcttgtcc ccaagggcca tcttctttac atttgtaaaa aggtttattt cctccacctc
      781 cagctttttt gatttcgtcg ttataccacc atatagtttc taaatttcca atcttgtgtc
      841 ctgctaaacc tgcaaaattt ttagcagcct ttctacaaat gccgtgatat ttttgtgcat
      901 ttccaccacc ataattgtaa ataattacaa ttttattttt tgcgttttct agagcatcac
      961 tagatttatg tttttttatt tttccctttt ctccataaat ataaaaagga tacttgattt
     1021 tagtattttg tttacaccaa tttaaatttt ttcctgtctc tgcataagca ttcccactaa
     1081 acaacaaacc cagaaccaag atc
//
LOCUS       DTVV01000234            2258 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1670410, whole genome shotgun sequence.
ACCESSION   DTVV01000234 DTVV01000000
VERSION     DTVV01000234.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1670410"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="EYO86_02785"
     CDS             <1..1105
                     /locus_tag="EYO86_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIC41994.1"
                     /translation="IIAEKNGFIKYLDLKQGISFRDTVDDTTGISSKIIIDWSQNPKS
                     KNFKPSINIVKSYDDEIDAEDTNVFSYPMSIDTILSIEDGAKVKAGQVIARIPKESSK
                     TKDITGGLPRVAELFEARKPKDPAIMSEIDGKISFGKDFKNKRRLIITSLDDNENFEI
                     LIPRSKYLNVQNGDFVKKGDILVEGTPVPHDILRILGIEELAKYLVREVQSVYKLQGV
                     YINDKHIETIARQMLQKGLIKDAGDSGLLVGEQKQKKDIFEINKILENDGKKPIVYED
                     VLLGITKASLQTDSFISAASFQETTKVLTDAATQGKTDYLTGLKENVIVGRLIPAGTG
                     SMTTTYTKLAHSRDMAKLAEKKKIQENSENLEQ"
     gene            1212..1583
                     /locus_tag="EYO86_02790"
     CDS             1212..1583
                     /locus_tag="EYO86_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIC41995.1"
                     /translation="MPTINQLVRNGRQVVKKRNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVARVKLTNGHEVSAYIPGEGHNLQEHSVVLLRGGRVKDLPGVRYHILRGTL
                     DTQGVTSRKQRRSLYGTKKPK"
     gene            1594..2064
                     /gene="rpsG"
                     /locus_tag="EYO86_02795"
     CDS             1594..2064
                     /gene="rpsG"
                     /locus_tag="EYO86_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIC41996.1"
                     /translation="MSRRRAAIKRTILPDHKFKDLVIAKFMNRIMLDGKKSVSEKIVY
                     GAFDIVSKKTDKEPLEFFHEALDNVKPNLEVKSRRVGGATYQVPMEVRPRRAQTLAMK
                     WIVDCAVKRNEKTMRERLASEIIDAHNNKGNAVKKREETHKMAEANRAFSHFRW"
     gene            2071..>2258
                     /locus_tag="EYO86_02800"
     CDS             2071..>2258
                     /locus_tag="EYO86_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIC41997.1"
                     /translation="MSRAHPLEKYRNIGIMAHIDAGKTTTTERILYYTGKSHKIGEVH
                     DGAAIMDWMEQEQERGIT"
BASE COUNT          874 a          316 c          440 g          628 t
ORIGIN      
        1 aattattgca gaaaaaaatg gattcatcaa atatttagat ctcaaacagg gcatctcatt
       61 tagagacaca gttgatgata caacaggtat ttctagtaaa attattatcg attggagcca
      121 aaatccaaaa tcaaaaaatt ttaaaccatc aataaatatt gtaaaatcat acgatgatga
      181 aattgacgct gaagatacaa atgttttttc ttatccaatg tcaattgata caattcttag
      241 catagaagat ggagctaaag ttaaagcagg tcaagtaata gcaagaattc ccaaagaatc
      301 ttctaaaacc aaagatatta ctggtggcct ccctcgtgtg gccgagctat ttgaggcaag
      361 gaagcctaaa gatcctgcta ttatgagtga aattgatggg aaaatttcgt ttggtaaaga
      421 ttttaaaaat aaaagacgat tgataataac tagtttggac gacaatgaaa actttgaaat
      481 attaattcca agatcaaaat atttaaatgt tcaaaacgga gattttgtaa aaaaaggaga
      541 tattttagtt gaaggaacgc ctgtacctca tgatatactg cgtattttgg gaatagaaga
      601 actcgcaaaa tatctcgtta gagaagttca atcagtttat aagttacaag gtgtttatat
      661 taatgataag cacatagaga caatagcgag gcagatgttg caaaaaggcc ttattaaaga
      721 tgcaggtgac tcaggtttat tggttggaga gcagaagcaa aagaaagata tttttgagat
      781 taataaaata cttgaaaatg atggaaaaaa accgatagtt tatgaagatg ttctcttagg
      841 tattactaag gcgagtttac aaacagattc tttcatttcg gcagcttcct ttcaagaaac
      901 cactaaagtt ctaacagatg cagctacaca aggtaaaact gactatctca ctggattaaa
      961 agaaaatgtt attgttggca gattgatacc agccggaaca ggaagcatga ctactactta
     1021 tactaaatta gctcactcta gagatatggc taaacttgct gaaaagaaaa aaatacaaga
     1081 aaatagtgaa aatctagagc aataattacc tttattctct tgacttatgt agtccttata
     1141 aataaatacg cgctactttt ggtcaatggt agtggaaaat tcatttcaaa cgcttgacag
     1201 aggaatagta aatgccaact ataaatcaac ttgttcgcaa tggtaggcaa gtagtaaaaa
     1261 aaagaaataa agttcctgcg ttagaagctt gtcctcaaaa aagaggtgtt tgcacaagag
     1321 tttatactac tactcccaag aaacccaatt cagcattacg taaagttgca agggttaagc
     1381 ttactaatgg acacgaagtt tcagcatata ttccaggaga aggccataat cttcaagaac
     1441 attcagtagt tctattgaga ggtggtagag tgaaggattt accaggggta agataccata
     1501 ttttgagagg tacattagat actcaaggag taacctcaag aaaacaaaga cgttcacttt
     1561 acggaacaaa aaaacctaaa taattacaat aaaatgtcta gaagaagagc agcaataaaa
     1621 agaactattt taccagatca taaatttaaa gatttggtca tagctaagtt tatgaacaga
     1681 attatgcttg atggaaaaaa atcagtttct gaaaaaattg tttatggagc ttttgatatt
     1741 gtttccaaaa agactgataa ggaaccatta gaattttttc atgaagcttt ggataatgtg
     1801 aaacctaatt tagaggttaa atctagaagg gttggtggag caacttatca agttcctatg
     1861 gaagttagac caagaagagc ccagaccctt gcaatgaaat ggattgtaga ttgcgctgtc
     1921 aaaagaaatg aaaaaacgat gagggaacgt ttagcaagtg agattattga tgctcataat
     1981 aataaaggta atgcagttaa aaaaagagaa gaaactcata agatggcaga agctaataga
     2041 gcgttttctc attttagatg gtaggttgat atgtctagag cccatccatt agaaaaatat
     2101 agaaacatcg gtattatggc tcatattgat gctggtaaaa cgacaacaac agaaagaatt
     2161 ttatattata ctggaaaatc tcataaaatt ggtgaggtgc atgacggagc tgcaataatg
     2221 gattggatgg aacaagaaca agaacgtggt atcacaat
//
LOCUS       DTVV01000235            2033 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1670791, whole genome shotgun sequence.
ACCESSION   DTVV01000235 DTVV01000000
VERSION     DTVV01000235.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1670791"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="EYO86_02805"
     CDS             <1..361
                     /locus_tag="EYO86_02805"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HIC41998.1"
                     /translation="QINQFLSQRIKISNEYKNKIKTLTFQKTPQYVTTHSHMLFFAFS
                     KNKNERDRNLKFLRDNGIDARLPYMPIHKQPCNFELESSDAPNSNLIYETAFTLPIFN
                     NMTEDECNLVVDYCNKL"
     gene            371..1624
                     /locus_tag="EYO86_02810"
     CDS             371..1624
                     /locus_tag="EYO86_02810"
                     /inference="COORDINATES: protein
                     motif:HMM:PF08421.9,HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC41999.1"
                     /translation="MTLEPRKTCRICNSDQLTTIMSLGEQYIAAYTPKDTDPEPILDK
                     FPLELVRCTASHCGLVQLRHSTPSDILYERYFYRSGINKTMIENLNEVVTQAMSKVQL
                     NENDIVLDIGCNDGTLLKNYQAKKLRLVGIDPAKNMAKFSKETGAHIIVDYFSAKSFV
                     EHYNHEKAKIITSVAVFYDLEDPNQFVSDIAKILDENGVWVIELKYLPTMIFQNAFDN
                     IVHEHLEYYHFAVIEHMLEKYSLKAIDALLNDSNGGSVRIFVKHASQPIDEESEKHLS
                     ELRRSEEAMNLSTDRPYEEFLHRCEKLKEQTVSFIKKEVNAKKKIHVYGASTKGNTIL
                     QYYGLDDNLIDAIADRNPDKWSRKTIGTNLKIISEEESRSLNPDYYFILPWHFLEEFR
                     QREKEFFKNGGKFIVPLPDFKIIDK"
     gene            1631..>2033
                     /locus_tag="EYO86_02815"
     CDS             1631..>2033
                     /locus_tag="EYO86_02815"
                     /inference="COORDINATES: protein motif:HMM:PF16363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIC42000.1"
                     /translation="MFENKNILITGGTGMIGSHLVELLRDKSNVRIISHKRKIPMQIN
                     SKNIEILNGDLTDKEFAIKAVKGMDFVFHLAAYTGGLGRTTVYPASTLTPNLILDGNI
                     IDSAKNENVERLLYASCSCVYPDLEKDFSEED"
BASE COUNT          784 a          276 c          319 g          654 t
ORIGIN      
        1 acaaattaat caatttcttt cacaaagaat taaaatttcc aatgaatata aaaataaaat
       61 taaaacttta acttttcaaa aaactcctca atatgtaaca actcactctc atatgctttt
      121 ctttgcattc tcaaaaaata agaatgaaag agatcgaaat ttgaaatttc taagggataa
      181 tggaatagat gcccgtttac catatatgcc aatacataaa caaccatgca attttgaact
      241 tgaaagttct gatgctccta actctaattt aatttatgaa acagcattca cacttccgat
      301 ttttaataat atgactgaag atgaatgtaa tctagtagta gattattgta ataaactata
      361 gaaaatcatt atgactttag aaccacgtaa aacatgtaga atctgtaact ctgatcaact
      421 tacaactatt atgtcattgg gtgaacagta tattgcagca tatactccaa aagatactga
      481 tcccgaacct atactagata aatttccctt agagttagtc agatgcaccg catctcattg
      541 tggtttagta caattacgcc attctacacc atctgatatc ttgtatgaac gctatttcta
      601 tagatctgga attaataaaa ctatgattga aaatctgaat gaggttgtta cacaagcaat
      661 gtcaaaagta cagttgaatg aaaatgatat tgttttagat attggttgca atgatggcac
      721 cttgttaaaa aattatcaag caaaaaaatt acgtctagta ggtattgatc cagctaaaaa
      781 tatggctaaa ttttcaaaag aaacaggagc tcatattata gtagattatt ttagtgcaaa
      841 atcttttgtt gaacattata atcatgaaaa agccaaaatt attaccagtg tagctgtatt
      901 ctatgatctt gaagatccta atcaatttgt gtctgatatt gcaaaaatat tagatgaaaa
      961 tggagtttgg gtaatcgaat taaaatactt gccaactatg atttttcaaa atgcttttga
     1021 taatattgta catgaacatc tagaatacta tcattttgca gttatagaac atatgttaga
     1081 aaaatatagt ttgaaggcaa tagatgcttt acttaatgat tctaatggtg gcagtgtacg
     1141 aatttttgta aaacatgcaa gtcaaccaat cgatgaggaa tcagaaaaac atctatctga
     1201 attgagacgt tctgaagaag cgatgaatct tagtacagat agaccttatg aagaatttct
     1261 tcatagatgt gaaaaattaa aggagcagac agtatctttt attaaaaaag aagttaatgc
     1321 aaaaaaaaaa atacacgtgt atggtgcctc aacaaaaggt aatacaattt tacaatatta
     1381 tgggttggat gataatctta ttgatgctat tgcagacaga aatccagata aatggtcccg
     1441 caaaactata ggaactaatt taaaaattat ctcagaggaa gaatctagat ctttaaatcc
     1501 tgattattac tttattttac cttggcattt tctagaagaa tttagacaaa gagagaaaga
     1561 attttttaaa aatggcggaa aatttattgt tcctttacct gattttaaaa ttattgataa
     1621 atgatataaa atgtttgaaa ataaaaacat cttaattact ggaggaactg gaatgatagg
     1681 ttcccatcta gtggaattac ttagagataa atctaacgtg agaataattt cacataaaag
     1741 aaaaattcct atgcaaatta attcaaaaaa tatagaaatt ctcaacggag atttaactga
     1801 taaagaattt gcaataaaag cagttaaagg aatggatttt gtatttcatt tagcagccta
     1861 tactggtgga ttaggaagaa caactgtata tcctgctagt acattaacac cgaatttaat
     1921 tttagatggg aacatcattg attcggcaaa aaatgaaaat gttgaaaggc ttttgtatgc
     1981 tagttgctca tgtgtctatc ccgatttaga aaaagatttt tcagaagaag atg
//
LOCUS       DTVV01000236            4289 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1673377, whole genome shotgun sequence.
ACCESSION   DTVV01000236 DTVV01000000
VERSION     DTVV01000236.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1673377"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="EYO86_02820"
     CDS             <1..246
                     /locus_tag="EYO86_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIC42001.1"
                     /translation="PILGIQDVNNDERIRFIAGCHGLEALEKKVNDNPDSVAFSIFPT
                     HIDDVIEVANQNLTMPPKTTWFDPKPLDGLVVYEFNQ"
     gene            252..1412
                     /locus_tag="EYO86_02825"
     CDS             252..1412
                     /locus_tag="EYO86_02825"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HIC42002.1"
                     /translation="MIKKPDKKPSNPNFSSGPTTKRPNWLINNLEGALLGRSHRAGEC
                     KERLKEVITKSKSLLNLPEDYLVGIMPGSDTGALEAAMWSLLGNRGIDVLAWENFGKE
                     WVIDVVDELKIEDRNLHLSDYGTLPDLTKVNFSRDVIFTWNGTTSGVRVPNGDWIPDD
                     REGLTICDATSAIFAMPIDYRKCDVLTWSWQKVLGGEAGHGMLALSPRALQRLQTYQP
                     PWPIPKVFRIASKKKLISGIFEGNTINTPSMLCVEDAIDGLNWSEEIGGLDELFRRSN
                     TSLSLIETWVQNISWVDFLNADKQTRSNTGITFKIVEDWFVKKEEKQQRDIMKEITSL
                     LTKENIAYDINGYPKAPPSFRIWGGGTVEPDNIKNLLPWIDWAYHQVKGNFA"
     gene            1405..2340
                     /locus_tag="EYO86_02830"
     CDS             1405..2340
                     /locus_tag="EYO86_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIC42003.1"
                     /translation="MPKILISDALSNLANEVFERNNIEVDTQTDLTPSELKDIINQYD
                     GLVVRSATKATDEIISAGSKLKIIGRAGAGVDNIDLDSAKKNNVIVMNTPGGNTNATA
                     EHTLSLLLSLYRSIPDASCGTHQGLWEKKKFKGLELKGKTLGIIGFGNVGARFAEMAF
                     ALGINVTVCSESFPSRQKDFPNFASIDLSELISNSDIISFHCKAPKDGKAIISMSQLK
                     LMKKKSVIINTARGGLVDEIDLKKALEEGIIRGAALDVFSNEPAKSNVLFDAPNLILT
                     PHIAASTVEAQLVVAEQIAEQISDYFNKGEVKNSV"
     gene            complement(2337..4202)
                     /gene="htpG"
                     /locus_tag="EYO86_02835"
     CDS             complement(2337..4202)
                     /gene="htpG"
                     /locus_tag="EYO86_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIC42004.1"
                     /translation="MADTKNNKESLVFQAEVSKLLDLMANSLYSEREIFLRELISNSA
                     DACEKLRYEALSKKGLLGKEKELKINIRVSKKKKIIEVEDNGIGMSRQDLIDNLGTIA
                     RSGTNKFIEAMKTKKGDDLSSIGQFGVGFYSSYMVADSVKVLSKSAELEESFLWSSKG
                     KDNYIVESFSKENRGTIITLNIKKDSDEFLDSFRLRSIITKYSNYIPFPIYLKDLDNK
                     EKEEKINEGNPLWLKDKKNIKPEDYKQFYNNISFNFDEPLKTIHYNAEGVINYRALLY
                     IPTNQPMDLFQQDRKNKIKLYVQKVFITDDCDEIIPNWLRFIPGVIDSQDISLNISRE
                     MLQNNPVITKIKKGITTKILNEITTFSSKEVDTYHKFWSNFGAVIKEGLYEFNDHHDK
                     ILPLLRFQTSESEDLISLDDYLKKMKSDQKEIYYFANTDKDHIKNSPQLEGFIANKIP
                     VLFMTDAVDEFWLQNIQKFKEKEFKSITKGKVDLSKLKKDSDKKDNINKNVTKINDLI
                     NVLKKELEAKISNVIISDRLTKSPILLVADETSMDINMEKLMKMHNKKTQDSKKILEI
                     NPDHPMIIKLSETLTKVDHKKLSNLLLDQANILDGNVLSNPTNYIESLTDLFIKP"
BASE COUNT         1422 a          629 c          727 g         1511 t
ORIGIN      
        1 cctatattag gtattcaaga tgtaaataat gatgaaagaa taagatttat tgcaggttgt
       61 catggacttg aggctcttga aaaaaaagtt aatgataacc ctgatagtgt tgcattttct
      121 atatttccta ctcatattga tgatgttata gaagttgcta atcaaaattt aactatgcca
      181 cctaaaacaa cttggtttga cccaaaacct cttgatggtc ttgtggttta tgaatttaat
      241 caataaatat aatgataaag aagcctgata aaaaaccatc caacccaaat ttttcaagtg
      301 gtccgaccac taaaagacca aattggttaa ttaataattt agagggagca ttattaggca
      361 gatctcatag agcaggtgaa tgtaaagaaa gattgaagga agttataaca aaatcaaaat
      421 cactactaaa tttacccgaa gattacttag tcggtattat gcctgggtcc gatacaggag
      481 ctttagaagc agctatgtgg agtttgttgg gaaatagagg aatagatgtt ttggcttggg
      541 aaaattttgg caaggaatgg gtaattgatg ttgttgatga actaaaaata gaagatagaa
      601 atttacacct atctgattac ggtacattgc ctgatttaac caaagtaaat ttttctcgtg
      661 acgtgatttt tacatggaac ggcaccacat caggagttcg tgtgcccaat ggtgactgga
      721 taccggatga tagggaaggt ttgacaatat gtgatgccac ttcggccatt tttgccatgc
      781 ccatagacta caggaaatgc gatgttctca cctggtcctg gcaaaaggtt ctaggcgggg
      841 aggctggtca cggcatgtta gcattatctc caagagcatt acagagatta caaacttatc
      901 aacctccatg gcccatacct aaagttttta gaattgcgag taagaaaaaa cttatttcag
      961 gaatatttga aggaaacaca ataaataccc cttcaatgct atgtgttgaa gatgccattg
     1021 atggattaaa ttggtcagaa gaaataggag gtcttgatga attatttagg agatcaaata
     1081 cctctctttc tctcattgaa acatgggtgc aaaatatttc ttgggttgat tttttaaatg
     1141 cagataaaca aactagatct aatactggta tcacttttaa aattgttgaa gattggtttg
     1201 ttaaaaaaga ggaaaaacaa caaagagata ttatgaagga aatcacttca ttattaacaa
     1261 aagaaaatat agcctatgat attaatggtt acccaaaagc tcctccatca tttagaatat
     1321 ggggcggtgg aactgtagaa cctgacaata taaagaatct cttgccttgg atagattggg
     1381 cttaccatca agtgaaagga aactttgcct aaaattctta tatccgatgc gttaagcaat
     1441 cttgcaaatg aagtttttga gagaaataat attgaggttg atacacaaac agatttaaca
     1501 cctagtgagc ttaaagatat tattaaccaa tacgatggat tagtagtacg ttcagcaacc
     1561 aaagcaacag atgaaattat ctcagcggga tcaaaattaa aaattatagg aagagcgggg
     1621 gcgggggttg ataatattga tttggactct gctaaaaaaa ataatgttat agtaatgaat
     1681 accccgggag gtaacacaaa tgctacagca gaacatacac tatcattgtt attatcttta
     1741 tataggagca tcccagatgc aagttgtgga acacatcaag gtttgtggga aaaaaagaaa
     1801 tttaaaggat tggaactaaa aggtaaaact ttgggcatta taggttttgg gaatgtaggc
     1861 gctagatttg ctgaaatggc ttttgctctt ggtattaatg ttactgtttg ttcagagtca
     1921 tttccatctc gacaaaaaga tttccccaat tttgcatcaa ttgatttatc tgaattaata
     1981 tcaaattcag atataataag ttttcattgt aaagccccaa aggatgggaa agctattatt
     2041 tcaatgagcc aattaaaatt aatgaaaaaa aaatctgtaa taattaatac agcaagaggc
     2101 ggattggttg acgaaattga tttaaaaaaa gcattagaag aaggtattat tagaggagcc
     2161 gctttggatg ttttctcaaa tgagccggct aaaagtaatg ttctttttga tgcacctaac
     2221 cttattttga ctccacatat tgctgcttct actgttgaag ctcaattagt agttgcagag
     2281 cagatagcag agcaaatatc ggattatttt aacaaaggtg aagttaaaaa ttcagtttag
     2341 ggtttaataa ataaatctgt gagtgattca atataatttg tagggtttga aagaacgttt
     2401 ccatctagaa tatttgcctg atctaacaat aagttagata attttttatg atcaacctta
     2461 gttaacgttt cagaaagttt aataatcatg ggatggtctg gattaatttc taagattttt
     2521 ttactatctt gtgttttttt gttatgcatt ttcattaact tttccatatt aatatccatg
     2581 ctagtttcgt ccgctacaag caaaatcggg cttttagtta atctgtctga gataattacg
     2641 tttgatattt ttgcttcaag ttcttttttt aatacattaa ttaaatcatt gattttagtt
     2701 acatttttat taatattatc ctttttatct gaatctttct taagtttaga taaatctacc
     2761 tttccttttg taatggactt gaattctttt tctttgaatt tttgaatgtt ttgaagccaa
     2821 aattcatcta cggcatcggt cataaacaac accggtattt tattagcaat aaaaccctct
     2881 aattgaggac tgtttttaat gtggtctttg tctgtatttg caaaataata tatttctttt
     2941 tgatcagatt tcattttttt taaataatca tcaagagaaa ttaaatcttc cgattccgaa
     3001 gtttgaaagc gaagtaaagg tagaatttta tcatgatggt cattaaattc atacaaacct
     3061 tcttttatga ctgctccaaa attgctccag aatttatgat aagtatctac ttctttggaa
     3121 gaaaaagtag ttatttcatt taaaatttta gttgttattc cttttttaat ttttgtaatt
     3181 acagggttat tttgaagcat ttccctacta atattcaaag aaatatcttg agagtcaatc
     3241 acaccgggaa taaatcgtag ccaattaggt ataatttcat cacaatcatc agtaataaaa
     3301 actttttgta catatagttt gattttgttt tttctgtctt gttggaatag atccataggt
     3361 tgattagttg gaatatataa aagagctcta taatttatta ctccttcagc gttgtagtga
     3421 atagttttta atggttcatc aaagttaaaa gatatgttgt tgtaaaattg cttataatct
     3481 tcaggtttta tatttttttt atcctttaac cataaaggat tcccttcatt aattttctct
     3541 tccttttctt tattgtctaa atcttttaga taaataggaa agggaatata attggaatac
     3601 ttggtaatta tggatctaag tctaaaacta tctaaaaatt catcagaatc ttttttaatg
     3661 tttagagtta taatagttcc tctattttct ttactaaaag attctactat ataattatct
     3721 tttcctttag aagaccataa aaaagattct tccaattcag cgctttttga taacactttg
     3781 actgagtctg ccaccatata tgaggagtaa aaacctactc caaattgccc tatagatgac
     3841 aagtcatcac ctttttttgt tttcatagct tcaataaact tgtttgtacc agaacgcgca
     3901 atagttccaa gattatcaat taaatcttgt cgtgacatgc caataccgtt atcctctact
     3961 tcgattattt tctttttctt agaaactcgt atattaattt ttaattcttt ttcttttcct
     4021 aaaagacctt ttttggaaag tgcttcatac cgtaatttct cacaagcatc cgcggaattt
     4081 gaaattaatt cacgaagaaa tatctctctt tcactgtata aagaattagc cattaaatcg
     4141 agaagtttgc taacctcagc ttggaaaact aagctttctt tattgttttt tgtatctgcc
     4201 attatatgat ttaaaattta agcactctta ttgtatattc aataggttac aatctttttt
     4261 tgccatattt atggttatac agttaaaaa
//
LOCUS       DTVV01000237            3027 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1673596, whole genome shotgun sequence.
ACCESSION   DTVV01000237 DTVV01000000
VERSION     DTVV01000237.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1673596"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="EYO86_02840"
     CDS             complement(<1..333)
                     /locus_tag="EYO86_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42005.1"
                     /translation="MILHYFIKNGTTDKKTAELIYQKIINNVNSIILCNSKVLKKDIN
                     TTFEITSIFIISIFFGSKFKKNKDDFLTLQEIMNLFTSDLDYSFRLYGIGDMNIGKNV
                     KLYIKKFYF"
     gene            337..771
                     /gene="bamE"
                     /locus_tag="EYO86_02845"
     CDS             337..771
                     /gene="bamE"
                     /locus_tag="EYO86_02845"
                     /inference="COORDINATES: protein motif:HMM:PF04355.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIC42006.1"
                     /translation="MFKYLKVIIFILMLFIILSCVETKSYSGKLIDENFKYDSLLNKT
                     QVTSKLGQPNFIDPIENKYYYYFEKRINKSIFDSRIEKRTMIVFNFEENENVQSFNQY
                     NLNQEQQINIIKDKTKNNLIDRGLIEKIFGGIGKQKLSDNSQ"
     gene            complement(777..2870)
                     /locus_tag="EYO86_02850"
     CDS             complement(777..2870)
                     /locus_tag="EYO86_02850"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIC42007.1"
                     /translation="MQTFIILCGLAAVLYGLITSRQILSLSPGNDKMQEISKAIQEGA
                     RAYLNRQYMTIGVVGVIIFILIWLIFGLSSAAGFFIGSFFSGAAGYVGMNISVRANVR
                     TTQAASSSLAEGLSVSFKSGAVTGMLVAGFALLSIALYYFILSNSGNFEGRDIVAPLV
                     SLGFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVG
                     DCAGMAADLFETYIVTVVATMVLASIFFVGDLKDTMMMYPLSIAGVCILASIIGTYFV
                     RLGKNENIMFALYKGFFATSMLSAIFLYFITDHVIGTDSTFLIGEKSFTGISLFYCGL
                     IGLIITGLIIWVTEYYTGTNYRPVQSIAKASTTGHGTNVIQGLAISLEATALPAIIIV
                     AGIIITYSLAGLFGIAIAVTTMLALAGMVVALDAYGPVTDNAGGIAEMAKLDEVVRKT
                     TDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTEDLEHFTSDPESKLHGLNVSFDL
                     SNPYVVAGLLLGGLLPFLFSAMSMTAVGRAAGAVVIEVRRQFKEIPGIMEGKSKPEYG
                     TCVDILTKAAIKEMIIPSLLPVLSPIIVYYIILLIAGQAAALSCLGALLLGVIVTGLF
                     VAISMTAGGGAWDNAKKYIEDGNYGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPMIK
                     ITNIVALLLLSSISF"
BASE COUNT         1105 a          553 c          396 g          973 t
ORIGIN      
        1 aaaataaaat ttttttatat ataattttac attttttcct atattcatat ctcctatacc
       61 ataaagtcta aatgaataat ctaaatctga agtaaacaaa ttcattatct cttgaagagt
      121 taaaaaatca tctttatttt ttttgaattt tgatccaaaa aaaatagaaa tgataaaaat
      181 tgaagttatt tcaaaagtgg tgttaatatc tttttttaac actttagaat tacataaaat
      241 aattgaatta acattattaa ttattttttg atagataagt tcagctgttt ttttatctgt
      301 agttccattt tttataaaat aatgtaaaat cataatatgt tcaaatactt aaaagttatt
      361 attttcattc ttatgttatt tattatatta agttgtgtag aaacaaaatc atattcaggt
      421 aaattaattg atgaaaattt taaatatgac tcacttttaa ataaaactca agtaacatca
      481 aaacttggac aaccaaattt tattgaccct atagaaaaca aatattatta ctattttgaa
      541 aaaagaatta acaaaagtat atttgattct agaatagaaa aaagaacaat gatcgtattc
      601 aattttgaag aaaatgaaaa tgttcaatct tttaatcaat ataatttaaa tcaagaacag
      661 caaattaata ttattaagga taaaacaaaa aataatttga ttgatcgcgg actaatagaa
      721 aaaatatttg gaggtatagg aaaacaaaaa ctttctgaca actctcaata aattaactaa
      781 aatgaaattg aagataataa taataatgct acgatattag taattttaat cattgggtta
      841 acagctggtc ctgctgtgtc tttataagga tcccctactg tatctcctgt aactgcagct
      901 ttatgagctt cactaccttt gcctccatag ttaccatctt cgatatattt tttagcatta
      961 tcccaagcac ctcctcctgc tgtcatgctt attgcaacaa ataatccggt aacaattaca
     1021 cccagaagta aagctccaag acaagataat gcagcagctt gccctgctat taataatata
     1081 atataatata caataattgg agataaaaca ggaagcaagg atggtataat catttcctta
     1141 attgctgctt tagttaaaat atctacacaa gttccatatt ctggcttgct ttttccttcc
     1201 attattccag gtatttcttt aaattgtctc ctgacttcaa ttacaacagc tcctgcagct
     1261 ctacctacgg cagtcatact catagcacta aatagaaaag ggagtagtcc tcccaacaaa
     1321 agacctgcca caacataagg gtttgataaa tcaaaagaaa catttaaacc atgtaactta
     1381 ctctctgggt cagaagtaaa atgttcaaga tcctcagtat aagctgcaaa taaaactaaa
     1441 gctcctaatc cagcagaacc tattgcatac ccttttgtaa cagctttggt ggtattaccg
     1501 acagcatcta acgcatccgt tgtttttcta acaacttcat cgagcttggc catttcagca
     1561 atcccgccgg cattatcagt tactgggcca tatgcatcta aagctactac catacctgca
     1621 agagccaaca ttgtggttac cgctattgct attccaaata aacctgccaa agaataagta
     1681 ataatgattc ctgcaacaat aataattgct ggcaatgctg ttgcctctaa actaattgct
     1741 aatccttgga taacatttgt gccatgtcct gtggttgatg cttttgcaat actttgaaca
     1801 ggtctataat tagttcctgt ataatattct gttacccaaa taattaatcc tgttattatc
     1861 agccctatta aaccacaata aaaaagagat attccggtaa aagacttttc tccaatgagg
     1921 aatgtagagt ctgtacctat cacatgatct gttatgaaat atagaaatat agctgataac
     1981 atagacgttg caaagaagcc tttgtataaa gcaaacataa tattttcatt tttaccaagt
     2041 ctaacaaaat aggttcctat aatacttgct aaaatgcaaa caccggcaat tgaaagtgga
     2101 tacatcatca tagtatcttt caagtcacca acaaagaata tggatgctaa aaccatagta
     2161 gctacaactg ttactatata tgtttcaaat aaatccgcgg ccatgcctgc gcagtcgcca
     2221 acattatccc caacgttatc cgctattact gcaggattac gaggatcatc ttcgggaatc
     2281 cctgcttcaa ctttaccaac taaatctgcc ccaacatccg cacctttagt aaaaattcca
     2341 cctcctaatc ttgcaaaaat agatattaaa gaagcaccaa aaccaagtga aacaagtggt
     2401 gccacaatat ctcttccttc aaaattgcct gagttagata aaataaaata atacaatgct
     2461 atagaaagta acgcaaatcc agcaactagc ataccagtta ctgctccaga tttaaaagat
     2521 acagacaatc cttcagctaa acttgaacta gcagcttgag ttgttcttac attagctctt
     2581 acagatatgt tcatacctac atatcctgca gcgccagaaa aaaaggaacc tataaaaaaa
     2641 ccagctgctg aacttaaacc aaaaatcaac catattaaaa taaaaataat gactccaaca
     2701 actccaattg tcatatactg acggtttaaa taagcccttg caccttcttg aatagctttt
     2761 gaaatctctt gcattttatc attacctgga ctgagtgata atatttgtct cgatgtaata
     2821 agaccgtata aaactgctgc taagccacat aatattataa aagtttgcat tgtcgtcatt
     2881 actgattgtc tccttaaaat ttttgcatca tatgtcagaa agaatataga aaatcaagaa
     2941 attagaatga atgttgataa ggatttttaa atttcattat tttttgatca tcgacatata
     3001 ttccattttt gcctataatt ctaccta
//
LOCUS       DTVV01000238            1007 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1673933, whole genome shotgun sequence.
ACCESSION   DTVV01000238 DTVV01000000
VERSION     DTVV01000238.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1673933"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            79..501
                     /locus_tag="EYO86_02855"
     CDS             79..501
                     /locus_tag="EYO86_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42008.1"
                     /translation="MIKDLTELIKLEDQINSLGTKSEISLDQAMLNMKEVEKNLDKIS
                     GAELIKDKITKSRRILKKNDPDMSKVLSLLNEANNIFVVEKEWRKRAKNDLLPQLNEF
                     DNAIKDTIGLRLQERLTLEQAKFVSRCRSSHKDISLNF"
     gene            complement(506..>1007)
                     /locus_tag="EYO86_02860"
     CDS             complement(506..>1007)
                     /locus_tag="EYO86_02860"
                     /inference="COORDINATES: protein motif:HMM:PF13561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC42009.1"
                     /translation="HVDPVESFPDTKWEQILNINLSSCFYTSKYVLPLMKKNKWGRII
                     NIASVHGKVASKNKSAYVASKHGMIGLTKTIALETAILAITCNAICPGWVETPLVSKQ
                     IDQRMKKNKSSRKKEEFNLLREKQPNLSFIKGNAIASLAYFLCTDEAKDITGSSINID
                     GGWTSQ"
BASE COUNT          386 a          140 c          136 g          345 t
ORIGIN      
        1 aacaataaga aaaaagctca aacaaaatac aaacgtaatg tagatgaagt atataaaaat
       61 attaaatatt ttaaattaat gattaaagat ttaacagaac tcataaaatt agaagatcaa
      121 atcaattctt taggaaccaa aagtgaaata tctttagatc aggctatgtt aaatatgaaa
      181 gaagttgaaa aaaatcttga taaaatttct ggagctgaat taattaaaga caaaattact
      241 aaatctagaa gaatattaaa aaagaatgat cctgatatgt caaaagtatt atctcttttg
      301 aatgaagcta acaatatatt tgtagttgaa aaagaatggc gaaaacgagc aaaaaatgat
      361 ttgttacctc aattaaatga atttgataat gctattaaag atacaatagg actaaggttg
      421 caagaacgtt taactttaga gcaagctaaa tttgtttctc gttgtcgatc ctcacataaa
      481 gatatttctt taaattttta attttttact gggaagtcca accaccatca atattaatag
      541 atgaccccgt aatatccttt gcttcatccg tacataaaaa ataagcaagt gaagcaatag
      601 cattaccttt tataaaagac aaattgggct gtttttctct tagtaaatta aattcttctt
      661 tttttcttga tgatttattc tttttcattc tttgatcaat ttgtttagat actaaaggag
      721 tttcaaccca accagggcat atagcattac atgtaattgc caatattgca gtttctaaag
      781 caatagtttt cgttaaacca atcatcccat gttttgaagc aacataagct gatttatttt
      841 tagaagctac tttaccatga actgatgcta tattgataat cctgccccat ttattttttt
      901 tcattaatgg caaaacatat ttacttgtat agaaacaact tgataaattt atattcaata
      961 tttgttccca cttggtatca gggaatgatt ctacaggatc aacatgt
//
LOCUS       DTVV01000239            1152 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1676019, whole genome shotgun sequence.
ACCESSION   DTVV01000239 DTVV01000000
VERSION     DTVV01000239.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1676019"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="EYO86_02865"
     CDS             <1..871
                     /locus_tag="EYO86_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIC42010.1"
                     /translation="VDLRNNPKQDEGPNNASYDGTVVSAQMAFKGITSSDFVCNAGTF
                     RPLKVICDEGSMFNPIRPAAQGIYYETDIRSYDLIWKTISHLNPEKSTAGSFASICGT
                     FMGGQHPDTGATFIIIEPQVGGWGAYDEGDGMNANFSAFHGDTFNTPAEISEARHGVY
                     VDQMRLTEEDGGEGKFRGGKGIIMDYRVRSKNAWLSVAYTRSKSLPWSLEGGREGGPN
                     YVEIIRKDGKKERYSVITVLPLEPEDVVRIHTGNGGGYGNPHDRDIKLIENDLLNEYI
                     TLEQASKYYNYKK"
BASE COUNT          398 a          159 c          227 g          368 t
ORIGIN      
        1 tgttgattta agaaacaatc ctaaacagga tgaaggtccg aataacgcct cttatgatgg
       61 aacagtagta tccgctcaaa tggcatttaa aggaataacc tcaagtgatt ttgtttgtaa
      121 tgcaggaact ttccggccat taaaagtgat atgcgatgaa gggtctatgt ttaatccaat
      181 tagaccagcg gcacagggga tttactatga aacagatatt agatcttatg acttaatttg
      241 gaaaacaatc tcacatttaa atcctgaaaa atctacagct ggaagttttg cttcaatttg
      301 tggaactttt atgggcggcc agcatccaga tactggcgct acatttataa ttatcgaacc
      361 acaggttgga ggttggggtg cttatgatga aggtgatggt atgaatgcaa atttctcggc
      421 ttttcacggg gatacgttta atacgcctgc tgaaatttct gaagcaagac acggcgtata
      481 tgttgatcag atgagattaa cagaagaaga tggaggtgaa gggaagtttc gaggcgggaa
      541 aggtatcata atggattata gagtaagatc taaaaatgca tggttatcag ttgcatatac
      601 tagatcaaaa agccttccat ggtctcttga aggaggtaga gaaggtggtc caaattatgt
      661 tgaaatcata agaaaagacg gaaaaaaaga aagatattca gtcataacag tcttaccttt
      721 ggaaccagaa gatgtagtta ggattcatac tggaaatgga ggtggttatg gtaaccccca
      781 tgatagagat attaaattaa tagagaatga tcttctcaat gaatacatta cattagaaca
      841 agcaagtaag tattacaatt ataaaaaata aattttgatt tgtcagagca agcaaaaaat
      901 tcttattatt tttttgtttt attaatttta taaattcata aaaattcatt cttttttctt
      961 ttgagagaag agctaatgtt attcccgaga aataaacata atcatactta gtgaatttag
     1021 aaattaattt attgtagttt agattattta agtagtcttt tgcagcactg ttatctctcc
     1081 aataataaaa ttgcttttca ccatttttgt aatttttgat taaataaaga ccactttcct
     1141 tatccttatg aa
//
LOCUS       DTVV01000240            1739 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1678402, whole genome shotgun sequence.
ACCESSION   DTVV01000240 DTVV01000000
VERSION     DTVV01000240.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1678402"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="EYO86_02870"
     CDS             complement(<1..641)
                     /locus_tag="EYO86_02870"
                     /inference="COORDINATES: protein motif:HMM:PF00486.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIC42011.1"
                     /translation="MSKSILIVDDDKRLRELLEDYLSEKKYKIFLCEDFADAEEILEY
                     FVFDLIIVDRMMPSGDGIDLIKTVKDKSNTPVILLTAMGETESRIAGLKIGADDYLSK
                     PFEPEELYLRIQKLLALFKNDAIDNKSFIFGDFVFNITTLQLKKNSKIVYLTEGENKL
                     LLTLINKNNAIVLREELAESDYDESELRKVDVQVTRLRQKIEENPKQPRFIKT"
     gene            complement(649..1134)
                     /locus_tag="EYO86_02875"
     CDS             complement(649..1134)
                     /locus_tag="EYO86_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIC42012.1"
                     /translation="MTDLNKLLTPLFLSDKEIRKIIELIFFSYRDFTSGPDEILDKIN
                     FGRAHHRVIYFVGKREQITIRNLLSILQITKQSLSRVLNQLVKEKYIIVSTGNDKRTK
                     NLSLTKKGMELEEKLTTIQTKRIRNVIKNAKEDDINGFKKILYAMIDKNNQIQFDKLN
                     M"
     gene            1197..>1739
                     /locus_tag="EYO86_02880"
     CDS             1197..>1739
                     /locus_tag="EYO86_02880"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037028.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIC42013.1"
                     /translation="MDKAFDKREGWIWMNGSFVPWQNATSHIITQGLHYASAVFEGER
                     AYNGKIFKSEEHTKRLFKSAKIIGMTIPYSEEEINAAKNNLVAKMNYADCYVRPVVWR
                     GSQQMGLSTSRADINVAIAAWDDWASYYKLEDKLSGLKLITSPWKRPAPDTAPYEAKA
                     SGPYIICTMSKEYAEKKGYHD"
BASE COUNT          536 a          289 c          288 g          626 t
ORIGIN      
        1 atagttttga taaagcgagg ttgttttgga ttttcctcaa ttttttgacg tagccttgtt
       61 acttgaacgt caacttttct gagttcactt tcgtcataat cactttctgc caactcttcc
      121 cttaaaacaa ttgcattgtt tttattaatc aatgttaata aaagtttatt ttcaccttca
      181 gtcaaataaa caatcttaga gtttttcttt aattgaagag tggtaatatt aaagacgaaa
      241 tcgccaaaaa taaatgattt attatcaatt gcatcatttt tgaataaagc taatagtttt
      301 tgaatgcgca agtatagttc ttctggttca aaaggtttag ataaataatc atcggcaccg
      361 atttttagtc ctgcaattct gctttctgtt tcgcccatag cagttaataa aataacaggt
      421 gtattacttt tatctttaac tgttttaatt aagtctatcc catcacccga tggcatcatt
      481 cgatcaacaa tgataagatc aaagacaaaa tactctaaaa tttcttctgc atctgcaaaa
      541 tcttcacata aaaatatttt gtattttttc tctgatagat aatcctctaa caactctctg
      601 agtcttttat catcgtcaac aattaaaatg ctttttgaca tcgtgacact acatattcag
      661 tttatcaaat tgtatttgat tgtttttatc aatcattgcg tataatattt ttttaaatcc
      721 attaatatca tcctcctttg catttttaat aacattcctg atccttttag tttgtatggt
      781 agttaatttt tcttcgagct ccattccttt tttcgttaaa gaaaggtttt tagttcgttt
      841 atcattccct gtggatacta taatatattt ttcttttacc aattgattta gtacccggga
      901 caagctttgt ttggtaatct gaagtatgga gagtaaatta cggatggtaa tttgttctct
      961 tttccccaca aaatatatta ccctgtggtg agcgcgtcca aagttgattt tgtccagaat
     1021 ttcgtcagga ccagaagtaa aatcacgata agaaaaaaaa attaattcga taatttttcg
     1081 aatttctttg tcacttaaaa acagcggagt taataattta tttaggtcag tcatattgac
     1141 ttaattatgc atcactgata ctcacttgtc attaaaatat tcaacattta tgtataatgg
     1201 acaaagcatt tgataagaga gaagggtgga tctggatgaa tgggtctttt gttccttggc
     1261 aaaatgcaac ttctcacatt atcactcaag gattgcacta tgcgagcgcg gtttttgagg
     1321 gagagagagc ctacaatggg aaaattttta aatctgagga acatactaaa cgtcttttta
     1381 aatctgctaa aattattggc atgaccattc cttacagtga ggaggagatc aatgctgcta
     1441 agaataattt ggttgcaaaa atgaattacg ctgattgtta tgtaaggcct gttgtgtgga
     1501 gaggaagtca gcaaatggga ctttcaactt caagagctga tatcaatgta gctattgctg
     1561 cttgggatga ttgggcctca tattataagc tagaggacaa attatcgggc cttaagctta
     1621 ttacttctcc atggaaacgc ccagccccag atacggctcc ttatgaagct aaagcatctg
     1681 gtccatatat aatatgtaca atgtccaagg agtatgctga aaaaaaaggg tatcacgat
//
LOCUS       DTVV01000241            1212 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1680830, whole genome shotgun sequence.
ACCESSION   DTVV01000241 DTVV01000000
VERSION     DTVV01000241.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1680830"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="EYO86_02885"
     CDS             complement(<1..429)
                     /locus_tag="EYO86_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC42014.1"
                     /translation="MFPTPIQKVSLKINITYKVLLIFTLILWLLPLLAVMMTSIRTTE
                     DILKANYWTIPTEFAVIENYGTVFKLGNLAQYFFNSLVITLPTVLGTIILSSMTGYTL
                     AVHRFKLNFLIFAMFIAGNFIPFQILMIPVRHLTINLGMYD"
     gene            complement(429..1175)
                     /locus_tag="EYO86_02890"
     CDS             complement(429..1175)
                     /locus_tag="EYO86_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC42015.1"
                     /translation="MSFPQRTDKSPQGPHLQDEDFYVAFFNNVRWLILSLLVIPLGLA
                     LALFLNQNFKGMRFIKSFFYFPFVINLVVVGLIFAWFYNPKYGLLGQAFSGLGLKPIP
                     ILSDEIYATYGIIFASFWPSIAYTMIIYITGLTGINREMIEAGRIDGASGFKMLWHII
                     LPQLKPATLIAVVITIVGALRSFDLIAIMTAGGPWGSTKVLAYKMYEEALFNFRMGYG
                     AAVATILFLLMDIYIAYFLYNLYKNERGAK"
BASE COUNT          441 a          225 c          207 g          339 t
ORIGIN      
        1 atcatacatt cctaaattta ttgtgagatg tcttaccgga atcatcagta tctgaaatgg
       61 tataaagttt cctgcaataa acatagcaaa aattaaaaag tttaatttaa atcgatgtac
      121 agccaaagta taacctgtca tggaacttaa aataattgtt cctaaaactg taggaagagt
      181 aataacaaga ctgttaaaaa agtactgggc taaattgccc aatttaaaaa cggtgccata
      241 attttcaatt acggcaaatt ctgttggaat agtccaataa ttagccttca aaatatcttc
      301 agtggtacga atggaagtca tcattacagc aagcaaaggc agtaaccaaa gtataagagt
      361 aaaaataagt agtactttgt atgtaatgtt gattttaaga gatacttttt gaatgggtgt
      421 cggaaacatt attttgcccc tctttcattt ttgtataaat tatacaaaaa atacgcaatg
      481 tagatatcca ttagcaaaaa caatattgtt gcaaccgcgg ctccatatcc cattcgaaaa
      541 ttaaacagcg cttcctcata cattttatag gcaagtactt tagtactgcc ccaaggccct
      601 ccagctgtca tgatggctat taaatcaaaa cttctaagtg cgccaacaat ggttattaca
      661 actgctatca aagtggccgg ttttaattgc ggaagaataa tgtgccaaag catcttaaat
      721 ccagatgccc cgtcaattct tcccgcttca atcatctcac gattaatgcc agtcaagccc
      781 gttatgtata tgatcatggt ataagcaata gacggccaaa aagaagcgaa tataattccg
      841 taagttgcgt agatctcatc agacaggata ggaatgggtt taagtccaag accgctaaaa
      901 gcttgcccaa gaagaccata ttttggatta taaaaccaag caaatataag gccaacaacg
      961 accaaattga ttacaaatgg aaaataaaaa aaagacttga taaatcgcat tcccttgaaa
     1021 ttttgattta aaaaaagtgc tagtgctaaa cctagaggaa taactaaaag actgagtatt
     1081 agccatctca cattattaaa aaaagctaca taaaaatctt catcttgcaa gtgaggacct
     1141 tgaggacttt tatccgtcct ttgtgggaac gacagcccgt gcggaaacga atgtcatcga
     1201 ctaccagttc ca
//
LOCUS       DTVV01000242            1021 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1682921, whole genome shotgun sequence.
ACCESSION   DTVV01000242 DTVV01000000
VERSION     DTVV01000242.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1682921"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="EYO86_02895"
     CDS             complement(<1..196)
                     /locus_tag="EYO86_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HIC42016.1"
                     /translation="MFVATKYPQRAVSAALALVIIGSIAFHFWTPWWWTEIASNWGGM
                     DDTIILTFWVTGTVFCAVCLF"
     gene            414..848
                     /locus_tag="EYO86_02900"
     CDS             414..848
                     /locus_tag="EYO86_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HIC42017.1"
                     /translation="MKYLFIILFGLFIANVSFAEDTTIEMLNKLDKRTMVFSQEIVRI
                     DPGDTVFWKATNPGHNVQFISKNGVPAGVEKFKSKVGKDTEFTFTVPGIYAYWCVPHK
                     TLGMIGFIIVGNDLSNLDSIKKVKFIGKSKKIAKGLIAEIEG"
BASE COUNT          362 a          149 c          163 g          347 t
ORIGIN      
        1 tgaagagaca aacagcacaa aaaactgttc cggttaccca aaaagttaaa atgatagtat
       61 cgtccatgcc gccccagttt gacgctattt cagtccacca ccaaggggtc caaaaatgaa
      121 aagcaattga gcctataatt actaaggcta aggctgccga taccgccctt tgagggtatt
      181 ttgtcgcaac aaatattcca attgcagcca acactaaaac tacggctatg gttatgatca
      241 tatttttgta tgcatatata ttaaatatat gattggtata gtcactttct acactttttt
      301 ttactttaag tatgcgacct atggtcttaa tgacaataat ccatagccat attaattaac
      361 ttatacactt gttcatatat aagaaaaaat aattaaattt tataggagaa aaaatgaaat
      421 atttattcat aattttattt ggtttgttta ttgccaatgt atcctttgca gaggacacta
      481 ctattgaaat gctaaacaaa ctagacaagc gcactatggt ctttagtcaa gaaattgtaa
      541 gaattgatcc gggtgatact gttttttgga aagccactaa tccaggacat aatgtacaat
      601 ttatatctaa aaacggtgtt cctgctggag tagaaaaatt taaatccaaa gttggaaaag
      661 atactgaatt tactttcacc gtaccgggta tttatgcata ttggtgtgtt cctcataaaa
      721 ctttaggtat gataggtttt ataattgtag gtaatgattt atcaaatttg gattcaatta
      781 aaaaagttaa gtttattggc aaatctaaaa aaatcgcaaa aggacttatt gctgaaattg
      841 aagggtgata agctattgat acatgaaata taaaaaaaat agatttatca ttttttatgt
      901 ttttaatatt attttgctat ttgttgttct gtttttagca gttcctgcct ctaaattatt
      961 aaaaaaaata aatttagatg ataatgaatt tgttacaggg caacagatta tcaatcctaa
     1021 a
//
LOCUS       DTVV01000243            2204 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1694181, whole genome shotgun sequence.
ACCESSION   DTVV01000243 DTVV01000000
VERSION     DTVV01000243.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1694181"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            58..1113
                     /locus_tag="EYO86_02905"
     CDS             58..1113
                     /locus_tag="EYO86_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HIC42018.1"
                     /translation="MKKIKGPAIFLAQFAGDEEPFNSLDSIAKWASGLGYKGVQIPSW
                     DDRLFDLAKASDSKDYCDEIKGTLKNYNLELTELSTHLQGQLVAVHPAYDVAFDGFAA
                     ENVRGNPKARQEWAVDQMMKAAKASKNLELTAHATFSGALAWPYLYPWPQRPAGLVEE
                     AFDELAKRWKPLLDVFDNAGVDLCYEIHPGEDLHDGVTFEMFLERVNNHKRCNMLYDP
                     SHFVLQHLNYLDHIDIYYDKLKMFHVKDAELNPTGKQGVYSGFQPWINRAGRFRSLGD
                     GQVDFKSIFSKLSGYGFDGWAVLEWECCIKSPKQGAKEGAIIIQDHIIEVTEKAFDDF
                     ADSGMDKEANKKILGIK"
     gene            1113..>2204
                     /locus_tag="EYO86_02910"
     CDS             1113..>2204
                     /locus_tag="EYO86_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIC42019.1"
                     /translation="MPRRLRLGMIGGGQGAFIGAVHRIASRIDDRYDLLAGCLSSTAD
                     RALASAKEIGIDPSRSYPDFKTMAKEEAQREDGVEVVSIVTPNHMHAGPSIEFLKQDV
                     NVICDKPLTATMEDAHSLYKAVKESKAKFFLSHNYTGYPVVREMRRIVQEGTLGKIRV
                     LKGSYLQGWLGKKEEDTGENKQAEWRTDPKRSGAAGAVGDIGSHTMHLLEFVTGLKVQ
                     EVAADLTTFVEGRKLDDDASILIRLNNKAKGSLSISQVAIGEENNLKISIYGERGAIH
                     WEQENPNFARMSIDGKLDQIITRGGPIHKESSMANIRIPPGHPEGYLEGFAQIYSDAA
                     DIIQNIGNAKELIKILPNIEEGLHIMKFIN"
BASE COUNT          773 a          310 c          479 g          642 t
ORIGIN      
        1 ataatataag tggtgactta aaagtttttt aatgttttat aatttgggag aggaagaatg
       61 aagaaaatta aagggccagc cattttttta gcgcaatttg ctggagatga agaaccattt
      121 aattcattgg atagcatagc aaaatgggca tctggactag gttataaagg agtacagata
      181 ccttcatggg atgacaggtt atttgattta gcaaaagcat cagatagcaa agattactgt
      241 gatgagatca aaggcactct aaaaaactat aatttagaac taacagaact ctctacacat
      301 ttacaaggac agttagtagc tgtgcatcca gcatacgacg tcgcctttga tggttttgct
      361 gctgaaaatg taaggggcaa tcccaaggca cgtcaagaat gggcagttga tcaaatgatg
      421 aaagccgcaa aagcgtcaaa gaatttagaa ttgactgcac acgcaacttt ttcaggtgca
      481 ttggcttggc cctatcttta tccttggcca caacgaccag ctggtttagt tgaagaagca
      541 tttgatgaat tggctaaaag atggaagcca ttattagatg tctttgataa tgcaggagtt
      601 gatttgtgtt atgaaataca tccaggcgaa gatttacatg atggcgttac ctttgagatg
      661 tttttggaaa gagtgaataa tcataaaaga tgcaatatgc tttacgaccc aagtcacttt
      721 gtgcttcaac atttaaatta tttggatcac attgatattt attatgataa attgaaaatg
      781 tttcatgtca aagatgctga attaaatcca acagggaaac aaggtgtcta tagtggattt
      841 caaccttgga ttaatagagc tggtagattc cggtcccttg gggatggaca agtcgatttt
      901 aaatctattt tttctaaact ttcaggatac ggatttgatg gatgggcggt attagaatgg
      961 gaatgttgca taaaaagtcc taagcaagga gcaaaagagg gcgctattat aattcaagat
     1021 catattattg aggtaacaga aaaagctttt gatgattttg ctgattcagg aatggataaa
     1081 gaagcaaata aaaaaatact tggcataaaa taatgcctag aagattgaga ttgggcatga
     1141 ttggaggggg tcaaggcgcc tttattggtg ctgttcaccg tattgcttca agaattgatg
     1201 atagatatga tcttttagca ggttgtttat cttctactgc tgatagggct ttagcatctg
     1261 ctaaagaaat aggcatcgac ccaagcagaa gttatccaga ttttaaaaca atggcaaaag
     1321 aagaggcaca acgagaagac ggtgtagaag ttgtatcaat cgttacacca aatcacatgc
     1381 atgctggacc ttctattgaa tttttaaaac aagatgtaaa tgttatttgt gataagccgc
     1441 taactgctac tatggaagat gctcattctc tttataaagc agtaaaagaa tcaaaagcaa
     1501 agtttttttt atctcataat tatactggtt atccagttgt tagagaaatg agaagaattg
     1561 ttcaagaagg cactttaggc aaaattaggg ttcttaaagg atcatattta caaggatggc
     1621 ttggtaaaaa agaagaggat acaggtgaaa acaagcaggc tgaatggaga actgatccca
     1681 aaagaagcgg agctgctggt gcagtgggag atattggtag tcacacaatg catcttttgg
     1741 aattcgtaac aggattaaaa gttcaagaag ttgccgctga tttaacaaca tttgtagaag
     1801 gaagaaaatt agatgatgat gcaagtattt taataaggtt aaacaacaaa gctaaaggaa
     1861 gcttatcaat ttctcaggtt gctataggag aagaaaacaa tttaaaaatt tctatttatg
     1921 gagagagagg cgctattcac tgggaacaag agaatccaaa ttttgctaga atgagtattg
     1981 atggtaaact tgatcaaata ataacaagag gaggccctat tcataaagaa tcttctatgg
     2041 ctaacataag aataccacct ggtcatccag aaggctatct agaaggattc gcacaaattt
     2101 attctgatgc agctgatata attcaaaata ttggtaatgc taaagaactt ataaaaattc
     2161 tgccaaatat tgaagagggt ttacatatta tgaaatttat taat
//
LOCUS       DTVV01000244            1330 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1695584, whole genome shotgun sequence.
ACCESSION   DTVV01000244 DTVV01000000
VERSION     DTVV01000244.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1695584"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..816)
                     /locus_tag="EYO86_02915"
                     /pseudo
     CDS             complement(1..816)
                     /locus_tag="EYO86_02915"
                     /inference="COORDINATES: protein motif:HMM:PF16363.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            936..1319
                     /locus_tag="EYO86_02920"
     CDS             936..1319
                     /locus_tag="EYO86_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42020.1"
                     /translation="MNLQFEKQVEDKRGKILFLSLGTKKINIVEIKKGFARGGHFHNF
                     LSTHIIVYGKIELREENLKSNVEQIKKISAPSIINVQQNTAHLFIALEDSMFIEIFDG
                     EYQAADHPKYRKIIDEILHSSDNNC"
BASE COUNT          465 a          225 c          199 g          441 t
ORIGIN      
        1 acttaattta tctgctgaaa cattatagtg tctttcatcc tcaacttcag atttgatctg
       61 tatttcacat cctgtaattt tactaacctt ttctgcagct tgtagcaacg ttaaattttc
      121 tgaaataaca ttataaattc cagtcaaatt cttttctaat ccaaaaacaa ttgattctgc
      181 tgcatcgtct acatgtaaga aaggtctgtt ttgtttacct ccaaaaactg taatttttcc
      241 ttcttttatt gcctgagcag taaacagatt gataaccaaa tcaaatctca ttcttggtga
      301 gtaaccaaat aatgttccaa atcgtaatat caaaggtgat ctattaaccg aatgtgtggc
      361 cttttctgac aagtattttt gggtagcata gaaatccatg ggagaaactt tagatttttc
      421 tgtaatcaca atattaggct gacttccata aatagaacat gtgctggcaa aaacaaatgt
      481 ctctatttca tgaagctgac agagttcggc tatatttttt gtagctaaat aatttacttc
      541 ttcactagca ataggatcta tgcttgaagc aggcatacca acaatcgagg ctagatgaac
      601 tacagcatcc atgtcttgca ttgttgatac agtggtgact agatttcgaa tatcctcctg
      661 caccagttcg aaatctgcat tactggataa ttcttccagt gattcatttc cgtaccacaa
      721 tgcatctaaa actctgactt tatgtccttt agctaacatc tttctacaaa aaatcgagcc
      781 aagatatcct ccaccaccgg taacaagaat tttcattaaa taatcacttt aattgtaaat
      841 aacttatttc aatataaatg aaaaacatga aaattgatag tatagttttt agacaaagaa
      901 ttaatgaagc gattataaca aatatgtttt aaactatgaa tttacaattc gaaaaacaag
      961 tagaggataa acgggggaaa attttatttt tatcacttgg cactaaaaaa attaatattg
     1021 ttgagataaa aaaagggttt gctcgtggtg ggcactttca taattttcta tcaacccaca
     1081 ttattgtata tggaaaaata gaattaaggg aagaaaatct aaagagcaat gttgagcaaa
     1141 taaaaaaaat ttctgcaccc tcaataatca atgtacaaca aaatactgcg catcttttta
     1201 ttgctcttga ggattcaatg tttatcgaaa tatttgatgg tgaataccag gcggccgatc
     1261 atccaaaata tagaaaaatt attgatgaaa tactacattc aagcgataac aattgttagt
     1321 tgttagtaaa
//
LOCUS       DTVV01000245            1522 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1696154, whole genome shotgun sequence.
ACCESSION   DTVV01000245 DTVV01000000
VERSION     DTVV01000245.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1696154"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="EYO86_02925"
     CDS             <1..646
                     /locus_tag="EYO86_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HIC42021.1"
                     /translation="YISANTAASILLQGMTVEYLFERLYKIKKNEFLLFHAAAGGVGL
                     IASQWARSVGAKMIGTVSSDTKAELAEKNGCAFTINYKNENVFEKVKEITEGKGVTVV
                     YDGVGKDTFNVSLECLDFRGLFVSFGQSSGMVPIIDLHKTFNPKSLYYTRPTLMHYTS
                     TRNELEQSSNLLFNKIQNNEIKEHIFKTINLKDAAEAHTLIESRQSTGSIILKP"
     gene            complement(635..>1522)
                     /locus_tag="EYO86_02930"
     CDS             complement(635..>1522)
                     /locus_tag="EYO86_02930"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Cas7f/Csy3"
                     /protein_id="HIC42022.1"
                     /translation="SQELILDKGSSKKSKTLYHVANVAAIHSQKIGINHFPPLLMGSL
                     RVLFLFLFILPFFKFSIPKKNFKHLLLFSFIMGVCVYAFCYLALDFSSLVSPIIIGAQ
                     LTIPFGLILSKFILNENIPIKKWILIFSSFCGIVVVAYDPRFVEEIYGLFFIIIMAFF
                     YALANILSRYLKDINTVNQIGWHSFIGFIFLIIASTLIEGNPFNYLYPINYGGLLTAF
                     HAGIFVSLIGHGGLFYLYKYYPVATVLPYYSLFPLFGIALTFIIFLEIPGPYEIIGGI
                     IVIGSVYLIQLQDKQSLRL"
BASE COUNT          614 a          211 c          236 g          461 t
ORIGIN      
        1 ctatatctct gcaaatacag ctgcatctat tttactgcag ggtatgactg ttgaatatct
       61 atttgaacga ttgtacaaaa taaaaaaaaa tgaatttctg ctattccatg ctgcggctgg
      121 tggtgttggt ttaattgctt cacaatgggc tagatcagtt ggtgctaaaa tgattggaac
      181 tgttagcagt gatactaagg ctgaattagc tgaaaaaaat ggttgtgcat ttacaataaa
      241 ttataaaaat gaaaatgttt ttgaaaaggt caaagaaatt acagaaggaa aaggagttac
      301 agtagtctat gatggtgttg gtaaagatac ttttaatgtg tcattagaat gtttggattt
      361 cagaggttta tttgtttctt ttggtcaatc atcaggaatg gttcctatta ttgatttaca
      421 caagactttc aatcctaaaa gtctttacta cacaagacca acattaatgc actacacatc
      481 tacaagaaat gaattagagc aatcatcaaa tttactattt aataaaattc aaaataatga
      541 aattaaggaa catatattca aaactattaa tctcaaagat gctgctgaag cacacacttt
      601 gatagaatca cgacaatcaa ctggctctat tattttaaag ccttagagac tgtttgtctt
      661 gaagttgaat taaataaact gaacctataa caattatacc tccaattatt tcataggggc
      721 ctggtatttc taaaaaaata atgaaggtca gagcaatgcc aaataaagga aataaagaat
      781 aataaggtaa cacagtagca acgggataat atttatataa ataaaacaat ccaccatgtc
      841 caattaaaga aacaaaaata cctgcatgaa atgctgttaa taaaccacca taatttatag
      901 gatataaata attaaaagga tttccctcaa taagtgttga tgcaataatt aaaaaaataa
      961 atcctatgaa gctatgccaa cctatttgat taactgtatt aatatccttt aggtaacgag
     1021 acaaaatatt tgctaaggca taaaaaaaag ccattattat tataaaaaag agtccatata
     1081 tttcttcaac aaatcttgga tcataagcta caactacaat accacaaaaa gaagaaaaaa
     1141 ttagaatcca tttttttatg ggaatatttt cgtttaatat aaacttgctt aaaattaatc
     1201 caaaaggaat tgttaattga gctccaataa taataggaga aacaagagaa gaaaaatcta
     1261 atgcaagata acaaaaagca tatacacaaa ctcccattat aaaagaaaac aataaaagat
     1321 gcttgaaatt ttttttagga atagaaaatt taaaaaaagg aaggataaat aaaaataaaa
     1381 ataaaactct aagtgagccc attaatagag gcggaaaatg atttatgcca attttttgtg
     1441 agtgaatggc tgcaacattg gcaacgtggt aaagcgtttt gcttttttta ctgctgcctt
     1501 tatccagaat taattcctgc ga
//
LOCUS       DTVV01000246            1105 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1696347, whole genome shotgun sequence.
ACCESSION   DTVV01000246 DTVV01000000
VERSION     DTVV01000246.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1696347"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="EYO86_02935"
     CDS             complement(<1..553)
                     /locus_tag="EYO86_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC42023.1"
                     /translation="MDLINAIIVLLNFIFIPALSYGSQLALGALGVSFVYAILRFANF
                     SHGDLMSFGAMMTILFTWLFQSYGISLGFLPTALLAIPLAIIVTILFSIITDRFVFRH
                     YRIKKSTPVVFAMVSIGVMFVIQAIVRIIIGPDDRNFFDGQKFIIKAKQFKEITGLNE
                     GLAIKSTQVITIVVTIILVSLLFW"
     gene            complement(562..>1105)
                     /locus_tag="EYO86_02940"
     CDS             complement(562..>1105)
                     /locus_tag="EYO86_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC42024.1"
                     /translation="LSSGSVDFSGKVITNMLPQDRVSLGIAFVPQTQNVFTGMTVQEN
                     LEMGAFLRQDNITDTIDQIYELFPILKEKRNQSVGELSGGQRQQVAFGRALMTLPKIL
                     MLDEPTAGVSPIVMDDLFSRIIEVGKTGVGILMVEQNAKQALGIANRGYVMVNGENRI
                     EGTGQELLNNPDVRKSFLGG"
BASE COUNT          395 a          204 c          145 g          361 t
ORIGIN      
        1 accaaaataa taagctaacc aaaattattg taacaacaat tgtaattact tgagttgatt
       61 taattgctaa tccttcattt aatcctgtaa tttctttaaa ttgttttgct ttgataatga
      121 atttttgtcc atcaaaaaaa ttcctatcat ctggcccaat aataattcta actatagctt
      181 gtataacaaa cataactcca atggaaacca tcgcaaaaac tacaggagtg ctttttttta
      241 ttctgtaatg tcgaaaaaca aatctatcag taattataga aaataatatg gttacaataa
      301 ttgccaaagg gatagctaat aatgctgtag gcaaaaatcc aagagaaata ccgtaggatt
      361 gaaatagcca cgtaaaaaga attgtcatca ttgcgccaaa agacattaaa tctccatgtg
      421 aaaaatttgc aaaacgaaga attgcataaa caaaagatac accaagcgcg cctagagcaa
      481 gttgcgatcc ataagataga gctggaataa atataaaatt taataaaact ataattgcgt
      541 taattaaatc aatcacaata tttaacctcc aagaaatgat tttcttacat ctggattatt
      601 caataattct tgaccggtac cttctattct gttttcaccg ttaaccatta catatcctct
      661 atttgcaata ccaagtgctt gcttagcatt ttgttcaacc atcaaaattc caacacctgt
      721 tttgccaact tctattattc tggaaaaaag atcatccata acaataggtg atactccagc
      781 tgttggttca tctaacataa gtatttttgg caaagtcatt aatgctcttc caaaagccac
      841 ttgttgacgt tgacccccag ataactcacc aacgctttga tttctttttt cttttaatat
      901 tggaaacaac tcataaattt gatcgattgt atcagtaatg ttgtcttgcc ttaaaaatgc
      961 gcccatttct aaattttctt gaactgtcat tcctgtaaat acattttgag tttggggtac
     1021 aaaagcaatt ccaagactta ctctatcttg gggcaacata ttagttataa cttttccaga
     1081 aaaatcaaca gatccacttg ataaa
//
LOCUS       DTVV01000247            3397 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1707211, whole genome shotgun sequence.
ACCESSION   DTVV01000247 DTVV01000000
VERSION     DTVV01000247.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1707211"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="EYO86_02945"
     CDS             <1..1484
                     /locus_tag="EYO86_02945"
                     /inference="COORDINATES: protein motif:HMM:PF00929.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42025.1"
                     /translation="TTGKGKKASPNAFNQSPNWEQVLQVGAIVADDKFNQTNKSLDKR
                     ARLRTSIIPQPGAFLITQKTIKDALIAEHSSYELISIINQHFQEWKKDSPNTSFVGHN
                     SVEFDEPVLENNLFTNLFNPYITRPNRGDTLNLARGLYAINPSKINIHINEKGSQIFR
                     LELLAELNGLPVEMAHDALSDVRTTISLAKFLSENDPDVWKEISLTMNTEKGIEYIKK
                     NSGFCYVNYFFGKIKVQALSMVGESLYNGWFHAIDLQHDPKTLLESNNEEFKKLIKKK
                     DRWVIVNKNPIIFSGKFAKNYEPYKEIGTDLLNQRAKMVFKNKSLAERFKYMQIDRQL
                     EKEEQASQDNIFPEDKANAYLEFPKQDKDNINIFHSLKSWKEKYEVALKLNHPKASFI
                     VKRLIFDESPETLTDDDFRLVHRELHDRLVINQHRPFTTIPESMSYVDTEFSRIEDTG
                     ENKEKILMLKDFNKYLIFMEKYFSDKKAKPLKQGTELVKQIFG"
     gene            1537..2466
                     /locus_tag="EYO86_02950"
     CDS             1537..2466
                     /locus_tag="EYO86_02950"
                     /inference="COORDINATES: protein motif:HMM:PF00701.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIC42026.1"
                     /translation="MHKIHGTYCAALTPLNTDYSINKELFLKHCNSLLSEGLDGLAIF
                     GTTGEGNSFNVNEKIEAVNYLIDNNIGADKLIPGTGQCSIRDTVMYSKEVAKLKVKAI
                     LVLPAFYYKNVSNEGVIEFYKRVIEEVGDSDLKYILYNIPHVSGVSIGFEVIEQLIKL
                     FPNNVVGMKESSGDLDNMLKITKFFNEFSLFSGSDSLALKVCKRGGAGAITATSNISG
                     KLLSFIVNNHKEELSIDNFQELQLLQVKIRETLITHEPISALKALMSVIQDNQEWNRV
                     NPPLQRIENPQNHKTIIGLMQLLQKMDELVPSA"
     gene            complement(2449..2808)
                     /locus_tag="EYO86_02955"
     CDS             complement(2449..2808)
                     /locus_tag="EYO86_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIC42027.1"
                     /translation="MIFLLFIFIPIIEIAIFITVGSNIGILNTIAIILITALAGIYLV
                     RKQGLSLLFNAQRNLSQGVMPTEEIKGGIFLLISGLLLITPGFFTDCIGFAMFLKPVQ
                     NFVGLKARDYFQSRRRY"
     gene            complement(2824..3327)
                     /locus_tag="EYO86_02960"
                     /pseudo
     CDS             complement(2824..3327)
                     /locus_tag="EYO86_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT         1231 a          591 c          579 g          996 t
ORIGIN      
        1 aaacgacagg aaaaggtaaa aaagctagcc caaatgcatt taatcaaagt ccaaattggg
       61 aacaagttct gcaggttggg gccatagtag cagatgataa attcaatcag acaaacaagt
      121 ccttggataa acgtgcgcgt cttcgtacct caattatccc ccagccaggg gctttcttaa
      181 taacgcaaaa aacaataaag gacgcattga ttgctgaaca ctcatcttat gagttgatat
      241 ctataattaa tcaacatttt caggaatgga aaaaagattc tcccaatacg tcttttgttg
      301 ggcataactc tgttgaattt gatgagccag tattggagaa taatttgttc actaatttat
      361 tcaaccctta cattacaagg cccaacagag gagatacgct aaatcttgca aggggactgt
      421 atgcaatcaa tccatcaaaa ataaatattc acatcaatga aaaaggaagt caaatattca
      481 ggctagaact tttggctgaa ctaaatggac tgcccgttga gatggctcat gatgcgctaa
      541 gcgatgttag gacaacaatc tcactggcta aatttttatc tgaaaatgat ccagatgttt
      601 ggaaagaaat atcgctaaca atgaacacag aaaaaggcat cgagtacata aaaaaaaaca
      661 gcggtttttg ctatgttaat tatttttttg gaaaaattaa agtgcaggcc ttgtccatgg
      721 ttggggagtc tctttacaat ggatggtttc acgccataga tttgcaacat gatcccaaga
      781 cacttcttga atcaaacaac gaagaattca aaaaattaat caagaaaaaa gacaggtggg
      841 ttatcgttaa caaaaatcct ataatatttt caggcaagtt tgcaaaaaat tatgaaccct
      901 acaaagaaat tggtacggac ctactaaatc aaagagctaa aatggttttt aagaataaat
      961 cacttgccga aagattcaaa tatatgcaga tcgatcggca gcttgaaaaa gaagaacagg
     1021 cctcacagga taatattttt cctgaagata aagccaatgc atacttggaa tttccaaaac
     1081 aagacaaaga caacataaat atattccaca gccttaaatc atggaaggaa aagtatgagg
     1141 tggcccttaa actaaaccac cctaaagcaa gctttattgt taaacgttta atttttgatg
     1201 aaagcccaga gacattgact gatgatgatt ttagattagt gcaccgtgaa ttgcatgacc
     1261 gattggttat caatcagcac agaccattta caaccatacc agagtccatg agctatgtgg
     1321 acactgaatt tagcagaatt gaagatacag gcgagaataa agaaaaaatt ttaatgctta
     1381 aagattttaa taaataccta atattcatgg aaaaatattt ttctgacaaa aaagcaaaac
     1441 ctctaaaaca aggaacagaa ctcgtaaaac aaatttttgg ttaatattaa gttacaatat
     1501 gtagaatttc tttaagtatt actttattag cagtttatgc ataaaataca cggcacttat
     1561 tgtgcagctc taacccctct caatactgat tattctatta ataaagaatt gtttcttaag
     1621 cactgtaaca gtttactgtc agaaggtctt gatggccttg caatatttgg aactacagga
     1681 gagggaaatt cttttaatgt gaatgaaaaa atagaggctg ttaattattt aatcgataat
     1741 aatattggtg cagacaagct tattcccggt acgggtcagt gctctatcag agatacagtt
     1801 atgtactcta aagaggttgc aaaattaaaa gtaaaagcaa tattagtttt acctgcgttt
     1861 tattacaaga atgtttctaa tgaaggtgtt atagaatttt ataaacgagt tattgaggaa
     1921 gtaggagaca gtgatctaaa atatattcta tataatatac ctcacgtatc aggagtttca
     1981 attggctttg aagttattga acaattaatt aaactgtttc ctaataatgt agtgggaatg
     2041 aaagaatcaa gtggtgatct agataatatg ctgaaaataa caaaattttt taatgaattc
     2101 tctctttttt caggctctga tagtttggct ttaaaagtgt gcaagagggg cggagcaggg
     2161 gctatcaccg ctacttccaa catttcggga aaactactta gttttattgt gaataatcac
     2221 aaagaagaat tatccattga taattttcaa gaactgcaat tacttcaagt aaaaataaga
     2281 gaaaccctca ttacgcatga gcctattagt gccttaaaag cccttatgtc tgtaatacaa
     2341 gacaaccagg aatggaatag agttaaccct ccactgcaga gaatagaaaa tcctcagaat
     2401 cataaaacta taattggatt aatgcaatta ctgcagaaga tggatgaatt agtaccgtct
     2461 gcgtgattga aaataatctc tcgcttttaa tcccacaaaa ttttgaacag gttttaaaaa
     2521 catggcaaag ccaatgcagt cggtaaaaaa accaggcgta attagaagta agcctgaaat
     2581 aagtagaaaa attccaccct taatttcttc tgttggcata acaccttgag agagattgcg
     2641 ttgtgcattg aataaaagac taagaccttg ttttctaact agataaattc cagctaaagc
     2701 cgttatcaat attatggcga tggtattcaa tataccaatg tttgatccca cggtaataaa
     2761 aatagcaatt tcaattatag ggataaaaat aaaaagtaaa aatatcattt ttaaataaat
     2821 atattatctt tccaaagcct cgatctctct aattttaatt tgcttttcaa attcccttag
     2881 acgtctatat acacttgcca actcaataat agttggccaa gacttaaaca agtattgaag
     2941 tgatccctct accctaccaa aagctcttag tatttgctgc attacgccaa gagtcattac
     3001 acctgcaaca atggcaggtg ctaataaaac atatccaacc aacacattcg cctgcagata
     3061 ggccaatcta cctatattaa aataaagata tcgcagataa cttttgtaat taatttttct
     3121 tacaccatca aataactctt ccagcgtttt tggccttacg gttccgtcgt cttccgctat
     3181 aacaagtatt ttgcgatacg cggcttctct cttttgtaag tcgtattcaa tacctacgag
     3241 ccttaacaag taagccaata taatcattaa tatagtgccg cctactgccc aaagaaaagc
     3301 acctgctact aaagaatatt cccaatcctg aaacgattgg cgcaaagtaa gtaataattt
     3361 gttttctgtg ttgactatct cttactgata tatcaac
//
LOCUS       DTVV01000248            1228 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1715984, whole genome shotgun sequence.
ACCESSION   DTVV01000248 DTVV01000000
VERSION     DTVV01000248.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1715984"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="EYO86_02965"
     CDS             complement(<1..485)
                     /locus_tag="EYO86_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIC42028.1"
                     /translation="MTKIAPSILAADLSKINNEVINVDKAGAEYIHIDVMDGRYVPNT
                     SFGPNIVETLRPLTKKILDVHLMITPVMPYIKAFIDAGADIISFHPEADDNSKTIIET
                     IKSANCKAGIAIHPNVEIEAIQEFLNDIDLVVVMTVVPGFGGQKFLDTQLSKINKLYN
                     IR"
     gene            536..880
                     /locus_tag="EYO86_02970"
     CDS             536..880
                     /locus_tag="EYO86_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIC42029.1"
                     /translation="MTSIVSKPWGSFQVIEEGEKYMVKKIIVKPKGKLSLQSHEHRSE
                     HWVVVKGRAQVTLNDDIKILETNKSIFIPLQAKHSLANNELEDLVVIEVWHGNILEEE
                     DIKRYEDIYGRE"
     gene            882..>1228
                     /locus_tag="EYO86_02975"
     CDS             882..>1228
                     /locus_tag="EYO86_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42030.1"
                     /translation="MIVNAPISVGELMDKISILKIKKKNITDEKKLLFINEELQLLSS
                     TLNAVVQDNKINEFLDKLIEVNSKLWKIEDDIRLCERNKKFNQHFIDLAREVYITNDK
                     RTDIKLAINNHFGS"
BASE COUNT          472 a          176 c          202 g          378 t
ORIGIN      
        1 cgaatattat ataatttgtt aattttggac agctgtgtgt ctaaaaattt ttgtccacca
       61 aaacctggaa caaccgtcat tacaaccaca agatcaatat catttaaaaa ttcttgtatt
      121 gcctcaattt ctacatttgg atgaattgca atcccagcct tgcaattagc acttttaata
      181 gtttctatta ttgttttcga attatcatca gcctcggggt gaaagctaat tatatccgct
      241 ccagcatcaa taaaagcttt aatataaggc attactggag tgatcatcaa atgtacatcc
      301 aatatttttt tagttaaagg gcgtaaggtt tctactatat tgggaccgaa agaagtattg
      361 ggaacataac ggccatccat aacatcgata tgaatatatt ctgcaccagc cttatctacg
      421 ttgataactt cattgttaat ttttgacaaa tcagcagcaa gaatggaggg agcaattttg
      481 gtcataaatt aatagttaaa gaaataaaat aataatatat accgattcca aaacaatgac
      541 atcaatagtt tcaaaaccat ggggctcctt tcaagtaatt gaagagggag aaaaatatat
      601 ggttaaaaag attatagtta agccaaaagg taaattatca ttacaaagtc atgaacatcg
      661 ttctgagcat tgggttgttg ttaaagggag agctcaagta acactaaatg atgatataaa
      721 aatacttgaa acaaataaaa gtatatttat tcctcttcag gcaaaacaca gtcttgcaaa
      781 taatgaattg gaagatttgg ttgttattga ggtatggcat ggaaacattc ttgaagaaga
      841 agatataaaa cgttacgaag atatctatgg cagagaataa gataatagtt aacgctccta
      901 tttctgttgg tgaattaatg gataaaattt ctatcttaaa aatcaaaaag aaaaatatta
      961 cagatgaaaa gaaactactt tttattaatg aagagctgca actactatca tctacactga
     1021 atgcagttgt tcaggataat aaaatcaatg agtttttaga taaattaatc gaagtaaact
     1081 caaaattgtg gaaaatcgaa gatgatattc gactttgtga aagaaataaa aaatttaacc
     1141 agcattttat cgaccttgca agagaggtat acattacaaa cgacaaaaga acagatatca
     1201 aattagcaat taataatcat tttggttc
//
LOCUS       DTVV01000249            1746 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1717040, whole genome shotgun sequence.
ACCESSION   DTVV01000249 DTVV01000000
VERSION     DTVV01000249.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1717040"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="EYO86_02980"
     CDS             complement(<1..541)
                     /locus_tag="EYO86_02980"
                     /inference="COORDINATES: protein motif:HMM:PF03807.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIC42031.1"
                     /translation="MKKIGILGGGVWGSALAKLLSNNKVFIYARDKNTVQSINDHKFN
                     PKLKYTVFNENVTSTENTKTFKDLEYLFIALPSQTIREVLTNLSLTNKDQEIIVASKG
                     IEISTSDLLSEVVNNTAKSKKISILSGPCFSDEVAQNLPTAVTLASNNKETFRRVNSL
                     FQNKNFRLYYSDDLIGCQIG"
     gene            complement(543..1658)
                     /locus_tag="EYO86_02985"
     CDS             complement(543..1658)
                     /locus_tag="EYO86_02985"
                     /inference="COORDINATES: protein motif:HMM:PF01266.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIC42032.1"
                     /translation="MTDFSVETVVIGAGVVGLSISRELSNINGEILVLESEKDFGQLI
                     SSRNSGVIHAGIYYPENSLKSKFCIEGNTLLYEYCKKYNIPYRNTKKILVASSLEQVQ
                     IIDQIKINAEKNGVRNIKKLTKSQTLQLEPLIRCEESLLVPSSGIVDAVSFMRSLEGQ
                     ILDSGNMISYQSLVSKINFDGKIFTLRVLDNNNQETTIKCRNLINSTGLYATDIANKI
                     EGLKKEFVPKTFFAKGNYFSINKDLGIRHLVYPIPNKFSLGIHLTPELDYSVKFGPDF
                     EWVEDRENYKVDINKKKLFINEIKKYLPDFDSNSLNPNYAGIRPIIEKKEKSKRDFII
                     QTDSIHSIPNLINLYGIESPGLTSSLAIALHVRDLLE"
BASE COUNT          566 a          290 c          226 g          664 t
ORIGIN      
        1 cccctatttg acacccaata agatcgtctg aataatataa tctaaaattc ttattctgaa
       61 ataatgaatt aacccttcta aaagtttcct tattgttgga ggctaatgtt actgcagttg
      121 gtagattttg tgctacttca tcagaaaaac atggtccaga taatatacta atttttttac
      181 ttttagctgt attattgaca acttcactta ataaatcgga tgtagaaatt tctattccct
      241 tggaggctac tattatttct tggtctttat tagttaagga taaatttgtt aatacttccc
      301 ttatggtctg agatggtaaa gcaataaata aatattctag atccttaaat gttttagtat
      361 tttcagtcga agttacattt tcattaaata ctgtatattt taatttggga ttgaacttat
      421 gatcattaat agattgaacc gtgtttttat cacgagcata aataaaaact ttattattgc
      481 ttaacaattt agcaagagca cttccccaaa cgcccccacc taatattcca atttttttca
      541 tactactcca ataaatctct tacatgtagc gcaatcgcta atgaagatgt taatcctggt
      601 gactcaatac catacaagtt aattaaattg ggaattgagt gaatactatc tgtttgaatt
      661 ataaaatctc tcttcgattt ttctttcttt tctataatag ggcgaattcc agcataattt
      721 ggattaagtg aattagaatc aaaatctggg agatattttt ttatttcatt aataaataat
      781 ttttttttat ttatatcaac tttgtagttt tctctatcct caacccattc aaaatcagga
      841 ccgaacttaa ctgaataatc caattccgga gtcaaatgta ttcctagtga aaatttattt
      901 ggtataggat aaacaagatg acgaattcct aaatccttat tgatactaaa ataatttcct
      961 ttagcaaaga aagttttggg tacaaattct ttttttaacc cctctatttt atttgcaata
     1021 tctgttgcat ataaacctgt tgagttaatt agatttctac acttaatagt tgtttcttga
     1081 ttattattgt caagaacacg taatgtaaat attttaccat caaaatttat tttacttact
     1141 aaggattggt atgaaatcat attacctgaa tccaagattt gaccttccaa tgaacgcata
     1201 aaacttactg catccacaat gccactagac ggaaccagca gagattcttc acaccgtatt
     1261 aaaggttcta attgcaatgt ttgtgactta gttaattttt taatatttct gactccattt
     1321 ttttcagcgt taatttttat ttgatcaata atctgaactt gctctaaact tgaagcgaca
     1381 agaatttttt ttgtattgcg ataaggaata ttatattttt tacaatactc atacaaaagg
     1441 gtgtttcctt cgatacaaaa ttttgatttt aatgagtttt ctggataata aattcctgca
     1501 tgaataaccc ctgaatttct agagctaata agttgtccaa aatctttctc tgactctaaa
     1561 acaagtattt ctccattgat gttcgacaac tctctggata tggacaatcc cactacacca
     1621 gccccaatta ccaccgtttc aacgctaaaa tctgtcattt aaaccatatt tagactattt
     1681 ttttaaaaaa actaagtatt gatctctgta cctaataatt gttttagatt ataggtgcct
     1741 tataga
//
LOCUS       DTVV01000250            1053 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1717492, whole genome shotgun sequence.
ACCESSION   DTVV01000250 DTVV01000000
VERSION     DTVV01000250.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1717492"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            2..>1053
                     /locus_tag="EYO86_02990"
     CDS             2..>1053
                     /locus_tag="EYO86_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HIC42033.1"
                     /translation="MENKKIKELIDLAISYIPKSEVKVISKAITFSSQAHDKQLRKSG
                     EPYINHPIEVAKILTGIKLDVSSIVTGLLHDTVEDTIITTSEIKNIFGNEVSNLVDGL
                     TKINKFSLKVKNLKLGENYRKLLLAATKDLRVILIKLADRLHNMQTLRFVDNHHKRIS
                     TSLETLDIYAPLAQRLGMREWQDKLEDLSFQFINPEARNSILERLEYLNSKDVNVVDE
                     IRYELNKSFIDENVDCKIYGRIKSPYSIWNKIKNKDISFEQLSDIMAFRIITNSTREC
                     YKLLGVTHRKYSYIHGRFKDFISSPKINGYRALHTSVIGPQNRKIEIQFRSNVMNQIA
                     EFGVAAHWKYKDPKEI"
BASE COUNT          416 a          126 c          170 g          341 t
ORIGIN      
        1 tatggaaaac aaaaaaataa aagaattaat tgatcttgct atttcgtata ttccaaaatc
       61 tgaagttaaa gtaatctcta aggccattac ttttagcagt caagctcatg acaagcagtt
      121 aagaaaatct ggagaaccat atattaatca tccgattgaa gtagccaaaa ttctcactgg
      181 aataaaacta gacgtctcat ccattgttac tggtttactt catgatacag ttgaggatac
      241 aataataact acaagtgaga taaaaaatat ttttgggaat gaagtatcta atttagtaga
      301 tggcttaact aaaattaaca agttttcgtt aaaagtaaaa aacttgaaac ttggagaaaa
      361 ctatagaaaa ttactattag cagcaacaaa ggatttgagg gtaattttaa ttaaattggc
      421 tgatagactt cataacatgc aaactttaag gtttgttgat aatcatcata aaagaattag
      481 cacttcttta gaaacactag atatttatgc gccgttggca caaagattag gaatgagaga
      541 atggcaagac aaattagaag atttatcttt tcagtttatt aatcctgaag ctaggaattc
      601 aattttggaa agattagaat atttaaattc taaggatgtc aatgttgtgg atgagataag
      661 atatgaatta aataaatcat ttatagatga gaatgttgat tgtaaaattt atgggagaat
      721 aaaatcacct tattctattt ggaataagat aaaaaataaa gatatttctt ttgaacaatt
      781 gtcagatatc atggctttta ggattataac taattctact agagaatgtt ataaattatt
      841 aggtgtcacg catcgtaaat attcttatat ccatggtcgc tttaaagatt ttatatcatc
      901 tcctaaaatt aatggttatc gagctttaca tacatcagtc ataggaccac aaaatagaaa
      961 aattgaaatt caatttagat caaatgtaat gaatcaaatt gcagaatttg gtgttgctgc
     1021 tcattggaaa tataaagatc caaaagaaat taa
//
LOCUS       DTVV01000251            1064 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1718317, whole genome shotgun sequence.
ACCESSION   DTVV01000251 DTVV01000000
VERSION     DTVV01000251.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1718317"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            64..1064
                     /locus_tag="EYO86_02995"
                     /pseudo
     CDS             64..1064
                     /locus_tag="EYO86_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
BASE COUNT          377 a          181 c          177 g          329 t
ORIGIN      
        1 gataaatcaa tccattttcg tacatccgca caaaagtttc attcacaccc cgattgagct
       61 gcggatccat cgtatacttc atctgagttt gatttttatc ttgaagattt aagtcccaaa
      121 gcacttatag taaaagaagg ttcaaattct ccggtaattg aagtagcaaa aaaaagagag
      181 attaaaatat ttaatctatt agttagtact aaagatcctt caggaaaatt tagtttagca
      241 tccaaagaag aaaatatatc taatgctata aatcaaaatg ataatattgt tccagatgac
      301 atagctttaa ttcttcatac ctcaggaaca acttcaagac caaaaatggt tcctttaact
      361 catttaaatc tttgtacttc tgcaaaaaat atcgttcaaa ctctaaattt aaatagatcc
      421 gataaatgta taaatatcat gcctttattt catattcatg gaattgttgg tttattgctt
      481 tcttcgttat tttctggagg aactattttt acttcacctg gatttaacgc attaaaattt
      541 ttttcgtggc ttaaaacatt ctctccaact tggtatagcg ctgttccaac aatgcaccag
      601 gtaattctca cccgagctag tagaaattct gaaattattg ctgagacaaa attgagattc
      661 attagatcta gctccgcacc cttaccttcg acaacaatga aagaaataga aaatactttt
      721 cattgcccag ttatagaatc gtatggaatg accgaggccg ctcatcaaat gacgagcaat
      781 catttacctc ctggtaaaag aaaagccaca aaagctggtt ttgcagccgg tccagaaatt
      841 gcaattatgg gaaacaaaaa aaatatttta gaaaagggca agattggaga aattgtcatt
      901 aggggagaaa gtgtcacaaa aggatactta aataacccta aagctaataa tgattccttt
      961 atagaaggat ggtttcgaac aggggatcag ggtttttttg atgaagatga tttactccaa
     1021 ctaactggtc gcattaagga aattattaat aaagggggtg aaaa
//
LOCUS       DTVV01000252            1127 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1720819, whole genome shotgun sequence.
ACCESSION   DTVV01000252 DTVV01000000
VERSION     DTVV01000252.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1720819"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="EYO86_03000"
     CDS             <1..903
                     /locus_tag="EYO86_03000"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017969397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIC42034.1"
                     /translation="SVEDLSIPEVEILLNKANFYADANRQKGKIEKTLKGSTIITLFF
                     ETSTRTKTSFELAAKRLGADSIGINASASAIKKGETLIDTAMTLNAMHADILIVRHPD
                     SGAVKLLSKKVNCAVINAGDGMHEHPTQALLDALTIKRNKNKIKGLNIALCGDVLHSR
                     VARSNILLLSKLGARIRIIAPPTLIPKGIENLGVEIFNNMEKGLVNCDIVMMLRLQTE
                     RMKGLYVPSTREYFKFYGLDHKKLKNAKKDALIMHPGPMNRGVEIDSSLADNLDRSVI
                     HQQVEIGVAVRMACLEMLTVGIDK"
     gene            900..>1127
                     /locus_tag="EYO86_03005"
     CDS             900..>1127
                     /locus_tag="EYO86_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIC42035.1"
                     /translation="MKDAYINAKILDPEKNKEFIGDIIVEKDKIIDIGPNVYKKDKPK
                     SYTNVHDCKNLFISPGLLDMRVSLGEPGSEHK"
BASE COUNT          466 a          135 c          205 g          321 t
ORIGIN      
        1 agtgttgaag atttatctat tcctgaagta gaaattttac taaataaagc taacttttat
       61 gcagatgcaa ataggcaaaa aggtaaaata gaaaaaacat taaaaggatc aactataatt
      121 accttattct ttgaaacttc tacaagaaca aaaacatcgt ttgaacttgc tgcaaaaaga
      181 ttaggtgcag atagtatagg tattaacgca tctgcaagtg caataaaaaa aggtgaaaca
      241 ttaattgata cagcaatgac attaaacgca atgcatgcag atattcttat agtaagacat
      301 ccggatagtg gtgctgtaaa attactatct aaaaaagtta attgtgcagt aataaatgca
      361 ggagatggca tgcacgagca tccaactcaa gctttacttg atgcgttaac aataaaaaga
      421 aataaaaata aaattaaagg acttaacatt gcattatgcg gagatgtttt acacagtaga
      481 gtagcaagat ctaatatttt acttctctca aaattaggtg caagaataag aattattgcc
      541 ccaccaactt taatcccaaa aggtatagaa aatttaggtg ttgaaatatt taataacatg
      601 gaaaaaggtt tggtaaattg tgatatcgta atgatgctaa ggcttcaaac agaaagaatg
      661 aaaggattgt atgtaccatc aacaagagaa tattttaaat tctatggttt agatcataaa
      721 aaattaaaaa atgcaaaaaa agatgcgtta attatgcatc cagggcctat gaatagagga
      781 gtagaaattg atagttcgct agccgataat ttagatagaa gtgtaataca tcaacaagta
      841 gaaattggtg ttgctgtaag aatggcatgt ttagaaatgt taactgtggg tatagataaa
      901 tgaaagatgc atatataaat gcaaaaatat tagatccaga aaaaaataaa gaatttattg
      961 gtgatattat tgtagaaaaa gacaaaatta ttgatattgg tcctaacgtt tataaaaaag
     1021 ataaaccgaa aagttataca aatgttcatg attgtaagaa tttgtttata agcccaggac
     1081 tcttagacat gagagtttcg ttaggagaac ctgggtcaga acacaaa
//
LOCUS       DTVV01000253            1609 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1721859, whole genome shotgun sequence.
ACCESSION   DTVV01000253 DTVV01000000
VERSION     DTVV01000253.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1721859"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            114..1055
                     /locus_tag="EYO86_03010"
     CDS             114..1055
                     /locus_tag="EYO86_03010"
                     /inference="COORDINATES: protein motif:HMM:PF02668.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42036.1"
                     /translation="MLTGKILDEILTDDNLSWGRNLENNKQKFLVELNSEIIEELIKS
                     RNELDKIDENELPILKNNILKIRKEKLLDGVGLFIIDGVCLKNFSINEKKSIFILISK
                     ILGELLIQNIKQEKIVVIKDLGKSMKTGGRYHETKEGGSHHTDSPQLKNVPDWLGLFC
                     VHNAKKGGTNLFLSAYAIHNIIFKERKDLLNLLYEKFHFDKRGEFKDGESPTVFEPIF
                     EYKEGRLRFRYLRNYINAGHDIQNQPLSKSQKEVLDFLDNLMRDENIILRYDLKPGDI
                     VFSDNHWILHGRTTFEDYDDEKLKRLMLRTWVKDRMS"
     gene            1174..>1609
                     /locus_tag="EYO86_03015"
     CDS             1174..>1609
                     /locus_tag="EYO86_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42037.1"
                     /translation="MNELEKKSKRIHPFLVAFFPILIIYSQNVGRIEIEELVLPTIVI
                     VGPAIGLYYFLKSILKNENKSAIIVTLILVILFSYGHIYYLLNDVMIDEFDIGRNRYL
                     IPVFGLSLGIGIFFTIKIKTALDNATTILNVISVTLILVAAGN"
BASE COUNT          633 a          166 c          272 g          538 t
ORIGIN      
        1 ggtaaagaaa tcatacaaaa aaaattcaac tgggataata tagctaaaaa atttgtgtta
       61 attttaaatc aaaatattca gtgaaattaa ttaaacggta ataatatgca gttatattga
      121 ctggaaaaat acttgatgaa atattaactg acgataatct gagttggggt cgaaacctag
      181 aaaataataa acaaaaattc cttgtagaac tcaattcgga aattattgaa gagctaatta
      241 aaagtaggaa tgaattagat aagattgatg aaaatgagtt accgatttta aagaataata
      301 tactaaaaat cagaaaagaa aagttgctag acggagtggg tttgtttata atagatggtg
      361 tttgtttgaa aaatttttca attaatgaaa aaaaatccat ctttatactc atatctaaaa
      421 ttttaggtga attattaatt caaaatataa aacaagaaaa aattgttgtg ataaaagatt
      481 taggaaaatc aatgaaaact ggtggcagat atcacgagac gaaagaagga ggatctcacc
      541 acactgatag cccacaattg aaaaatgttc ctgattggtt aggtcttttt tgtgttcata
      601 atgcaaaaaa aggtggtaca aatttgtttt tgagtgctta tgctattcat aatataattt
      661 tcaaagaaag aaaagatttg ctaaatttat tgtatgaaaa atttcacttt gataaaagag
      721 gagaattcaa ggacggtgaa tcacccacag tttttgaacc gatttttgag tataaggagg
      781 gaagattaag attcagatat cttcgaaatt atattaatgc tggtcatgat attcaaaatc
      841 agcccttgtc taaatctcaa aaagaggttt tagattttct tgataattta atgcgtgacg
      901 aaaatatcat acttagatat gatttaaagc ctggagatat agttttttca gataaccact
      961 ggattcttca tggtagaaca acctttgaag actatgatga tgaaaaatta aagagactaa
     1021 tgttaagaac ctgggttaag gatagaatgt cgtaaaataa aatttatctt tgattaaaat
     1081 ttatggaatc aggttttcca tgggtagaaa aatagtttaa attaatttct tagaaagagg
     1141 ttaatatcat ctattttaaa aaatgaggca gttatgaacg aattagagaa aaaatcaaaa
     1201 agaattcatc catttttagt tgcatttttt ccaattttaa tcatttattc acaaaatgtg
     1261 ggaaggattg aaattgaaga attagttctc ccaacaattg taattgttgg acctgcaata
     1321 gggctttatt attttttgaa atcaatttta aaaaacgaaa ataaatctgc aataatagta
     1381 acactaattc ttgtaatatt attttcatat ggtcacattt actatttgtt aaacgatgtt
     1441 atgatagatg agtttgatat aggtaggaat agatatctaa ttccagtatt tggattatca
     1501 ttaggtattg gtatattttt tacaattaaa ataaaaacag cattagacaa tgcaacaact
     1561 attttgaatg taatttcggt gactttaatt ttagttgcgg caggaaata
//
LOCUS       DTVV01000254            1112 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1722757, whole genome shotgun sequence.
ACCESSION   DTVV01000254 DTVV01000000
VERSION     DTVV01000254.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1722757"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="EYO86_03020"
     CDS             <1..264
                     /locus_tag="EYO86_03020"
                     /inference="COORDINATES: protein motif:HMM:PF07021.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIC42038.1"
                     /translation="PYVWYETPNIHFFTIKDFQDLCKQSNIIIEKSIALTSKGRQFEI
                     SPGISGANLFTSEAIFLLSYQNFAPIKLQSKQKTFVSNSALVN"
     gene            264..1031
                     /gene="gloB"
                     /locus_tag="EYO86_03025"
     CDS             264..1031
                     /gene="gloB"
                     /locus_tag="EYO86_03025"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIC42039.1"
                     /translation="MTDPQIKVKIIPQLKDNYSYVIYSDEKKLAAIIDPAESTSIIDF
                     IQKKSLTIETIILTHHHDDHTAGVQDILNFSSVSVYSPDKKISRTSQVVRNNDQINLG
                     FLSLKVFATPGHTLDHLIFYDEHNKILFSGDTLFRFGCGRVFEGTYEQMQLSLQLLVG
                     LDNAAQVYCGHEYTLKNLEFLLSIFPDNEELKISKQRINEQINTTGSSIPFNLGEEKR
                     LNPFLNPRSSYYQDFMKKNKFNDLAMFSYLRDLKNKY"
BASE COUNT          408 a          182 c          149 g          373 t
ORIGIN      
        1 ccttatgtgt ggtatgaaac gcccaatatt cattttttta caataaaaga ctttcaagat
       61 ctctgtaaac aatccaatat tatcattgaa aaaagtatag ccttaacaag taaaggaagg
      121 caatttgaaa tatcaccagg tatatcaggt gcaaatttat ttacaagtga agcaatattt
      181 ttattaagct atcaaaattt tgcgcccatc aagttgcaat caaaacagaa aacatttgtt
      241 agcaattcag ctctagttaa ttaatgaccg acccacaaat aaaagttaaa attattcctc
      301 aacttaaaga caattattcg tatgttatat attcagatga aaagaaattg gcagctatta
      361 ttgatcccgc tgaatctaca tctataattg attttatcca aaaaaaaagt ttaacaattg
      421 aaactattat cttaacacat catcatgatg atcatacggc aggtgttcaa gacatactaa
      481 attttagtag tgtatctgtt tatagtccag ataaaaaaat ttcgagaaca tcccaagttg
      541 taagaaataa tgaccaaatc aatcttggtt tcctttcttt gaaggtattc gctactccgg
      601 gtcacaccct tgatcatctt atcttttatg atgaacataa taaaattctt ttctccggtg
      661 acacgctttt tcgctttggt tgtggaagag tttttgaagg tacatatgaa caaatgcaat
      721 tatctcttca actacttgta ggattagata atgcagcaca agtttattgt ggtcacgaat
      781 atactctaaa aaatctggaa tttttactat ccatctttcc cgacaatgaa gaattaaaaa
      841 ttagcaaaca aagaattaat gaacagatta atactacagg aagctccatt ccctttaatt
      901 taggagaaga aaaacgactt aatccctttt taaatccaag atcttcttat taccaagatt
      961 tcatgaaaaa aaataaattt aacgatttag caatgttctc atatttaaga gatttaaaaa
     1021 ataaatatta accaattcaa ccagatttta gtgaatttag ctgttaattt tgtttgccta
     1081 ttctgattga acattataaa tcccagtttt tc
//
LOCUS       DTVV01000255            1296 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1729913, whole genome shotgun sequence.
ACCESSION   DTVV01000255 DTVV01000000
VERSION     DTVV01000255.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1729913"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="EYO86_03030"
     CDS             <1..910
                     /locus_tag="EYO86_03030"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13602.4,HMM:PF16884.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIC42040.1"
                     /translation="DNDDDVMIQNQWVSVDPYMRARMTERKNYKPPFEIGKPMEGSCI
                     GHVIDSKSKELQKGDIVLSEYGWRDKFVTNYINLKKINPINVPNQTYLGPLGFTGHTA
                     YIGLFRIGKLKKNQTVLVSSAGGSVGSTVCQIAKIMGCKVIASTGNDEKVEWLKNELS
                     VDHAFNYNKVDNLVLHLKSLSPEGYDLYFDNVGGEFLEAAIFRMKNYGRIVICGRISQ
                     MNATSPGEGLKNMAHVLVKRLTIKGFLIFDHGDDYEDFENDMKQWITNKQMKWRETVV
                     EGLENAPQAFIDLLDGKNIGKMIVKI"
     gene            complement(907..>1296)
                     /locus_tag="EYO86_03035"
     CDS             complement(907..>1296)
                     /locus_tag="EYO86_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2083 domain-containing protein"
                     /protein_id="HIC42041.1"
                     /translation="IHIPRHGGSCPRWNVYIAFLNPGRIHPQISKMPDGKTYFCIARA
                     FEKGIEKHGMPKSFVSIGLGCDIQYAKELTYSEGMDLQNKKLETPIGVSCRICPREDC
                     QQRAFPPIDKELKLDISYRGTSPYVTI"
BASE COUNT          445 a          193 c          244 g          414 t
ORIGIN      
        1 agataatgat gatgatgtta tgatccaaaa tcaatgggtt tcagttgatc cctacatgag
       61 ggcacgaatg actgaaagaa aaaattataa acctcctttt gaaataggca aaccaatgga
      121 aggctcatgc ataggacatg ttattgatag caaatccaaa gaattgcaaa agggcgatat
      181 cgttttaagt gagtatggat ggagagataa atttgtaaca aattacatca acttaaaaaa
      241 aattaaccca attaatgttc caaatcaaac ataccttgga ccactcggct ttacgggtca
      301 tactgcatat attggtttat ttagaatagg taagctgaaa aaaaatcaaa ccgtgctcgt
      361 atcttctgct gggggtagcg ttggttcaac tgtttgccaa attgcgaaaa tcatgggttg
      421 caaagttatt gcaagcacag gcaatgacga aaaggttgaa tggttaaaaa atgaattgag
      481 cgttgatcat gcttttaatt ataacaaagt tgataatctg gttttacatc taaaatcttt
      541 atctcctgaa ggatatgatc tctattttga caatgttggg ggtgagtttt tagaagctgc
      601 tatctttaga atgaaaaatt atggtagaat agttatttgt ggtcgtatat cacaaatgaa
      661 tgcaacttct cctggtgaag gcttaaaaaa tatggctcat gtattggtga aacgtttaac
      721 aatcaaaggc tttttaatat ttgatcatgg tgatgattat gaagattttg aaaatgatat
      781 gaaacaatgg attacaaata aacaaatgaa atggagggaa acagttgtag agggattgga
      841 aaatgcacct caagcattca ttgatctgct tgatggaaaa aatattggca aaatgatagt
      901 taaaatttaa atcgttacgt aaggtgaagt tcctctgtaa ctaatatcta gttttaattc
      961 cttatcaatt ggtggaaaag ctctttgctg acaatcctct cttggacaaa ttctacaaga
     1021 aactccaatc ggtgtttcta attttttatt ttgtaaatcc ataccttcag aataagttaa
     1081 ttccttcgca tattgtatat cgcagcccaa tcctatcgaa acaaaacttt ttggcatacc
     1141 gtgtttttca attccttttt caaaagctct ggcaatacaa aaatacgttt tgccatctgg
     1201 catttttgat atttgtggat gtattctacc aggatttaaa aaagctatat agacattcca
     1261 tctaggacaa gatcctccat gacgaggtat atgaat
//
LOCUS       DTVV01000256            1142 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1732142, whole genome shotgun sequence.
ACCESSION   DTVV01000256 DTVV01000000
VERSION     DTVV01000256.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1732142"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="EYO86_03040"
     CDS             complement(<1..168)
                     /locus_tag="EYO86_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HIC42042.1"
                     /translation="MKDRESKSFAKQIKRYAKVGGIVGALATKLAGQKYLGVKLDKRK
                     HAAEVRNALGNI"
     gene            complement(178..>1142)
                     /locus_tag="EYO86_03045"
     CDS             complement(178..>1142)
                     /locus_tag="EYO86_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIC42043.1"
                     /translation="YSNLSHRYYAMKAKWFGVKSLKYWDRNAPLPFQSKKQFTWNKAK
                     KIVTNSYSDFNPHMGKIVRKFFDESWIHAPVLKGKSPGAFSASTVPSVHPYILVNYQG
                     KMRDVSTLAHELGHGIHQYLAGKKQGHFNASTPLTLAETASVFGEMLTFKSILTNEKD
                     PKEKKALLANKVEDMLNTVVRQIAFFEFEKRIHEKRKKSELSVKEICSIWLETQKESL
                     GPSINFEDEYKYYWTYIPHFIHSPFYVYAYAFGDCLVNSLYDVYQDGLEKFDKKYIQL
                     LEAGGSMRYRELLKPFNLDPSQPGFWQKGISVIESFIDQLNEIR"
BASE COUNT          354 a          210 c          169 g          409 t
ORIGIN      
        1 aatatttcca agagcgtttc taacttcagc tgcgtgtttt cttttatcta atttaacacc
       61 aagatatttt tgaccagcaa gtttagtagc taacgcgcca actatacctc caactttagc
      121 atagcgtttt atttgtttgg caaaagattt tgattctcta tctttcattt tataaaatca
      181 tctaatttca tttaattggt caataaaaga ctcaattact gaaatacctt tttgccaaaa
      241 acctggttgg gacggatcta agttgaatgg ttttaacaat tccctgtaac gcattgatcc
      301 tccagcttct aacaactgaa tatatttttt atcgaatttt tctaaaccat cctgatagac
      361 gtcatataag gagttaacaa gacaatcgcc aaaggcataa gcgtaaacat aaaaaggaga
      421 gtgaataaag tgcggaatat atgtccaata atatttatat tcatcttcaa aattaattga
      481 tggacctaaa ctttcttttt gtgtctctag ccatattgaa caaatttcct ttacgcttaa
      541 ttctgatttt ttccttttct catgtattct tttttcaaat tcaaaaaaag caatttgtct
      601 gacaacagta tttaacatgt cctccacttt attcgctaat aatgcctttt tttccttagg
      661 atctttttca ttggttaata tcgatttaaa tgtaagcatt tcaccaaaaa cacttgcagt
      721 ttcagctaaa gtaaggggcg ttgacgcatt aaaatgacct tgctttttcc cagctaaata
      781 ttgatgtatt ccatgcccta attcatgagc taaagttgaa acatctctca tcttcccttg
      841 gtaattgacc aaaatatagg gatgaacact aggaactgtt gaagctgaaa aagcacccgg
      901 tgattttccc ttgagcacag gtgcatgtat ccaggattca tcaaaaaatt ttctcactat
      961 tttccccatg tgaggattaa agtctgaata agaattagtg acaatttttt ttgctttatt
     1021 ccaagtaaat tgttttttgc tttgaaaagg taaaggagca tttctgtccc aatattttag
     1081 cgactttacc ccaaaccatt ttgctttcat ggcataataa cgatgtgaca aattggagta
     1141 tg
//
LOCUS       DTVV01000257            1242 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1734547, whole genome shotgun sequence.
ACCESSION   DTVV01000257 DTVV01000000
VERSION     DTVV01000257.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1734547"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1242)
                     /locus_tag="EYO86_03050"
     CDS             complement(<1..>1242)
                     /locus_tag="EYO86_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIC42044.1"
                     /translation="NTSTKIPENTRAITGISCAYLVQNMERNNEGFTQMGSEGASWVG
                     ESVFSNTDHIFQNMGDGTYIHSGILSIRHAVAAKTKMTFKILYNDAVALTGGQALDGL
                     PTVAQMSRQLESEGVKKIAIITDEPNKYKSRDKFSNNSRVYDRKEIIDVQIKLSQIND
                     TTAIIYDQTCAAEKRRRIKKSILPEPNKKIFINEKVCEGCGDCGIQSNCISIVPVETE
                     YGRKRQIDQSNCNKDYSCVDGFCPSFVSVVGDVKLKTNVNNSLIERINSTISEPILPE
                     IQQSYGIMIAGIGGTGVVTIGAILATAAQIDGKGSGVLDMTGLAQKGGAVKSFLRIFN
                     DPKEISTIRLSYGDTNLLMGFDLLVANDEEVLKTLDKQKTKSIINSDEIMTGEFTRDK
                     NFFLPFDKMKENLINILGKNN"
BASE COUNT          362 a          232 c          159 g          489 t
ORIGIN      
        1 atattatttt taccgaggat atttattaaa ttttctttca ttttatcaaa tggaaggaaa
       61 aaatttttat ctcttgtaaa ttctccagtc attatttcat cggaattaat aattgatttt
      121 gttttttgtt tatccaaggt ttttaatact tcttcatcat tagctacaag taagtcaaag
      181 cccattaata aattggtatc accatatgaa agtcttattg tacttatttc ctttggatca
      241 ttaaaaattc ttaaaaaact tttaactgcc ccgccttttt gggcaagacc cgtcatgtca
      301 agaacacctg atcctttacc atctatttgc gccgctgtag ctagtatagc tccaattgtt
      361 actacacctg tacctccaat tcctgctatc ataatcccat atgattgttg aatttcaggt
      421 aaaattggtt cagaaatagt tgaatttatt ctttctatta gtgaattatt aacatttgtt
      481 tttaatttaa catctcctac aactgacaca aagcttgggc aaaatccatc tacacacgaa
      541 taatctttat tgcaattaga ctgatctatt tgtcttttgc gtccatattc tgtttctact
      601 ggaacaattg aaatgcaatt tgattgtatt ccacaatcac cacatccttc acatactttt
      661 tcatttataa atattttttt atttggctca ggtaatatag atttttttat cctacgtctt
      721 ttttcagcag cacatgtttg atcataaatt atagccgtag tatcattaat ttgactgagc
      781 tttatttgaa catcaattat ttcttttcta tcatatactc ttgaattatt agaaaattta
      841 tctcttgatt tatatttatt tggttcatct gtaataattg ctattttttt aactccttct
      901 gattcaagtt gtctagacat ttgcgcaaca gttggtaatc catcaagagc ctgcccccct
      961 gttaaagcaa cagcatcgtt atatagaatt ttaaatgtca tttttgtttt ggctgccaca
     1021 gcatgtctga tagataaaat gcctgaatga atatacgttc catctcccat attttgaaaa
     1081 atatggtctg tattactaaa aacagattca ccaacccagc tagccccttc agaacccatc
     1141 tgcgtaaaac cttcattatt tctttccata ttctgaacta gataagcgca gctaatacca
     1201 gtaatagctc ttgtattttc tgggatcttt gttgaggtat tg
//
LOCUS       DTVV01000258            2252 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1746536, whole genome shotgun sequence.
ACCESSION   DTVV01000258 DTVV01000000
VERSION     DTVV01000258.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1746536"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /gene="rpsN"
                     /locus_tag="EYO86_03055"
     CDS             <1..175
                     /gene="rpsN"
                     /locus_tag="EYO86_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIC42045.1"
                     /translation="QTKLNDLPRDSANVRVRNRCELTGRSRGTYRKFGLSRIKLRELS
                     MAGLLPGVVKSSW"
     gene            188..586
                     /gene="rpsH"
                     /locus_tag="EYO86_03060"
     CDS             188..586
                     /gene="rpsH"
                     /locus_tag="EYO86_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIC42046.1"
                     /translation="MSFNDSLSDMLARIKNAHKANKTFASCYKSKLNSNVLTVLKDEG
                     YIRDFQDVELRKGISNIKIDLKYYNGIPVIRKIKRVSKPGIRIYSKINDLPKPYGGLG
                     ISILSTPKGVMSDKKARDNNVGGEILCEVF"
     gene            590..1123
                     /locus_tag="EYO86_03065"
     CDS             590..1123
                     /locus_tag="EYO86_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIC42047.1"
                     /translation="MSRIAKNSIKIPNDTTCKFENSVFFAKGKLGEMSMIVNSLFNVN
                     LKDGEIHVLPKNEKDKSNPIWGTTRALVSNVVIGVTEGFSKTLELNGTGYRASISGSK
                     LKLQLGYSHDVNFEVPKEVKVECPKQNIIKLSGSNKEILGAVAANIRSFRKPEPFKGK
                     GIKYVNEFIFRKEGKKK"
     gene            1127..1465
                     /locus_tag="EYO86_03070"
     CDS             1127..1465
                     /locus_tag="EYO86_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013695848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIC42048.1"
                     /translation="MKNSTRQNRTRFKLKKVTDRKRLSIFKSNNHIYAQIINDEKGVT
                     LASASSLEKSIRTENKTKKELAERIGKEIAKRSIENGIKEVAFDKGKYKYHGIIKILA
                     EAARAEGLNF"
     gene            1474..1980
                     /locus_tag="EYO86_03075"
     CDS             1474..1980
                     /locus_tag="EYO86_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIC42049.1"
                     /translation="MFENENKEFSEHLVTINRVAKVVKGGRRFGFAAIMIVGDNKGRV
                     GYGTGKAKEVPEAVRKATEDAKTKMVRVHLKDNRTLHHDIVGRFGAGKVILRSAAPGT
                     GIIAGGPMRALFESLGIKDVVAKSTGSSNPHNMIKATLDAFKRSESPKSVAAKRSKKI
                     SDIIKVKE"
     gene            1988..>2252
                     /gene="rplO"
                     /locus_tag="EYO86_03080"
     CDS             1988..>2252
                     /gene="rplO"
                     /locus_tag="EYO86_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIC42050.1"
                     /translation="MKINELKSTLTSNTKRKRVGRGSGSGLGKTAGRGYNGQKSRSGI
                     AIKGFEGGQMPLYRRLPKRGFKNIFSKKIQSINFNQINHFIKKY"
BASE COUNT          902 a          270 c          438 g          642 t
ORIGIN      
        1 acaaacaaaa ttaaacgatc ttcctagaga tagtgcaaat gtaagagttc gcaatcgatg
       61 tgagctaacg ggtcgcagta gaggaacata tagaaaattt ggcctttcaa gaattaaact
      121 aagagaatta tcaatggctg gcttactgcc aggtgttgtt aagtcgagtt ggtaaggaga
      181 gaagattatg tcatttaatg actcattatc ggatatgttg gcaagaatta aaaatgcaca
      241 caaagcaaat aaaacatttg cttcttgcta taaatctaaa cttaattcaa atgttttgac
      301 tgtactaaaa gacgaaggat atataagaga ttttcaagat gtagaattgc gaaagggtat
      361 aagcaatatt aaaattgatt tgaagtatta taatggaata cctgttataa gaaaaattaa
      421 aagagtatca aaaccaggta ttcgtattta ttcaaaaatt aatgatttgc caaagccata
      481 tggaggattg ggtatatcaa ttttatcaac tccaaaagga gtaatgtctg ataaaaaagc
      541 tagagataat aatgttggtg gcgaaatatt gtgtgaggta ttttaattca tgtcaagaat
      601 agcaaaaaat agtattaaaa ttcctaatga tacaacatgc aaatttgaaa atagtgtgtt
      661 ttttgcaaaa ggaaaactag gtgaaatgtc tatgatcgtt aactctcttt ttaatgtaaa
      721 tttaaaagat ggtgagattc atgtgttacc caagaatgaa aaagacaagt ctaacccaat
      781 ttggggaaca acgagggcac ttgtttctaa cgttgtcata ggagtgaccg agggtttttc
      841 aaaaacatta gaattaaatg ggactggata tagagcttct atctctggat ctaaattaaa
      901 attacaatta ggttatagtc atgacgtaaa ttttgaagtt cctaaagaag taaaagtcga
      961 atgccccaaa caaaatatta ttaaattaag tggatccaat aaagaaattt taggagctgt
     1021 tgcagctaat attcgatcgt ttaggaagcc tgaacctttt aagggtaagg gcatcaaata
     1081 tgtaaatgaa tttattttta gaaaagaagg taagaagaaa taaattatga aaaattctac
     1141 tagacaaaat agaactagat ttaagttaaa aaaagtaaca gataggaaaa gattgtcgat
     1201 atttaaatct aacaatcata tctatgctca aataataaat gatgaaaaag gagttacttt
     1261 ggctagtgct tcatccttag aaaaatctat acgcacagaa aataaaacta aaaaagaatt
     1321 agctgaaaga attggaaaag aaattgcaaa acgttcgatt gaaaatggaa ttaaagaagt
     1381 tgctttcgat aaaggtaaat ataaatatca tggcatcatt aaaatattag ctgaagcagc
     1441 aagagcagaa gggttaaact tttagaggat tatatgtttg agaatgaaaa caaagaattt
     1501 agtgaacatt tagtgacaat aaatcgtgta gcaaaagtcg ttaaaggtgg ccgtcgtttt
     1561 ggttttgcag ctataatgat tgttggagat aataaaggac gtgttggata tggaaccgga
     1621 aaagcaaaag aagttcctga agctgttcgc aaagcaactg aggatgctaa aactaaaatg
     1681 gtccgagttc atctaaaaga taatcgaaca ttgcatcatg atattgtagg tcgttttgga
     1741 gcaggtaaag ttattttgcg ttcagccgca cctgggacag gtataattgc tggaggacca
     1801 atgagagcgt tatttgaatc ccttggtatt aaagatgttg ttgcaaaatc tactggaagt
     1861 tccaatccac ataatatgat taaagctaca ttggatgcat ttaaaagatc agaatctccg
     1921 aaaagtgttg cggcaaaaag atcaaaaaaa atttcagata taataaaagt taaggaataa
     1981 aattataatg aaaattaatg aactaaaatc aactcttact tcaaatacaa aaagaaaaag
     2041 agtaggcagg ggatcaggat ctggtttagg taaaacagcc ggacgaggat ataacgggca
     2101 aaaatcaaga tcaggcattg caatcaaagg ttttgaaggt gggcaaatgc ctttatacag
     2161 aagacttcca aaaagaggat ttaaaaatat attttcaaaa aaaatacaat ctataaattt
     2221 taatcaaatt aatcatttta ttaaaaagta ta
//
LOCUS       DTVV01000259            1122 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1749915, whole genome shotgun sequence.
ACCESSION   DTVV01000259 DTVV01000000
VERSION     DTVV01000259.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1749915"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(6..91)
                     /locus_tag="EYO86_03085"
     tRNA            complement(6..91)
                     /locus_tag="EYO86_03085"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(55..57),aa:Tyr,seq:gta)
     gene            267..1043
                     /gene="rlmB"
                     /locus_tag="EYO86_03090"
     CDS             267..1043
                     /gene="rlmB"
                     /locus_tag="EYO86_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIC42051.1"
                     /translation="MKSNNKRKSNNKRLAEIFGTHAVHAALQNPSRKHEKLFISHHQR
                     KILGKGIEKLVSDINELHNKEMFKMFGNESTHQGIVLRTSKLQQPSLQEFINDTYKNE
                     TDVVVMLDQVTDPNNIGSIMRSCSLFNSKSIIVSNDNAPDVTATMAKAASGALESVNY
                     ISVVNLSRAMIQFKKNNYWIYGFDSNKNNNQNINLDLPKKCVLVFGSEGKGLRNLIKK
                     ECDTLVSIPMQTHEKYSIDSLNVSNACSIALYEHFKNNIK"
     gene            complement(1045..1120)
                     /locus_tag="EYO86_03095"
     tRNA            complement(1045..1120)
                     /locus_tag="EYO86_03095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1085..1087),aa:Thr,seq:tgt)
BASE COUNT          422 a          187 c          185 g          328 t
ORIGIN      
        1 caaattggtg gagggggtag gatttgaacc tacgtacgct cacgcgggcg gatttacagt
       61 ccgctgccat taaccactcg accacccctc caattttgaa gaaaatggca catactaagt
      121 aaatagtatg attgcaatct aaaacagcga tatctccagc gcaaacatta taaattctta
      181 acagcgggct ccttttagac aaattttgct aatttgtata gatctagatt atattaaata
      241 agtatatctg aaatatatct taataaatga aatcaaataa taaaaggaaa tcaaataaca
      301 agagattagc tgaaatattt ggcactcatg ctgtccacgc tgctctacaa aatccaagta
      361 gaaaacacga aaaattattt atatctcatc atcaaagaaa gattttaggt aaaggtattg
      421 aaaaattagt ttctgatatc aatgagttac ataataaaga aatgtttaaa atgtttggaa
      481 atgaatcaac gcaccaagga attgttctga gaacatctaa attgcaacag ccaagtttac
      541 aagaattcat taatgatact tataaaaatg aaacagatgt cgtcgttatg cttgatcaag
      601 taacagaccc taataatata gggtcaatta tgaggtcctg ctcattattt aattctaaat
      661 caattattgt ctcaaatgac aatgcaccag atgtaactgc tactatggcg aaggctgctt
      721 ccggagcact tgaaagtgta aattatattt cagttgtcaa cttatcgcga gctatgattc
      781 aatttaaaaa aaataattat tggatttatg gatttgacag caataaaaac aacaatcaaa
      841 atataaattt agatttacca aaaaagtgcg ttctagtttt tggatcggaa ggaaaaggtt
      901 tgcgtaatct cataaaaaaa gaatgtgata ccttagtctc aataccaatg cagactcatg
      961 aaaaatatag tattgacagt ttaaatgtat ctaatgcttg ttctatcgct ctttacgaac
     1021 actttaaaaa taacattaaa taaatggtgc cgcgtgtcgg aatcgaactg accacctgat
     1081 gattacaaat caactgctct accaaatgag ctaacgcggc ac
//
LOCUS       DTVV01000260            3290 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1755770, whole genome shotgun sequence.
ACCESSION   DTVV01000260 DTVV01000000
VERSION     DTVV01000260.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1755770"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="EYO86_03100"
     CDS             complement(<1..239)
                     /locus_tag="EYO86_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIC42052.1"
                     /translation="MSTSKVLEEANSIAEKIAIFNQEDENPYHQIKQKISEKNIKHIV
                     TVARGTSDCAALYASYLFAKTLGLTTYSLPPSIITL"
     gene            complement(236..1381)
                     /gene="nagA"
                     /locus_tag="EYO86_03105"
     CDS             complement(236..1381)
                     /gene="nagA"
                     /locus_tag="EYO86_03105"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:PF01979.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIC42053.1"
                     /translation="MQINFFIKGPFKAALYVFVNKISGKIINYDGSYTGTIEFSDKIE
                     SIDIDKDKQSDFENIIIPGFIDLHCHGGNGFDTMAGFSSIIEMSQYHLKQGTTTLLPT
                     TLTATLDDTVKALEGLGDFIDQNNNLTNILGVHLEGPFINPNKLGAQPEYAQLPNIKF
                     IEKINDKVKIKVITLAPELEGADSFIDYLINNNINVQIGHSLADYNCCMKIMNKNKVG
                     FTHLYNAMSGGDHRKPGVVTAALRHAEFAEIICDLYHVDQENIHLAHKCIPKLYAISD
                     AISASGMPDGQYDFANTKITKKNGKALINFDVLAGSVINMHDTFKNLVKINFSLEKAV
                     AMTSFNAAQYISENNKGKIDKGFCSNLVVLDKQLNIKKVYLHGSLIS"
     gene            complement(1480..>3290)
                     /locus_tag="EYO86_03110"
     CDS             complement(1480..>3290)
                     /locus_tag="EYO86_03110"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00728.20,HMM:PF02838.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42054.1"
                     /translation="KLCLSLVYSIKKVEGAQIIKQIGRYYELLLDQSNLGPKKIITLV
                     LQLQTPRIGTYNLSCGPEGLFILDINEKRIDFEILQLSFDTIINQSQYAAVSEDINNN
                     VIPELKKTKFSNQYLSIPQNNFVTEDEKLLEIFKFLKPTCDLLSINFFSAGGQHIKFQ
                     SMKLENDEYRINISQNGITIYANDYGGRFYAIITLIHLISYYDSQLPLGKIEDRPYFA
                     WRGMHLDCSRQFHTVEQIKRLLIYMGMFKLNRFHWHLTDNEAWRLDLKCYPNLARQSS
                     FRGYKQLIPPLYGSGFEKSGGYYSNEEVKDIIAFAKKLNIEVMPEIDLPAHSWALTQV
                     MPELYDHSSNVLSEDVGSYKNNTINPSLESTWNFLNNIIAEISDLFSFHIIHVGVDER
                     PKSSWEGSPKIQQLMKEKNFQSFDEVQDYYMSRVINIIKKKNKRTAAWNEAALSPHND
                     IGSSGGTGKIDKSCLIFAWEHPSVSNESAKRGFQTVMCPGQKTYFDMAYNNATSERGI
                     SWAATIEASEIHSWKPLKDVDKDYHNLILGIQGQLWSETITKPEYIDTLINPRLACLS
                     ELAWCSDNRRGWAEFKPTLLHNMKILSKLGWKYHNF"
BASE COUNT         1017 a          585 c          503 g         1185 t
ORIGIN      
        1 agagtaatga tcgatggcgg caaactataa gttgtcagcc ctaatgtttt tgcaaacaaa
       61 taagaggcgt atagtgctgc acagtcagaa gtgccccttg ccacagtaac tatatgctta
      121 atattttttt cggatatttt ttgtttaatc tggtgataag gattttcatc ctcctgatta
      181 aaaattgcaa ttttctcagc aatactattt gcctcttcaa gaaccttgct agtactcatg
      241 atattaaact tccatgtaaa tatacttttt tgatgtttaa ttgcttatct aaaactacca
      301 aattagaaca aaatcctttg tctatctttc ctttattatt ttcactaata tattgtgccg
      361 cattaaagct tgtcattgct acggcttttt ctagtgaaaa attaattttc actaaatttt
      421 taaacgtatc atgcatattt ataacactac cggctaaaac atcaaaatta atcagagcct
      481 ttccattttt ttttgttatt ttagtatttg caaaatcata ttgtccgtct ggcatcccac
      541 ttgcagatat ggcatcagat atggcataaa gtttggggat acatttatgg gcaagatgaa
      601 tattttcttg atctacatga tataaatcac aaattatttc agcaaattct gcatgtctaa
      661 gagcagctgt taccacaccc ggcttacgat gatcaccgcc tgacatagca ttatataagt
      721 gagtaaaacc taccttgttt ttattcatta ttttcataca acaattataa tcagctaaag
      781 aatggccaat ttggacatta atgttattgt ttattaaata gtctataaag gaatccgccc
      841 cttccaattc tggagctaga gtaattactt taattttcac tttatcatta attttttcaa
      901 taaatttaat attaggaagt tgagcgtact cgggttgtgc tcctaattta tttggattaa
      961 taaaaggacc ctcaagatgt acacccaata tatttgttaa attattattt tggtcaataa
     1021 aatcaccaag gccttcaagt gctttaacag tgtcatcaag agttgcagtt agggttgttg
     1081 gcaataatgt cgttgtacct tgtttaagat gatactgaga catttctata attgaagaaa
     1141 atccagccat agtatcaaat ccatttccac cgtgacaatg aagatctatg aaacctggta
     1201 ttattatatt ttcaaaatct gattgcttgt ctttatctat atctatggat tctatcttat
     1261 cactaaattc aatagtgcca gtatatgaac catcgtaatt tataattttt cctgaaattt
     1321 tattcacaaa aacataaagg gccgctttaa acggtccttt gataaagaaa tttatttgta
     1381 ttcttcaagc tcctctgctg cttccgcaac caaatccaac cgcaccaatt ttcatgataa
     1441 gtaattatgt aaatttttta atgttcacaa gtacaatgat caaaaattat ggtatttcca
     1501 ccctaattta cttaatattt tcatattatg caaaagagtt ggtttaaatt ccgcccatcc
     1561 tctcctgtta tctgaacacc aagctaattc tgataaacat gcaaggcgag gattgatcag
     1621 ggtgtctata tactctggtt tggttatagt ttccgaccac agttgacctt ggatacctag
     1681 aattaaatta tgataatctt tatcgacatc ttttagaggc ttccatgagt gaatttccga
     1741 tgcttcaatt gttgctgccc agctaattcc cctttctgat gttgcattgt tatacgccat
     1801 atcaaaataa gttttttgtc caggacacat aactgtttga aaacctcttt ttgcagactc
     1861 atttgaaact gaagggtgct cccatgcaaa tattaagcag cttttatcta tttttcctgt
     1921 tcctccactt gaccctatgt cattgtgcgg tgataacgca gcttcattcc atgcagcggt
     1981 tcttttgttt tttttcttaa taatattgat gaccctactc atgtaataat cctgaacctc
     2041 atcaaatgat tgaaaatttt tctctttcat caattgctgt atcttgggag acccttccca
     2101 tgatgattta ggacgctcat ccactccgac atgaatgata tgaaatgaaa ataaatcaga
     2161 tatttcagca attatattat tcaaaaaatt ccaagttgat tctaacgagg gattaatagt
     2221 attgttttta taacttccaa catcctcaga aagcacatta gaactatgat cataaagctc
     2281 gggcattacc tgagttaaag cccatgaatg ggccggtaaa tctatttcag gcataacttc
     2341 gatattcaat tttttagcaa atgctattat atcttttact tcttcattgg aataatatcc
     2401 gcctgacttt tcaaaaccac taccgtacaa agggggaatg agttgtttat atccacgaaa
     2461 agatgattgt ctggctaaat tgggataaca ttttaaatca agtctccatg cttcattatc
     2521 agttaaatgc caatgaaaac gattaagctt gaacattccc atgtaaatca aaagacgttt
     2581 tatttgttca accgtatgga attgtcgaga acaatctaga tgcattcctc gccatgcgaa
     2641 atatggtctg tcttctattt tccccaatgg taattgagaa tcataatatg agatgagatg
     2701 aattaacgtt atgatggcgt aaaaacgacc tccataatca ttggcataaa ttgttatgcc
     2761 gttttgggaa atattgattc tgtactcatc attttccaat ttcatcgatt gaaactttat
     2821 gtgttgtcct cctgcagaga agaaattaat ggacaacaag tcacaagttg gcttaagaaa
     2881 tttaaaaatt tctagtaatt tttcatcttc tgttacaaaa ttattttgcg ggattgatag
     2941 atactgattt gaaaattttg ttttctttag ctcaggaata acattgttgt ttatatcttc
     3001 tgaaactgct gcatactgag attgatttat tatggtatca aaacttagct gaagaatttc
     3061 aaaatcaatt cgcttttcat tgatatccaa aataaaaagg ccttcgggtc cgcaggataa
     3121 attgtatgtt ccaattcttg gtgtctgaag ttgtaatacc agagttataa ttttcttggg
     3181 tcctaggttg gattgatcta ataataattc gtaatatctt cctatttgct taataatttg
     3241 agcgccttca acttttttaa tggaataaac caagcttaaa cataatttta
//
LOCUS       DTVV01000261            1170 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1756525, whole genome shotgun sequence.
ACCESSION   DTVV01000261 DTVV01000000
VERSION     DTVV01000261.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1756525"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="EYO86_03115"
     CDS             complement(<1..399)
                     /locus_tag="EYO86_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIC42055.1"
                     /translation="MPIRDIFLAILVPIIFGFGFVIAKPAMDQLPPILLNGIRLSLSG
                     IIMFWFFPFPKKLFKSLLIISFLGSTLQYSLTFSGLNIVDATSATLLIQCEIPFGILI
                     AFLILKEKPSLKNLLGLLIAFIGIIFLTGSP"
     gene            complement(415..>1170)
                     /locus_tag="EYO86_03120"
     CDS             complement(415..>1170)
                     /locus_tag="EYO86_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC42056.1"
                     /translation="LKKMISDEGAETIAAFIGEPILGTGGIIPPPKGYWEAVQKVLED
                     NDIMFIVDEVVTGFGRLGTMFGSEYYNLRPDIITIAKGLTSAYAPLSGSIFSNKVWKV
                     LEQGTDELGPIGHGWTYSAHPIGAAAGVANLELVDKMNLVSNAKEVGKYFLDELKNAF
                     DPHPLVGEVRGDGMLASLEFLENKESLKFFDPAKKIGMKVSANLLKEGVIGRAMPHGD
                     ILGFAPPLCLSKSEVDIIVSSLKKSVDKVHSEI"
BASE COUNT          403 a          231 c          179 g          357 t
ORIGIN      
        1 cggactacct gttaaaaaaa ttattccaat aaaagcaatc aacaagccta atagattttt
       61 taaggagggt ttttctttta aaatcaaaaa ggctatgaga attccaaatg gaatctcaca
      121 ttgtattagt aatgttgctg aagttgcatc aacaatattt aagcctgaaa aagttaatga
      181 atattgaaga gtagaaccta aaaaagaaat tattaataat gatttaaata atttttttgg
      241 aaatggaaaa aaccaaaaca taataatccc gcttaaagac aaacgtattc cgttaagtaa
      301 aataggaggt aactgatcca tagcaggttt tgcaattaca aaaccgaacc caaaaattat
      361 tggcacaagt attgctagaa aaatatctct aattggcatg atatttaaaa tttattaaat
      421 ttcgctatga actttatcta ctgatttttt taaacttgat acaataatat ctacttcaga
      481 tttgcttaaa cataaaggag gggcaaaacc taatatatct ccgtgtggca ttgctcgtcc
      541 gatcacacct tctttaagta aattagcgga aactttcatt ccaatttttt ttgcggggtc
      601 aaaaaatttt aaactttcct tattttctaa aaactcgaga gacgccaaca ttccatcgcc
      661 cctaacttcc ccaaccagag ggtgagggtc aaatgcgttt tttaactcgt caagaaaata
      721 tttgcctact tcttttgcat tagaaaccaa attcatttta tctactagct ctaaattcgc
      781 tactcctgcg gcagctccaa tgggatgagc agaataagtc cagccatgtc caattgggcc
      841 taattcatct gtgccctgct ccaatacttt ccaaacttta tttgaaaaaa tagagcctga
      901 taaaggcgca taagcggagg ttaatccctt tgctatagta ataatgtcag gacgaagatt
      961 gtagtactca gacccaaaca tagttcccaa acggccaaaa cctgtaacaa cctcatcaac
     1021 tataaacata atgtcattat cttccaaaac tttttgaaca gcctcccagt atcccttcgg
     1081 tggcggtata atgcctcctg taccaagaat tggctctcca atgaaagcag caatggtttc
     1141 tgccccttca tcactgatca tttttttaag
//
LOCUS       DTVV01000262            1022 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1758530, whole genome shotgun sequence.
ACCESSION   DTVV01000262 DTVV01000000
VERSION     DTVV01000262.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1758530"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(49..450)
                     /gene="cdd"
                     /locus_tag="EYO86_03125"
     CDS             complement(49..450)
                     /gene="cdd"
                     /locus_tag="EYO86_03125"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIC42057.1"
                     /translation="MISEYFVQMFEAATKVRERAYVPYSKFKVGAAILTEDNHIVVGC
                     NAENAAYPQSQCAEASAIGNMVSQGYSIIKEVLVIGSGDLLCSPCGACRQRIREFASL
                     KTPIHMCNIKEHLKTSTLEELLPDSFGPENL"
     gene            complement(466..>1022)
                     /locus_tag="EYO86_03130"
     CDS             complement(466..>1022)
                     /locus_tag="EYO86_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIC42058.1"
                     /translation="LKERPSIKNLIGLLIAFTGLIVLLGAPNLQDKYLGVILVLSGAF
                     TWSLGQVFAKPISEKLNGIVITAWLGVLAGPQLILASQLIEGNVVSNIRSADYQAWLI
                     VLYLGLLMNVLGYSIWYYLLGIYPVNKILPIMLLLPVTGVVTAIMFLGERPDLKVFMG
                     GIVILFGVGMILIGKIKNQRVKHI"
BASE COUNT          350 a          197 c          151 g          324 t
ORIGIN      
        1 actcaatatc atttgttttt tttttaaaaa tttttgagga tgaaagcatt ataaattctc
       61 tggaccaaaa gaatcaggaa gtaattcttc taaggttgag gttttcaggt gttctttgat
      121 attacacata tgaataggtg tttttaaaga agcaaattca cgaatgcgtt gacgacatgc
      181 accacacggg gaacataata aatcacctga acctatcact aatacttctt taattattga
      241 gtatccttgg ctcaccatat ttcctatagc gcttgcctct gcacattgag attgaggata
      301 ggcagcattt tctgcattac aacctacaac aatgtgattg tcttctgtta agatagcagc
      361 accaacttta aatttagaat aaggtacata cgctctttct ctaacctttg ttgctgcttc
      421 aaacatttgt acaaagtact cagaaatcat tattcacttt accatttaaa tgtgctttac
      481 gcgttgattt tttattttac caattaaaat cattccaaca ccaaaaagaa ttacgatgcc
      541 acccataaat acttttaaat ctggtctttc tccaagaaac ataatagcag tgactactcc
      601 tgtgacggga agtaataaca taattgggag tattttattt actggataaa tgcccagtaa
      661 ataataccaa atgctgtagc ctagcacatt cattagtaat cctaaatata aaactatgag
      721 ccaggcctga taatcagcag atctaatatt actcactaca ttgccttcaa taagttgaga
      781 agcaagaatt aactgtggtc cagctaatac tcctagccaa gcagtaataa caattccatt
      841 aagcttttca ctaatgggct ttgcaaaaac ttgtcctaaa gaccaagtaa atgcaccact
      901 caaaactaaa ataacaccaa gatatttgtc ctgaaggttt ggagcgccta ataaaacaat
      961 taaaccagta aaagcaatta aaagcccgat tagattttta atactgggtc tttcttttaa
     1021 cc
//
LOCUS       DTVV01000263            1057 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1759931, whole genome shotgun sequence.
ACCESSION   DTVV01000263 DTVV01000000
VERSION     DTVV01000263.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1759931"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(100..705)
                     /locus_tag="EYO86_03135"
     CDS             complement(100..705)
                     /locus_tag="EYO86_03135"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIC42059.1"
                     /translation="MQSIGDTKKIIVDRFLGVEIINNDKYLEIKTEPVKIFKVIKELK
                     EDTKLLFDQLIDVTAIDYPSREKRFDIIYLLISLTLNQRILVKTSIDESTVLESITIV
                     HKAANWYERECYDLFGIQFSNHPDLRRIMTDYNFEGHPLRKDFPLTGHTEVHYDDLEK
                     KVVYEPVKLAQEYRNFDYMSPWEGFDNKLIGDEKSSEENNS"
     gene            complement(707..793)
                     /locus_tag="EYO86_03140"
                     /pseudo
     CDS             complement(707..793)
                     /locus_tag="EYO86_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248418.1"
                     /note="The point of entry for the majority of electrons
                     that traverse the respiratory chain eventually resulting
                     in the reduction of oxygen; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
     gene            complement(888..>1057)
                     /locus_tag="EYO86_03145"
     CDS             complement(888..>1057)
                     /locus_tag="EYO86_03145"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_426642.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIC42060.1"
                     /translation="AILFIIFDLEVAFLFPWAISLGSIGIFGFWSMMIFLLILTVGFI
                     YEWKKGALEWE"
BASE COUNT          341 a          191 c          133 g          392 t
ORIGIN      
        1 ccacttcccc atctaattcc acaaccaatc ttaaaacacc atgtgctgcc ggatgttgag
       61 ggccaaaatt aagagtagtt gagttaattt ctacttcttt catgagttat tttcttctga
      121 ggatttttca tctcctatta atttattatc aaacccttcc caagggctca tataatcaaa
      181 atttctatat tcttgtgcta gcttgacagg ttcgtacaca actttttttt caaggtcatc
      241 ataatgaact tctgtatgtc ctgttaaagg aaaatctttt cttagcggat gtccttcaaa
      301 attataatct gtcattattc ttcgtaaatc tgggtgatta gaaaattgaa taccaaataa
      361 atcataacat tctctttcat accaatttgc tgctttatga acaattgtaa ttgattccaa
      421 tactgtactt tcatcaatag atgttttaac taaaatgcgt tgatttaaag ttaaacttat
      481 taataagtaa ataatatcaa atcgcttctc tcttgaagga tagtcaattg ctgtcacatc
      541 aattaattgg tcaaatagta gttttgtatc ttcctttaat tctttaatga ctttgaatat
      601 ttttaccggt tcagttttga tctctaaata cttatcatta ttaattattt caactccaag
      661 aaatctatca actataattt tttttgtatc accaatgctt tgcatcttat ctgttaaata
      721 tattttttct ttttattttc ttttgaagtt gcaaaatacc gtaaaccaaa gcttttgctg
      781 tgggtgggaa cccgttcgag cccaagttat taatttatca aagtttgcta aaagaaaacc
      841 tttagaattt aattcatctc caaaatcatt ttcatcaatc gtggaatcta ctcccattct
      901 agtgcgcctt ttttccattc atatataaag ccaacagtaa gtattaataa gaaaatcatc
      961 atagaccaaa atccaaaaat cccaatgcta ccgagtgaaa ttgcccaagg aaataggaag
     1021 gcgacttcta gatcaaaaat aataaataag attgcta
//
LOCUS       DTVV01000264            2205 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1764805, whole genome shotgun sequence.
ACCESSION   DTVV01000264 DTVV01000000
VERSION     DTVV01000264.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1764805"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="EYO86_03150"
     CDS             complement(<1..572)
                     /locus_tag="EYO86_03150"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867681.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HIC42061.1"
                     /translation="MRRVVVTGLGIISCIGNNQETVIKNLKNLNSGITKAPEYEEFSF
                     RSLVHGKPDINLGEQIDRRILRFMGDGAAYNYIAMKDAIKDSGLEDGEVSNEMTGIIV
                     GSGGPSTQNLLKSSELGKEGGSRKVGPFMVPRTMSSTNSATLATPFKIKGVNYSITSA
                     CSTSSHCIGNAYELIQIGKQKIIFAGGGEE"
     gene            complement(579..1085)
                     /gene="fabA"
                     /locus_tag="EYO86_03155"
     CDS             complement(579..1085)
                     /gene="fabA"
                     /locus_tag="EYO86_03155"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HIC42062.1"
                     /translation="MKEDSFNYEKLIECGKGILFGKGNAQLPLPPMLMFDRITHINEI
                     GGEFAKGEVIAELDIESNLWFFDCHFKNDPVMPGCLGLDAMWQLVGFYLGWLGQLGKG
                     RALGVGEVKFTGQVLQTVKKVTYHISLKRLILRKLILGVADGILKADGKTIYEAKDIK
                     VGLFNTSK"
     gene            complement(1144..1884)
                     /locus_tag="EYO86_03160"
     CDS             complement(1144..1884)
                     /locus_tag="EYO86_03160"
                     /inference="COORDINATES: protein motif:HMM:PF00899.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42063.1"
                     /translation="MNITEEELEIFSRQLILKDFKEEKFNELQKKEISIIGLGGIGCP
                     LAQYLISAGIKKLNIFDGDTIKKTNLNRQTLYSIHDIGKKKSTIAKKKLLGTNPNAVI
                     NSYNHKIAKDNLHLLKNSSIIIDASDNWETMRLINKYTTKKNLPLLSISVVGFDIQMI
                     LFENTTHNHLCLECIFPNHNEPELARCDTVGIMGTTAGLAGIISAQKAINFLLKFNEK
                     NNILTLVDVKLLSISHIKTKKNIDCKLQ"
     gene            complement(1890..>2205)
                     /locus_tag="EYO86_03165"
     CDS             complement(1890..>2205)
                     /locus_tag="EYO86_03165"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:PF04127.13"
                     /note="catalyzes the conjugation of cysteine to
                     4'-phosphopantothenate to form
                     4-phosphopantothenoylcysteine, which is then
                     decarboxylated to form 4'-phosphopantotheine; in the
                     alphaproteobacteria, this protein contains an N-terminal
                     SbtC-like domain; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate synthase"
                     /protein_id="HIC42064.1"
                     /translation="LKNIILKKNRDILQNISSLKKNRPNIVIGFAAETTNHINNAKKK
                     LQSKKCDAIIVNKIDNNKVFGSDHNKVSFIKNNYVKNLKKMSKANVAKELIQFISQLK
                     TN"
BASE COUNT          685 a          365 c          290 g          865 t
ORIGIN      
        1 aattcttctc cgccacctgc aaaaattatt ttttgttttc caatttgaat aagctcataa
       61 gcattaccaa tacaatgcga gcttgtagag caagcagatg taatggaata attaacacct
      121 ttaattttaa agggggtagc tagagtagct gagtttgtac tagacattgt tcttggaacc
      181 ataaacggac caacttttct tgacccacct tctttaccta gttcagaaga tttaagtaaa
      241 ttttgtgtgg agggtcctcc ggaacctaca attatacctg tcatttcatt agacacttca
      301 ccatcctcca aaccggaatc tttaattgca tctttcatcg caatataatt ataagcagct
      361 ccgtccccca taaatcttaa tatgcgtcta tctatttgtt ctccaagatt aatgtcaggc
      421 tttccgtgca ctaaacttct aaaagaaaat tcctcatatt caggtgcttt ggtaattcct
      481 gaattaagat tttttaaatt tttaattact gtttcttgat tatttccaat acaagaaata
      541 attccaagac ccgttactac tactcttctc attttattct atttactagt attgaataat
      601 ccaaccttta tatcttttgc ttcataaatt gttttgccat ctgcttttaa aattccatca
      661 gcaacgccaa gaattaattt tcttaaaatt aatcgtttta aagaaatatg ataggtaact
      721 ttttttacag tttgaagaac ttggccagta aattttacct ctcccactcc cagagccctt
      781 ccttttccta gttggcctaa ccatccaaga taaaaaccaa ctaattgcca catagcgtct
      841 agaccaagac aacctggcat tactggatca tttttaaaat gacaatcaaa aaaccaaagg
      901 ttagattcaa tatcaagttc cgcaataact tctcctttgg caaattcacc acctatttca
      961 ttaatatggg ttattctatc aaacattagc ataggaggaa gaggaagctg agcgttgcct
     1021 ttaccaaaca gtattccttt tccgcattca ataagttttt cgtaattaaa tgaatcctct
     1081 ttcattagaa ttttatagat atagtttatt atgatatttt caattaattc tttaattttt
     1141 gctttattgt aatttacaat caatattctt ttttgtctta atatgactaa tagaaagtaa
     1201 tttgacatct acaagtgtca aaatattatt tttttcatta aatttaagta aaaagttaat
     1261 agctttttgt gcggaaataa ttcctgctag tccagctgta gttcccataa taccaaccgt
     1321 gtcacatctt gctagttctg gttcattgtg attagggaaa atgcattcta aacatagatg
     1381 attatgagta gtattttcaa ataaaatcat ttgaatatca aaacctacaa ctgatatgct
     1441 gagaaggggc aggttttttt ttgtagtata tttatttatc aatctcatgg tttcccagtt
     1501 atctgaggca tcaataataa tggaagaatt tttaagtaaa tgtaagttat cttttgctat
     1561 cttgtggtta taagaattga ttacagcatt agggtttgtt ccaagcagtt ttttcttagc
     1621 aattgtagat tttttctttc caatatcgtg aatagaataa agagtttgac gatttaagtt
     1681 agttttttta atcgtgtcac catcaaatat atttagcttt ttaattccag cagatattaa
     1741 atattgagct aaagggcagc caattcctcc tagcccaata atactaattt cttttttttg
     1801 taattcatta aatttttctt ctttgaaatc ttttagaata agttgacgtg aaaatatttc
     1861 taattcttct tctgttatat tcataatatt taatttgttt tcaattgact aatgaattgg
     1921 atcaattctt tggctacatt tgctttagac atttttttta aatttttgac atagttattt
     1981 ttaataaatg aaactttgtt gtgatcagat ccaaaaacct tattattatc aattttattt
     2041 actataattg cgtcacattt tttagattgt aatttttttt tagcgttatt aatatgattt
     2101 gttgtttctg ccgcaaaacc aataacaata ttcggtcgat ttttttttaa tgaagagata
     2161 ttctgcaaaa tatctctatt cttttttaat attatatttt ttaaa
//
LOCUS       DTVV01000265            1084 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1765053, whole genome shotgun sequence.
ACCESSION   DTVV01000265 DTVV01000000
VERSION     DTVV01000265.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1765053"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            428..658
                     /locus_tag="EYO86_03170"
     CDS             428..658
                     /locus_tag="EYO86_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42065.1"
                     /translation="MKEGSKSRRYAIYAAIIIVAYLVRGFIYAVIATIIAILGLATAL
                     FVLFYIFWPEGDIRGNKFLRSLTRLLKKLRKN"
     gene            710..1084
                     /locus_tag="EYO86_03175"
                     /pseudo
     CDS             710..1084
                     /locus_tag="EYO86_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038273.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
BASE COUNT          365 a          174 c          211 g          334 t
ORIGIN      
        1 acttctagga tttgttatgg cgatagaaag aagcctcata aaaggaaagt acagatgata
       61 aaccaaacac taaaaattat ctggattttt ttgaaaactg caatcctgat ggtcgtgacg
      121 gctacattca ttctggctat tctatcattt tcaagatatt gggattattt caccttgaca
      181 caactaagca cagcctatct tttagtatgg gcaataatta tatcggccat gaaattatat
      241 tacaaaaatg acaacagcag gtggataata ttcggcctcc agtttttcgc aatattgtta
      301 gcggtctatt ttagttataa cgcatttact atgaaagtgt aaaataatca attcgacgtg
      361 tttttgtagt atttgtgtag ttatagtaaa ggaggagatt gcaatttcta acgatatccg
      421 aaactggttg aaggaaggct ctaaaagccg gcgttacgca atttacgcag cgatcataat
      481 tgtagcgtac ttggtaaggg gctttatcta cgcagtaata gccacaataa tagcaatact
      541 tggattggct acagcactct tcgtgctttt ctacattttc tggccagaag gagatatacg
      601 cggtaacaaa tttctgcgca gccttacaag gctgttgaaa aagcttagga agaattagac
      661 ttcagttttc agtgtgtttt tgtattattt tgacataaat caagacataa tgataaacga
      721 atatacaaaa agtaattgtt cagaaattct ctatgttgta aatgacgctg ccgtaagata
      781 caaaaatatc atccctgata attgttggca cgaaccctac atgtcagaaa gagaattatt
      841 aggtgaattt gaagatggag tacaaatgtt cggctatctt cataacaata aattaatcgg
      901 agtgatagga gttcaggaaa taaaagatgt tgtcatgata agacacgctt acaccttaac
      961 tgcctaccaa ggtaaaggca ctggaaatgc attgcttgaa tacctattaa ataatcatta
     1021 aaattctcgt ttattggtgg gcacttggca agatgccaaa tggacaattc gattttatga
     1081 aaaa
//
LOCUS       DTVV01000266            1761 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1766664, whole genome shotgun sequence.
ACCESSION   DTVV01000266 DTVV01000000
VERSION     DTVV01000266.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1766664"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="EYO86_03180"
     CDS             complement(<1..714)
                     /locus_tag="EYO86_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIC42066.1"
                     /translation="MSKLKIQNTLENTKEEFIPVDPSNVRIYACGPTVYDYAHIGNAR
                     MAVVNDLLVRLLRTLFPKVTYVSNITDIDDKIIEAAQTADIPFQELTLKFEAIYNEDM
                     AALGVHRPDVQPRATDHIAEMIALTKTLVKLGHAYEKKGHVLFHVPSFEKYGCLSGRN
                     RDEQIVGSRVEVAPFKKDPADFILWKPSPDPLPGWDSPWGFGRPGWHLECSAMSEKTL
                     GLPFDIHSGGMDLIFPHHEN"
     gene            complement(811..1200)
                     /locus_tag="EYO86_03185"
     CDS             complement(811..1200)
                     /locus_tag="EYO86_03185"
                     /inference="COORDINATES: protein motif:HMM:PF09912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HIC42067.1"
                     /translation="MILLFSSAHLLAHGTIMIEGVEHSEGFIDVKIYENEDSFLKEEM
                     ASEVIRKKATQGKTVIPLSKIHEGQIAIVIYHDEDSNGKLKTGFFWRPKEGFAFSNNY
                     SPKGPPKFSKAAINLIHGTPVTIKLNY"
     gene            1264..>1761
                     /locus_tag="EYO86_03190"
     CDS             1264..>1761
                     /locus_tag="EYO86_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HIC42068.1"
                     /translation="MNNKEYLECLFNSNKPLVIYKVKNGFNVYTDLNAKIKLTSKNVK
                     NFFEKTIYSKNPRNNFFDGYVGFLNYELLCQLINVKIPKQQSNGFHQNIFYKPETLIK
                     IRKNIQILSTIKNYKENNKLTPSRKKFFHEKKFKVNLTLAQYTKLFRNFSQKIREGKT
                     YQIKIC"
BASE COUNT          612 a          303 c          281 g          565 t
ORIGIN      
        1 attttcatga tgaggaaaaa ttaaatccat accaccacta tgtatatcaa aaggaaggcc
       61 caatgttttt tctgacattg ctgaacattc taagtgccac cccggtcttc cgaatcccca
      121 cggtgaatcc catccaggga ggggatcagg gctaggcttc cacaaaataa aatcagcggg
      181 gtcttttttg aaaggcgcaa cctctacacg acttccaaca atttgttcat cacgattgcg
      241 gccagataga caaccatatt tttcaaaaga aggaacatgg aatagtacat gacctttttt
      301 ttcataagcg tgccccaatt taacgagtgt ttttgtaaga gcaatcattt cagctatatg
      361 atctgtagcg cgtggctgta catcgggacg atgaacacca agtgccgcca tatcttcatt
      421 gtaaatggct tcaaatttaa gtgttaattc ttgaaatggt atgtcggcag tttgcgctgc
      481 ttcaatgatt ttatcgtcaa tatcagttat attggataca taagtaacct ttgggaaaag
      541 cgttcgcaac agtcgcacca gtaaatcgtt aacaactgcc atgcgggcat ttccaatgtg
      601 cgcatagtca taaaccgtgg gtccacaagc gtaaatgcga acattcgaag ggtcaacggg
      661 aataaattct tctttagtgt tttctaaagt gttttgaatt tttagctttg acataataat
      721 tgtttgctac cttatattat ccagaaaggt gttttgaact ttaaaattaa aaaaaaggct
      781 tataaaatta aataattgaa tcaaaataca ttagtaattt aacttaatag taacaggtgt
      841 gccatgaatt aggtttatgg cagctttgga aaatttaggt ggtcccttag ggctgtaatt
      901 attactaaaa gcaaaaccct cttttggacg ccaaaaaaaa cctgttttca atttgccatt
      961 cgaatcttca tcatgataga ttactatggc tatttgtcct tcgtgtattt ttgaaagagg
     1021 tataacagtt ttaccttgtg ttgctttctt acgaataact tcggaggcca tttcttcttt
     1081 taagaatgaa tcttcatttt catatatttt tacatctata aatccctctg aatgttcgac
     1141 accctctatc attatggttc catgggctaa aagatgtgca gagctaaata gcaatatcat
     1201 tagaaaaata atgcgcacaa aaaacctttc ctttttttac ttaggctata ttagtcaacc
     1261 ttgttgaata ataaagaata tttagagtgt ttatttaact ccaataagcc cctggttatt
     1321 tataaggtta agaatgggtt taacgtatat actgatttaa atgcaaagat taaactaacg
     1381 tcaaaaaatg taaaaaattt ttttgaaaaa acgatatatt ccaaaaatcc aagaaataat
     1441 ttttttgacg gatatgttgg atttttgaac tatgaacttt tatgccaact tatcaatgtt
     1501 aaaattccca aacaacaatc taacggtttc catcaaaaca tattttataa acctgaaaca
     1561 ttaataaaaa ttagaaaaaa catacaaatt ttaagtacca ttaaaaatta caaggaaaat
     1621 aacaaactaa ccccttcacg taaaaaattt ttccatgaaa aaaaatttaa agttaattta
     1681 acactagcgc aatatacgaa gttgttcaga aatttttcac aaaaaattcg agaaggtaaa
     1741 acatatcaaa taaaaatatg c
//
LOCUS       DTVV01000267            2198 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1772327, whole genome shotgun sequence.
ACCESSION   DTVV01000267 DTVV01000000
VERSION     DTVV01000267.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1772327"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            2..547
                     /locus_tag="EYO86_03195"
     CDS             2..547
                     /locus_tag="EYO86_03195"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC42069.1"
                     /translation="MAPFIFIAIFIGGINDASFGALFPAFMINEFFSDKEIGYLFFIG
                     LFAGVISQPFIGALADKINKRNFIILLLSMHLVWPILLNNTSIIWIAVIIYGIASVSL
                     YTVTLAYLGERVNVAELSIATSVFIIVFESGEFFGPIIVGSSMDYFGNIGFIYSLISF
                     TFLSLLFGLIRTVYIKNKNGI"
     gene            537..986
                     /locus_tag="EYO86_03200"
     CDS             537..986
                     /locus_tag="EYO86_03200"
                     /inference="COORDINATES: protein motif:HMM:PF13673.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIC42070.1"
                     /translation="MEFEIIKANVNHIKQVGELFDLYRQFYKYISNINESTDYISERM
                     KNNESIIFIATNKQKEVMGFVQLYETFGSLHLGKIIILYDLFVKKEFRKYGIGIKLMK
                     KSEEYAKSIGAKGIELSTAKDNLTAQSLYEKIGYIRDNEFYSYSFEL"
     gene            993..1550
                     /locus_tag="EYO86_03205"
     CDS             993..1550
                     /locus_tag="EYO86_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIC42071.1"
                     /translation="MKTVKFTQMSEGDKEDYELLANFEDEFIEGTAERVIRVLKGLDS
                     SLGGYKVSRLEHSLQAASRAMRENAEEEMVVAALLHDIGDEIAPLNHSELAASVLKPF
                     VSEKTRWIVEKHGLFQTYYYNHHYGKDRNLRDQFKGHEFYEDTINFCHRWDQSSFDPN
                     YDTISLDDFIPLVGKIFNRVPYRNL"
     gene            complement(1551..2018)
                     /locus_tag="EYO86_03210"
     CDS             complement(1551..2018)
                     /locus_tag="EYO86_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HIC42072.1"
                     /translation="MSIFLKTGRVKSEWADYNGHMNLAFYIYLFDGAWETLLENFNIG
                     EESAINQKRTTFAVESHTTYDMEVKVGDEVDLNLLFLDRDKKRLVYKLQMIQKEKKYL
                     ASTTEVCSLYVDLDKRKVAEFEDEKLALIDKFIKENAPQFEPGELHLIKKLKK"
     gene            2059..>2198
                     /locus_tag="EYO86_03215"
     CDS             2059..>2198
                     /locus_tag="EYO86_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_105263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HIC42073.1"
                     /translation="MNNNVIISCAVTGAGDTAGKHPDLPITPKQIAEAAIEAAKAGAA
                     VA"
BASE COUNT          735 a          343 c          371 g          749 t
ORIGIN      
        1 aatagcccct tttattttca tagcaatttt tattggagga attaatgatg ctagttttgg
       61 agcactattc ccagctttca tgattaatga atttttttca gataaggaaa ttggttattt
      121 attttttata ggactgtttg ccggtgtaat ctctcaacct tttattggag ccttagcgga
      181 caaaataaat aagagaaatt ttatcatctt gctgttatct atgcatttag tttggccaat
      241 tttgttaaat aatacttcaa ttatatggat cgctgtaata atttatggaa ttgcaagtgt
      301 tagtttatat actgtaactt tagcttatct tggagagcgc gtgaatgtag ctgaactttc
      361 catagctact tctgtattta taattgtatt tgaatcaggt gaattttttg gaccaattat
      421 tgttggttca tctatggatt attttggcaa cataggcttt atttattcat tgatttcatt
      481 tacattttta tcattattgt ttggactaat aagaaccgtg tatataaaaa ataaaaatgg
      541 aatttgaaat tataaaagca aacgtcaatc acattaagca agtaggagaa ttatttgatc
      601 tgtacaggca gttttataaa tatatttcga atattaatga atcaactgat tatatcagcg
      661 agcgcatgaa aaataatgaa tcaataattt ttattgcgac aaacaaacaa aaagaggtaa
      721 tgggctttgt gcaattgtac gaaacatttg gctcattaca tttaggaaaa attatcatct
      781 tatacgacct ctttgtcaaa aaggaattca ggaaatatgg aattggcatt aaattaatga
      841 aaaaatcaga agaatatgcc aaatctattg gcgcaaaagg aattgaactg tctactgcaa
      901 aagataattt gaccgcacaa tcattgtatg aaaaaattgg ttatatcaga gacaatgagt
      961 tttattccta tagttttgag ttataaataa gtatgaaaac agtaaaattc acacaaatga
     1021 gtgaaggtga caaggaggat tatgaacttc ttgctaattt tgaagatgaa ttcatagaag
     1081 gcactgccga aagagtcata cgtgtgctta aaggattaga ttcttcattg ggcggttata
     1141 aagtatctag acttgaacat tccctgcaag cagcttcaag agcaatgaga gagaatgcag
     1201 aagaagaaat ggtagtagct gcattattgc atgatatcgg tgatgaaatt gcccccttaa
     1261 atcactctga gcttgctgct tcggtattaa aaccttttgt aagtgaaaaa acccgctgga
     1321 ttgtcgaaaa gcacggttta tttcagacct attactacaa tcaccattat ggcaaagatc
     1381 gcaatcttcg tgaccaattc aaaggacatg aattttatga agatacgata aatttttgtc
     1441 acagatggga tcagtcttct tttgatccca attatgatac aatctcactt gatgatttta
     1501 ttccattagt tggaaaaatt tttaacagag ttccttatcg gaatttataa ttattttttt
     1561 aattttttaa ttaaatgcaa ttcaccaggt tcaaactgcg gtgcattttc tttaataaat
     1621 ttatcaatca aggccaattt ctcatcttca aattcggcta cttttctctt atctaaatca
     1681 acataaagag agcagacttc agtagttgat gctaaatatt ttttctcttt ctgtatcatt
     1741 tgtaatttgt acactaagcg ttttttatcg cgatcaagaa aaagtaaatt tagatctact
     1801 tcatcgccaa cttttacttc catgtcataa gtggtatgtg actcaacggc aaaagtagtt
     1861 cttttttgat taatagctga ctcttctcca atattaaaat tctccaacaa agtttcccag
     1921 gcgccatcga ataaataaat ataaaaagct aagttcatgt gaccattata atctgcccac
     1981 tcagatttca ctcttccagt tttcaaaaaa atactcactt gttcccctat taaaaataag
     2041 atactacaaa aaacaaaaat gaacaataac gtaataattt cttgtgccgt tactggcgct
     2101 ggtgatactg cgggtaaaca tccagactta ccgattactc caaaacagat tgctgaggct
     2161 gctatagaag cagctaaggc tggtgcggcg gtggcgca
//
LOCUS       DTVV01000268            1407 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1774188, whole genome shotgun sequence.
ACCESSION   DTVV01000268 DTVV01000000
VERSION     DTVV01000268.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1774188"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="EYO86_03220"
     CDS             complement(<1..415)
                     /locus_tag="EYO86_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42074.1"
                     /translation="MSIQETYYQQQNKFLFSNSKLFGNLRKDLLKKFELSSKDNKNNE
                     SLKHLDRNILKFSYKYNNESNKINFINHDENKINIDITDGKISQVENQHKETLHINNI
                     DSKDTSVEDRFLDFQKLFNEDYVVHLNSLMLNSGYE"
     gene            complement(421..1158)
                     /gene="sufC"
                     /locus_tag="EYO86_03225"
     CDS             complement(421..1158)
                     /gene="sufC"
                     /locus_tag="EYO86_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIC42075.1"
                     /translation="MFLSIKNLTVSIENKRILNNFSLNMNEGEVHSIMGPNGSGKSTL
                     AHVLAGKEDYEILEGEIIFKDKNLLDLNIEERSQLGLFLAFQYPVEIPGVNITPFLKS
                     SLNAKLKLEGKPEIDALKFAKELKTLASELGIPLEMLSRSVNEGFSGGEKKRYEILQM
                     SILKPSLSILDETDSGLDVDALKTVMEGVNKIKNDNSSFLIITHYKKLLDYITPDFVH
                     VIKDGTIVKTGDKSIAQQIETEGYKNL"
     gene            complement(1158..>1407)
                     /locus_tag="EYO86_03230"
     CDS             complement(1158..>1407)
                     /locus_tag="EYO86_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIC42076.1"
                     /translation="CGAHTIPYIDSKNSSAVVEHEASTSKINEDQLYYCRQRGISHED
                     AVSLIVNGFCKQVLQELPMEFAVEAQKLVSISLEGSVG"
BASE COUNT          448 a          237 c          181 g          541 t
ORIGIN      
        1 actcgtatcc cgaattcaac atgagagaat ttagatgaac tacatagtct tcattaaata
       61 atttttgaaa atctaaaaac ctatcttcta ctgaagtatc tttgctatca atattattaa
      121 tgtgtaaggt ttccttgtgt tggttttcaa cttgagaaat tttaccatca gtaatatcta
      181 tattaatttt attttcatca tgattaataa aatttatttt attcgattca ttgttgtact
      241 tataagaaaa ttttagaata ttgcggtcta agtgttttag gctttcatta tttttattat
      301 ctttggaaga caattcaaac tttttaagca ggtcttttct taaattacca aataatttag
      361 aattactaaa cagaaattta ttttgctgct ggtaataagt ttcttgaata ctcatttttt
      421 ttataaattt ttataacctt ctgtctcaat ttgttgagca atggatttat ctcccgtctt
      481 aacaattgtg ccatctttta ttacatgaac aaaatctgga gttatatagt ccaagagttt
      541 tttataatgt gtgattatta aaaatgaact attatcattt ttaattttat ttaccccttc
      601 cataacagtt tttaatgcat ccacatcaag tcctgaatct gtttcatcca aaatagataa
      661 agaaggtttt aaaatgctca tttgcaaaat ttcataacgc tttttttccc ctccagaaaa
      721 accttcattc actgagcgtg agagcatttc taggggaata cctaattcac ttgcgagtgt
      781 ttttagttcc ttagcaaact ttaaggcatc tatttctggc tttccttcta attttaattt
      841 tgcatttaaa gaggatttta aaaagggggt aatatttacg cctggaattt ccactggata
      901 ttgaaaagct aaaaataaac caagttgtga tctttcttct atatttaaat ccaaaagatt
      961 tttatcctta aaaataattt caccttccaa aatttcataa tcctctttcc cggccagaac
     1021 atgggccaaa gtacttttgc ctgatccatt gggacccata attgaatgaa cctcaccttc
     1081 gttcatattc aatgaaaaat tgtttaatat tcttttattt tcaattgata ctgtaagatt
     1141 ttttattgac agaaacacta accgacgcta ccttctaaag aaatagaaac taatttttgc
     1201 gcttcaacag caaattccat cgggagctct tgtaaaactt gcttgcaaaa tccattcaca
     1261 attaatgaaa cagcatcttc atggcttatt cctctttgac gacaataata aagttgatct
     1321 tcattaattt ttgaggttga tgcttcatgt tccacaactg cactactatt ctttgaatca
     1381 atataaggaa tagtatgagc gccacat
//
LOCUS       DTVV01000269            1022 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1775682, whole genome shotgun sequence.
ACCESSION   DTVV01000269 DTVV01000000
VERSION     DTVV01000269.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1775682"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1022)
                     /locus_tag="EYO86_03235"
     CDS             complement(<1..>1022)
                     /locus_tag="EYO86_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIC42077.1"
                     /translation="VDVHKGDFIALIGKNGVGKSTLMRIISDKQDLDQGELWKQPGLQ
                     TSYFSQHFDLKESNTVERELANVLKSKDEQYKIDIFCKNLVLDKFAAIRSLSGGQKRR
                     VGLIKALIGESDIVLLDEPTNHLDLECIIWLENYLKSYAGSIICVSHDRTFLSNFTNK
                     VFWLDRGNLRVSPSGFKNFDRWSVELFEQEARQLKNRKQFVNIEVEWASRGVKARVKR
                     NLKRLERAKQLKEQLEKDESSYRKAISSFKAQVLKSGSDNSKFIAEFHKVSISYDDAA
                     KKVLENISIKIHKNDRIGLLGDNGTGKSTFLKALLGQISCSHGTIKLKRNMCFSYFDQ
                     MRNDLDS"
BASE COUNT          309 a          210 c          148 g          355 t
ORIGIN      
        1 gaatctaagt cattgcgcat ctgatcaaag taagaaaagc acatatttct ttttaactta
       61 atggtgccat gagagcatga aatttggccc aagagcgctt tgagaaaagt tgattttcca
      121 gttccgttat cgcccagtaa gccaatcctg tcgttcttgt gaattttaat tgaaatattt
      181 tctaatactt tcttagcagc atcatcatat gaaatggata ccttatgaaa ttctgcaata
      241 aattttgagt tatctgaacc tgatttcaat acttgagctt taaacgagga aattgctttt
      301 cggtaagaag attcatcttt ttcaagttgt tcttttaatt gttttgcacg ctctaatctt
      361 ttcaaattac gctttaccct tgccttcaca cccctactag cccactcaac ttcaatatta
      421 acaaattgtt ttctattttt taattgtctc gcttcttgct caaacagttc tactgaccag
      481 cgatcaaagt ttttaaaacc tgatggactg acccgtaaat ttccacgatc cagccaaaat
      541 actttattgg tgaaattaga gagaaaagtt ctatcatgac taacgcaaat gattgagcct
      601 gcataggatt taaggtaatt ctcaagccaa atgatacatt ccaagtccaa atgattggtt
      661 ggttcatcca gcaaaacaat atctgactcg ccaatcaacg cctttataag gccaacacgt
      721 cttttttgtc ctccagagag acttctaata gccgcaaatt tgtctaaaac gaggttttta
      781 caaaaaatat caatcttgta ttgctcgtct ttactcttta aaacatttgc caattccctt
      841 tctacagtat ttgattcctt tagatcaaaa tgctgactaa aataacttgt ttgtaatccg
      901 ggttgtttcc aaagttctcc ctgatctaaa tcctgtttat cagaaataat cctcattaaa
      961 gttgatttac ctacgccatt ttttccaatt aaggctataa aatcgccttt gtgaacatca
     1021 ac
//
LOCUS       DTVV01000270            1485 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1783088, whole genome shotgun sequence.
ACCESSION   DTVV01000270 DTVV01000000
VERSION     DTVV01000270.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1783088"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1485)
                     /locus_tag="EYO86_03240"
     CDS             complement(<1..>1485)
                     /locus_tag="EYO86_03240"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020169447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIC42078.1"
                     /translation="YLTISFVGFIIFLFILFFFNFKVAFGYFLGALLSGVAGFAGMHI
                     SVRSNARTAESARESLSKGLKVAFRAGTVTGMLVAGLSLLSIGIYYSILVYLGIKGRD
                     LIEPLIGLGFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDCAGMAADLFETYVVTIVATMVLASIFFVDHQFLDQIMIYPIAIGGVCILTS
                     IAGTFFVRLGKSQNVMGALYKGFFATAIFSSVSLWFITDWVIGLNEEFLVNQTSFLGK
                     DLFYCGLIGLIITSLLIWITEYYTGTNYRPVQSIAKASETGHGTNVIQGLAISLEATA
                     LPAIVICIGIISTYILAGLFGIAIAVSTMLALVGMIVALDAYGPVTDNAGGIAEMAKL
                     DKSVRKITDQLDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTADLNYFVENNLIKATN
                     IDFSLSNPYVVVGLITGGLLPYLFASMGMTAVGRAASSVVIEVRR"
BASE COUNT          542 a          325 c          193 g          425 t
ORIGIN      
        1 ccttctaact tcaattacaa ctgaactagc tgctctacca acagcagtca ttcccataga
       61 tgcaaaaaga tagggtaaaa gcccaccagt gataagacca acaactacat atggattaga
      121 taaactaaaa tcaatattag tagcttttat taaattattt tcgacaaaat aattcaaatc
      181 agcagtatat gcagcaaata ggactaaagc acccaagcct gccgaaccaa ttgcataacc
      241 tttagttaca gcttttgtgg tgttgccaac tgcatcaagt tgatctgtta tttttcttac
      301 gcttttgtct aattttgcca tttcagcaat tcctcctgca ttatcagtga ctggaccata
      361 tgcatctaaa gcgacaatca ttccaaccaa cgcaagcatg gtagaaacag ctatagcaat
      421 accaaataaa ccagccaaga tatatgtcga tattattcct atacatataa caattgcagg
      481 taatgctgtt gcctccaaac ttattgctaa accttgaatg acatttgttc cgtggcctgt
      541 ttctgaggct tttgcaatac tttgaacagg cctataattt gttccggtat aatactctgt
      601 aatccaaatt agtaaactgg taataattaa tccgattaac ccgcaataaa ataaatcttt
      661 tcctaaaaaa ctagtttggt tgactaaaaa ttcttcattt aaaccaataa cccaatcagt
      721 tataaaccat aagctaactg aggaaaaaat agctgtggca aaaaaacctt tatacaaagc
      781 tcccatcaca ttctgtgatt taccaagcct aacaaaaaaa gtgcctgcta tacttgttaa
      841 aatacaaaca cctccaattg caattggata aatcattatc tgatctaaaa attgatgatc
      901 tacaaaaaaa atcgacgcca gaaccattgt tgcaacaata gtaacaacgt aagtttcaaa
      961 taaatcagct gccattccag cgcaatctcc cacattatct ccgacattat ctgctataac
     1021 tgctggattt ctaggatcgt cttctggaat tcctgcctca actttgccaa ctaaatcagc
     1081 cccaacatca gctccttttg taaaaatacc ccctccaagt cttgcaaaaa ttgatattaa
     1141 agaagctcca aatcctaatc caataagtgg ctctattaaa tccctgccct taattcctaa
     1201 atagactaaa attgagtaat atataccaat tgacaataag gataatccgg caactaacat
     1261 tcctgtaacg gtacctgctc taaaagcaac ttttaatccc ttgcttaaac tttctcttgc
     1321 agattcagct gttcttgcat ttgacctaac tgatatatgc ataccagcaa atccggcaac
     1381 tccagataaa agagctccaa gaaaataacc aaaagctact ttaaaattaa aaaaaaacag
     1441 aataaataaa aaaattatga atccaacaaa agaaattgtt aaata
//
LOCUS       DTVV01000271            1170 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1785228, whole genome shotgun sequence.
ACCESSION   DTVV01000271 DTVV01000000
VERSION     DTVV01000271.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1785228"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1170)
                     /locus_tag="EYO86_03245"
     CDS             complement(<1..>1170)
                     /locus_tag="EYO86_03245"
                     /inference="COORDINATES: protein motif:HMM:PF00275.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIC42079.1"
                     /translation="FVPGDKSISIRYFFLASQAYGLSRAKGILEESGDIFSSIKALTK
                     LGVRIVKKKKTWFVRGMGLSSLKTRNNLSIDCGNSGTLSRFLLSILSTYAHRIRIYGD
                     HTLNKRPMDRIMNPLEKIGAFFQTKKKVNRTLPCYITGSEMPINIKHEEKIGSGQIVG
                     GLILASLNCPGISTIKIQKNKRSRDHTERMLKFAGARIKVKKFKNYNLISIEGQKDFK
                     AFNLSVGGDFSSASFFIVLTLLSKNSQLKIKSVNLNPTRLGALKILKKMGGKITLKNI
                     RSQCGERIGDIIIKSSNLKPINCPASIVPFAIDEMPLIFLAASFSNGVSIFKGCRELE
                     MKESRRLTLMNKMLNQIGIRTKLIDRDSIKIYGNPNLNLNNRTYRIQTAYDHRLSM"
BASE COUNT          349 a          209 c          183 g          429 t
ORIGIN      
        1 catgcttaat ctatggtcat aagctgtctg tattctgtaa gttctattat ttaaatttaa
       61 attaggattg ccatatattt ttatagagtc gcggtctatt aattttgttc tgattccaat
      121 ttgatttaac attttgttca tcagagttaa tcttcgcgac tctttcattt ccaattctcg
      181 acaccccttg aatatcgata ctccatttga aaaagaagca gccaagaata taagcggcat
      241 ctcgtctata gcaaaaggaa caatagaagc tgggcaatta attggtttta aattactact
      301 ttttattatt atatccccaa ttctttctcc acattgggat cttatatttt taagagtgat
      361 tttgcccccc atctttttca aaatttttaa cgctccgagc cgtgtaggat ttaagtttac
      421 agatttgatt ttaagttggg agttcttgct caacaaagtc agcactataa aaaatgaggc
      481 tgaagaaaaa tcgccaccca cacttaaatt aaatgcttta aaatcttttt gtccttcaat
      541 agaaattaga ttgtaatttt taaatttttt aactttaatt ctagctcctg caaactttaa
      601 cattctttcg gtgtggtctc tagatcgctt gtttttttga attttaattg ttgagattcc
      661 tgggcagtta agcgaagcaa gaatgagtcc gcctacaatt tgaccactac caattttttc
      721 ttcatgtttg atgttaatcg gcatttcgct tccggttata tagcaaggga gagttctatt
      781 tacttttttt ttagtttgaa aaaaagcacc tatcttttct aagggattca ttatcctatc
      841 catcggtctc ttgttaaggg tatgatcacc atatatccga atcctatgcg cataagtgga
      901 taaaattgat aataaaaatc tactgagagt acccgaatta ccacagtcta ttgaaagatt
      961 atttctagtt tttaaagagc ttaagcccat gccccgaaca aaccatgtct tttttttttt
     1021 aactatgcgg actccaagtt tagttagtgc ttttatagat gaaaatatat cacctgattc
     1081 ttcaagaata ccctttgcgc gacttagacc gtaggcctgt gaagctaaaa agaaatatct
     1141 tattgaaatg gatttatcac caggaacaaa
//
LOCUS       DTVV01000272            1118 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1785580, whole genome shotgun sequence.
ACCESSION   DTVV01000272 DTVV01000000
VERSION     DTVV01000272.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1785580"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="EYO86_03250"
     CDS             complement(<1..306)
                     /locus_tag="EYO86_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIC42080.1"
                     /translation="MKILDLTLTISGKIPTFPGSPKPNFIQWENLKDDGYNLELLFLS
                     SHTGTHLDAPYHFLENGSKIHEISLTRLVREAVLIMSRKKANQSITKSDIQKFEKKCG
                     "
     gene            360..623
                     /locus_tag="EYO86_03255"
     CDS             360..623
                     /locus_tag="EYO86_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIC42081.1"
                     /translation="MPTAYVLINSDLGTDESIISKIKEVLKDEKDVEFTTQGVYGIYD
                     IVLKISSDNTDVLRNIITYKIRKINKVQSTLTMMVVEEQEKSD"
     gene            784..>1118
                     /locus_tag="EYO86_03260"
     CDS             784..>1118
                     /locus_tag="EYO86_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42082.1"
                     /translation="MSKWDAPVFYFDFDLLYSGYVTAEEIPLPKNLTILSPDSDNLFE
                     NLKSVIDKTSKTKSLIVLDSLNGFLNLLEGKSDAVRLVNSFVMLLVSSAKDVKSCVIV
                     GSLSKLNDE"
BASE COUNT          378 a          179 c          195 g          366 t
ORIGIN      
        1 tccacatttt ttttcaaatt tttggatatc actttttgtt atagattgat tggctttttt
       61 cctagacatg atcaaaacag cctcgcgaac tagtcttgta agactgattt cgtgaatttt
      121 tgatccattt tctagaaagt ggtacggtgc atccaaatgc gttcctgtat gggaactcaa
      181 aaacaaaagc tccaaattgt agccatcgtc tttgaggttt tcccactgga tgaaatttgg
      241 ctttggcgag cctggaaaag ttggaatctt acctgagatt gttaatgtaa gatctaagat
      301 tttcataaca acaaaatctc ttaatcaaac gttaaataac attttaacta gtttggtatg
      361 tgcctactgc gtatgtactc ataaattctg atctgggtac tgatgaatca atcatttcaa
      421 aaattaaaga agttttaaaa gatgaaaaag atgttgagtt tacaacccag ggggtttatg
      481 gaatatatga tattgtatta aaaataagct ctgataatac ggatgttttg aggaatatta
      541 taacctacaa aattagaaag ataaacaaag tacaatcgac actaaccatg atggttgtag
      601 aagaacagga aaaatcagac tgagacccat attatatttg ataacgtgtt aatcacactg
      661 tgaaaaaatt tgtcgccaaa accttccaaa aatattaaac tcgagagcct gctcgaaaaa
      721 aacacactga atgtggtttt ctacaacaac tctttcgtaa aggctggttt ttttgcaaaa
      781 ataatgtcaa aatgggatgc acctgttttt tattttgatt tcgatttact ttattctgga
      841 tatgtaacag ctgaggagat acctctgccc aaaaacctaa ccatactttc tccggacagt
      901 gataatttat ttgaaaattt aaaatcagtt attgataaaa catcaaaaac aaaatctttg
      961 atcgttttgg attcattaaa tggattcctt aatctgcttg aaggcaaaag tgatgcggtc
     1021 aggctagtca actcatttgt tatgctgctt gtttcttccg caaaagacgt aaaatcctgc
     1081 gtgattgttg gaagcttatc aaaattaaac gatgagaa
//
LOCUS       DTVV01000273            1088 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1793488, whole genome shotgun sequence.
ACCESSION   DTVV01000273 DTVV01000000
VERSION     DTVV01000273.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1793488"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="EYO86_03265"
     CDS             <1..1023
                     /locus_tag="EYO86_03265"
                     /inference="COORDINATES: protein motif:HMM:PF01266.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC42083.1"
                     /translation="RDGPLFLNFPGVLRILPWLLKYLSYSSEVKVNYISDHLAPLLSN
                     SFEEHKKLAEGTDALKWIVNSPFLFIYKNREDYIRETLTWNIRKKHGFKPIEIGKDEL
                     SALVPGLSEEYTFAIKINNQGYISNSKKYLNDLIEGFKKLGGKIIEDEVVDISSDQKT
                     IIIKGKKEEMFSDSVVIAAGVFSDKLSKKFGANVPLQSERGYHLELRETNIKLNYPLM
                     NSSLKLAITPRPNGIRFAGLVEFGSLYSEPNKNAFDLLMRNAKSMFSGITYQEKKEWS
                     GHRPATIDSLPLIGKSSKDEKVFFAYGHHHIGLTAGPKTGELVSKCILSDNDQIDLNH
                     YKPDRF"
BASE COUNT          440 a          143 c          171 g          334 t
ORIGIN      
        1 agagatggac cattattttt aaatttccca ggagtattaa gaattttacc gtggctgtta
       61 aaatacttat cttattcatc agaagttaaa gttaattata tttctgatca tcttgctcca
      121 ttattatcca atagctttga agaacataaa aaattagcag aaggaacaga cgcacttaaa
      181 tggatagtaa actctccttt tttatttatt tataaaaata gagaagatta cattagggaa
      241 acattaactt ggaatataag aaaaaaacat ggtttcaaac ctatagaaat aggaaaagat
      301 gaactctcag cattagtacc aggactatcc gaggaataca cttttgcaat taaaataaat
      361 aaccaaggat atatctcaaa tagtaaaaaa tatttaaatg atttaattga agggtttaaa
      421 aaacttggtg gaaaaataat agaagatgaa gtagttgata tttcatcaga tcaaaaaaca
      481 attattataa aaggaaaaaa agaagaaatg ttttcagata gcgttgtaat tgcagcaggt
      541 gttttttcag acaaattatc taaaaagttt ggagcaaatg ttcctttaca atctgaaaga
      601 ggttatcatt tggaattaag agaaacaaat attaaactaa actatccatt aatgaatagt
      661 tcgttaaaac ttgctatcac ccctagacct aatggaatta gatttgctgg cttagtagaa
      721 tttggaagtt tatattcaga accaaataaa aatgcttttg atttgcttat gagaaatgca
      781 aaatcaatgt tttcaggaat cacatatcaa gaaaaaaaag agtggagcgg acacagacca
      841 gcaacaatcg atagcctgcc attaattggt aagtcatcaa aagatgagaa agtttttttt
      901 gcgtatggac atcatcatat tggtttaaca gcaggtccaa aaactggaga attagtttca
      961 aaatgtattt taagtgacaa tgatcaaatt gatttaaacc attacaaacc agatagattc
     1021 taaaataatt tcaactaaaa gttgttaaac ttctgacttg cactaaataa aatttaatgc
     1081 tcaactca
//
LOCUS       DTVV01000274            1892 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1794126, whole genome shotgun sequence.
ACCESSION   DTVV01000274 DTVV01000000
VERSION     DTVV01000274.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1794126"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="EYO86_03270"
     CDS             complement(<1..330)
                     /locus_tag="EYO86_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC42084.1"
                     /translation="MSTEEKQNLPTYQDSNSVEYPQNEIEVSNFIKKFYKSSVPIELI
                     GSGSKRKIGKPLQCSKTLSLTKLNGIIEYLPEELYIKVKACTSIQQIEEELKKNKQQL
                     AFEPIDFG"
     gene            complement(284..1780)
                     /locus_tag="EYO86_03275"
     CDS             complement(284..1780)
                     /locus_tag="EYO86_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC42085.1"
                     /translation="MSKLFMPKIDRKLISKKKDIIADLKKIIKAENVLDHEDETRPFE
                     TDALSAYKQKPMVVIFPENTEEVSKILAYCNQQRIKVVPRGAGTGLSGGALPLADSIL
                     LCLGKFNKIIDIDYKNRCVVAQPGVTNLSITQAVQDKGFYYAPDPSSQIACSIGGNVA
                     ENSGGIHSLKYGTTTNNLFGVEIVLMDGTISRLGGKTFDSEGYDLLGLVTGSEGLLCV
                     ITEVTVKILKKPQTIKAALIGFSSIEDGGRCVSDIIASGIIPSGMEMMDKALIHATDN
                     FVKAGYPRDAESMLIVELDGTKKEVEELIERVNNIAKKNKSSSIKISKNERQRLKFWA
                     GRKAAFPACGDMAPDYYCMDGTIPRRKLAEVLKEIGRLSKKYNLPVANAFHAGDGNLH
                     PLIMYDANEKNSLERTEKFGADILKYCVKVGGVLTGEHGVGVEKRELMCEMFNNNDIQ
                     QQIKIKKALDSSSLLNPGKVYPILRKCAEEGRVHVHRGKTKFANIPRF"
BASE COUNT          546 a          360 c          247 g          739 t
ORIGIN      
        1 tccaaaatca ataggttcaa aagctaattg ttgtttattt ttttttaact cttcttctat
       61 ttgttgtatt gaagtacaag ctttaacttt aatgtaaagt tcttcaggca aatattctat
      121 gattccgttt agttttgtta aacttaaagt ttttgaacat tgaagtggtt ttcctatttt
      181 tctctttgaa ccggaaccaa taagttctat aggaacactt gacttataaa acttttttat
      241 aaaatttgat acttctattt cgttttgagg atattcaaca gaattagaat cttggtatgt
      301 tggcaaattt tgtttttcct ctgtggacat gcactctccc ttcttctgcg cattttctta
      361 aaattggata aacttttcca ggatttaata atgaactaga atctagtgct tttttaattt
      421 ttatttgttg ttgaatatca ttattattaa acatttcgca catcaattcc cttttctcca
      481 ctcctactcc atgctctccg gttaaaaccc caccgacttt tacacaatat tttagtatat
      541 ctgccccaaa tttttctgtt ctttctaaag agtttttttc gttagcatca tacataatta
      601 atgggtgaag attgccatct cctgcatgaa aagcattagc aactggtaaa ttatattttt
      661 tagacaacct tccaatttct tttaatacct cagctagttt ccttcttggg attgtgccat
      721 ccatacaata gtagtctggt gccatatcac cacacgcagg aaatgctgct ttgcgtcctg
      781 cccaaaattt taatctttgc ctttcgtttt tgctaatttt aatacttgag gatttatttt
      841 ttttagcaat gttatttact ctttcaatta attcttctac ttcttttttt gtaccatcta
      901 attctacaat aagcatagat tcagcatctc ttggatatcc cgcttttaca aaattgtcag
      961 ttgcatgaat taatgcttta tccatcattt ccattccaga aggaataatc ccgcttgcaa
     1021 taatatcaga tacacatctt ccgccatcct ctatactaga aaatccgatt aaagctgctt
     1081 taattgtttg aggtttttta agaattttta ctgtaacttc tgtaattaca cataacaatc
     1141 cttcagatcc agtaaccaat ccaagcaaat catatccttc agaatcaaat gtttttcctc
     1201 ccaacctgct tatagttcca tccatcaaaa caatttcaac accaaaaagg ttatttgttg
     1261 ttgttccata ttttagtgaa tgaattccac cagaattttc cgcaacattt cctcctattg
     1321 aacaagcaat ttggcttgat ggatctggag cataataaaa acctttatct tgaacagctt
     1381 gtgttataga taaattcgtt acacctggtt gagcaactac acacctgttc ttatagtcaa
     1441 tatcaataat tttattaaat ttacctaaac ataaaagtat actatcagcc aatggtagag
     1501 ctcctccgga caatccggtg ccagcacctc ttggaacaac tttaattctt tgctggttac
     1561 aatatgcaag tatcttgctc acttcttctg tattttctgg aaaaataacg accataggtt
     1621 tttgcttata tgccgatagt gcatccgttt caaacggtcg tgtttcatct tcatgatcca
     1681 aaacattttc agctttaata atttttttga gatctgcaat tatatccttt ttttttgata
     1741 taagtttccg gtcaattttt ggcatgaata atttactcat ataaatatat tacagcgaaa
     1801 aagcttagtg gtcaaaaaag ttaagatgaa ttatttaagt attttatcta ttttttcgat
     1861 agctttgctt atattttccc ttgaagtagc aa
//
LOCUS       DTVV01000275            1367 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1799387, whole genome shotgun sequence.
ACCESSION   DTVV01000275 DTVV01000000
VERSION     DTVV01000275.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1799387"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="EYO86_03280"
     CDS             <1..260
                     /locus_tag="EYO86_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC42086.1"
                     /translation="TDTWHGMAEGMVVMSPYNAKLMSADLIQEAINMEIAIKDGTLHS
                     FAGPIYNQAGELVVPEGENADDGMLAGMDFYVQGIDDELPQ"
     gene            337..843
                     /locus_tag="EYO86_03285"
     CDS             337..843
                     /locus_tag="EYO86_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIC42087.1"
                     /translation="MSDLFISWEEYHKKTEELAVKVHEDGWEFNQVVCIAKGGMRVGD
                     IFARIFDLPLAILSVESYKGEGLKNKRSTITFSRDLAKTSPNIGSKVLLVDDLTDSGI
                     TLKKSIDWMQHFYGFYLDEPIRTAVLFHKAVSSFKPDYCIDYLEDSPWIHMPFEKYED
                     MSIEDLKK"
     gene            complement(844..>1367)
                     /locus_tag="EYO86_03290"
     CDS             complement(844..>1367)
                     /locus_tag="EYO86_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIC42088.1"
                     /translation="KNAILSAGSAPNGANLQPWHFSIIKDKIIKRKIRLAAEKEEGEF
                     YKHKAPVDWLKALEPLGTDSNKKFLEEAPILIAVFEKKYEIKKGNKIKNYYVKESVGI
                     ATGILISCLHISGLCMLTHTPSPMNFLNKILNRPNNEKPFVLLVVGFPRKNCKVPKFA
                     KQKKSFEEIATIF"
BASE COUNT          453 a          190 c          244 g          480 t
ORIGIN      
        1 caactgatac ttggcatggt atggctgaag gtatggttgt aatgtctcct tataatgcta
       61 aactaatgtc tgctgattta attcaagaag ctataaatat ggaaatagca attaaagatg
      121 gaacattaca ttcctttgct ggtccaatat ataatcaagc tggagaatta gttgtacccg
      181 aaggagaaaa tgctgatgac ggtatgcttg cgggtatgga cttttatgtt caaggtatag
      241 acgacgaact accacaataa atttatttat tttataaccc cctaaatata tagggggtta
      301 tttttaaata tttatatatt agtaggttga aattatatgt cagatttgtt tatttcatgg
      361 gaagagtatc acaaaaaaac tgaagaactt gcagtcaaag tccatgaaga tggttgggaa
      421 tttaatcagg tagtttgtat agcaaaagga ggaatgagag ttggagatat ttttgcaaga
      481 atatttgatt tacctttggc aattttatcg gtagagtcat acaaggggga gggtcttaaa
      541 aataaaagaa gtacaataac tttctctaga gacttagcaa aaactagtcc aaatattgga
      601 tcaaaagttc ttttagtaga tgatctaact gattcaggaa ttacattaaa aaaaagtatt
      661 gattggatgc agcatttcta tggcttttat ttagatgaac cgattagaac tgcagtgtta
      721 tttcataaag ctgtttcttc ttttaaacca gattactgta tagattatct tgaagattcc
      781 ccttggatac acatgccttt tgaaaaatac gaagatatga gtattgaaga tttaaaaaaa
      841 taatcaaaat atagtagcta tttcttcaaa actttttttt tgttttgcaa actttggaac
      901 tttgcaattt tttctaggga atccaacaac caacaaaacg aatggttttt cattattagg
      961 gcgatttaat attttattta aaaaattcat aggacttggc gtatgtgtta acatgcatag
     1021 tcctgaaata tgcaagcatg aaatcaaaat accagtagca ataccaacag attctttaac
     1081 atagtagttt ttaattttat tacctttttt tatctcatat tttttttcaa agacagcaat
     1141 taaaatagga gcttcttcta aaaatttttt atttgaatct gtccccagag gttctagggc
     1201 cttgagccaa tctacaggtg ctttgtgctt gtaaaattct ccttcttctt tttccgcagc
     1261 caatctaatt ttgcgtttaa ttattttatc tttgataata gaaaaatgcc atggctgaag
     1321 attcgctcca ttaggagcgg agcctgcgga aagtattgca tttttta
//
LOCUS       DTVV01000276            1624 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1799687, whole genome shotgun sequence.
ACCESSION   DTVV01000276 DTVV01000000
VERSION     DTVV01000276.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1799687"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="EYO86_03295"
     CDS             <1..1113
                     /locus_tag="EYO86_03295"
                     /inference="COORDINATES: protein motif:HMM:PF04464.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42089.1"
                     /translation="VFYSESSVILYPYVEEIIRELQNRDQKICYLTSSKHDPILKNEN
                     KNIKVFYIGDSEIEKMKFFLRLKAKVLIMTMPDLGSYHIKRSKIFPVHYIYTFHSMNS
                     THMDGTNYVRTSLFRKSTLDQFDSIFCVGKYQVQEIRATEELYNLNKKNLVECGYGLL
                     DKLIRLRSSSAQQNFLSQNNKKKVLIAPSWGKQNLLESVGIELVKILLDAGYHVTIRP
                     HPMTIKKSPKVVKQIKEKFEKNPDFDLDTNVSSLEQLLSSYALITDWSGIGYEYAFVC
                     ERPVIYVEVLKKARNKEYEKIGLEQFEISIRDKIGEIVSIQNIENIPERIEFLYAHIN
                     DFENKIQKIRNDAIFNIGESGKVMANEIIRIINEKA"
     gene            1178..>1624
                     /locus_tag="EYO86_03300"
     CDS             1178..>1624
                     /locus_tag="EYO86_03300"
                     /inference="COORDINATES: protein motif:HMM:PF12804.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIC42090.1"
                     /translation="MRVIILAAGAGLRLGQHTKDVPKALLDLNGKSILERQISLLRQH
                     GINEIFVVTGYQREKHTLKDIEYIFNPRYAETEQLASMMVARTKISDDVLVIFGDIVF
                     DGQILQQILASNDDIAMAIDLNWEKSYNDGLCNSHPLDEPVLINQKK"
BASE COUNT          606 a          216 c          281 g          521 t
ORIGIN      
        1 gttttctatt cagaaagttc agttatcttg tatccatatg ttgaggaaat aattagggag
       61 ctccaaaata gagatcaaaa aatttgttat ttaacctcaa gcaaacatga tcccattctc
      121 aaaaacgaga ataaaaacat caaagttttt tacattggtg actctgaaat tgaaaaaatg
      181 aaattttttt taagattaaa ggcaaaggtg ttaattatga ccatgcctga tttaggaagt
      241 tatcatatta agcgttcaaa aatattccca gttcattaca tttacacttt tcattcaatg
      301 aatagtacgc atatggatgg tactaattac gtcaggactt ctctctttcg aaagagtaca
      361 cttgaccagt ttgatagtat attttgtgta gggaaatacc aagttcaaga aattcgtgcc
      421 actgaagagt tgtataacct taacaaaaaa aatttggttg aatgcggata tgggttatta
      481 gacaaattaa tcagattaag atcttcatct gcacaacaaa attttctgtc acaaaataat
      541 aaaaaaaagg ttctaattgc accttcatgg ggaaaacaaa atttattgga atctgtgggc
      601 atagaattgg taaaaatttt acttgatgct ggttatcatg tcacaattag accacatcct
      661 atgaccatta aaaaatcacc caaggtagta aaacaaatta aggaaaaatt tgagaaaaat
      721 ccagattttg atttagatac aaatgtatcc tctcttgaac aattgttgtc tagttatgca
      781 ctgattacag attggtcagg aattggttat gaatatgcat tcgtttgcga aagacctgtg
      841 atttatgttg aggttctaaa aaaagctcgt aataaagaat atgaaaaaat tgggcttgaa
      901 caatttgaaa ttagtattag agataaaata ggcgaaattg tttctattca aaatatagaa
      961 aacataccag aacgcatcga atttttgtat gctcatatca acgattttga aaataaaatc
     1021 cagaaaattc ggaatgatgc aattttcaat attggagaat ctgggaaagt tatggctaat
     1081 gagattatta gaataattaa tgaaaaagca taagcaaaat agcttgtgat aaatttgttc
     1141 taacattata ttatggccag ttaatgtcat aaattacatg cgtgtgataa ttctagctgc
     1201 tggggcaggt ttacgattag gtcaacacac aaaagatgtt ccaaaagcgc ttttagatct
     1261 aaatgggaaa tctattttgg aaagacaaat ttcattatta agacaacatg gaataaatga
     1321 aatttttgtt gttactggat atcaacgaga aaaacataca ttgaaagata ttgaatatat
     1381 tttcaatcca agatatgctg aaactgaaca acttgctagt atgatggtag caagaacaaa
     1441 aatttctgat gatgttttag tcatttttgg tgatattgta tttgacggtc aaatccttca
     1501 acaaatttta gcctccaatg atgatattgc tatggcgatt gatttgaatt gggaaaaatc
     1561 ctataatgat ggtctgtgca atagccatcc attagatgaa ccagttttga ttaatcagaa
     1621 aaaa
//
LOCUS       DTVV01000277            2298 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1801859, whole genome shotgun sequence.
ACCESSION   DTVV01000277 DTVV01000000
VERSION     DTVV01000277.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1801859"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="EYO86_03305"
     CDS             <1..761
                     /locus_tag="EYO86_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycolate oxidase iron-sulfur subunit"
                     /protein_id="HIC42091.1"
                     /translation="RVALLTGCVQKVISPQINEATIRLLNRHDIEVVVLKGIECCGSL
                     NHHLGKNDLANKTFKKNISIWYDEYLKNGLDAIISNTSGCGTTLKDYGFIFRSDDNFK
                     KKAKKISELTKDIAEYLDNKVKLNFNTKSINEKKYKIAYHSACSMQHGQKIHKEPINL
                     IKKTGNEVLEIFDGHICCGSAGTYNLLQNDIAKKLLKNKIANIEKIKPQFISTGNIGC
                     ITQIANGTKIPILHTVEIIDWYTGGPKPEVLKQL"
     gene            758..1711
                     /locus_tag="EYO86_03310"
     CDS             758..1711
                     /locus_tag="EYO86_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HIC42092.1"
                     /translation="MTRILITRKLMKSSEDYALKIFDVKLNKEDKLLTKDELISKSSD
                     CDGILSFITEKLDAGIISKLSDKVKIISNFAVGFGNIDIDAAKKRNIVITNTPDVLTD
                     ATAEIAMLVLLGAARRAKEGIEWANKKNWEWSIDFLIGKQLTESRLGILGMGRIGRAI
                     ADRARAFGMKIHYYNRSRLDKNLEKDAIYHKSLESLISVSDFFSINCPATKETKHIIN
                     NKTIKHFPDGAVIANSARGDMIDDDAMVKALKSGKIFSLGLDVYNGEPNIHPEYSTLS
                     NVFVLPHLGSATTKTRIAMGNLAVSNIEEFFKTGQCKNKIN"
     gene            1715..>2298
                     /locus_tag="EYO86_03315"
     CDS             1715..>2298
                     /locus_tag="EYO86_03315"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643534.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIC42093.1"
                     /translation="MNSNNKNIVITSAVRTAVGTFKGSLKNMQGHDLGSVVVKGAIEK
                     SKLKMNEVDELIMGQVLTAAAGQNPARQAAIQAGLPIEKPAYIINQVCGSGLRSIAAG
                     YQAIMSNDSNIVVAGGQESMSNAPQAINIRDDQKLEESNLVDTMIKDGLWDAFNDYHM
                     GITAENVATKWQITRKDQDDFALLSQTKAEQAQK"
BASE COUNT          881 a          309 c          434 g          674 t
ORIGIN      
        1 caagggttgc tttattaact ggatgtgtgc aaaaagttat ttctccacaa attaacgaag
       61 caactattag attattaaat agacacgaca ttgaggtcgt tgttttaaaa ggcatagaat
      121 gttgtggttc tctaaaccat catttgggta aaaatgattt ggccaataaa acttttaaaa
      181 aaaatatttc aatttggtat gacgaatatt taaaaaatgg attagatgct ataatttcta
      241 atacatctgg atgtggtaca actttgaagg actatggatt tatatttaga tcagatgata
      301 attttaaaaa aaaagcaaaa aaaatatcag aattaactaa agacatagca gaatatttag
      361 acaataaagt taaacttaat tttaacacta agtctatcaa tgaaaaaaaa tataaaattg
      421 catatcattc tgcttgttca atgcaacatg gacaaaaaat acataaagaa ccgataaatc
      481 ttataaaaaa aacgggcaac gaagtgctcg agatctttga tggtcatatt tgttgtggtt
      541 ctgcgggtac atataattta cttcaaaacg atatagcaaa aaagttatta aaaaataaaa
      601 ttgcaaatat tgaaaaaata aaacctcagt ttatttctac tggaaatatt ggctgtatta
      661 cgcagattgc aaatggtact aaaattccaa ttttgcacac agttgaaatt attgattggt
      721 atacaggcgg tcctaaacct gaggttttaa aacaattatg acgaggatac tgattactag
      781 aaagcttatg aaatcaagcg aagattatgc tttgaaaatt tttgacgtga aattgaataa
      841 agaagataaa ttactaacaa aagatgaatt aatcagtaaa agcagtgatt gtgatggaat
      901 attatctttt attactgaaa aattagatgc tggcattatt tcaaaacttt cggacaaagt
      961 taaaattatt tctaattttg cagttggttt tggaaacatt gatattgatg cggcaaaaaa
     1021 aagaaatatc gttattacaa atactccaga tgttttgaca gacgcaaccg cagaaattgc
     1081 gatgcttgtg cttctgggag ctgccagaag agctaaagaa ggaattgagt gggctaataa
     1141 aaaaaattgg gaatggtcaa ttgatttctt aataggcaag caactaacag aatcaagatt
     1201 gggaatttta ggaatgggta gaattggcag ggcgattgct gacagagcaa gggcatttgg
     1261 aatgaaaatt cattattata acagatctag attggataaa aatttagaga aagacgccat
     1321 ttatcataaa tcacttgaaa gtttgatatc agtttcggat ttcttttcaa ttaattgtcc
     1381 tgctacaaaa gaaacaaaac acattattaa caataaaact ataaaacatt ttccagatgg
     1441 agctgtaatt gcaaatagcg cgagaggaga tatgattgat gatgatgcaa tggttaaagc
     1501 actaaaaagt ggaaaaattt tttctttagg tctagatgtt tataatggcg agcctaatat
     1561 tcatccggaa tattcaactt tatccaatgt ctttgtttta cctcacttag gaagcgctac
     1621 aacaaaaacc aggattgcaa tgggaaatct tgccgtcagt aatattgaag aattttttaa
     1681 aactggacaa tgtaaaaaca aaataaatta agttatgaat tcaaacaata aaaatattgt
     1741 aataacttca gcagtcagaa cagcagttgg tacattcaaa ggatctctga aaaatatgca
     1801 gggtcatgac cttggttctg ttgtggttaa aggtgctata gagaaatcaa aattaaaaat
     1861 gaacgaagtt gatgaattaa taatgggaca agtactaaca gctgctgcgg gacaaaatcc
     1921 ggctagacaa gcagctattc aagccgggtt accaatcgag aagcctgctt acattattaa
     1981 tcaagtttgt ggatcaggat tgagatctat tgctgctggg tatcaagcaa ttatgtctaa
     2041 cgattcaaat attgtggtag caggaggaca agaaagcatg tcgaatgcac cgcaggcaat
     2101 caatattcgt gatgaccaaa agttagaaga atcaaatctt gtagacacaa tgatcaaaga
     2161 tggtctttgg gatgcattta atgactatca tatgggaatt accgctgaga atgttgctac
     2221 caagtggcag ataacaagaa aagatcaaga cgattttgct ttgttatctc aaaccaaggc
     2281 tgaacaagca caaaagga
//
LOCUS       DTVV01000278            1006 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1810070, whole genome shotgun sequence.
ACCESSION   DTVV01000278 DTVV01000000
VERSION     DTVV01000278.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1810070"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            127..741
                     /locus_tag="EYO86_03320"
     CDS             127..741
                     /locus_tag="EYO86_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIC42094.1"
                     /translation="MREIFKKRNRQAIVEEVVKCYGDLFAFSKFKTKNHHDAQDLVSE
                     AVIRFFNSYDKGTSNYKAGTNILGYLKLIVLNLYRDRKRKEKISPNIIPLHREIDGEE
                     VTNFNEYESQKMEKDEESLKDPYALHVDFKKTKKLMDEKLSEVDSEIFYLRFEEKYKY
                     SEIAKKLKIKQGTIGSRINRSKKRIKQELKKINVIKTKENKNED"
     gene            731..>1006
                     /locus_tag="EYO86_03325"
     CDS             731..>1006
                     /locus_tag="EYO86_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42095.1"
                     /translation="MKINDIILDRFLNNDLSRSKINKIKLLIKKDPKLRSRVQELKSF
                     ESAYEKEYKIAKKTKMPKSLYEKISVEQNTKENYFSKKFNNFYKIAAS"
BASE COUNT          485 a           93 c          152 g          276 t
ORIGIN      
        1 aaaaagatag agaaaaaata atttctgaag caaggaaata cacaatacct ttagagtgtt
       61 ggggtaaaaa ttaatgcaat aaaaattcac tactaccgtt atatttaagt agagaacaaa
      121 ttaaaaatga gggaaatatt caagaaaaga aatagacagg caatagtaga ggaagttgtt
      181 aaatgttatg gagatttatt tgcttttagt aagtttaaaa ccaaaaacca tcatgatgca
      241 caagatctag tttcagaagc agttataaga ttttttaatt catatgataa aggaacaagc
      301 aattataaag caggaactaa tatactggga tatttaaaac tcattgtttt aaatttatac
      361 agagatagaa aaagaaaaga aaaaatatcc ccaaacatta tacctcttca tagagaaata
      421 gatggcgaag aggttacaaa ttttaatgaa tatgaatcac aaaaaatgga aaaagatgaa
      481 gaaagtttaa aagatccata tgctttacat gtagatttta aaaaaactaa aaaattaatg
      541 gatgaaaaat tatctgaagt agatagtgaa atattttatc taagatttga agaaaaatat
      601 aaatatagtg aaatagcaaa aaaactcaaa attaaacaag gaacaatagg aagcaggata
      661 aacaggagca aaaaaagaat taagcaagaa ttaaagaaaa ttaacgttat taaaactaag
      721 gaaaataaga atgaagatta atgatattat actagacagg tttcttaata atgatctttc
      781 cagatcaaaa ataaataaga ttaaattatt aataaaaaaa gatccgaaat taagatctag
      841 ggttcaagaa cttaaaagtt ttgagtctgc ttatgaaaaa gaatataaaa ttgctaagaa
      901 aacaaaaatg cctaaaagtt tatacgaaaa aatttctgtt gaacaaaata ctaaggaaaa
      961 ctatttttca aaaaaattta ataattttta taaaatagct gctagt
//
LOCUS       DTVV01000279            1540 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1816297, whole genome shotgun sequence.
ACCESSION   DTVV01000279 DTVV01000000
VERSION     DTVV01000279.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1816297"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="EYO86_03330"
     CDS             <1..108
                     /locus_tag="EYO86_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIC42096.1"
                     /translation="NDLIEKIKKFSENFHIYTFGGLKETNKWLKENNYV"
     gene            101..1459
                     /locus_tag="EYO86_03335"
     CDS             101..1459
                     /locus_tag="EYO86_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIC42097.1"
                     /translation="MSNEFDYSKLKHAASVDQSDRHVPYNLRQSGPTKVEMLISTRVR
                     KSPYWHLSMKAGCWRATIYNRVYHPRGYVKPEDGGAMVEYEAIKKHVTMWNVAVERQI
                     RVKGPDAEKFTDYVITRDATKISPMRARYVILCNYKGGVLNDPILLRISKDEFWFSLS
                     DSDIHFYLQGVNADKRFNVEIDEIDSCPVQIQGPKSKALMKDLIGDQVDMDNIPFYGL
                     AEAKIGGRDCLISQSGFSGEAGYEIYLRNATLYADDMWNAVLEAGKKHNLMVIAPAHH
                     RRIQAGILSWGQDMDGEHNPFQCNLGYQVSLSGKGEWNKKGDYVGKAVLEKMKADLKD
                     GKKPYKLQLVGLELGGKPIEEYAPDFWLISNEAGGEPVGFITSPWYHPEKKQNIAMCY
                     VPFDGTLNANGMPKGKAGTKYKVHLPEKYLDDLGKPVDAVVVDIPFTESYHASTREVV
                     KG"
BASE COUNT          567 a          198 c          306 g          469 t
ORIGIN      
        1 aatgatttaa ttgagaaaat aaaaaaattt tcagaaaact tccacattta tacattcggc
       61 ggcttgaagg aaactaacaa gtggttgaag gagaataact atgtctaatg agtttgacta
      121 tagtaaatta aaacatgctg catcagttga tcaatctgat cgacacgtgc catacaattt
      181 aagacaaagc ggaccaacaa aagttgaaat gctaatttct acaagagtga ggaagtcacc
      241 ttactggcat ctttcaatga aagcaggatg ttggagagca actatttata atcgtgttta
      301 tcatccaaga ggttatgtaa aacctgaaga tggtggtgca atggttgaat atgaagcaat
      361 taaaaaacat gtaacaatgt ggaatgttgc tgttgaaaga caaataagag ttaaaggtcc
      421 agatgcagaa aaatttacag actatgtaat tactagagat gcaacaaaaa tttctccaat
      481 gagagcacgt tatgtaattt tgtgcaatta caaaggtgga gttttaaatg atccaatttt
      541 attgagaatt tcaaaagatg agttttggtt tagtttatct gactctgata ttcattttta
      601 tttacaagga gtaaatgcag acaaaagatt taatgtagaa atcgatgaaa tagatagttg
      661 tcctgttcaa attcaaggtc ctaagtcaaa agctttaatg aaagatttga ttggtgatca
      721 agtagatatg gataatatac cattttacgg tcttgcagaa gccaagattg gtggaagaga
      781 ttgcttaatt tctcaatcag gtttctctgg cgaagctgga tatgaaattt atttaagaaa
      841 cgctacactt tatgctgatg atatgtggaa tgcagtttta gaagctggaa aaaaacataa
      901 tttaatggta attgcacccg cacaccatag aagaattcaa gcaggaattt tatcttgggg
      961 tcaagacatg gatggtgaac acaatccttt ccaatgtaat ttaggttatc aagtttcact
     1021 ttcaggaaaa ggtgaatgga ataaaaaagg ggattatgtt ggtaaagctg tattagaaaa
     1081 aatgaaagct gatttaaaag atggaaaaaa accatataaa cttcaattag ttggactaga
     1141 attaggtgga aagccaattg aagaatatgc acctgatttt tggttgattt caaatgaagc
     1201 tggtggagaa cccgtaggat tcattacttc cccctggtat catccagaaa agaaacaaaa
     1261 tattgcaatg tgttatgtcc cttttgatgg aaccttaaat gcaaatggta tgcctaaagg
     1321 taaagctgga actaaatata aagttcactt accagaaaaa tatttagatg atctaggaaa
     1381 acctgtggat gcagtagtag ttgatattcc atttacagaa tcttaccatg caagcacaag
     1441 agaagttgta aaaggttaaa ttattttttt tagggggaat ttcatattcc cctaattatt
     1501 tgagtattta ttaaaagttt aatatgtagt tttaaaaaaa
//
LOCUS       DTVV01000280            3159 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1816415, whole genome shotgun sequence.
ACCESSION   DTVV01000280 DTVV01000000
VERSION     DTVV01000280.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1816415"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            133..1077
                     /gene="rsmH"
                     /locus_tag="EYO86_03340"
     CDS             133..1077
                     /gene="rsmH"
                     /locus_tag="EYO86_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIC42098.1"
                     /translation="MKNNHIPVMLEEVQSYIPHDKKINVIDATFGGGGYSRAILKKFD
                     VKQLVAIDRDPISKIFARDLNEKYNNFSLVNGCFGNIDQLIVNNSQIKKIKFDVIIFD
                     LGLSSNQLDNRDRGFSFKYDGPLDMVMGSSSTKASDIINSYPEEQLANIIYKFGDERL
                     SRRIAKNIVKSRKIKFIMTTKELAKLIKQSFTTQQIHKSKIHPATKTFQALRIYVNDE
                     LNELSLALNKSINLLSAKGRLIIVSFQSLEDRIVKDFFNHNSGKRWRSSRHYPELVDE
                     GPITFNIITKKPIRPSEREILNNPRSRSAKLRVGEKIH"
     gene            1079..1396
                     /locus_tag="EYO86_03345"
     CDS             1079..1396
                     /locus_tag="EYO86_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42099.1"
                     /translation="MRYFSAISSLIVLNILTVLFLLYLGNVSREIEKQNYTLEKKINF
                     INDQIRINEIEFSLYHNYGYLKKLHKVYFDGEQFESFSNKRLSFVDIQKNNFKNIYTV
                     GTQ"
     gene            1411..3054
                     /locus_tag="EYO86_03350"
     CDS             1411..3054
                     /locus_tag="EYO86_03350"
                     /inference="COORDINATES: protein motif:HMM:PF03717.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIC42100.1"
                     /translation="MSKKFLIFDSDDLKKFHKRVFFSIIVFLGFYFVAIFRIADVMVF
                     ENNVKEITNIKANIERGKIYDRNGQLLATNIHNYSLAANPLKINNKSDLSNQLSAILF
                     LDKEVIEKKLLSQKKFVWLKRNISPKEHQKIIELGEIHLRTLLETKRIYPFQNIGAHV
                     LGYVDIDNKGQAGIERGLDDILNKGENIFLTIDINLQNAVQEELESTIEKFSADSGVS
                     LLIDIKNGEIISLNNFPDFNPNRINLSSMEGRFNRALQANYEMGSTFKPIIVALGIDE
                     NIINKEMMFDVSKPINSIRDYHPFVGSLSVKDIVVQSSNIGAAKIAHKIGKKKQIEFF
                     RKMGFFEKINIQIKEAAQPLGNKNNWGELETMTIGFGHGFAVTPLHIATAYASILNEG
                     RRVIPTILLGANNISSEALVKKETSEYILELLRAVVTETKYTGPRVRIEGYEIGGKTG
                     TAELINNLGQYQKDANLTSFIGIFPISKPQYLVLTIVENPKKIKEENYNITGAAVNAP
                     LVKNIILRMIEILKIPRLPALEILNAAIRINYHRYYVTQ"
BASE COUNT         1228 a          405 c          509 g         1017 t
ORIGIN      
        1 tcgttgggca aggtcattat tttcaaattt gggaaccaaa agcagcaatt gagagacaga
       61 aaaaatctcg tgctaggtta ataaagaaaa agaaatcctt aagttcaatt atttttccga
      121 tccaaaggtc atatgaaaaa taatcatatc cctgtaatgt tagaagaagt ccaatcttac
      181 atccctcatg acaagaaaat caacgtaatt gatgcaactt ttggaggagg agggtactca
      241 cgagcaatat taaagaaatt cgatgttaaa caattggttg caatagacag agatcctata
      301 agcaagatat ttgctagaga tttaaatgaa aaatataata atttttcttt agttaatgga
      361 tgttttggta atattgatca attaattgtt aataattctc aaatcaaaaa aattaaattt
      421 gatgtaataa tatttgactt aggtttatct tccaatcaat tggataacag agatcgggga
      481 ttttcattca agtatgatgg tcctcttgat atggtaatgg gcagttcctc aactaaggct
      541 tcagatataa taaacagtta tcctgaagaa cagttggcta atattattta taagtttggt
      601 gatgagcgat tatcaagaag aattgcaaaa aatattgtta aatcaagaaa aattaaattt
      661 attatgacta ccaaagaatt agcaaaatta ataaaacaat catttacaac tcaacaaatt
      721 cataaatcaa aaattcatcc tgcgactaaa acttttcaag cacttcgaat ttacgttaat
      781 gatgaattaa atgagctatc cttggcctta aacaaatcta taaatttatt aagtgcaaaa
      841 ggtagattga taatagtttc tttccaaagt ctagaagata gaattgtgaa agattttttc
      901 aaccacaata gtggtaagcg atggcgttcc tcccgccact atccggagct agttgatgaa
      961 ggtccaataa cctttaatat aatcaccaaa aaaccaatta gaccatcaga gcgagaaata
     1021 ttaaataatc ctcgctcacg ttcagctaag cttcgggtag gagaaaagat acattgaaat
     1081 gcgatatttt agtgcaatat catcgctgat agttttaaat attctaacag tcctgtttct
     1141 tttgtatttg ggaaatgttt ctcgcgaaat tgaaaaacaa aattatactt tagaaaaaaa
     1201 aatcaatttt attaatgatc aaataagaat taatgaaatt gagtttagct tatatcataa
     1261 ttatggatat cttaaaaaac tacacaaagt atattttgat ggtgaacaat ttgaatcatt
     1321 ttccaataag agattaagct ttgttgatat tcagaaaaat aattttaaaa atatttatac
     1381 tgtgggtacg caataaattt taggaaatta atgtctaaaa aatttcttat atttgatagt
     1441 gatgatttaa aaaaatttca taaaagagtt tttttttcta ttattgtttt tcttggtttt
     1501 tattttgtag caatatttcg aatagctgat gtcatggtat ttgaaaataa tgtaaaagag
     1561 attaccaata ttaaagcaaa tattgaaaga ggaaaaattt acgatagaaa tggtcagtta
     1621 ttagctacaa atatacataa ctattcactt gcagcaaatc cattaaaaat aaataataag
     1681 tctgatttat ctaatcaatt gtcagcaatt ctttttttag ataaagaagt aatagaaaaa
     1741 aaattgttat cacaaaaaaa atttgtttgg ttaaaaagaa acatatcccc aaaagagcat
     1801 caaaaaatta ttgaacttgg tgaaattcat ttacgcactc tattggagac taaaagaata
     1861 tacccttttc aaaatattgg tgcacatgtt ctcggttatg ttgatataga taataaaggg
     1921 caggctggaa ttgaaagagg tttagatgac atattaaata aaggagaaaa tatttttttg
     1981 acaatagata ttaatttaca aaatgcagtt caagaagaat tggaaagcac tattgaaaaa
     2041 ttttcagcag attcaggggt atccttatta attgatataa aaaatggaga aataatttct
     2101 ttaaataact ttcctgattt taatcctaat agaattaatt tatcaagtat ggaaggtcga
     2161 tttaatagag ccttgcaagc taattatgaa atgggatcaa cttttaagcc aattattgtt
     2221 gcattgggaa ttgatgaaaa tataattaac aaagaaatga tgtttgatgt ttctaaacct
     2281 atcaattcaa tacgtgatta tcacccattt gttggatcat taagtgtaaa agatattgtt
     2341 gtacaatcat caaatattgg agctgctaaa attgctcata aaattggtaa aaaaaaacaa
     2401 atagaatttt ttagaaaaat gggttttttt gaaaaaataa acattcagat aaaagaagca
     2461 gcgcagcctc ttggaaataa aaataattgg ggtgaattag agactatgac aataggtttt
     2521 ggtcatggtt ttgctgttac tcctcttcat attgcaactg cttatgcatc aattttgaat
     2581 gaaggaagga gagttatacc aacaatatta ttaggggcta acaatattag ttcagaagcg
     2641 ttagttaaaa aagaaacatc agaatatata ttagaattat taagggctgt tgtaacagag
     2701 acaaaataca ctgggcctag agtgagaatt gaaggttatg aaataggtgg caaaaccggt
     2761 actgctgaat taattaacaa tcttggacaa tatcaaaaag atgcaaacct gacatcattt
     2821 ataggtatat ttcctatatc aaaacctcaa tatttagttt tgacaattgt tgaaaatcct
     2881 aaaaaaatca aagaggaaaa ttataatatt accggagctg ctgtaaacgc tcctcttgta
     2941 aaaaatatta tattaagaat gatagaaata cttaaaatac ctaggcttcc tgctttagaa
     3001 attcttaatg ctgccataag aataaattat catagatatt atgttactca atgatttacc
     3061 aaagtatata gaatgcaaaa aaatacggcg gtataactct gtctgaaact ggcgcgccag
     3121 cggtgtccag tcggaacgga ccgcttccag tcctttaaa
//
LOCUS       DTVV01000281            2229 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1816872, whole genome shotgun sequence.
ACCESSION   DTVV01000281 DTVV01000000
VERSION     DTVV01000281.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1816872"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="EYO86_03355"
     CDS             complement(<1..716)
                     /locus_tag="EYO86_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102231.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIC42101.1"
                     /translation="MKIRAAEISSVIKDQISNFETKADVAEVGTVLSVGDGIARVYGL
                     DQVQAGEMVEFPGGIKGMALNLEMDNVGVSIFGDDTGIKEGDTVKRTGEIVDVPVGKE
                     LLGRVVDGLGNPIDGKGPIKSDDRRRIELKAPGIIPRQSVTEPMQTGIKALDALVPIG
                     RGQRELIIGDRQTGKTAVAIDTILNQKSVNQSDNEKVKLYCIYVAIGQKRSTVAQIVK
                     ILEDNGALEYTIVVSATASEP"
     gene            complement(716..1273)
                     /locus_tag="EYO86_03360"
     CDS             complement(716..1273)
                     /locus_tag="EYO86_03360"
                     /inference="COORDINATES: protein motif:HMM:PRK05758.3-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIC42102.1"
                     /translation="MASQLASGDLIADRYASAIYDLALENKAVDVVLKDLEALKKIIQ
                     GNKELNLLVKSPLIASNDKLEILIKLTKEKSTSEMTTTFLKVISKNKRFASLTSIILQ
                     FININAQKRGEVLAEITSADKLSDKQKDDIKDHLGTILGDKLSLNFKVDKKIIGGLIV
                     KVGSKMIDASLANKINKLKIAMKGV"
     gene            complement(1393..>2229)
                     /gene="priA"
                     /locus_tag="EYO86_03365"
     CDS             complement(1393..>2229)
                     /gene="priA"
                     /locus_tag="EYO86_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIC42103.1"
                     /translation="SWLVMHHNKKRLLCHHCGTIYQIQSTCPQCAAEDSIKLIGPGVE
                     RLAEELKFLFSNKSIGIMSSDNANTPNKIKKIIDDFDNKKIDILVATQIMSKGYHFPN
                     LSFVGVIDADSGLMGGDIRAIERTYNLLQQVSGRAGRSNKMGKAYIQTYFPNQPVIQS
                     LQKRDRKTFVEQSLKDREAFQIPPFGHMTALIISGSSKSKTEIYAGNLSRAHKIENNL
                     SVLGPVEAPIFLLRGQYRFRLLLKGNSRKILNKFTRKIIKLCPPPPTIKLLVDVDPYS
                     FV"
BASE COUNT          646 a          429 c          342 g          812 t
ORIGIN      
        1 ggttctgaag ctgttgcgct tacaacaatg gtatactcca aggcaccgtt atcttccaat
       61 attttaacaa tctgtgccac cgttgatctt ttttgaccaa tagcgacata aatacaatac
      121 aattttactt tttcattatc tgattggttg actgactttt gatttaatat ggtatcaata
      181 gcaactgccg ttttaccagt ttgacgatca ccaatgataa gttcccgttg accacgtcct
      241 atcggaacca aagcatcaag agctttgatc ccagtctgca taggttctgt aacgctttgt
      301 cggggaataa ttcctggtgc cttcaactca attcttcttc tatcatctga tttgattggc
      361 cctttcccat caattggatt tcctaaccca tcaacaactc gtcctaaaag ttcttttcca
      421 actggtacat caacaatctc gcctgtacgt tttacagtgt cgccttcttt aatccctgta
      481 tcatctccaa aaatactaac gccaacatta tccatttcca gattcagtgc cataccttta
      541 atgcctccag gaaattcaac catctctcct gcctgtactt gatctaaccc ataaaccctt
      601 gcaataccat caccgacact tagaacagtt cctacctcag caacatctgc tttcgtttca
      661 aaatttgata tttggtcttt tattacagat gaaatttctg ccgctctaat tttcattaca
      721 cccccttcat tgcgattttg agtttattaa ttttattagc taaagatgcg tcaatcattt
      781 tagagccaac cttgacaatt aatccaccaa taattttttt atctacctta aagttaagtg
      841 acagtttgtc acccaagatt gttcctaaat gatctttaat gtcatctttt tgtttatcag
      901 ataatttatc agcagaagta atttctgcta gaacttcacc tcttttttgg gcattaatgt
      961 ttatgaattg taaaataatt gatgttaaac ttgcaaaacg tttattttta gaaattactt
     1021 ttaaaaaggt ggtggtcatt tcgctagttg atttttcttt tgttaatttt attaaaattt
     1081 ctaatttatc atttgatgca attaaggggc ttttaacaag aagatttaat tctttattcc
     1141 cttgaataat tttttttaat gcttctaaat cttttaacac tacatcaact gccttatttt
     1201 ctaatgcgag atcgtatatc gcagaagcgt atctatctgc tattaagtcc cctgacgcta
     1261 attgtgatgc cattaatttt tccttaatta agatatgtga aaggctatta acacatcaaa
     1321 aaattaaggt aaactgtgag tttatgcggc aaatagtgtt aatttaataa aataattgta
     1381 gctgtgggta aattaaacaa aagagtaggg gtcaacatca accagaagct taattgtggg
     1441 agggggtgga cataatttaa ttattttcct tgtaaactta ttcagtattt ttctgctatt
     1501 tcctttaagt aggagcctaa aacgatattg tccgcgtaat aaaaatatcg gtgcttctac
     1561 tgggcccaaa acacttaaat tgttttcaat tttatgggcc ctagataaat tgcccgcata
     1621 aatttccgtt ttagattttg aggatccgga tataattaat gctgtcatat gtccaaaagg
     1681 ggggatctgg aatgcttcgc ggtctttcag tgactgctct acaaaagttt ttctatctct
     1741 tttttgaaga gattgaataa caggctgatt tggaaagtat gtttgaatat atgctttacc
     1801 cattttattt gatcgccctg ctcgaccgct tacttgttga agtaaattat atgttcgttc
     1861 aatcgctctt atatctcctc ccattaaacc tgaatctgca tcaatcactc ctacaaagga
     1921 taaattagga aagtggtatc ctttggacat aatttgggtt gcgaccaaaa tatctatttt
     1981 tttgttatca aaatcatcaa taattttttt tattttattg ggagtgtttg cgttatcact
     2041 agacataata cctatacttt tattggaaaa taaaaacttt aattcttcag ctaaacgctc
     2101 tactcctggg ccaatcaatt ttattgaatc ctcggcagca cattgtgggc atgttgattg
     2161 aatttgataa atagttccgc aatgatgaca cagcaaccgt tttttattat gatgcatgac
     2221 aagccaaga
//
LOCUS       DTVV01000282            1512 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1817724, whole genome shotgun sequence.
ACCESSION   DTVV01000282 DTVV01000000
VERSION     DTVV01000282.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1817724"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="EYO86_03370"
     CDS             complement(<1..214)
                     /locus_tag="EYO86_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42104.1"
                     /translation="MKRENLIARKKVKNNKHLFKDSKILNFYKKFKSIIFKDIKDKTG
                     SLNLKIVHVYRWDPEKNDNPFSPPLAA"
     gene            complement(177..1133)
                     /gene="ybgF"
                     /locus_tag="EYO86_03375"
     CDS             complement(177..1133)
                     /gene="ybgF"
                     /locus_tag="EYO86_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIC42105.1"
                     /translation="MNRIFSLLIKVLLFFSLISFYSFKVYAEEIETILNQLQEDIKTL
                     EKAVYSQDVKITSSDVELSSHSSDILTKHLLKLSELEEQFQILTNNFEEINFKLDKLS
                     NRITKVQTDNQMRFRDLEKSGLDKSSNTALSELKKLPGSGEAQDLGGVSDSDVAAAEQ
                     IQKTQSIESVGTIVTENTPRIENILPDTTPEEQYKFAISFIKVGDYETAEFALREFVD
                     INSGHKLAGNAQYWYAETFRVRQLYQDAATAYLDGYQKYPKSTKAPVNLLKLGVMLVQ
                     IGEKEQGCSMIIGIKDQYPEANQSVIQKAEYEKRKFNCEKKS"
     gene            complement(1220..>1512)
                     /locus_tag="EYO86_03380"
     CDS             complement(1220..>1512)
                     /locus_tag="EYO86_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020169144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein"
                     /protein_id="HIC42106.1"
                     /translation="TTASRDTLRKQAGWMRAKGKDLTFTIEGHADERGTREYNLALGE
                     RRANVVKDYLLTYGISGGRLSVISYGKERPVNSGSNPLAWSQNRRSVTVKAN"
BASE COUNT          464 a          268 c          205 g          575 t
ORIGIN      
        1 aggccgccaa gggtggggaa aatggattgt catttttttc tggatcccat ctgtaaacat
       61 gaactatctt caaatttaaa gaaccagttt tatctttaat atctttgaaa attatagact
      121 taaatttttt ataaaaattt aggattttag aatctttaaa taagtgttta ttatttttaa
      181 ctttttttct cgcaattaaa ttttctcttt tcatactcag ctttttgaat aacagattgg
      241 tttgcttctg gatattgatc ttttattcct ataatcatag aacatccttg ctctttttcc
      301 ccaatttgta ccaacataac acccaattta agtaagttta ctggtgcttt agtgctcttt
      361 ggatattttt gataaccgtc caaatatgcg gtagccgcat cttgataaag ctgcctaact
      421 ctgaaagttt ctgcatacca atactgagca tttcctgcaa gcttatgccc agaattaata
      481 tctacaaatt ctcttaaagc aaattctgca gtttcataat ctccgacttt aataaaactt
      541 atagcaaatt tatattgttc ttcaggggtg gtgtctggaa gaatattttc aattctggga
      601 gtattttctg taacaattgt acctactgat tcaatagact gggttttttg aatttgttca
      661 gcagccgcaa catcagaatc tgatactcct cccaagtcct gtgcttctcc actgcctggt
      721 aattttttta gttcactaag agcagtatta ctacttttat ctaaaccaga tttttctaaa
      781 tctcgaaaac gcatttgatt gtctgtttga acttttgtaa ttctgtttga tagtttatct
      841 aatttaaaat taatttcttc aaagttattt gttaaaattt gaaactgttc ttcaagttca
      901 gatagtttaa gtaaatgctt agtcaaaata tcactgctat gacttgataa ttccacatcg
      961 ctagaagtaa ttttaacatc ttgactgtaa actgcttttt ccaaagtttt aatatcttcc
     1021 tgaagttggt ttagtattgt ttctatttct tcagcataaa ctttaaaaga gtaaaagcta
     1081 attaaagaaa aaaatagcaa tactttaatt agtaaagaaa aaattctatt cataaatttt
     1141 tatgtttaaa gaaaaataag gcaaaaaaaa gacccacaaa atgtaatttt gtgggtcttc
     1201 ttagaatttt attctagtat taattagctt taacagttac tgatcttctg ttctgagacc
     1261 aagctaacgg attagaacca gaattaactg gcctttcttt cccatagctt ataaccgaca
     1321 atctacctcc tgaaatacca taagtcagta aataatcttt aactacatta gctcttcttt
     1381 cacctagagc caaattatat tctctagttc ctctttcgtc agcatgcccc tcaattgtaa
     1441 acgtaagatc tttacccttt gctctcatcc aacctgcttg cttacgtaaa gtatctctag
     1501 atgctgttgt ta
//
LOCUS       DTVV01000283            1722 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1825769, whole genome shotgun sequence.
ACCESSION   DTVV01000283 DTVV01000000
VERSION     DTVV01000283.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1825769"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
BASE COUNT          552 a          309 c          199 g          662 t
ORIGIN      
        1 atcatatttt tcattataag ggttatttgg caaatgtaga aataccaata gataaaatgc
       61 aatctttctt agaaaaacat cgaaattcat ttgaaaaact agctgctgaa attgtaaaaa
      121 agattcaaaa ttatcagtct aaataaattc gccgggtgat tggtttattc caacatcctt
      181 ttctaaaatt tgtgtctctt taaccaattt ccctttatac gtttttttat gtttaatatc
      241 atcaaactct acatctctca aaagcacatg gcctggtatc cttgataata tctttccaat
      301 tccatttata tcggaacctc tctcaacaat ttgtttttta atcactgctt ttttgtcatt
      361 gtttgtaaaa aatacaacct ttattggttt gtctagtttt tctggaagcc actcaaactc
      421 ttcttccatt acttttttaa aatcataatt aaattcaaat tcgtatcttt gaatataatc
      481 aaaaacattt ttttttctta gcttactaaa ctccttcaat tctaaaagat aatcataata
      541 ctctgaaaat gcatctggta ctttggtttt cattaacact tcagcaattt taaaagcttc
      601 atcatgaatt tcatccatta ggtttagtgc tgcaatagct ctatgtttaa aaagaacatt
      661 cacacccaat tcgcctttat agtatctatc aataacagtt ctgtctgatt ttatgaattt
      721 ctttaatttt tcttttgaat cccaaagttc tgttttagta tcataatcaa aagatgaata
      781 aagactagtt aatttttcag agaatttatg tctattatta tgtaagattg taaggagctc
      841 tgaaggtttt atctcaaaat ttttaagaaa tctgtataat cccattaaaa tcctatcttg
      901 atagaaaagt gcttcagtta aagcaaaaga tctgcactca taatagtcat ctaatgtaag
      961 actttccgtt gttatacata tttcttcgat ctctgcacat aaaatttctt tgccatcaaa
     1021 atcataagct ccatattgtc ctggaagaac tctaaacttt gtatccatct tccatttctg
     1081 cacagaatca tttgaaccaa gttctgatcc aacaagaatc atacactgat acatacaaat
     1141 tgtatccatg cctgcgtcag ctaattttag aactgttttc atgtgttttt ctttagaatc
     1201 tcccggaagc gctaaaatta cttctgaata tgtattatcg ctaattttac ttcctacttt
     1261 cgtcatttca agcaattgat cttcagaaat attattccgc ttaatcttta acaacacatc
     1321 tttatccaat gattgtactg atgcagataa acccatactt ccttttagaa tatcaacaca
     1381 ctctaaaact ctggctttgt tattttttcc tgtacatact tgaatataat atggataatt
     1441 gaatttttct tttactctag cgattgcttt tgaaatctct atgtctctat tatacattcc
     1501 aaaattgaga tcagctaagt ttagaacctt gcctttaaaa tgtgttgcaa tatattctaa
     1561 ctctttttca aatcttgaaa cttcttttga ataaactttt gaacgatctt taataccatc
     1621 aacacaaaat gtgcatgtaa atggacatcc tctattagat tctaatagtg gtgtaagttt
     1681 cgggtcattc agaaattcat aaagatatcc ttctaaataa gg
//
LOCUS       DTVV01000284            1352 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1827363, whole genome shotgun sequence.
ACCESSION   DTVV01000284 DTVV01000000
VERSION     DTVV01000284.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1827363"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(41..1324)
                     /locus_tag="EYO86_03385"
     CDS             complement(41..1324)
                     /locus_tag="EYO86_03385"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC42107.1"
                     /translation="MKGGKSDYEGYRKWVSLVANQLARVILGIKLHECTTSFRAFRVP
                     LFNSLNLSPIKSNGYSFFLECVYELKCVDAEMTEVPIHFKDRFHGESKIPKTEIFRSM
                     YKFASLFLSRFYKKQTDLSNPIEIKSSCYLCNSQCLVEINHGNTINDIVGAQAYKCTS
                     LEHKSKPQVLNCLICDLSFVPQTSYPADIENIYSEVEDPIYLANKESRYKTFQESLAQ
                     ISPYLPDKEGNLIEVGSYCGIFLDTFQKKYPGWDYIGVEPSKWASEYARSQLNINTRT
                     GTLEDNIKELTPDYDVAVAWDVLEHLKDPLAFLLQINSLIKIKGIFCFSTLDIDNWLP
                     KIMGRHWPWLMEMHLFYYKSYTTEQLLGKAGFKIIESEKYRHYVSIHYLFGKIIAILP
                     KWLEKPLDYFSSFLPQKIVIPISFGDIKLYICEKT"
BASE COUNT          423 a          251 c          203 g          475 t
ORIGIN      
        1 accttagtaa atcaaaaatt gttgcattct aaactattaa ttacgtcttt tcacaaatat
       61 ataattttat atcaccaaaa ctaatcggaa tcactatttt ttgcggcaaa aaactactaa
      121 aataatcaag tggtttttct aaccactttg gtaaaatagc aataattttt ccaaacaaat
      181 aatgaataga gacatagtgt ctatattttt ctgattctat tattttaaat ccagcttttc
      241 ctagcaattg ttcagttgta tagcttttgt aataaaatag gtgcatctcc attaaccacg
      301 gccagtgtct acccattatt ttagggagcc aattatcgat atcaagtgtg gaaaagcaaa
      361 aaattccctt tatctttatt aatgaattta tctggagtag aaacgctaat gggtccttca
      421 aatgttcaag tacatcccag gctactgcta catcataatc tggtgtcaat tctttgatat
      481 tgtcttcaag tgtcccagtt cttgtattta tattcagttg actcctggca tattcagagg
      541 cccattttga tggttccaca ccaatgtagt cccaaccagg atattttttt tggaatgtgt
      601 cgagaaaaat cccgcagtaa gatcccactt caataagatt cccttctttg tctggcagat
      661 atggtgaaat ctgtgccaaa gattcttgga atgttttata ccttgattct ttatttgcta
      721 agtaaattgg atcttctact tcagaataaa tattttcaat atccgctggg tagcttgttt
      781 gtggtacaaa acttaagtcg caaatgagac aatttaatac ttgtggtttg gacttgtgct
      841 ccaggctggt acacttgtag gcttgcgctc caacaatgtc attaatagta tttccatgat
      901 taatttcaac taaacactga ctgttgcaca agtaacatga actctttatt tctataggat
      961 tgcttaaatc cgtttgtttt ttgtaaaatc ttgacagaaa caaacttgca aacttgtaca
     1021 tactcctgaa tatttctgtt ttaggaattt ttgattcccc atggaaacga tccttaaagt
     1081 gtattggaac ttccgtcatt tctgcatcaa cacactttaa ttcataaaca cattccagga
     1141 aaaaagaata accatttgat tttattggac ttagatttaa tgaattaaac aaaggaacac
     1201 gaaatgctct aaatgaagtc gtgcattcgt gtaatttaat tcctagaatt actcgtgcaa
     1261 gttgatttgc caccaggcta acccattttc tgtaaccttc ataatcactt tttccaccct
     1321 tcatgtaacg tgaaccaata acaaaatctg aa
//
LOCUS       DTVV01000285            3442 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1830885, whole genome shotgun sequence.
ACCESSION   DTVV01000285 DTVV01000000
VERSION     DTVV01000285.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1830885"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="EYO86_03390"
     CDS             complement(<1..219)
                     /locus_tag="EYO86_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42108.1"
                     /translation="MPKRKDIQKILIIGAGPIVIGQACEFDYSGAQACKSLREEGYEV
                     VLINSNPATIMTDPELANKTYIEPITKEF"
     gene            complement(212..1381)
                     /gene="carA"
                     /locus_tag="EYO86_03395"
     CDS             complement(212..1381)
                     /gene="carA"
                     /locus_tag="EYO86_03395"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIC42109.1"
                     /translation="MKDTNDHPSLKPPSAALILENGKVYWGQGMGAKTSNIGELCFNT
                     SQTGYQEILTDPSYAKQIITFTFPHIGIVGTNNLDVESNKIHASGCILNQTVHHESSW
                     RSDSNLDFFFVQNNTPCITNIDTRQLTRLLAEQGAIKAAIINFGEDKVSLDLLSQEID
                     KWNGLQNLDLASVVTTLDEYKWSENIWQLENNQYTKKNNQSFNITCLDFGIKQNILRN
                     LINSNFAPTVLPSHASFDSIMKTNPDGIFLSNGPGDPFATGEKVIPVINKLIKKEIPI
                     FGICLGHQILSLALGAKTKKMFQGHRGGNHPVKNLETSIVEITAQNHGFEVDRSSLPS
                     NVKETHVSLFDGSNEGIKHVNLPIFSVQYHPEASPGPHDSHYLFDQFHQLIQKNA"
     gene            1515..1973
                     /locus_tag="EYO86_03400"
     CDS             1515..1973
                     /locus_tag="EYO86_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIC42110.1"
                     /translation="MSLRIIIEDKYKNAIKAKNTIEINTFRLIKSAIKDRDISNRSGD
                     NNKGIVDLEILSLLQNLIKQRKDSIDSFKVASRNDLIAVEQGEIDVIRQFLPQQLNEE
                     DTEKIIEKIISENNFYTLKDMGGLMKNLKSSHAGSIDMALAGKIAKSKLS"
     gene            1976..>3442
                     /gene="dnaG"
                     /locus_tag="EYO86_03405"
     CDS             1976..>3442
                     /gene="dnaG"
                     /locus_tag="EYO86_03405"
                     /inference="COORDINATES: protein motif:HMM:PRK05667.2-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIC42111.1"
                     /translation="MSFLKTDLDKIKSKISISSELEKKAKLVQKGRDYWCCCPFHEEK
                     TPSCKINDDQGSFYCFGCGAKGDIFTLYTDLYNYNFVDAVKELSQRAGVNIKFQDAQQ
                     TKQENQVEAILKLTSEWYQNNLEVSEAQKCKLYLEGRNLSDETISQFKLGYSYNASST
                     LYKFLKSKSFNDEDLLKSNVVKLDKNNKIRDYFYKRLIFPILNLQGHIVGFGGRALDD
                     SNPKYINSPESNIFQKRHLLYNLNLAKNTARKKNNILICEGYMDVISLYQNGLQSVVA
                     PLGTALTEDQLQLSWKYSTKPTIMFDGDNAGIRASYKSAVMALPLISAKKFLQFVLLP
                     EGFDPDSFINNVSFDKFVELLKKPQSLAHFIFSQSSKSIKLNSADEKISYDKYLDDLV
                     QTIKDKKTQYFYKNELKSLFFDKIRQSRSSFKGKNITVKKINTSLYNQQILSFIASAI
                     NHVPIRQKILEDLQATDLLNNAHRNLIRDIKSDDNLDLQ"
BASE COUNT         1198 a          495 c          580 g         1169 t
ORIGIN      
        1 aaattctttt gtaattggtt caatataagt tttattagct aattcgggat ctgtcataat
       61 agtagctgga ttagaattaa ttaaaacaac ttcataacct tcttctctaa gcgatttgca
      121 tgcttgtgct cctgaataat caaattcaca agcttgacca attacaattg gcccagcacc
      181 aattattaaa attttttgaa tatcttttct cttaggcatt tttttgaatt aattgatgaa
      241 attgatcaaa caagtaatgg ctgtcatgtg gaccagggct ggcctcagga tggtattgga
      301 cactaaagat tggtaagtta acatgcttta taccttcatt agaaccatca aaaagcgaca
      361 catgagtttc tttgacattg gaaggaaggg aagatcgatc aacttcgaac ccatgatttt
      421 gcgcagtaat ttccacaata gaggtttcta aattttttac tggatggttt cctccgcgat
      481 gaccttgaaa catttttttt gttttagctc ctagtgccaa acttaaaatt tgatgaccca
      541 aacagatacc aaatattgga atttcttttt ttataagttt gttaattaca ggaataactt
      601 tttctcccgt agcaaaagga tctcctggtc catttgataa aaaaattcca tcagggttag
      661 ttttcattat tgaatcaaaa gaagcgtggg aaggtaagac tgtaggtgca aagttggaat
      721 taattaaatt tcgtaaaata ttttgtttaa ttccaaaatc aagacaagta atattgaatg
      781 attgattatt tttttttgta tattgattat tttctagttg ccaaatattt tctgaccact
      841 tgtactcatc cagagttgtg acaacagagg ctaaatcaag attttgtaat ccgttccatt
      901 tatcaatctc ttgtgataag aggtctaagc taactttatc ttctccaaaa ttaataatgg
      961 ctgcctttat tgctccttgt tcggcaagta aacgagttag ttgacgtgta tctatattag
     1021 ttatgcacgg tgtgttgttt tgaacaaaaa aaaaatcaag attagagtca cttctccaac
     1081 ttgattcatg gtggactgtt tgatttaata tacacccact ggcatgaatt ttatttgatt
     1141 caacgtctag attatttgtc cccacaatac ctatgtgagg gaaagtaaag gtgattattt
     1201 gttttgcata agatggatct gttaatattt cttggtaccc tgtctgggag gtattaaagc
     1261 atagttctcc aatattgcta gttttagcgc ccattccctg accccaataa accttgccat
     1321 tttctaaaat aagagcagct gaaggaggtt taagcgaggg atgatcatta gtgtctttca
     1381 tcatatcaga ttgaggcaaa cgagcgttta ctaggcattt taaaatgtat agtcaaggat
     1441 tagttgtatt tcatgataag agttataata ataatttttc tttaaaaagc gattctgcag
     1501 aatggataat aaaaatgagt ttacgaataa ttattgagga caaatacaaa aatgccatta
     1561 aggcaaaaaa tactattgag attaatactt ttcgtttaat aaaaagtgcg attaaggata
     1621 gggatatatc caatcgttct ggagacaata ataaaggtat tgtagattta gaaattttgt
     1681 ctttattaca aaacctcatt aagcaacgta aagattcaat tgattccttt aaagttgcct
     1741 ctaggaacga tttaattgct gtagagcaag gtgaaattga tgttattagg caatttttgc
     1801 cacaacaact caatgaagaa gatacggaaa aaataattga aaaaataatt tctgaaaata
     1861 atttttacac tctaaaagat atgggtggtt taatgaagaa tcttaaaagt agtcatgcag
     1921 gaagcattga tatggcatta gcaggaaaga tagctaaatc taaactttca taggaatgtc
     1981 ttttttaaaa actgatcttg ataaaataaa atctaaaatt tcaattagca gtgaattaga
     2041 aaaaaaagca aaattagttc aaaagggaag agattactgg tgttgctgcc cttttcatga
     2101 ggagaaaaca ccatcatgta aaattaatga tgatcaagga tctttttatt gctttggatg
     2161 cggtgctaag ggagacattt ttactcttta tacagatctt tataactata attttgttga
     2221 tgcagttaag gagcttagcc aacgtgcagg agttaatatt aaatttcagg atgcgcaaca
     2281 aaccaaacaa gaaaaccaag ttgaagctat tttaaaacta acaagtgagt ggtatcaaaa
     2341 taatcttgaa gtttcagagg ctcaaaaatg taaattatat ttggaaggtc gaaatttatc
     2401 cgatgaaact attagtcaat ttaaattagg ctattcttat aatgcctcat ccacactcta
     2461 taaattttta aaaagtaaat cttttaatga tgaggattta ctcaaaagta atgttgttaa
     2521 gttagataag aacaacaaaa ttcgagatta tttttataag cggctaattt ttcctatctt
     2581 gaacttacaa ggacatattg ttggatttgg gggaagggct ttagatgact ccaatccgaa
     2641 atatatcaat tctcctgaga gtaacatatt tcaaaaaagg catcttttat ataatttgaa
     2701 tttagctaaa aataccgcac gaaaaaaaaa caacattcta atttgtgagg gatatatgga
     2761 tgtcatttca ctttatcaaa atggtttaca gagtgtcgta gcacctttag gtacagcttt
     2821 aacagaggat caactccaac tttcttggaa atattctact aagcctacga ttatgtttga
     2881 tggagacaat gcgggtataa gggcttctta taaatctgca gttatggccc tgcctttaat
     2941 ttcagcaaaa aaatttcttc agtttgtttt gttaccagag gggtttgatc ctgacagttt
     3001 tataaataat gtttcatttg ataaatttgt tgaattatta aaaaaacctc aatctcttgc
     3061 ccattttatt ttttctcaat caagtaaatc tattaaattg aatagtgcag atgaaaaaat
     3121 atcatacgat aaatatctag atgatttggt gcaaacaatt aaagataaaa aaactcaata
     3181 tttttataaa aatgaactta agtcactttt ttttgataaa atacggcaat cgcggtcctc
     3241 atttaaggga aaaaacatta ccgtaaaaaa aattaatact tccttatata atcagcagat
     3301 attatctttt atagctagtg caattaatca tgttccaatt cgtcaaaaga ttttagaaga
     3361 tttacaggca acagatttat tgaacaatgc acaccgaaat ctgattagag atataaaatc
     3421 tgatgataat ttagatttac aa
//
LOCUS       DTVV01000286            1649 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1837999, whole genome shotgun sequence.
ACCESSION   DTVV01000286 DTVV01000000
VERSION     DTVV01000286.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1837999"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="EYO86_03410"
     CDS             <1..216
                     /locus_tag="EYO86_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase"
                     /protein_id="HIC42112.1"
                     /translation="IEATKIPPHWHAWLHKSIDKPPLNYTHKYSWQKNHEQNKTGTED
                     AYYPDSYPLSKSYNEDTIKSDYESWSP"
     gene            104..583
                     /locus_tag="EYO86_03415"
     CDS             104..583
                     /locus_tag="EYO86_03415"
                     /inference="COORDINATES: protein motif:HMM:PF09923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HIC42113.1"
                     /translation="MNKIKQELKMHITQILTLYQNLIMKTQLKVTMSLGVLRFLLLLL
                     LLFFSFSIKSEPIAGQTVVFQILDKITTKVKTFEVAVNDNVHFESLIIEIYACSTNPP
                     EEIPEDFVLLKIFDNINLENSKLIYQGWMISSSPAATPLEHPIYDLWLKDCKIDTDF"
     gene            complement(538..1191)
                     /locus_tag="EYO86_03420"
     CDS             complement(538..1191)
                     /locus_tag="EYO86_03420"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:PRK00301.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIC42114.1"
                     /translation="MRTILDTKELIQLYKKGYFPMAKSANSVEIHFFKPQKRFIIPIF
                     SFHLPRKLFSEYKKNKFTFCINTQFETVIQNCSIPRKINNETWINETIKETYLQLNLE
                     GQAHSIECFYKNKLVAGLYGVHIGSCFFGESMFNNMTNTSKLTLLYLIALLANNKFTL
                     LDSQFYNPHLLQFGAYEINDDEYQQYLHKGINKQISFPQELNFQKSVSILQSLSQRS"
     gene            complement(1188..>1649)
                     /locus_tag="EYO86_03425"
     CDS             complement(1188..>1649)
                     /locus_tag="EYO86_03425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704357.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIC42115.1"
                     /translation="RLQVEHPVTEMITHTDLVKQQILVASGQKLNFSQDDVKIQGHSI
                     ECRINAEHPETFIPSPGTVTQYHQPGGLGVRVDSAIYQEYSIPPHYDSMIAKLITYGD
                     TREVSIQKMKGALEEYVIIGIDTNIQLHQKIIKSDEFISGKYDINYMSRFD"
BASE COUNT          552 a          239 c          244 g          614 t
ORIGIN      
        1 attgaagcta caaaaatacc accgcattgg cacgcctggt tacacaaatc tattgataaa
       61 cctccattaa attatactca taaatattca tggcaaaaaa atcatgaaca aaataaaaca
      121 ggaactgaag atgcatatta cccagattct taccctctat caaaatctta taatgaagac
      181 acaattaaaa gtgactatga gtcttggagt ccttagattt cttttattac tcttattatt
      241 atttttttca ttttcaataa agtcagagcc aattgcagga caaactgttg tatttcaaat
      301 acttgataaa attacaacaa aagttaagac ttttgaagtt gctgtaaatg ataatgttca
      361 ttttgaatct ttaattatag aaatttatgc atgtagcaca aatcctccag aagagattcc
      421 agaagatttt gttttgttaa aaatttttga taatattaat ttagaaaatt caaagcttat
      481 ttatcaagga tggatgattt cctctagccc tgcagcaact cctttggaac atcctattta
      541 tgatctttgg cttaaagact gcaaaattga tactgatttt tgaaaattta attcttgagg
      601 gaaagagatt tgcttgttta ttcctttatg taaatattgc tgatattcat cgtcatttat
      661 ttcgtaagcg ccaaattgca aaagatgggg attgtaaaat tgtgaatcta gtaaagtaaa
      721 tttattatta gcaagcaaag ctataaggta gagtaatgtt aatttacttg tattggtcat
      781 attattgaac atgctttctc caaaaaaaca acttcctata tgtacaccgt ataatccagc
      841 aactagttta tttttataaa aacactcaat agagtgtgct tgaccttcaa gatttaattg
      901 caaataagtt tctttgattg tttcgtttat ccaagtttca ttattaattt ttcttgggat
      961 gctacaattt tgtataacag tttcaaattg agtattaata caaaatgtga atttattttt
     1021 tttgtattca gaaaataatt ttcgaggtaa atgaaatgaa aatataggaa taataaatct
     1081 tttttgaggt ttaaagaaat gtatttcaac actattggca ctttttgcca taggaaaata
     1141 gcctttttta tataattgaa tgagttcctt tgtatctaat attgttctca atcaaatctc
     1201 gacatataat ttatatcgta tttaccagaa ataaattcat ctgatttaat aattttttga
     1261 tgtagttgaa tatttgtatc aataccaatt ataacatatt cttctaaagc tcctttcatt
     1321 ttttggatag aaacttctct tgtatctcca tacgtaataa gtttcgctat catactatca
     1381 taatggggag ggattgaata ttcttgataa atcgcagaat ctacgcgaac acctaaacct
     1441 cctggttgat gatattgcgt tactgttcca ggggatggaa tgaatgtttc tggatgttcg
     1501 gcgtttattc gacattctat agagtgacct tgtatcttta catcatcttg agaaaaattt
     1561 aacttttggc ctgacgcgac taaaatttgt tgttttacta aatctgtgtg agttatcatt
     1621 tcggtaacag gatgctcaac ttgtaaacg
//
LOCUS       DTVV01000287            1559 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1839739, whole genome shotgun sequence.
ACCESSION   DTVV01000287 DTVV01000000
VERSION     DTVV01000287.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1839739"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="EYO86_03430"
     CDS             complement(<1..341)
                     /locus_tag="EYO86_03430"
                     /inference="COORDINATES: protein motif:HMM:PF00171.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC42116.1"
                     /translation="MGCYKFSMKKYQSYINGKWIDSLSKETIKVDDPATAKGIGEVAC
                     AKKEDVDVAVEAARKAFNSRILVDMHPMDRAKLMHKIAEELRNVSKEAGKLLCYENGK
                     PLAGAEYEFVDA"
     gene            complement(317..1513)
                     /locus_tag="EYO86_03435"
     CDS             complement(317..1513)
                     /locus_tag="EYO86_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIC42117.1"
                     /translation="MNNKLNGQTNIYDIIKKEKLDVVNKPISKAHGLPNECYNSVEYT
                     KIERKKLFEDKWVVIGVASSIPNPGNAKPFDLLGIPLIILRNNKNQIRVYHNVCSHRG
                     YKILKKSCRIKNVLRCPYHSWSYNLDGALVSTPHIGGMNKHEDENFDKSSSGLKEIRS
                     FVWLDMIIVNVSNNESSFEEYIKPLSDRWSKFWTKNDQKLIKHSKDLGYFKLKAKCNW
                     KFAIENYCESYHLPWVHPGLNAYSKISDHYHIQGLPNRFAGQGTDVYNPRLKGKNKFP
                     CFPNWPKNKINIAEYVALFPNVMLGIHKDHYYAYWLEPVNHELTIEHMEIYYVGNEAA
                     NSKKFKALRKQNLKLWHGVQSEDVSIIEGMQEGRNSPVYNGGNFSPTMDNPTHHFHKW
                     VATNLV"
BASE COUNT          481 a          275 c          218 g          585 t
ORIGIN      
        1 gcatctacaa attcatattc agcacctgca agaggtttac cattttcata acataataat
       61 ttcccagctt cttttgaaac gttacgaagt tcttctgcaa ttttatgcat taattttgct
      121 cgatccattg gatgcatatc aactaaaatt ctagaattga atgctttacg agctgcttca
      181 acagcaacat caacatcttc ttttttagca caagcaactt cacctatacc ttttgctgtt
      241 gctgggtcat caactttaat ggtttcttta gaaagactat ctatccattt accatttatg
      301 taactttgat attttttcat actaaatttg tagcaaccca tttatgaaaa tggtgagttg
      361 gattatccat agtaggtgaa aaattaccac cattgtagac aggtgagttt ctgccttcct
      421 gcattccttc aataatactt acatcttcag attgtacacc atgccacagc tttaaatttt
      481 gtttcctaag agctttaaac tttttggaat ttgcagcttc attacccaca taataaattt
      541 ccatatgttc tatagttaat tcgtgattta ctggttctag ccagtaagca taataatgat
      601 ctttgtgtat acccagcatt acattgggaa aaagagcaac atattcagca atatttattt
      661 tgttttttgg ccagttagga aaacacggaa atttattttt tcctttaagt cgtgggttat
      721 aaacatcagt tccttggccg gcaaatctat tgggtaagcc ttgaatatga taatgatcac
      781 tgatttttga ataagcattt aatccaggat gaacccaagg tagatgatag ctttcacaat
      841 aattttctat tgcaaatttc cagttacatt ttgcttttaa tttaaaatat cctaaatctt
      901 ttgaatgttt aattaatttt tggtcatttt ttgtccaaaa tttagaccaa cgatcgctta
      961 aaggtttaat atactcttca aaagaagatt cgttattaga aacatttaca ataatcatat
     1021 ccaaccacac gaaagaacga atttctttaa gtccactaga agatttgtca aaattttcat
     1081 cttcatgttt attcattcca ccaatatgag gtgttgagac taacgcaccg tctaaattgt
     1141 aggaccacga atgataggga catcttaata cattttttat acgacatgat ttttttaata
     1201 ttttgtaacc tctatgacta caaacgttgt gataaactct aatttggttt ttattatttc
     1261 gtaatataat taaaggaatt cctaataaat caaatggttt tgcattaccc ggattaggaa
     1321 ttgagcttgc gacacctata acaacccatt tatcctcaaa gagctttttt ctttctattt
     1381 ttgtatattc aacactattg tagcattcgt taggtaaacc atgagcttta ctgatgggtt
     1441 tatttacaac atccaacttt tcttttttta taatgtcgta aatatttgtc tgtccgttaa
     1501 gcttattgtt catggaaaaa tcctagcatt ttcatctaaa taggcaatag atttcgaac
//
LOCUS       DTVV01000288            2729 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1844673, whole genome shotgun sequence.
ACCESSION   DTVV01000288 DTVV01000000
VERSION     DTVV01000288.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1844673"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="EYO86_03440"
     CDS             <1..747
                     /locus_tag="EYO86_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIC42118.1"
                     /translation="KTLVNNGYNEVVLTGVDITDYGRDLPGQPNLFQLVKRILNLIPD
                     LKQLRLSSIDCAEITDDFWPLLQEKRLMPHFHLSLQAGNDLILKRMKRRHTCQQVFDF
                     CQKARSIRNDIVFGADIIAGFPTETEDMFQDSLNMILDCNLTHLHIFPYSVREDTPAA
                     RMPQVPKNIIKQRAKLLREAGERQMNKYLSTQIGISAIMLVEQTKENISFGKSQHFTK
                     IEINDVIKEGKIVHCMITNINKGVLNAHII"
     gene            752..1666
                     /gene="ftsY"
                     /locus_tag="EYO86_03445"
     CDS             752..1666
                     /gene="ftsY"
                     /locus_tag="EYO86_03445"
                     /inference="COORDINATES: protein motif:HMM:PRK10416.2-5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIC42119.1"
                     /translation="MNIFKHFQSGLKKTSNFLSTNIIGALKSKKIDQETLDEIETILL
                     SSDIGLEVTNHLIQKIQSTKIFKSENANSVLKLLANELELILQPREKPLLTSRDYQPT
                     ILIFIGVNGSGKTTTIGKLINIIPNDKKILVGACDTFRAAAVDQLKKWTKNQKVDFHQ
                     GSLEQDPASVAYNVCQKAQKEQYDYVIIDTAGRLSNNTNLLNQLVKIKSVISKKINAD
                     NIKTILVLDGTNGSNMVNQVETFGQSMNVSGLIITKIDGTAKGGALISVAKKFEFPIH
                     FVGLGEKAEDLHEFNAKKFSHSMLGIEE"
     gene            1670..2209
                     /locus_tag="EYO86_03450"
     CDS             1670..2209
                     /locus_tag="EYO86_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HIC42120.1"
                     /translation="MNSILKILVDIGPLAVFFIFYTRGDLLSAILPFMIATVIAVLFS
                     YIVAKKIPIMPTVGAAIILVFGGLTIYFDNDVFFKMKPTIINILFGAILYGGMLINKP
                     LLKYLLGNALKLQKEGWIILTKRWIGFFIALAILNEIIWRTQSTDLWVNFKVFGILPI
                     TFIFTMTQFSLIKKYQIED"
     gene            complement(2210..>2729)
                     /gene="pgk"
                     /locus_tag="EYO86_03455"
     CDS             complement(2210..>2729)
                     /gene="pgk"
                     /locus_tag="EYO86_03455"
                     /inference="COORDINATES: protein motif:HMM:PF00162.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIC42121.1"
                     /translation="QNIDIGNSLCEKDLSTTALSIMEKADNLNCKIVLPLDVVCADNI
                     NDQSSIRRCDIKNILPNQMVLDVGNKTSKIIAEIILRARMVLWNGPLGAFEYKPFEQS
                     SVEVANVIKNNAKILNITTLAGGGDTISAIKMAKAEEGFSYISKAGGAFLEWLEGKES
                     PGVTALKENTYN"
BASE COUNT          963 a          456 c          427 g          883 t
ORIGIN      
        1 aaaaccttag tgaacaatgg atacaatgaa gtggttctca ctggagttga tattactgac
       61 tatggaagag atcttccagg tcaacctaat cttttccaac tagtcaaacg tattttaaat
      121 cttattcccg atctcaaaca actgcgacta tcctcgattg attgtgcaga aatcaccgat
      181 gatttctggc ccttgcttca agaaaaaagg ttgatgccac attttcactt aagcttacaa
      241 gcgggtaatg atttaatcct caagagaatg aaaaggcggc atacttgtca acaagttttt
      301 gatttttgtc aaaaggcgcg ctctataaga aatgacatag tatttggagc tgacattatt
      361 gctggttttc ctacagaaac agaagatatg tttcaagatt ccttaaatat gattctggat
      421 tgtaatctta cacatctgca tatctttcct tattcagtaa gagaggacac tcctgcagca
      481 cgtatgccac aagttccaaa aaatataata aaacaaagag caaaactttt acgagaagca
      541 ggtgagagac aaatgaataa gtatcttagc actcagatag gaatatcagc catcatgctt
      601 gtagaacaaa caaaagaaaa tatttcattt ggtaaatctc aacattttac taaaattgaa
      661 attaatgatg taattaagga agggaaaatt gttcactgta tgataactaa tataaataag
      721 ggtgtcttaa acgctcatat tatttgagat aatgaacata tttaaacatt ttcaatcagg
      781 tcttaaaaaa acatcaaatt ttctttccac aaacattata ggcgcactta aatcaaaaaa
      841 aattgatcaa gaaacactag atgagataga aacaattcta ttatcatctg atattggtct
      901 agaagttaca aatcacttaa ttcaaaaaat acaatcgaca aaaattttta agtcagaaaa
      961 cgctaactca gttttaaaac ttctagcaaa tgaattagaa ttaattcttc aaccaagaga
     1021 aaagcccctc cttacatcta gagactatca accaacaatt ttgattttta tcggtgttaa
     1081 cggcagtgga aaaacaacaa ctataggtaa attaattaac atcattccta atgataaaaa
     1141 aattttagtt ggtgcttgcg atacttttcg agcagccgcc gtggatcaat taaaaaaatg
     1201 gaccaaaaat caaaaagtag attttcatca aggttcactt gaacaagatc ctgctagcgt
     1261 tgcctataat gtctgccaaa aagcacaaaa agagcaatat gattatgtta tcattgatac
     1321 tgctggacgt ttatcaaata acactaattt attaaatcaa ttggtaaaaa ttaaatctgt
     1381 tattagcaaa aaaattaatg ctgataatat taaaacaatt cttgtgttgg atggaaccaa
     1441 cggttccaat atggttaatc aagtagaaac atttggccaa tcaatgaatg ttagtggatt
     1501 aattattaca aaaatagatg gtacagcaaa aggaggggcg ctcatttcag ttgcaaaaaa
     1561 atttgaattc cctatacact tcgtaggtct tggcgaaaaa gcagaagatt tacatgaatt
     1621 taatgcaaaa aaattttctc actcaatgct gggaatagag gaataataaa tgaatagtat
     1681 tttaaaaata ttggtggata tcggtccgct agccgtattt tttatttttt atactcgtgg
     1741 agacttgctg tctgccatcc tacctttcat gattgcgacc gttattgcag tgttattctc
     1801 ttacattgtt gcaaaaaaaa tacctattat gccaactgta ggcgctgcta ttattctcgt
     1861 attcggtggg ttaactattt attttgataa tgacgttttt ttcaaaatga aaccaacaat
     1921 cattaatatt ttatttggag caatccttta tggaggaatg ctaattaata agcctctttt
     1981 aaaatatctt ttagggaacg cattaaagtt acaaaaagag ggatggatta tattgaccaa
     2041 acggtggatt ggttttttta ttgctctagc aatactaaat gaaattattt ggagaacaca
     2101 gtcaaccgat ctgtgggtta atttcaaagt atttggcatt cttccaataa ctttcatttt
     2161 tactatgact caattttctt tgataaaaaa atatcaaata gaggactaac taattatatg
     2221 tattttcttt gagtgccgtc actcctggtg attcttttcc ttcaagccac tctaaaaatg
     2281 ctcctccagc tttagatata taagaaaaac cttcctcagc ttttgccatt ttaattgcag
     2341 aaattgtatc tcccccacct gcaagagttg taatatttaa tattttggca ttatttttaa
     2401 taacattcgc tacttcaaca gaagattgtt caaatggctt atattcaaaa gcccctaagg
     2461 ggccgttcca caaaaccatg cgagctcgca aaataatttc cgcaattatt tttgaagttt
     2521 tgttaccaac atccaagacc atttggttgg gcaaaatgtt ttttatgtca caacgacgaa
     2581 tacttgattg atcattaata ttatctgcac aaacaacatc taatggtaat acaattttac
     2641 aattcaaatt atcagctttt tccattatgg atagcgccgt ggttgataaa tctttttcac
     2701 ataatgaatt tccaatatca atattctgt
//
LOCUS       DTVV01000289            1647 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1846920, whole genome shotgun sequence.
ACCESSION   DTVV01000289 DTVV01000000
VERSION     DTVV01000289.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1846920"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="EYO86_03460"
     CDS             complement(<1..773)
                     /locus_tag="EYO86_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIC42122.1"
                     /translation="MKIGYFCNATNWKNQKSYSEILGEIREIATYCDENDWDSIWFTE
                     HHFSHEGLEICPNPLMISADIAARTKNIRIGQAATIVTFWNPIRVAEDIALLDNLSNG
                     RVEAGIGRGIYGREALNMNPDADTKDQAKNFRLFEETITILKKAWSQQYFNHEGEFYT
                     YPAPNFVWRHDMSPPSSEFMNIETNILEKISIVPRPIQQPHPPLHQVVDSPSSIEWAA
                     KNGINTIMWIPTVQSLKKRFEIYKNARSETEKRNVEMGEG"
     gene            946..1605
                     /locus_tag="EYO86_03465"
     CDS             946..1605
                     /locus_tag="EYO86_03465"
                     /inference="COORDINATES: protein motif:HMM:PF13419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HIC42123.1"
                     /translation="MIKNIIFDFDGVLVDSEMLVAQSFCLYLCERNIQFTENDFSQFA
                     GKKTVNVISELSAKFNIDNEQGFFNDIMQITNEIYVNELTPVPGALEFLESTSYNCFI
                     GSNSVKERVVSGLQKVKLDKFFAVNKVFSFDMIGKPKPEPDIYLAVINANNLNKEETI
                     ILEDSVVGVKAGVASGVKVVGVTAGGHWYKNRPTQELLDAGAFVLIDNYKKLHIEINK
                     L"
BASE COUNT          525 a          277 c          280 g          565 t
ORIGIN      
        1 ccttctccca tttcaacatt tcttttttca gtttctgatc ttgcattttt atatatttca
       61 aagcgttttt ttaaggattg tacagtcggt atccacataa tcgtgttaat tccattctta
      121 gcggcccatt ctattgagct aggactatcc actacttggt gaagaggagg atgaggttgt
      181 tgaatgggtc ttggcacaat acttattttt tccaaaatat tggtttctat attcataaat
      241 tcactactag gcgggctcat gtcatgtctc catacaaagt tcggagcagg gtatgtataa
      301 aactcacctt catggttaaa atattgttgt gaccatgctt ttttcaaaat ggttattgtt
      361 tcttcaaata atcggaaatt ttttgcttga tctttagtat cagcatctgg attcatattt
      421 aatgcttctc gcccatatat gccacgccct atacccgctt ctactcgtcc atttgataaa
      481 ttgtctaaca aagcaatgtc ttcagctaca cgaataggat tccaaaaagt cactatggtt
      541 gcggcttgtc caattcgaat attcttagtc cgagccgcta tatcagcact tatcattaac
      601 ggatttgggc aaatttccaa accttcatga ctgaaatgat gttctgtgaa ccaaattgaa
      661 tcccagtcat tttcatcaca atatgtagca atctccctta tttcacctaa aatttcactg
      721 taagattttt gatttttcca atttgttgca ttgcagaaat agcctatttt cattatacct
      781 ccttaagttt gaaggcgatc tatcccactt tcgctcagaa acgacgagtt atgtatcgct
      841 caaattataa tattcaataa tatcaaaaaa acaatcaata gatctcataa ctctcattaa
      901 aagctgtgat attctagaaa aatcattaga aacaactttt tttaaatgat aaaaaatatt
      961 atttttgatt ttgatggtgt gttggttgac agcgaaatgt tagttgctca gtcgttttgt
     1021 ctatatcttt gtgaaagaaa tattcagttt acagaaaatg acttttcaca atttgccggt
     1081 aaaaaaacag ttaatgtaat ctcagaatta tcagcaaaat tcaatattga taacgagcaa
     1141 gggtttttta atgatattat gcaaattacc aatgaaattt atgtaaatga attaacccct
     1201 gttcctggag ctctagagtt tcttgaatca acttcatata attgttttat aggctctaac
     1261 agcgtgaaag aaagggttgt ttcaggatta cagaaagtaa aacttgataa gttttttgca
     1321 gtgaataaag ttttttcttt tgatatgatt ggcaaaccca aacctgagcc ggatatatat
     1381 ttagcggtaa taaatgctaa taacttaaat aaagaagaaa caataatact tgaagacagt
     1441 gtggttggag taaaggcagg agtggcttct ggggtaaagg ttgtaggcgt aacagcaggt
     1501 ggtcattggt ataagaaccg tccaacacaa gaactattgg atgctggggc gtttgtttta
     1561 atagataatt acaaaaaatt acatatagag ataaataaat tatgactgac agaccaaaaa
     1621 taaaaggcaa tccacttctt gtgacag
//
LOCUS       DTVV01000290            1289 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1854597, whole genome shotgun sequence.
ACCESSION   DTVV01000290 DTVV01000000
VERSION     DTVV01000290.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1854597"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(85..465)
                     /locus_tag="EYO86_03470"
     CDS             complement(85..465)
                     /locus_tag="EYO86_03470"
                     /inference="COORDINATES: protein motif:HMM:PF05523.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42124.1"
                     /translation="MSSVKKYQLNPILSDERGLFFEVLNKINISHVIVTTFKKNAIRG
                     NQYRKNMDQYFFLTSGKLKLITKKPNDSDDKKEMDMIQGTMVFIPKGIAFVTKAIEDS
                     ILLELSPDHFDPENPDINKYNIDI"
     gene            569..>1289
                     /locus_tag="EYO86_03475"
     CDS             569..>1289
                     /locus_tag="EYO86_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIC42125.1"
                     /translation="MVSNESFWNESNVLVTGGASFIGSHIVDKLVSYDANVTVLDDLS
                     SGTLENLSKSLDKVKFVTHNLEYNAKDVLKTLFKDQDYVFHMAAIHGGRGYIDTHPAD
                     VCSNLALDHHVFQACSDSNVQKVIFSSTACVYPTKLQDKVGSNYKLRETDSDITNLDG
                     KFSADMEYGWAKLMSEIQMRAFHKQYGLNGCSLRFVTAYGPRENETHAIIALIYKAVE
                     KMDPYQIWGDGNQERDFTYVED"
BASE COUNT          459 a          175 c          221 g          434 t
ORIGIN      
        1 acaatcaccg ctgctacaaa cattaaacat aggattcgta tgactagatt atactttcat
       61 gctgaaaaaa ataatttgaa taaatcaaat atctatattg tatttattaa tatctggatt
      121 ttcaggatca aaatgatcag gtgataattc taataagatc gagtcttcaa ttgccttggt
      181 aacaaatgca ataccttttg gaatgaaaac catagtaccc tgaatcatat ccatttcttt
      241 tttatcatca gaatcattcg gtttctttgt aattaattta agtttgccag atgtaagaaa
      301 aaaatactga tccatgtttt tacgatattg atttccacgt atcgcatttt ttttaaacgt
      361 tgtaacaata acatgtgata tgtttatctt attcagtact tcaaaaaaca gacctctttc
      421 atcagaaagt atgggattta attgatattt tttgactgaa gacattgaac agtttttgaa
      481 tgatttttca tattaaatag ttttaagaag tattttaaaa aaagaaatat gtagaaacca
      541 taatatagaa attataaaaa aatgtctcat cgtgtctaat gaatcctttt ggaatgaatc
      601 aaatgtgttg gtaactggcg gagcaagttt tattggcagt catatagttg acaagctagt
      661 ttcatacgat gctaatgtaa ctgtacttga tgatttgtca agtgggacat tagaaaattt
      721 atctaaatct ctagataaag taaaatttgt aacccataat ttggagtata acgcaaaaga
      781 tgttctaaaa actctgttca aagatcaaga ttatgtattt catatggcag caatacatgg
      841 cggaagggga tatattgata ctcatcctgc tgatgtttgc tcaaatttag ctcttgatca
      901 tcatgtattt caagcttgta gcgatagtaa tgttcaaaaa gttatttttt caagtactgc
      961 ttgtgtatat ccaaccaagc ttcaagataa agttggttct aattacaaac taagggaaac
     1021 tgattccgat ataactaatc ttgatggaaa gtttagtgcc gatatggaat atggatgggc
     1081 aaaactaatg agtgaaatac agatgagggc atttcataaa caatatgggt taaatggttg
     1141 ctcattacga tttgtaacag cttatggacc tagagaaaat gaaacgcatg caataattgc
     1201 cctgatctat aaagctgttg aaaaaatgga tccctatcaa atatggggag atggaaatca
     1261 agaaagggac tttacatatg ttgaagata
//
LOCUS       DTVV01000291            2825 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1856005, whole genome shotgun sequence.
ACCESSION   DTVV01000291 DTVV01000000
VERSION     DTVV01000291.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1856005"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(128..412)
                     /locus_tag="EYO86_03480"
     CDS             complement(128..412)
                     /locus_tag="EYO86_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase F0F1"
                     /protein_id="HIC42126.1"
                     /translation="MDNNNDKISDLKKKINSIEKSHQSESMPKKESGVSFGFKISTEL
                     VAALVVGVGIGLLVDNYFNTKPFGLIIFFILGAFAGFFNVYRVMRRIEKQ"
     gene            complement(405..>2825)
                     /locus_tag="EYO86_03485"
     CDS             complement(405..>2825)
                     /locus_tag="EYO86_03485"
                     /inference="COORDINATES: protein motif:HMM:PF02463.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42127.1"
                     /translation="DRPAWDIAEVTLDLDNSERKAPSIFNQNDKIEVTRRIWRGEGSE
                     YRVNGKEVRLKDVQLLFADASTGARSTSMVSQGRVGAIIAAKPEQRRSLLEEAAGITG
                     LHSRRHEAELRLNATENNLERVKDVLETQDEQLEILKKQSKQAERYKNIQKDITRARA
                     TVFYQKWQTEKNKLEETTQKIYKQNSLVVDQTQVVAKINIGYENVQQSLPNLRLEENK
                     IAAELQRNSINLDNQEKEIERANTAIEEIQIRIEQIKNDSNREKFLFDDAQENLSRVR
                     DEKSLLEKQQGDLFVEENNDDLNHVTSPTKNNNPIIDYLNFEDGYEKALAAVFSDELM
                     ASISDKHSSFWRTLQSNNQSSFPEGIIPFSTIVKAPDNLKKRLEYIGFIQNKENINDL
                     QNNLSDGQILVSELGEIWRWDGFTSKGKQSTLTKAVLEQLKNRRLKQLSAEEKQWQDI
                     MITAQKRIDELHERKKTLNNDLLEMESMPKNISSEKTRLQNLITENKITYDKVAGKLR
                     DTEQQANEINKKLKLEEVRLNELREEKIRIEGVIGTLNETIHQLTDQVRERLSIKMEA
                     LFEVAKINPNKTLPSLEDLERKLERLIGEQERLGGVNLLAEQEGAELEKKVNIIKKDQ
                     SDLLAAISKLREAIDTLNTEGRQRLLASYGVVNEHFQRLFVQLFGGGKAYLKFTDESD
                     PLEAGLEIYASPPGKKLQNLTLMSGGEQALTALSLLFAVFMSNPAPICVLDEVDAPLD
                     DTNVDRFCSLVEEISQNSLTKFLVITHHRMTMARVNRLFGVTMPEKGVSQLVSVDLEK
                     AVQIRENG"
BASE COUNT          769 a          545 c          453 g         1058 t
ORIGIN      
        1 gaattggtaa aggcaagatc cacactaccc acctcaaagt gcacgaaggg cgtaatttca
       61 aactgttcaa gcggactgtg tttttctcct gaagcggaca ttttatatct gtgtgatatt
      121 tgaattctta ttgtttttca atgcgacgca ttacacgata aacattaaaa aatccagcaa
      181 atgcaccaag aatgaaaaaa ataatcagtc caaaaggttt ggtattaaag taattatcca
      241 caagaagccc gatacctaca cctacgacta aagcagctac taactctgta cttattttaa
      301 atccaaagct aacaccactt tcttttttag gcatcgattc ggattgatga cttttttcga
      361 tactgttaat ttttttcttt aagtcagaaa tcttgtcatt attattatcc attttctctt
      421 atttgaacag ctttctctaa atctacggat acaagttgtg aaacaccctt ttcaggcatc
      481 gtcactccaa ataacctgtt aactctagcc atggtcattc gatgatgtgt aataacaaga
      541 aatttcgtta gagaattttg agaaatttct tctaccaatg aacaaaaacg atcaacattt
      601 gtatcatcaa gaggggcatc aacctcatca agaacacaaa taggggctgg attggacata
      661 aacacagcaa acagtaaaga tagtgcggtt aaggcttgtt ccccaccaga cattagtgtc
      721 aggttttgca atttttttcc aggcggactt gcataaattt ctaatcccgc ttctaaagga
      781 tcgctttcat ctgtgaattt taaataggct tttcctcctc cgaagagttg aacaaatagt
      841 ctttgaaaat gttcatttac tacaccgtac gaagcaagta agcgttgacg accttcagta
      901 tttaatgtgt caatggcttc acgtaatttt gaaattgccg ctagtagatc agattgatcc
      961 tttttaatta tatttacctt tttttccaat tctgccccct cttgctctgc taacaaatta
     1021 acacccccta aacgttcttg ttcaccaata agacgttcta gttttctctc tagatcttct
     1081 aatgagggaa gggttttgtt tggattaatt tttgctactt caaataaagc ctccattttt
     1141 atgcttaatc tttcccgtac ctgatccgtt agttggtgaa tagtttcatt taatgttcca
     1201 ataacgcctt caatgcgaat tttttcttct cgtaattcat tgagccttac ttcctctaat
     1261 tttaattttt tgtttatttc attagcttgt tgttccgtgt cacgaagttt tccagcaact
     1321 ttatcataag tgattttatt ttcagtaatt aaattttgta aacgtgtttt ttctgaagag
     1381 atatttttgg gcatggattc catttctaat aaatcattat tcaatgtttt ttttcgttcg
     1441 tgcaattcat caattctttt ttgtgctgtg atcatgatgt cttgccattg tttttcttca
     1501 gcagataatt gttttaggcg cctgtttttt aattgttcta acacggcttt ggttaatgta
     1561 ctttgtttac ctttagaggt aaagccatcc caacgccaaa tttcccctag ttcactcacc
     1621 aaaatctgac catcagaaag gttattttga agatcgttaa tattttcttt attttgaata
     1681 aaacctatat attccaagcg ttttttgagg ttatcgggag ctttaacgat agttgaaaaa
     1741 gggataatcc cctctggaaa acttgactga ttgttgcttt gtagtgttcg ccaaaaggac
     1801 gaatgtttat cactaataga ggccattaat tcatcagaaa aaacagcagc aagggctttt
     1861 tcatatccat cttcaaagtt taagtaatca attataggat tgttattttt tgttggagat
     1921 gttacatgat tcaaatcatc attattttct tcaacaaata aatccccttg ttgtttttcc
     1981 aacaatgatt tttcatcacg aacacgagat aagttttctt gagcatcatc aaataaaaat
     2041 ttttctctat tgctatcgtt tttaatttgc tcaatgcgta tttgaatctc ctcaatagct
     2101 gtgttagcac gctcaatttc tttttcctgg ttatcaagat taatggaatt gcgctgtaat
     2161 tctgctgcaa ttttattttc ttctaagcgc aaattaggaa gggattgctg cacattttca
     2221 tagcctatat taattttagc aactacttgt gtttggtcca ctaccaggct attttgttta
     2281 taaatttttt gtgttgtttc ttctaattta tttttttctg tttgccattt ttgatagaat
     2341 acagttgccc ttgcacgagt aatatctttt tgaatatttt tataacgctc tgcctgtttc
     2401 gattgttttt taagaatttc caattgttca tcttgtgttt caagtacatc ttttacacgt
     2461 tctaaattat tttcagttgc atttagtcga agttcagctt catgtcgccg tgaatgcaat
     2521 cctgtaatgc cagctgcttc ttcaagtagg cttcggcgtt gttccggttt agctgcaata
     2581 attgccccta ctcttccttg gcttaccatg gaagttgatc ttgctcccgt agaggcatcc
     2641 gcaaataaaa gttgtacatc tttaaggcga acttcttttc cgtttacacg gtactcggaa
     2701 ccctctcctc tccaaatacg gcgtgttact tctattttat cattttgatt aaaaatactc
     2761 ggggccttgc gctcagagtt gtcaagatct aaggttactt ctgctatatc ccatgcaggg
     2821 cgatc
//
LOCUS       DTVV01000292            1200 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1857217, whole genome shotgun sequence.
ACCESSION   DTVV01000292 DTVV01000000
VERSION     DTVV01000292.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1857217"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(61..435)
                     /locus_tag="EYO86_03490"
                     /pseudo
     CDS             complement(61..435)
                     /locus_tag="EYO86_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(431..1048)
                     /locus_tag="EYO86_03495"
     CDS             complement(431..1048)
                     /locus_tag="EYO86_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42128.1"
                     /translation="MQTRKVTWFVSVFITMFTPSIGAVEKINISKKQAEKSEWTTTFY
                     SAKFTNGTLHELLQFESEITNNKIYILALTRKLNTFYKKFDWEVEGQISKNVGPRQKN
                     WELNIPIIIRWNILKFQRKILKSFAAGVGLSYASKPPEYEIDLHGDSSKLLLYNMVEI
                     DFFLPNYPSLFFVARIHHRSTMFQFFNQNNRVASNALGFGLKYRF"
BASE COUNT          369 a          188 c          174 g          469 t
ORIGIN      
        1 tatcgaatca agtaataatt caatttttag gataattgta tgaaaatctt gatatgttct
       61 ctaattaggt agaggtttag actttgcctg ttctaaatct ggaacatctg gataagtagg
      121 ttgtccgttc aatatgtttt gtatttcctt cctctcatct tcttcactca accagtaagg
      181 tattctgcat attttatgac cctttgactt tgccaggttt gtttttttaa tatcgttttg
      241 atggttttgt tcaatatttt tcatcctact gccaacaatc ttaaatggtt cataatagcg
      301 ctgttctcca tccctttcaa taattaggtt gtagtcgggt agatagaaat caaaataacg
      361 attgtttatt ctgtgatttc taagaacaac tgccatttca tttaaaaata ttgctagtct
      421 gccttcgtta ttagaatcga tattttaatc caaaaccaag tgcgttagat gcaactctat
      481 tattttggtt aaaaaattga aacatagttg aacggtgatg gatccttgca acaaaaaaca
      541 aagaaggata attaggtaag aaaaaatcta tttcaaccat gttgtacaat aaaagtttac
      601 ttgaatcacc atgtaaatca atttcatatt ccggaggttt gctggcgtat gataatccaa
      661 cacctgctgc aaaacttttt aatatctttc tttgaaattt taatatattc cacctaatga
      721 taattggtat atttaattcc cagtttttct gccttggtcc gacattttta gaaatctgtc
      781 cttcaacttc ccagtcaaat tttttataga aagtatttag tttacgagta agtgctaaaa
      841 tgtaaatttt attattagtt atttcgctct caaactgcaa cagttcatgt aaagtaccgt
      901 ttgtaaattt tgctgaataa aaagttgttg tccattctga tttttctgcc tgttttttac
      961 taatgtttat tttctcaact gcacctatgg aaggcgtgaa catagtaata aaaacagaga
     1021 caaaccatgt aactttccgt gtctgcattt tttaaatttt aatgtaaata tatttactat
     1081 tttattttaa tcattgtcat tatatttctt tattgataat catattttgg caggaaaaat
     1141 tttctggaaa tcagacatga cattgttttt tgagaaatcc taatgcgaaa tctctctaaa
//
LOCUS       DTVV01000293            1307 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1862817, whole genome shotgun sequence.
ACCESSION   DTVV01000293 DTVV01000000
VERSION     DTVV01000293.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1862817"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            3..872
                     /locus_tag="EYO86_03500"
     CDS             3..872
                     /locus_tag="EYO86_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC42129.1"
                     /translation="MQLIKRIPGPLLVFLGAFCLSFGGLIVKSFESANLWQILFWRQT
                     FFAIIVALYLLVSYKKNIFKSFYNSGLSGFIAGFVLSIGFSAYVFSMYNTTVANTNFI
                     ITTETIFLAVFGYFFLKEKINLITFISIIFGMSGVLLILGSSLSIQSNEQFIGNIVAF
                     LAPISFAILVIIIRKSPNVDMVPAQFIAGVFSAILAYFIASNLSISPHDLFLASLSGF
                     FQIGFGFILITIGSQTTPSAVVGVLMLTEAVFGPLWAWLFINEIPPTSVLIGGGIIIF
                     SILLQSFYNKKNH"
     gene            959..>1307
                     /locus_tag="EYO86_03505"
     CDS             959..>1307
                     /locus_tag="EYO86_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HIC42130.1"
                     /translation="MIKIFRNSFTAIFLSLILSTFSFSAFAKGKLTLYCSVEIDVCEL
                     LVQAFEKETGINVAMTRKSSGETFAQVKAEASNPKGDVWYGGTGDPHLTASQEGLTME
                     YKSIYFDELIPAAQ"
BASE COUNT          395 a          185 c          224 g          503 t
ORIGIN      
        1 ggatgcaatt aataaaaaga attccaggtc cgttactggt tttcttaggt gctttttgtt
       61 taagttttgg aggtttaatt gttaaatctt ttgaaagtgc taatctatgg caaattcttt
      121 tttggagaca gacttttttt gctatcattg tggccttata tttacttgtc agttacaaaa
      181 aaaatatttt taagtcattt tataactcgg gtctttcagg ttttatagct ggatttgttt
      241 tatccattgg cttttcagcc tatgtttttt ctatgtataa tacgactgta gcaaatacga
      301 attttataat cacgacagag acaatttttt tagcagtatt tggatatttt tttttgaaag
      361 aaaagattaa tttaataact tttatttcaa ttatttttgg tatgtcaggg gttttgttga
      421 tacttggaag ctccttgtcc attcaatcca acgaacaatt tattggaaat attgtggcat
      481 ttttagcgcc aatctctttt gcaatattgg ttataataat aagaaaatct ccaaatgttg
      541 atatggttcc ggctcaattc atagctggag tattttccgc aattcttgct tactttattg
      601 cttccaactt atcaatttct ccacatgatc tttttttagc ttcattgtct ggtttttttc
      661 aaattggatt tggatttatt ttaatcacta ttgggtcaca aaccacacct tccgctgtag
      721 ttggcgtttt aatgttaact gaagcggtgt ttgggccttt atgggcttgg ttgtttataa
      781 atgaaatacc accaacaagt gtgctgatag gtggtggaat tattattttt tcaattttgt
      841 tacagtcatt ttacaacaaa aaaaatcatt aaatttaaaa aataataagt atattaaaaa
      901 aaacttgtaa cattattgta aaaaagagat aatctttcca ataatataag gaggtacaat
      961 gattaaaatt ttcagaaata gttttactgc tatttttcta tcattaatct tatcaacttt
     1021 ttctttctct gcttttgcaa agggtaagtt aactttgtat tgcagtgtgg aaattgatgt
     1081 atgcgaacta ttagtacaag cttttgaaaa agaaactggt atcaatgttg caatgactag
     1141 aaaaagttcg ggtgaaacat ttgcacaagt aaaggctgaa gcttcgaatc ccaaagggga
     1201 cgtatggtat ggcggaacag gtgaccctca tcttacagct tcacaagaag gtttaactat
     1261 ggagtataaa tcaatatact ttgatgaact aattccggca gctcaac
//
LOCUS       DTVV01000294            1129 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1865840, whole genome shotgun sequence.
ACCESSION   DTVV01000294 DTVV01000000
VERSION     DTVV01000294.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1865840"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="EYO86_03510"
     CDS             <1..989
                     /locus_tag="EYO86_03510"
                     /inference="COORDINATES: protein motif:HMM:PF13102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIC42131.1"
                     /translation="ITFVHPITKKWASKTVRCSYKDALKIKADIEKDLAFGKIGKPNP
                     DFRKTFFSQLKSKYIKDSKRNKTIKTTKREEIVLENFTSFLEKDMELSKITTEKIEGF
                     KDFRLENGKKPATVSIELRVLKTMFNRAIDWGLISANPVKGVKLPKHDNISVRFLTKN
                     EVEKLISTIKADENIQFLRIVKAYLHTGARRNELLPPLFTWDNVDFDERKVMINGQKG
                     QSKRFLPMNQTLFELFTEIKDNDPKTPFNFNPDYITKKIAKYYKSAKIKGANTHSLRK
                     TFGSLLIQNNVADIYTVSKLLGHASVKTTEKYYVDLVDDNYKSALDGLNDII"
BASE COUNT          424 a          167 c          216 g          322 t
ORIGIN      
        1 aaattacctt tgtgcaccct attacaaaga aatgggcatc aaaaactgta cggtgttctt
       61 acaaggatgc attgaagata aaagctgata tagaaaaaga tttagcattt ggaaaaatag
      121 gaaagcccaa cccggatttt agaaagacct tcttctccca gctgaaatca aaatatatta
      181 aagattccaa acggaataaa acaataaaaa cgacaaagag ggaagagatt gtattggaaa
      241 atttcacaag ttttttagaa aaggatatgg aactaagcaa aatcaccaca gaaaaaatag
      301 aaggatttaa ggactttcga ttagaaaacg gtaaaaagcc tgctacagtc tccattgaat
      361 tgcgtgtatt aaaaacaatg tttaaccgag caattgattg gggattaatc agtgctaatc
      421 cggttaaagg ggtcaaactg cccaagcatg acaatatttc cgttaggttt ttaacaaaaa
      481 atgaagtgga aaaattgatt tcaactatta aggctgacga gaatatccaa tttcttcgta
      541 ttgtaaaagc gtatttacat acgggtgcaa ggcgtaatga gttactgcca ccgctattta
      601 cctgggataa tgtggatttt gatgaaagga aagtgatgat taacgggcag aaaggtcaat
      661 caaaacggtt tctaccaatg aatcaaacat tgtttgaatt gtttacagag atcaaagata
      721 atgatcctaa aacaccgttt aactttaatc ctgactatat tacgaagaaa attgcaaagt
      781 attataagtc tgccaagatt aaaggtgcga atacccactc actgaggaag acgtttggat
      841 ccttactgat ccagaataat gtagcggata tttacactgt atcaaagctg cttggtcacg
      901 cttcagttaa aacaactgag aagtattatg tggatttagt tgatgataac tataagtcag
      961 cattagatgg attgaatgac attatatgac attaccataa gtcagtatgt ttctataaaa
     1021 agcttctact ttaggagctt tttttattat aaaaatggtt atttaatatt gaataaatga
     1081 aaaacgaaga ctggtatacg acaggtggtt acttcaaaaa cggtctatt
//
LOCUS       DTVV01000295            1249 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1866671, whole genome shotgun sequence.
ACCESSION   DTVV01000295 DTVV01000000
VERSION     DTVV01000295.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1866671"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            3..893
                     /locus_tag="EYO86_03515"
     CDS             3..893
                     /locus_tag="EYO86_03515"
                     /inference="COORDINATES: protein motif:HMM:PF04116.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIC42132.1"
                     /translation="MNKLLKIWVPYISYPFVISFCFLLHIVLIYFDLHLTLATWIAVL
                     FGLLSIITLERFVPCRVEWIPKNKDWIVDLFYMVLVQILLVKFMTFIVSIALLNIFQS
                     NGFTIKNLWPKEWPIGAQAILMVLIADFFRYWLHRLSHEWMPLWRLHAVHHSPHKLYS
                     MNVGRFHPLEKSIQFIFDALPFIILGITENVLAIYFVFYSINGFFQHCNIKIYLGPLN
                     YVISGPELHRWHHSMIIKESNKNFGNNLIVWDLLFGTWHLPKAKKVDQLGLINRNYPL
                     GLIEQLKSPFINGLDQDKSD"
     gene            937..>1249
                     /locus_tag="EYO86_03520"
     CDS             937..>1249
                     /locus_tag="EYO86_03520"
                     /inference="COORDINATES: protein motif:HMM:PF03321.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42133.1"
                     /translation="MMWIRKKHFVPFIELTKNPKKNQWNLLKNILHQNKDTHFGSKHK
                     FTSIHSYKDFQNAIQINEYEDLRSYINQQEQTGEPYLNSQQPVMYAQTSGTTGEPKNI
                     PI"
BASE COUNT          421 a          214 c          208 g          406 t
ORIGIN      
        1 aaatgaataa attgttaaaa atatgggttc cgtatatctc ctacccattc gtcataagct
       61 tctgcttctt gcttcatatt gtactaattt attttgattt acatctcaca ttggcaacgt
      121 ggatagccgt tttatttggc cttcttagca taataacgct cgagagattc gttccatgca
      181 gagtagagtg gataccgaaa aataaggact ggattgttga cttattttac atggttttag
      241 ttcaaatatt actagtcaaa ttcatgactt ttatagtgag tatagcctta ctgaatattt
      301 tccaatcaaa tggttttacc ataaaaaacc tttggcccaa agaatggcca ataggagctc
      361 aagcaattct catggttttg atagcagatt tttttcgtta ttggctgcat agattaagtc
      421 atgaatggat gcccttatgg cgcttacatg cggtacatca ttcacctcat aaattgtatt
      481 ccatgaatgt cggccgcttt catcccttgg agaaatcaat tcaatttatt tttgatgctt
      541 tgccatttat aatactaggt atcacagaaa atgttttagc tatctatttt gtcttttact
      601 cgattaatgg cttttttcaa cattgcaaca ttaagattta tttaggacca ctaaactatg
      661 ttatcagtgg accggaattg caccgttggc atcactcgat gataataaaa gaatctaata
      721 aaaactttgg aaataacttg atagtttggg acttattgtt tgggacttgg catctaccaa
      781 aagcaaaaaa agtagatcag ttagggttaa taaatagaaa ttatcccttg gggttaattg
      841 aacaattaaa aagccccttc attaatgggt tagatcagga caaaagtgat tgatttatta
      901 aaacaatgtt ttcttaggat ttgcctccgc ataaggatga tgtggatcag aaaaaaacat
      961 tttgttccat ttattgaatt aactaagaac ccaaaaaaaa atcagtggaa tctattaaaa
     1021 aatattttac atcaaaacaa ggacacgcat tttggaagca aacataagtt tacatcaatt
     1081 cattcctata aggattttca aaatgcaatc caaattaatg aatatgaaga cttgcgttct
     1141 tacattaacc aacaagaaca aacgggagaa ccatatctga actcacagca accagttatg
     1201 tacgctcaaa caagcgggac tactggcgaa ccaaaaaata tcccgatac
//
LOCUS       DTVV01000296            1205 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1875188, whole genome shotgun sequence.
ACCESSION   DTVV01000296 DTVV01000000
VERSION     DTVV01000296.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1875188"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(22..1023)
                     /locus_tag="EYO86_03525"
     CDS             complement(22..1023)
                     /locus_tag="EYO86_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HIC42134.1"
                     /translation="MIKSFFFSKKWRLWAWGGFLFIIGSLIAQTWIDVKINQWYKGFY
                     DLLQKATESDVSEFYDGIFLFMKLAIPYVIISTITNYFTRLYAFRWREAMTFAYMPYW
                     KEVDAKVEGASQRIQEDCMNFARIVESIGLQIIRAFMLLIAFIPILWGLSSNVILPWL
                     KDISGSLVYVSLTASLGGVLISWLVGIKLPGLEYNNQVVEAAFRKELVYGEDDRAEYA
                     KPPTVLELFTGIKFNYHRLFLHYGYFDLWLIMYNQSMIIVPYIIMGPGLFTGAMTLGI
                     LIQTSSAFREVQGSFSLFMQNWTRITELRSIFRRLTEFEKAIGYKNPDRPSPDIVRS"
     gene            complement(1024..>1205)
                     /locus_tag="EYO86_03530"
     CDS             complement(1024..>1205)
                     /locus_tag="EYO86_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42135.1"
                     /translation="PLMGWETSKDTMSEVKLEFSTKDQAINYARSNNIDYYIIEPQKR
                     KIIKKSYSDNFLKNN"
BASE COUNT          407 a          229 c          171 g          398 t
ORIGIN      
        1 agcttaatta taagatccat tttacgatct tacgatatct ggacttggtc tatcaggatt
       61 cttgtaacct atagcttttt caaattcagt aagccgtcta aatattgatc ttaattcggt
      121 aattcttgtc cagttttgca tgaataatga gaagctgccc tgcacttctc taaacgcact
      181 tgaagtttgt attagtatcc ctaaagtcat tgcgcctgta aataaaccag gtcccattat
      241 tatgtaagga acgattatca ttgattggtt atacataatt aaccataaat caaaataacc
      301 gtagtgaaga aatagccgat gataattgaa tttaattcca gtaaaaagtt ctaatactgt
      361 tggaggtttt gcatactcgg ccctgtcgtc ttctccataa acgagttctt ttctaaaagc
      421 tgcttctact acctgattat tgtattccaa gcctggaagt ttaattccaa ccagccaact
      481 aattaagaca ccacccaagc tagctgtaag agaaacatat actaatgaac cacttatatc
      541 ctttagccaa ggcaagataa cattagatga caatccccaa agaataggaa taaaagctat
      601 cagtaacata aaagctctta ttatttgaag acctatactt tcaacaattc tggcaaaatt
      661 catacaatcc tcttgaattc gttgagaggc accttcaact ttagcatcaa cttccttcca
      721 ataaggcata tatgcaaaag tcatagcttc tctccaccga aaggcataca gacgagtaaa
      781 ataattagtg attgtagaga ttataacata aggaatagct aacttcatga acaggaaaat
      841 tccatcataa aactctgaca catcgctttc agttgccttt tgtaaaaggt cgtaaaatcc
      901 cttgtaccat tgattaattt ttacatcgat ccaggtttga gctattaaag atccgataat
      961 aaataaaaaa cctccccaag cccacaagcg ccattttttg ctaaagaaaa aacttttaat
     1021 catttaatta ttttttaaaa agttatcaga atatgatttc ttaatgattt tacgtttttg
     1081 tggttcaata atataataat caatattatt acttcttgca taatttattg cttgatcttt
     1141 tgttgaaaac tctaatttaa cttctgacat ggtgtctttt gatgtctccc aacccattaa
     1201 aggat
//
LOCUS       DTVV01000297            2866 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1875535, whole genome shotgun sequence.
ACCESSION   DTVV01000297 DTVV01000000
VERSION     DTVV01000297.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1875535"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            108..1181
                     /locus_tag="EYO86_03535"
     CDS             108..1181
                     /locus_tag="EYO86_03535"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:PRK07559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HIC42136.1"
                     /translation="MLSGSLTYKDYDDLYNKGQIINPYFLKSQIQPSSVDLTLSEECY
                     EINASFLSPKTNVRDKLSQILVKKIDLNERFVFEKNKTFLVKLNESLNLQDSIFGLCN
                     PKSTTGRLDIFCRTVLNNSDEYEKIPINYQGEMFIEITSRSFNLELQKGDSLNQMRLI
                     SVKHIYLDDSDLQKYHNENYLTLNDKNIKIQPNISCGLKLSVDLSHKNITNAYVAKHN
                     APNLCFQKVRFHKTSDYWNSIKTQNGTIIIEKNNFYILKSKEKIHIPKNMAGEMIPYD
                     TGLGDFRVHYAGFFDPGFGNLNGSFAVLEVKTNEVPFLLEDGQIIARIKYEMLNKDSD
                     VVYGTDINSNYQNQSLALSKHFV"
     gene            1193..1633
                     /gene="aroQ"
                     /locus_tag="EYO86_03540"
     CDS             1193..1633
                     /gene="aroQ"
                     /locus_tag="EYO86_03540"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIC42137.1"
                     /translation="MKKIILINGPNLNILGNRENNIYGNHSLEEIKKQSEEKSAELNL
                     ECFFCQSNNEGELINFIHSVEKEYDGLIINPAAFTHTSVALLDALRSISKPKIEIHLS
                     NIYTREEYRKKSITSEGVDGLICGFGSLSYILAIEAINNLIYTK"
     gene            1638..2087
                     /locus_tag="EYO86_03545"
     CDS             1638..2087
                     /locus_tag="EYO86_03545"
                     /inference="COORDINATES: protein motif:HMM:PF00364.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIC42138.1"
                     /translation="MSKIDKTDLENITLIKKELKLKNVTSVKIKKGDYEIELSSQNQP
                     LSLNPATSVASPKNPSTNTEKIEKSPSSENIVKSPMVGVAYLSVDPSTPPYVKVGQHV
                     KQGETLLIIEAMKTFNEVKAPKSGIVKKIIVLNSQPVEFGDDLLIFE"
     gene            2087..>2866
                     /locus_tag="EYO86_03550"
     CDS             2087..>2866
                     /locus_tag="EYO86_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIC42139.1"
                     /translation="MFKKELIANRGEIALRILRACRELNIKTVAIHSEADESAMHVRM
                     ADESVCVGPASAQSSYLNIPAIITAATITNVDAIHPGYGFLSENQRFAEIIEEHNVKF
                     IGPNSQHIKMMGNKISARKIMKDNNVPIVPGLNDISDKNKIKQFIDRTELPIIIKAAS
                     GGGGKGMRIIKDYSELDKSINAAKLEAKKSFNDDTVYIEKFLTSPKHIEIQILSDSHG
                     NVVTLGERDCSIQRKHQKLIEESPSTILTQKERDQISELCRK"
BASE COUNT         1127 a          404 c          441 g          894 t
ORIGIN      
        1 aaattataat attgaagaaa taatgaaaaa aatgttaatt ctcttaaaaa atacaaatga
       61 tcctattctg caaacactaa taagaaaaga tattaagaaa attaataatg cttagtggtt
      121 cactaacata taaagattat gatgatcttt acaataaagg acaaataatt aatccatatt
      181 ttttaaagtc ccaaatacaa ccatcaagtg tggatcttac tcttagtgaa gaatgttacg
      241 aaataaatgc aagtttttta agccctaaaa caaatgttcg tgacaaatta tctcaaattc
      301 ttgtaaaaaa aattgatctc aatgaaagat ttgtatttga aaaaaataaa acatttctag
      361 taaaattaaa cgaaagtttg aatttacaag acagcatttt tggattgtgt aaccctaaaa
      421 gtaccacagg tcgtctagac attttttgtc gaacagtatt aaataatagt gacgaatatg
      481 aaaaaattcc aataaactac caaggagaaa tgtttataga aataacttct cgctcattta
      541 atttagaact gcaaaaaggt gattctctta atcaaatgcg tttaatttct gttaaacata
      601 tttaccttga tgatagtgat cttcaaaaat atcataatga aaattaccta accttaaatg
      661 ataaaaatat aaaaattcaa cccaatattt cttgtggcct taagctctca gttgatctat
      721 cacataaaaa tataactaat gcttatgttg ctaaacataa tgctcccaat ctatgttttc
      781 aaaaagtccg atttcataaa acttctgatt attggaattc aattaaaaca caaaatggaa
      841 caattattat tgaaaaaaat aatttttata ttttaaaatc aaaagaaaaa atacatatac
      901 ctaaaaatat ggcaggtgag atgattcctt atgatactgg gcttggcgat tttcgtgttc
      961 attatgctgg tttttttgac cctggttttg gtaacttgaa tggctctttt gcggtacttg
     1021 aagttaaaac aaacgaagtt ccatttcttt tagaggatgg gcaaataata gctagaataa
     1081 aatatgaaat gcttaacaag gattccgatg tggtatatgg tactgatatt aattccaact
     1141 atcaaaatca gagtcttgca ttaagcaaac actttgttta gaatttaaaa taatgaaaaa
     1201 aatcatccta attaatggtc caaatttaaa cattcttggt aatcgggaaa ataatattta
     1261 tggtaatcat tccttagaag agatcaaaaa acagtcagaa gaaaaaagtg ccgagcttaa
     1321 tcttgagtgt tttttttgtc agagtaacaa tgaaggtgaa ctcattaatt ttattcattc
     1381 agtggagaaa gaatatgatg gattaataat taatccagct gcttttactc acacttcagt
     1441 tgctcttcta gatgcactta gatctatttc aaaacccaaa attgaaattc atttgtccaa
     1501 tatatacaca agagaagaat atagaaaaaa aagcattaca tcagaaggag ttgatggatt
     1561 aatatgtggt tttggttctc ttagttatat tctagctata gaagccatta ataatttaat
     1621 ttatactaaa taattatatg tcaaaaattg ataaaacaga tctagagaat attactttaa
     1681 taaaaaaaga attaaaatta aaaaacgtaa ctagtgtaaa gataaaaaaa ggagattatg
     1741 aaattgaact ttcttctcaa aatcaaccac tttctttaaa tcctgctaca tctgttgctt
     1801 ctccgaaaaa cccttcaaca aatactgaaa aaatagaaaa aagtccaagt tcagagaata
     1861 ttgttaaatc accaatggtc ggggtagctt atttgtcggt tgacccttcc acacctccct
     1921 atgtaaaggt tggacaacat gttaaacaag gagaaacttt actgatcatt gaagccatga
     1981 aaacttttaa tgaagtcaag gcaccaaagt caggtatcgt aaaaaaaatt attgttctta
     2041 atagtcaacc tgtggagttt ggtgacgatc ttctaatatt tgaataatgt tcaaaaaaga
     2101 acttatcgct aatagaggcg agattgctct acgtatttta cgggcgtgta gagagcttaa
     2161 tattaaaaca gtagctatac actcagaagc tgatgaatct gcaatgcatg tccgaatggc
     2221 tgatgaaagt gtatgcgttg gacctgcctc agcacaatca agttatttga atatccctgc
     2281 aattattact gcagcaacaa taactaatgt tgatgctata catccaggat acggattttt
     2341 aagcgagaat caacgatttg ctgaaatcat tgaagagcat aatgttaaat ttattggccc
     2401 taattcccaa cacataaaaa tgatggggaa taaaatttct gctaggaaaa taatgaagga
     2461 taataatgta cctatagtgc caggattaaa tgacatctct gataaaaata aaattaaaca
     2521 atttattgat agaacagaac tgccaataat aattaaggct gcaagtggtg gcggtggaaa
     2581 aggaatgaga ataattaagg attatagtga attagataaa tcaattaacg ctgcaaaatt
     2641 agaagcaaaa aaatcgttta atgatgatac tgtttatatt gaaaaatttt taacatctcc
     2701 caaacatatt gaaatacaaa tattatctga ttctcatggc aacgtagtaa ctttaggtga
     2761 aagagattgt tctattcaac gcaaacatca aaagcttatt gaggaaagtc cgagtacaat
     2821 tttaacacaa aaagaacgtg atcaaattag tgaactttgt cgaaaa
//
LOCUS       DTVV01000298            1857 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1877449, whole genome shotgun sequence.
ACCESSION   DTVV01000298 DTVV01000000
VERSION     DTVV01000298.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1877449"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="EYO86_03555"
     CDS             <1..165
                     /locus_tag="EYO86_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIC42140.1"
                     /translation="DIFTVPASLAGIPGISIPFGKSQNGLPLGIQLLSKHFDEQLVLN
                     AGLYLEKNNV"
     gene            158..1624
                     /gene="gatB"
                     /locus_tag="EYO86_03560"
     CDS             158..1624
                     /gene="gatB"
                     /locus_tag="EYO86_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIC42141.1"
                     /translation="MSSLIQGKFSKWETVIGLEIHAQIKSNSKLFSSSSTKFGSSPNS
                     QVSLFDAAMPGMLPVINEFCIKQAVKTGLGLKAKINRYSVFDRKNYFYADLPQGYQIS
                     QYKHPIIGEGAVTIDFKDGSSKDIRIVRLHLEQDAGKSLHDQNPSKTYVDLNRSGVAL
                     MEIVSEPDLNSSHEASLYITKIRSILMYLDTCDGNMQEGSLRTDVNISVRKPGEELGT
                     RCEIKNLNSIKFIKQAIEYEAMRQIKIIEEGGIIEQNTLLFNPQLGKTKPMRSKEEAH
                     DYRYFPDPDLLPLKLEDDFIEIIKKDIPELPDERKKRYINEYKLSNYDSSVLTIEKSI
                     SDYFDKVLGCHQDLKKSAKLVVNWITSELFAILKKNEVEIHNSPITPYNLGQLVKLII
                     SNKISGKIAKDVFEDMYKNSLSPEEIVNSRGLAQVTNVKEIELIIDQILKLNYDKVND
                     YKAGKTKLLGFFVGQAMQISKGKANPKILNDILIKKLS"
BASE COUNT          716 a          257 c          283 g          601 t
ORIGIN      
        1 gatatattta ccgtaccagc ttctcttgct ggtataccag gcatttcaat tccttttggt
       61 aaatctcaaa atggtcttcc tttaggcata caattgttat ctaaacactt tgatgaacaa
      121 ttagttttaa atgctggatt gtatttagaa aaaaacaatg tctagtttaa ttcaaggaaa
      181 attttctaaa tgggaaactg taattggttt agaaatacat gctcaaataa aatctaattc
      241 caaattattt agttcctctt ctacaaaatt tggctctagt ccaaatagcc aagtctcttt
      301 attcgatgct gcaatgccag gaatgcttcc agttataaat gaattttgta ttaagcaagc
      361 tgttaaaaca ggattaggat taaaagcaaa aataaaccga tattcagttt ttgatcgaaa
      421 aaattatttt tatgcagatc ttccccaagg ttatcaaatt agtcaatata agcacccaat
      481 aataggtgaa ggagctgtga caattgattt caaagatggt tcttcaaaag atataagaat
      541 tgtaagatta catttggagc aggatgctgg aaaaagttta catgatcaaa atccttctaa
      601 aacttacgtt gatttaaata gatcaggggt agctttaatg gaaattgtta gtgaacctga
      661 tttaaacagc tctcacgagg catctcttta tattactaag attagatcta tactcatgta
      721 tctagataca tgtgatggca atatgcaaga aggatccctt aggactgatg ttaacatctc
      781 agttagaaaa ccaggtgaag aacttggaac taggtgtgaa ataaaaaatt taaattcaat
      841 caaatttatt aaacaagcta ttgaatatga ggcaatgaga cagatcaaaa ttattgaaga
      901 aggaggtata attgagcaaa ataccttgtt gtttaatcct caattaggaa aaacgaaacc
      961 aatgagaagc aaagaagaag cccatgatta tagatacttt cctgatcccg atttactccc
     1021 attaaaactt gaagatgatt ttatagaaat tattaaaaaa gatattcctg aacttcctga
     1081 tgaaagaaaa aaaagataca ttaatgaata taaattatcg aattatgatt cttcagtttt
     1141 aacaattgaa aaatcaatat ctgattattt tgataaagtt ttgggttgtc atcaagattt
     1201 aaaaaaatca gctaaacttg ttgttaattg gattacttct gagttatttg caattttaaa
     1261 aaagaatgaa gttgaaattc acaattctcc aattactcct tataatcttg gtcaattagt
     1321 taaattaata atctctaata aaatttcagg aaaaattgca aaagatgttt ttgaagatat
     1381 gtataaaaat agtctatcgc cagaggaaat tgtaaattct cgaggattag ctcaagttac
     1441 aaatgttaaa gaaattgaac tgataataga tcaaatatta aagctaaatt atgataaagt
     1501 gaatgactac aaagcaggta aaactaaatt attaggtttt tttgtaggtc aagccatgca
     1561 aatcagtaaa ggtaaggcaa atccaaaaat acttaatgat attctaatta agaaattatc
     1621 atgatcaaaa gaaaattaat cttaatgtaa aaattctata agataaaaaa atcaaagtta
     1681 atactaccca aataaaacta cctttatatt tttctgcaga tcgccatatc ccaacagtta
     1741 tatatatagt ccaaattatt agtattattt tgtttcgaat gtttatgacg gattcaataa
     1801 acaccctaac ggaaaccgcg cacgtttcat atcatttcag ctattgaaac ctggtga
//
LOCUS       DTVV01000299            1174 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1880511, whole genome shotgun sequence.
ACCESSION   DTVV01000299 DTVV01000000
VERSION     DTVV01000299.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1880511"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            64..750
                     /locus_tag="EYO86_03565"
     CDS             64..750
                     /locus_tag="EYO86_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42142.1"
                     /translation="MSCFVLGNPNEPVYSGEIQCKGLGMDIDIFDEQGKSITNKKGEL
                     VCKSPFPSKPLFFWNDKNNQKFNEAYFIKYPNQWHHGDYAKITVNDGLIIYGRSDATL
                     NSGGVRIGTAELYRVVENIENVVECIAVEHQLVNDTQVILFLKLIPNKLINDEFVLKI
                     RNQIKYLLSPKHIPSKMIQVQDIPKTKNGKIVELTVKKIMNNEAVLNLNSLVNPECLL
                     EYKKIAAELN"
     gene            798..>1174
                     /locus_tag="EYO86_03570"
     CDS             798..>1174
                     /locus_tag="EYO86_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HIC42143.1"
                     /translation="MSKKIVISAIGGPEVLKYIDYDLPSNLQKDHVRIRQTSIGVNFI
                     DTYHRSGIYPLPSKPPVCPGLEAAGEIIDLGTEVEDFNIGDRVCYAAVPLGAYCEIRD
                     FPSSKIIKIPNYISADTAASILL"
BASE COUNT          432 a          163 c          191 g          388 t
ORIGIN      
        1 tacgtttata aatttattaa aagaaatatt catttagctt ctataagtgg agggacagat
       61 attgtgtcat gcttcgtttt aggtaatcca aatgaaccag tatattcagg agaaattcaa
      121 tgtaaaggtc taggtatgga tattgatatt tttgatgaac aaggaaaatc tataacaaat
      181 aaaaaaggtg agctagtgtg caaatctccc ttcccttcaa aacctttatt tttttggaac
      241 gataaaaata atcaaaaatt caatgaagct tattttatta aatatcccaa ccaatggcat
      301 catggagatt atgcaaaaat aactgtaaat gatggcttga tcatttatgg tcgttccgat
      361 gccacgttaa attctggggg ggttagaata ggtacggctg aattgtatag agtcgttgaa
      421 aatatagaaa atgttgttga atgtattgca gttgaacatc aacttgtaaa tgatactcaa
      481 gttattttat ttctcaaact aattcctaat aaattaataa atgatgaatt tgttttaaaa
      541 ataagaaatc aaattaaata tttactatca cccaaacaca taccttctaa aatgatacaa
      601 gtgcaagata tcccaaaaac aaaaaatggt aagatagtag aattaacagt aaaaaaaata
      661 atgaataatg aagctgtttt aaatctaaac tccttggtta atccagaatg cttgttagaa
      721 tataaaaaaa tcgcagccga attaaattaa cgaatttcta attgcaaaaa tagtaaaata
      781 tatgtacctt gattattatg tcaaaaaaaa tagtaatttc agcaattggt ggacctgaag
      841 ttttaaaata tattgattat gatttgcctt ctaatcttca aaaagatcat gttcgtataa
      901 ggcaaacttc tattggcgta aattttattg atacatacca tcgtagcgga atttatccac
      961 ttccctcaaa acctcctgtt tgccctggat tagaggcagc tggagaaatt attgatttgg
     1021 gtactgaggt tgaagatttt aatattggag atagagtttg ttatgctgca gttcctcttg
     1081 gggcttattg tgaaataaga gactttcctt catccaaaat tattaaaatt ccgaactata
     1141 tctctgcaga tacagcagca tctattttac tgca
//
LOCUS       DTVV01000300            1594 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1882810, whole genome shotgun sequence.
ACCESSION   DTVV01000300 DTVV01000000
VERSION     DTVV01000300.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1882810"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="EYO86_03575"
     CDS             complement(<1..546)
                     /locus_tag="EYO86_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIC42144.1"
                     /translation="MNLIDYAISHARVFFGILIFIIAAGSSTYISIPKEAVPDVNIPI
                     IYVSLSQKGISANDSERLLVKPIEDEVKTVEGIKEIRSTAYTGGGNVLLEFDAGFDAD
                     KAMDDIREKVDRAKGDLPNEADEPTVNEVNISAFPIILISLSGNLPDRTLQDLAENLQ
                     DEIETIPSVLEAKIGGKRNEQV"
     gene            complement(557..1594)
                     /locus_tag="EYO86_03580"
     CDS             complement(557..1594)
                     /locus_tag="EYO86_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIC42145.1"
                     /translation="MDQSIVIQGQTVYNKKIDVKSEITGNITNINFDRGDKVQVNKNL
                     LNISIENRKEVLASVQKDLERLNKGLIINEKNRDNLLSKNAELIKLYEIEYLSAKQLI
                     DKGLSSKSKLSLASFNLANAKSDQIDINLKYETQLANLESQIASYQSQLKQINLDIEN
                     TQISAPFDGIITNKNIEISDYVTPGNILLTIVNLNPIKVQGYLSESDVNKVQLYTKAT
                     IENSNGIKKNGKITFISPSAETSTRTFEIEIEADNSDLSFKSGITTSIMIEGSELLAH
                     KIPPSILTLQDDGTIGVKALDENNTVIFYPIQKVKDTIDGMWVSGLPNEINLIISGQE
                     YVSSGQIIELK"
BASE COUNT          495 a          249 c          211 g          639 t
ORIGIN      
        1 aacttgttca ttacgtttcc caccaatttt agcttctaga acacttggaa ttgtttcaat
       61 ctcatcttga agattttctg caagatcttg tagagttcta tctggtaaat ttccacttaa
      121 agaaattaaa atgataggaa atgcgctaat attaacttca tttacagtcg gttcatcagc
      181 ttcattgggt aaatctcctt tggctctatc tactttttct ctaatgtcat ccattgcctt
      241 atctgcatca aatccagcat caaattctaa aagtacattg ccgcctcctg tatatgctgt
      301 tgatcgtatt tctttaattc cctctactgt cttaacttca tcttcaatag gttttacaag
      361 caatctttca gaatcattag ctgatattcc tttttgagat aaggaaacat aaattattgg
      421 tatattaaca tcaggtactg cctctttagg aatagaaata taagttgaag atcctgccgc
      481 tatgataaaa atcaaaatac caaaaaaaac tcttgcatgc gaaattgcat aatcaattaa
      541 gttcatttct ttacttttat tttagttcta ttatttgacc tgaagaaaca tattcttgac
      601 cagaaataat caaattgatt tcatttggca atccactaac ccacatacca tcaatagtat
      661 ctttaacttt ttgtattgga taaaagatta cagtattgtt ttcatctaaa gcttttacac
      721 caatggtacc atcatcttgt aaagttaaaa tagaaggggg tattttatga gcaagtagtt
      781 ctgagccttc aatcataatt gaagttgtga taccactttt gaatgataaa tcagaattat
      841 cagcttcaat ttcaatctca aaggttctag tgcttgtttc agcagatggt gaaataaatg
      901 taattttacc atttttttta attccattgc tattttctat tgttgctttt gtatataatt
      961 gtactttatt gacatcagat tcagataaat atccctgaac tttaataggg tttagattaa
     1021 caatagttaa taaaatattt ccaggagtaa catagtcact aatttcaata ttcttattgg
     1081 taattattcc atcaaaagga gcagatattt gcgtgttttc tatatctaga tttatctgtt
     1141 ttaattgtga ttgataagaa gcaatttgag attctaaatt agctaattga gtttcatatt
     1201 ttaagttaat atctatttga tcagattttg catttgctaa attaaaggat gctaaactta
     1261 attttgattt tgaacttaga cctttatcta taagttgttt tgcagaaaga tattcaattt
     1321 catatagttt tatgagctct gcatttttgg ataataagtt atcacggttt ttttcattga
     1381 taattagtcc tttattaagt ctttccaagt ctttttggac actggctaat acttcttttc
     1441 tattttcaat tgagatattt aaaagatttt tatttacttg aactttatcc cctctatcaa
     1501 aatttatatt ggtaatattc cctgtgattt cagattttac atcaatcttt ttattataaa
     1561 ctgtttgtcc ctgaataact attgattgat caat
//
LOCUS       DTVV01000301            1828 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1883174, whole genome shotgun sequence.
ACCESSION   DTVV01000301 DTVV01000000
VERSION     DTVV01000301.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1883174"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="EYO86_03585"
     CDS             <1..510
                     /locus_tag="EYO86_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC42146.1"
                     /translation="ANYFFSERLGILYSFFHNLWGFNWYDNIHDAYIYVFIAATWKQI
                     SANFIFFLAGLQTIQKPIIEASMIDCKSSFRRFWTITFPLLMPTTFFLIIINITYSFF
                     DTLGIIDTTTIGAPSGETKTLVFKAYIDGFRGLDLGSAGAQSIVMMLIVLVITVFQFR
                     FIEGRIHYN"
     gene            510..1334
                     /locus_tag="EYO86_03590"
     CDS             510..1334
                     /locus_tag="EYO86_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC42147.1"
                     /translation="MSRTISQLLSHFILIAGMLLMVVPIWIIFASSTHDNITILSDGM
                     KWGLGDQLIKNYDEVLNQKGGFSQEITATKLFLNSFIMALGIATLTVTTSLMSAYAIV
                     YFRFKLAVPLFWLIFITLLVPLELRILPSYQVVSDLHLINTYSGLILPLSASAIATFF
                     FRQFYKSVPDELLEAAKLDGANSWKFFIDFLIPLSKTMMAAMFIFMFVYGYNQYLWPL
                     IMTTSEKYWTVVMGMKIIFMASTVERFAYIILSIIPPVLVVVFLQRWFIQGLFQTK"
     gene            1331..>1828
                     /locus_tag="EYO86_03595"
     CDS             1331..>1828
                     /locus_tag="EYO86_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate ABC transporter permease"
                     /protein_id="HIC42148.1"
                     /translation="MNKKIAQFSAHTVLFLGVLLMVVPIWIAFASSTHENVTILKEGM
                     KWGLGDQLVKNYNEVLNQKGGFSQEITATKMFINSFIMAFGIASIKVIISSMSAYAIV
                     YFRFKLAVPLFWLIFITLLVPLEVRILPSYQVVSDLHLTNTYTGLILPLVASATATFF
                     FRQFYK"
BASE COUNT          545 a          300 c          315 g          668 t
ORIGIN      
        1 gcgaattatt ttttcagtga aagattagga attttgtata gtttttttca taatctttgg
       61 ggttttaatt ggtatgacaa tatacatgat gcttacattt atgtttttat tgcagcaact
      121 tggaaacaaa taagcgcaaa ttttattttc tttttagcag ggctacaaac cattcaaaaa
      181 ccaatcattg aagcgtccat gattgattgt aaaagtagct ttagaagatt ttggactatt
      241 acttttcctt tattaatgcc aacaaccttt ttcttgataa ttattaatat tacttactct
      301 ttttttgata cacttggaat aatagataca actactatcg gagcgcctag tggcgaaacc
      361 aaaacacttg ttttcaaggc ttatattgat ggttttaggg gacttgatct tggaagtgct
      421 ggcgcccaat caatcgttat gatgttgatt gttttagtaa ttacagtttt tcaatttaga
      481 ttcattgaag gaaggatcca ttataattaa tgagcagaac aatttctcaa ttattatctc
      541 actttatttt gattgccggg atgcttctaa tggttgttcc aatctggatt atctttgcta
      601 gttctactca tgacaatatc acaattctat ctgatggaat gaagtggggt ttaggcgatc
      661 aattgataaa aaattatgac gaagttttaa accaaaaagg cggcttttcc caggagatta
      721 cagctacaaa attgtttctt aatagtttta taatggcttt aggaattgca actcttacag
      781 taactacttc attaatgtcg gcatatgcaa ttgtatactt taggtttaaa cttgctgtac
      841 ctcttttttg gttaatcttc attacccttc ttgtgcctct cgagttaagg atattaccga
      901 gttaccaggt agtttcagat ctacatttaa taaatactta cagtggattg attttaccac
      961 tatctgcatc agccatagct acttttttct tcaggcaatt ttacaaatct gtccctgatg
     1021 aactcctgga agcagcaaag ttagacggcg caaacagttg gaaatttttc atagattttt
     1081 tgatcccgtt atcaaaaaca atgatggcag caatgttcat tttcatgttt gtatatggtt
     1141 acaatcaata cttatggcct ttgattatga ctacttctga aaaatattgg accgtcgtaa
     1201 tgggaatgaa aattatcttt atggcaagta cagtggagag atttgcctac atcatattat
     1261 caattatacc tccagtattg gttgtggttt ttttacagcg ctggtttatt caaggattat
     1321 ttcaaacaaa atgaataaaa aaatagctca attttctgcg catacagtcc tctttctagg
     1381 ggtattattg atggtagttc caatttggat tgcttttgcc agttcaacac acgaaaatgt
     1441 aaccatttta aaagaaggaa tgaaatgggg tcttggagac caattagtca aaaattataa
     1501 tgaagtttta aatcaaaaag gcggtttttc ccaggagatt accgctacga aaatgttcat
     1561 caatagtttc atcatggctt ttggaattgc gagcataaaa gtaattattt catccatgtc
     1621 cgcgtatgca attgtatact ttaggtttaa acttgccgta cctctttttt ggctaatatt
     1681 tataacgctg ttagttccat tggaagtaag aattcttcca agctatcaag ttgtatctga
     1741 tctacattta acaaacactt acactggctt gattctccca ttagttgcgt ccgctacggc
     1801 tacatttttc ttcaggcagt tttacaaa
//
LOCUS       DTVV01000302            1030 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1887654, whole genome shotgun sequence.
ACCESSION   DTVV01000302 DTVV01000000
VERSION     DTVV01000302.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1887654"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
BASE COUNT          354 a          168 c          169 g          339 t
ORIGIN      
        1 tatggagctg tgttagttca aaattcagat ttatttgaat cttatcaaag cttttatact
       61 gaaatgaata ccaaattaga aaaaaagttt ttctccatgg ttactggtta ttcagcagct
      121 cctgaaagtt cttggctttc tagactaagt ttttcaaccg gcacaaagat ggatcacaga
      181 tacatagcta acctagcaat ggtgtccaat ttgacgacat taaataagaa attttcttta
      241 gaaggtcata aaacttttag ttttatgcca ggtgtcactg aacatgttga atacatagaa
      301 tcattcttcg atttcgaaaa aagaattcgt ttttggcatt tagattggcc tgctgatata
      361 aatccagttg gctgggcagt cattccagat caatttgctt tggagtggtt gggaagaaaa
      421 ttaagtttcg agaacttagc ccctttttac gcagagatag tcctctcaag cagccataca
      481 ccatttggcg aagtgcctcc ttacatacca aaaggaaatt ttaacttaga agactatcct
      541 ttaattaaaa aaagaaattc tttgggtatt ttgtctcaca tggaaagcag ccctaaaaaa
      601 tacatgattt caattgaata tagtctcaat tcagtttgtg actttattgg aaaatatctt
      661 agcccggaag atttgattat tgtaatagga gatcatcaac ctcctgtact gaaaactaat
      721 tctaatctag ttccagtaca tattattagt ggtaaaaaga gcttattaga cccatttgaa
      781 aaaataggtt tccataacaa tttatctcaa ttaaaactta aaaaacatat taatcacgaa
      841 gacattgcgg aattcctatt ggagaactat tcttcttatt aagtaaggaa aaaatgaata
      901 aaattaataa cgtaaaaaat gctattaaag tttcttcgtt cattcagtct agagatcttg
      961 atcttgacaa aactacacga gaattgtggc cagctatact agccttaaaa aatttcttgg
     1021 tttctgctaa
//
LOCUS       DTVV01000303            2408 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1892362, whole genome shotgun sequence.
ACCESSION   DTVV01000303 DTVV01000000
VERSION     DTVV01000303.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1892362"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="EYO86_03600"
     CDS             complement(<1..630)
                     /locus_tag="EYO86_03600"
                     /inference="COORDINATES: protein motif:HMM:PF05977.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC42149.1"
                     /translation="MLSKISSYVNFKSINSSVKYLSISALFTGIALGYFFTLIVILAK
                     YKGYTEGTIGIIAASFSLGLISAGFIVSNILDKIGLYKTMSLAILIQTICVILMFSFF
                     NPLNLAINHFIMGVFGGMIWMTMDTWVNLVSDNNNRGKAIGFYNSAITIGFAIGPLFV
                     GFFGVKGIIPITLAISLMFIRSPILILIKKQVENVQIPKLDKKINFSFIK"
     gene            complement(637..2013)
                     /locus_tag="EYO86_03605"
     CDS             complement(637..2013)
                     /locus_tag="EYO86_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIC42150.1"
                     /translation="MKNDYISEIHEKDKLVLHPYEDLKDRGTYNRKLIVKSDNIYLYD
                     ENGKRYIDGPGGMWCNNIGHGNKEIAEAIKNQIETMDYCSPFSESSEKAAELASVLSE
                     LSPGDLNTVFFTPDGSSANETALRFMMLYNNILGRPEKKHIISRDRAYHGSTYLTGSI
                     TGKDREKNYFDFESTFIHHVGSPNPYRRKDNQTVDDFCDEKVKEFEDKILEIGPNKVA
                     AYIAEPVLASGGCIVPPKNYIKRFWEICKKHDILYISDEVVTGFGRLGHFFCSQEEFG
                     IIPDIITSAKGITSGYIPLGAVLISDRLMKSISKDSSLFFHGFTYSGHPVSCAAALKN
                     IEIIKRDKILDHVKNIAPYFHQKLKTLYEIPIVGDVRGMGLMAGIECVIDKDSKNPLI
                     LDQAIASRIDEESLKLGLIIRPIYHICVLSPALIIQKEQVDDLVDKLYQAISNATKKL
                     EKEGLWSG"
     gene            complement(2095..>2408)
                     /locus_tag="EYO86_03610"
     CDS             complement(2095..>2408)
                     /locus_tag="EYO86_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037436.1"
                     /note="catalyzes the dehydration of L-carnitinyl-CoA to
                     crotonobetainyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carnitinyl-CoA dehydratase"
                     /protein_id="HIC42151.1"
                     /translation="EFLMTGRWMDVKEAKHWGLINEIVPSKDLLKRAREIANNIAKGP
                     NLIYSSIKEVLRETENVNEQPAFQTLRSLESVKKVYGSKDLVEGATSFVKKSSPEWKG
                     E"
BASE COUNT          775 a          432 c          364 g          837 t
ORIGIN      
        1 tttaataaaa gaaaaattta tttttttatc aagcttaggt atttgtacat tttcaacttg
       61 ttttttaatc aaaattaata ttggtgaacg tataaacatc agactgattg ctagggtgat
      121 aggtataatt ccttttacac caaaaaaacc aacaaataat gggcctatag caaaaccaat
      181 tgtaattgct gaattataaa aaccgatagc tttacctctg ttattattat ctgaaacaag
      241 attgacccat gtgtccattg tcatccaaat cattcctcca aaaacgccca ttataaaatg
      301 attaattgct agattcagag gattaaaaaa actgaacatt agtatcacac aaatagtttg
      361 tataaggatt gcaagtgaca ttgttttata caaaccaatt ttatccaaga tgtttgaaac
      421 aataaaacca gcagaaatta accccaaaga aaaactggca gcaattatgc cgatagtgcc
      481 ttcggtataa cctttgtatt ttgccagaat aactattagt gtaaaaaaat accccagtgc
      541 tattcccgtg aataaggctg agattgataa atattttaca ctagagttta tgcttttaaa
      601 atttacataa ctacttattt tagatagcat atatagctag cccgaccaca aaccttcttt
      661 ttctaatttt ttcgtagcgt tggaaatcgc ctggtataac ttgtcaacta aatcatctac
      721 ttgttctttt tgaattataa gagcagggga taaaacacaa atatgataaa taggtcgaat
      781 tattaaacct agtttaagtg attcttcatc tatgcgcgaa gctattgcct gatctaatat
      841 tagcggattt ttgctatctt tatcaataac acattcaatc ccagccatga gtcccatgcc
      901 acgcacgtcc ccaacaatgg gtatttcgta taatgttttt agcttttgat gaaaataggg
      961 cgcaatattt ttaacatgat caagaatttt atcacgttta ataatttcaa tgttctttaa
     1021 agcagcagca cacgatactg gatgacccga atatgtaaaa ccatgaaaga ataaagagga
     1081 atccttgctt atacttttca ttaatctatc tgaaattaaa acggctccta gagggatata
     1141 tcctgaagta attccttttg cagaagttat aatatccgga ataatgccaa attcttcttg
     1201 cgagcaaaag aaatgtccaa gtcttccaaa tcctgtaact acttcgtcag aaatgtacag
     1261 aatgtcatgc tttttacata tttcccaaaa acgtttaata tagtttttcg gaggaacaat
     1321 gcaaccacca gatgcaagaa caggttctgc aatgtatgct gcaactttat ttggacctat
     1381 ttctaatatt ttatcttcaa actcttttac tttttcatca caaaaatcat caactgtttg
     1441 attatctttt ctcctatagg gatttgggga accaacatga tgaataaaag tggattcaaa
     1501 atcaaaataa tttttttccc tatcttttcc agttattgat cccgttaagt atgtgcttcc
     1561 gtgataggct cgatctcgag aaataatatg ttttttctcc ggccttccta aaatgttgtt
     1621 ataaagcatc ataaagcgca atgctgtttc attggcagag gaaccatcgg gggtaaaaaa
     1681 tactgtattt aaatcaccag gtgatagctc cgataagaca gaagctaatt cagctgcttt
     1741 ttcggatgat tctgaaaaag gactacagta atccatggtt tcaatttgat ttttaatagc
     1801 ctccgcaatt tctttatttc catgaccgat attattacac cacatgcctc caggaccatc
     1861 aatgtagcgt ttaccatttt catcgtataa gtaaatgttg tctgacttga cgattaattt
     1921 tctgttgtaa gtccccctat ctttgaggtc ttcataggga tgcagaacaa gtttatcttt
     1981 ttcatgtatt tcgcttatgt aatcattttt cattgaccaa atgtatcatt tgctgaatat
     2041 gcattcaatg aaaatagggg aatttgttag tttttttaat taattaaaaa aaaattattc
     2101 acctttccat tctggtgaac tttttttaac aaaagacgtt gcgccttcta ctagatcttt
     2161 actgccataa acttttttta cagattctaa cgagcgtaaa gtttgaaaag ctggttgctc
     2221 atttacattt tctgtctcac gtaaaacttc tttgattgat gagtaaatta aattgggccc
     2281 tttagcaata ttgtttgcaa tttctcttgc tctttttagt aaatctttgc tgggaacaat
     2341 ttcatttatt aagccccaat gttttgcctc tttaacgtcc atccaacggc ctgtcatgag
     2401 aaattcta
//
LOCUS       DTVV01000304            1352 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1893972, whole genome shotgun sequence.
ACCESSION   DTVV01000304 DTVV01000000
VERSION     DTVV01000304.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1893972"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="EYO86_03615"
     CDS             <1..320
                     /locus_tag="EYO86_03615"
                     /inference="COORDINATES: protein motif:HMM:PF01810.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42152.1"
                     /translation="FVEEQQHQFNSFLQGFVIAIINPKILIWFTAIYSQFINIHANII
                     FNTTLVLTASIIDALWYILVSFLVTGYGLKNFLIERKKMIQKITGSLLILIALSLLYK
                     IIS"
     gene            complement(313..>1352)
                     /locus_tag="EYO86_03620"
     CDS             complement(313..>1352)
                     /locus_tag="EYO86_03620"
                     /inference="COORDINATES: protein motif:HMM:PF01244.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42153.1"
                     /translation="GEDITQRTDVGHSDLVRLRESCIDLEILVVWASKTPPEGSAFVI
                     ANAMYDAIEALATKNDFLTIPKTLNDITISKKNNQLSIPIAMEGGEALENSLEKLHHF
                     IERGVFYLGPTWNYSLDWVSSGYDEVHNKKNIKHFGLSKFGQEVIHTCNDNGVMIDVS
                     HVGEKSFWDIAAISKKPFIASHSSVYKLCTHFRNLKDDQILEIKKSQGLIGLNPYPFF
                     IDSSFKKKEREFLKQFKNELDQITHKQPNPTASWIAKQHYLQKKLEDIVPLLDIFIDH
                     IEYIIKLIGVDYVGIGSDYDGLDCLPQGWKDCIDHIKIAEALESRGYSVEDIEKVMGR
                     NILRVLDLVAN"
BASE COUNT          415 a          263 c          205 g          469 t
ORIGIN      
        1 actttgtaga ggaacagcaa catcagttta attcatttct tcagggcttt gtcattgcta
       61 tcattaatcc aaaaatttta atttggttta cggctattta ttctcaattc ataaacatcc
      121 acgccaatat tatatttaat acaaccctcg ttttgacagc ttcaattatc gatgccctat
      181 ggtatatcct cgttagcttt ctggtaacag gttatggact taaaaatttt ttaattgaac
      241 gcaaaaaaat gatccaaaag ataactgggt ctttattaat tcttattgct ttgagccttc
      301 tttacaaaat aattagttag ccactagatc aagaacacgt aaaatatttc gacccataac
      361 tttttctata tcttctactg agtatcccct gctctccaaa gcttcggcta ttttgatgtg
      421 atcaatgcag tccttccatc cctgaggcaa gcaatccaat ccatcataat cactgcctat
      481 tcccacataa tcaacaccaa ttagcttaat aatatattca atatgatcga taaatatatc
      541 taacaaagga acaatatctt ctagtttttt ttgtaaatag tgttgtttgg caatccagga
      601 ggcagtcggg ttcggttgtt tatgagtaat ttgatccaat tcgtttttga attgctttaa
      661 aaattctctt tcttttttct taaaagaaga atcaataaaa aaaggatagg ggtttaagcc
      721 tattaaacct tgtgattttt taatctctaa aatttgatca tcttttaggt ttctaaaatg
      781 cgtacaaagt ttgtaaacgc tagaatgaga ggcgataaaa ggtttcttgg atattgctgc
      841 tatatcccaa aaacttttct ctccgacatg ggacacatca atcatcaccc cgttgtcatt
      901 gcaagtatga atgacttcct gtccaaattt acttaatcca aaatgcttaa tatttttttt
      961 attgtgtacc tcatcataac ctgagctaac ccaatccaat gaataattcc atgttggtcc
     1021 aagataaaac acccctcttt caataaaatg atggagcttc tccaaagaat tttctaaggc
     1081 ctccccgcct tccatggcga taggaatgga caattgatta ttttttttgc ttatagtgat
     1141 atcatttaaa gttttaggta ttgtaagaaa gtcatttttt gttgctaggg cttctattgc
     1201 atcgtacatt gcattggcaa tcacaaatgc gcttccctcc ggtggagtct ttgaagccca
     1261 gacaactaag atttctaaat caatgcagga ttcacgtaaa cgaacaagat ctgaatggcc
     1321 cacatcagtg cgttgagtaa tgtcttcacc aa
//
LOCUS       DTVV01000305            1849 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1897184, whole genome shotgun sequence.
ACCESSION   DTVV01000305 DTVV01000000
VERSION     DTVV01000305.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1897184"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="EYO86_03625"
     CDS             complement(<1..351)
                     /locus_tag="EYO86_03625"
                     /inference="COORDINATES: protein motif:HMM:PF00581.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42154.1"
                     /translation="MRNVNDIKKNNIQSVNSRQCFERLSKVSDSHLVDVRTKPEWLFV
                     GLPDLQSLQKKTVCVSWHVYPDMEINVNFESGILDSGINKQDTVFLICRSGQRSSDAA
                     DYLASRGFTNCYNVI"
     gene            555..1205
                     /locus_tag="EYO86_03630"
     CDS             555..1205
                     /locus_tag="EYO86_03630"
                     /inference="COORDINATES: protein motif:HMM:PRK00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIC42155.1"
                     /translation="MKIKFYGHAGVKIEDNLVLLIDPWLNDNPIATVKTDDITKADYI
                     IATHNHFDHVNDIPLIAKNTGATVVGIVETAEDLASKGCEKIIGCNIGGIVKLNGLEL
                     LFTHAFHSMSSNPSGVIIFYKNKTIYHAGDTGVFSDMKLIGEMYPIDLAFLPVGGHFT
                     MGLKEAQKAIQLLNAKKIVPIHYNTFDLIKQNVDENLVKESNSELLILEPGQETDI"
     gene            complement(1217..>1849)
                     /locus_tag="EYO86_03635"
     CDS             complement(1217..>1849)
                     /locus_tag="EYO86_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stearoyl-CoA 9-desaturase"
                     /protein_id="HIC42156.1"
                     /translation="TASTKDPHDFEIVISKPTDVLYFFSFFIPLANVFYLHKSLQFET
                     LQHSLGITTNVLRECVPEREINKCRASARIHVSIWIISAGASLYFNSWLPLLYIVLPV
                     FYGNTLRVAFGLTQHSGLQENIKDHRYSTRTVILNPIFSFLYWHMEYHIEHHMFPTVP
                     SYNLPKLHAMIKDQTPPAKKGFWGAYSEIIPAIIKQSKDPNYKISLSVPN"
BASE COUNT          610 a          286 c          342 g          611 t
ORIGIN      
        1 aatcacatta tagcaattag taaagccacg ggatgctagg tagtccgcag catcggaaga
       61 tcgttgacct gaacgacaaa ttaaaaaaac agtatcctgc ttattaattc cagaatctaa
      121 aattccggat tcaaaattta cattaatctc catatcggga tatacatgcc aagaaacaca
      181 cacagttttt ttttgcaaag actgtaaatc tggtagacct acaaataacc attcgggctt
      241 ggttcttaca tcaaccaaat gtgaatcaga cactttagac aatctttcaa aacattgccg
      301 tgaattgaca ctttgaatat tatttttttt aatgtcattt acattccgca tggcgaaggt
      361 cctcccacaa gctgtgcatg ttcttgattt tacgatctgt atggaattat tttctaaata
      421 tacaattatt atttatatta tttagaatat tttttgtgag attagaaatt tttagaatat
      481 ttttctaaat ttactaaata tcaaataatt attttatctc aacattctat gttgttggtg
      541 gtaatgtaat ctagatgaaa ataaaatttt atggacatgc gggtgtaaag attgaggata
      601 atttggtttt gttaattgat ccttggttaa atgataatcc tatagcgaca gtgaaaacag
      661 acgatattac caaggctgac tatattattg caacgcacaa tcattttgat catgttaacg
      721 atataccttt gattgcaaaa aatactggag ctacggttgt tggtattgtg gaaacggcgg
      781 aagatttggc atcgaaaggt tgtgaaaaaa taattggttg caatattggg ggaatagtca
      841 agttgaacgg tttagaattg ttatttactc atgctttcca ttccatgagc tccaatccaa
      901 gcggcgtgat cattttttat aaaaacaaaa caatatacca tgcgggcgat acgggagtat
      961 ttagtgatat gaaattaatt ggtgagatgt atcccattga tttggccttt cttcctgtag
     1021 gcggtcattt tacgatgggt ttaaaagagg cccaaaaagc cattcaatta ttgaatgcaa
     1081 aaaagattgt ccctattcac tacaatacat ttgacctgat taaacagaat gttgatgaga
     1141 atcttgtaaa agaatctaac tcagagttat taatcctgga gcctggtcaa gaaactgaca
     1201 tctaatcata tacttatcaa ttgggtacgc ttaagctaat cttataatta ggatctttgg
     1261 actgtttaat aattgccggg ataatctcac tatatgcccc ccaaaatcct tttttggctg
     1321 ggggtgtttg atcttttatc atagcatgta atttgggtaa gttgtaagat ggaactgtgg
     1381 gaaacatatg atgttcaata tgatattcca tatgccaata taaaaaacta aaaattggat
     1441 taagaattac tgttcgagtg ctatacctgt gatctttaat gttttcttga agaccagagt
     1501 gctgagtgag tccaaaagca accctaagtg tgttcccata gaagactgga agaacgatat
     1561 acaatagtgg aagccatgaa ttaaagtaga gtgatgctcc tgcagaaatt atccaaatac
     1621 ttacgtgaat gcgagctgat gctctgcact tatttatttc tctttcagga acacactccc
     1681 tcaaaacatt tgtagttatt cctagtgaat gttgtaaagt ttcaaattga agagatttat
     1741 ggagataaaa aacatttgct aatggaataa aaaatgagaa gaagtaaagt acatccgttg
     1801 gtttgcttat aacaatttca aaatcgtgcg gatcttttgt agatgctgt
//
LOCUS       DTVV01000306            1013 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1900701, whole genome shotgun sequence.
ACCESSION   DTVV01000306 DTVV01000000
VERSION     DTVV01000306.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1900701"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1013
                     /locus_tag="EYO86_03640"
     CDS             <1..>1013
                     /locus_tag="EYO86_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIC42157.1"
                     /translation="VDALFDNGRKGRAILGSNKRPLKSLSEMLKGKQGRFRQNLLGKR
                     VDYSGRSVIVPGPHLLLHQCGIPKKMALELFKPFIYCKLEQYGLSNTIKTSKKLVEKE
                     RPEVWDVLEEVIKEHPVLLNRAPTLHRLGFQAFEPLLIEGKAIQLHPLVCTAFNADFD
                     GDQMAVHVPLSLEAQLEARVLMMSTNNILSPSSGKPIIVPTQDMVLGIYYLSLMFEDE
                     IGSNMAFTDIAEIEHALLAKKISLHSKIKCRYQFLNEENKEESSIAETTAGRMIIYEL
                     LPKHPKIGLDLINKILTKKQVSNVIDAIYRHCGQTETVKFCDKIMVLGFEHACRAGIS
                     FGK"
BASE COUNT          380 a          134 c          185 g          314 t
ORIGIN      
        1 cagttgatgc tctttttgac aatggtagaa aaggaagagc aatattgggt tcaaataaaa
       61 gaccacttaa atctttatct gagatgttaa aaggtaaaca aggtagattt agacaaaact
      121 tactgggaaa aagagttgat tattcaggta gatctgtaat agttcctggc ccacatttat
      181 tattacatca atgtggtatt cctaaaaaaa tggctttaga actatttaaa ccatttatat
      241 attgtaaatt agaacaatac ggcctttcca atacaataaa aacatcaaag aaattagtag
      301 aaaaagaaag accagaagtg tgggatgttt tggaagaagt tataaaagaa catccagtat
      361 tattaaatcg tgcgccaact ttgcataggc ttggattcca agcatttgaa cctttgttaa
      421 tagaaggaaa agcgattcaa ttacatccat tagtttgtac agcttttaac gctgattttg
      481 atggtgatca aatggcagtt catgtacctc tgtcattaga agctcaactt gaagctcgtg
      541 ttttaatgat gtccacgaac aatattttga gtccatcaag tggaaaacca ataattgttc
      601 ctactcagga catggttttg ggaatttatt atttgagcct aatgtttgaa gatgagatag
      661 gatcgaatat ggcctttact gatattgcag aaatagaaca tgctttatta gcaaaaaaaa
      721 tttcattaca ttctaaaatt aaatgtagat atcaattttt aaatgaagag aataaagaag
      781 aaagttctat agctgaaact acagctggga gaatgataat ctatgaactt ttaccaaaac
      841 atccaaaaat aggattagat ctaataaata aaattctaac aaaaaaacaa gtaagtaatg
      901 ttatagatgc aatttataga cattgtgggc aaacagaaac tgtcaaattt tgtgataaaa
      961 ttatggtttt aggttttgag cacgcgtgca gagcaggtat ttcttttggg aaa
//
LOCUS       DTVV01000307            1222 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1903711, whole genome shotgun sequence.
ACCESSION   DTVV01000307 DTVV01000000
VERSION     DTVV01000307.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1903711"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1222
                     /gene="infB"
                     /locus_tag="EYO86_03645"
     CDS             <1..>1222
                     /gene="infB"
                     /locus_tag="EYO86_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIC42158.1"
                     /translation="KKSTLKPQDDEFGKINVNKILEQEEQEYDKFPSLAKLKRARIKE
                     KLKLQENPEENHVGREITIPEIITVQDLANRMAEKTADVVKTLMKMGVMANATQSLEA
                     DTAEIVATELGYRVKIVNDDDILKDIEDFKDPATAMKKRAPIVTIMGHVDHGKTSILD
                     VFRSSNVVDGEKGGITQHIGAYQINNNNNIITFIDTPGHAAFSNMRARGSKTTDIIIL
                     VVAADDGIKPQTVESIAHAKSANVPIIVAINKIDLPSADPDKVRNELLNHGIIVEKLS
                     GEVLDVEISALKKINFNKLEEAIHLQADLLNLKANPNRIARGVVIETKLEKGRGSVAT
                     ILIQKGTLKIGDIFVSGSEWGKVKALINSNGENVKEALPSMPIEVLGFDANPLAGDDF
                     VVVDNENSARKIAEH"
BASE COUNT          487 a          162 c          236 g          337 t
ORIGIN      
        1 aaaaaaatct acccttaaac ctcaagatga tgaatttggt aaaatcaatg ttaataaaat
       61 tttagaacaa gaagaacaag aatacgataa gtttcctagt ttagcaaaat taaaacgtgc
      121 tagaattaag gaaaaattaa aattgcaaga gaatccagaa gaaaatcatg ttggaagaga
      181 aataactatt ccagaaatta ttacagtaca agatttagct aacagaatgg cagaaaaaac
      241 tgctgatgta gttaaaacat taatgaagat gggtgttatg gcgaatgcta cgcaatcttt
      301 agaagcagat acagcggaaa ttgtagcaac agaattaggc tatagggtta aaattgtaaa
      361 tgatgacgat attttaaaag atattgaaga ttttaaagat cctgcaacag ctatgaaaaa
      421 aagagcccca attgtaacta taatgggaca cgtggatcat ggaaaaacta gtattttaga
      481 tgtttttaga agttctaatg ttgtagatgg agaaaaagga ggcattactc aacatattgg
      541 cgcttatcaa ataaataaca ataataatat aattacattt atagataccc ccggtcacgc
      601 agctttttca aatatgcgtg cacgaggctc aaaaacaaca gatattataa ttctagttgt
      661 tgctgcggat gatggtatta aaccccaaac agttgaatca atagcacatg ctaaatctgc
      721 aaatgttcca ataattgtgg caatcaataa aattgaccta ccatcagcag atccagacaa
      781 agttagaaat gagcttttaa atcacggaat aattgttgaa aaattaagcg gtgaagtact
      841 tgacgttgaa atttcagcat taaaaaaaat taattttaat aagttagaag aagccataca
      901 cttacaggct gatttattaa atttaaaagc aaatcctaat agaattgcga gaggagtggt
      961 tattgaaaca aaacttgaaa aaggtagggg ttcggtagca actattctta tacaaaaagg
     1021 cactctaaag attggtgaca tatttgtatc tggttcggag tggggaaaag taaaagcatt
     1081 gattaatagt aatggagaaa atgttaagga agcccttcca tcaatgccaa ttgaagtctt
     1141 aggctttgat gccaatcctc tagcgggaga tgattttgtt gttgttgata atgaaaatag
     1201 tgcaagaaaa atagcagagc at
//
LOCUS       DTVV01000308            1353 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1905785, whole genome shotgun sequence.
ACCESSION   DTVV01000308 DTVV01000000
VERSION     DTVV01000308.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1905785"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="EYO86_03650"
     CDS             complement(<1..671)
                     /locus_tag="EYO86_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIC42159.1"
                     /translation="MMNYTFHWYPAFKLLPKMLYGSITTIEIAFLSIIFGIILGAILA
                     LAKNSNNKIIFSLANTWIEISRNTPALYQIYMAYFGLGTFGIYVGSFGALLIGITFNN
                     SGYLAETFRGGLRAIPATQMKTGRSLGMNFFQSFIYIIFPQLFRIVFHSVTNQTVLAI
                     LMTSLGVIVGMNTDLMGVTNELNALSFRTFEYFLQFLTNISLSFLQLQINLKLRAFSS
                     SFVSRG"
     gene            complement(754..1353)
                     /locus_tag="EYO86_03655"
                     /pseudo
     CDS             complement(754..1353)
                     /locus_tag="EYO86_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007113593.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
BASE COUNT          471 a          234 c          208 g          440 t
ORIGIN      
        1 cctcgagaga caaaggagga agagaatgct cgcaatttaa gattgatttg tagttggagg
       61 aatgataatg atatattagt tagaaattgt aaaaaatatt caaaagttct aaatgataaa
      121 gcattaagct cattcgtaac tcccattaag tctgtattca ttccaacaat cacacctaaa
      181 gaggtcatta atattgccaa gacagtttga ttggtgactg agtgaaatac aattctaaac
      241 aattgtggaa agataatata aataaaagat tgaaaaaaat tcattcctag tgatcttcct
      301 gttttcattt gagtagctgg tattgccctt aggccacctc taaacgtttc cgctaagtaa
      361 ccagaattat taaaagttat gccaataagt aaagcaccaa aagaaccaac atagattcca
      421 aatgttccaa gaccaaaata agccatgtaa atctgatata atgctggtgt gttcctagag
      481 atttctatcc atgtattagc tagagaaaaa ataattttat tattactatt ttttgcaagt
      541 gctagaatag cgccaaggat tattccaaat attattgata ggaatgctat ttcgattgta
      601 gtaattgagc catagagcat tttaggtaaa agcttaaatg ccggatacca atgaaaagta
      661 taattcatta ttttgtataa tatacgttca aaagataaag gatgattaat ctgttaatca
      721 tcctttataa ttactagtta ttaacctttg atattaatag taagttcctg gaattgtata
      781 atcaggcatt tttccttcac caatccattt attaaataat tcttgcgctc tacctgttct
      841 aatttgttga ttaacaaaaa gattaagata gttcaacaat ccatattcat gtctcaaagc
      901 aatcaaacta acatagtcag tatcatatgg agctgtgccc tcaactgata aagttggata
      961 cttcccagaa ttcacatgag cccaagcaac ggtactagtt acaactgttg cttcaatttg
     1021 tttttgctta agtgctaaca acaaatctgc ttcactttga taggcactaa atgagccacc
     1081 acagccttca acaaatggtt caagtcggat tgcctcataa gtaccagcta catttcctac
     1141 ttttttacca cacatatcgt cataagtttt aatgcctgaa tcttttcttg ttagaacatt
     1201 aaactcaaat gcaaaatatg gaattgtaaa cccaatagtt ttagctcttt ctaatgtatc
     1261 agatgttgaa gctataccaa cgtctgccct acccgatacc agtgctggta ttctatcagg
     1321 aaaaggtgtt tacacaattt taatttttac aca
//
LOCUS       DTVV01000309            3316 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1906443, whole genome shotgun sequence.
ACCESSION   DTVV01000309 DTVV01000000
VERSION     DTVV01000309.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1906443"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /gene="hisH"
                     /locus_tag="EYO86_03660"
     CDS             <1..264
                     /gene="hisH"
                     /locus_tag="EYO86_03660"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:PF00117.26"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIC42160.1"
                     /translation="KFPHMGWNNVKIRESFPKMIFSKENKDFYFVHNYYFECLNKKNI
                     IGSTKYGLNFASIIGKENIYGVQFHPEKSSVQGLQLIKNFLSI"
     gene            261..797
                     /locus_tag="EYO86_03665"
     CDS             261..797
                     /locus_tag="EYO86_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIC42161.1"
                     /translation="MNINAKEKINIKVETLQSVSDVDLADLCNITEHAIRAGGGFGWL
                     KVPTRDVLKKYWNGLVVVQNRILVVGRLNDAIAGALQLGLQSPNNEAQKNIATITSHF
                     VVPWARGYGLAKNMIDHAETVARESSIVCIQLDIRETQEAAIQLFKSKGYEQWGTNPS
                     YAQVEGNLIKGLYFYKKI"
     gene            776..1528
                     /locus_tag="EYO86_03670"
     CDS             776..1528
                     /locus_tag="EYO86_03670"
                     /inference="COORDINATES: protein motif:HMM:PF00977.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HIC42162.1"
                     /translation="MFLQKNLVILIPAIDLKNNKCVRLIRGKEEEQTIFNENPVKQAM
                     FFEESGCERLHVVDLDGAFGKPNINKETILKIRKKTNIPIELGGGIKNKEDVYFWLNK
                     GIDFLIVGSLAVKKSNLILDLASQFENQIYVALDVLKGNIMIKGWVESSEFTINDIFE
                     VYDTSQINGYILTDISRDGMLQGLDMNLIKKYAAITKKNLIVGGGLSNYKDLRNLNII
                     SYTNLEGVIAGKSYYSGAIKIDKALKILKSNA"
     gene            1521..2276
                     /gene="hisF"
                     /locus_tag="EYO86_03675"
     CDS             1521..2276
                     /gene="hisF"
                     /locus_tag="EYO86_03675"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIC42163.1"
                     /translation="MLKIRIIPCLDVKHGRVVKGINFVNLIDAGDPVEQARFYSDNGA
                     DEICFLDIGASLEKRKTMIDVVAKTSERVFIPLTVGGGINSIQDIRGLLKAGADKISI
                     NTAAILNENLIAESSSRFGSQCIVAAVDVKKYYEDWIVYSHGGTKNTGINAMSWLEKL
                     QQLGAGEILLTSMDKDGTKDGFDLGLLKRANDILNIPVIASGGVGSLKHFEEGVTIGG
                     ASALLAASVFHFAEFSILDVKRYLQKQNIQVRF"
     gene            2277..2576
                     /gene="hisE"
                     /locus_tag="EYO86_03680"
     CDS             2277..2576
                     /gene="hisE"
                     /locus_tag="EYO86_03680"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIC42164.1"
                     /translation="MNKKKETDFLQELFNIIQNKAKGKDNKSYTKFLLKSGKKKIAKK
                     VGEESVELLIDYLNGTKKRTIEEASDLIYHLFVLLYSKKISLNDIKKELTNRHHV"
     gene            2569..2919
                     /locus_tag="EYO86_03685"
     CDS             2569..2919
                     /locus_tag="EYO86_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011477487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HIC42165.1"
                     /translation="MYDKNNIFAKILRKEIPCEVVYEDDQVLFFYDINPQAKVHILGI
                     PKKKVVDFRDFILRLDQSTVQYFFTKIIDVINGLGLDQKGYRLIINNGRDANQEVPHF
                     HIHILAGENLKGIR"
     gene            complement(2921..>3316)
                     /locus_tag="EYO86_03690"
     CDS             complement(2921..>3316)
                     /locus_tag="EYO86_03690"
                     /inference="COORDINATES: protein motif:HMM:PF04972.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIC42166.1"
                     /translation="SENRLFLDVNSNVTEGRVLLTGLVDTQEIRIEAVRKVWEINGVR
                     EVMNEIEVGNKTTLKEYMNDLWINTQVKSLAARTIGLRSFSYNFETIKGKVYIAGITS
                     RPEQLQAIVESTKTIKGVNEIVNYVIIKE"
BASE COUNT         1222 a          438 c          599 g         1057 t
ORIGIN      
        1 aaatttccac atatgggctg gaacaatgtc aagatacgag aaagttttcc aaaaatgatt
       61 ttttcaaaag aaaataagga tttttatttt gtgcacaatt actattttga atgtttaaat
      121 aaaaaaaata taattggatc aaccaaatat ggactaaatt ttgcttctat aattggcaaa
      181 gaaaatatat atggtgttca gtttcatcct gaaaaaagct cagtacaagg attgcaatta
      241 ataaaaaatt ttttatctat atgaatataa acgcaaaaga aaaaattaac ataaaggttg
      301 aaaccctcca atcagtttca gacgtggatc ttgcagacct ttgtaatatt acagagcatg
      361 ctattagggc cgggggcggt tttggctggc ttaaagttcc aacaagagat gttcttaaaa
      421 aatattggaa cggcttagtt gttgttcaaa ataggatact tgtagtgggc agattaaatg
      481 atgctattgc aggagcatta cagttgggct tgcaatcacc aaacaacgaa gcacaaaaaa
      541 atatagctac aataacatct cattttgttg ttccttgggc aagaggatat ggtttggcaa
      601 aaaatatgat tgatcatgct gaaactgtag cgagagaaag cagtattgtt tgtattcagc
      661 ttgacattcg agaaactcag gaagccgcta ttcagctatt taaatcaaaa ggatatgagc
      721 aatggggaac gaatcctagt tatgctcaag ttgaaggaaa tttaattaaa ggtttatatt
      781 tttacaaaaa aatttagtga tactcattcc agcaatagat cttaagaaca acaagtgtgt
      841 tagactaatc agaggaaaag aagaagaaca aacaatattt aatgaaaatc cagtcaaaca
      901 agcaatgttt tttgaagaga gtggttgtga aagattacat gttgttgatc ttgatggggc
      961 ttttggtaag cccaatataa acaaagaaac aattcttaaa attagaaaaa aaacaaacat
     1021 tcctattgaa ctcggaggag gcataaaaaa taaagaagat gtttattttt ggttaaacaa
     1081 aggcattgat tttttaattg ttggttcgct tgcagtaaaa aaaagtaatt taattttaga
     1141 tttagccagt caatttgaaa atcaaatata cgttgcactc gatgtgttga agggcaatat
     1201 aatgatcaaa ggttgggttg aaagttcaga atttacaata aatgatattt ttgaagttta
     1261 tgatacaagt cagataaacg gatatatttt aacagacatt tcaagagacg gtatgctaca
     1321 aggtttagat atgaatttaa taaagaaata tgcggcaatt actaaaaaaa atctaatagt
     1381 tggtggcggt ctctcgaact ataaagatct tagaaattta aatataattt cttacactaa
     1441 tttagagggc gttattgctg gaaaatcata ttattcggga gctatcaaga tagataaggc
     1501 tttaaaaatt ttaaagtcca atgcttaaaa taagaattat tccttgtttg gatgttaagc
     1561 atggaagagt cgttaaagga attaactttg ttaatttaat tgatgcggga gaccctgtcg
     1621 agcaagctag attttattca gataatggcg cagatgagat atgtttttta gatatcggag
     1681 cttcactgga aaaaagaaaa acaatgattg atgttgttgc aaaaacatct gaaagagttt
     1741 ttatacccct aacagttgga ggtggaataa attctattca agatattagg ggtttgctga
     1801 aagctggcgc agataaaatt tctattaata cagctgcaat tttaaacgaa aatttaatcg
     1861 ctgaatcttc tagtagattt ggcagtcagt gcatagtggc tgcagtagat gtaaaaaaat
     1921 attatgagga ttggattgtg tattcacatg gcggaactaa aaatacagga attaatgcaa
     1981 tgtcatggtt agaaaaattg caacaattag gcgccggtga aatattatta acctcaatgg
     2041 acaaagatgg cactaaagat ggttttgacc taggtcttct taagagagct aatgatattt
     2101 tgaatatacc tgtcatagct agtggagggg taggaagttt aaaacatttt gaggaaggtg
     2161 tgaccatagg aggcgccagc gctcttttag cggcttctgt ttttcatttt gctgaattta
     2221 gtatattgga tgttaaacga tatttacaaa aacaaaacat acaagtaaga ttctaaatga
     2281 acaaaaaaaa agaaactgat tttttgcaag aattgtttaa tattattcaa aacaaagcaa
     2341 aaggaaaaga taataagtct tatacaaaat ttttattaaa gagtggaaaa aagaaaatag
     2401 caaaaaaagt tggtgaagaa agtgtagaac tattaataga ttatttaaac ggcactaaaa
     2461 aaagaactat agaagaggct tccgatttga tttatcactt gtttgtatta ctatactcaa
     2521 aaaaaatcag tttaaatgat ataaaaaaag agttaacaaa tagacatcat gtatgataaa
     2581 aataatatat ttgccaaaat tttaagaaag gaaattccct gtgaggttgt gtacgaagat
     2641 gatcaggtat tattttttta tgatattaac cctcaagcta aagttcatat tttaggaata
     2701 cctaaaaaaa aagtcgtgga ttttagagat tttatcctca gattagatca atcaactgtc
     2761 caatattttt tcactaaaat tatagatgta attaatggtt tgggattgga tcaaaaagga
     2821 tacaggctta ttatcaacaa tggtagagat gcaaatcaag aagtccccca ttttcacatt
     2881 catatcttgg ctggagagaa cttaaaagga atcagataaa ttattctttt ataattacgt
     2941 aattaacgat ttcgtttaca cccttgattg ttttagtgct ttcgacaata gcctgaagtt
     3001 gttcaggcct acttgtgatg cctgcgatat aaaccttacc ttttattgtt tcaaagttat
     3061 aactaaaact tcttaagcca attgttctgg ccgcaagtga ttttacttga gtattaatcc
     3121 atagatcatt catatattcc ttaagtgtcg ttttattgcc aacttctatt tcattcataa
     3181 cctcccttac accatttatt tcccaaactt ttcttactgc ttcaattcta atttcttggg
     3241 tgtcaactaa accagtaagt aacacccttc cttctgtgac attactattt acatctaaaa
     3301 ataatctatt ttcaga
//
LOCUS       DTVV01000310            2342 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1909115, whole genome shotgun sequence.
ACCESSION   DTVV01000310 DTVV01000000
VERSION     DTVV01000310.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1909115"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="EYO86_03695"
     CDS             <1..844
                     /locus_tag="EYO86_03695"
                     /inference="COORDINATES: protein motif:HMM:PF13480.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIC42167.1"
                     /translation="QSAIPFTPVTGERIIVSDALKDKLSKKVEIINRIIKKTKKINVS
                     SLHFNFLPQPNNIKQQNPELLVRQGIQFHWKNNNYKSFDDFLKTLSSRKRKVIKKERL
                     CIDSNNLKIKLLIGDKITSEHWDFFYQCYLNTTGRKWGSAYLTKDFFLELSKTLADKI
                     LLIIALKEEKMVASAINFLSSTHLYGRLWGTMYDIPYLHFELCYYQAIEYAIANNIKI
                     VEAGAQGEHKLQRGYMPEKIWSLHWIKDQQFSKAINKYLDQEISLTNKQKKDLEQFAP
                     FKNN"
     gene            complement(841..>2342)
                     /locus_tag="EYO86_03700"
     CDS             complement(841..>2342)
                     /locus_tag="EYO86_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIC42168.1"
                     /translation="TRYRGDFEERLKAVLNELRKEKKAILFIDEIHTVIGAGATSGGS
                     MDASNILKPSLGKGDLRCIGSTTYKEFTNYFEKDRALVRRFQKIDIKEPTKDEAIKIL
                     KGLKNYYEKHHGIKYSTDAIISAVELSNKYIGDRKLPDKAIDVIDEAGASQYLLPVSS
                     RKKIITSKEIEAVVALIARIPTKSVSSNDKEGLKNLERDLKNFVFGQDIAIKELASSI
                     KLARAGLREEGKPIGSYLFSGPTGVGKTEVARQLSKTLGIKLLRFDMSEYLERHSVSR
                     LIGAPPGYVGFDQGGLLTDGIDQHPHSVLLLDEIEKAHFDLFNILLQIMDYGKLTDHN
                     GKSVDFRNVILIMTTNAGAVDISKKRIGFNSVRSSSDSSEAIDKLFTPEFRNRIDSII
                     HFNHLNKEIVLSIVDKFIIEVEAQLYEKGVTLAIDQETKEYLADKGYDEVYGARQLSR
                     VMQEEIKKPMAEELIFGSIAKGGHIDIILKDNKVNFQYSHKQEEKKVLV"
BASE COUNT          740 a          461 c          357 g          784 t
ORIGIN      
        1 gcaatcagca atccctttta cccccgtcac gggggagaga attattgtca gtgatgcgct
       61 aaaagataaa ttgtcaaaaa aagttgaaat cattaatagg attatcaaaa aaacaaaaaa
      121 aataaatgtt tcctccctac attttaattt tcttccccaa cccaataaca taaagcaaca
      181 aaatccagag ttgttagtac gacaaggaat tcaatttcat tggaaaaata ataattacaa
      241 atcttttgat gattttctta aaacactgtc ttctcgtaaa agaaaagtga ttaaaaaaga
      301 gcgtctttgc attgatagca acaatctaaa aataaaatta ttgatcggtg acaaaattac
      361 ttctgagcat tgggattttt tttatcagtg ttatcttaac acgactggaa gaaaatgggg
      421 ttctgcctac ctaacgaaag attttttttt agaacttagc aagacactcg cagataaaat
      481 tcttctcatt attgcgctta aagaagaaaa gatggttgct tcagccataa attttcttag
      541 ctcaactcat ttatatggaa ggctatgggg gacaatgtat gatattccct acctgcattt
      601 tgaattatgt tattaccaag caattgaata tgctattgca aacaacatca aaattgttga
      661 agcaggcgca caaggagaac ataaattgca aagagggtac atgcctgaaa aaatatggag
      721 tttgcactgg atcaaagatc aacagtttag caaagcaatt aacaaatatt tggatcaaga
      781 aattagccta acgaacaaac aaaaaaaaga tttggagcaa ttcgcaccct tcaaaaacaa
      841 ctaaaccaaa acttttttct cttcttgttt gtgtgaatat tgaaaattaa ctttattatc
      901 ttttagtatg atatcaatgt gccctccttt agcgatggaa ccaaaaatta actcttcagc
      961 cattggtttt tttatttctt cctgcatcac cctgctaagc tgtcttgcac cgtatacttc
     1021 atcatatcct ttatcagcca aatattcttt agtttcttga tcaatagcta gggttacacc
     1081 tttttcatat agttgtgcct ccacctcaat aataaattta tcgacaatcg atagcactat
     1141 ttctttattc aaatgattga aatgaataat tgagtcaatt ctatttctaa actctggtgt
     1201 aaataatttg tctattgctt cactgctgtc actacttgac ctcacagaat taaatcctat
     1261 tcttttttta gatatatcaa ctgcccctgc atttgttgtc atgattagta tcacgttgcg
     1321 aaaatctaca gatttcccat tatggtctgt tagtttgccg taatccataa tttgcaaaag
     1381 aatattgaaa agatcaaaat gagctttttc aatttcatcc agcaataaaa cgctatgagg
     1441 gtgctggtcg atgccatctg tgagcaagcc accctgatcg aaacccacat accccggagg
     1501 agctccaatt agacgactta ccgaatggcg ctccaaatat tctgacatgt caaatcgaag
     1561 taatttaatt cctagtgttt tacttaattg ccttgcaacc tctgtttttc caactccggt
     1621 tggaccagag aacaaataag agccgatggg ttttccttct tctcgtaagc ccgctcttgc
     1681 aagcttgata gaagaggcaa gttccttaat tgctatatct tgaccaaaaa caaaattttt
     1741 caaatctctt tctaggtttt tcaagccctc tttatcattg ctactgacac tttttgttgg
     1801 aatacgcgct atcaacgcaa caactgcttc aatttctttg gatgttataa ttttctttcg
     1861 tgaggaaaca ggtagcaaat actgagaggc tcctgcttca tctataacat ctatagcctt
     1921 atccgggagt ttgcgatcac caatatattt attcgataat tcgacagcgc tgataattgc
     1981 atctgttgaa tacttgattc cgtggtgttt ctcatagtaa ttttttaaac cttttaatat
     2041 ttttatagcc tcatcttttg taggctcttt aatatctatt ttctgaaaac gtcttacaag
     2101 tgctctgtct ttttcaaaat aatttgtaaa ctctttgtag gttgtcgacc caatacatcg
     2161 tagatccccc ttacctaaag aaggttttaa aatattgcta gcatccatcg atccgccact
     2221 agttgcccca gctccaatga cagtatgtat ctcatcaata aaaagaattg cttttttttc
     2281 ttttcttaac tcgtttaaaa cagcttttaa gcgttcctca aaatctcccc gatatcttgt
     2341 gc
//
LOCUS       DTVV01000311            2834 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1910922, whole genome shotgun sequence.
ACCESSION   DTVV01000311 DTVV01000000
VERSION     DTVV01000311.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1910922"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="EYO86_03705"
     CDS             <1..679
                     /locus_tag="EYO86_03705"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622852.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HIC42169.1"
                     /translation="NIMFAGGGEELHWTMSILFDAMGALSSKYNETPHLASRAYDTNR
                     DGFVISGGGGTIVLEELEHAKARGAKIYAEITGYGATSDGHDMVQPSGEGAVRCMNQA
                     LKTMDGEIDYINSHGTSTPIGDLKELEAIKKVFPNKSPHISSTKSLTGHSLGATGVHE
                     TIYSLLMLNNNFLCGSANISDLDESAKEHNILIETINKKDIQNVISNSFGFGGTNATL
                     GFSKINA"
     gene            672..1448
                     /locus_tag="EYO86_03710"
     CDS             672..1448
                     /locus_tag="EYO86_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC42170.1"
                     /translation="MLKNKKGIIFGIANDHSIAWGIAKKLSEKGAEFGITYQNEILLK
                     RVKPLADKVNSSLLIECDFSKTESIKSCFEEIKAKFQNIDFVIHAAAFSDKNELNGKY
                     INTSKDNFIKTLNISCFSFTEISKYASEIMNDGGSLLTLSFYGSNKVMPNYNVMGVAK
                     AALETSVKYLSVDLGNKNIRVNAISAGPMRTLAGAAIANARDVYNYTQANSALIRNVK
                     LEEIGNAALYFVSDLSNAVTGEVHYVDCGFNILGMPKSNT"
     gene            complement(1445..2188)
                     /locus_tag="EYO86_03715"
     CDS             complement(1445..2188)
                     /locus_tag="EYO86_03715"
                     /inference="COORDINATES: protein motif:HMM:PF03009.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42171.1"
                     /translation="MLNPPKLIGHRGVKNLSPENTLNSIELAHKLGLKWIEIDVKISK
                     DLVPILLHDDTLERTTDGKGLPTDFNYDDLKKLDAGFSFYNYSTNICIPTLKQVLLFC
                     KKNEICLNIELKPNLGFEKNNVEAIAKLLSNFNFTNQYYFSSFDWPSVILMKKFLPNS
                     YYGLLIDGFSKDISLKNTLDICEKYNFSCCGFNKNIINSDIMNEMKRHNLITTIYSEK
                     NLKPSEANALWSMGVKSIFIDDPKEFKIS"
     gene            complement(2196..>2834)
                     /locus_tag="EYO86_03720"
     CDS             complement(2196..>2834)
                     /locus_tag="EYO86_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIC42172.1"
                     /translation="DEDHLGDMDFKVAGTKDGITSLQMDIKITSITSDIMKEALVQAK
                     EGRLHILGEMAKAIDKSRDSISDYAPTITTIQVHKDKIREVIGKGGAVIREITEKTGA
                     KIEINDVGLVSIAAVDRESGQAAIDWINSIVEEPEIGKIYEGKVVKLMDFGAFVNFMG
                     SRDGLVHISQLKNERVEKVSDVVKEGDVVKVKVLDIDGRGKVKLSMKEAETE"
BASE COUNT          990 a          445 c          432 g          967 t
ORIGIN      
        1 aaatataatg tttgcaggtg ggggagaaga attacattgg acaatgtcaa ttttatttga
       61 tgccatgggt gcattatcat caaaatataa tgaaactcct catcttgctt ctcgtgccta
      121 tgatacaaat agagatggtt ttgtaatatc aggaggtggt gggacaattg ttttagaaga
      181 gttagaacat gcaaaagcca gaggagctaa aatttatgct gaaatcacgg gttatggtgc
      241 cacttctgat ggtcacgata tggtacaacc atcaggcgaa ggagctgtta gatgtatgaa
      301 tcaagctctt aaaacaatgg atggagagat tgattacatt aattcacacg gtactagtac
      361 accaattgga gatcttaaag aattggaagc tataaaaaaa gtttttccta ataaatcccc
      421 tcacataagt tcaactaaat ctttaacggg acattcatta ggtgcaacag gtgttcatga
      481 aactatctat tctttattaa tgttaaataa taattttttg tgtggttcag caaatataag
      541 tgatctagat gaaagtgcta aagagcataa tattctaata gaaactataa ataaaaaaga
      601 catccaaaat gttatttcta atagttttgg ttttggcgga acaaatgcaa cattaggttt
      661 ttcaaaaata aatgcttaaa aataaaaaag gtataatttt tggtattgct aatgatcact
      721 caattgcatg ggggatagcc aaaaaactct cagaaaaggg tgcagaattt ggcattacat
      781 atcaaaatga aatattgcta aaaagagtta aacccttagc tgataaagtt aatagcagct
      841 tacttattga atgtgatttt tcaaaaacag aatctattaa aagttgtttt gaagaaataa
      901 aagcaaaatt tcaaaatatt gattttgtta ttcatgcagc ggcattttca gataagaatg
      961 aattgaatgg aaaatatata aacacatcaa aagataactt tataaaaact ctaaatattt
     1021 catgtttctc ttttacagaa atatctaagt atgcttctga aataatgaat gatggtggaa
     1081 gtttattaac tttaagtttt tatggatcta acaaagtgat gccaaactac aacgttatgg
     1141 gtgttgcaaa agcagcatta gaaactagcg ttaaatattt aagtgttgat ttaggtaata
     1201 aaaatataag agtaaatgct atttctgcag gacctatgcg tacactagcg ggggctgcga
     1261 tcgcaaatgc aagggatgtt tacaattata ctcaagcaaa ctctgcttta atacgaaatg
     1321 taaaattaga agaaattggt aatgctgctc tttattttgt tagtgattta tcaaatgcag
     1381 ttacaggtga ggtacattat gtagattgtg gttttaatat cttaggaatg ccaaaatcta
     1441 atacttaaga aatcttaaat tccttaggat catcaataaa aatacttttc actcccatag
     1501 accacaatgc attagcttcg cttggtttta aatttttttc tgaataaata gtagtaatca
     1561 aattatgtct tttcatttca ttcataatat ctgaattgat aatattttta ttaaaaccac
     1621 aacaagaaaa attatatttt tcacaaatat ctaaagtatt ttttaaagaa atatctttac
     1681 taaatccatc tattaaaagg ccataatatg aatttggtag aaattttttc attaaaatta
     1741 ccgaaggcca atcaaaactt gaaaaataat attgattggt aaaattaaag ttacttaaaa
     1801 gtttagcaat agcttcaaca ttattttttt caaaccccaa attaggtttt aattcaatgt
     1861 ttagacatat ttcgtttttt ttacaaaata aaagaacttg ttttaaagtt ggaatacaaa
     1921 tatttgtaga ataattgtaa aaggaaaaac ccgcatctag tttttttaaa tcatcatagt
     1981 taaagtctgt tggcaatccc ttaccatcag tggttctttc caaagtatca tcgtgcaata
     2041 aaatagggac taaatccttg gaaattttta catctatctc aatccacttc agtccaagtt
     2101 tgtgagccaa ttcaatagaa ttcaatgtat tttcaggaga taaattttta acccccctgt
     2161 gacctattaa tttaggaggg ttaagcattg tttttttatt cagtttccgc ttctttcatg
     2221 gataatttta ccttgccacg accatcgata tctaaaactt ttactttaac aacatcgcct
     2281 tctttgacaa catcactaac tttttcaact cgctcatttt ttagttgact aatatgcaca
     2341 aggccatcgc gtgaacccat aaaattaaca aaggcaccaa aatccattaa tttaacaact
     2401 ttaccttcgt agatttttcc aatctcaggc tcttcaacga tactattaat ccaatcaata
     2461 gcagcttgtc cggattccct atcaacagca gcaatactaa ctaaccctac atcattgatt
     2521 tcaatttttg cacctgtttt ttctgtaatt tcacgaataa cagctccacc ttttccaata
     2581 acttcacgaa tcttatcttt gtgaacttga attgtagtta tggtaggtgc gtagtctgaa
     2641 atactatctc tagatttatc aattgctttt gccatttcac ctaagatatg cagtctgcct
     2701 tctttcgctt gcacaagtgc ttccttcata atatccgaag taatgcttgt aattttaata
     2761 tccatttgaa gggatgtaat gccatcttta gtaccagcta ctttaaaatc catatcacct
     2821 aaatgatctt cgtc
//
LOCUS       DTVV01000312            1889 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1928540, whole genome shotgun sequence.
ACCESSION   DTVV01000312 DTVV01000000
VERSION     DTVV01000312.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1928540"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(16..831)
                     /locus_tag="EYO86_03725"
     CDS             complement(16..831)
                     /locus_tag="EYO86_03725"
                     /inference="COORDINATES: protein motif:HMM:PF13578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC42173.1"
                     /translation="MNKLSKYLSLIKFSFFQPKHGVELFQTYRQTNEDSKQTILPHEH
                     SAMEIEKCLKSLFPELDTKKDNLLDQTKKLGSDLQSFLEKIKKFEFPSKQQPYPIDYS
                     ISESSRLFLYALCKIIKPKTIVETGVAYGLSTSYILQALYENKTGTLYSIDSEFKPWE
                     SELMIGSAIPDELKTHWKFVKGSSTEKLNGVLESVGKIDIFLHDSMHTYKNMIFEFES
                     AWPHIKNNGFLLSDDILENNSFLEFYTRKNLKPILLSLLDQKHLFGILKKSEF"
     gene            complement(884..>1889)
                     /locus_tag="EYO86_03730"
     CDS             complement(884..>1889)
                     /locus_tag="EYO86_03730"
                     /inference="COORDINATES: protein motif:HMM:PF07995.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42174.1"
                     /translation="VYVGGDLASGLDGTVYAVTGENYKTGLLQNHLKNESYQHYSDKA
                     SFDEKDRRTILHSLTYALSCVKISFYHYTTNPVGWQSEQPDLSNNPLEFNLFNILGNL
                     DSCARQFYYENFSDGHWKDTSSIIQIEPKGEYAAIGIRNSFGLAVDPKTGYLWDTENG
                     PDTYDEINLVETKFNSGWAKIQGPSNGRLLPQLPNYEKYEYSEPEFSWELPIGVTAIE
                     FPNSKIFKKYENFVFVADVNNGIIYKFKLDDTRTKFVFESPHLQDNVLNIIENSENSV
                     HVDDTGCVISGYPCSGIEPMDEILFAKNLGVVTDMKFGPDGALYVISLMEGKIYRIAN
                     "
BASE COUNT          606 a          321 c          275 g          687 t
ORIGIN      
        1 aactataatt aaaaattaga attcagattt ctttaaaatt ccgaatagat gtttttgatc
       61 taacaatgat aataaaatag gttttagatt tttacgtgta taaaattcca agaatgagtt
      121 attttctaaa atatcatctg atagtaaaaa accattattt tttatgtgtg gccacgcact
      181 ttcaaactca aaaatcatat tcttgtacgt atgcatgcta tcatgtaaga aaatatctat
      241 ttttccgaca gattctaata caccatttag tttttcagtg cttgatccct ttacaaattt
      301 ccaatgagtt tttaactcat ctggaatagc tgagccaatc atcaattcag attcccatgg
      361 tttaaattct gaatcaattg agtacagtgt cccagttttg ttttcatata atgcctgtag
      421 aatatatgat gtactcaatc catacgcaac gccagtttca actatggttt tcggtttaat
      481 tattttgcac aacgcatata gaaaaagtct agaactttct gatatagaat aatcgatagg
      541 gtatggctgt tgtttagatg gaaactcaaa ttttttaatt ttttcaagaa atgattgtag
      601 atccgatcct agttttttag tttgatctaa aagattatcc ttttttgtat ctaactcggg
      661 aaaaagagat tttaggcatt tttcaatttc catagcagag tgttcatgtg gcagaatagt
      721 ttgtttcgaa tcttcatttg tttgcctata tgtttgaaat aattctacac catgttttgg
      781 ttgaaaaaat gaaaatttta tcaacgacag atattttgac agtttattca tattttacaa
      841 gaaaatttct tcaattagtg tttttttaat tatatgaagg agtttaattg gcaattctat
      901 agattttccc ttccattaac gaaataacat ataatgcgcc atcagggcca aatttcatat
      961 cagttaccac tcctaaattt ttagcaaata atatttcatc cataggttca attcctgaac
     1021 aaggataacc ggagatgaca caccctgtat catcaacatg gacagaattc tcagaatttt
     1081 ctataatgtt aagaacatta tcttgtaaat gcggtgattc aaaaacgaat ttcgttcttg
     1141 tatcatcaag tttgaattta tagataattc cgttattcac atctgctaca aatacaaaat
     1201 tttcatattt tttaaaaatt tttgagtttg gaaattctat tgcagttact cctataggca
     1261 gttcccagct aaattcaggt tcactatact catatttttc gtaatttggc aattgtggaa
     1321 gtaatctacc atttgacggt ccctggattt tcgcccaacc gctattgaac tttgtctcta
     1381 ctagatttat ctcatcataa gtatcaggac cattctcagt atcccacaaa tatcctgttt
     1441 ttggatcaac agctagccca aaactgtttc gaattcctat cgcagcatat tcgccttttg
     1501 gttctatctg aataatacta ctagtatcct tccaatgacc atcagaaaaa ttctcgtaat
     1561 agaactgtct tgcacaagaa tcaagatttc caagaatatt gaataagtta aattccaaag
     1621 ggttgtttga taaatcaggt tgttcagatt gccaaccaac aggatttgtt gtataatgat
     1681 aaaatgatat ttttacacat gataaagcat atgttaaact atgtaaaatt gtacgacgat
     1741 ctttctcgtc aaatgaagct ttatcgctat agtgctgata gctttcattt ttgagatggt
     1801 tttgtaatag acctgtttta taattttctc cagtaacagc atagaccgta ccatccaaac
     1861 cacttgcaag gtcaccgccc acatagacg
//
LOCUS       DTVV01000313            1228 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1931682, whole genome shotgun sequence.
ACCESSION   DTVV01000313 DTVV01000000
VERSION     DTVV01000313.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1931682"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="EYO86_03735"
     CDS             <1..1151
                     /locus_tag="EYO86_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HIC42175.1"
                     /translation="VAQLSATIPDLLPPEYAQELTHLQSNAPPMGWLFVKRRMVTELG
                     LHWQKNFNKFDKEATRAASLGQVHKAKLLSGKEVACKLQYPDMESAVTADLSQLKLVF
                     SIYQAYNKAIKTDEVYKEITQRLTEELDYERERKLTQIFGKIFSKSSFVHIPGTIDKL
                     STKRLLTMSWLEGKSILDFKNAKKEVRNTIAKHMFYTWYKPFYEYGIIHGDPHLGNYT
                     IQPNYTINLFDFGCMRIFKGKFIQGVIDLYYALQNNDDEKAVHAYSQWGFADMTKKKL
                     EVLNKWAGFIYSPLMEDKIQKIQQSDSGIYGAKVAAEVHKELKKLGGVMPPKEFVFMD
                     RAAVGLGSVFMHLKAEVNWYKIFHSLIENFETKKMNARQQKILKLVKL"
BASE COUNT          458 a          179 c          224 g          367 t
ORIGIN      
        1 aagttgccca attatccgca acaattcctg atttattgcc cccagagtac gctcaagaac
       61 taacccatct tcaatcaaat gctcctccaa tgggatggtt atttgttaaa agaagaatgg
      121 tgacagaatt aggtcttcat tggcaaaaaa attttaataa atttgacaaa gaagcaacaa
      181 gagctgcctc tttagggcag gtgcataagg ccaagctttt gagtggaaaa gaggttgctt
      241 gcaaattaca atatcctgat atggagtcag cggttacagc tgatttatct cagttgaaat
      301 tagttttttc tatttaccaa gcttataaca aagccataaa aactgatgaa gtgtataaag
      361 agattacaca aagattaaca gaagagctcg actacgaacg tgaaagaaaa cttactcaaa
      421 tatttggcaa aatattttca aagtcatctt ttgttcatat tcctggaaca attgataaat
      481 tatcaacaaa aagattatta acaatgagtt ggttagaggg gaagtccata ttagatttta
      541 aaaatgcaaa gaaagaagtt cgcaatacaa ttgcaaaaca tatgttttat acttggtata
      601 agccatttta tgaatatgga attattcatg gcgatccgca tttaggaaat tatactatcc
      661 aaccaaacta cactatcaat ctttttgatt ttgggtgtat gagaatattt aaaggaaagt
      721 ttattcaagg ggtcatagat ctttattacg ctctccagaa caatgatgat gaaaaagcag
      781 tccacgcata ttcacaatgg gggtttgcag atatgactaa aaaaaaactt gaagtactca
      841 ataaatgggc aggatttatt tactctcctt taatggaaga taaaattcaa aaaatacaac
      901 aaagcgacag tggtatttat ggtgcaaaag ttgcagctga ggttcataag gagctaaaaa
      961 aattaggagg tgtcatgcca cctaaggaat ttgtatttat ggatagagca gctgttggac
     1021 tggggtctgt tttcatgcat ttaaaagctg aagttaattg gtataaaatt tttcatagtt
     1081 taatagaaaa ttttgaaaca aaaaaaatga atgctagaca acaaaaaatt ttgaaattag
     1141 taaagcttta aaatataaat ctatatgcaa atttctatta ttaaagaagt aaatccacgt
     1201 gagcaacgtg tggcagcttc acctgaat
//
LOCUS       DTVV01000314            1460 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1934406, whole genome shotgun sequence.
ACCESSION   DTVV01000314 DTVV01000000
VERSION     DTVV01000314.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1934406"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(219..614)
                     /gene="mntR"
                     /locus_tag="EYO86_03740"
     CDS             complement(219..614)
                     /gene="mntR"
                     /locus_tag="EYO86_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HIC42176.1"
                     /translation="MSFKKVRDAHQTENTEDYLELIADLLNQHGEARIVDIASKLDIT
                     QATVNKTIKRLQIQGYIQKEPYRSIFLTVKGQQVASQSKKRHKIVFNFLSNMGLDKKT
                     AESDSEGIEHHVSDRTLKKMNKFNKTKKF"
     gene            698..>1460
                     /locus_tag="EYO86_03745"
     CDS             698..>1460
                     /locus_tag="EYO86_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008282829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIC42177.1"
                     /translation="METQTIILYGSLASLGAGMMTTVGAIPCLFIKKISPKFEDTTLG
                     FAAGVMLGASFFSLIVPGLDYADELWQSKYLSVLAVLGGLLAGVLGIWSIEKYSPHEH
                     FVIKEHYDGGHKEKILKIWLFIIAITIHNMPEGLAVGVGFGGGDLGKAVALAIGIGIQ
                     NAPEGLAVAFALLTIGYSRMNAFLIAGATGLFEPVMGFIGVSIVTVFLPILPIALGFA
                     AGAMLFVISHEIIPETHRRGHENWATGGFTFGLLTM"
BASE COUNT          385 a          237 c          305 g          533 t
ORIGIN      
        1 acatcaacat tttgatcaac aattttggca atgctacttg tatatgctgt taagcaagaa
       61 ataggccgta tattttttag ccgctgtatt gtttttattg agttgcgctt cataatgtat
      121 attaccacta acaaagaaga atgctgagat ataaattaag ttttttctgt ccctctgtat
      181 tattcgtcca cataattcgt tttggtatta atgctaaatt aaaatttttt tgttttatta
      241 aatttgttca ttttttttag ggttctgtca cttacgtgat gttctattcc ctcagaatct
      301 gattcagccg tttttttatc taaacccatg ttggataaaa aattaaaaac tatcttatgt
      361 cttttctttg actggctagc aacctgttgt cctttgacag ttaggaatat tgagcggtaa
      421 ggttcttttt ggatatagcc ttgtatctgt aaccgcttaa tggttttatt cactgttgct
      481 tgagtgatat ctaatttcga ggcaatatcc acgattctgg cttcgccgtg ttgattcaat
      541 agatcagcta tcagttcaag atagtcttcg gtgttttccg tttggtgggc atctcgtacc
      601 tttttaaaag acatattatg gtattattat aaaaatataa acatttcatc attaatatat
      661 atagctatgg ctatattttt tagctatacg cacggaaatg gaaacacaaa ccatcatttt
      721 atatggatct ttagcaagtt tgggggctgg catgatgact acggtagggg ctataccttg
      781 cttattcatt aaaaaaatat caccaaaatt tgaagataca acattgggat ttgcagcggg
      841 cgtaatgctg ggcgcttcat ttttttcact tatcgttcca ggattagatt acgccgatga
      901 gctttggcag agcaaatatc tttctgtgct ggctgtgctt ggaggcttat tagctggtgt
      961 cttggggata tggtcaattg aaaaatattc gcctcatgag cattttgtta ttaaagagca
     1021 ttatgatggc ggacacaaag agaagattct aaaaatatgg cttttcatca ttgccataac
     1081 cattcacaat atgccggaag ggcttgctgt aggtgtcggt tttggtggcg gtgacttagg
     1141 taaggcggtt gctttagcga ttggcattgg tatacaaaat gcgccagagg gattggctgt
     1201 agcatttgct ttgcttacca ttggatattc aagaatgaat gcttttttaa tagctggggc
     1261 gacaggtttg tttgagcctg taatgggatt tataggtgtg tcaattgtca cagtattcct
     1321 tcccatactt cccatagcgt tgggttttgc tgcaggtgcc atgctttttg ttatatcaca
     1381 tgaaattata cccgaaacac atagacgggg acatgaaaat tgggctacag ggggttttac
     1441 ttttggtctt ttaacaatgt
//
LOCUS       DTVV01000315            1475 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1934931, whole genome shotgun sequence.
ACCESSION   DTVV01000315 DTVV01000000
VERSION     DTVV01000315.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1934931"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /gene="hppA"
                     /locus_tag="EYO86_03750"
     CDS             <1..783
                     /gene="hppA"
                     /locus_tag="EYO86_03750"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_426905.1"
                     /note="pyrophosphate-energized proton pump;
                     pyrophosphate-energized inorganic pyrophosphatase;
                     H+-PPase; can cleave pyrophosphate to two phosphates; can
                     generate a proton motive force and drive pyrophosphate
                     synthesis when PMF is sufficient; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIC42178.1"
                     /translation="AEMSNLPKSVRKTTDALDAVGNTTKAITKGYAISSAGLGALVLF
                     AAYTEDIKFFSNIKGSALENISVNFDLSNPFVMVGLLIGGMLPYLFGSMSMQAVGRAG
                     GAVVIEVRRQFKKIPGIMKRKRKPDYSKLVDLLTKAAIKEMIIPSLLPVLSPIILYFI
                     ILLIGGIEAALAAVGAMLLGVIVTGFFVAISMTAGGGAWDNAKKYIEDGKFGGKGSEA
                     HKAAVTGDTVGDPYKDTAGPAVNPMIKITNIVALLLLAVIAH"
     gene            complement(780..1253)
                     /gene="bamE"
                     /locus_tag="EYO86_03755"
     CDS             complement(780..1253)
                     /gene="bamE"
                     /locus_tag="EYO86_03755"
                     /inference="COORDINATES: protein motif:HMM:PF04355.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIC42179.1"
                     /translation="MLYIFRHMKETVYLLLILLFTISCQRNEIVKTHGIAYLEKREKL
                     IFVNKSNKNDTINIFGQPSTKGMTNDNLWIYIERTMTKGKMFKLGRNYLTKNNVLVLE
                     FDKYGILKKKELSSIEDMKKLTFAKNITENEIRKENFVYSFLSSVRQKMQTQRKD"
BASE COUNT          496 a          207 c          233 g          539 t
ORIGIN      
        1 gctgaaatgt cgaatttacc taaaagtgtt agaaaaacaa cagatgcctt agatgctgtt
       61 ggcaatacga caaaagctat taccaaagga tatgctataa gttccgctgg attaggtgca
      121 ttggttttat ttgctgctta tacagaagat attaaatttt tttctaacat taaaggatct
      181 gctctcgaaa atatttcagt aaattttgat ctttccaatc cattcgttat ggttggtcta
      241 ttaatcggag ggatgttgcc atatttattt ggatcaatgt caatgcaagc tgttggaagg
      301 gcaggaggtg ctgtcgtaat tgaagtcaga aggcagttta aaaaaatacc aggaattatg
      361 aaacgcaaaa gaaaacctga ttacagtaag ttagtggact tattaaccaa agcagctatc
      421 aaagaaatga taataccatc attattacca gtattatcgc ctataatttt atactttatc
      481 atattattga taggtggaat tgaagcggcg ctagctgctg ttggagcaat gctgttagga
      541 gttattgtaa caggtttctt tgttgccatt tcaatgactg caggtggggg tgcctgggat
      601 aatgcaaaaa aatatattga agatggtaaa tttggtggaa aaggatctga ggcacataaa
      661 gctgctgtta ccggagatac cgtgggtgat ccatataaag atacggcagg tccggctgtt
      721 aatccaatga ttaagataac aaatatagta gctttattac tattagctgt aattgctcac
      781 taatcttttc tttgtgtttg cattttttgt ctaacgctag acaagaaact atatacaaag
      841 ttttcttttc gtatttcgtt ttcagtaata ttttttgcaa aagttagttt tttcatatct
      901 tctatagaag acaattcctt ttttttaagg atcccgtatt tgtcaaactc taatactaaa
      961 acgttatttt ttgtaagata atttctacct aacttaaaca tttttccttt agtcatagtt
     1021 ctttcaatat aaatccataa attgtcattt gtcattccct ttgtggatgg ctgtccaaaa
     1081 atattaattg tgtcattttt attcgatttg ttaacaaata ttaacttctc ccttttttct
     1141 aaatatgcta ttccatgtgt ttttactatt tcattgcgtt ggcaactaat ggtaaaaagt
     1201 aaaattaata acaaatatac agtttctttc atatgtcgaa atatataaag caaatcaata
     1261 cccaagaaga gatattatat aataatattc ttttattatc acgaaataaa ttattttaca
     1321 ctaaatttga tttgattgat acatttcaaa atcgaattca cttaattttt atacatattt
     1381 catttatatt tattaaaata aaacaaaata acaaaagtca aatatataaa attttttatc
     1441 aaaaagtatt cgatttaaca tttaataaaa ttgaa
//
LOCUS       DTVV01000316            1086 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1936532, whole genome shotgun sequence.
ACCESSION   DTVV01000316 DTVV01000000
VERSION     DTVV01000316.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1936532"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..1000)
                     /locus_tag="EYO86_03760"
                     /pseudo
     CDS             complement(1..1000)
                     /locus_tag="EYO86_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          334 a          206 c          140 g          406 t
ORIGIN      
        1 ccttgccatt agatactatg cggtaaggag tttctataaa tccatattgg ttaattctag
       61 aataagaagc aaggctatta atcaagccga tatttgctcc ctcaggtgtc tcaattggac
      121 atattctacc ataatgagtt tgatgaacat cacgaacttc aaaacctgct ctttctctat
      181 ttaatcctcc aggcccaaga gcggaaactc ttcttttatg agtaatttca ctaagaggat
      241 ttgtctgatc cataaattgg ctcaattggc ttgtcccaaa aaattcttta attgatgctg
      301 acacaggttt tgcgtttata atatcttggg gcataatatt atcaacctca agtgaactta
      361 agcgttcttt aattgctcta tccattttta ataaaccaac cctgtattgg ttttcaagaa
      421 gttctccaac agaacgaatt cttctatttc caagatgatc aatatcatct acctcacctt
      481 ttccatctat aagattgtgc atgtgcttaa taacatccaa aatatcactc ttatcaagaa
      541 ctcttttttc aattggcgtg tcttttttaa atttagaaat tatctttaat ctgccaactg
      601 cagacaaatc atatctatct gcattaaaaa acaaattgtt aaaaagcatt tcagctgtct
      661 caattgttgg aggctcgcct ggtctaagaa ttttaaatat ctcaaataac gcctcctctc
      721 ttgatcgaac cttgtctaat tgcagagtac ttctgatata tgaaccaatt tcaatattat
      781 cggttcttag aatatcaatt ttggtaatat ttttttcatt aataaaatcg ataaaatctt
      841 gatcaatttc aaaacctgct tcgtaaaaaa ttttacctgt tttttcatct ataatatcac
      901 ttgacaaata atgtccaagt aatgaatttt cttcaatagt taaagaattt atcttctttt
      961 ttaataattc attaattgat ttttggttaa gttttgatcc ttttactatt ttagctctat
     1021 tttgacaact tgctctgttt tagtccagcc tctagaaaca tagagaaagc cctttcacct
     1081 atttca
//
LOCUS       DTVV01000317            3950 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1940286, whole genome shotgun sequence.
ACCESSION   DTVV01000317 DTVV01000000
VERSION     DTVV01000317.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1940286"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="EYO86_03765"
     CDS             complement(<1..1089)
                     /locus_tag="EYO86_03765"
                     /inference="COORDINATES: protein motif:HMM:PF00205.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIC42180.1"
                     /translation="MINMRVADFVANFIYEELNVQHVFTVTGAGIMHLTDGLISHKKL
                     KTICPHHEQTASMALEAYSRATENFGVGIFTTGPGCTNTITGLAGAWQDSVPCLFISG
                     QVKKSEASANSGIKNLRQFGVQELDIIPIIKSITKYAVTINRPEKIRYELEKAVHIAK
                     TGRPGPVWIEIPMDVQSAKINDELEQYIPEKNTKPAINKKDIEHLSTLLKNSQRPVII
                     AGQGIRIAGAIECFNEFVEKYGIPVVTPYLGIDVMKHNDKHYIGKSGVKGDRPANFAM
                     QNSDLVISIGSSLHVTVIGYNYLQFAREAKKIIIDIDDTSHQKKTIKKDLFIQADAKD
                     LLEKLLENLKNIKLNNYSNWLEKCNLWKK"
     gene            complement(1086..2261)
                     /locus_tag="EYO86_03770"
     CDS             complement(1086..2261)
                     /locus_tag="EYO86_03770"
                     /inference="COORDINATES: protein motif:HMM:PF00155.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIC42181.1"
                     /translation="MKKLSELSKRIDGQPMFKLLDKVKKLEKEGKNIIHFEIGDPDFG
                     TPVNIVDAGINAIENGFTHYTSSYGLTEFREKICDATLKSRHFKPDIEQILVTPGANI
                     AIFYAIICLVDQGKEVIVPDPGFPSYYSTIKMCNVTPVRVPLKEENEFRMNPDDIEKN
                     ITENTRLIIINSPQNPTGSVMTEDQIKHTYEIAEKHDVYLYSDEIYARMIYDNTKFNS
                     PSIYDKCKDRTIISNGFSKAFAMTGWRLGAIIGPSVVIEKMKLLLETTSSCVSPFIQK
                     AGIEAISGDQSRTINMYKEYNERRELIVDGLNSITSISCVKPSGAFYVFPNIKNTGMT
                     SIEFANYILDDANVAVLPGTNFGEYGEGYVRLCYATTKEKINEAIKRIDLSMRKLNL"
     gene            2439..3347
                     /locus_tag="EYO86_03775"
     CDS             2439..3347
                     /locus_tag="EYO86_03775"
                     /inference="COORDINATES: protein motif:HMM:PF01370.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC42182.1"
                     /translation="MNKIAILGANGHIGKNLISYFSTSNHNLFLFSRKKKLFSKFIKN
                     FHFTNSPKICDYKKFEKFHYDLIINCVGSSDPAKIAENARIQNITEYYDNKIINYLIE
                     NQKSMYIFFSSGIVYDGEFKNPVKSTTKLSMNINKTKKSDFYKISKINAEIKHRLLSD
                     LNIVDIRIFSFFSKFLDLNSSFLMADIVNSVKNKTVFVTNSQNIKRDFVHPKDLFQLI
                     VLCMKQKSMNSAIDLYSKKPISKFEIIKYFQDKYGLTYKIKKILKNKTKTNYYTTKKF
                     NELGYKPKFSSMDSIMDESKFFFDYD"
     gene            3402..>3950
                     /locus_tag="EYO86_03780"
     CDS             3402..>3950
                     /locus_tag="EYO86_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIC42183.1"
                     /translation="MKKVLITGVTGQDGAYLSKLLLSKGYKVFGTFRRVSTPNFWRLQ
                     NLGIFNKINLIPADLLDMGSLLEALTVSEPDEVYNLAAASYVATSFEEAVGNAEITGL
                     AVTKFLEAIRHQDSNIKFYQASSSEMYGNNDFELQDEKTPFLPSSPYAAAKLYAHSIS
                     NIYRQSYDIFACSGILFNHESPL"
BASE COUNT         1314 a          660 c          521 g         1455 t
ORIGIN      
        1 cttcttccat aaattacatt tttcaagcca atttgaataa ttatttaatt ttatattttt
       61 taaattttca agtaattttt ctaagagatc ttttgcatca gcttgtataa acaaatcttt
      121 cttaatcgtc ttcttctggt gtgatgtgtc atctatatct atgataattt tttttgcctc
      181 cctagcaaat tgtaaataat tatacccgat tactgtaaca tgtaaactag aaccaattga
      241 aatcactaaa tcagaatttt gcattgcaaa atttgctggt ctatcacctt taacaccaga
      301 tttacctatg tagtgtttgt cattatgttt cattacatct attcctaaat atggtgtaac
      361 tacaggaatt ccatatttct caacaaattc attaaaacat tcaatggctc ctgcaatcct
      421 aattccttga ccagcaatga ttactggtct ttgtgaattt ttgagaagtg ttgataaatg
      481 ttcaatatct tttttattta tagccggttt tgtatttttt tctggaatat attgttctaa
      541 ttcatcatta atttttgcac tttgtacatc cattggaatt tctatccaaa ctggaccagg
      601 ccttcctgtt tttgctatat gaactgcttt ttccaattca taacgaattt tttctggtct
      661 gttaatggtt acagcatact tagtaattga ttttataatt ggtattatat caagttcttg
      721 aactccaaac tgcctcaaat ttttaattcc cgaatttgct gatgcttcag atttttttac
      781 ttgacctgag ataaataaac atgggactga atcttgccat gctccagcta aacctgtaat
      841 tgtattagtg catccaggtc cagttgtaaa aattcctact ccaaaatttt cggttgccct
      901 agaataagct tctaaagcca ttgaagctgt ttgttcgtga tgaggacata ttgtctttag
      961 ttttttatga gatatgagac catcagttaa gtgcatgatt cctgcacctg taactgtgaa
     1021 gacatgctga acattcaatt cttcataaat aaaattcgcc acaaaatctg caacacgcat
     1081 gttaatcaca aatttagttt tctcattgat aagtcaattc ttttgatcgc ttcattaatt
     1141 ttttcttttg tagttgcata acataatcta acatatccct ctccatattc tccaaaattt
     1201 gttcccggca gaacagcaac gtttgcatcg tctagtatgt aattagcaaa ttcaatacta
     1261 gtcatacccg tattttttat gttaggaaaa acataaaatg cgccacttgg ttttacacag
     1321 ctaatactcg tgatcgagtt caagccatct acaattaatt ctcgcctttc attgtattct
     1381 ttatacatgt taatcgtacg tgactgatca cctgatatgg cctcaatgcc tgccttttga
     1441 ataaatggtg atacacatga agaagttgtc tctaataata atttcatttt ttcaataaca
     1501 acagatggac ctattattgc acctaacctc catcccgtca ttgcaaaagc cttactgaaa
     1561 ccattagaga ttattgttct atctttacat ttatcataaa ttgatggaga attgaatttt
     1621 gtattatcat aaatcattct tgcatagatt tcatcactgt acaaatacac gtcatgtttt
     1681 tctgcaattt cataagtgtg tttgatttga tcctcagtca tcacagagcc agttgggttt
     1741 tgcggtgaat ttattataat cagtcttgta ttttcagtaa tattcttttc aatatcatct
     1801 gggttcattc taaattcatt ttcttctttt aatggaactc taacaggagt tacattgcac
     1861 atcttaattg tagaataata acttggaaat cctggatcag gaacaataac ttctttgccc
     1921 tgatcaacta aacatataat tgcataaaaa attgcaatat ttgcccctgg ggtaactaat
     1981 atctgctcaa tatctggttt aaaatgtcta gatttcagcg ttgcatcaca gattttttct
     2041 ctaaactcag ttaatccata cgaacttgta taatgcgtaa atccattttc tattgcatta
     2101 attccagcat caacaatatt tactggagtt ccaaaatctg gatcaccaat ttcaaaatga
     2161 attatatttt taccctcctt ttcaagtttt tttaccttat caagaagttt aaacatgggc
     2221 tgaccatcga ttctttttga taattctgat aattttttca tattaatttt ttccaattat
     2281 acctaaaaat cgttgttatt aaaacatcaa ttatttacga gtgtgttata ctacaataaa
     2341 attaaatttt ttctaataaa tgaaaaaaat aatttggtat attacaatgt taatttatta
     2401 ttagtattac atttattgtt gtaaaatttt aatttgtcat gaacaaaatt gcaattctag
     2461 gtgctaatgg acatataggt aaaaatctta tctcgtattt ttcaacatca aatcataatc
     2521 tttttctttt ttctagaaaa aagaaattat tttcaaaatt cattaaaaat ttccatttta
     2581 ctaattctcc taaaatctgt gattataaaa aatttgaaaa atttcattac gatctaatca
     2641 ttaactgtgt tggttcaagt gaccctgcta aaatcgctga aaatgcccgt atacaaaata
     2701 ttactgaata ttacgataat aaaattatca attatctaat cgaaaaccaa aaatcgatgt
     2761 atattttctt tagtagcgga atcgtttatg atggtgaatt taaaaaccca gtcaagtcta
     2821 cgacaaaatt atcaatgaat attaataaaa ctaaaaaatc tgatttctat aaaatttcca
     2881 aaatcaatgc tgaaataaaa catcgtttat tatcagattt gaatattgta gacattcgaa
     2941 ttttttcatt ttttagtaaa tttcttgatc ttaattcttc atttcttatg gcggatattg
     3001 tgaattctgt aaaaaataaa acagtctttg ttactaattc acaaaatatt aagcgtgatt
     3061 ttgtccatcc caaagattta ttccagttaa tagtactttg tatgaaacaa aaatcgatga
     3121 acagtgcaat tgatctatac agtaaaaaac caatttcaaa atttgaaatc ataaaatact
     3181 tccaagataa atatgggcta acatataaaa ttaaaaaaat tctaaaaaat aaaaccaaaa
     3241 caaattatta tactacaaaa aaatttaatg agcttggata caagccaaaa ttctcatcta
     3301 tggattcaat tatggacgaa tctaaattct tctttgatta cgattaactt gcaaatcatt
     3361 tattcctaaa caatattaat agtaaaaata cctgaaaaca aatgaaaaaa gtactgatta
     3421 ctggtgtcac tggacaagat ggtgcttatc tttcaaaatt attattatca aagggctaca
     3481 aggtttttgg tacctttagg agagtttcca ccccaaattt ttggagattg caaaaccttg
     3541 gaatttttaa taaaatcaat ctaatcccgg cagatctact tgatatgggc tcgctattgg
     3601 aagcactgac ggtatcagag ccagatgaag tatacaatct tgcagctgct agctatgttg
     3661 caacatcatt tgaagaggct gttggcaatg cagagattac tggtcttgca gtaacaaaat
     3721 ttttggaagc gattagacac caagattcga atataaaatt ttaccaggca tcatcaagtg
     3781 aaatgtatgg gaataatgat tttgaattac aggatgaaaa aactccattt ttaccatcaa
     3841 gcccatatgc agctgcaaaa ttgtatgcac actcgatatc aaacatctat agacaatcct
     3901 atgatatttt tgcgtgctct ggaatattat ttaatcatga atcgccttta
//
LOCUS       DTVV01000318            1984 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1943824, whole genome shotgun sequence.
ACCESSION   DTVV01000318 DTVV01000000
VERSION     DTVV01000318.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1943824"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /gene="rpsM"
                     /locus_tag="EYO86_03785"
     CDS             <1..208
                     /gene="rpsM"
                     /locus_tag="EYO86_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIC42184.1"
                     /translation="IIETQHSVEGDLRRTISMNIKRLTDLGCYRGLRHRKKLPVRGQR
                     THTNARTRKGKAVAIAGKKKVTKG"
     gene            212..598
                     /gene="rpsK"
                     /locus_tag="EYO86_03790"
     CDS             212..598
                     /gene="rpsK"
                     /locus_tag="EYO86_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIC42185.1"
                     /translation="MAEKKKTKTKIKKKVTSGVAHVLSSFNNTIITITDENGNTLAWS
                     SAGQKGFKGSRKSTPFAAQVAAEDVGTKAKEYGLKSLRIEVSGPGSGRESALRSLQTI
                     GYIITSIKDVTPIPHNGCRPRKRRRV"
     gene            623..1645
                     /locus_tag="EYO86_03795"
     CDS             623..1645
                     /locus_tag="EYO86_03795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIC42186.1"
                     /translation="MLQKNWMDLIKPNKMDVNVQENDGRFGILTAEPLERGFGLTLGN
                     SLRRILLSSLQGAAITSIKIKGIVHEFSTIPGVKEDLTDILLNLKAVGIKVHSPGLRK
                     MYIKTKGSGEVRAGNFETDSETEIMNPDQIIMTLDSNADVELEANVDTGKGYLSAEVA
                     EDDNKIIGEIKLDAMFSPIIRASYKVENSRVGQVTDFDKLILEVETNGAVSPDDAIAL
                     ASRILQDQLQPFINFDEPEIEQDVSSHDKLSFNPNLLKKVEELELSVRSANCLKNDNI
                     IYIGDLVQKSESEMLRTPNFGRKSLNEIKEVLQQMELGLGISVPDWPPENIEELAKKF
                     EDPFNK"
     gene            1651..>1984
                     /locus_tag="EYO86_03800"
     CDS             1651..>1984
                     /locus_tag="EYO86_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIC42187.1"
                     /translation="MKHNITQKKLNRTTSHRKALLMNLSNALIKHEQITTTLPKAKAL
                     RPFVEKIITLGKNGDLTSRRKAISILQDDKNIKKIFDIIADRYKERSGGYTRIVKIGN
                     RFGDNAPTA"
BASE COUNT          776 a          282 c          375 g          551 t
ORIGIN      
        1 aataattgaa acacaacatt ctgttgaagg cgacctgcgt agaacaattt ccatgaacat
       61 caagagatta actgatttag gttgttatag gggtttgcgt catagaaaaa aacttccagt
      121 tcgaggtcaa agaacacata caaatgcaag aactagaaaa ggtaaggcag ttgcgattgc
      181 tggaaagaaa aaagtaacca aaggttaata catggctgaa aagaaaaaaa ctaaaactaa
      241 aataaaaaaa aaggtaacaa gtggggttgc tcatgttctt tcatcgttta ataatacaat
      301 tattacaatt accgatgaaa atggaaatac acttgcttgg tcatctgcag gccaaaaagg
      361 ttttaaaggc tcaagaaaat caactccttt tgcagctcaa gttgcagctg aggatgttgg
      421 cacaaaggca aaagagtatg gactaaagtc attgagaatt gaggtatcgg gacctggatc
      481 tggtagagaa tctgcactta gatctcttca aactattgga tatatcataa cttcaataaa
      541 agatgtgact ccaattcctc ataacggatg tagaccaaga aaaagaagac gagtttaaat
      601 tatataatac aaggagtatc gagtgttaca aaaaaattgg atggatttaa ttaaaccaaa
      661 taaaatggat gttaatgttc aggaaaatga cgggcgtttt ggaattttaa cagctgagcc
      721 actagaaaga ggttttgggc taacattagg aaattctcta agaagaattt tattatcatc
      781 tcttcaagga gcagcaatta catctataaa aattaaaggt atagtgcatg aattttcaac
      841 aatacctggt gtaaaagaag atttgactga catcctttta aatttgaaag cagtaggaat
      901 taaagttcat tctcctgggt taagaaaaat gtacattaaa actaaaggtt ctggtgaagt
      961 tagagccggt aattttgaga cagattcaga aacagaaata atgaatcccg atcaaattat
     1021 catgactctc gatagcaatg cagatgtaga gcttgaggct aatgttgata caggtaaagg
     1081 atacctttct gcagaagtag ctgaggacga taacaaaata atcggtgaaa ttaaattgga
     1141 tgcaatgttc agtcctatta tcagagcttc ttataaagtt gaaaatagtc gggttggtca
     1201 agtaacagat tttgataaat tgatacttga agttgaaaca aatggggcag tatctccgga
     1261 tgatgccatt gctttggcat caagaatatt acaagatcaa ttacagccat ttattaattt
     1321 tgatgaacct gaaatagagc aagatgtaag ctctcatgat aaattatcat ttaatcctaa
     1381 tttacttaaa aaagttgaag aattggaact ttcagtccgt tctgcaaatt gtttaaaaaa
     1441 tgataatata atttatattg gcgatcttgt gcagaaatca gagtctgaaa tgcttagaac
     1501 accaaatttt gggagaaaat cacttaatga aataaaagaa gtattgcaac aaatggaact
     1561 aggattgggt atttctgtac cagattggcc accagagaat attgaagaat tagcaaaaaa
     1621 atttgaggat ccatttaaca aataactgta atgaaacata atattacaca aaaaaagtta
     1681 aatagaacta cttcacatag aaaagcttta ttaatgaatt tatccaatgc cttgataaaa
     1741 catgagcaaa ttacaacaac tctccctaaa gcaaaagcac taagaccatt tgttgaaaaa
     1801 attattactc ttggcaaaaa cggagattta acatccagaa gaaaagctat ttctatcttg
     1861 caggatgata aaaatataaa aaaaatattt gatataattg ctgatcgtta taaagaaaga
     1921 tcgggaggat acacacgaat tgttaaaatt ggcaaccgct ttggtgacaa tgcgcctaca
     1981 gcaa
//
LOCUS       DTVV01000319            1332 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1946873, whole genome shotgun sequence.
ACCESSION   DTVV01000319 DTVV01000000
VERSION     DTVV01000319.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1946873"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="EYO86_03805"
     CDS             <1..366
                     /locus_tag="EYO86_03805"
                     /inference="COORDINATES: protein motif:HMM:PF01612.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIC42188.1"
                     /translation="NLQYFLKCEVKNIWDSKVASRLVRTYSQNHGLKDLVSEFCGGKK
                     LNKQYSSSDWNKNLDEMTDAMLQYAAGDVIYLHKIKDALHKMLVRENRLELFESCMKF
                     LPSRIKLDEKLFTEDIFAH"
     gene            370..>1332
                     /locus_tag="EYO86_03810"
     CDS             370..>1332
                     /locus_tag="EYO86_03810"
                     /inference="COORDINATES: protein motif:HMM:PF01380.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIC42189.1"
                     /translation="MNKDIKVAKQTINTEIQALKKLLASFNRSSQFSKAVNLISKIRG
                     KCLVVGVGKSYLCGLKTASTLSSLGTPSVAFSANDLQHGGLGAIQKNRDVLLVFSVSG
                     ESAELNSILQYANRNGIPVIGVSCKPSSMLLRYSTIKILLPKVIESGHSLAPTSSSLN
                     FLSWGDSLAISLMKRKKWTNKKFVEHHTGGSLATALIQVREIMAKGKEIPLISSNKMM
                     RAAITEMNKKKLGIVCVKDKNSIMLLTDGDIRRHSNNLYKKKIVKVATKNPTWIADTD
                     TALSAINTMNTKGITSLLVAKKRDIKKKIKRLVGVLHMHTALALG"
BASE COUNT          510 a          204 c          240 g          378 t
ORIGIN      
        1 aatttacaat attttctaaa gtgtgaggta aaaaatatct gggattccaa ggttgctagc
       61 cgattggtga gaacatacag ccaaaatcac ggattgaaag atttagtttc tgaattttgt
      121 ggaggaaaaa aacttaacaa acagtatagt tctagcgatt ggaataagaa tctagatgag
      181 atgacagatg ctatgctcca gtatgctgct ggagatgtaa tttatttaca taaaataaaa
      241 gatgcgttgc acaaaatgtt agtaagagaa aacagactag aactctttga aagttgtatg
      301 aaatttttac caagtcgcat caagttagat gaaaaattat ttaccgaaga tatttttgca
      361 cactgaaaaa tgaataaaga tataaaagtc gcaaagcaaa ccataaacac agaaatacaa
      421 gcgctcaaaa aattattggc gagttttaat cgttcttctc aattttcaaa agctgttaat
      481 ttaatttcta aaattagagg aaaatgtttg gtcgttggag taggtaaaag ttatttatgt
      541 gggctcaaaa ccgcctcaac tttatcaagt ttaggtacac caagtgtagc tttttcggca
      601 aacgatttgc aacacggtgg cttaggtgct attcaaaaaa atcgtgatgt actattggta
      661 tttagtgttt caggagaatc tgcagaatta aatagtattt tgcaatacgc taatagaaac
      721 ggcattccag ttattggtgt aagctgtaaa ccctcttcaa tgctacttcg ttattctaca
      781 atcaaaattt tactaccaaa agttattgaa tcaggccata gtctagcccc aacatcatca
      841 agtttaaact ttctttcttg gggggactca cttgctatat ctttaatgaa gagaaaaaaa
      901 tggacgaata aaaaatttgt agaacaccat actggaggat ctctagccac agctttaatt
      961 caagtaagag aaataatggc aaaaggaaaa gaaattccac ttatttcttc taacaaaatg
     1021 atgagggccg caataactga aatgaacaaa aaaaaactgg gaatagtttg tgtaaaagac
     1081 aaaaattcaa taatgctact aacagatgga gatattcgaa gacactccaa taatttatat
     1141 aaaaaaaaaa tcgtaaaagt tgcaactaaa aatccaacct ggattgcaga tacagatacc
     1201 gcactttctg caattaatac aatgaacacc aaaggtataa catcacttct agttgcaaaa
     1261 aaaagggata tcaaaaaaaa gattaaaaga ctggtgggcg tcctacatat gcatactgct
     1321 ttggcactgg ga
//
LOCUS       DTVV01000320            3027 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1949258, whole genome shotgun sequence.
ACCESSION   DTVV01000320 DTVV01000000
VERSION     DTVV01000320.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1949258"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="EYO86_03815"
     CDS             complement(<1..417)
                     /locus_tag="EYO86_03815"
                     /inference="COORDINATES: protein motif:HMM:PF07015.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIC42190.1"
                     /translation="MGKIITFANQKGGSGKTTLSANLAVLWANSNYKVAVIDADAQRS
                     LTNWLEARKKYYRDEPTGIDSYPFDVRKLEEDLKQIKKKYNFIIIDSPPSITFDTVQI
                     IKSSDKVFVPVQPSPLDLMATVPFLNLVRQERKKTTV"
     gene            493..1425
                     /locus_tag="EYO86_03820"
     CDS             493..1425
                     /locus_tag="EYO86_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HIC42191.1"
                     /translation="MKTIINSAKKTLENGNLVIFPTETVYGLGGDATNVGAIKKIYGL
                     KKRPFSNPIICHFKNIEAIEKNFEINAQEYDVGNAFWPGPMTIILKKKKTSSIHPLLS
                     NNLELVGCRIPKHRIALELLASVNFPIAAPSANLATKISSTQTNHLSSELKENAFLID
                     GGKTTLGLESTVVKLNNNKLIILRLGSITEEKIKVKFPELIIENNDFNSNLSPGNQKK
                     HYAPNLPIRINVNKVKKDEGLLNFGKNNLRSNICEYNLSKKADLKEASKNFFHFLHLL
                     DNSNCKGIAVANIPEVGLGKTMNDRLYRASSNNE"
     gene            1388..2830
                     /locus_tag="EYO86_03825"
     CDS             1388..2830
                     /locus_tag="EYO86_03825"
                     /inference="COORDINATES: protein motif:HMM:PF01565.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC42192.1"
                     /translation="MIDYTEPLQIMNRSLIVSKLKNYVKSKNLISNQRDLEKYNKDWR
                     GFYNFNSICAIFPETVEEIQKILIFCNNNNIKIVPQAGNTSLTGASVPSQHDHEVILN
                     INKMNKIIEIDKNNLSVTTEAGVCLDDVKDYADKNNFYFPISLSSSGSCLIGGNIATN
                     AGGINALKYGSMRDNVIGIEVVLADGSLINSMSSMKKNNTGYDLKNIFCGSEGTLGIV
                     TKAILRVYPKPTDYFHCFFAFNSIQENINLFKKIRDVFGDKLESAEIIPDIAIELSIK
                     HGFLTRSFFSKKFSSFLLCKFSLFENKETFEKFFLDKITKFNNKFEDVIFPQSLQQEN
                     NFWKFRDDLVEAYKLEGKYITNDISLPLNNLLKFIDVATKKINKVVYGTQIYSFGHLG
                     DGNIHFNLIEPVNYEGNFNNARSEIYEIVNDLVEDFGGSFSAEHGIGLIKKKSLLKYK
                     SFNELNLMRKIKHALDPNNILNPGKIFDLK"
BASE COUNT         1132 a          428 c          479 g          988 t
ORIGIN      
        1 tactgtcgtt ttttttcttt cctgtcttac taaatttaaa aaaggaacag ttgccatcaa
       61 atcaaggggg ctgggttgaa cgggtacaaa aactttatct gaactcttaa tgatttgaac
      121 agtatcgaat gtaatggatg gtgggctatc tatgattata aaattatatt ttttctttat
      181 ttgctttaag tcttcttcca attttcttac atcgaaagga tatgagtcaa taccagtagg
      241 ctcatctcta tagtattttt ttcttgcttc tagccaatta gtcaggcttc gttgggcatc
      301 agcatcaatt accgcaacct tataattgct gttagcccat aaaactgcaa gattggcaga
      361 tagcgtggtt ttacctgatc cacctttttg atttgcaaaa gtaataattt ttcccatatt
      421 agaaagttgt aaaaaattta gaagataata acaattttat ggttatatga aacatatttt
      481 aaaacaaaaa gcatgaaaac aataattaat tcggcaaaaa aaactttgga aaacggaaat
      541 ttagtcatat ttcccacaga aactgtgtac ggattagggg gggatgcgac aaatgttggg
      601 gcaataaaaa aaatttatgg tcttaaaaaa agaccttttt ccaatcccat aatctgtcac
      661 tttaagaata ttgaagcaat tgaaaaaaat tttgaaataa acgcgcagga gtatgatgta
      721 ggcaacgcct tttggcctgg tccaatgact ataattttaa agaaaaaaaa aacaagctca
      781 atacatccat tgctatcaaa taatttagaa ttggttggat gccgcattcc aaaacatcga
      841 atagctttgg aattgcttgc ttctgtgaat tttccaatcg cagctcctag tgcaaatctt
      901 gcaactaaaa tatcatcaac tcaaactaac catctatctt ctgagttaaa agaaaatgct
      961 tttctaatag acggaggtaa aacaacttta ggtttagaat caactgttgt taaattaaat
     1021 aataataaat taattatatt gcgattaggg agtataactg aagagaaaat aaaagtgaag
     1081 tttcctgaat taataataga aaataatgat ttcaatagta atctttctcc aggaaatcaa
     1141 aaaaagcatt atgctcccaa tctaccaatt agaataaatg taaataaagt taaaaaagat
     1201 gaaggtctgc ttaattttgg gaaaaacaat ttaagatcaa acatttgtga gtataatttg
     1261 agtaaaaaag ctgatttaaa ggaagcaagt aaaaattttt ttcatttttt acatctattg
     1321 gacaactcca attgcaaagg gatagctgta gcaaatattc ctgaggttgg ccttggcaaa
     1381 actatgaatg atcgattata cagagcctct tcaaataatg aataggtcat taatagtttc
     1441 aaaattaaaa aattatgtaa aatctaaaaa ccttatttcc aatcaaagag atcttgaaaa
     1501 atacaacaaa gattggagag gtttttataa ctttaattca atatgtgcta tttttcctga
     1561 aactgtagag gaaattcaaa aaatactaat attttgcaat aataataata ttaagatcgt
     1621 acctcaagcc ggaaacacta gccttacagg cgcttctgtt ccatcacaac atgatcatga
     1681 agtaattctt aatattaata aaatgaataa gataattgaa attgataaaa ataatttatc
     1741 tgtgactact gaagctggag tatgtttgga tgatgtaaaa gattacgctg ataaaaataa
     1801 tttctatttt cctattagtc tctcatcttc tggaagttgt ttaataggag gaaatattgc
     1861 tacaaatgca gggggtataa atgctctaaa atatggctct atgagagaca atgtcattgg
     1921 aattgaggtt gttcttgcag atggctctct gataaactca atgtcttcaa tgaaaaagaa
     1981 taacactggg tatgatctaa aaaatatatt ttgcggatct gaaggaactt taggaattgt
     2041 tacaaaggca atcttgaggg tatacccaaa accaacagat tatttccact gcttctttgc
     2101 attcaactcc atccaagaaa atattaattt atttaaaaaa attagagatg tatttggtga
     2161 taaattagaa agtgcagaaa ttatccctga cattgccatt gaattatcaa taaaacatgg
     2221 atttttaaca cgatcatttt ttagtaaaaa attttctagt tttttacttt gcaaattctc
     2281 tttatttgaa aataaagaga cgtttgaaaa atttttttta gataaaatta caaaattcaa
     2341 taataaattt gaagatgtaa tttttcccca atctcttcaa caagaaaata atttttggaa
     2401 atttagggat gatttggtgg aagcgtataa actagaaggt aaatatatta ctaatgatat
     2461 ttctcttcct ttaaataatt tattaaaatt tattgatgta gcgacaaaaa aaattaacaa
     2521 ggtggtatat ggaacacaaa tatactcttt tggacactta ggagacggca atattcattt
     2581 taatctgatt gaacctgtaa attatgaagg caattttaat aacgctcgtt ctgaaattta
     2641 tgaaatagtt aatgatcttg ttgaagactt tggaggcagt tttagcgctg aacacggtat
     2701 aggactgatt aagaaaaaaa gcttactcaa atataaaagt ttcaatgaac ttaatctaat
     2761 gagaaaaatt aaacacgctc tagatccaaa taatatttta aatcccggaa aaatatttga
     2821 cctaaaataa aagatcgcga agtttgtaat aaaaaatagc tgtcgaataa attggtcttc
     2881 gcactaagct tccacccggt attgataaat gattaatttt gctgaataaa tcaaatcttt
     2941 cattaaagcc gtcaattttc tctgcaatta gtttcccggc aaaagtactt aaagataacc
     3001 catggccaga atagccttgt gcatata
//
LOCUS       DTVV01000321            1057 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1950433, whole genome shotgun sequence.
ACCESSION   DTVV01000321 DTVV01000000
VERSION     DTVV01000321.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1950433"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="EYO86_03830"
     CDS             <1..761
                     /locus_tag="EYO86_03830"
                     /inference="COORDINATES: protein motif:HMM:PF00294.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42193.1"
                     /translation="FDVHDKPLLANYGFDKEIVQFNQEGSTTTEFNNYYSEKNINVRF
                     QKVKFNGFPIDGKLRKSKIYHFGPLLSGDIAMNVYLALKKLSGIKIIDAQGLVRQVRE
                     DNVVWQSNKDVRKILSNFDMIKCDQNELVYITDSKNASKSIELLKRWGLKEIIVTNGS
                     KGSTIYSKSEGEFNIPSFKPTRLKDTTGCGDTYAAIYAYARMKGFPIYEAGYLASAGA
                     SLKIENIGPLSTSIEHIKEKIEKHYKERILHLIA"
     gene            791..>1057
                     /locus_tag="EYO86_03835"
     CDS             791..>1057
                     /locus_tag="EYO86_03835"
                     /inference="COORDINATES: protein motif:HMM:PF00483.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42194.1"
                     /translation="MLMKNIKAAVILAAGKGSRISNTDNYVSKPLLKLYDMTLIERSI
                     FNLYHKLNVKKFYIVTGYKHDTMHDFLEGLKNKINVEINIVFANE"
BASE COUNT          400 a          161 c          178 g          318 t
ORIGIN      
        1 cctttgacgt tcatgataag cctctgcttg caaactatgg ttttgataaa gaaatagtgc
       61 agtttaacca agagggttca actaccacag aatttaataa ttattattct gaaaaaaata
      121 taaatgttcg ttttcaaaaa gtaaaattta atggtttccc aatagacgga aaattaagaa
      181 aatctaaaat ttatcatttt ggtcctttat taagtggaga tatcgcgatg aacgtatacc
      241 ttgccctcaa aaaactatca ggcataaaaa ttattgatgc tcaaggatta gtacggcaag
      301 tgcgagaaga caatgtggtc tggcaatcta ataaagatgt tagaaaaatt ttatctaatt
      361 ttgatatgat taaatgtgat caaaatgaat tggtttatat tacggattct aaaaacgcta
      421 gcaaatctat agaactactc aagcggtggg gacttaaaga aattattgtg acaaatggtt
      481 ccaagggctc tacaatttat agcaagtcag aaggagaatt taatattcca tcatttaagc
      541 ctactaggct taaagataca actgggtgtg gcgacaccta cgcagcaata tatgcctacg
      601 cacgtatgaa aggttttcct atttatgaag caggttatct tgcttccgcg ggggcaagct
      661 taaaaattga aaacatcgga ccgttatcaa cttcaattga acacattaaa gagaaaattg
      721 aaaaacatta caaagaaaga attttgcatt taattgcata atctcttccc aaacatatgg
      781 accataagaa ttgttaatga aaaacattaa agcagcagtc atattagctg ctggaaaagg
      841 aagtcggatc tctaatacag ataattacgt ttctaagccc ttattaaaac tctatgacat
      901 gaccttaatt gaaaggtcta ttttcaattt atatcataaa cttaatgtaa aaaagttcta
      961 catcgttact ggctacaaac atgatacaat gcatgatttt ctagaaggac taaaaaataa
     1021 aattaatgtt gaaataaata tcgtattcgc aaatgaa
//
LOCUS       DTVV01000322            1289 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1951181, whole genome shotgun sequence.
ACCESSION   DTVV01000322 DTVV01000000
VERSION     DTVV01000322.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1951181"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /gene="tatB"
                     /locus_tag="EYO86_03840"
     CDS             complement(<1..310)
                     /gene="tatB"
                     /locus_tag="EYO86_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HIC42195.1"
                     /translation="MFSFGWSEIALTVIVVIIVVGPKEIPNLLKQIGSFSKSLKKISR
                     DFKNSLNELAEENDLKDVKKSISDLKNIKNDLDPTVDFKKEINSIKDTVGSEINSIKD
                     T"
     gene            complement(316..498)
                     /locus_tag="EYO86_03845"
     CDS             complement(316..498)
                     /locus_tag="EYO86_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020049692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIC42196.1"
                     /translation="MTPSIWQLLIVLVIVLLLFGRGKIPQLMGDMAKGIKSFKRGMSD
                     EEKKDENIEKKIDEDK"
     gene            complement(633..>1289)
                     /locus_tag="EYO86_03850"
     CDS             complement(633..>1289)
                     /locus_tag="EYO86_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC42197.1"
                     /translation="GADAAKQLASDGFNISILSSSGKGEALAKELGGIGVTGSNQSND
                     DLKKIVDETTNKWGRIDVLVNSAGHGPRGPILELTDEDWHKGMDTYFMNVVRSTRLVT
                     PIMQQQKSGVIINISTFATFEPDPVFPTSGVFRAGLASFTKLFADKYASDNIRMNNIL
                     PGFIDSLPEKEEFKARIPLKRYGKTNEISEVVCFLASDGAGYITGQNIRVDGGITKSV
                     "
BASE COUNT          427 a          242 c          178 g          442 t
ORIGIN      
        1 ctgtatcttt aatggaatta atttcagaac ctactgtatc tttaatggaa ttaatttcct
       61 ttttaaagtc aactgtggga tctaaatcat ttttgatgtt ctttaaatcc gaaatagatt
      121 tctttacatc tttaaggtca ttttcttcag ctaattcatt taaagaattt ttaaaatcac
      181 gagaaatttt ttttagtgat ttagaaaatg aacctatttg ctttaagaga ttaggtattt
      241 ctttcggtcc aacaacaatt ataactacta taactgtcag tgctatttca gaccaaccaa
      301 aagaaaacat tgagattatt tgtcttcgtc aatctttttt tcaatatttt catctttctt
      361 ttcttcatca ctcatgccac gtttgaagga tttaatgccc ttagccatat ctcccattaa
      421 ttgtggaatt tttccgcgac cgaacaaaag caatacaatt actaaaacta taagaagttg
      481 ccatatacta ggtgtcatac tctattcctt attaaaaatt agctcatatc ttataacgat
      541 aaaaaaatat acttaataaa actagggaaa taaatgaatt tttacaacaa aaaagggatt
      601 tttttagtta ccaaatacaa aaaaagttaa attcaaactg attttgtaat tcctccatct
      661 actcgaatat tttgacctgt tatgtaccca gcaccatctg atgcaagaaa acatactacc
      721 tctgatattt cattagtttt accataacgt ttcaggggaa tccgagcttt gaattcttct
      781 ttttcaggta aactatcaat aaagccagga agaatattgt tcatcctaat attatctgaa
      841 gcatacttgt cagcaaataa ttttgtaaaa gatgcaagac ccgctctgaa aaccccagag
      901 gttgggaaca caggatcagg ttcaaatgtc gcaaaggttg atatgttaat aataacacca
      961 gatttttgtt gttgcatgat gggagtaact aatctggtag aacgcacaac attcatgaag
     1021 tatgtatcca ttcctttatg ccaatcttca tcagtcaact caagtattgg tcctctgggt
     1081 ccgtgaccag cactattaac tagcacatca atacgacccc acttatttgt agtttcatct
     1141 acaatttttt ttaaatcatc atttgactga ttagaaccag taacaccaat gcctccaagt
     1201 tcttttgcca atgcctctcc tttgccagaa gaagatagaa tggaaatatt aaaaccatca
     1261 gatgccaact gtttagcagc atcagctcc
//
LOCUS       DTVV01000323            1078 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1955226, whole genome shotgun sequence.
ACCESSION   DTVV01000323 DTVV01000000
VERSION     DTVV01000323.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1955226"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="EYO86_03855"
     CDS             complement(<1..476)
                     /locus_tag="EYO86_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIC42198.1"
                     /translation="MYLHSKLEDYKGNINIAFIGCGKFISMFLSQYNQLKKIKIDTIV
                     DLDIEGAKSNCIKSGLKKETVEKINFVNSLDSILNRNIEIFIEATGNPVAGTQHACKV
                     IQAKKDIIMVNVEADVLCGKYLSDLAKENNVIYSMAYGDQPSLIIEQIEWARLNGF"
     gene            complement(502..>1078)
                     /locus_tag="EYO86_03860"
     CDS             complement(502..>1078)
                     /locus_tag="EYO86_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline hydroxylase"
                     /protein_id="HIC42199.1"
                     /translation="GKMAFEGDVWQWHQDYGTWVNDDLMPSARAMNVAIFLDEVTEFN
                     GPLLFIPGSHKKGVIKAGHDLTTTSYPLWTIDNNLIKELVDRAGGNKGGIVSPKGPAG
                     SMIIFHSCLVHASNNNLSPFNRISVYLSLCAVSNHIRRFKRKEYIAHRNFAPIKCLPD
                     DCLIKDYEVDLPWEKGVPSSAYQTSLAELLN"
BASE COUNT          344 a          204 c          153 g          377 t
ORIGIN      
        1 aaaaaaccat ttaatctggc ccattcaatt tgttcaataa ttagagaagg ttgatctccg
       61 taggccatcg aataaataac attattttcc tttgctaaat cagataaata ttttccacat
      121 aaaacatctg cttcaacatt caccatgata atatcttttt ttgcctgaat gactttacat
      181 gcgtgttggg tacctgctac cggatttcca gtagcttcaa tgaaaatttc tatgtttcta
      241 tttaatatac tatctaaaga atttacaaaa tttattttct caacggtctc ttttttaaga
      301 ccacttttaa tgcaattgga ttttgctcct tcaatatcta aatcaacgat tgtatcaatt
      361 ttgatttttt tgagttgatt atattgtgat aaaaacattg aaataaattt tccacaacca
      421 ataaatgcaa tatttatgtt gcctttataa tcttctagtt ttgaatgcag gtacataatt
      481 taactagagc aatttattga actaatttaa taattctgca agcgaagttt ggtaagcact
      541 acttggaacg cctttttccc agggtaaatc aacctcatag tctttaatca aacaatcatc
      601 gggtaaacac ttaataggtg caaaattgcg gtgagctata tactctttac gcttaaatct
      661 acggatatga ttactcacag cacagagact taggtatacg cttatcctgt taaaaggaga
      721 tagattatta ttagaagcat gaactagaca actatgaaat ataatcattg atcccgcagg
      781 tccttttgga ctaacgatcc ctcctttatt tcctccagca cgatcaacta attcttttat
      841 aagattatta tcaatcgtcc aaagaggata gcttgttgtg gtaagatcat gcccagcttt
      901 gattactcct tttttatgac tgcctggaat aaataatagt ggtccattaa attctgtaac
      961 ttcatcaaga aaaattgcca cattcatcgc acgtgctgat ggcattaaat catcattgac
     1021 ccatgttcca tagtcttgat gccattgcca aacatcgcct tcaaaagcca ttttgcca
//
LOCUS       DTVV01000324            2908 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1957579, whole genome shotgun sequence.
ACCESSION   DTVV01000324 DTVV01000000
VERSION     DTVV01000324.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1957579"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(60..1319)
                     /locus_tag="EYO86_03865"
     CDS             complement(60..1319)
                     /locus_tag="EYO86_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIC42200.1"
                     /translation="MEKSPEQHKYKNSFLNWIEFRLPIVSYIEKEYKDYPMPKNCNYF
                     WSFGALATILLVVLIVSGIFLAMNYTPHTDMAFDSVERIMRDVSYGWLLRYMHSNGSA
                     FFFIVVYIHILRGMYYGSYKYPRELNWILGVFIYLLMMATSFLGYTLPWGQMSYWGAT
                     VITNLFSAIPLVGDGLKTWILGDYTVGNATLNRFFALHYVLPFVILGVVGLHVVAVHV
                     HGSNNPAGIDIKSKGDTVSFFPYMIVKDTLAVCVCGVAISVIIFFGPNLMAEVDNYIP
                     ADPLVTPSHIVANWYLAPFYAILRAIPDKLGGVVLMFAAILILFVLPWLDTSKTKSCN
                     YRPVYKWFMMLFFVNFFVLGYVGMKPAAGTYLLIARIGLVYYFVFLLILTPFIGKFEK
                     PKKIPASISDILKDNNLSKSEHESGMH"
     gene            1406..1858
                     /locus_tag="EYO86_03870"
     CDS             1406..1858
                     /locus_tag="EYO86_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIC42201.1"
                     /translation="MRIALFEPDIPQNTGNIFRLGACLGIPIDIIEPTGYLFDDKRFQ
                     RSSMDYIKYTEYKRHLDWISFYNWSKENNYRLILLTTKSQKKYINYKFQSNDILLFGR
                     ESAGVTLSVHESVNEQLTIPMVEGLRSINVSSAVALVVGEACRQLNLL"
     gene            complement(1868..2659)
                     /locus_tag="EYO86_03875"
     CDS             complement(1868..2659)
                     /locus_tag="EYO86_03875"
                     /inference="COORDINATES: protein motif:HMM:PF01743.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIC42202.1"
                     /translation="MIQQLINHFLKRKEVKEIITILEKASLELRFVGGCVRNLLMQRK
                     ITDIDLAIKAHPDEIIATLEKNKILYDDFAKKYGSVIAHLNKQKFEITSLREDINQQG
                     RHTNIRYTKNWKIDSQRRDFTINSIYLSSDGTLHDYYNGQEHIADQKLQFIGDVEQRV
                     QEDYLRIFRYYRFLGCFKNPKINKEDEIVLHNYCKRALQHLSNELVRKEILKMFDNPF
                     PIHSFCNNNNKLEKNFWVMATKENFILHNYKPGLTKCLNKIEALF"
     gene            complement(2668..2907)
                     /locus_tag="EYO86_03880"
     CDS             complement(2668..2907)
                     /locus_tag="EYO86_03880"
                     /inference="COORDINATES: protein motif:HMM:PF02569.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42203.1"
                     /translation="MYEHLIELKHNVTSIILDKLKKNLLEVGVNKIDYLEVRNEKELS
                     FASLYEKYRLFVAFYIGNIRVIDNFALHYKVKNKK"
BASE COUNT         1036 a          491 c          414 g          967 t
ORIGIN      
        1 aggctaagat acaaaaactt ggcattaaaa aaagaaaagt taataacaaa actttcatat
       61 taatgcattc cactttcatg ttccgacttt gataaattgt tgtcttttaa aatgtcacta
      121 atacttgctg gaattttctt aggtttctca aattttccta taaaaggagt aagaattaac
      181 aagaaaacaa aataataaac aagtcctatt cttgcaatga gtaaataggt tcctgctgcc
      241 ggtttcattc cgacataacc aagaacaaaa aaattgacaa agaacagcat catgaaccac
      301 ttataaactg gcctataatt acaacttttt gtttttgatg tatccaacca aggtaaaaca
      361 aataaaatca gaatagctgc aaacattaaa acaacaccgc caagtttatc tggaatagca
      421 cggagaatgg cataaaaagg cgctaagtac caattggcga caatatgaga cggagttacc
      481 aatggatcag caggtatata attatctact tccgccataa ggttcggacc aaaaaaaatt
      541 ataactgata tagctacccc gcatacacaa actgcaaggg tatcttttac aatcatgtag
      601 gggaaaaaac taacagtatc gcctttggat tttatgtcta tgcctgcagg attgttggat
      661 ccatgcacat gtacagcaac gacatgcaaa ccaacaacac caagaataac aaaaggcaat
      721 acataatgta gggcaaaaaa acgattgagt gtagcgttgc caactgtata atctcccaat
      781 atccaagttt ttaaaccatc cccaactaga ggaatggcac taaataaatt tgtaattacg
      841 gtagcacccc aataagacat ttgaccccaa ggaagagtgt aaccaagaaa agatgtggcc
      901 atcattaaaa gataaataaa tacccctaat atccagttca attctcttgg gtacttgtaa
      961 gaaccataat acatgcctct taatatatga atgtagacaa caataaaaaa gaatgcggat
     1021 ccattggaat gcatatatcg aagaagccaa ccatagctaa catcacgcat aatacgttcc
     1081 acactatcaa aggccatatc agtatgaggt gtataattca tcgccaagaa aataccgcta
     1141 acaattaaaa caactaatag tattgtcgct aatgcaccga aactccaaaa ataattacaa
     1201 tttttgggca tcggataatc tttatattct ttttcaatat acgatacgat tggaaggcga
     1261 aattctatcc aattaagaaa tgaatttttg tatttatgtt gttcgggtga tttctccata
     1321 ttattgaaaa ccttatatct taaataacat aaaaaatatt gattttttat gtgcccactt
     1381 gtcgcttatt atgttaattt ttaatatgcg aatagcttta tttgaaccag acattcccca
     1441 aaatactgga aacatatttc gtcttggcgc gtgtcttggc atacctattg atattatcga
     1501 accaacgggc tacctctttg atgacaagcg ttttcaaaga tcctctatgg attacatcaa
     1561 gtatactgaa tacaaaaggc atttggactg gatatcattt tataactggt ctaaagaaaa
     1621 taattaccgc ttaatattgc taaccactaa atctcaaaaa aaatatatta attataaatt
     1681 tcaatctaat gatattcttt tatttggaag agaaagtgcc ggtgttaccc tatcagttca
     1741 cgaaagcgtt aatgagcaac taaccattcc aatggttgaa ggtttgcgat ccataaatgt
     1801 tagtagtgca gtagctttag tagttgggga agcgtgtcgt caattaaatt tactttaatt
     1861 tacataatta aaacaaggct tcaattttat tgagacattt agtgagtcca ggtttataat
     1921 tatgtaaaat aaaattttct ttggtagcca taacccaaaa atttttttct aatttgttat
     1981 tattgttaca aaaagaatga attggaaatg gattatcaaa catttttaaa atttctttgc
     2041 gaactaattc attggataaa tgttgaagag ctctcttaca ataattatga agaactatct
     2101 catcttcctt attgattttt ggatttttaa aacatcccaa aaatctataa taacgaaaga
     2161 tacgcaaata gtcttcttga actctttgct caacgtcacc aataaactgt aatttttgat
     2221 cggctatgtg ttcttgacca ttataataat catgtagagt tccatcactt gataaatata
     2281 tagagtttat tgtaaaatct cttctttgtg aatctatctt ccaatttttt gtataacgaa
     2341 tatttgtatg acgtccctgt tgatttatat cttctcgcaa agatgtaatt tcaaattttt
     2401 gtttatttaa atgtgcaatc acagacccat atttttttgc aaaatcatca tatagtattt
     2461 tatttttttc tagtgttgca ataatttcat cagggtgagc tttaatagct agatctatat
     2521 ctgtaatctt tctttgcatt aataaattgc gtacacatcc cccaacaaac cttaattcta
     2581 aagaagcttt ttcaagaatc gtaattattt ctttaacttc ttttcttttt aaaaaatgat
     2641 tgattaattg ttgtatcatt taaggcatta ttttttattt tttactttat agtgcaaggc
     2701 aaaattatca ataacccgaa tattgccaat ataaaaagca acaaacaatc ggtatttttc
     2761 atacaaggaa gcaaacgata attctttttc attccttact tcgagataat caatcttgtt
     2821 cacaccaact tccagcaaat ttttttttaa tttatctaaa attattgatg ttacattgtg
     2881 ttttagttca attaaatgtt cataaata
//
LOCUS       DTVV01000325            2342 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_195833, whole genome shotgun sequence.
ACCESSION   DTVV01000325 DTVV01000000
VERSION     DTVV01000325.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_195833"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="EYO86_03885"
     CDS             complement(<1..666)
                     /locus_tag="EYO86_03885"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIC42204.1"
                     /translation="MQKIKVKNPVVEMNGDEMARILWQFIKDKLILPFLDIDLRYYDL
                     GIKNRDKTNDQITTEAAKAVKEFGIGIKCATITPDKNRVQEFNLKQMWRSPNGTIRDV
                     LGGTIFRQPIICSNVPRIVSNWNKPIVVARHAFGDIYKATDTIIDKPGKLKMVFEPDD
                     GTGKIEKEVYEFKNPGVALSMFNLDNSIIDFARSCFNYGLNLNWPVFLSTKNTILKVY
                     DGRF"
     gene            complement(666..1229)
                     /locus_tag="EYO86_03890"
     CDS             complement(666..1229)
                     /locus_tag="EYO86_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HIC42205.1"
                     /translation="MRKDTYRIMVIKSLTIFCSSSNSLSEDYYIMAEDLGKFLYSKSI
                     TIIYGGGKVGLMGKVSSSSYNMGGKVIGIIPKFLNSKEKINTQTTETIIVKDMPDRKK
                     KLFEMGDAFLILPGGSGTIEEATEVISWKFLGLHNKKIILFNYKKYWDPIINMYDQAE
                     KNNFGTKSLHNLCDLINTLQEFKKIFP"
     gene            1238..1915
                     /locus_tag="EYO86_03895"
     CDS             1238..1915
                     /locus_tag="EYO86_03895"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HIC42206.1"
                     /translation="MKNMQFKIHNQGWVYFISSLIVTIILFTFFPTIGIILGIFTCYI
                     FYFFRDPERSIPLDDVIVSPADGIVTYIGPSRAPIDLETSDQFCKISIFLNIFNVHVN
                     RMPTSGIVKKIKYIHGKFINATLEKSSEDNERNIILIETHNKENIVVTQIAGLIARRI
                     VCEIEENQEVFLGHRFGIIKFGSRVDLYLPAHYKTLITVGQTVIAGETLISNPNNIEN
                     LNKSIKN"
     gene            1916..>2342
                     /locus_tag="EYO86_03900"
     CDS             1916..>2342
                     /locus_tag="EYO86_03900"
                     /inference="COORDINATES: protein motif:HMM:PF01066.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42207.1"
                     /translation="MEKQSYYLSKYIPNFITILSLCCGLTSIRFSINENWKIAVYLII
                     LAAIFDFFDGWFARKLKEGTYLGAELDSLSDFISFGVAPSLLIYYWSLSELKSLGWSI
                     SLFFAICAALRLARFTADIYLTSKPIDPSMYFVGVSSPAA"
BASE COUNT          757 a          371 c          330 g          884 t
ORIGIN      
        1 aaatcgccca tcatatactt ttaatattgt attttttgtt gataaaaaaa caggccaatt
       61 caaattcaat ccataattaa agcaagatct tgcaaaatct ataatggaat tatcaagatt
      121 aaacattgaa agagctacac ccggattttt aaattcataa acttcttttt ctattttacc
      181 tgttccatca tcaggctcaa aaaccatttt taactttcct ggtttatcta taatggtatc
      241 tgtagcttta tatatatcac caaatgcatg tctcgctaca acaattggtt tattccagtt
      301 tgagactatt cgaggaacat ttgagcagat aataggctgt ctaaaaatag tcccccctaa
      361 aacatctcta atggttccat taggtgaacg ccacatttgt tttaagttaa attcttgaac
      421 tctatttttg tcaggagtta ttgtggcaca cttaattcct ataccaaact cttttacagc
      481 tttagcagcc tctgttgtaa tttgatcatt agttttatct ctgtttttta tacctaaatc
      541 ataatatcta agatcaatat ccaaaaaagg aagtatcaac ttatctttta tgaattgcca
      601 taatattctg gccatttcat cgccattcat ttcaacaact ggatttttaa cttttatttt
      661 ttgcattaag gaaatatttt tttaaactct tgcagggtat ttataagatc acataaatta
      721 tgcaaacttt tagttccgaa gttatttttt tctgcttgat catacatatt gattattgga
      781 tcccagtatt ttttataatt aaagagaata atttttttat tatgcaatcc taaaaatttc
      841 caagaaataa cctccgttgc ttcttcaata gtgcctgaac caccgggcaa tattaaaaat
      901 gcatctccca tctcgaataa tttttttttt ctatcaggca tatctttaac aattattgtt
      961 tcagttgttt gggtgttaat cttttctttt gaatttaaaa attttggaat tattccaatc
     1021 acttttcccc ccatattata tgacgaagag gatactttgc ccataagtcc aacttttcca
     1081 cccccataaa ttattgtaat tgatttgctg taaagaaatt tccctaaatc ttctgccatt
     1141 atataataat cttccgaaag ggagtttgat gaagaacaaa aaattgttag agattttata
     1201 accattattc tgtatgtatc ttttctcatt taaatcaatt aaaaatatgc aatttaaaat
     1261 tcacaatcaa ggatgggttt attttatttc atctttaatt gttacaatta tattatttac
     1321 tttttttcca acaattggaa ttattttagg aatttttact tgctatatat tttatttttt
     1381 tcgagaccca gaacgctcga tacctttaga tgatgtaata gtttcacctg cagatggcat
     1441 tgttacgtat atcggaccaa gtagagcacc tatagactta gaaacgagtg atcaattttg
     1501 taaaattagt atttttttaa acatatttaa tgtgcatgta aatagaatgc caacatctgg
     1561 aattgtgaaa aaaattaaat acatacatgg taagtttatt aatgcgacat tagagaaatc
     1621 cagtgaagat aatgaacgaa atattatttt aattgaaacc cataataaag aaaacattgt
     1681 tgttactcaa atagccggtt tgattgcaag acgaattgtt tgcgagatag aagaaaatca
     1741 ggaagtattt ctaggtcatc gttttggtat tataaaattt ggcagcaggg ttgatctgta
     1801 tttacctgca cattacaaaa cattaattac cgttggccaa accgtaattg ctggagagac
     1861 attgatatca aatccaaata atatagaaaa tttaaataaa tcgataaaaa attaaatgga
     1921 aaaacaatct tattatctgt caaaatacat tcccaatttc ataacaatat tatcgttatg
     1981 ttgcggtctt acctcaattc gtttttctat taatgaaaat tggaaaattg ctgtttatct
     2041 aatcatatta gcggcaattt ttgacttttt tgatggatgg tttgctcgaa agttaaaaga
     2101 aggtacttat ttgggtgcag aattagattc tttaagcgat tttatttcat ttggtgtagc
     2161 gccaagtttg cttatttatt attggtcctt atcagagctt aaatcccttg gttggagtat
     2221 atcactgttt tttgctatat gtgcagcctt aagattggct agatttacag ctgatattta
     2281 tttaacatct aaacccattg accctagcat gtactttgta ggggtttctt cccctgcagc
     2341 ag
//
LOCUS       DTVV01000326            2763 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1961213, whole genome shotgun sequence.
ACCESSION   DTVV01000326 DTVV01000000
VERSION     DTVV01000326.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1961213"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="EYO86_03905"
     CDS             complement(<1..1054)
                     /locus_tag="EYO86_03905"
                     /inference="COORDINATES: protein motif:HMM:PF00271.29"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42208.1"
                     /translation="MQNDHNLLAILGPTNTGKTYLAFERLIAYQSGVFGFPLRLLARE
                     NYDKAVARLGIHKVALITGEEKILPKEAKYFFCTVESMPLNIEVECIAIDEVQLAADY
                     ERGHIFTDRILHLRGTFETIFLGSNTIGKILLKLFPFIKIEKRDRYSNLSFLPKKSLS
                     KLNPRSAIIAFNINSVYELAESLRTHKGGAAVVLGSLSPRTRNAQVEVYEDKKVDYLV
                     ATDAIGMGLNLNIHHVSFSSLQKFDGRYTRDLLPAEIGQIAGRAGRYQQDGTFGVMKG
                     AGDLDPLIVQSVEDHCFNDIQKIYWRNSDIDFTSISSAINSFNQFPIKKFFIHKKNAE
                     DEVNFHIISEDTNIIPY"
     gene            complement(1067..>2763)
                     /gene="acnA"
                     /locus_tag="EYO86_03910"
     CDS             complement(1067..>2763)
                     /gene="acnA"
                     /locus_tag="EYO86_03910"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIC42209.1"
                     /translation="FPTDRETINYLRLSGKDKQHLAVVEEYTKVQQLWYDKNSNQEFD
                     ELIEFNLDKVKASVAGPKRPQDKILLEDVPYSFSSIVEKNPNSTREKQQLESGDIVIA
                     AITSCTNTSNPNVMIAAGLVAKKAVDLGLTVKPWVKTSLAPGSKVVSDYLDKSGLTQY
                     LNEIGFNLVGYGCTTCIGNSGPLDGWVSKEINQKKLSVCSVLSGNRNFEGRIHQEIKA
                     NYLASPPLVVAFALAGSIHVNIQKDAIGIGKDGQEVFLKDLWPTTKEIESTLQTSLSS
                     DMFVSRYEEIYKGDENWASIKTVSNDTYEWNDVSTYIKHPPFFDDSSIKTMQDIHEAR
                     ILAMLGDSVTTDHISPAGAIKEDSPAGLYLSERQISKQNFNSYGSRRGNHEIMMRGTF
                     ANIRIRNEIIPNTEGGITHCYANKQDMSIYDAAMEYAAQSIPLVVIAGKEYGTGSSRD
                     WAAKGTRLLGVRAVIAESFERIHRSNLIGMGVLPLEFTNGDNRKKLQLEGNEKITILE
                     MNNITPNKLMHCTIKGESLLNLIDLKCRIDTMKELDYYKAGGILQYVLNLIVDKAS"
BASE COUNT          870 a          530 c          414 g          949 t
ORIGIN      
        1 aataaggaat aatatttgta tcttctgaaa tgatgtgaaa attaacttca tcttcggcgt
       61 tttttttgtg aataaaaaat tttttgatag gaaattgatt aaatgaatta attgcagaac
      121 taatagaagt aaaatcaata tcagagttac gccaatatat tttttgaata tcattgaaac
      181 aatgatcttc tactgactgt acaatcaaag gatcaagatc tcctgcccct ttcataaccc
      241 caaaggttcc atcctgttga tatcgtcctg cgcgccctgc tatttggcct atttcggcgg
      301 gtaataaatc ccgagtatat cgtccatcaa atttttgtaa ggaagaaaat gaaacatgat
      361 gaatatttaa atttaaaccc ataccaatgg catccgtagc cactaaatag tctaccttct
      421 tatcttcata cacttctacc tgtgcattgc gagttctagg acttaaagaa cctagaacga
      481 cagctgcccc acctttatga gtacgtaagg actcagctag ttcatataca gaattaatat
      541 taaaagcaat aatcgctgag cgaggattta atttagatag acttttcttt ggcaaaaaag
      601 agagattaga ataccgatca cgcttttcaa tttttataaa agggaatagt tttaataaaa
      661 tttttcctat tgtatttgat cctaaaaaaa ttgtttcaaa tgttccacgc aaatgaagaa
      721 tacgatcagt aaatatgtgg cctctttcat aatctgctgc taattgtact tcgtcaattg
      781 caatacactc tacctcaata tttaagggca ttgactctac agtacaaaaa aaatacttag
      841 cttctttagg caaaattttt tcttcacctg ttatcaaagc aactttatgt atacctaatc
      901 ttgcaacagc tttgtcataa ttttctcgtg ctaaaagtct taatgggaag ccaaaaacac
      961 cagattgata cgcaataagt cgttcaaaag ctaaataggt ttttcctgta ttagtcggtc
     1021 caagtatggc taataaatta tgatcgttct gcaccaaaga tattaactaa gatgccttat
     1081 caacaattaa attaagaaca tattgcaaaa tacctccggc cttataataa tcaagttctt
     1141 tcattgtatc gatacgacat tttagatcta ttaaatttag taatgactcg cccttaattg
     1201 tacaatgcat caatttatta ggtgtaatgt tattcatttc tagtatagtt attttttcgt
     1261 ttccctctag ctgtaatttt tttctattat ccccatttgt aaattcaagg ggtaacaccc
     1321 ccatacctat taagttagaa cgatgaattc tctcgaatga ctcagcaata acagcacgca
     1381 cccctaacaa acgagttcct tttgcagccc aatctcgtga tgatcctgtt ccgtactctt
     1441 tacctgcaat aacaactaat ggaattgatt gagcagcata ctccatagcc gcatcataaa
     1501 tagacatatc ttgtttattt gcataacagt gcgtaatacc tccttctgta ttcggaataa
     1561 tttcattgcg tatgcgtatg ttagcaaaag tacctcgcat cataatttcg tgattgcccc
     1621 tgcgagatcc ataagaatta aagttttgtt tactgatctg acgctcggat aaatataaac
     1681 cagcagggct atcttctttt attgcaccag caggtgaaat atggtctgtt gttacactat
     1741 cgcctagcat agctaaaatt cgtgcctcat ggatatcttg cattgttttt attgaactat
     1801 catcaaaaaa aggggggtgc tttatgtaag tactaacatc attccattca taagtatcgt
     1861 ttgatactgt tttaattgaa gcccagtttt catccccttt gtaaatttct tcatatcttg
     1921 aaacaaacat atctgatgac aaagaagttt gaagggttga ttcgatttct tttgtggttg
     1981 gccacaagtc ctttaaaaat acctcctgtc catctttccc aatcccaata gcatcttttt
     2041 gaatattaac atgaatactt ccagctaaag caaaggccac aactaatggt ggggacgcta
     2101 aataatttgc ttttatttct tggtgaatgc gtccttcaaa atttctattt ccagacaaca
     2161 cagaacatac agatagtttt ttttgattaa tttcttttga tacccatccg tccaaagggc
     2221 ctgaattacc aatacatgtc gtacagccat atccaaccaa attaaatcca atttcattca
     2281 gatactgtgt caaaccagat ttatctaaat aatcacttac aactttgctt cctggcgcaa
     2341 gactagtttt tacccaaggt tttaccgtta agcctaaatc tactgctttt ttggcaacta
     2401 accctgcagc aatcattaca ttaggattgg atgtgttggt acagctggta attgcagcaa
     2461 ttacaatatc accagattct aattgttgtt tttctcttgt gctatttgga tttttttcaa
     2521 caatagaaga aaatgaataa ggaacatctt ctaataaaat tttatcttga ggccttttag
     2581 gaccggctac agaagctttt actttgtcta aattaaattc aataagttca tcaaattctt
     2641 gattactatt tttatcgtac cacagttgct gtacctttgt atattcttca acaactgcca
     2701 aatgttgttt atcttttcca gataaacgaa gataattgat tgtttctcta tctgttggaa
     2761 aaa
//
LOCUS       DTVV01000327            1124 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1963300, whole genome shotgun sequence.
ACCESSION   DTVV01000327 DTVV01000000
VERSION     DTVV01000327.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1963300"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="EYO86_03915"
     CDS             complement(<1..1091)
                     /locus_tag="EYO86_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42210.1"
                     /translation="MQKAFSNVLSYLVFLSTISIVIISLFAIIFPGFFISIYFPYNVE
                     IVPFEPSIWLIPVSSFSFVFLVLGFVYYKRKLPVQVYKCIQFILNFEISKRITVILGI
                     IIFVFYIGFSVNELSINEDGQFPDDYDILITALQIWPSTESDDIYVSPCSQGDRKCLM
                     VQEQNSRYVRMLLLVSSIEIFQNIKILPFVASISLLIVTFFLTYQISHKRFSGIVALL
                     IILQSHTFLFFDTVAVFENFWILFFLISLYLINRKWQLSSILYLLALFSKAFIFVFFL
                     STIFYIYRARISSRKKIWAMCSYGGVILIILAVFSFGDSVYSDIINISDSEFFLALNT
                     LGYTLRYDVFIILSLLPVTIGLFFTSRRG"
BASE COUNT          481 a          170 c          145 g          328 t
ORIGIN      
        1 aatcctctcc ttgatgtaaa aaataagcca attgttactg gtaatagact caaaataatg
       61 aaaacatcat atcgtaatgt ataacctaaa gtattcaggg ccaaaaagaa ttctgaatca
      121 gagatattta taatatcact atatacactg tctccaaaag aaaaaactgc caaaattatt
      181 aatatcacac caccatatga acacattgcc caaatttttt tccttgaact aatcctagca
      241 cgataaatgt aaaaaattgt tgaaagaaaa aatacaaaaa taaaagcttt tgaaaacaat
      301 gctagtaaat atagaattga cgaaagttgc cattttcgat taattagata taatgatatc
      361 aagaaaaata gaatccaaaa attttcaaat accgcaacag tatcaaagaa taaaaacgta
      421 tgactttgca agattatcag tagagcaact attcctgaaa atcttttgtg agatatttga
      481 tatgttaaga aaaaagttac tattagaaga gatatactag cgacaaaagg taaaatttta
      541 atattttgaa aaatttctat tgaagatact aaaagtagca ttctaacata tcgggaattt
      601 tgttcttgca ccatcaggca ctttcgatcg ccctgcgaac atggggatac atagatatca
      661 tcagattctg ttgatggcca tatttgcaaa gctgttatta atatgtcata atcatcagga
      721 aattgtccat cttcatttat tgaaagctca ttaacagaaa acccaatata gaaaacaaaa
      781 ataattattc ctaggattac cgttatgcgt tttgaaattt caaaattaag aataaactgt
      841 atgcatttgt aaacttgaac aggtaatttc cttttataat acacaaaacc caatacaaga
      901 aaaacaaaac taaatgatga aactggaatt aaccaaatac ttggttcaaa cggaactatc
      961 tcaacattat aaggaaaata aattgatata aaaaaacctg gaaatattat agcaaataaa
     1021 ctaattatca ctatagatat agtagataaa aataccaaat acgacaaaac gttactaaac
     1081 gctttttgca ccaatatgga attaaaatga gaacactact taat
//
LOCUS       DTVV01000328            1383 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1965811, whole genome shotgun sequence.
ACCESSION   DTVV01000328 DTVV01000000
VERSION     DTVV01000328.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1965811"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="EYO86_03920"
     CDS             complement(<1..418)
                     /locus_tag="EYO86_03920"
                     /inference="COORDINATES: protein motif:HMM:PF05724.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC42211.1"
                     /translation="MTYRLPTKKNRKIGYKKLENTGLSKLYDSVYKKSEQKHFTSFLA
                     KGKPSAEHLKIIELFSWKGKKVIDVGCGTGLFAHLVAKKGAEVTGIDFSEQAIKLAQR
                     NHSHRKLHFKKMNVNELKGKFDVIVSIGTLEHVDKPL"
     gene            538..1371
                     /locus_tag="EYO86_03925"
     CDS             538..1371
                     /locus_tag="EYO86_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HIC42212.1"
                     /translation="MVFITAEIGTNHLGDIKIVKQLINVAKAAGCDAIKFQKKSVNKI
                     YSEEFLNSPLESPWGTTQRDMRIHREFSIKQIQEIDKYCKKKNISWYASCWDIDSQLQ
                     MQRFKTKYNKVASAMLVHHKLLEIIAKEKKYTFIATGMSTLKDIEKAVKIFKKYKSPF
                     ELLHCHSSYPMPLEEANLNVIPFLKKKFKCKVGYSGHESGASNVCIPAVMLGASSIER
                     HITLNRTWYGDDQAASLEPDGLKRLVRDIRLIEKILGDGKKRVWRSEIPAQKKLRQIL
                     T"
BASE COUNT          496 a          200 c          235 g          452 t
ORIGIN      
        1 agagtggttt atcaacgtgt tctaatgttc caatagaaac aataacatca aattttcctt
       61 tcagttcatt aacattcatt tttttaaaat gtagtttacg atgactgtgg ttacgttgag
      121 ctagtttgat agcttgctct gaaaaatcaa ttcctgtaac ctctgctcct ttttttgcaa
      181 caagatgagc aaaaagacca gttccacatc caacatcaat aacctttttt cctttccatg
      241 aaaaaagctc aataatttta agatgttctg cggaaggctt tcctttcgct aagaaactag
      301 taaagtgctt ttgttcactt tttttgtaaa cactatcata gagtttggat aatccagtgt
      361 tttcaagttt tttatatcct atttttctat ttttctttgt aggcaatcta taagtcaaat
      421 tatatgctgt tttgataatg tttacgattt taacataagt tacattaaat gattgttttc
      481 ttagtataga taaggaaatg atttatacga tttatcaaat tatttctact ataaaatatg
      541 gttttcatta ctgctgagat tgggacaaat catttgggtg atatcaaaat tgtaaaacag
      601 ctaatcaatg ttgcaaaagc agcaggttgt gatgctataa aatttcaaaa aaagagcgtt
      661 aacaaaattt attcagaaga gtttttgaac tcgcctttag aaagtccttg gggcacaacg
      721 caaagagaca tgagaatcca tagggaattt agtattaaac aaattcaaga aattgataaa
      781 tattgtaaaa agaaaaatat ttcttggtat gcttcgtgtt gggatattga tagccaatta
      841 caaatgcaaa gattcaaaac gaaatacaac aaggttgcat ctgctatgtt ggtgcatcac
      901 aaattactag aaattattgc taaagaaaaa aaatatacat ttatcgcaac tggtatgagt
      961 acattgaaag atatagaaaa agcagtaaaa atttttaaaa aatacaagtc tccttttgaa
     1021 ttgctacact gccatagttc gtatcctatg ccacttgaag aagccaatct aaatgtgatt
     1081 ccatttttaa aaaagaaatt caaatgcaaa gtgggttata gtggacatga aagtggtgca
     1141 tcaaacgttt gtatacctgc tgttatgcta ggtgcctcat ccatagaacg tcacataaca
     1201 ttaaacagaa catggtatgg tgatgatcaa gctgcctcgt tagaaccgga tggattaaaa
     1261 agattagtaa gagatatcag gttaatagaa aaaattcttg gagatggaaa gaaaagggtt
     1321 tggagatctg aaataccagc ccaaaaaaaa ttacggcaga ttttaacgtg acaaaatgaa
     1381 tta
//
LOCUS       DTVV01000329            1132 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1966668, whole genome shotgun sequence.
ACCESSION   DTVV01000329 DTVV01000000
VERSION     DTVV01000329.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1966668"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1132
                     /gene="priA"
                     /locus_tag="EYO86_03930"
     CDS             <1..>1132
                     /gene="priA"
                     /locus_tag="EYO86_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIC42213.1"
                     /translation="TRPFDHAFTYRIKNNQKVKIGSVVSVPFGKKKDQLGMVYELINQ
                     PDKRETFAIKEIGYIFDKIVLNKNIIQFIKWVSDYTLAPIGLVVKLFVINDKIISHNY
                     VTEDDNLFNPNPVKLNKDQTKAADTINKFISQSTAPIVLEGVTGSGKTEVYFDAIEQA
                     IKKKQQALIMLPEISLTPQLEERFHQRFGFLPDVWHSKISDKKRKNIWHHCYHGKPVI
                     IIGARSSLFLPFQNLGLIVVDEEHDASYKQEDNLRYQARDLAVVRAGIEKFSIILSSA
                     TPSLETQNNINKKKYTHVFLPSQFSGLALPKIELVDLQKTKLEKDKWISSKIYSELKN
                     CIDKKEQALLFLNRRGYSPLSLCIECGYRHQCEQCTSWLVMHH"
BASE COUNT          409 a          181 c          190 g          352 t
ORIGIN      
        1 acccgaccgt ttgatcatgc attcacctat aggataaaga acaaccaaaa agttaaaata
       61 ggcagcgttg taagtgttcc gtttggtaaa aaaaaggatc aactaggtat ggtgtatgaa
      121 ttaattaatc aacctgataa gcgtgaaact tttgcaatta aagaaatagg ctatattttt
      181 gacaagatag tattaaataa gaatattatt caatttataa aatgggttag cgattataca
      241 cttgcaccca tcggtcttgt tgttaaactt tttgtaatca atgacaaaat tatctcacat
      301 aattatgtaa cagaggatga taacttattt aatcccaacc cagtaaaatt aaacaaagat
      361 caaaccaaag ctgctgatac aattaataag tttatttccc aatcaacagc gcctattgtg
      421 ttggagggtg tgacaggatc aggaaaaact gaagtttatt ttgatgcaat agaacaagct
      481 attaaaaaaa agcagcaagc attaattatg ttgcctgaaa tatcattgac accacaattg
      541 gaagagcgat ttcatcagcg ttttggtttt ttaccagacg tatggcattc aaaaatttct
      601 gataaaaaga gaaaaaatat atggcatcac tgttatcacg gtaagccagt cattatcatt
      661 ggtgcccgat cctccctatt tttgccattc caaaatcttg gtcttatcgt tgttgatgag
      721 gagcatgatg cttcttataa acaagaagat aatcttcgtt atcaagcaag ggatttagct
      781 gttgtacgtg ccggtataga aaaattttcc attattttat catctgccac accttcttta
      841 gaaacacaaa ataacataaa taaaaaaaag tatactcacg tttttttacc ttcacagttt
      901 tctggattag cccttcctaa aatagagtta gttgacttac aaaaaacaaa attagaaaaa
      961 gataaatgga tttctagtaa aatttattca gagttaaaaa attgtataga taaaaaagag
     1021 caagccctac ttttcttaaa cagaagagga tattctccgt taagtttgtg tattgagtgc
     1081 ggataccggc atcaatgtga acaatgtacg tcttggcttg tcatgcatca ta
//
LOCUS       DTVV01000330            5739 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1968943, whole genome shotgun sequence.
ACCESSION   DTVV01000330 DTVV01000000
VERSION     DTVV01000330.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1968943"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(75..1058)
                     /locus_tag="EYO86_03935"
     CDS             complement(75..1058)
                     /locus_tag="EYO86_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase"
                     /protein_id="HIC42214.1"
                     /translation="MDWIKKSIMATKGVGKNASGKTHNLTEKVQGNYHYTMGPYSKPV
                     MHIKPGDIVVVETRDAFEGKIQKETDKPTEKLEMPFLNPQNGPIMIEGAEKGDAVAVY
                     IESMVPRGENPRGTCCMIPEFGALTGTSYTATLNDPLPEKVRKINLDEKNVYWSDRIT
                     LPYKPHIGTLSVSPEIDSINSLTPDNHGGNMDLPDMGPGSITYLPVRSPGARLFIGDA
                     HACQGDGEVCGVAVEYATVTTIKVDLIKKYTIEWPRLENENMLMAIGSTRPLEDATRI
                     AYRELVRWLTKDYKFNQWDIYMILSQVGKVRLGNFVDPKYTVGAGIEKKYI"
     gene            complement(1084..1806)
                     /locus_tag="EYO86_03940"
     CDS             complement(1084..1806)
                     /locus_tag="EYO86_03940"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC42215.1"
                     /translation="MKENNILKVKNVFSGYGTSNVIKDISFNINESSILAISGRNGVG
                     KSTLLKTIIGIVECKSGEIYFLEKNITKSRPTERSILGIGYVPQGRDIFNSLSVKENI
                     SLPAIARKIKNLNNELDKIYAIFPILGEKQNDLGGSLSGGQQQQLAIARSLIFNPKVL
                     LLDEPSEGIQPSIVSQIADKLNDISSNLKTAIIVIEQNISFISKLNCDCLVIDKGVIF
                     TKILSKDFSNVDTARKILNLKL"
     gene            complement(1806..2507)
                     /locus_tag="EYO86_03945"
     CDS             complement(1806..2507)
                     /locus_tag="EYO86_03945"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC42216.1"
                     /translation="MSESILKINKIKKTFGGFTAVKDVDLKVGRGEIRFIIGPNGAGK
                     STLFKLIIGHLKPDYGDIIFKDEYLNKLEIHQRVKKGIGLKFQAPSIFNEMTVSQNLE
                     IAANETNLEKHDNFIKRFGIFEEKNSLAGDLSHGKKQWLDIALASISNPDLVLLDEPT
                     AGLSFEETKKTGEIIKYLNKQGTTFLIVEHDMEILKQIATAVSVLHLGALFVEGTPES
                     VLKNKDVEKIYLGTS"
     gene            complement(2509..3519)
                     /locus_tag="EYO86_03950"
     CDS             complement(2509..3519)
                     /locus_tag="EYO86_03950"
                     /inference="COORDINATES: protein motif:HMM:PF02653.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC42217.1"
                     /translation="MKNSYFVKNKLQTTTFIILALIPVFVDSYFVNNISFFMIWTFIA
                     LSLSLIWGKGGILSFGQAAFFGLAGYSFGIIGINFAGVYGVGIWSVIIALIITGIFGL
                     IVGYFLFYGGIYDVFVGIVTLSMTLVFETFMSQTAGPEWTVGIARLNGFNGMQGMPSI
                     GISLFGYNVEALDNIGYYVILILAILVLYFLDKLANSKFGLILSASKEDRLRVQSLGY
                     NVKRVHMLTFAISAVIAGMSGILYTNWGGFITPESMGLVSAALPVVYCAASGKNNFFA
                     VFLGTILLGWISQELAINGRQFALIFMGLLLVVATRYFPDGMFLFFINFIKKMAGKII
                     GK"
     gene            complement(3516..4376)
                     /locus_tag="EYO86_03955"
     CDS             complement(3516..4376)
                     /locus_tag="EYO86_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012764437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC42218.1"
                     /translation="MVLLFNVIFQTTDAISFLLLSAIGLAVIFGMMKIINLAHGEFIT
                     VGAYGTVVTAKMGLPLPIAMACGVLVTIIFGFILERLIIRHIYGRPLDSVVATWGISL
                     IASQSFLIIFGPSTEGIKSPFGSFQVGDYSFSEYRVLIIFISFLILILLYFLFMKSKF
                     GTLARATIEKPVTSSALGVDVQKVYTITFCIGAGLAGLTGALYIPTMTAVPSLGQAFM
                     VYSFVTVVTAGASPFVGLVPAAAFLGSFQTGITFFFHATIGLIGLLFAVIMIIRFLPS
                     GFSGLIKRRT"
     gene            complement(4469..5716)
                     /locus_tag="EYO86_03960"
     CDS             complement(4469..5716)
                     /locus_tag="EYO86_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HIC42219.1"
                     /translation="MKNIIKFILSSLFAVVIFAGTSQAADPIKLGILEDETGNFSIAV
                     IPKIHAYELAVKEINEKGGVLGRPLEIIHYDTQSDNTRFQEFARRLIKKDKVDVIFGG
                     FSSASREAIRPIMDKNKQLYFYNNQYEGGVCDSNVFVTGAVPEQQFSTLVPWMMEKYG
                     KNVYNIAADYNFGQISAEWVRQIVKENGGTMVGEEFIPLSVSQFSQTIQKIQKAKPDV
                     LMTLLVGAKQASFYEQQAAAGLGIPMATSINVGQAYEHRRFKPPALENMYVTVNYIEE
                     VHEVIKSGKFHDKSKDFVRRFHELYPNEPYINMESANAYNAINLYAKAVELAGTTDME
                     KVRRALETGISFDGPSGVVKIDPKSHHGSHTIFLVHVGKDHVVTIPKIWENIEPYWLG
                     EAGCDLSKKDDYSQYTPSNPPSN"
BASE COUNT         1848 a         1095 c          797 g         1999 t
ORIGIN      
        1 gaactgcatt tttgatctct ggatcactta ccgttgtatt catatcagtg gcaatagcct
       61 tatcctaaaa tttttcatat atattttttt tcaatccctg cgccaactgt atattttgga
      121 tcaacaaagt tacctaacct tacttttcca acttggctta gaatcatata tatgtcccac
      181 tgattaaatt tataatcttt tgtcaaccat cttactaatt ctctataagc aattctggta
      241 gcatcttcta gcggtcttgt gcttcctatt gccattaaca tattttcgtt ttctaatcta
      301 ggccattcaa ttgtatattt ttttattaaa tctactttaa tagttgttac agttgcatac
      361 tctactgcaa ctccacaaac ttcaccatca ccctggcaag catgagcatc accaatgaac
      421 aatctagctc caggacttct tactggtaga taagtaatac ttccaggtcc catgtctggt
      481 aaatccatat ttccaccatg attatcagga gtaagtgaat taatagaatc tatttctgga
      541 gaaacgctta aagttcctat atgaggtttg taaggtaagg taattctatc tgaccaataa
      601 acattttttt catcaagatt tatttttctt actttttcag gaagaggatc attaagtgtt
      661 gcagtataag atgttcctgt tagagcacca aattcaggaa tcatacaaca agttcctcta
      721 ggattttcac ctcttgggac catactttca atatagaccg caaccgcatc acctttttca
      781 gcaccctcaa tcattatagg tccgttttga ggatttaaga aaggcatttc aagtttttct
      841 gttggtttgt cagtttcttt ctgaattttt ccttcaaatg catctctggt ttctaccacc
      901 actatgtcac ctggttttat atgcattaca ggtttggaat aggggcccat ggtataatgg
      961 taatttcctt gaactttttc tgttaaatta tgcgtttttc cagatgcatt ttttcccaca
     1021 ccctttgttg ccattattga tttttttatc cagtccattt gttcctcact ttatatattt
     1081 attttataat ttcaaattta aaatttttct tgctgtatcg acatttgaaa agtcctttga
     1141 taaaatttta gtaaaaataa ctcctttgtc aataacgagg caatcacaat ttagtttact
     1201 tatgaatgat atattctgtt ctattacgat tatagctgtt ttaagattac tagagatatc
     1261 atttaacttg tccgctattt ggcttacaat cgaaggctgt attccttccg aaggttcatc
     1321 taataataaa acttttggat taaaaattag agatcttgca attgctagtt gctgctgctg
     1381 tcctccgctt aaagatccgc ctagatcatt ttgtttttcc cctaaaattg gaaaaattgc
     1441 atagatttta tcaagttcat tatttaaatt ttttattttt ctagctatag ctggtagaga
     1501 aatattttct ttaacactta acgagttaaa aatatccctt ccttgaggaa catatcctat
     1561 tcccaaaata gatctttctg tagggcgact ttttgttata tttttttcta aaaaatatat
     1621 ttcgcccgat ttacattcca ctattccaat tatagtttta agcaaagtgc ttttgccaac
     1681 accatttctt ccactgatag caagaattga cgattcattg atattaaaac taatatcttt
     1741 tataacatta cttgttccat aacccgaaaa tacatttttt acttttaaaa tattattttc
     1801 tttcattaag aagttcctaa ataaattttt tcaacatctt tatttttcaa gactgattct
     1861 ggtgtacctt caacaaacaa agcgcctaga tgtaatacag atactgcagt tgcaatttgt
     1921 tttaaaattt ccatatcatg ttctactatt aaaaaagtag ttccctgttt gttaaggtat
     1981 ttaattatct ctccagtttt tttagtttct tcaaaagaca atccagctgt tggttcatca
     2041 agtaaaacta aatcagggtt cgatattgaa gctaaagcta tatccaacca ttgttttttt
     2101 ccgtgagata gatcgcctgc caagctattc ttttcctcaa aaataccgaa acgttttata
     2161 aaattatcat gtttttctag atttgtttcg ttagcagcaa tttctaaatt ttgcgaaacg
     2221 gtcatttcat taaaaattga aggtgcttga aatttcaatc caataccttt ttttactctt
     2281 tgatgtattt ctaatttgtt taaatattca tctttgaaaa ttatatctcc ataatcaggt
     2341 tttaaatgtc caataataag tttaaacaac gtgctcttac cggcaccatt aggtccaata
     2401 ataaatctta tttcacctct accaacttta agatcaacat cttttacggc agtgaatcca
     2461 ccaaatgttt tttttatttt gtttattttt aaaattgact cactcatttt atttacctat
     2521 tatttttcca gccatctttt taataaagtt gataaaaaat agaaacatac catcaggaaa
     2581 gtaacgagta gcaaccacca aaagtaagcc cataaaaatt aatgcaaact gccttccgtt
     2641 gatcgcaagc tcttgggata tccatccaag caatattgtt cccaggaata ctgcaaagaa
     2701 attatttttt ccactagctg cgcaataaac cacaggaaga gcagctgata ccaaacccat
     2761 tgattctggg gtaataaaac caccccaatt tgtataaaga atgcctgaca ttccggctat
     2821 aaccgcggat atagcaaaag taagcatatg aacccttttt acgttatacc caagactttg
     2881 aactctaagc ctatcttctt ttgaagctga aagaattaaa ccgaattttg aattggctaa
     2941 tttgtctaag aaatataaga ccaaaattgc caaaataagg attacatagt aacctatatt
     3001 atctaatgcc tcaacattat atccaaataa actaattcct atggatggca ttccttgcat
     3061 gccgttaaat ccgtttagtc ttgctattcc cactgtccat tcaggaccag cagtttgaga
     3121 catgaaagtt tcaaaaacga gtgtcattga tagagttact atgccaacaa aaacatcata
     3181 aataccgcca taaaataaaa agtatccaac tattaaacca aatatgccag taatgattag
     3241 cgcgattata actgaccaaa tccctacacc ataaacacca gcaaaattta ttccaataat
     3301 tccaaagctg tagcctgcta aaccaaaaaa agcagcttgt ccgaaactaa gtattcctcc
     3361 tttaccccaa attaaagata aacttaaagc aataaaagtc catatcatga agaaactaat
     3421 attgtttaca aaataactat caacaaatac gggtatcaag gctaaaatta taaaagttgt
     3481 agtttgtagt ttatttttta caaaatatga atttttcacg ttcttctttt aatcaagcca
     3541 ctaaaacctg atggcaagaa tctaataatc attattacag caaatagaag tccgattaat
     3601 ccgatagtag catgaaaaaa gaaagttata cctgtttgaa aacttcctaa aaacgcagct
     3661 gcaggaacca ggcctacaaa tggacttgcg ccagctgtaa ctaccgtaac gaatgagtat
     3721 accataaaag cttgccccaa actcggaact gcagtcattg ttggaatgta taaagcacca
     3781 gtaagaccag ccaggcccgc accaatacaa aaagttattg tataaacttt ttgtacgtca
     3841 acacccaaag ctgatgacgt aacaggcttt tctattgttg ctcgcgccaa ggttccaaat
     3901 ttacttttca taaacaaaaa atacaaaaga attaaaatta aaaatgatat aaaaattatt
     3961 aatactctat actcagaaaa agaatagtct cctacttgaa atgaaccaaa gggactcttt
     4021 atgccttcag ttgaaggacc gaatataatc aaaaaagatt gtgaagcgat taaacttatt
     4081 ccccaggtgg caaccactga gtctagtgga cgcccataaa tgtgacgaat aataagtctt
     4141 tctaaaataa atccaaaaat aattgttact aaaactccac aggccatagc aataggtaaa
     4201 ggcaggccca ttttggcagt gactactgtt ccataagcac ctacagttat aaattctcca
     4261 tgtgcaagat taattatttt catcattcca aaaattacag ctaacccaat agctgaaaga
     4321 agcaaaaaag aaattgcatc tgttgtttgg aagattacat tgaataatag taccatctat
     4381 ttcacctttt ttagcattat tatagtactt agtaattaag gccctacaaa aaaaaattat
     4441 agggccttaa ctttaattaa tttaaaaatt aatttgatgg tggatttgaa ggggtatatt
     4501 gagaataatc atccttttta gacaaatcac aacctgcttc acctaaccag tatggctcaa
     4561 tgttttccca gatttttgga attgttacaa cgtgatcttt gccaacatgt actaagaaaa
     4621 tagtatgact tccatggtga ctttttggat caatttttac cacaccagat ggtccgtcaa
     4681 agcttatgcc tgtttctaaa gctcttctta ctttttccat atcagtagtt ccagctagct
     4741 caacagcttt ggcataaagg tttattgcgt tgtaagcgtt tgcagattcc atattaatgt
     4801 acggttcatt tggatataat tcatgaaaac gtctcacgaa atctttactt ttatcgtgaa
     4861 attttccaga ctttattacc tcatgaactt cttctatgta gtttacagta acgtacatat
     4921 tttctagagc tggtggttta aatcttctgt gctcataagc ttgaccaacg tttattgaag
     4981 ttgccatcgg tatacctaat ccggcagcag cttgttgttc gtagaatgaa gcttgttttg
     5041 ctcccactaa cagagtcatt aatacatctg gtttagcttt ttggattttt tggattgttt
     5101 gactaaactg agatactgat aatggtataa attcttcccc taccattgta ccaccgtttt
     5161 ctttaacaat ttgtcttacc cactcagctg aaatttgacc aaagttataa tcagctgcaa
     5221 tattgtaaac atttttgcca tatttttcca tcatccaagg aaccagcgta ctaaattgtt
     5281 gttcaggcac tgcacctgta acaaaaacgt tagagtcaca aactccgcct tcatattggt
     5341 tattataaaa ataaagttgt ttatttttat ccattatcgg ccttatagct tctcttgatg
     5401 cactagaaaa accaccaaat attacatcaa ctttatcttt tttaatcaaa cgtcttgcaa
     5461 actcttgaaa acgtgtattg tcagattgtg tatcataatg tattatttct aaaggtcttc
     5521 ctaaaacacc tcctttttcg tttatttcct taacagctaa ttcataagca tgaattttag
     5581 gaattactgc tattgaaaag tttcctgttt catcctctaa tattcccagt ttaattggat
     5641 ctgctgcctg cgaagttcca gcaaatataa ctactgcgaa taaagatgat aaaatgaact
     5701 taattatatt tttcatttgt atctccttaa taaattaga
//
LOCUS       DTVV01000331            2630 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1969433, whole genome shotgun sequence.
ACCESSION   DTVV01000331 DTVV01000000
VERSION     DTVV01000331.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1969433"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            84..482
                     /locus_tag="EYO86_03965"
     CDS             84..482
                     /locus_tag="EYO86_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42220.1"
                     /translation="MNPDSITMTIGVDHTIKKGNELFAMAQGKLELIPDWVSVSGGAS
                     MYIKGRDEFFSNDPSWDKWMSYRKDEYDTRRTAIRQFAEVALHNVNPLKRIGPIPFEL
                     RGGASIPIFTRNTFSDFSAWIQLVVYAQAW"
     gene            complement(656..1408)
                     /locus_tag="EYO86_03970"
     CDS             complement(656..1408)
                     /locus_tag="EYO86_03970"
                     /inference="COORDINATES: protein motif:HMM:PF00753.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIC42221.1"
                     /translation="MSTSVDILINPVTLRFSYHNNELIYMFASNPKQQLDEMEKLMGV
                     STKDGPIFDTNVGFLPVATTTLVRGEKNILVDPGNYHIGFYSILKRALASRNLTYDDI
                     DIIATTHTHSDHAASIVHFRNKPWVIGMKEFYDMEAIEGKEIVNAKKSMMGEIIEISD
                     NNETKIMENMYAITTPGHTSGHISFVVKSDESTVLIAGDQTMIKEEYCDRKFSRWYPE
                     ENLKQLNESLDKAQSYKPDLVIPGHDRAFKPN"
     gene            complement(1411..2622)
                     /locus_tag="EYO86_03975"
     CDS             complement(1411..2622)
                     /locus_tag="EYO86_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42222.1"
                     /translation="MINKDLALFCYPWDVIDEGYDAIINAVKRSGLNSIYITVNYHSG
                     MFFLPHSTKRKIYFPEPGALYFNPSNWHKKHSFQSPISNLTNNWSLFWEELSNKCKQN
                     NIKLCAWMLGTHNSGIGNNYPEMSVHNAWGDPITHSLCPFNSEVVDHFVNLSRDIVNL
                     GVFDKILIESLEYLPLRHGHHHEVIGVDFSADLDFIMSLNFSKKCLEALKQKSVDGEM
                     IKNWVKETTNDYFNKDIKKEIMNWSDFENAIDGQFWKYYQIREESITNINKVVINELR
                     QDKNLKIGLVDFGPLYPLGPNKQRWQNGVNLTSLLPLIDEIHPTFYFADLELFKQKYD
                     IYKNVLENKVDMIPAIRTILPQIANQTDLKNQLKVFNQESAGFSFYNYSFMNYENLDW
                     INNSLNILKDN"
BASE COUNT          876 a          429 c          370 g          955 t
ORIGIN      
        1 taaatatcaa ctggctgatt cgtgcaggta ttcatggaca aacaagagta aatacaccca
       61 tttcttttgt gaattacaat acattgaacc cggacagtat caccatgact attggagttg
      121 atcacacgat taaaaaggga aatgaattgt ttgccatggc tcagggaaaa ttggaactca
      181 ttcctgattg ggtatccgtt tccggcggag catccatgta tataaaagga cgcgatgaat
      241 tcttttccaa tgatccatcc tgggataagt ggatgagtta tcgtaaggat gaatatgata
      301 cgagaagaac agccatcagg caatttgcag aagttgctct tcacaacgtt aatccattga
      361 aacgcattgg cccaataccc tttgaactaa ggggtggtgc atccatacca attttcacca
      421 gaaacacttt cagtgatttt tctgcttgga ttcaattagt cgtttatgct caggcatggt
      481 agattgagat tgaacaaatt ttttaatatt aagtcttaca atatcatatt cagcaaaact
      541 aattacgaaa gtaaaaattg caggatatag atatgtaaca aaaactaaaa tgagagatgg
      601 taaaattaaa cttgtagcaa gtattttttc cttactaaat tttaatcttg atttatcaat
      661 taggtttgaa tgctcgatca tgccccggaa taactaagtc tggtttataa ctttgagctt
      721 tatctaatga ctcattgagt tgttttaagt tttcttctgg ataccatcta gaaaattttc
      781 tgtcacaata ttcttcttta atcattgttt gatccccagc tattaaaaca gtgctttcat
      841 cacttttaac tacaaatgat atatggccac tagtatgacc tggagttgta attgcataca
      901 tattttccat aatctttgtt tcattgttat ctgaaatttc tataatttct cccatcattg
      961 atttttttgc attaactatc tctttacctt caatagcttc catgtcataa aactctttca
     1021 ttccaattac ccaaggttta tttctgaaat ggactattga agctgcatga tctgaatgag
     1081 tatgagtagt agcaataata tcaatatcgt cataagttaa atttctagat gctaaagctc
     1141 tttttaaaat tgaataaaat ccaatgtgat aattacctgg atcaacaagt atattttttt
     1201 ctcctcgtac taaagtagta gtcgcaacag gaaggaaacc aacattagta tcaaaaatag
     1261 gtccatcttt ggtactaaca cccattaatt tttccatttc atcaagttgt tgtttaggat
     1321 ttgaagcaaa catataaatt aattcattgt tatgataact aaatcttaaa gttacaggat
     1381 ttattaaaat atctaccgat gtgctcatat ttaattatcc ttcaaaatat tgagtgaatt
     1441 atttatccaa tctaaatttt cataattcat gaatgaataa ttgtaaaaac taaatccagc
     1501 tgattcttga ttaaatactt taagttgatt ttttaaatcc gtttgattag cgatttgtgg
     1561 aagtattgtt ctaattgctg ggatcatatc aactttattt tctaaaacat ttttatatat
     1621 gtcatatttt tgtttaaaaa gttctaaatc tgcaaaatag aaagttggat gaatttcatc
     1681 aattaatggc aatagagatg ttaaattaac accattttgc catctctgct tatttggacc
     1741 aagtggatat aatggtccaa aatctactaa tccgatcttt aaatttttat cttgtcgtaa
     1801 ttcatttatc acaactttat ttatattggt tatactttcc tctctaattt gatagtattt
     1861 ccagaactga ccatcaattg cattttcaaa atcagaccaa ttcataattt ctttctttat
     1921 atctttatta aaataatcat ttgtggtttc cttgacccaa ttttttatca tttcaccatc
     1981 aacgcttttt tgctttaaag cttctaaaca ctttttacta aaattaagag acattataaa
     2041 atcaagatca gcagaaaaat ctactccaat tacttcatga tgatgaccat gtctaagtgg
     2101 aagatactca agagattcaa ttaaaatttt atcaaatact cctaaattca caatatccct
     2161 tgataagtta acaaagtgat caactacttc agaattaaaa ggacaaagag agtgtgttat
     2221 tgggtcaccc caagcattgt gtacagacat ttcaggataa ttatttccaa ttccagaatt
     2281 gtgtgtacca agcatccaag cacataattt aatattattt tgtttacatt tgtttgataa
     2341 ttcttcccaa aataaactcc aattgtttgt taggttactt attggtgatt gaaaactatg
     2401 ttttttgtgc caattacttg gattaaaata taaggcgcca ggttcaggaa aataaatttt
     2461 tctttttgta ctatgaggta aaaaaaacat tccagaatga taatttactg taatgtaaat
     2521 agaatttaat ccacttctct taactgcatt tattattgca tcataacctt catcaattac
     2581 atcccaggga taacaaaata atgctaaatc tttatttatc attgttacca
//
LOCUS       DTVV01000332            1000 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1970251, whole genome shotgun sequence.
ACCESSION   DTVV01000332 DTVV01000000
VERSION     DTVV01000332.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1970251"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1000)
                     /locus_tag="EYO86_03980"
     CDS             complement(<1..>1000)
                     /locus_tag="EYO86_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase"
                     /protein_id="HIC42223.1"
                     /translation="ICGPITHTPDDNFFAGPAPGLKNFWMLCAASIGIAQGGGAGKYL
                     AQWITYGDADINMLEFEPRRYLSWVNKNYAIEKCKDQYRRMYVTALPNESIEVGRPIK
                     KTPIYKKLKDLGAIYLDSYGWEKPCWFAKPGMREQYSFKRSNAFTYVQKECENIQNNV
                     GILDLSTFAKYEISGDDSETFLNRLCANTIPKKDESIILTHMLNEKGRIQSELTITRL
                     PNNLFYVLSSTASEIRDLDWFNHHLNKDEKIKIKNITQDYGVLVIVGPKSRTTLSQLT
                     SENLNNNNFPWLKGKEILINKIPVRALRINYMGELGWELHHPIKYMESLYDTICEVG"
BASE COUNT          313 a          185 c          130 g          372 t
ORIGIN      
        1 ccaacttcac aaatcgtatc atataaagat tccatgtatt ttattggatg atgtaattcc
       61 caacctaatt ctcccatata attaattcta agagctcgca ccggaatttt atttattaaa
      121 atttctttac cttttaacca tggaaaatta ttgttattta aattttcaga agttaattga
      181 gataatgtag ttcttgattt aggcccaaca attacaagta caccataatc ttgtgtaata
      241 ttttttattt ttatcttctc atctttattt agatgatgat taaaccaatc taaatctcta
      301 atctctgatg cggttgatga caaaacataa aaaagattat taggtaaacg agttatagta
      361 agttcacttt gtatacgtcc cttttcatta agcatatgtg taagaataat gctttcatct
      421 ttctttggaa tagtattggc acacaaacga tttagaaatg tttcactatc atctccagaa
      481 atttcatatt ttgcaaaagt actcaaatcc aaaataccta cgttattttg gatattttcg
      541 cattcctttt gaacataagt aaaagcatta gaccgtttaa aactatactg ttctctcatt
      601 ccaggttttg cgaaccagca aggtttttcc caaccatatg aatctaggta aatagctcct
      661 aaatctttta attttttata aataggtgtt ttttttatcg gtcttccaac ctcaatactt
      721 tcatttggta aggcggttac gtacattctt ctatattgat ccttacattt ctcaatagca
      781 taatttttat taacccagct tagatatctt cttggttcaa actccaacat atttatatct
      841 gcatcaccgt aagttatcca ttgggcaaga tattttcctg ctcctccacc ttgggctatg
      901 cctatacttg cagcgcaaag catccagaaa ttttttaatc caggagctgg tccagcaaaa
      961 aaattatcat ccggagtatg agtaattggt ccacaaatta
//
LOCUS       DTVV01000333            1983 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1970695, whole genome shotgun sequence.
ACCESSION   DTVV01000333 DTVV01000000
VERSION     DTVV01000333.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1970695"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(251..949)
                     /locus_tag="EYO86_03985"
     CDS             complement(251..949)
                     /locus_tag="EYO86_03985"
                     /inference="COORDINATES: protein motif:HMM:PF00814.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42224.1"
                     /translation="MNTNKKNALISSQQNFVNVIMILFFDTVSHLPEFCLIEDNKILF
                     SKKILDDHDDKMSDCIVPIYIELEKKFKLGKKLKYLITVTGPGSYTALRVGIAFLSGL
                     SISMKIPLIGIPCVDLFQYVINTNKKKSTAIYICSSNNQKFMCLYSQDTNECIINKVE
                     KDEIPYIFDTSVVNTLLTNDVSILVDPLLPKNIECKKFDFKKIVLQHVKAILLLPRQE
                     IIEPIYISNNKILS"
     gene            891..1916
                     /locus_tag="EYO86_03990"
     CDS             891..1916
                     /locus_tag="EYO86_03990"
                     /inference="COORDINATES: protein motif:HMM:PF10096.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HIC42225.1"
                     /translation="MTLTKFCWLLISAFFLFVFINTAVANNIKNSAVVFMYHKFGVSK
                     YPSTNITLEQFESHLQEFSLSKYRVLSLEFILDTIINDGQLPNNTVGISVDDADKSFL
                     TTAWPKFKEKNLPVTLFVNTSTIVDNNKNYLSWDQIRQLKNEGVTIGAHSHTHNHLTE
                     LSNTQFIDEIELSNKIFLKELGVIPTLFAYPYGETNEKMMNLLKDYKFKVAFGQHSGV
                     INETSNLYYLPRFSLNEKYGDIDRVKFISQSKGMGVYDFIPSDPHIIENPPYIGFSLL
                     DSHLAPKINCFVFDKKGQVENEIFKFNERIEIRLMRKLHKGRSRLNCTAKDNLDNWRW
                     FGYQFYL"
BASE COUNT          713 a          276 c          265 g          729 t
ORIGIN      
        1 aacataaggg atcaatatct catattctga ttttttttca atagttatta aattttccat
       61 aataaaagcc tctaattgag ctttattaaa caatcctagg aaaaaattta ctttttttta
      121 taattgcaat ctaaattgtt cttgcatcca tcctaaccaa ttgaaataaa agtcataatt
      181 actttttaca aacaatttga tctattcttg tctgtactga tgaaataata tttgtgtttt
      241 taataattat ttaacttaat attttattat tagaaatata aattggctca ataatttcct
      301 gccttggtaa taaaagtatt gccttaacat gttgcaaaac tattttttta aaatcaaatt
      361 ttttacattc aatattttta ggcaataagg gatctactaa tatagaaaca tcattggtca
      421 atagagtgtt aactacggat gtatcaaaaa tataaggtat ctcatccttt tcaactttat
      481 taatgatgca ttcattagtg tcttgagaat aaagacacat aaatttttga ttattagatg
      541 agcaaatata tattgctgtt gatttttttt tattcgtatt gatcacatat tgaaataaat
      601 caacacaagg tatacctatt aaaggaattt tcattgatat acttaaacct gataaaaaag
      661 ctatacctac ccttagagct gtgtacgaac cggggccagt aactgttatt aaatatttta
      721 gtttcttacc taatttaaat tttttttcta actcaatata aatgggtaca atgcaatcac
      781 tcattttatc atcatgatca tctaaaattt tttttgaaaa aagtattttg ttgtcttcaa
      841 taagacaaaa ttctggtaaa tgtgatactg tatcaaaaaa taatatcatt atgacgttaa
      901 caaaattttg ctggctactg ataagtgcat tttttttatt tgtattcatt aatacagcgg
      961 ttgcaaataa tatcaagaat tcagcagttg tgtttatgta tcataagttt ggtgtttcta
     1021 aatatccatc aacaaacatt actttggagc aatttgaatc tcatttacaa gaattttcac
     1081 tttctaaata cagagtatta tcattagaat ttatacttga taccattatt aatgatggcc
     1141 aacttccaaa taatactgtt ggtattagtg ttgacgatgc ggataaatct tttttgacaa
     1201 cagcatggcc aaaattcaaa gaaaaaaacc tgcctgtaac attatttgtt aatacttcca
     1261 cgattgttga caataacaaa aattatttaa gctgggatca aataagacag ctaaaaaatg
     1321 aaggtgttac aattggcgca cactctcata ctcacaatca tttaacggaa ctatcaaaca
     1381 cccagtttat tgatgagatt gaattatcaa ataaaatttt cttaaaagaa cttggtgtaa
     1441 taccaacttt atttgcctat ccttacggtg aaactaatga aaagatgatg aacttactta
     1501 aagattataa atttaaagta gcctttggtc agcattcagg agttattaat gaaacatcca
     1561 atctctatta tttaccacgt ttctctctta atgaaaaata tggtgatatt gatagagtta
     1621 aatttatctc acaatcaaaa ggaatggggg tgtatgattt cattccaagt gatccgcata
     1681 ttattgaaaa ccccccctat attggatttt cacttcttga ttctcatctt gcgccaaaaa
     1741 ttaattgctt tgtttttgac aaaaagggtc aagtagagaa tgagatcttt aaatttaatg
     1801 agaggataga aattcgactt atgagaaaat tacataaagg acgatcacgt cttaattgta
     1861 ctgcaaaaga taatttagac aattggcgat ggtttggcta tcaattttat ttataaagaa
     1921 ttaacaaaat attggtttgt taaatcaaac tgaataaagt tattagtttc aataattgaa
     1981 tta
//
LOCUS       DTVV01000334            1107 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1981107, whole genome shotgun sequence.
ACCESSION   DTVV01000334 DTVV01000000
VERSION     DTVV01000334.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1981107"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            318..>1107
                     /locus_tag="EYO86_03995"
     CDS             318..>1107
                     /locus_tag="EYO86_03995"
                     /inference="COORDINATES: protein motif:HMM:PF09084.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC42226.1"
                     /translation="MKKICNFVSLFLFFISVSAYAVSEASKPVVKVGVLKWGTVNWEL
                     QTLLKKKLDDKNKYKLEVVGLANKNATAIALQGGEVDVIVSDYIWVNRQRAEGANFTF
                     VPHSLTVGGLIARPDSGIESVADLVGKKLGIAGGPVDKSWVILQAYAIEKLGINLRDQ
                     VDTVFAAPAVINEQIIEGKVDAVLNFWHYNARLRAAGMDEIISVKSVLKELDLNPKTP
                     LLGWVFDKGWAEKNPDSITAFLDSSFQTKEILLNQLETWEGLKKK"
BASE COUNT          392 a          122 c          214 g          379 t
ORIGIN      
        1 aaaactgatg gaagagatct aaagttattc atcttccatc aattatataa tgtttgatgc
       61 aagattgcct ttagtgtaag ttggaacact aaaaagtgtg aatatcaata aatatttaaa
      121 taaattctta atcatttttt ctctattata tgttcaagga tatagaatat taacccaaag
      181 ttagaaaata aacactaaaa atcattataa ataattattg taatattttt gtattgtatt
      241 tatacttttt tttgattgaa ttggttttta attaaaaaag ttatgttttt aatatttgta
      301 aattttgaga gagagacatg aaaaagatat gtaattttgt gtctttgttt ctttttttta
      361 tttcagtaag tgcctatgca gtaagtgaag catccaaacc tgttgttaaa gtaggtgttc
      421 ttaaatgggg gactgtaaat tgggaactgc aaactctact gaaaaaaaag ttagatgata
      481 agaataaata caaacttgag gttgtaggtt tagctaacaa aaatgctaca gcaatagcat
      541 tacaaggagg agaagttgat gttattgttt cagattatat ctgggtaaat agacaaagag
      601 ctgagggtgc taattttact tttgttccgc actctttaac tgttggagga ctgattgcca
      661 gacctgatag cggtattgaa agtgttgctg atttagttgg taaaaaactt ggaattgcag
      721 ggggtcctgt agataaaagt tgggtaattt tgcaagctta tgcaatagaa aaattaggta
      781 ttaacttgag agatcaagtt gatactgttt ttgcagcccc agcagttatc aatgaacaaa
      841 taattgaagg caaagtagat gcagttctaa atttttggca ctacaatgca agattaaggg
      901 cagctggtat ggatgagatt atttctgtga aatcagtttt aaaagaatta gatttaaatc
      961 cgaaaacgcc attattagga tgggtttttg ataaagggtg ggctgaaaaa aatcctgatt
     1021 caataacagc ttttcttgat tcatcgtttc agacaaaaga aatacttttg aatcaattag
     1081 aaacctggga aggtcttaag aaaaaaa
//
LOCUS       DTVV01000335            1991 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1987244, whole genome shotgun sequence.
ACCESSION   DTVV01000335 DTVV01000000
VERSION     DTVV01000335.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1987244"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="EYO86_04000"
     CDS             complement(<1..1492)
                     /locus_tag="EYO86_04000"
                     /inference="COORDINATES: protein motif:HMM:PF13361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HIC42227.1"
                     /translation="MVENLKNYLNLLNNQQRKAVVNSKGSTLVLAGAGSGKTRVLTFR
                     ILHLLIEKLAFSNQILAVTFTNKAASEMKIRVSNLLNYPIDKMWLGTFHSLSAKILRA
                     HAELVGLKSNFIIIDSDDQLKLIKQICEQKNIDIKEKPPKYFATVIDNYKNKGIFPQS
                     VKSPKSKKGEENIAIIYKLYQEELLRLNCVDFGDLILFCIKIFKENKEVCSKYQNLFK
                     FILVDEYQDINNVQQQWLEFFYQGHKNICCVGDDDQSIYSWRGADVNILLNFGKNFSK
                     PLIIRLEQNYRSTKNILECASFLIAKNKGRYGKELWSDKDKGEKISIKGFWHTKEESV
                     YVSDEIEKLISNKTPLSEIAILFRVSAHTRSFEDRFINLGLPYKIIGGLRFYERKEIR
                     DVIAYLRLVHNLDDNLALERIINVPKRGIGRTTLGKINSIARNNNISMFNAGSKMIQD
                     STSKVNIEINEFIRKVHKWYNLKKEIDHIELTQLILEDSKYVEYLEQ"
     gene            1552..>1991
                     /locus_tag="EYO86_04005"
     CDS             1552..>1991
                     /locus_tag="EYO86_04005"
                     /inference="COORDINATES: protein motif:HMM:PF04333.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HIC42228.1"
                     /translation="MAIFKILIVFASLFFYNFSLLAVESDTVKTSSSDFETGNFEDEI
                     YDPLEPINRAIFGFNNVADKIVLEPIAKGYRKLPSPIQTGIGNFLSNLKMPLVIVNQL
                     LQGQGKNAGESTGRFVVNSTAGLFGLIDVADKIGLEQTQEDFGQT"
BASE COUNT          655 a          326 c          271 g          739 t
ORIGIN      
        1 cctgctctag atattcaaca tacttagaat cttctaaaat caactgagtt aactcaatat
       61 gatcaatttc tttttttaaa ttataccatt tgtgaacttt ccttataaat tcattgattt
      121 caatattaac ttttgaagtt gaatcttgta tcattttgga tcccgcatta aacattgaaa
      181 tattattgtt tcttgctatt gaattaattt tgcctaatgt cgttctacca attcctcttt
      241 taggaacgtt aataattctc tctagcgcca aattatcatc caaattatgg accaaacgta
      301 aatacgcaat tacatctctt atttcttttc tttcataaaa acgaagacct cctataatct
      361 tatatggtaa accaagattg ataaatcgat cctcaaagga ccgtgtgtgc gctgaaacac
      421 gaaataaaat tgctatttca cttaacggtg tcttgttgga aattaatttt tctatttcat
      481 cgcttacata cacagactct tctttagtgt gccaaaaacc tttgattgaa attttttctc
      541 ctttatcttt gtcgctccaa agttctttac catacctccc cttatttttt gcaattaaaa
      601 aagaagcgca ttccaaaata tttttagttg agcgataatt ttgttccaag cgaataatca
      661 atggtttaga aaaatttttt ccaaaattta ataagatatt aacatcagct cctcgccatg
      721 aataaataga ttggtcatca tctcctacgc aacaaatatt tttatggcct tgataaaaaa
      781 attctaacca ttgctgctgc acattgttta tatcctgata ctcatcaact aaaataaatt
      841 taaacaagtt ttggtattta gaacaaactt ctttgttttc cttaaatatc ttaatgcaaa
      901 ataaaattaa atctccaaaa tcaacgcagt ttaatcgtaa taattcctct tggtatagct
      961 tatatataat tgctatattt tcttctccct ttttactctt gggtgacttt actgattgag
     1021 gaaaaattcc tttgttttta taattatcaa taactgttgc aaaatatttt ggaggttttt
     1081 cctttatatc aatatttttt tgttcacaaa tttgcttaat caattttaat tgatcatccg
     1141 aatcgataat tataaaattt gattttaaac caactaattc agcatgagct cgtaatattt
     1201 tggctgacaa agaatggaag gtacctaacc acattttatc aataggataa ttcaaaagat
     1261 tagaaacgcg tattttcatt tctgatgcag ctttatttgt aaaagtaaca gccaatattt
     1321 gatttgaaaa agctagtttt tcaataagta gatgaagaat tctgaatgtt aaaacacgtg
     1381 tcttgccact tcctgcccca gcaagaacta aggttgatcc tttagagttt acaactgctt
     1441 tccgttgttg attgtttaat aaattaaggt aattttttaa attttcaacc atttttatgc
     1501 tatatttaaa tgtatatagt attataagat tacatcaaat cggaagttaa tatggcaatt
     1561 tttaaaatct taatagtttt cgcttcatta tttttctata atttttcatt attggcagtc
     1621 gagtcagaca ctgtaaaaac tagttcatca gattttgaaa caggaaattt tgaagacgag
     1681 atatatgatc cattagaacc tattaatagg gcaatatttg gttttaataa tgtggctgac
     1741 aaaatcgttt tagaaccgat tgctaaaggt tatagaaaac ttccatcccc tattcaaaca
     1801 ggaattggaa attttttaag caacttaaaa atgcccttag ttattgtaaa ccagcttctt
     1861 cagggacagg gaaaaaatgc tggagaatca actggtaggt ttgtagttaa cagtactgca
     1921 ggtttattcg gacttattga tgttgcagat aaaattggcc tagaacaaac gcaggaggac
     1981 tttggccaaa c
//
LOCUS       DTVV01000336            2301 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1987554, whole genome shotgun sequence.
ACCESSION   DTVV01000336 DTVV01000000
VERSION     DTVV01000336.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1987554"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(11..85)
                     /locus_tag="EYO86_04010"
     tRNA            complement(11..85)
                     /locus_tag="EYO86_04010"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(51..53),aa:Gln,seq:ttg)
     gene            complement(112..948)
                     /locus_tag="EYO86_04015"
     CDS             complement(112..948)
                     /locus_tag="EYO86_04015"
                     /inference="COORDINATES: protein motif:HMM:PF08544.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42229.1"
                     /translation="MQDNETIYTPAKLNLFLKITEKRDDGYHNIRSGITFINLFDVIE
                     IKRSAKMKITYHGPYRPENGFYSNCIIQKTLNFLDLKPRINLEINIKKNIPVQAGLGS
                     ASTNAAGFIQGLDKMQLIKIQEISTYVSLGADIPSFLFANNCLVRGIGDLVSEQYFPK
                     YYFLLVKPSINFSTKEMYKKISTINLQLTNVENENEINEEDTGNDFEKILHNNFRVVQ
                     EILDFLDNLNETIFSRMTGSGSCCYAAFETKKLANEAQVNFQSTYPELWTFVGENNTI
                     NN"
     gene            complement(938..2257)
                     /locus_tag="EYO86_04020"
     CDS             complement(938..2257)
                     /locus_tag="EYO86_04020"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC42230.1"
                     /translation="MNKHIIAFFIALIVFTTSAYSFSSSSYLITNTAIKLFDYKEAYS
                     HLPPNDENLNEIDLNNKLLTLTNLNLIDAAKKIAKQILQTNSLNQEAWMMYLVSSRLK
                     NSSEAFDELALRIKKEEMPLINYVFFNSNGTIKNTKESARSIYEIVKSLLADPKSQDV
                     NYNFILFYLTISNILDPEFYEGYYYSAQIHQFLKNFEQAELYYNMIPSTHNLHIESKK
                     NIALNKSKLNKFTEGEIYLLELISQHPNEDSLLITLADLYRFQKKYDQAIKYYSDFIN
                     AKKYNLSERWRLFYLRGICYERSSQWVLAEIDFLESLKLKSDSPQVLNYLAYGWLEQD
                     LHLDRALQMLQKAYAGNPNSYYIMDSLAWAYYKNNNLDKAVQLMEEVISLAPGEAISL
                     DHLGDIYFALNRKREASYLWKQAKDLAEPDENITESLEKKLEEYYAG"
BASE COUNT          781 a          368 c          322 g          830 t
ORIGIN      
        1 agtgaaaaag tggctggggc ggtaggattc gaacctacga atgccggctc caaaagccgg
       61 tgccttaccg cttggcgacg ccccaagtat taggccttat attaaaaagc tctaattatt
      121 gatagtatta ttttcaccaa caaatgtcca caactcagga taagttgatt gaaaatttac
      181 ttgagcctca ttggctaatt ttttagtttc aaatgcagca taacaacaag aaccactacc
      241 cgtcatacgt gaaaaaattg tttcatttaa attatctaaa aaatctagaa tttcctgaac
      301 aactctaaaa ttattatgta atattttttc aaaatcattt cctgtatctt cttcattaat
      361 ttcattttca ttttcaacat tggtaagctg taaattaatt gttgaaattt ttttatacat
      421 ctctttggtt gaaaaattga ttgaaggttt aactaataaa aaataatatt taggaaaata
      481 ttgttcagaa actaaatctc caatacctct taccaaacaa ttattagcaa ataaaaaact
      541 aggtatatct gctcctaatg aaacataggt ggaaatttct tgaatcttaa ttaactgcat
      601 tttatccaaa ccttgaatga aaccagctgc attagttgat gctgagccaa gcccagcttg
      661 cacaggaata ttttttttaa tattaatttc aaggttaatt ctaggtttta aatcaagaaa
      721 atttagtgtt ttttgaataa tgcagtttga gtaaaaacca ttctctggtc tatagggacc
      781 atggtatgta attttcattt ttgcacttct ttttatctca ataacatcaa acaaattaat
      841 aaaagtaatg cctgaacgaa tattatgata tccatcatca cgcttttcag taattttaag
      901 aaacaaattt aattttgccg gagtgtaaat agtttcatta tcctgcataa tattcttcta
      961 gttttttttc tagagattca gtaatatttt catcaggttc ggccaaatct ttagcttgct
     1021 tccataaata actggcttct ctttttcgat ttaaagcgaa ataaatatcc cctaagtgat
     1081 ccaacgaaat cgcttcacct ggagcaagag aaattacctc ttccattaat tgaactgctt
     1141 tatctaaatt gttattttta taataagccc atgccaaact atccatgata taataactat
     1201 tggggtttcc tgcataagct ttttgtaaca tctgtagtgc acgatccaag tgcagatctt
     1261 gctccaacca tccatatgcc aaatagttta aaacctgagg agagtcagat tttaacttaa
     1321 gagactccaa aaaatctatt tctgctaaaa cccactgcga agatctttca taacaaatac
     1381 ctctaagata gaataaacgc catcgttcag acaaattata tttttttgca ttgataaaat
     1441 cggaataata cttaatggcc tgatcatatt ttttttggaa acgatacaaa tcagctaaag
     1501 taatcaataa actgtcttca ttgggatgct gacttattaa ttctaataaa tatatttctc
     1561 cttcagtaaa cttattcagt ttacttttat tcaatgcaat atttttttta ctttcaatgt
     1621 gaaggttgtg agtagaagga atcatattat agtatagttc tgcttgctca aaatttttta
     1681 aaaattgatg aatttgggca gaataataat atccttcgta aaactcggga tctagaatat
     1741 tgctaattgt taaataaaat aaaataaaat tataatttac atcttgagat ttaggatctg
     1801 ccaataatga tttcacgatt tcatagatag agcgagcgct ttccttagtg ttttttattg
     1861 ttccgttact attaaaaaaa acataattaa taaggggcat ttcttctttc ttgattctga
     1921 gggctaattc atcaaaagct tctgatgaat tttttaatct tgaactaact aaatacatca
     1981 tccatgcctc ttgatttaaa ctattggttt gtagtatttg ttttgctatt tttttagccg
     2041 catcaatcaa attgagatta gttaatgtca gcagtttatt attcaaatct atttcattca
     2101 aattttcatc attcggagga aggtgggaat aagcttcctt atagtcaaaa agcttaattg
     2161 ccgtatttgt aatcagatat gatgaagacg aaaatgaata tgcagaggtt gtaaacacta
     2221 ttaaagctat aaaaaatgca attatatgtt tattcattta caaattcgct tcctaattta
     2281 aaagtattat cattaataat a
//
LOCUS       DTVV01000337            1174 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1991621, whole genome shotgun sequence.
ACCESSION   DTVV01000337 DTVV01000000
VERSION     DTVV01000337.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1991621"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="EYO86_04025"
     CDS             complement(<1..182)
                     /locus_tag="EYO86_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HIC42231.1"
                     /translation="MSNKLNKENSPYLLQHAENPVNWFPWSNEVFTVAKEKDVPIFLS
                     IGYSTCHWCHVMEKESL"
     gene            complement(179..877)
                     /locus_tag="EYO86_04030"
     CDS             complement(179..877)
                     /locus_tag="EYO86_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIC42232.1"
                     /translation="MNEEDRNYQIKQLKQDTKLLKSIGINGLYIQKAKRDKILNLLND
                     KKSQDSFKNLDEIRIAVERCERCPLHKERKNTVFSRGSAMSDVMFIGEGPGKDEDEAG
                     LPFVGRAGQLLNKILEAMKLPIDNVYICNIVKCRPPQNRKPTFDEIDSCSHYLMGQIS
                     FVKPKVIICLGATAAEGILGTKERIKDLRGKFFDKYNAKIIVTYHPAALLRDPSRKKL
                     VWEDMQLVMREIGQ"
     gene            927..>1174
                     /gene="leuS"
                     /locus_tag="EYO86_04035"
     CDS             927..>1174
                     /gene="leuS"
                     /locus_tag="EYO86_04035"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010415.1"
                     /note="leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA
                     synthetase; charges leucine by linking carboxyl group to
                     alpha-phosphate of ATP and then transfers
                     aminoacyl-adenylate to its tRNA; due to the large number
                     of codons that tRNA(Leu) recognizes, the leucyl-tRNA
                     synthetase does not recognize the anticodon loop of the
                     tRNA, but instead recognition is dependent on a conserved
                     discriminator base A37 and a long arm; an editing domain
                     hydrolyzes misformed products; in Methanothermobacter
                     thermautotrophicus this enzyme associates with prolyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIC42233.1"
                     /translation="MEYKHLEIEKKWQKEWEDNGLFVADKNTNKEKYYVLEMFPYPSG
                     RIHMGHVRNYAIGDVVARFKRANGYNVLHPMGWDAFGLP"
BASE COUNT          357 a          219 c          188 g          410 t
ORIGIN      
        1 agagactctt tttccataac atgacaccag tggcatgtcg agtaacctat ggacagaaat
       61 attggcacat ctttttcttt cgctacagta aagacctcgt tggaccaagg gaaccaattt
      121 accggattct ccgcatgttg aagcaaataa ggactattct ctttatttaa tttattgctc
      181 attgtccaat ttctctcatt accaattgca tatcttccca aacaagtttt tttcttgaag
      241 gatcccttaa caatgcggct ggatggtatg taacaataat ttttgcattg tatttatcaa
      301 aaaatttacc tcttaaatcc ttaattcgtt cttttgtgcc aagtattcct tccgctgctg
      361 tagctccaag acaaataatt actttaggtt taacaaatga aatttgtccc attaaatagt
      421 gcgagcagga atcaatttca tcaaaagttg gctttctatt ttgtggtggt ctgcatttaa
      481 cgatattaca aatgtaaaca ttatcaattg gcaacttcat tgcctccaga atcttattta
      541 acaactgtcc ggctctacca acaaatggca atcccgcttc gtcctcatct tttcctggac
      601 cttctccgat aaacataaca tcactcatgg cagaaccacg agaaaaaacg gtattttttc
      661 tttctttgtg caaaggacaa cgttcgcatc gttctaccgc aattctaatt tcgtccagat
      721 tcttaaaact gtcctggctt tttttatcat ttaataaatt taaaattttg tctctcttgg
      781 ctttttgaat ataaagtcca ttgataccaa ttgattttaa aagttttgta tcttgtttta
      841 gttgttttat ttgataattt ctgtcttctt cattcatgat gtatatatta aaattaaact
      901 ataacgtttt tttagcaaaa aaggtcatgg aatacaaaca cttagaaatt gaaaaaaaat
      961 ggcaaaaaga atgggaagat aatggtcttt ttgtagctga taaaaacacc aacaaagaaa
     1021 aatattatgt attagaaatg ttcccttatc cgtcaggaag aattcacatg ggacatgtca
     1081 gaaactacgc catcggtgat gtcgtggcaa ggttcaagag agctaatggc tataatgttt
     1141 tgcatccaat gggttgggat gcttttggtt tgcc
//
LOCUS       DTVV01000338            1270 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1993217, whole genome shotgun sequence.
ACCESSION   DTVV01000338 DTVV01000000
VERSION     DTVV01000338.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1993217"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1270)
                     /locus_tag="EYO86_04040"
     CDS             complement(<1..>1270)
                     /locus_tag="EYO86_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIC42234.1"
                     /translation="SALLQGTHDNYKIDHFKNLIEASSNLTKTKVTKENIASHRVISD
                     HLRASSFLIAEGVLPSNEGRGYVLRRIMRRGMRHSHTLGSKEPIFYKMVPTLIKEMSD
                     SYPELKRAEPLITETLKTEEEKFSSLLNRGIEILNENLNKVKNNSFPGEVAFKLYDTY
                     GFPLDLTADILKNKNIKVDNTGFDREMEKSKKLARANWKGSGDKSLEEKWFKVREQLN
                     PTEFLGYEFDKLEGVVLKISKGKDFVKEAKTGDEVEIITNQTPFYAESGGQVGDQGVI
                     YSNDCKVVIEDTQKKMGDLHVHFGKVDKGSLKINQSVNLEIDVNRRNNARAYHSATHL
                     LHEALRRTLGKHVTQKGSFVSPEKLRFDFSHNKPIEKNEIEKIERYVNDMVNTAADVK
                     TRIMTPKKAVEKGALAMFGEKYGEEVRVLSM"
BASE COUNT          373 a          243 c          151 g          503 t
ORIGIN      
        1 catggataaa actctaacct cttctccata tttttcacca aacatagcta aagctccttt
       61 ttctacagct tttttcggtg tcataattct agttttaaca tccgctgcag tgttaaccat
      121 atcattcaca tatctttcta ttttttcgat ttcatttttt tctataggtt tattatgact
      181 aaaatcaaat ctaagttttt caggactgac aaaagaacct ttttgagtca cgtgcttgcc
      241 aagtgttctt cttaaagctt catgaagtaa atgtgtagct gagtgataag ccctagcatt
      301 atttcttcta tttacgtcaa tttccagatt tacactttga ttaattttta aagaaccttt
      361 gtcaactttt ccgaaatgaa catgaagatc acccattttt ttttgagtat cttcaataac
      421 tactttacaa tcattagaat aaattacgcc ttgatcacca acttgacctc cagactccgc
      481 ataaaaaggt gtttggttag ttattatctc cacctcatca ccagtttttg cttctttcac
      541 aaagtcttta ccttttgata ttttaagaac aactccttca agtttatcaa attcgtaacc
      601 caaaaattct gtaggattaa gttgttctct aactttaaac catttctctt ctaacgattt
      661 atcgccagat cctttccaat tagcacgagc taatttttta cttttttcca tttctctgtc
      721 aaaaccagta ttatcaactt ttatattttt attttttaaa atatctgcag taagatctaa
      781 cggaaaacca taagtatcat ataatttaaa agcaacttca ccaggaaaag aattattttt
      841 tactttattt aaattctcat ttaaaatttc tattcctcta ttaagcaatg aagaaaattt
      901 ttcctcttct gtttttaatg tttctgtaat taaaggttct gctctcttta attctgggta
      961 agaatctgac atttctttaa ttaaggttgg taccatttta taaaaaatcg gttccttgct
     1021 ccctagtgta tgtgaatgtc tcatccctct tctcattatt cttcttaaaa cataacctct
     1081 accttcattt gaaggtaaaa ctccttctgc aatcaaaaat gaactggctc taagatgatc
     1141 tgatattact ctgtgacttg ctatattttc tttggtcact ttagttttag tcaaatttga
     1201 agaagcctca attaaatttt taaagtgatc tatcttgtaa ttatcgtgag tgccttgaag
     1261 aagtgccgaa
//
LOCUS       DTVV01000339            1383 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1994839, whole genome shotgun sequence.
ACCESSION   DTVV01000339 DTVV01000000
VERSION     DTVV01000339.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1994839"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /gene="gatB"
                     /locus_tag="EYO86_04045"
     CDS             <1..1200
                     /gene="gatB"
                     /locus_tag="EYO86_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIC42235.1"
                     /translation="IAFFRKSYFYPDLPKNFQITQLNADGYGNRSIGWGGKVTIDNKE
                     IRIRRIQLEEDPGRLIYEGATERTKITLVDYNRAGTPLVEIVTEPDFENPHQVRNFLN
                     ILSDLIENLGVSDPGLDGAMRADANVSIEGGSKVEIKNIGSFHDLEKAVHFEITRQQS
                     LAEREIEIMQETRHWDDKRKITVSARTKEEEEEYRYHLEADIPWVNISNNVKNQLQIQ
                     MPENISSKKERYVSKYNIAPQVADILASDKYFSDLFENANNETDAKDIANMITTDLMG
                     LVDTREKREQSKLEPKHLADLIECIKLNKITRTSAKLALEKIVKTGKALPQIIEEFDL
                     GHVSDKSTLEGIIGEVLKQETKAVEESKQNPEILNYLVGKVMQKTQGKADPELTLAIL
                     KKNLGID"
BASE COUNT          526 a          206 c          261 g          390 t
ORIGIN      
        1 attgcatttt ttagaaaaag ctatttctat cctgacttgc caaaaaactt tcaaattact
       61 cagctaaatg ctgatggata tggaaataga agcattggtt ggggaggcaa ggttactatt
      121 gataacaagg aaatccgaat aagaagaatt cagctggaag aagatccagg caggttgatt
      181 tacgagggtg ccactgaaag aactaaaata actctagttg attataacag agctggcaca
      241 ccattagttg aaattgtaac tgaaccagat tttgaaaacc cacatcaagt tagaaacttt
      301 ttgaacatac tttcagattt gatagaaaac ttgggtgttt cagatcctgg acttgatggt
      361 gcaatgcgtg ccgatgctaa cgtttcaatt gaaggtgggt caaaagttga gataaaaaat
      421 attggttcat ttcacgattt agaaaaagct gtacattttg agataacaag acaacaaagt
      481 cttgctgaaa gagaaattga aatcatgcaa gaaactagac actgggatga taaacggaaa
      541 attactgttt cagcaagaac aaaagaagaa gaagaagagt acagatatca tcttgaagct
      601 gatattccgt gggtcaatat tagtaacaac gtaaaaaatc aattacaaat tcagatgcct
      661 gagaacatta gctcaaaaaa agaacggtat gtttcaaaat acaatattgc accacaggtt
      721 gcagatattt tagcatctga caaatatttt tctgatctat ttgaaaatgc aaacaacgaa
      781 actgacgcaa aagacattgc aaacatgatt accacagatc ttatgggatt ggttgataca
      841 cgtgaaaaac gtgaacaatc taaactagaa ccaaagcacc ttgcagatct aatcgaatgc
      901 ataaaattga acaagattac cagaacatct gcaaaattag cccttgaaaa aattgtaaaa
      961 actggtaagg ctttaccgca aattattgaa gaatttgact tgggtcatgt ttcagacaaa
     1021 tctacgttag agggaattat tggtgaagtt ctaaaacagg aaacaaaagc tgttgaagaa
     1081 tcaaaacaga atcctgaaat tcttaactat ttggtaggaa aagtcatgca aaaaacacag
     1141 ggaaaggctg acccagaatt aacattagcc attttgaaaa aaaatcttgg tattgactaa
     1201 aaaacgaatt ttagttttgg gcggtgtgtt tgctggattt gatgatgtat tatagttgat
     1261 caactaactt taaaatttta aatgaaattt tttcaaattg agtttttctt tccattgata
     1321 ccattaagac atcatttttt ataggaaagc tcatcacaat gagtttttcc ctaaatgaca
     1381 tgg
//
LOCUS       DTVV01000340            1190 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1997105, whole genome shotgun sequence.
ACCESSION   DTVV01000340 DTVV01000000
VERSION     DTVV01000340.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1997105"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(481..1050)
                     /locus_tag="EYO86_04050"
     CDS             complement(481..1050)
                     /locus_tag="EYO86_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42236.1"
                     /translation="MKVLAILLITVINTAHAFWDGNNPNFSPFGSGTGYNNNAPWGDN
                     SNWNPFISSGNWGPRNDAANLSRYGGRPQSLMQYRKDPRFQPNYPVFPAYQNRINPSN
                     WLTETDFASTLIDIGEHNKDFFVNDNSSPLGLSKSYIRAKAESVEMNKMLLDRSPLAP
                     YKNGANDNIYGNQGYGLSPAALSTNWMNE"
BASE COUNT          411 a          203 c          211 g          365 t
ORIGIN      
        1 attttttata aactcactca ctgttgagtg gtttttaaca ccaggcttaa atgccctgat
       61 gactgcgtat tatacaatat taatattaga agtcaatgat atactaatat atatttacaa
      121 attaatcaca taaaacgcta tagacctact actacaacgt ttagaataaa taataaatat
      181 cagaaataat caaagtaggc ttaaaatcca cagtaaaagg cgcaaattta agaaaaaatc
      241 attgaatttc atctaaattt atggtttata ttgaccttaa atagggtttt atgactaaaa
      301 aagtgcttaa aacatcgaca aaataaagca aggcataggt ttaataaaaa ttaaggttaa
      361 ttttggaatg gttatatact tatatatagg caacatattg tatctataag caattagtat
      421 ttataaggga caaaaaaagg agcgctaagc gctccttttt tataacatca acatgcgacg
      481 ctattcgttc atccaatttg ttgataatgc tgcaggcgag agtccgtaac cctggttgcc
      541 atagatatta tcattagcgc catttttata aggtgctaat ggtgagcggt caagaagcat
      601 tttattcatc tcaactgatt ctgcttttgc acgtatgtaa ctcttagaca ggccgagcgg
      661 tgaagaatta tcattaacaa aaaaatcttt attatgctcg cctatatcta tcaatgtaga
      721 ggcaaagtct gtttctgtta gccagttgct gggattgatt ctattttggt aggctggaaa
      781 cacaggatag ttgggctgga atcttggatc tttcctatat tgcattaatg attgagggcg
      841 gccaccatag cgacttaagt ttgcagcatc atttctaggc ccccaatttc cacttgaaat
      901 aaaaggattc caattagaat tgtcacccca aggagcattg ttgttataac ctgtgcctga
      961 accaaagggt gaaaaattcg ggttgtttcc atcccagaaa gcatgggcag tattaattac
     1021 agttatcaat aggatggcta aaactttcat atttcaaaaa aataaattaa ataaaaaaac
     1081 taattataca taccatatta ttaggaaaaa ataagtggtg ccgatggcca gagtcgaact
     1141 ggcacgagcg tagctcacta ccccctcaag gtagcgcgtc taccaattcc
//
LOCUS       DTVV01000341            1383 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1997161, whole genome shotgun sequence.
ACCESSION   DTVV01000341 DTVV01000000
VERSION     DTVV01000341.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1997161"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="EYO86_04055"
     CDS             <1..1008
                     /locus_tag="EYO86_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIC42237.1"
                     /translation="LTASCGQNPARQASIKAGVPQEITAFTINQVCGSGLRSVALGLQ
                     SIINNEAEIIVAGGQENMTQAPHCFHLRQAGIMGDTEMIDSMIKDGIWDIFNGYHMGV
                     TAENVAEKWQITRDQQDEMALNSQHKAEEAQKNGKFQKEIVSVSVQLKTGTVVVRDDE
                     YPRHGCTLENLKNLKPAFKKDGTVTAGNASGINDGAAAVVLMKLDKAEKLGIKPIAKI
                     ASWATAGVDPAVMGSGPIPASKKALDKAKWKPSDLDLIEANEAFAAQACAVNKEMGWD
                     PEKVNVNGGSIAIGHPIGASGTRILNTLLHEMEKRESKKGLATLCIGGGMGIAMCVER
                     I"
     gene            1012..>1383
                     /locus_tag="EYO86_04060"
     CDS             1012..>1383
                     /locus_tag="EYO86_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_770365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC42238.1"
                     /translation="MSRVALVTGGTRGIGKSISLRLKEEGYTVAASYVSRDEDAQKFT
                     KQTGVHVYKFDVAIYDQCREKIKEIEKDLGPVEILINNAGITRDSMLHKMDVRQWGDV
                     IRTNLDSCFNMSKCVIDSMREK"
BASE COUNT          529 a          192 c          302 g          360 t
ORIGIN      
        1 ctaacagcgt cttgtggcca aaatcctgca agacaagcat ctatcaaagc tggggttcct
       61 caagaaatta ctgcttttac aataaatcaa gtttgtgggt caggattaag atctgttgca
      121 cttggtttac aatctattat caacaatgaa gccgaaatta ttgttgctgg aggtcaagaa
      181 aatatgacac aagcccctca ttgttttcac ctacgacaag ctggaataat gggagataca
      241 gaaatgattg acagcatgat caaagatggc atatgggata tttttaatgg atatcatatg
      301 ggtgtgaccg cagaaaacgt tgcagaaaaa tggcaaataa ctagggatca acaagatgaa
      361 atggctttga attcacaaca caaagcagaa gaagcacaaa aaaatggtaa atttcaaaaa
      421 gaaattgttt ctgtttcagt ccaattaaaa acaggaacag ttgttgttag agatgatgaa
      481 tatcctagac atggatgtac tttagaaaat ctaaaaaact taaaacctgc ttttaaaaaa
      541 gacggaactg ttacagccgg aaacgcttct gggataaatg atggtgcagc cgcagttgtg
      601 ctgatgaaat tagataaagc agaaaaatta ggcataaaac caatagcaaa aatagcatca
      661 tgggccactg ccggagtcga tcctgcagtt atgggaagtg gaccaatacc tgctagtaaa
      721 aaagctcttg ataaagcaaa atggaaacca tctgatttag atttaattga agctaatgaa
      781 gcttttgcag cccaagcatg tgctgtaaat aaagaaatgg gttgggaccc tgaaaaggtt
      841 aatgtaaatg gaggctcaat tgcaatcggt catccaattg gagcttctgg aactaggatt
      901 ttaaatactt tgttgcacga aatggaaaag agagaatcta aaaaaggact agcaacatta
      961 tgtataggtg gaggaatggg catagcaatg tgcgtggaaa gaatataatt tatgtcaaga
     1021 gtcgcattag ttacaggtgg aactagaggt ataggcaaat ctatatcact cagacttaaa
     1081 gaagaaggtt atacagttgc agcaagttat gtttctagag atgaagatgc acaaaaattt
     1141 acgaaacaaa cgggagtgca tgtttataaa tttgatgttg caatttatga ccaatgtaga
     1201 gaaaaaataa aagaaataga aaaagattta ggcccagtag aaattttaat taataatgca
     1261 ggaattacca gagacagtat gttacataaa atggatgtaa gacaatgggg ggatgtaata
     1321 agaacaaatt tagattcatg ttttaatatg tcaaaatgtg taattgatag tatgagagaa
     1381 aaa
//
LOCUS       DTVV01000342            1592 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1998831, whole genome shotgun sequence.
ACCESSION   DTVV01000342 DTVV01000000
VERSION     DTVV01000342.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1998831"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(4..1008)
                     /gene="gap"
                     /locus_tag="EYO86_04065"
     CDS             complement(4..1008)
                     /gene="gap"
                     /locus_tag="EYO86_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_425314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIC42239.1"
                     /translation="MKVAINGFGRIGKLVFRAFLEKKIKNIDIVAINELGNLESSLHL
                     LQYDSIHGKLPIKISKTKNGLKYNNKNIIFCSEGDPNKLPWKKMNIDVVLECSGVFTS
                     KEKAQAHINAGAKKVIISAPASNVDATIVQGVNNKILNKKHSIISNGSCTTNCLAPVA
                     MVLDKVVGIETGFMTTIHSFTGDQRTVDQAHSDLRRARTASSSLIPTSTGAAKALSLV
                     LPQLEGKLDGTAIRVPTPNVSLIDFTFVSKKKTSIEAINNAMVAASKSSNLKNILTVN
                     NLPLVSIDFNHNESSSIFDLTQTQVLNQKFCRILSWYDNEWGFSNRMIDTLKDFKKLL
                     "
     gene            complement(1011..>1592)
                     /locus_tag="EYO86_04070"
     CDS             complement(1011..>1592)
                     /locus_tag="EYO86_04070"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:PF02780.18"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIC42240.1"
                     /translation="EDGPTHQPVEHLASLRAMPNLTVIRPSDIIETMEAWEYALQSSA
                     PTLLVLTRQNLPMLRKKNNVNIVQQGAFPIINHQQYAATILATGSEVEIACQSSVILA
                     KEKINIRVVSFPSWEIFEKQTDEDKLNILGNKPRFAIEAGVINGWERYIPSENFIGMK
                     AFGASGPYKKLYEHFAITSNALVELIKEKMEKN"
BASE COUNT          478 a          280 c          248 g          586 t
ORIGIN      
        1 cacttacaat aattttttaa aatctttgag tgtatcaatc attctattag aaaaccccca
       61 ttcgttatca taccaagaga gaatacgaca aaatttttgg ttaagtactt gtgtttgggt
      121 taaatcaaaa atagaactgc tttcattatg attaaagtca atagacacta acggcaaatt
      181 atttactgta aggatatttt taagatttga tgatttagat gctgcaacca tagcgttgtt
      241 gattgcttct atagaagttt tctttttgct aacaaaagta aaatcaatta gagatacatt
      301 tggagtagga actcttatag ctgttccatc aagtttacct tctaactgtg gcaaaaccaa
      361 acttaaagct tttgcggcac cagttgatgt gggtatcaga gaactagatg ctgttctagc
      421 tcttcgtaaa tcgctatgag cttgatcaac agtacgttga tcacccgtga aactatgtat
      481 agtagtcatg aatcctgttt caatgcctac aaccttatct aaaaccatcg caactggcgc
      541 taggcaattt gttgtgcagg agccattaga tataatagaa tgttttttat tcagaatttt
      601 attatttact ccttgaacaa ttgttgcgtc aacatttgaa gctggtgcgg atataattac
      661 ttttttagcg cccgcattga tatgtgcttg tgccttttct ttagatgtaa aaacaccact
      721 gcactctagt acaacatcaa tattcatttt tttccatgga agtttattcg ggtcaccttc
      781 ggagcaaaat attatatttt tgttattata ttttagccca ttttttgttt tgctaatttt
      841 aataggtaat tttccgtgta tgctatcata ttgcagcaga tgaagactag attccaagtt
      901 tcctaattca ttaatagcaa caatgtcgat atttttaatt tttttttcaa gaaaggctct
      961 aaatactaat ttaccaattc tcccaaaacc atttatagcc actttcatat ttaatttttc
     1021 tccattttct cttttattaa ttcaaccaaa gcattggatg taatggcaaa atgctcatat
     1081 agttttttat atgggccact tgcaccaaat gctttcattc ctataaagtt ttcactagga
     1141 atgtatcttt cccaaccatt tatcacgcct gcttcaatag caaatcttgg cttattacct
     1201 aatatattta atttatcttc atcagtttgt ttttcaaaaa tttcccaact cggaaaggaa
     1261 acaactctta tgtttatttt ttcttttgct aaaataactg agctttgaca agcaatttca
     1321 acttcagagc cagtagccaa tatagttgct gcatattgtt gatgattaat aattggaaaa
     1381 gcaccttgtt gaacaatatt tacattattt ttttttcgaa gcattggaag attttgtctt
     1441 gttagaacta ataatgtggg agccgatgat tgcaatgcgt actcccatgc ctccatagtt
     1501 tcaataatat cgcttggacg aatcacggtt aaatttggca tagctcttaa tgaagcaaga
     1561 tgttcaacag gttggtgagt cggcccatct tc
//
LOCUS       DTVV01000343            2816 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_1999565, whole genome shotgun sequence.
ACCESSION   DTVV01000343 DTVV01000000
VERSION     DTVV01000343.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1999565"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="EYO86_04075"
     CDS             <1..432
                     /locus_tag="EYO86_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase"
                     /protein_id="HIC42241.1"
                     /translation="TKGSVIRFPVQVPGALLSLGDTHAAQGDGEVCGTAIESPMKVVI
                     KINLIKNLSIPSPQYETNGPISNHIDKYGYFATTGIGPDLMEGAKNAVRAMIDLLGKK
                     ENIPANKAYMLCSVCSDLRISEIVDVPNWVVSCYFPKYIFS"
     gene            561..911
                     /locus_tag="EYO86_04080"
     CDS             561..911
                     /locus_tag="EYO86_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIC42242.1"
                     /translation="MSKIIRDDTNQRMSQIVVHNNTIYLSGQVGDKNSKDVVTQTSEV
                     LKKIEKLLLQAGSNKNKILSANIYLSDISYFEEMNKVWDNWLPENCAPARATVEAKLA
                     FPELLVEICIIATK"
     gene            complement(904..1956)
                     /locus_tag="EYO86_04085"
     CDS             complement(904..1956)
                     /locus_tag="EYO86_04085"
                     /inference="COORDINATES: protein motif:HMM:PF00291.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIC42243.1"
                     /translation="MSNNFFPLTAEELFNRINKIPKVKFAMLPTSLEYLPNISKELEI
                     NLYIKRDDCTALAFGGNKTRHLEFIMAKAVSGDYDCILTGAASQSNWCRQAVAAANKL
                     NLESFLVLMHGVKGNAMQGNFLLYNVLGANVDVVEGEDLEEIPKHLDTKYEELIKQGR
                     KPLLIKGGFDYDDTVLAGISYANAMAEIDIQMRKKNFMADHLIVTSANMTQAGCDLGS
                     KILGWSTRVQGISPVFWKLDIKKDIARICNQGAKMLDVNLEFTADMINNDNNYVGEKY
                     GVPTSEGLKAMRLLAKKEGIILDPVYTSKGFSALIDYVKKGIIKKNQNVIYIFTGGSP
                     AVFAYNNEIADSKKIT"
     gene            2094..>2816
                     /locus_tag="EYO86_04090"
     CDS             2094..>2816
                     /locus_tag="EYO86_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIC42244.1"
                     /translation="MRIGVDVGGTNTDAALMDGRTVVDAVKSVTTEDTGLGIIEAVSK
                     ILDKTKILPNQLEAIMIGTTQFTNAFVENKRLEKVAVVRLGHPATISVPPCIDFSINL
                     RKILGENFWILPGGYEFDGRNLGDWTEEKVKKVAHEIASKGIKYIAVSCVFSPINEKQ
                     EIKTAKIIKKIIPEAIITMSHRIGRVGFIERENATIMNSSLGYLANKVVTSFNVALNK
                     LKIKCPFYISQNDGTLMAANLVK"
BASE COUNT          985 a          421 c          441 g          969 t
ORIGIN      
        1 actaaaggtt cagtaattag atttcctgtt caagtgcctg gagcattact ttcacttggt
       61 gatactcatg ctgcacaagg cgacggagaa gtttgtggaa cagctataga aagtccaatg
      121 aaagttgtaa ttaagattaa tcttattaaa aatctatcaa tcccttcccc tcaatatgaa
      181 acaaatggac ctatttccaa tcatatagat aaatatgggt actttgcaac aacaggaatt
      241 ggcccagatt taatggaagg tgcaaaaaat gctgtccgtg ctatgataga tttattagga
      301 aaaaaagaaa atatacctgc taacaaagct tatatgttat gtagcgtgtg ttctgactta
      361 agaattagtg aaattgttga tgttcctaat tgggttgtat cgtgttattt ccctaaatat
      421 atattttctt aataggaaga aatttattta attatttttt cttctttgaa ttttcatcaa
      481 gattactttt aagcgaaaag ggtaggaatg caaacttaga aatatttagg gtttttatgt
      541 taatgtgata atatgtatga atgagtaaaa ttattagaga tgatacaaac caacgaatga
      601 gtcaaatagt ggttcacaat aatactattt atttatctgg tcaggttggg gataagaata
      661 gcaaggatgt tgtcacccaa acttctgaag tattaaagaa gatcgagaaa cttttattgc
      721 aagcaggttc aaataaaaac aagatattat cagctaatat ttatctctct gatatatctt
      781 actttgaaga aatgaataaa gtttgggata attggcttcc agaaaattgc gctccagcaa
      841 gagcaactgt agaagcaaaa ttagctttcc cagaattatt agtggaaatt tgtattatag
      901 caactaagta attttttttg aatccgcaat ttcattattg tatgcaaaaa ccgctgggct
      961 tcctccagta aaaatataaa taacattttg attttttttt ataattcctt ttttgacata
     1021 atcaataaga gcggaaaaac cttttgaggt ataaacagga tctaaaataa taccttcttt
     1081 ttttgcaagt aacctcatcg ctttgagacc ttcagaagtt ggaacaccgt atttttctcc
     1141 tacataatta ttgtcattat taatcatatc tgcagtaaat tctaaattta catcaagcat
     1201 ttttgcacct tgattgcaaa ttcttgcaat atctttttta atatctaatt tccagaatac
     1261 tggagaaatg ccctgaacac gtgtagacca acctaaaatt ttacttccta gatcacaacc
     1321 agcttgagtc atatttgctg aggtcactat taaatgatca gccataaagt ttttttttcg
     1381 catctgaata tcaatttcag ccatcgcatt ggcataacta atgcctgcaa gaacagtatc
     1441 atcataatca aaaccaccct ttatcaacaa aggctttctt ccttgtttaa ttaactcttc
     1501 atatttcgta tctaaatgct ttgggatttc ttcaagatct tctccttcta caacatcaac
     1561 atttgctcct aatacattat ataataaaaa attaccctgc attgcatttc cttttacacc
     1621 atgcataaga actaaaaatg attctaaatt aagcttattt gcagcagcaa cggcctgtcg
     1681 acaccaattg gattgagatg cagcaccagt cagtatacaa tcataatctc ctgatacagc
     1741 tttggccatg ataaattcaa gatgccttgt tttgtttcct ccaaatgcaa gtgccgtgca
     1801 atcatccctt ttaatatata aattaatttc taattctttc gatatatttg gtaaatattc
     1861 taaagaggtg ggcaacatag caaatttaac tttaggaatt ttatttatgc gattaaataa
     1921 ttcttcagca gttaacggaa aaaaattatt tgacattttt ttctttcaaa tttgtttctt
     1981 caatttaaca taaaaattat aatattaaga tatttaattt gcaaagaatt actcaagatt
     2041 gatttaaaaa tattgacaga tatttttaat ttgtattagt tataatataa attatgagaa
     2101 taggtgttga tgtaggcggc acaaatactg atgcagcttt aatggatgga agaacagtag
     2161 ttgatgcggt taaatctgtt actactgaag atactggttt gggcatcatt gaagctgtta
     2221 gtaaaatatt agataaaaca aaaatattac ccaatcaatt agaagctatt atgattggaa
     2281 ctacccaatt tacaaatgca tttgttgaaa ataaacgtct tgaaaaagtt gctgttgtta
     2341 gattaggaca tccagcaaca attagtgttc ctccttgtat tgatttttct attaacctaa
     2401 gaaagatatt aggtgaaaat ttttggatat tacctggcgg atatgaattt gatggtagaa
     2461 atttaggaga ttggactgaa gagaaagtta aaaaagttgc ccatgaaatt gcttcaaagg
     2521 gaattaaata catagcagtt tcttgtgttt tctctcctat taatgaaaaa caagaaatca
     2581 aaactgcaaa aataattaaa aaaattattc ctgaagcgat tattacaatg tctcatagaa
     2641 ttggtagagt tggatttatt gaaagagaaa atgcaacaat tatgaattct tctctaggat
     2701 atttagctaa taaagtggtc acttctttca atgtagcatt aaataaactt aaaattaaat
     2761 gtccatttta cattagtcag aatgatggaa cattaatggc agccaactta gttaaa
//
LOCUS       DTVV01000344            1020 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2001029, whole genome shotgun sequence.
ACCESSION   DTVV01000344 DTVV01000000
VERSION     DTVV01000344.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2001029"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="EYO86_04095"
     CDS             complement(<1..703)
                     /locus_tag="EYO86_04095"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039124.1"
                     /note="catalyzes the oxidation of L-histidinol to
                     L-histidinaldehyde and then to L-histidine in histidine
                     biosynthesis; functions as a dimer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIC42245.1"
                     /translation="MTVKYLKKAIKNPSTDDHKTRSAVQSILNDIEKRKEEGIKEITK
                     KFDKYEGEIVVSKERIEESIKQVDSKTKDDVKFAHERIKRFAEHQLKSMNNNFEVELS
                     KGLFAGQRLIPVDTAGCYIPGGRYAHISSAVMAITPAKVAGVKTIICASPPKDQNGAN
                     PGIIYAANLCGADVMLNLGGIAAIASMAYGCFKNPPVDFLVGAGNQYVAEAKRILFGK
                     VGIDLFAGPTEIAIIA"
     gene            769..>1020
                     /locus_tag="EYO86_04100"
     CDS             769..>1020
                     /locus_tag="EYO86_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HIC42246.1"
                     /translation="MKKNYPRNMIGYGSKTPSIKWPNYAKLALQIVLNYEEGSENCVL
                     HGDKTSETFLSEIIGAQPIKGRHINMESFYEYGSRRGFWR"
BASE COUNT          305 a          188 c          167 g          360 t
ORIGIN      
        1 ctgcaatgat tgcaatttct gtaggtccag caaaaagatc tattcctact tttccaaata
       61 atattcgttt cgcctctgcc acatattgat tgcccgcacc aaccaagaaa tcaacgggag
      121 ggtttttgaa acagccataa gccattgatg caatcgcagc gatacctcct aagtttagca
      181 tgacatcagc tccacaaaga ttggccgcat atataatgcc aggattagct ccattttggt
      241 cttttggtgg actggcgcaa ataatagttt ttactccagc aactttggcc ggtgtaattg
      301 ccataactgc ggacgaaata tgggcataac gtccacctgg aatataacag ccagctgtat
      361 caactggtat taatctttgc cccgcaaaca aaccctttga aagttcaact tcgaaattat
      421 tgttcatact ttttaattga tgctcagcaa atcttttaat tctttcatgc gcaaatttga
      481 catcatcttt tgtttttgaa tcaacttgct taattgattc ttctattctt tctttagata
      541 caactatttc gccctcgtat ttgtcaaatt tttttgtaat ttccttaata ccttcttctt
      601 ttcttttttc aatatcatta agtatgcttt gaacggcaga tcgtgtctta tgatcatccg
      661 tcgatggatt tttaattgct ttttttaaat atttaactgt cataaattac ttgcgagcat
      721 gaattattat gaatgtattt ttattttagt atataaatac tttagtctat gaaaaagaat
      781 tatccaagaa atatgattgg ttatgggtca aaaactccca gtataaaatg gcctaattac
      841 gcaaaattag ctcttcagat agttttaaat tatgaggaag gatcggaaaa ttgcgtttta
      901 catggtgaca aaacctcaga aacttttttg tctgaaatta tcggtgccca accgattaaa
      961 ggaagacaca tcaatatgga atctttttat gaatatggtt caagaagagg tttttggaga
//
LOCUS       DTVV01000345            1102 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2004353, whole genome shotgun sequence.
ACCESSION   DTVV01000345 DTVV01000000
VERSION     DTVV01000345.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2004353"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            147..>1102
                     /locus_tag="EYO86_04105"
     CDS             147..>1102
                     /locus_tag="EYO86_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC42247.1"
                     /translation="MHYLKRLFIFFIFHLLSFPIIAFTLDDVKNRGSLNCGISEIRVG
                     FADIDSDNIWIGFDVDMCRAVAAAVFSDSSQVNFILTTSRSRFPILASGEIDLLSRMT
                     TWTFSRDVNLGFEFAGINFYDGQGFMYKSELGIQTPNDLNGATICIIEGTQMAFNINN
                     FFNKNILTYQSIPVEFDDEAFESFNSGRCDIYSSYMTELANKRIAFNRPEDYSIYSEI
                     ISKEPLGPVVRHGDDQWRDVVSWSLKVMIIAEELNITSKNIDDFLDSDNNEILRLLGM
                     VGAYGDMIELDEKWAYYIIKHVGNYSEVFNKNLGPETRLNLNR"
BASE COUNT          348 a          137 c          189 g          428 t
ORIGIN      
        1 aaaccctttt tgttttattg gatcccttcc tagttttgta agtcttgttt ttcatttcat
       61 atcttaacaa tgatttaaaa aatatttttt gtattatttt tttttcatat tatagtaatt
      121 tttttttaac aatctttagt tgaattatgc actatttaaa acgattattt attttcttta
      181 tatttcatct tttatctttc cctattattg cctttacact tgatgatgtt aaaaatagag
      241 ggtcattaaa ttgtggaatt tctgaaatac gtgtaggatt tgcagatatt gattctgata
      301 atatttggat aggttttgat gtagacatgt gtagagctgt agctgcagca gtattttctg
      361 attctagtca ggttaatttt attctgacta ctagtagatc tagatttcca atattagcat
      421 ctggtgaaat tgatcttctg tcaagaatga ccacatggac tttttctcga gatgtgaacc
      481 taggttttga gtttgcaggt attaattttt atgatggtca aggttttatg tataaatctg
      541 aactaggaat ccaaactcca aatgatttaa atggagctac catatgtata attgaaggta
      601 ctcaaatggc tttcaatatt aataattttt ttaataaaaa tatcttaaca taccaatcaa
      661 ttcctgttga atttgatgat gaggcatttg agagttttaa ttctggaaga tgtgacattt
      721 attcaagtta tatgaccgaa cttgccaata aaagaattgc attcaacaga ccagaagatt
      781 attcaatata ctctgaaatt atttctaaag aacctctagg tcctgttgta cggcatggag
      841 atgatcaatg gcgtgatgtt gtgtcctgga gtttgaaagt tatgattata gcagaagaac
      901 taaatattac ctcgaaaaat attgatgatt ttttggattc agataataat gaaatattga
      961 gattattagg tatggtcggt gcttatggag acatgataga acttgatgaa aaatgggctt
     1021 actatattat taaacacgtt ggtaattaca gtgaggtatt caataaaaat ttaggacctg
     1081 aaacccgatt aaatttgaac cg
//
LOCUS       DTVV01000346            1612 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2006163, whole genome shotgun sequence.
ACCESSION   DTVV01000346 DTVV01000000
VERSION     DTVV01000346.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2006163"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(111..1379)
                     /locus_tag="EYO86_04110"
     CDS             complement(111..1379)
                     /locus_tag="EYO86_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42248.1"
                     /translation="MLVGSFKKILFFVLIFYSLQLFAKDFDIEVSGNVQSGYKLYDHY
                     SFSPYFDQNKTQDFSAIARIIFEGVIQDQYSYELHAVQAYNYSNLKTGIRGRNISMLS
                     VDLSDDWINDTDRSAHSYIDRANIKYSTQNLDFQMGRLAASFGKPTFWNLFDYYGSAY
                     LGQEYKAGIDALKIDKAFGNFSGITAVINEQNVLSSSGSYLENNAAQSYQWLGANKKI
                     GLLLKGYTNYQDVDYALLYKREPEGHRLGLEIDGEMGTINLYNEITYLWGSEKISMPS
                     SYQGNLIKNHFMNVLGASYHFDNNLDVTAEHLFNEMGDPDNLDASDIRRKNGVSTSLN
                     NNLSAILMSYEFTPLLMSKYDAKLAWGDSSHQHNFSFIKSVTENIDLTIGGQLNFGNR
                     PNGSNWQNPKLQSEYGSLSNIYYLELKRYF"
     gene            complement(1389..>1612)
                     /locus_tag="EYO86_04115"
     CDS             complement(1389..>1612)
                     /locus_tag="EYO86_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC42249.1"
                     /translation="VLADEITANVDSKTAQSLLELMVALNKNNNTTFLFSTHDPSVIK
                     FAKKIIILKDGIINSEKASSEEIERFTHK"
BASE COUNT          559 a          271 c          236 g          546 t
ORIGIN      
        1 aattgacaga ctagagatag cgaaaaaaag aagagtatga aaaaagcagc agaaaaaaga
       61 ttaacaaagc aaatagctaa gttttttcat gcttacatta atataaatac ttaaaaatat
      121 cgctttaatt ctaaataata aatatttgat aaacttccat attcactttg tagttttggg
      181 ttttgccagt ttgagccatt tggtctgttg ccaaaattaa gttgaccacc aattgttaaa
      241 tcaatgtttt ctgttaccga tttaataaat gaaaagttgt gctgatgaga agaatcaccc
      301 caagctaatt ttgcatcata tttactcatc agtaaaggag taaactcata actcattaat
      361 attgcgctca aattattatt aagagatgta ctgacgccgt ttttacgtcg tatatctgag
      421 gcatccaaat tatcaggatc ccccatttca ttgaagagat gttctgcagt cacatccaag
      481 ttattatcaa aatgataact agcacccaaa acgttcatga aatgattttt gattaaatta
      541 ccttgatatg aacttggcat tgaaattttt tctgatcccc ataaatatgt aatttcatta
      601 tatagattga tcgttcccat ttctccgtct atctcaagcc ctaaacgatg accctctggc
      661 tcccttttgt ataagagtgc ataatcaaca tcttgataat tagtataacc tttcaataac
      721 aaaccaatct tcttgtttgc tcctagccat tgataggatt gggctgcatt attttccaaa
      781 taactccctg aggaagaaag aacattttgt tcatttataa cagcagtaat tccgctaaag
      841 ttaccgaacg ctttatctat ctttagagca tcaattcctg ctttgtattc ttgaccaaga
      901 taagcgcttc cataataatc aaacagattc caaaaagttg gtttgccaaa actagctgct
      961 agtcttccca tttgaaaatc taggttttga gtagaatatt ttatattagc gcgatcaatg
     1021 taagaatgcg cagaacgatc agtatcattg atccagtcat cactaaggtc aacagataac
     1081 atagaaatat ttcttcctcg aatgcctgtt tttaaattag aatagttgta agcttgtaca
     1141 gcatgcaatt cgtaagagta ttggtcttga atgactcctt caaaaatgat tcttgctatt
     1201 gctgaaaaat cctgggtttt attttgatca aaatatggag aaaaactata atgatcgtaa
     1261 agtttataac cagattgcac atttccacta acttcaatat caaaatcttt tgcaaataat
     1321 tgtaaagagt aaaatattag aacaaaaaat agtatttttt taaaactgcc aacaagcatc
     1381 aaaaaaaatc atttatgagt gaatcgctct atttcttctg aagaagcctt ttcactattg
     1441 ataattccat cttttaaaat aataattttt tttgcaaatt tgataacaga tggatcatga
     1501 gtagaaaata aaaaagttgt attgttattt ttatttaatg ccaccataag ttccaaaagt
     1561 gattgagctg tcttagaatc tacattggct gttatttcat cagctaagac ta
//
LOCUS       DTVV01000347            1213 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2009013, whole genome shotgun sequence.
ACCESSION   DTVV01000347 DTVV01000000
VERSION     DTVV01000347.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2009013"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="EYO86_04120"
     CDS             complement(<1..868)
                     /locus_tag="EYO86_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate phosphomutase"
                     /protein_id="HIC42250.1"
                     /translation="MTSNNLIKKLREGEMVKACGAFDVMSAKLIEKVTPSFDAIWASG
                     FGISTARALPDAGIITMTELLDAVRFMAEACDIPVIADCDTGYGGPHNVRHLVRKFED
                     AGIGGICIEDKIFPKINSLFKDGKHNLLPKKDYLAKIIAAKNAKKNSDFLIIARTEAL
                     IAGLGMEEALERAEAYEKAGADIIFLHSRKDTPDEIFEFCERRNGKAPIMIVPTAYPT
                     VTLDELKSHGVRMVVYAHQTTLAAFAAVSDVIKQMSKVKSLSDLNVKLASMDDVFKLQ
                     RMQKIRDFDNFIE"
BASE COUNT          381 a          228 c          151 g          453 t
ORIGIN      
        1 cttctataaa gttatcaaag tctctaattt tctgcattct ttgtagtttg aaaacatcat
       61 ccatggaagc caattttacg tttaaatcac taaggctttt gacctttgac atttgtttaa
      121 taacatctga cactgcagca aacgctgcaa gtgtagtttg gtgggcatat actaccattc
      181 ttactccatg cgatttcaat tcatctaaag ttacagtagg atacgctgtt ggaactatca
      241 taattggcgc ttttccattt cgtcgttcac aaaactcaaa aatttcatca ggagtatctt
      301 ttcgtgagtg aagaaagata atatcagcac cagctttttc atacgcttcc gccctctcta
      361 atgcttcctc cattcctaaa cctgcaataa gagcctcggt tcttgctata attaagaaat
      421 cagaattttt tttcgcattt tttgcagcta ttatctttgc caaataatct tttttaggaa
      481 ggagattatg ttttccatct ttaaacaaac tatttatttt tggaaagatt ttatcttcaa
      541 tacatatacc tcctattcca gcatcttcaa attttcttac caaatgacga acgttatgag
      601 gtccaccata acctgtatca caatctgcaa tcacagggat atcacatgcc tccgccatga
      661 atctaactgc atctaagagt tcagtcatgg tgattatacc agcgtcaggt aacgctcttg
      721 ctgtagatat tccaaatcca cttgcccaaa ttgcatcaaa actaggtgta accttttcaa
      781 taagttttgc agacataaca tcaaatgcac cacaagcttt taccatttcc ccttctcgca
      841 gtttttttat cagattgtta ctggtcatat gaaatatact actttcatct atttttaaat
      901 ataaaaaata tcctacttta taatttgtta gtgtttaaaa ttaggtgtat aaagatatat
      961 tctaacctaa atattttgta actatatata tattccttaa aaaagtcagc cgggatttgt
     1021 tgtagaattt caaaatttac ttttaaatta acttttgtag tatactttta acagtttttt
     1081 tcttcaattg tattaaccaa actatatttt tcatttagat taagtattaa aaacattcca
     1141 caaaatttaa cttaaaatgt tttcaatgct ttcctctgaa tttattaatt aaaaaattaa
     1201 aaattttttt aga
//
LOCUS       DTVV01000348            1576 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2010067, whole genome shotgun sequence.
ACCESSION   DTVV01000348 DTVV01000000
VERSION     DTVV01000348.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2010067"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="EYO86_04125"
     CDS             <1..576
                     /locus_tag="EYO86_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIC42251.1"
                     /translation="VFVDGGSTDGTVEKAKELGFDVIHQKNKGEGNACRIGTDATQSD
                     YVMFFSPDGNDLPEDIPKLIEKTKEGHDVVHISRFGKNSVSEDANWIERFGNYMFTFL
                     VNTFFGGNYTDALNGFRIIKRELWDELKTDAQYLNVEQQTCIRLAKRKIPIYEIESIE
                     PNRIGGERKMRPLPVGGMLSYQIIKEFIFWK"
     gene            578..>1576
                     /locus_tag="EYO86_04130"
     CDS             578..>1576
                     /locus_tag="EYO86_04130"
                     /inference="COORDINATES: protein motif:HMM:PF13231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42252.1"
                     /translation="MDKKQIIFSLIIICLISLGLKLYLVDFSIPVNSDNLGYTLNAIA
                     HTNGDFSQSSHRGMGWSLFVSMFFGFIDSENFFDYSNTIRALSIGVATFSIPMMYVVG
                     RKFFDERYSIVLASLFAFEPHLNYNSGFGLTEPLFHLAIIGSFYFILNKNTRFVLISL
                     FLAGIAYWIRINGVFVFIVITIIYFITLRGSPNLFRNYGLGVVLFLLVISPVLLERNE
                     EFGDPFYSEFKGIIFAGSPELFFSEIHNIVATEKTYSPFDYIEENGLPSFIQTFILQG
                     FYNSLQVVSSLAFPFLFILIPFGIIFSFRAFDQNPNYIKANWIFILVSLASLTIVMS"
BASE COUNT          509 a          201 c          287 g          579 t
ORIGIN      
        1 gtgttcgttg atggtggttc aactgacgga actgtagaaa aggctaaaga attaggattt
       61 gacgtaattc atcaaaaaaa caaaggagag ggaaatgcct gccgaattgg tacagatgca
      121 acacaatcag actatgttat gttttttagt cctgatggaa atgatttgcc tgaagatata
      181 cctaaattga ttgagaagac taaagaaggt cacgatgtag ttcatatttc gagatttggg
      241 aaaaattcag tatctgaaga tgcaaattgg attgagagat ttggaaatta catgtttaca
      301 tttttagtaa atactttctt tggtggtaat tatactgatg cattaaatgg ctttcggatt
      361 attaagagag agttatggga cgaattgaag acagatgcac aatatttgaa tgttgaacaa
      421 caaacatgta ttagattagc taaaagaaaa attccaattt acgaaattga aagtatagag
      481 ccgaatagaa taggtggtga aagaaaaatg agaccactgc cagttggcgg catgttaagc
      541 taccaaataa ttaaagaatt cattttttgg aaataaattg gataaaaaac aaataatttt
      601 ttcattaatt ataatttgtt taatttcatt aggtttaaaa ttatacctag ttgatttttc
      661 gattccagta aattcggata atcttggtta tacactaaat gcaattgcgc acacaaatgg
      721 tgatttttca caatcatcac acagaggcat gggatggtca ttatttgtat caatgttttt
      781 tggattcatt gattcggaaa atttctttga ttattctaac acaatacgag ccctgagtat
      841 tggagtagct acattttcta tacctatgat gtatgtagta ggacgaaagt tttttgatga
      901 aagatactcg attgttctgg caagcctttt tgcatttgaa ccacatctta attataattc
      961 tggttttgga ctaacagaac cattatttca tcttgctata attggttcat tttattttat
     1021 tttaaataag aatacaagat tcgttctaat ttcgttattt ttagctggaa ttgcttattg
     1081 gataagaatc aatggggttt ttgtatttat tgtgattaca attatttact ttattacatt
     1141 aagagggtct cctaatttgt ttaggaacta tggacttggc gtagttttat ttttgttagt
     1201 aatttcacct gtattattag aacgtaatga agaatttgga gatccatttt attcagagtt
     1261 taagggtata atttttgccg ggagtcctga attgtttttc agtgagatac ataacattgt
     1321 ggctactgaa aaaacttatt ctccatttga ttatattgaa gaaaatggtt taccctcatt
     1381 tattcagact tttattttac agggatttta caacagcctt caagtagttt cgtctttggc
     1441 atttccattt ttgttcattt taataccatt tggaataata ttttcattta gagcatttga
     1501 tcaaaatcca aattatataa aggcaaactg gatcttcatt ttggtatctc ttgcgtctct
     1561 aacaatagtc atgtca
//
LOCUS       DTVV01000349            2906 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2014730, whole genome shotgun sequence.
ACCESSION   DTVV01000349 DTVV01000000
VERSION     DTVV01000349.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2014730"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="EYO86_04135"
     CDS             <1..1710
                     /locus_tag="EYO86_04135"
                     /inference="COORDINATES: protein motif:HMM:PF01578.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIC42253.1"
                     /translation="MFAYISSDFSNFNVFQNSHSSKPLLYKITGTWGNHEGSMLLWLL
                     IMSLYNVFFSFNITLEDHVRKLTIVFQSFLYLCFSLFVILTSNPFLVNSIDSNEGLGL
                     NPILQDPALAIHPPVLYIGYVGFSLILSLALAGLILNQLDNLWIQVIKRWAMFCWLML
                     TGGIALGSYWAYYELGWGGWWFWDPVENVSLMPWITGLALVHSLMITKQEQLLKRWVV
                     FLSILCFSLSILGTFLVRSGILTSVHSFAADASRGIFILILFLTITGFSFIIFILKSP
                     HSNKQINLLFINKTSALIINNIVMLIACITILLGTIYPIIIEVLTNKRMSVGAPYYNS
                     TVLPILLPGFLIMSIAPMLSWQSNKLDKIKNYIYILIVTSILTLLITYLTKFNIWGFT
                     GTILGLWIIAASLLSILHNCLKYKFKKFFIYNNALIAHMGVGIMIIGITFSSIFQTKN
                     NFTIQTGEIVNSGQYSLQLKNLKIEEKNNFQELLGIFLLHKKNEFLTTIKSSKRYYYV
                     SKVITTEAGIYHDWFQDFYLVLGNEKNNKWSITIYHNPLVSFIWIGVMIMIFSGMVGI
                     FKR"
     gene            1707..2234
                     /locus_tag="EYO86_04140"
     CDS             1707..2234
                     /locus_tag="EYO86_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIC42254.1"
                     /translation="MNKIFLFVPLILVMLISIFCLVFLLQGKDPSKPPSALLYKNLPD
                     FDIINLFDDADVISNKDLKGRIMLINFFASWCSPCKVEHPLFFEIKKKYPNILLIGID
                     YKDKQEEAINYLNTMGNPYDYVGMDNKGLIGLELGVFGLPETFIVNSKGIITYKHLGP
                     LTKKIITNEFAPFIQ"
     gene            2209..2607
                     /locus_tag="EYO86_04145"
     CDS             2209..2607
                     /locus_tag="EYO86_04145"
                     /inference="COORDINATES: protein motif:HMM:PF03918.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HIC42255.1"
                     /translation="MNLHLSSNKLVLIIFNLLILLFSTIALSDEAIDDKVKKITSNLR
                     CMTCQNQTIYDSDADFSKNIRQIVRQKLIDNQNEKEIIDFLVARYGEYIVFTPQFNRR
                     NIFLWIFPFLIFALCFVFFIFRLKKNPTKQ"
     gene            2734..>2906
                     /locus_tag="EYO86_04150"
     CDS             2734..>2906
                     /locus_tag="EYO86_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008558796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIC42256.1"
                     /translation="MLCLTTTVSAKSIRIGTEGAYPPWNNLNSAGELEGAEIDFGNEA
                     CKRMAVDCEWVTQ"
BASE COUNT          963 a          417 c          456 g         1070 t
ORIGIN      
        1 atgtttgcat acataagttc tgatttttca aactttaatg tttttcaaaa ttcacactct
       61 tccaaacctc tgttatacaa aattacagga acatggggta atcatgaagg gtcaatgttg
      121 ttatggttgc tgatcatgag cttgtacaat gtattttttt cattcaatat aaccttggaa
      181 gatcatgtgc gaaaacttac tattgttttt caatcctttt tatatttgtg tttttcttta
      241 tttgttattc ttacatctaa tcctttttta gttaattcta ttgatagtaa tgaaggtttg
      301 gggttaaacc caattctaca agatccagct ttagcaatcc atccccctgt tctatatata
      361 ggctatgttg ggttctcatt aattttaagt ttggcacttg caggcttaat attgaatcaa
      421 ttagacaatc tatggataca agtcatcaaa aggtgggcga tgttttgttg gttaatgctc
      481 actggaggaa tagctctagg gtcttattgg gcttattatg aactagggtg gggaggttgg
      541 tggttttggg atccagttga aaacgtctca ctaatgccat ggataactgg tttggcgctt
      601 gtacactcct tgatgataac gaaacaagag caattattaa aacgatgggt tgttttttta
      661 tccattttat gtttttcttt aagtattctc ggaactttct tggttcgttc gggaatttta
      721 acaagtgttc attctttcgc agctgatgca tcacgtggta tctttatttt aattctattt
      781 ttaacaatta ctggattttc atttattatt tttattctca aatctcctca ttcaaataaa
      841 caaataaatt tattattcat caacaaaaca tctgctttga tcatcaacaa tattgtaatg
      901 ttaattgcat gcatcaccat actacttggt acaatttatc ctataattat tgaagttttg
      961 acaaacaaga gaatgtcggt tggagcaccc tactataatt ccacagtact cccaatacta
     1021 cttccaggtt ttttaataat gagtattgct cctatgttat cttggcagtc taataaatta
     1081 gataagataa agaactatat atatatatta attgttacaa gtattttaac tctactcatt
     1141 acatacttaa ctaaatttaa catatggggg tttacgggaa caatattggg tttatggata
     1201 attgctgcat ctcttttgtc tattttacac aactgcctaa aatataaatt taaaaaattc
     1261 tttatctaca acaatgcgct tattgcgcat atgggtgtag gtatcatgat aataggaatt
     1321 accttttcta gtatttttca aacaaaaaat aattttacaa tacaaactgg tgaaatagta
     1381 aattcaggtc aatactcttt acaattaaaa aatttaaaaa tagaggaaaa aaataacttc
     1441 caagaactac taggtatttt tttgttgcat aaaaaaaatg aatttttaac gactattaaa
     1501 tcaagtaaaa gatattatta tgtatcaaaa gttattacta cagaagcggg aatctaccat
     1561 gattggttcc aagattttta tttagtactt ggtaacgaaa aaaacaacaa gtggtctatt
     1621 acaatttatc acaaccctct tgttagcttt atttggattg gagttatgat aatgatcttt
     1681 tcgggaatgg ttggaatttt taaaagatga acaaaatatt tttatttgtc cctttaattt
     1741 tagttatgct gatttccatt ttttgtcttg tttttttatt acaagggaag gatccgagta
     1801 agcccccaag cgcactatta tataaaaatt tgcctgattt tgacattatt aacttgtttg
     1861 atgatgcaga tgtaatttca aataaagatt taaaaggaag gattatgtta ataaattttt
     1921 ttgcttcatg gtgctctcct tgcaaggttg aacatccatt attttttgaa ataaaaaaaa
     1981 aatatccaaa catattacta ataggaattg attataaaga taaacaagaa gaagcaataa
     2041 attatttaaa cacaatgggt aatccttatg attatgttgg aatggataat aaaggattaa
     2101 taggattaga attaggtgta ttcggattgc cagaaacttt tatcgttaac tcaaaaggga
     2161 taattacgta caaacactta gggccactca ctaaaaaaat tattacaaat gaatttgcac
     2221 ctttcatcca ataaattagt tttaataatt tttaacctat taatattatt attttctact
     2281 attgctttaa gtgatgaggc aattgatgat aaagtaaaaa aaataacctc taaccttcgc
     2341 tgcatgacat gtcaaaatca aaccatttat gattccgacg cagatttttc aaaaaacata
     2401 cggcaaattg ttcgtcaaaa actaatagat aatcaaaatg agaaagaaat tattgatttt
     2461 ttagtggcgc gatatggtga atatattgtt tttacgcccc aattcaatcg gcgcaatata
     2521 ttcttgtgga tctttccctt tctcattttt gcgctatgct ttgttttttt catatttcgt
     2581 ctaaaaaaaa atccaactaa acaatagtat ttatttcatt ttttgaattt ttaattataa
     2641 caaactaact gaatttaaac tttgcgactc attttatgtg agtcattagg aggaattgtg
     2701 aaaaaattta tattaacttt actagcgcta agtatgttgt gtttaacaac tacagtaagt
     2761 gcaaaatcta tacgcattgg aacagagggg gcttatcctc catggaataa tttaaattct
     2821 gctggcgaac tagaaggggc agaaattgat tttggaaatg aagcatgcaa acgtatggct
     2881 gttgactgtg aatgggttac acagga
//
LOCUS       DTVV01000350            2022 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2021423, whole genome shotgun sequence.
ACCESSION   DTVV01000350 DTVV01000000
VERSION     DTVV01000350.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2021423"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            104..1072
                     /gene="cdd"
                     /locus_tag="EYO86_04155"
     CDS             104..1072
                     /gene="cdd"
                     /locus_tag="EYO86_04155"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:PRK09027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIC42257.1"
                     /translation="MIILKFVLKNIETVYKSLFLLFLINISFSQTKINEEVKFIISDL
                     TKVKNFNGVISSENVLKISEEYGLDNDQIMELFRPYSKIYSKAPISNYNVGAVCKGAS
                     GNLYFGSNIEILNESLSFTLHAEQAAIINAWNNGETEIRYINVGGSPCGHCLQFLNEL
                     NNADSLVIVNPKGQNFRISDLLTLAFGPKNLGVKSGLLSNQKNDLELDNDSQDKLVKR
                     ALLAAKNSYAPYSKSYSGLAVMTKDSRVFYGSYAENAAFNPSVSPVESTLSSLNLSKV
                     PFDQIVRAVLVEMKSSKVSQKNVFESILKRVAPNAELEIVYCTDKK"
     gene            1090..1872
                     /locus_tag="EYO86_04160"
     CDS             1090..1872
                     /locus_tag="EYO86_04160"
                     /inference="COORDINATES: protein motif:HMM:PF06439.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HIC42258.1"
                     /translation="MRKEFIPLLMVIVLTNCIRTNSEWKSLIVDNSLEGWHIFKDVES
                     TRGWIVENNIFIFNGVSDMESGEGDASLLSDKVYGNFEIKFDWKIVPGGNSGFMWAVN
                     EDEKYNYPYQTGQEIQILDHAIYDDPESALGGEIERNNAIEDMEARKHFVGALYDLYA
                     PTELNVAKPANEWNSYHIKIDYKANHGEVILNDVLINSFPLQGPEWDAMVQESKFSRS
                     EDYEYLGDARWYDFGKFTKGHICFQDHPGEVSFRNIMIKELD"
BASE COUNT          701 a          276 c          388 g          657 t
ORIGIN      
        1 atataaatga aaagagttta tgtacctaaa attgctgctt gcatttttat aggagtatct
       61 attattcatt taatgaactt ttttattggc taagtaattt gaaattataa ttttgaagtt
      121 tgttttaaaa aatatagaaa cggtttacaa gtctttattt ttattatttt taataaacat
      181 ttcctttagt caaactaaaa taaatgaaga ggttaaattt ataattagcg atttaactaa
      241 ggttaagaat tttaatggcg ttatcagttc tgaaaatgtt ttaaaaatat ctgaagaata
      301 tggattagat aatgatcaaa ttatggagtt attcagaccc tattctaaaa tttattctaa
      361 agctccaatt tcaaattata atgttggagc tgtatgtaaa ggagcgagtg gaaatttata
      421 ttttggatca aatattgaaa tattaaatga gtctttaagt tttacattac atgccgaaca
      481 agctgcaatt ataaatgcct ggaataatgg agaaacagaa ataagatata ttaatgttgg
      541 aggatcacct tgtggccatt gtcttcaatt tttgaatgaa ttaaataatg ccgattcttt
      601 agttattgta aatcctaaag gacagaattt tcgaataagt gatttattaa ctcttgcttt
      661 cggtccgaaa aacttagggg taaaatcggg tttgttatct aatcaaaaaa atgatttgga
      721 attagataat gactctcaag acaagttggt taaaagagct ctattagctg caaaaaatag
      781 ttatgctcca tattcaaaaa gttattcagg tcttgcagta atgacaaaag acagtagagt
      841 tttctatgga agttatgctg aaaatgcagc ttttaatcct agtgtttctc ctgtagaatc
      901 tacactctca tctttaaatc tctctaaagt accctttgat caaattgtta gagctgtatt
      961 agttgaaatg aaatcttcaa aggttagtca aaaaaatgta tttgaatcta tattaaaaag
     1021 agttgctcca aacgcagagt tagaaatagt ttattgcacc gataaaaaat agaaactttt
     1081 gatattaaga tgagaaaaga atttataccc cttttgatgg tcattgttct tacaaattgt
     1141 ataaggacga attccgaatg gaagtctttg attgtagata attctcttga gggttggcac
     1201 atttttaagg atgttgaatc aacgagggga tggattgtag aaaataacat ctttattttt
     1261 aacggggtat cagatatgga atcaggagaa ggcgatgcaa gcttgttatc ggacaaagtt
     1321 tacggcaact ttgaaataaa attcgattgg aaaatagtac ccgggggtaa tagcggtttt
     1381 atgtgggcgg taaacgaaga tgaaaaatat aattatccat atcaaacagg acaagaaatt
     1441 caaattttag atcatgcaat ttatgacgac cctgaaagtg ctctcggggg agagattgaa
     1501 cgaaataatg cgatagagga tatggaagcc cgtaagcatt ttgttggggc actatatgat
     1561 ttatatgccc ctactgaatt aaatgtagcc aagcccgcta acgaatggaa ctcctatcac
     1621 atcaaaatag attacaaagc caatcatggg gaggtaatcc tcaatgatgt tctaattaat
     1681 tcatttccac ttcaagggcc tgaatgggac gctatggttc aggagagtaa attcagtaga
     1741 tcggaagatt atgaatattt gggcgatgca cggtggtatg attttggaaa atttaccaag
     1801 ggtcatattt gttttcagga tcatccaggt gaggtctcct tcagaaatat tatgattaaa
     1861 gagcttgatt gaatttgttt tttaaatggc cgagctaata cttactaaat aaaagccaac
     1921 agattaccag ctttctcaac cacttctcca atcacaacat cgtaagtgac gggggacggt
     1981 cgggatgaaa gaatgaaacg cctctggctc aactagcccc gc
//
LOCUS       DTVV01000351            1131 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2022459, whole genome shotgun sequence.
ACCESSION   DTVV01000351 DTVV01000000
VERSION     DTVV01000351.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2022459"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="EYO86_04165"
     CDS             <1..1084
                     /locus_tag="EYO86_04165"
                     /inference="COORDINATES: protein motif:HMM:PF00521.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIC42259.1"
                     /translation="DGCIAYIDNKEITIEELTKFVKGPDFPTGGIIKGRSGILSAFKT
                     GRGSIVIESKVQIEEKSKDKKKSIIVTEIPYTINKSKLVEKIAEVTKNKIVEDISDIR
                     DESNREGVRIVVEIKKGANPDIILANLFKHTPLRTSFGVNALALKDGTSPEVMDLKKM
                     ISIFVSFREEVTRKRFFFELSKARERVHNLTGLMIAVNNLDLVIKLIRNSKNPNDAKI
                     SLLKKEWPAKDLISFIKIVDDPRYKLKKAGKYDLSPEQADAILDLRLQKLTGLERDKI
                     VEQAKELGKKINKYLNLLQKKELLVHEIKKELIEIKENFSDERRTIIGTETADIEEED
                     LIESEDMVITVTHRGIWRIGACFGRI"
BASE COUNT          476 a          112 c          212 g          331 t
ORIGIN      
        1 agatggatgt attgcatata tagataataa agaaattaca attgaggaat taacaaaatt
       61 tgtaaaaggt cctgattttc caactggtgg aataataaaa ggtagaagtg gaatactatc
      121 agcatttaag actggaaggg ggtcaattgt catagaatct aaagttcaaa tagaagagaa
      181 atcaaaggat aaaaagaaat ctattattgt tactgaaatt ccatatacga ttaataaatc
      241 taaattagtt gagaagattg ccgaagttac aaaaaacaaa atcgttgaag atatttctga
      301 tataagagat gaatccaacc gtgaaggagt aagaattgtt gttgaaatta agaaaggtgc
      361 aaatccagat attattttag caaatttatt taaacataca ccattaagaa caagttttgg
      421 tgttaatgca ttagcactga aagatggcac aagtcctgag gttatggatt tgaaaaaaat
      481 gatcagtatt tttgtatctt ttagagaaga agtaacaaga aaaagatttt ttttcgaatt
      541 atcaaaagca agagaaagag ttcacaattt aacaggttta atgattgctg ttaataattt
      601 agatttagta attaaattaa taagaaattc aaaaaatcca aatgatgcaa aaataagtct
      661 tttaaaaaaa gagtggcctg caaaagattt aattagtttc attaaaattg ttgatgatcc
      721 aagatataaa ttaaaaaaag ctggaaaata tgatttatcc cctgaacaag ctgatgcaat
      781 tttagattta agacttcaaa aattaaccgg tttggaaaga gataagattg tagaacaagc
      841 aaaagaatta ggaaaaaaaa ttaacaaata tttaaatttg ttacaaaaaa aagaattgct
      901 tgttcatgaa attaaaaaag aacttataga aataaaagaa aatttttctg atgaaagaag
      961 aacaattatt ggtactgaaa cagctgacat agaagaagag gatcttattg aatcagagga
     1021 tatggttatt actgtaactc acagaggaat ttggagaatt ggcgcatgct ttgggcgaat
     1081 ttgagcgtgg cgaagtcgat ttggagaacc ttcaggaaga attttcggat g
//
LOCUS       DTVV01000352            1288 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2023606, whole genome shotgun sequence.
ACCESSION   DTVV01000352 DTVV01000000
VERSION     DTVV01000352.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2023606"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="EYO86_04170"
     CDS             <1..167
                     /locus_tag="EYO86_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein"
                     /protein_id="HIC42260.1"
                     /translation="IVNKGIAILFVEQNVKLALNMADRGYVLESGHLVIEGKSDYLLS
                     SEKLKKVFLG"
     gene            complement(171..956)
                     /locus_tag="EYO86_04175"
     CDS             complement(171..956)
                     /locus_tag="EYO86_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HIC42261.1"
                     /translation="MLNIQDPTEARKIIRNNLYDKQTAGTANQYVQGNVCILPSKHSI
                     HFETFCQKNPKPCPLIGLGYKGDPNLRDLGDIDIRTDVPKYRIWEKGEIVDEPLDIKN
                     YWNEDLVTFILGCSMSFELPLIEAGIEIQHIKNNTTVPMYRTSIECEPAGQFHGKMVV
                     SMRPLSTKDAIKSIQICSKFPSVHGAPIHFGNPEEIGVKDIMNPEYGDPPRPLKNNEI
                     PVFWACGVTPQLILEKSKLDFCITHKPGCMLITDKLNSDLATL"
BASE COUNT          429 a          216 c          199 g          444 t
ORIGIN      
        1 cgattgtaaa taaaggaatt gcaattttat ttgtagaaca aaatgtaaaa ctagccttaa
       61 acatggctga tcgaggatat gtccttgaaa gtggccatct agttatagaa ggaaaatcag
      121 attatctatt aagcagtgaa aaacttaaaa aggttttttt aggataataa ttacaaggtt
      181 gctaaatctg aatttaattt atcagtaatt aacatacatc ctggtttatg agtaatacaa
      241 aaatctaatt ttgatttttc taaaattaat tgcggtgtaa ctccgcaagc ccagaaaact
      301 ggtatttcat tatttttcaa aggccttggt ggatcaccat attctggatt cataatatct
      361 ttcaccccaa tttcttcagg attgccaaag tgaattggag caccgtgaac tgaaggaaat
      421 ttagagcaaa tttgaattga tttaatcgca tctttagtgg aaagtggcct catggaaacc
      481 accattttcc catgaaattg tcctgcgggc tcacattcaa ttgaagttct atacattggc
      541 acggtagtat tattttttat atgttgaatt tcaattcctg cttctattaa aggtaactca
      601 aacgacattg aacatcctaa aataaaagtt actaaatctt cattccagta attttttata
      661 tctaaaggct catctacaat ctcgcctttc tcccaaattc tgtattttgg gacatctgtt
      721 ctaatatcaa tgtcacctaa atctcttaaa ttaggatccc ctttatatcc aagtccaatt
      781 agtgggcacg gtttaggatt cttctgacaa aaagtttcaa agtgtattga gtgtttgctt
      841 ggtaaaatgc atacgttacc ttgtacatac tgatttgctg taccagctgt ttgtttatca
      901 tataaattat ttctaattat ttttctagct tcggttggat cttgtatatt taacattaat
      961 tatattctac caccaactac gaggaatgtt gaggaattag ttgcttgttt ttagtgatta
     1021 gcttcctaca aaaagaaaag taaccatcca gataaaaaca aaaataatag caaataccca
     1081 tgggtgaatt ttaatatagt tataagttgc cttacccaat ccaaatgaca ttaaagttcc
     1141 gacaacaata gcaaagaccg aaagaagtat aaaaaatact tttatcgtta tcattggctc
     1201 accctaccac caactacgag gaatgttgag ttgatagtta tttaaattta tcacaaatca
     1261 acattgtagc gcctaagtct ttttttta
//
LOCUS       DTVV01000353            1398 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2024687, whole genome shotgun sequence.
ACCESSION   DTVV01000353 DTVV01000000
VERSION     DTVV01000353.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2024687"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="rpsC"
                     /locus_tag="EYO86_04180"
     CDS             complement(<1..574)
                     /gene="rpsC"
                     /locus_tag="EYO86_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIC42262.1"
                     /translation="MGQKVNPNGIRLGINKTWSSRWFSKKNYTKLLHEDLNIKKYVEA
                     KLKSASISKIIIERAAKKLRLTIFSSRPGIIIGKKGSDIENLKNVLTKMSNLQVFIDI
                     KEVRKPEVDAKLVAENIAVQLEKRISFRRAMKKAVQSSMRLGAKGIKVVCSGRLGGSE
                     IARTEKYHEGSVPLHTLRSDIDYATAKAETT"
     gene            complement(579..953)
                     /locus_tag="EYO86_04185"
     CDS             complement(579..953)
                     /locus_tag="EYO86_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIC42263.1"
                     /translation="MDNKMIAIAKDNTIRVSSLKLANVVRSIVNLKASKAVNQLKFSQ
                     KRISLNVLKVLNAAIANAENNKQLDIDNLFVKEAYVGKSLHMKRFRPRAKGRASSIIK
                     PFSKLTVIVEERKEISNKEESK"
     gene            complement(957..1235)
                     /gene="rpsS"
                     /locus_tag="EYO86_04190"
     CDS             complement(957..1235)
                     /gene="rpsS"
                     /locus_tag="EYO86_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIC42264.1"
                     /translation="MARSVWKGPFVDITLIKKAEKVSESGRKEVIKTWSRRSTILPQF
                     VGLTFGVYNGKKFIPVSVSENMVGHKLGEFSPTRTFKGHTTADKKVSK"
     gene            complement(1235..>1398)
                     /gene="rplB"
                     /locus_tag="EYO86_04195"
     CDS             complement(1235..>1398)
                     /gene="rplB"
                     /locus_tag="EYO86_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565936.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIC42265.1"
                     /translation="GVVMNPVDHPHGGGEGRTSGGRHPVTPWGKATKGKKTRTNKKTN
                     VFILKRRKK"
BASE COUNT          373 a          271 c          197 g          557 t
ORIGIN      
        1 acgttgtttc agcctttgca gttgcatagt ctatatcact ccttaaggta tgaagaggga
       61 cactaccttc atgatatttt tcagttcttg caatttctga tccacccaat cttccgctac
      121 agactacttt gattcctttt gcacctaaac gcattgagga ttgtacagct tttttcatag
      181 ctcttctaaa tgatattcgt ttctcaagtt gaacagcaat attttcagct actaattttg
      241 catcgacttc aggtttacgc acttccttaa tatctataaa aacctgtaaa tttgacattt
      301 ttgtaagtac gttttttaga ttttcaatat cagagccttt ttttccaatt attatacctg
      361 gccttgaact aaaaattgtt aatcgtaatt tcttagcagc tctttcaatt ataatctttg
      421 aaatgcttgc ggatttaagc tttgcttcca cgtatttttt tatatttaaa tcttcatgta
      481 aaagttttgt ataatttttt ttggaaaacc atcgtgaaga ccatgtttta ttgataccaa
      541 gtcttatacc gtttggattt actttttgtc ccataaaatt atttactttc ttctttgttt
      601 gatatttctt ttctctcttc aactataact gtaagtttac taaatggttt aatgattgat
      661 gatgccctac ccttagctcg cggcctaaat ctcttcatgt gtaaactttt accaacataa
      721 gcttctttga caaacaaatt atcaatatca agttgtttat tgttttcggc atttgctatt
      781 gctgcattca aaacttttag aacatttaat gatattcttt tttgagaaaa ttttaattga
      841 ttaacagctt tgcttgcttt taaattaaca attgaacgta caacatttgc taatttaaga
      901 gagctaactc ttatagtatt gtctttggcg atagctatca ttttattatc cataaattat
      961 ttgcttactt ttttgtcagc tgtagtgtga cctttaaatg tacgagttgg tgaaaactcc
     1021 cctagcttgt ggcccaccat attctcactg actgaaactg gaataaattt ttttccatta
     1081 taaacaccaa atgttaatcc aacaaactga gggagtatgg tcgatcgacg cgaccatgtt
     1141 ttaattactt ctttacgacc agattctgat accttctctg ccttcttaat cagagtaata
     1201 tctacaaaag gtcccttcca aactgatcta gccattattt tttccttctt ttaagtataa
     1261 acacatttgt ttttttgtta gtgcgcgttt tttttccttt tgttgctttt ccccaaggag
     1321 ttacagggtg acgtccgcca gaagttcttc cttcacctcc accatgaggg tggtcgactg
     1381 gattcattac aacacctc
//
LOCUS       DTVV01000354            1520 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2033373, whole genome shotgun sequence.
ACCESSION   DTVV01000354 DTVV01000000
VERSION     DTVV01000354.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2033373"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1520
                     /locus_tag="EYO86_04200"
     CDS             <1..>1520
                     /locus_tag="EYO86_04200"
                     /inference="COORDINATES: protein motif:HMM:PF00873.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIC42266.1"
                     /translation="KLDIIKSCLKRPIIIYSLIILILFSGLLALFKIPIQLTPDVRKP
                     VIEITTSWQGGSPSEVEREIVVKQEDVLKSVKGVERIRSNAYDKKSEIKLEFSSTKNF
                     QTSLLMTSNALDRVRGMPEEIMKPIIETSGSEDSPIAWIMLRPIKKINKSMSEFGDLV
                     DNLVKTELEKIPGISRTNIFGGSKTEMQVIVDPQRLSLFKLTISDIANSLISSNISLT
                     AGDVDEGKRKYLIRAEGQLDSPEKIKKVVIKVNKDEQTQFSSKIFLEDVAEVKYGIKE
                     PVAYIRSLGREVIAINLVRDVGVNVLETMKEVKETIKRLNPLLNKEGLELKQVYDETI
                     YINSSIDLVQQNIIVGGLLAALILLLFLNSARATLVIATTIPLTIIGTFVAMAILGKS
                     LNVISLAGLAFAVGMVIDAAIVVLENIFRHKEMGKSDYDASLIGTKEVSQAVFLSALT
                     TVLVFIPLLIVQVEAGQLFRDISVAICVAISLSLVLAVTLVPVISNRLKLLQTDNK"
BASE COUNT          579 a          185 c          263 g          493 t
ORIGIN      
        1 ataaattgga tattattaaa agttgtctta aaagaccaat aataatttat tctctgataa
       61 tattaattct tttttctgga cttttagcat tatttaaaat tccaattcaa ttaacacctg
      121 atgtaagaaa acctgtaatt gagataacca caagttggca aggcggttcc cctagtgaag
      181 tagagagaga aattgttgtt aagcaagaag atgttttaaa atccgtaaaa ggagtggaaa
      241 gaataagaag taatgcttat gacaaaaaat cagaaataaa attagagttt agttctacaa
      301 aaaattttca aacatcttta ttaatgacat caaatgcact tgatagagta agaggtatgc
      361 ctgaagaaat catgaaacca attatagaaa cctctggaag tgaagattca cctattgcat
      421 ggattatgct aagacctata aaaaaaatta ataaatctat gtcagagttt ggggatttag
      481 tagataattt agttaaaaca gaattagaaa aaattccagg catttcaagg actaatattt
      541 ttggaggcag caaaactgaa atgcaagtta ttgtagatcc tcaaagatta tctttgttca
      601 aattaacaat ttcagatatt gcaaatagtt taatttcatc caatatttct ctaaccgcag
      661 gtgatgtgga tgaaggaaaa agaaaatatt taataagagc tgaaggtcaa ttagatagtc
      721 ctgaaaaaat aaaaaaagtt gttataaaag taaacaaaga cgaacaaact caattttcat
      781 caaagatttt tttagaagat gtggccgaag ttaaatacgg aattaaagaa cctgtcgcat
      841 atattcgatc attaggaaga gaggtaattg ctataaattt agtaagagat gtaggggtaa
      901 atgttttaga aacaatgaaa gaagtaaaag aaacaataaa aagattaaat cccctattaa
      961 ataaagaagg ccttgaactt aaacaagttt atgacgaaac tatttacatc aattcttcaa
     1021 tagatttagt tcaacaaaat ataattgttg ggggtttgtt ggctgcacta atattattat
     1081 tatttctaaa ctcagctaga gctacactgg taattgcaac tactatacct cttacaataa
     1141 ttggaacttt tgttgcaatg gctatattag gaaaatcctt aaatgtaata agtttagctg
     1201 gattagcttt tgctgttgga atggttattg atgctgcgat tgttgtttta gaaaatattt
     1261 ttagacataa agaaatggga aaaagtgatt atgatgcatc tttaattggg acaaaagaag
     1321 tgtcgcaagc agtttttttg tctgcattaa caactgtttt agttttcatt ccgttactaa
     1381 ttgtacaggt agaagctggt caattattta gagatatatc tgttgcaatt tgcgttgcta
     1441 taagcttatc gctagtctta gctgtaacat tagtcccggt tatttctaat agattaaaac
     1501 ttttacaaac tgataataaa
//
LOCUS       DTVV01000355            1318 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2035436, whole genome shotgun sequence.
ACCESSION   DTVV01000355 DTVV01000000
VERSION     DTVV01000355.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2035436"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(142..>1318)
                     /locus_tag="EYO86_04205"
     CDS             complement(142..>1318)
                     /locus_tag="EYO86_04205"
                     /inference="COORDINATES: protein motif:HMM:PF16327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmF"
                     /protein_id="HIC42267.1"
                     /translation="ALVHSLVTVEKKMMLQKWVLLLAILAFLLSVVGTFLVRSGILTS
                     VHTFALDPARGIYILVFIGILGAYSLILFALNSKKFLDKNYISFLSREGSILVNNILM
                     IVVCSTVFLGTIYPLIIEAFSGNKISVGEPYYNSTVVPIIVPAILVMGIGPLLSWKKV
                     NIIKILKKTLPSIVLTIIVTTIFLWIYQSYNILGLVGIILSCWIISNIVFTLFAQLKV
                     KGKINKFTQDNIPKFSKSMVMAHLGIALLILGVTGSSVWQQEKILRMKVNDETTIHNY
                     NIIFKQIKEVVEKNYIALQGNFWIYNKKNNIITKLNPENRFYPVTNNSTTEASIHTNL
                     LRDLYIVLGDGNEKDGWVVRVYYNPLVVWIWIGAFVIFLGGLFSINTNIKILKRLSL"
BASE COUNT          494 a          220 c          171 g          433 t
ORIGIN      
        1 aatccgtttc aaaagtcggt acttttttat ctattaggac agatggaagt tctgaaggat
       61 cacgttctat gactaacaaa taaaaaaaaa ccaacaacac gaaaaagaac gcaataaatg
      121 gaaaaattaa tatttttttt ctcatagtga caaacgtttt aaaattttta tattagtatt
      181 tatcgaaaac aatcctccta aaaatataac aaaagcacca atccatatcc aaactacaag
      241 ggggttataa tataccctaa caacccaacc gtctttttca ttgccatcac ctaatacgat
      301 atacaaatcc cttaagagat tggtgtgtat cgaagcctct gttgttgagt tattagtcac
      361 aggataaaac cgattttctg gatttaattt ggttataata ttattcttct tattataaat
      421 ccaaaaattt ccttgtagcg caatatagtt tttttccaca acttctttta tttgtttaaa
      481 gataatatta taattatgaa tagtagtttc atcgttaacc ttcattcgaa gaattttctc
      541 ttgctgccat acacttgatc cagtaacacc caatattaac aaagcaatac caagatgagc
      601 catcaccata ctcttagaaa atttagggat attatcttgt gtaaatttat ttattttacc
      661 ctttactttt aattgtgcaa ataaagtaaa aacgatatta gatattatcc aacaagatag
      721 aataatgcct actagtccaa gtatgttata tgattgatat atccaaagaa atatggtggt
      781 gactataata gtaagcacga tactaggaag agttttcttt aaaattttta taatattaac
      841 ttttttccat gataataatg gtccaatccc cataactaaa attgcaggaa caattatcgg
      901 gacaactgtt gaattataat agggttcacc tacagatatt ttgttaccag aaaaagcttc
      961 gattattaaa ggatatattg ttcctaaaaa tacagtagaa cacactacta tcataagtat
     1021 attatttact aatattgagc cttctctact caggaaagaa atataatttt tgtctaaaaa
     1081 tttttttgaa tttagtgcaa atagaatcaa cgaatacgct ccaagtattc caataaatac
     1141 aagaatgtat atccctcttg caggatccag agcaaaagta tgaacgctag tcagtattcc
     1201 tgatctaaca agaaatgttc caacaacact taacaaaaaa gcaagtattg ccaaaagcaa
     1261 aacccatttt tgaagcatca tttttttttc aacagtgaca agagaatgaa ccaaagct
//
LOCUS       DTVV01000356            2262 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2035923, whole genome shotgun sequence.
ACCESSION   DTVV01000356 DTVV01000000
VERSION     DTVV01000356.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2035923"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /gene="carB"
                     /locus_tag="EYO86_04210"
     CDS             <1..1738
                     /gene="carB"
                     /locus_tag="EYO86_04210"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIC42268.1"
                     /translation="LKKLQESEIRELRTKYKIKPVYRLVDTCAGEFNSKTPYLYSTYD
                     WCFENEKYCEADPTDKNKIIILGSGPNRIGQGIEFDYCCVHAVFALKEKNIETIMINC
                     NPETVSTDYDTSDRLYFEPLTAESVIEIHNKEAENGNVLGVYVQFGGQTPLKIAKQLE
                     EAGIKILGTSTQAIDLAEDRDLFSNVLNKLNIKQPKNGTSNNIKDTLQIANQIKYPVL
                     IRPSYVLGGRAMEIAYDDKALKSFAAKAMSVSTNNTILIDQYLENAIEIDVDLIRDSG
                     GSVFIAGIMEHIEEAGIHSGDSACSLPPFSIDKNIENQIVTWVTNIANEINVIGLMNT
                     QLAIKDNQLYVLEVNPRASRTVPFVAKSIGIPIAKIASKVMVGDRLLDLLPSLDRINL
                     KTFNVKESVFPFNKFDGVDLILGPEMKSTGEVMGIDNNFLSAYIKSQVASGTFLPKKG
                     KVFLSIDNDSKEKIIAIAKKLKNLKFNIVATRGTASYLSNHNIDVEIIKKVKEGSPHI
                     VDSLLSNDINLVINTTKTQGSIRDSYSIRRTSLMNNIPYYTTIAGAKVAVDAIESLKN
                     HALTVQSIQSIH"
     gene            1779..2252
                     /gene="greA"
                     /locus_tag="EYO86_04215"
     CDS             1779..2252
                     /gene="greA"
                     /locus_tag="EYO86_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008040312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIC42269.1"
                     /translation="MNKIPMTTSGYEHLQEELKKLVNEDRPNIIAAIAEARSHGDLSE
                     NAEYQYAKEQQSLIEGKIQELESVTALAEIIDVSTLSGEEILFGAIVEIKDDESGDVS
                     TYQIVGEYESDIENKKLSISSPLAKGLIGKTKDDVVEIKSPKGIKSYMILSVKFK"
BASE COUNT          863 a          333 c          402 g          664 t
ORIGIN      
        1 actaaaaaaa ttgcaggaaa gtgaaattcg tgaattacgc actaaataca aaataaaacc
       61 tgtatatcga ctagttgaca cttgtgctgg tgaatttaat tctaaaacgc cctatttata
      121 ttcaacttat gattggtgtt ttgaaaatga aaaatactgc gaagctgacc ctactgacaa
      181 gaataaaatt attattttag ggagtgggcc aaacaggata ggccaaggga ttgaatttga
      241 ctattgttgt gtacatgctg tttttgctct taaagaaaaa aatattgaaa cgattatgat
      301 caattgcaac cctgaaactg tgtcaacaga ttatgataca tctgatagat tatactttga
      361 gccgttaact gcagagagtg taattgaaat tcataacaaa gaagcagaaa atggcaatgt
      421 attgggagtt tatgtgcagt ttggaggtca gacacctctc aaaattgcta aacaattaga
      481 agaagctgga ataaaaattt taggaacttc aacccaagct attgatcttg cagaggatag
      541 agatttattt agtaatgttc ttaataaact taacatcaag caacctaaaa acggtacaag
      601 taacaatatc aaagacaccc ttcaaatagc taatcaaatt aaatacccag ttctcatacg
      661 cccctcctat gtcctgggag gaagagcaat ggaaattgcc tatgatgata aagctttaaa
      721 atcatttgct gcaaaagcaa tgagtgtttc aaccaataat actattctta ttgatcaata
      781 tttagaaaat gctatcgaaa ttgacgttga tctaattcgt gacagtggag ggtctgtttt
      841 tattgccggc attatggaac atattgaaga ggcaggtatc cattctgggg atagcgcctg
      901 ttctcttcct cctttttcaa ttgataaaaa tatagaaaat caaattgtaa catgggtaac
      961 taacattgct aatgaaatta atgtcatagg tttaatgaat acccaactag caatcaaaga
     1021 taatcaatta tacgttttag aagtaaaccc tagagcaagc agaacggtgc catttgttgc
     1081 aaaatcaata ggcataccta tcgcaaaaat agcttcaaaa gttatggttg gtgatcgcct
     1141 gctggattta ttaccttcgc tagatagaat aaatttgaaa acattcaatg taaaggagtc
     1201 tgtttttcca tttaataaat ttgatggcgt agatttaata cttggaccag aaatgaaatc
     1261 aacaggggaa gttatgggta tagataataa ttttttatca gcatatatta aaagccaagt
     1321 tgcctctggt actttcttac ctaaaaaagg aaaggtattt ttgtctattg ataatgatag
     1381 caaagaaaaa attattgcca ttgccaaaaa actaaaaaat cttaaattta atatagtagc
     1441 gactcggggc accgcaagct accttagcaa tcataatatt gatgtagaga taataaaaaa
     1501 agttaaagaa ggcagcccgc atattgtaga ttctttacta tctaacgata ttaacttagt
     1561 tattaatacc acaaaaacac agggctcaat cagagatagt tatagtatta gaagaacttc
     1621 gttaatgaat aacattccat attacacgac catcgcagga gcaaaagtag ctgtagatgc
     1681 aatagaaagc ttaaaaaatc acgctcttac agtacaaagc attcagtcaa ttcattgaaa
     1741 tttattgact ttaaaatata atagatcatc ataatattat gaataaaata ccaatgacaa
     1801 cctcaggtta tgagcatttg caagaagaat taaagaagct tgtaaacgaa gatagaccta
     1861 atatcattgc tgcaattgca gaagcaagaa gtcatgggga tttatctgaa aatgcagaat
     1921 atcaatatgc taaagaacag caaagtttaa ttgaaggcaa aatacaagaa ttagaatcag
     1981 taacagcttt ggcagagatt attgatgtat caactttatc aggcgaagaa atactttttg
     2041 gtgcaattgt tgaaataaaa gatgatgagt ctggtgatgt gtcaacatat caaattgtag
     2101 gagaatatga atctgatatt gaaaataaaa aactttcaat tagtagtcct ctcgcaaaag
     2161 gattaattgg caaaacaaaa gatgatgttg tagaaatcaa aagccctaag ggaattaaat
     2221 cctacatgat attgtctgtt aaatttaaat aagaaattgg aa
//
LOCUS       DTVV01000357            1802 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2035924, whole genome shotgun sequence.
ACCESSION   DTVV01000357 DTVV01000000
VERSION     DTVV01000357.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2035924"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            410..694
                     /locus_tag="EYO86_04220"
     CDS             410..694
                     /locus_tag="EYO86_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42270.1"
                     /translation="MTVCSPPFEDPSCHALNKFDASILETNTWHDFIIETRQSLKTDG
                     YVKIFQNKNLIFKKSSLKTFYKYNGGLRFWIGPYIWSGAHIAKDEPNHEF"
     gene            766..1062
                     /locus_tag="EYO86_04225"
     CDS             766..1062
                     /locus_tag="EYO86_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42271.1"
                     /translation="MKNKFLIGLTIVILLIILRFCFPSKYTSNDELNDLQKLVNDEKV
                     IKKIENICLGKGFKNYTSENMGLKKMLQQQRFNECKVGELCKEVEKLMDHKICN"
     gene            1319..1582
                     /locus_tag="EYO86_04230"
     CDS             1319..1582
                     /locus_tag="EYO86_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42272.1"
                     /translation="MKNFTISYQVNFTYEDPSENISRLIDITMQSKNLHSLQKILHEH
                     SIEDDVERNENAKSKVIDINSEYFLIVDHKGKQVWKDWNFKKI"
BASE COUNT          707 a          295 c          256 g          544 t
ORIGIN      
        1 aagtgggatg caacttcaac caatcacatt aaagaatcaa tcattagaga cttagaattt
       61 aaaagagata cttcttcaaa agaaagtatt cattatattt tagagacaca aaaatgtgtt
      121 cctcattcca ctagtggaga ttgtgagaga ggtattagaa gatcacaatt aagaaatcat
      181 aaaaactata aacgaaataa agatttgata tttaatttca gtatttatca aaaaaaatct
      241 aatacgcctg gccatagtgg gaatcatcaa tcctcagaaa taaataaatc aaaaggcgga
      301 ggatcttgga gaatatattt gaaataaaac ctaaatttga tggtaaagtt ccaaccaatc
      361 ccacacttgt aatctattta gatcctaata aaaaaaatta catatcatta tgacagtttg
      421 ttcccctcca tttgaggatc caagctgtca tgctcttaac aaatttgatg caagtatcct
      481 ggagaccaat acctggcatg attttatcat tgaaacacgc caatcactca aaactgatgg
      541 ttatgtaaaa atatttcaaa ataaaaattt aatatttaaa aaaagtagtt taaaaacttt
      601 ctataaatac aatggaggtc ttcggttttg gataggacct tacatatgga gtggggcaca
      661 catagcaaaa gatgaaccta atcatgaatt ctagtatgat aatatcaacg ttcagattaa
      721 ataatttaat ctcaacattc cccatggttg gaggtaggat ggatcgtgaa gaataaattc
      781 ttaattggtt taacaatagt tattttatta ataatattaa gattttgctt ccctagtaaa
      841 tatacatcta atgatgaact aaatgatctc caaaagttag taaatgatga aaaagttatt
      901 aaaaaaatag aaaacatttg ccttgggaaa ggatttaaaa attacacttc ggaaaatatg
      961 ggtttaaaaa aaatgcttca acaacaaagg ttcaatgagt gcaaggttgg tgagttgtgt
     1021 aaagaagttg aaaaattaat ggatcataaa atttgtaatt gaattttatg gaatcacatt
     1081 tgattcatta gttctttctc acaacaccta cacaacaatt agcttaaaaa aaattaatca
     1141 aatttcttga tactctcccg acactctcac ggatgagata ttgagtgctt gctcaaaaga
     1201 acaaccacct aatcacgacg atcacgccaa ctaaaaacct cccctttccc ccgcttattt
     1261 tcccccttaa tcccgtatcg atatcgtggt aaaagattaa tatcaaataa ctaaaattat
     1321 gaaaaatttt accatatctt atcaagtaaa ctttacctat gaagatccta gtgaaaatat
     1381 ttcaagatta attgatataa caatgcaatc aaagaacttg cattctttac aaaaaatttt
     1441 acatgaacat tctatagaag atgatgttga aagaaatgaa aatgcaaaaa gtaaagttat
     1501 cgacataaat tctgaatatt ttcttattgt tgatcataaa gggaaacaag tttggaaaga
     1561 ttggaatttt aaaaaaatat aaataattaa ctccttatat aagaaaatta acttaaataa
     1621 ccctaagtcc tccctacaaa gaacaaccac atcaaaacaa cgatcacgcc aatcaccaaa
     1681 gcgtcgtgga taagatccaa caaatcatgt taccccccct ttccttaagt ttcctaacca
     1741 agtgcttggc ttgtatgtga agatatcttt gtaatggttt caacgttttc caaccccctt
     1801 gc
//
LOCUS       DTVV01000358            1041 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2040644, whole genome shotgun sequence.
ACCESSION   DTVV01000358 DTVV01000000
VERSION     DTVV01000358.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2040644"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            78..371
                     /locus_tag="EYO86_04235"
     CDS             78..371
                     /locus_tag="EYO86_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIC42273.1"
                     /translation="MTTCDEAKKMRTGITIEAKIRDKGEPRTVNTKFGETQVCDAYLE
                     DDSGSIKFTIWGDDIQKVKNGDQVKIENGYTTEFRGEVQLNKSRNGKMDVTPS"
     gene            complement(396..851)
                     /gene="msrA"
                     /locus_tag="EYO86_04240"
     CDS             complement(396..851)
                     /gene="msrA"
                     /locus_tag="EYO86_04240"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIC42274.1"
                     /translation="MKATFAAGCFWHVEEIFRKTSGVKSTQVGYSDGVTKNPTYEDVC
                     TDTTGHAEAVEVDYDPQEVSYEELLKIFWNNHNPTTLNRQGPDVGKQYRSAVFFHTPE
                     QKKAAIEMKEKLNPIAREKFQSDIVTEIKPASDFYRAEEYHQQYFSKSS"
BASE COUNT          357 a          175 c          202 g          307 t
ORIGIN      
        1 gcttcgaaac ccctgcactt taaatggatt ttgtacattt aaaaaaagtt attaattgaa
       61 gaagttttga tttttgtatg acaacatgtg atgaagcaaa aaaaatgaga accgggataa
      121 caattgaagc aaaaatcaga gacaaagggg aaccaaggac tgtaaataca aaatttggag
      181 aaacccaagt ctgtgatgca tatctagagg atgattctgg aagcatcaag ttcacaattt
      241 ggggcgatga tattcaaaaa gtgaagaacg gggatcaggt caaaatagaa aatggatata
      301 ccacagaatt caggggagaa gtccagctga acaaatccag aaatggaaaa atggacgtaa
      361 ctcctagcta atatcaaaaa tagaaataat agattttagg aggacttgga aaaatactgc
      421 tggtgatatt cttcagccct atagaaatct gatgcgggct taatctctgt gactatatca
      481 ctctgaaatt tttctcttgc tattgggtta agtttttctt tcatttctat tgctgctttt
      541 ttctgttctg gcgtatgaaa aaatactgca gatctatact gcttgcctac atcaggtccc
      601 tgtctgttca gtgtagttgg attatgattg ttccaaaaaa tctttagtaa ttcttcatac
      661 gaaacttctt ggggatcgta atcaacctcg acagcttcag catgtccagt agtgtctgta
      721 cacacatctt cgtaagtagg gttttttgtt actccatcac tgtaaccaac ttgtgttgat
      781 ttgacaccac tggtttttct aaatatctcc tcaacgtgcc aaaagcaacc tgccgcaaat
      841 gtagccttca tactatatta atagtaaatt atctaattaa tattctagca tggaaacggc
      901 cgacattgaa gctataccta tcataaagac atttgaccta aaagatgaaa aggacgccta
      961 tgatgcagct gaagaaatgg tgagaattgg gttttacaaa gaaaaaaaag gattcaaagt
     1021 tttaatgcca agggaatcaa a
//
LOCUS       DTVV01000359            2797 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2042607, whole genome shotgun sequence.
ACCESSION   DTVV01000359 DTVV01000000
VERSION     DTVV01000359.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2042607"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="EYO86_04245"
     CDS             complement(<1..937)
                     /locus_tag="EYO86_04245"
                     /inference="COORDINATES: protein motif:HMM:PF00208.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HIC42275.1"
                     /translation="MLKQDKKPSFLENVNMMVNDTLNHIDIDPNIAKILKTCRSVLQV
                     KFPIKLKGEIKIFHGWRAVHSNHRLPVKGGMRFSTNVNQEEVEALASLMTFKCAVVDV
                     PFGGAKGGLLIDPQKYDEESLEKITKKFARELIRRGYLSPARDVPAPDVGTSQREMGW
                     ILDAYKSLRPDDINHMACVTGKSVDHGGIKGRLEATGRGVCEALKEFFRHAAEVQRAG
                     VNKELSDQNIIIQGFGNVGFNSAKALFQNGAKIIGIAEKDGGIFNDKGIDIKELEKYQ
                     LENNTILNFPTTKSIKNSADLLSYDCDILIPAALEN"
     gene            1047..1502
                     /locus_tag="EYO86_04250"
     CDS             1047..1502
                     /locus_tag="EYO86_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIC42276.1"
                     /translation="MVNVMSFKSRLGWISAVEKNNQIISVSFGKSKTKGSSTELKKLQ
                     KILSNYFSGKTKKITSNILMEGTKLQIKIWKELQKIPYGQTKSYGEIAKIVKTSPRYV
                     GNVCGQNNHLLLVPCHRVVRSDGNLGGFSGLGGLTLKKNLLDLEQSIDE"
     gene            1521..2237
                     /locus_tag="EYO86_04255"
     CDS             1521..2237
                     /locus_tag="EYO86_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIC42277.1"
                     /translation="MKIIILQHIACETPGYIKDLMIKDGLDLTTIELDEGETIPDDIK
                     QFDGMFCMGGPMDTWMEKDYPWLVEEKKKIKEFVVELEKPFLGFCLGCQLLGEVVGGQ
                     VVKSSPPEIGILDIQMGEEKKEDHLFSRFPNSIKALQWHSYEVAGLERNNDVTLLGSS
                     PLTKYQIFKYKDHAYGIQFHIEIKDNTVSNWGCVPEYKNALEESLGKGALNKFETIAA
                     QNMKDMNNLATIVYKNFLDI"
     gene            2239..>2797
                     /locus_tag="EYO86_04260"
     CDS             2239..>2797
                     /locus_tag="EYO86_04260"
                     /inference="COORDINATES: protein motif:HMM:PF00596.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HIC42278.1"
                     /translation="MKNKWNSIEEKKYIKKYKNNHIPQDLALRIYTTHLLGREKTLVL
                     HGGGNTSLKTTSKNIFNKKIDIMHIKGSGWDMGSIEYPGLPAVELNPLKATLNLKKLN
                     DFDMVNLQRKCLLNSSSPNPSVETLLHAFLPHTYVDHTHASAILSLIDQPNNIKICQD
                     AFGDNVGIVPYVIPGFELAKIAYKVY"
BASE COUNT          939 a          457 c          482 g          919 t
ORIGIN      
        1 cattctccaa tgcagcagga ataagaatat cacaatcata agatagtaaa tcggcagagt
       61 tcttgatgga cttagtagtg ggaaaattta aaatcgtatt attttctaat tggtattttt
      121 ccaattcctt aatgtcaatg cccttgtcat taaatattcc tccatctttt tcggcaatac
      181 ctataatttt agcaccattt tgaaataatg cctttgcgga attaaatcct acatttccaa
      241 atccttggat aataatgttt tgatcactta attccttatt tactcccgct cgctgaactt
      301 ccgcagcatg acgaaaaaat tcttttaacg cttcacaaac ccctctcccc gttgcttcta
      361 aacgtccttt tatccctccg tgatctactg attttcctgt aacgcaagcc atgtgattga
      421 tgtcatcagg gcgtagactt ttatatgcat ctagtatcca ccccatttct ctttgtgatg
      481 tgcccacgtc tggtgctggt acgtctcttg cgggacttag atagcctctc cggattaatt
      541 ctcttgcaaa ttttttagtt attttttcca atgattcttc atcatacttt tgcggatcaa
      601 ttaataatcc tcctttggca ccgccaaaag gaacatctac cactgcacat ttaaacgtca
      661 tgagagaagc gagtgcttca acttcttctt gattcacatt agtggaaaat ctcatacccc
      721 ctttaactgg caacctatga ttagaatgca cagctcgcca accatgaaat attttaattt
      781 ctccttttaa tttaatggga aattttactt gcagaacaga gcggcaagtt tttaatattt
      841 ttgctatatt gggatcaata tcaatatgat taagggtatc gtttaccatc atattaacat
      901 tttccaaaaa agaaggtttt ttatcttgct ttagcatcta tgtttttaat ttatattgat
      961 tttaatcaac aatccacaaa gggatattct tatatgatat tctcgttgtc aaacactata
     1021 ataatagcta aatcagctgg tattttatgg taaatgttat gtcttttaaa tcaaggcttg
     1081 gttggatatc tgccgtggaa aaaaataatc aaatcatttc agtatcattt ggaaaatcga
     1141 agaccaaagg ctcttcaaca gaattaaaaa aattacaaaa aatattaagt aattattttt
     1201 ctgggaaaac aaaaaaaatt acttcaaata ttttaatgga gggcactaaa cttcaaatta
     1261 agatttggaa agagttgcaa aaaattcctt atggacaaac aaaaagttat ggagagattg
     1321 ctaaaatagt taaaacgtct cctcgatatg taggaaatgt atgtggacaa aataaccatt
     1381 tattactggt tccttgccat cgtgttgttc gcagtgatgg aaatctcggg ggtttttcag
     1441 gactaggagg tttaactttg aaaaaaaatt tactcgattt agaacaatca attgatgaat
     1501 aaattcttga ttatagtttt atgaaaataa ttatcctaca acacattgct tgcgagacac
     1561 ctggatacat taaagatttg atgattaaag atgggcttga tttaaccact attgaattgg
     1621 atgagggtga aacgatacct gatgacatta agcagtttga tggaatgttt tgcatgggcg
     1681 gtccaatgga tacctggatg gaaaaagact atccttggtt agttgaggag aaaaaaaaga
     1741 ttaaggaatt tgttgttgaa ttagagaaac cctttttagg tttttgctta ggatgtcaat
     1801 tgttaggaga ggtagtagga ggacaagttg tcaagtcatc gcctccagaa attggcattt
     1861 tggatattca gatgggagaa gaaaaaaagg aagatcatct attttctaga tttccaaatt
     1921 ccattaaagc gttacagtgg cattcctatg aagtagcggg attagaacgg aacaatgatg
     1981 ttacgttact tggctcttcg cccttaacca aatatcaaat ttttaaatac aaagatcatg
     2041 cttatggaat acaatttcac attgagatta aagataatac tgtcagcaat tgggggtgtg
     2101 tacctgaata caaaaatgct ttggaggaaa gtttaggaaa gggggcttta aataaatttg
     2161 agacgattgc agcacaaaac atgaaagata tgaataatct tgccactata gtatataaaa
     2221 attttttaga tatttaaaat gaaaaataag tggaattcaa tagaagaaaa aaaatatatt
     2281 aaaaaatata aaaacaatca tatccctcaa gacttagcgt tacggattta taccactcac
     2341 ttattaggga gagaaaaaac attggtgtta catggaggag gtaatacatc tcttaagaca
     2401 acttctaaaa atatttttaa taaaaaaata gatataatgc atattaaggg tagtggttgg
     2461 gatatgggct caattgaata tccagggtta ccagcagtag agctaaaccc tcttaaggca
     2521 acccttaatc tcaaaaaact taatgatttt gacatggtta accttcaacg aaaatgttta
     2581 ctcaactcct cttctcccaa tccttcggtt gaaactttat tgcatgcatt tttaccgcac
     2641 acttatgttg accatacaca tgcaagtgca attttgagtt taattgatca gcctaataat
     2701 attaaaattt gtcaagatgc atttggggat aatgtcggca tcgttcctta tgtaatacca
     2761 ggatttgaat tagcaaaaat agcttataaa gtttata
//
LOCUS       DTVV01000360            1505 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2045224, whole genome shotgun sequence.
ACCESSION   DTVV01000360 DTVV01000000
VERSION     DTVV01000360.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2045224"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            378..698
                     /gene="trxA"
                     /locus_tag="EYO86_04265"
     CDS             378..698
                     /gene="trxA"
                     /locus_tag="EYO86_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIC42279.1"
                     /translation="MSTNSTNDSTFDQDIKSSNIPVVVDFWAEWCGPCKQIGPILEEI
                     SEEKKSTVRILKLNIDENPEIPQKYNVRGIPTLMLFKEGKLIDTKVGSLPKSALSDWI
                     DSFL"
     gene            complement(693..1283)
                     /locus_tag="EYO86_04270"
     CDS             complement(693..1283)
                     /locus_tag="EYO86_04270"
                     /inference="COORDINATES: protein motif:HMM:PF02875.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42280.1"
                     /translation="MKTNSKKFTLRYKKIHYSINNPNLLGNHQIENASLAVAAILRLN
                     ELGYNFSNRIINQGIFKTKWAGRLEKGQLNNIPVYLDGAHNPGGAIQLLKFFQKQKNK
                     CWLIFGMLNNKDLLSYLKIIKPITLGVIAIKIKGEKNSFTPQQISMNCSKLNIACFEK
                     KSIKDANNFLTTKVMPKNILICGSLYLVGKIRYLYL"
BASE COUNT          521 a          196 c          209 g          579 t
ORIGIN      
        1 atgatgttaa aaatttaatt aataattttt catcccagga tttatgtaaa ttacttttag
       61 ttgaaaaaaa atatgcattt ggtttaaaaa aaaatactgg agactttact atgagcaatg
      121 gcaaagttag aagatatgtg gatggtgatc aaattattta ttattctggt atgcagatga
      181 ttaatttaga agttttcaat aatatagaag aaaataaatt ttcactaaat actgtttgga
      241 attctttaat tcaaaaaaat ttattaatgg gccaattgat gtcatctaat tggtttcatg
      301 ttggagatac acagggatta aatatattta ataaaataaa ctcttgaata tatttattta
      361 atgcttaaat ttattttatg tccacgaact caacaaatga tagtacattt gatcaagata
      421 tcaaatcttc aaatattcca gtagttgtag atttttgggc agaatggtgc ggaccttgca
      481 aacaaatcgg acctattctt gaggaaattt ctgaggaaaa aaaaagtaca gtaagaatat
      541 taaagctaaa tatagatgaa aatcccgaga tacctcaaaa atataatgta agaggaatac
      601 caactctaat gttgtttaaa gaaggaaaat taattgatac aaaagttggt agtttaccca
      661 agtctgctct tagcgattgg attgatagtt ttttataaat aaagataacg tattttccca
      721 actaaataaa gtgagccaca tatcaaaatg ttttttggca ttacttttgt ggtaagaaaa
      781 ttatttgcat ctttgattga ttttttctca aaacaagcaa tatttaattt actacaattc
      841 atactaattt gttgtggtgt aaagctattt ttttctcctt taattttaat tgctataacc
      901 cctaaggtta taggttttat tattttcaaa taagataaga gatctttatt atttaacatt
      961 ccaaatatta accagcactt gtttttttgt ttttgaaaaa attttaataa ttgaattgct
     1021 ccaccagggt tatgagcacc atctaaatat accggaatat tatttaattg acccttttct
     1081 aatctacctg cccattttgt tttaaaaata ccttgattaa ttattctgtt ggaaaaatta
     1141 taaccaagtt catttaatcg taaaatcgca gcaactgcca agctagcgtt ttcaatttga
     1201 tgatttccca aaagattcgg attattgata gagtaatgta tttttttata tcgcaatgta
     1261 aattttttag aatttgtttt cattatttta aattgtttgc cataaaataa tttagtattt
     1321 ttcttttttt taattttttc ttttatatat gaagttatta tttttttttg cttacctata
     1381 attatattac tattttaaaa caacatctct cagcttgctc cagtccaaat ctcgtgtgaa
     1441 tccgagcatt ttgaacatgc atgggcacat acacactgct ccttccatat aacctggggt
     1501 tggac
//
LOCUS       DTVV01000361            1513 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2045914, whole genome shotgun sequence.
ACCESSION   DTVV01000361 DTVV01000000
VERSION     DTVV01000361.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2045914"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="EYO86_04275"
     CDS             complement(<1..458)
                     /locus_tag="EYO86_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HIC42281.1"
                     /translation="MIQIKIDNNIVKVKPGMTILQACQQLGIEIPIFCYHEKLSIAGN
                     CRMCLVEVKDNPKPVASCSIPVIDGMEIKTSSTMVRKARNGVMEFLLINHPLDCPICD
                     QGGECDLQDIAMSYGRDKSRFNYNKRAVKEKNFGPLIKTVMTRCIHCTRC"
     gene            complement(458..>1513)
                     /gene="nuoF"
                     /locus_tag="EYO86_04280"
     CDS             complement(458..>1513)
                     /gene="nuoF"
                     /locus_tag="EYO86_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIC42282.1"
                     /translation="DAGGDYFADESEPGTCKDRDVIRYEAHKLLEGCLLSSFALGVKT
                     CYIYIRGEFYNESKILQAAIDEAYQNKLLGKNACNSSWNLDIFLHRGAGAYIAGEETA
                     LLESLEGKKAQPRLKPPYPASTGLYGCPTTVNNVETIAAIPDILKKGAIWFSGIGKPN
                     NTGSKIFCISGHINKPCNVEEELGIPLKELIEKYAGGVIGGWNNLLAVIPGGSSVPLI
                     NKKIADSVLMDFDSLEEVNSSLGTAGVIVMNKSTNLLKAIARISRFYKKESCGQCTPC
                     REGAGWMWRVMERFVNGEGTLEEIDTLYDVSKEVEGHTICALGDAAAWPIQGLIRHFR
                     PLIEKKIKENNFKNKVA"
BASE COUNT          459 a          306 c          199 g          549 t
ORIGIN      
        1 atacatcttg tacagtgaat gcatcttgtc atcactgttt taattaaagg tccaaaattt
       61 ttttctttaa ctgctctttt attataattg aatctacttt tatctcttcc atagctcatt
      121 gcaatatctt gtagatcaca ctcgccacct tgatcacaaa ttggacaatc taacggatga
      181 tttattaaca aaaattccat aacaccattt ctagcttttc taaccatagt tgaagatgtt
      241 tttatttcca taccatcgat aacagggata ctacatgaag ctacgggttt tggattgtct
      301 ttaacctcta ctaaacacat acggcaattg ccagcaatag ataatttttc atggtaacaa
      361 aatattggaa tttctatgcc aagttgttga caagcttgta aaatagtcat gccaggttta
      421 actttgacta tattattatc tatttttatt tgtatcatta agcaacttta tttttaaaat
      481 tattttcttt tatttttttt tcaattaatg gacgaaaatg tctgatcagt ccttgtattg
      541 gccaagctgc tgcatctccg agtgcacaaa ttgtatgccc ctcaacttct tttgaaacat
      601 catatagagt atctatctct tcaagagtac cttctccatt aacaaatctt tccattactc
      661 tccacatcca acctgcgcct tccctacaag gtgtgcattg gccacaactt tcctttttgt
      721 aaaatctaga tattcttgct attgctttta ataaatttgt agatttattc attactatta
      781 cacctgctgt acctaaggaa gaattaactt cttctaaact atcaaaatcc attaaaacac
      841 tatcagcaat ttttttattt attaatggaa cagatgaacc tcctggaata acagctaaca
      901 aattattcca tccgcctatt acaccaccag catatttttc tattaattct tttaaaggaa
      961 tacctaactc ttcttcaaca ttacatggtt tattaatatg tcctgaaata caaaaaattt
     1021 ttgatcctgt gttatttggt tttccaatac cagagaacca tatagctcct ttttttaaaa
     1081 tatctggaat agcagcaatt gtttcaacat tgttaacagt tgttggacaa ccatatagtc
     1141 cagtagaagc tggatatggt ggtttaaggc gaggttgtgc ttttttgcct tctaaacttt
     1201 caagaagagc tgtttcctca ccagcaatat aagctcctgc accgcgatgt aaaaaaatat
     1261 ctaaattcca tgaactatta catgcgtttt taccaagcaa tttattttga taagcctcat
     1321 ctattgccgc ttgcaagatt ttagactcgt tatagaactc acctcttata tagatataac
     1381 aagttttaac accaagggca aatgaactaa gaaggcatcc ttcaagtaat ttgtgcgctt
     1441 catatcgtat aacatctcta tctttacaag ttcctggttc actttcatct gcgaagtaat
     1501 ccccgccggc atc
//
LOCUS       DTVV01000362            1293 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2051072, whole genome shotgun sequence.
ACCESSION   DTVV01000362 DTVV01000000
VERSION     DTVV01000362.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2051072"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="EYO86_04285"
     CDS             complement(<1..926)
                     /locus_tag="EYO86_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIC42283.1"
                     /translation="MNSIYNNFVEAIGNTPLIRLNGPSKETGCNILGKAEFLNPGGSV
                     KDRAALAIIKEAEENNIIKKGGTIVEGTAGNTGIGLTLIANSKGYKSIIVMPETQTKE
                     KIDTLRSIGADLRLVPAKPYKDPDNYIKFSEKLAKEIYKKNNGNTIWANQFDNTANLN
                     GHYNNTGKEIWNQTKGKIDGFICSSGTGGTIAGVGKALKEKNNKVKIYLSDPKGSALY
                     NFIKFNELKIEGNSITEGIGNSRITANFKEAPIDGAYSINDHEALTEIYKLIENEGLC
                     LGTSSGINIAGAIKLAKELGPGKTIVTILCDKS"
     gene            complement(929..>1293)
                     /locus_tag="EYO86_04290"
     CDS             complement(929..>1293)
                     /locus_tag="EYO86_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HIC42284.1"
                     /translation="FIDHGMGVVIGETSEISDNVTIYHGVTLGGISPAENSKEQVNVK
                     RHPTIKDNCIIGSGAQILGPVVLEKNARIGANTVILKNVPENGTMIGNPARNINKNQK
                     IDENFKPYGVEVKSNTQD"
BASE COUNT          362 a          240 c          183 g          508 t
ORIGIN      
        1 gatttgtcac aaagtatagt gacgattgtt tttccaggcc ctaattcttt agctaatttt
       61 atcgctccgg caatattgat gccactactt gtccctaaac ataatccttc attttctatt
      121 aatttataaa tttctgttaa agcttcgtgg tcatttatac tgtaagcacc gtcaataggt
      181 gcttctttaa agttggcagt gattctagaa tttccaattc cttcggtaat tgaattaccc
      241 tcgatcttaa gttcgttaaa tttaataaaa ttataaagag cagaaccttt aggatcggat
      301 aaataaattt taactttatt atttttttct tttaaggctt ttcctacacc tgcaatagtg
      361 cctcccgtgc cagaagaaca aatgaaacca tctattttgc cttttgtttg attccaaatt
      421 tctttaccag tattattgta atgtccattt aagtttgctg tattatcaaa ctgatttgcc
      481 catatagtat taccgttatt ttttttgtaa atttccttag ctaatttttc agaaaacttt
      541 atatagttat ccggatcttt atatggttta gctggaacta atcttaaatc tgctccaatt
      601 gatctgagcg tatcaatttt ctctttcgtt tgggtttcag gcattacaat aatagattta
      661 tatccttttg aattagcaat tagagttaat cctattcctg tattcccagc cgttccttca
      721 acaattgttc ctcctttttt aataatatta ttttcttcag cttctttgat tattgctaaa
      781 gctgctctgt ctttaactga accacccggg ttaagaaatt cagcttttcc aagaatatta
      841 cagcctgttt ctttagaagg accgtttaat cttattaaag gtgtgttgcc tatagcttca
      901 acaaaattat tataaattga attcacaatt agtcttgagt gttgctttta acttcaacac
      961 cgtatggttt aaaattttca tctatttttt gatttttatt aatgtttcta gcagggttgc
     1021 caatcatagt tccattttca ggtacatttt ttagaatgac ggtatttgct cctatccgag
     1081 cattcttttc taagacaaca gggccaagaa tttgtgcgcc agaaccaata atacaattat
     1141 ccttaatagt tggatgccgc ttaacattta cttgttcctt cgagttttca gctggagata
     1201 ttccgccaag agtaactccg tgataaatag taacattgtc tgatatttct gaagtctcac
     1261 ctataactac acccattcca tgatcaataa aaa
//
LOCUS       DTVV01000363            3527 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2053166, whole genome shotgun sequence.
ACCESSION   DTVV01000363 DTVV01000000
VERSION     DTVV01000363.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2053166"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            609..1559
                     /locus_tag="EYO86_04295"
     CDS             609..1559
                     /locus_tag="EYO86_04295"
                     /inference="COORDINATES: protein motif:HMM:PF14311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42285.1"
                     /translation="MQKTGCPCCARRKACPDNNLEFLRPSLAEEWHFEKNSPLLPSEV
                     MPNHNKKVWWLCEYSHPYDATPNNRNYGKGCPYCSGQKVGYVNSLADSFPEIAKEFNK
                     KINGKLKPKGILKSSNEVIWWVCKKNKEHEWPAAVSSRTIQKTGCRYCSGQAVSEDNN
                     FAVINPEKIKYFDFKKNKGITPYDYTSGSGVEVWWKCENRHSWEAPFKRISGGSGCNK
                     CSVQTSFPEIRLFCEINSTFEKTKWRHKFEKFEVDVLLEDYKIALEYDGWFFHENRLN
                     KDLKKNAYLEEKGISIFRIRQSPLKQIINDDVIAKIKKRT"
     gene            1790..2200
                     /locus_tag="EYO86_04300"
     CDS             1790..2200
                     /locus_tag="EYO86_04300"
                     /inference="COORDINATES: protein motif:HMM:PF14311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42286.1"
                     /translation="MFEPNSGKTVWWICKKNHEWDATIDKRSNGRNCPYCSNKKVCDD
                     NNLLAISPKISKEWAEELNGEKTPENTLNGSGYKAWWICSKGHYFHKRVVERTGKRVK
                     SGERYGNCPWCRGYRKYKIYVAPDIEKIKRELKK"
     gene            complement(2208..2477)
                     /locus_tag="EYO86_04305"
     CDS             complement(2208..2477)
                     /locus_tag="EYO86_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIC42287.1"
                     /translation="MNPEQIKKDWESRGYSFGIFKDPPGQVWADFVHKTDELVVLAEG
                     EIEIEIEGKSQQPPIGREVFIPANAMHTVRNVCKTNNVWYYGYKR"
     gene            complement(2474..2752)
                     /locus_tag="EYO86_04310"
     CDS             complement(2474..2752)
                     /locus_tag="EYO86_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42288.1"
                     /translation="MISIRAKSIDYFAQDKVKVSSGKYISDPFSDLGKPLSKTTYSIG
                     ISSSYMNLQPKLIRNLLGDSGEYIPKDIRKEAPDGSYTVYYQVKRILK"
     gene            complement(2756..3376)
                     /locus_tag="EYO86_04315"
     CDS             complement(2756..3376)
                     /locus_tag="EYO86_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42289.1"
                     /translation="MNCETKSLKNKLLLMKYFLSLIVLIISFSNLLAQSWSDLELSDS
                     VEKYKSFLVNRDSDGSCYVKQSYDKDPQKMELAYNGYYPYINIPFWNGIEGDVFYWVD
                     EGSGYSIRVSELKKNNISPKLNQNIIPEMKKGRALYVRVNPVGFPPRIQKFSLIGFTA
                     ALKRISRKDCLENKGDSFTSNLEVNFDNLPSGKAVVSGKTSLPDGT"
BASE COUNT         1270 a          543 c          618 g         1096 t
ORIGIN      
        1 gtgtctatgc aaaccaaacc ggttctcttg atattttaaa tattattata atctaaaaat
       61 atctgaaatg tcaagttgtt ttgttgttat atgttccaat ttatgctacg tattggttta
      121 cataatctac caatagaaaa cttacttatc cagatattcc aaatatagaa cagactaaga
      181 ctaaacccct acctaattga tggtttaata tgaaaaacaa aaaaatacca ccgaattctt
      241 caagggaaat taggagagag gaatcattgg cgtttctcaa acctgaattt attcaatact
      301 gggatgaaga aaaaaatgaa aaagacattt ataaaattag accaacttat ggtgcttcta
      361 cgtggtagaa atgtaaaaag gggcattctt ttccgtcatc gccaaacact gttacaaata
      421 gagaagaatt aatttgtaat ttttgtaatt caataaagta tacctatcct gaactaattt
      481 tagagtggga ttggaaaaag aatgaaaaag attctgaaaa ttttttaact ggaagtaatg
      541 aaaaagtcca ctggaaatgt aaaaagaatg acattcatgg gtgggaaact actgttgctt
      601 cgagagcgtt gcaaaaaact ggatgtccat gttgtgctag gaggaaagca tgtcctgata
      661 ataatcttga atttttaaga ccaagtttgg ctgaagaatg gcattttgaa aaaaacagtc
      721 cattacttcc atctgaagta atgccaaatc ataataaaaa agtctggtgg ttatgtgagt
      781 attctcatcc ttatgatgca acccctaata atagaaatta tggaaaaggc tgtccttact
      841 gctccggtca aaaagtcggg tatgtaaatt ctcttgcgga tagtttccca gagattgcaa
      901 aagaatttaa taagaaaata aatggaaagc ttaaaccaaa gggtatcctt aaatcatcaa
      961 acgaagtgat atggtgggtc tgtaaaaaaa ataaagaaca tgagtggcca gctgcagtta
     1021 gttctagaac tatccaaaaa acaggttgta gatactgctc aggtcaagca gtgtcagagg
     1081 ataataattt tgcagtaatt aatccagaaa agattaaata ttttgacttt aaaaagaata
     1141 aagggataac tccatatgat tataccagtg gttctggcgt agaagtttgg tggaaatgcg
     1201 aaaatagaca ctcctgggaa gcacctttta aaagaatctc tggaggcagt ggctgtaata
     1261 aatgttctgt tcaaacatca tttcctgaaa taagattgtt ttgcgaaatt aattcaacat
     1321 ttgaaaaaac caaatggaga cataaatttg aaaagttcga agttgatgtt cttttagagg
     1381 attataaaat tgctctagaa tacgatggat ggttctttca cgaaaatcgt ttgaataaag
     1441 atcttaaaaa aaatgcatat ctggaagaaa agggaataag tatttttcga ataaggcaat
     1501 ctcctctcaa acaaataata aatgatgatg tgatagctaa aataaaaaaa aggacctaga
     1561 taaaaagtat ataaatcaaa ttttagtaaa aattattcaa tatgtttcaa aaaaagatca
     1621 ggaaaaaata aaggcatata ttgaacaaga ctcttattca gatgaaaaag agtttaaaag
     1681 aattgtatct tatcttccga agccaacccc agaaaatagt ttagctaaaa aaaatccaga
     1741 actttcaaga caatggaata agaaaaaaaa tgatcctctc actccggaaa tgttcgaacc
     1801 caatagtgga aagacagttt ggtggatttg taaaaaaaat catgaatggg atgctacaat
     1861 tgataaacgt tctaatggaa gaaattgccc atattgtagt aacaaaaaag tttgtgatga
     1921 taataatcta cttgctattt ccccaaaaat ttctaaagaa tgggctgaag aactgaacgg
     1981 tgagaagaca cctgaaaata cattaaatgg atcaggatac aaggcttggt ggatatgcag
     2041 taagggccac tattttcata aaagagtcgt tgaaagaact gggaaaaggg taaaatctgg
     2101 tgagcgttat ggtaattgtc cttggtgcag aggatatagg aaatataaaa tatatgttgc
     2161 accagatatt gaaaaaatta aaagagaatt gaaaaaataa ttttacttta tcttttgtat
     2221 ccataatacc agacattatt tgtcttacac acattcctaa ctgtgtgcat agcattagca
     2281 ggaataaata cttcccgacc aattggaggt tgctgtgact taccttcaat ttcaatctca
     2341 atttctccct ctgctaatac gacaagttca tctgtcttat ggacaaaatc tgcccaaact
     2401 tgtccgggag ggtctttgaa gattccaaaa ctgtaacctc tggattccca atcttttttt
     2461 atttgttcag gattcatttt aaaattcttt ttacttgata atatactgtg taacttccat
     2521 ccggtgcttc ttttctaata tctttaggga tatattcccc tgaatctccg agcaaatttc
     2581 ttattaactt cggttgcaga ttcatgtagc tagatgaaat ccctatgcta taggttgttt
     2641 ttgacaatgg ctttcccaaa tcactaaaag gatctgaaat atatttccct gagctgacct
     2701 ttaccttatc ttgggcaaaa taatcaatac ttttagcacg tattgatatc attagctacg
     2761 ttccatcagg taatgaagtt ttaccagaaa ctactgcttt tccgctgggc aaattatcaa
     2821 aattaacttc caggttagat gtaaaagaat ctcctttatt ttcaagacaa tcttttcttg
     2881 aaattctttt taatgccgct gtgaagccaa tcaaactaaa cttttgtatt ctaggaggga
     2941 atccaacagg attaactcga acataaaggg ctcttccttt tttcatttca ggtataatat
     3001 tttgattcaa tttgggagaa atattatttt ttttcaattc agaaacacga atagaatagc
     3061 cggaaccttc atcaacccag taaaagacgt caccctcaat tccattccaa aaaggtatgt
     3121 ttatataagg ataataacca ttgtaggcaa gttccatctt ttgaggatct ttgtcatagg
     3181 attgcttaac atagcaagaa ccatcagaat ctctattaac gaggaatgat ttatattttt
     3241 ctacagaatc acttagctcc aaatctgacc aactttgagc tagtaagttt gaaaaggaga
     3301 tgattaagac tattaatgat agaaaatatt tcattagtaa aagtttattt ttaagcgatt
     3361 ttgtctcgca gttcataatc aaaatatttg cactcttttg ggtatggcag gggggtgggg
     3421 tggcctatca gaaagtggca tataaaaacc ttttttatat gtacgataat gcagtttaac
     3481 gtacataatc ctttatcata caggtgtaat atcctttgca atttcca
//
LOCUS       DTVV01000364            2333 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2054107, whole genome shotgun sequence.
ACCESSION   DTVV01000364 DTVV01000000
VERSION     DTVV01000364.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2054107"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(68..1012)
                     /locus_tag="EYO86_04320"
     CDS             complement(68..1012)
                     /locus_tag="EYO86_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIC42290.1"
                     /translation="MTQEKQIHTVTGAFGYSGKYIAQRLLAKGYTVHTLTNSTDRHNP
                     FADKMTVHPFNFEHPEKLAESLQGVSVLYNTYWVRFNHKLFTHADAVKNTLILFEAAK
                     KAGVERIVHVSITNPATEPDLEYFQGKAHLEKSLVESGLSYAILRPAVLFGKEDILIN
                     NIAWALRRFPIFGVFGDGNYGIQPIYVDDLAKLAVEQGEIRENKIINAIGPESFTYRE
                     LVEEIGGIIEKNRPVIATPPVVGYLVALITGWFVGDVFLTWDEIKGLMAGTLAVDTPP
                     AGSTKLTAWAKKHADTLGREYTSELARRKDRVQAYRSN"
     gene            complement(930..2303)
                     /locus_tag="EYO86_04325"
     CDS             complement(930..2303)
                     /locus_tag="EYO86_04325"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037959.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIC42291.1"
                     /translation="MDEIREKFIHRYENGIPQILRENLIADIETPISSLLKISEKQKY
                     SFLLESVEGGSKRGRYSLLGCEPDLIWEVKENKAHLHYTNNNYNYKLSSSPLESLKEL
                     IEISKLPRGNYEVPYPILVGYLGYPMIQYMENIKLNNSDNINIPEATLIRPKVVAVFD
                     NIKDTISLMTLTYPSKKISAIKAYNEAKNILDQTVKKLQKNSVDFYFNEISFENKLDF
                     KSNYTKQEYFNIIKKAKKYIEQGDILQVVTSQRFETKYDLKAIHLYRSLRRLNPSPYL
                     VNLNFDKIGLVASSPELMVQLRDQKVTIRPIAGTRKRGINSHEDMELSKDLMNDEKEL
                     AEHLMLLDLGRNDVGRVSSPNSVKVTEKMLVEYYSHVMHIVSNVEGKIDSKYEAVDAL
                     VAGFPAGTVSGAPKIRAMEIIEELENLNRSFYADCMGYFDSGKTDSYRNRSFWILRQV
                     HCPAAFG"
BASE COUNT          667 a          460 c          387 g          819 t
ORIGIN      
        1 cctaaatgtc tataaacaaa aaaccccgga tggggcttga ttgggcatgg tcgaaggggt
       61 ttgggcctta gttcgaccta tacgcttgta ctctatcttt tcggcgagct aactcacttg
      121 tatattctct gcctaaagta tctgcatgct ttttggccca agctgtcaat ttagtactgc
      181 cagccggggg tgtatccacg gccagcgtac cggccattaa gcctttgatt tcatcccagg
      241 tcagaaaaac atcaccaacg aaccagcctg tgatgagtgc cacaagataa ccaacgactg
      301 gaggggtggc tatgaccggt cgattttttt caatgattcc cccaatttct tcgacgagtt
      361 ctctataagt gaatgattca ggcccgatag cattgatgat cttattttct ctgatttctc
      421 cctgctcaac ggccagtttt gccaggtcat caacataaat tggttgaata ccataattac
      481 catccccaaa taccccaaag ataggaaatc gtcttaaggc ccaggcaatg ttattgatca
      541 gaatatcctc ttttccaaag agtacggccg gccgtaaaat tgcatatgac aggcccgatt
      601 ccaccaagct tttttcaaga tgggctttcc cttgaaaata ctcgaggtcg ggctcggtag
      661 caggattggt gatgctgaca tgaacgattc gctcgacccc tgcttttttt gccgcctcaa
      721 ataaaatcag ggtgttcttg accgcatcgg catgcgtaaa taatttgtga ttaaacctga
      781 cccagtaagt attgtacaac accgataccc cttgcaagga ttcggccaat ttttcggggt
      841 gttcgaaatt aaatggatgt actgtcattt tatcggcaaa gggattgtgc cggtcagtgg
      901 aatttgtcag ggtatgcacg gtatacccct tagccaaaag ccgctgggca atgtacttgc
      961 cggagtatcc aaaagctcct gttacggtat gaatctgttt ttcctgagtc aaaatatccc
     1021 atacaatcag cgtaaaagct tctatttaaa ttttcaagtt cctctattat ctccattgct
     1081 ctaattttgg gggctcctga aactgtacca gctggaaatc cagctactag ggcatctaca
     1141 gcttcatatt tactgtcaat ttttccttct acatttgata caatatgcat tacatgagaa
     1201 taatactcta ctaacatttt ttctgtaact ttaactgaat taggagaaga aactctcccc
     1261 acatcatttc ttcctagatc taaaagcata agatgttcag ctaattcttt ttcatcattc
     1321 attaaatcct ttgataattc catgtcctca tgagaattaa tgcctctttt tcgagttcct
     1381 gctataggtc ttattgttac tttttgatct cttagttgaa ccattaattc tggacttgaa
     1441 gctacaagac caattttatc aaaatttaaa tttaccagat atggagaagg atttaatctt
     1501 cttaatgatc tgtacaaatg tatagccttt aaatcatatt ttgtttcaaa tctttgagag
     1561 gttacaactt gcaatatatc tccttgctca atatactttt ttgctttttt aattatatta
     1621 aaatattctt gttttgtata atttgattta aagtccagtt tattttcaaa acttatttca
     1681 ttaaaataaa aatccactga atttttttga agttttttaa ctgtttgatc taaaatattt
     1741 tttgcttcat tataagcctt aatcgcactt atttttttgc tgggataagt taatgtcatt
     1801 aatgaaatag tgtctttaat attatcaaag acagcaacta cctttggcct gatcaaggta
     1861 gcttcaggaa tattgatatt atctgaattg ttcagtttga tattttccat atattgaatc
     1921 ataggataac caagataacc taccaatatt ggatatggta cttcataatt tcctcttgga
     1981 agttttgaaa tttcaattaa ttcttttaaa gattcaagag gagagctact cagtttataa
     2041 ttataattat tatttgtata atgtagatgc gctttatttt ctttgacctc ccatatcaaa
     2101 tcaggttcac acccaagaag agaatatctt cctcttttac tccctccctc aactgattct
     2161 aataaaaatg agtatttttg tttttcagaa atttttaata gagatgaaat aggtgtttca
     2221 atatcagcta tcaaattttc tcttaatatc tgaggtattc cattttcata tctatggata
     2281 aatttttctc ttatttcatc cattaatact gacttaaaag agcatctagt aag
//
LOCUS       DTVV01000365            1599 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2055008, whole genome shotgun sequence.
ACCESSION   DTVV01000365 DTVV01000000
VERSION     DTVV01000365.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2055008"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1599)
                     /locus_tag="EYO86_04330"
     CDS             complement(<1..>1599)
                     /locus_tag="EYO86_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIC42292.1"
                     /translation="PEEFFKEQIERTGHKTNDFPFAVGMVFLPRTDFADQEKSRIIVE
                     SEIIKGGFNILGWRHVPINTRIIGEKAKLTRPEIEQVLIGNDIFSNKVEIDNKLYLIR
                     KRIEKKIKQENINEFYVCSLSSQSIIYKGMFLAEQLSNFYPDIQDSRFISKFAVYHQR
                     YSTNTFPTWSLAQPFRVIAHNGEINTLKGNKNWMAAHEPRMRHANFENNIEDLKPIID
                     SNASDSAALDATIELLVRSNRSLPMAKLITIPEAFAHRRDFSKKLKDLYAYANAVMES
                     WDGPAAICGVHEDWAIAGMDRNGLRPIRYTLTKDFLVAGSETGMVDIEESEIIERGRV
                     GPGQMIAVNFQEGKFYHDSAIKSKLSQSRPFGEWTKKITHIDKLVQSVDEEFREIDSG
                     DLRKRMASFGWTVEDIELILHPMIADKKEAVGSMGDDTPLAVLSDNYRGLHHFFRQNF
                     SQVTNPPIDSLREKVVMTLRTRIGNLSNILDEDQSQCDHLQLNSPFLSINQFKTMRQY
                     MKDTVKIIDTTMNLNQKNYSFKEE"
BASE COUNT          482 a          302 c          243 g          572 t
ORIGIN      
        1 aattcctcct taaaactgta atttttttga tttaaattca ttgttgtatc aatgattttt
       61 acagtatctt tcatatactg gcgcattgtc ttaaactgat taatggaaag aaatggtgaa
      121 tttaattgta aatgatcaca ttgagactga tcttcatcaa gaatgttgct taagttgcct
      181 attcttgttc taagagtcat tactactttt tctcttaagc tatctattgg tggatttgta
      241 acctgactaa aattttgcct aaaaaaatgg tggaggcctc tataattgtc agataaaact
      301 gctaaaggcg tatcgtcacc catcgaacct acggcctctt ttttgtctgc gatcatcgga
      361 tgaagaatta attcaatatc ttcaacggtc cagccaaaag atgccatcct ttttctaaga
      421 tcgcccgaat ctatttctct aaattcttca tcaacagact gaactaattt atcaatatga
      481 gtaatttttt ttgtccactc accaaatggt cgagattggg atagcttgct ttttatcgct
      541 gaatcatggt agaattttcc ttcttggaaa tttaccgcta tcatctgtcc tggaccaact
      601 cgtcctcttt ctataatttc agattcttca atatccacca tacctgtttc tgatccagca
      661 actaaaaaat cttttgtgag agtatatcgt attggtcgaa gaccatttct atccattcca
      721 gcaatagccc aatcttcatg aactccacaa atagcagctg gaccatccca agattccata
      781 acagcgttag cataggcata taagtctttt aatttttttg agaaatccct tctgtgagca
      841 aaagcctccg gaatggttat taattttgcc atcggtaaag atctattaga acgtaccaaa
      901 agttctatag ttgcatcaag tgcggcagag tcagatgcat ttgaatctat tatgggcttt
      961 agatcttcaa tattgttttc aaaatttgca tgacgcatcc gtggctcatg tgcagccatc
     1021 caatttttat tacccttaag agtattaatt tctccattat gagcaataac cctaaaaggt
     1081 tgggctaatg accatgtagg aaatgtatta gttgaatatc gttgatgata gacggcaaac
     1141 tttgagataa acctactatc ttgaatatca ggataaaaat ttgataattg ttctgccaaa
     1201 aacatacctt tataaatgat tgattgtgat gataatgagc aaacataaaa ctcattgata
     1261 ttctcttgtt ttattttttt ttcaatacgt tttcttatta aataaagttt gttatcaatc
     1321 tcaactttat tgctaaaaat atcattgcca attaatacct gttcaatttc agggcgagtt
     1381 aactttgctt tttctccaat tattcttgta ttaattggaa catgcctcca accaaggata
     1441 ttaaaaccac cttttattat ttcggattca acaataattc gcgatttctc ttggtcggca
     1501 aaatctgtac gtggtaaaaa aaccattcct actgcgaaag gaaaatcatt tgttttatgt
     1561 cccgttctct caatctgctc tttaaaaaat tcttcagga
//
LOCUS       DTVV01000366            1338 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2064272, whole genome shotgun sequence.
ACCESSION   DTVV01000366 DTVV01000000
VERSION     DTVV01000366.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2064272"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            41..343
                     /locus_tag="EYO86_04335"
     CDS             41..343
                     /locus_tag="EYO86_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42293.1"
                     /translation="MYKTRQVNIRLGRDATRVGRYLLISDSYNLSPQEAVRLAKEYPR
                     LELFRIPEQNIPSEIIKDSYMLFDPLGNGILIYSPELPGGELLEDLKKVLKNSKIG"
     gene            519..1157
                     /locus_tag="EYO86_04340"
     CDS             519..1157
                     /locus_tag="EYO86_04340"
                     /inference="COORDINATES: protein motif:HMM:PF02630.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIC42294.1"
                     /translation="MKRNFTWTIVSCLAVMLVALSLYVNKMTTQVYLSNEQLKDLGLY
                     LIEPARNLGSFNLIDTNGKEFLPKDFKGKWNVLFFGFTFCPDICPITMSMLSRIEKEI
                     DSQELDKIRIFLVTVDPVRDNPSQLKIYLKNFSENFTGLTGGLDQIYNFATRVNAPFT
                     PINNSKDPYYTVDHTGSIVLIDPNGNYAGFFRAPHTQDKIKKALVEIIRRDL"
     gene            1154..>1338
                     /locus_tag="EYO86_04345"
     CDS             1154..>1338
                     /locus_tag="EYO86_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 family protein"
                     /protein_id="HIC42295.1"
                     /translation="MNSVEKQKNVFGEEIETCCESPITGFFRDGFCHTDDTDEGVHTI
                     CVSMTKDFLEFSKSKGN"
BASE COUNT          450 a          225 c          246 g          417 t
ORIGIN      
        1 cttcttaaac agcccctgtc aggagacttg ctggagttct ttgtataaaa caagacaagt
       61 aaatattagg cttggtagag atgcaacaag ggttggaagg tatctattaa tatcagattc
      121 atacaatctc tccccgcaag aggcagtaag actagcaaaa gaataccccc gtctagaatt
      181 atttaggata ccagaacaaa acattccttc tgaaataatt aaggattcct acatgttatt
      241 tgaccctttg ggaaatggga tattgattta ttcaccagaa ttgccagggg gtgagctttt
      301 agaggatcta aagaaggttt tgaaaaattc aaaaatagga taataaaaat aatgtcattc
      361 aggaaaataa cttctttctt aggacttgta tttctttatt aagctatagt gctctactag
      421 gatgatgaaa acaaaaaggg tatgcccacc ttcttttatt ctatctggta tttggctttt
      481 ctttttagtt caatgctaat agataacttt atatctctat gaaaagaaat ttcacttgga
      541 caattgtaag ttgtttagct gtcatgcttg ttgctttatc tttatacgta aacaaaatga
      601 caacccaagt ctatttaagc aatgaacaat taaaagatct gggcctttac ttaattgagc
      661 ctgcacgaaa cctaggttca ttcaatctta tagatacaaa tgggaaggaa tttcttccca
      721 aagattttaa gggaaagtgg aatgtccttt tttttggttt tacattttgt cctgatattt
      781 gtcctatcac catgagtatg ctttcaagaa ttgaaaagga aattgactct caagaactag
      841 ataaaatcag aatttttttg gtcacagttg atcccgtaag ggacaatcca agtcaactta
      901 aaatatatct aaaaaatttc agcgagaact ttacaggcct aaccgggggc ttggatcaaa
      961 tatataattt tgcaactaga gtaaatgctc ccttcactcc cattaacaat agcaaggatc
     1021 cttattacac ggttgatcat actggcagta ttgttttgat tgatcccaat ggtaattacg
     1081 ccggtttctt tagggcaccc cacacccaag ataaaataaa aaaggccctt gtagaaataa
     1141 tcagaagaga tttatgaatt cagtagaaaa acaaaagaat gtctttgggg aggaaataga
     1201 gacatgctgt gaaagcccca ttaccggctt tttcagggat ggattttgtc atacagatga
     1261 cacagacgaa ggtgttcaca ctatttgtgt aagcatgacc aaagactttt tagaattttc
     1321 caagtctaaa ggaaatga
//
LOCUS       DTVV01000367            1912 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2066229, whole genome shotgun sequence.
ACCESSION   DTVV01000367 DTVV01000000
VERSION     DTVV01000367.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2066229"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /gene="nuoN"
                     /locus_tag="EYO86_04350"
     CDS             complement(<1..1282)
                     /gene="nuoN"
                     /locus_tag="EYO86_04350"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HIC42296.1"
                     /translation="MQNNFLAIFMMPEITLVLLTTIILLIGLFQKKNSFSNVTNLSVI
                     ALFFVFFLIYLNREFSFASYNYFFKSSLLIQFFKSIVVLGSIASIIISKSYFKELNLS
                     RFEIPVLISFSTLGMLILISSNDLISMYLGIELQSLTLYVVAAIKRDSIQSAESGVKY
                     FVLGALSSGILLYGCSLIYGFTGTTNFEEIRLSLISINHLSLGIIFGLVFVLVGLAFK
                     VSAVPFHMWTPDVYEGAPTSITAFFAIVPKVSAVAIIYRFCLEPFGNFYSEWSQIIIF
                     LSISSMFLGAIAAIAQQSIKRLLAYSSIGHVGYILIGLASANAAGIKGVIIYMLIYVV
                     MNIAIFSIILSLKNNNSYIEKISELSGLSKSKPIISLSIAIIMLSLAGIPPFAGFFGK
                     FYIFIAAVEADLIFLAVLGVISSVISAYYYLRIIK"
     gene            complement(1272..>1912)
                     /locus_tag="EYO86_04355"
     CDS             complement(1272..>1912)
                     /locus_tag="EYO86_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIC42297.1"
                     /translation="SIIAIIYTSLVALAQTDIKKLIAYSSVAHMGMVTIGIFLVNQQG
                     IEGAIILMISHGVVSAALFLSVGVIYDRMHTLDISFYGGLVNNMPLYSVSFMIFVLAS
                     VGLPGTSGFVGEFLIIMGAFKYNSLIALFSATGIILSAVYMLYLYKRIIFGQMSNVKL
                     KDILDIDFREKIILYPLIIMIILIGIFPNIFLDPMRLPIELIINNYEFANAK"
BASE COUNT          775 a          307 c          264 g          566 t
ORIGIN      
        1 ctttaataat tctaaggtaa taataagctg aaatgacact agatattact ccaagaactg
       61 ctaaaaaaat taaatcagct tctacagctg caataaatat ataaaattta ccaaaaaaac
      121 ccgcaaaagg aggtattcca gctagagaca acattattat tgctatggaa agacttataa
      181 ttggttttga tttgctcaat ccggataatt cacttatttt ttcaatgtaa gagttattgt
      241 ttttaagtga taaaatgatt gaaaaaattg cgatattcat aactacataa attaacatat
      301 aaataataac gcccttaatt cctgcagcat ttgctgaagc gagacctatt aatatgtagc
      361 caacgtgacc aattgagctg tacgccaata atcgtttaat actttgttgg gcaattgctg
      421 ctatagcacc taaaaacatg gagctaatag acaaaaaaat aattatttga ctccactcgc
      481 tatagaaatt gccaaatggc tctaagcaaa atctatatat tattgctaca gcagatactt
      541 ttggaactat tgcaaaaaaa gctgtgattg aagttggagc accttcataa acatcaggtg
      601 tccacatatg aaaaggcaca gctgacactt tgaaagctaa tccaaccaat acaaaaacaa
      661 ggccaaatat tatacctaag cttaaatgat tgattgaaat taaagataag cgaatttctt
      721 caaaattagt tgttccggta aatccataaa ttaaagaaca tccatacaac agaataccag
      781 aagataaagc acccaaaaca aagtatttaa cacctgattc agcagattgg atagaatccc
      841 ttttaattgc agcaactaca taaagtgtta aactttgtaa ttcaattcct agatacattg
      901 aaattaaatc attcgaagaa attaatatta gcattcccaa agtagaaaat gaaattaata
      961 ctggtatttc aaaccgagaa agatttagtt ccttaaaata agatttagaa ataataatac
     1021 ttgctatact tcctaagaca acaatagatt taaaaaattg aattaataaa gagctcttaa
     1081 aaaaataatt gtaactagca aaagaaaact ctctattcaa ataaattaag aaaaatacaa
     1141 aaaataatgc aatgacggaa agattggtaa cattagaaaa agaatttttt ttttgaaata
     1201 agccaattaa taaaatgata gtcgtaagca gaactaaagt aatttcaggc atcataaata
     1261 tagctagaaa attattttgc attcgcaaac tcataattgt taataatcaa ttctattggc
     1321 aaacgcatag gatctaaaaa aatattaggg aaaataccta ttagtatgat cattattatc
     1381 aatgggtata aaataatttt ttcacgaaaa tctatatcta atatatcttt caattttacg
     1441 ttgctcatct gtccaaaaat aatcctttta tataaatata acatataaac tgctgacaat
     1501 atgatacctg ttgcggagaa gagggctatt aaagaattgt atttaaaagc tcccataata
     1561 attaaaaatt ctccaacaaa gccactcgtt ccaggcagac ccactgaagc taatacaaaa
     1621 atcataaaag atactgagta aaggggcata ttattaacta gccctccata aaagctaata
     1681 tctagagtat gcatacgatc ataaattact cctacactca aaaaaagggc tgctgaaact
     1741 actccatgac tgatcattag tattatagct ccttcaattc cttgctggtt aacaagaaaa
     1801 ataccgatcg tcaccattcc catatgtgct acagatgagt aagcaattag ttttttgata
     1861 tcagtttgag ctagagcaac tagggatgtg taaattattg caataatact ca
//
LOCUS       DTVV01000368            1922 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2070759, whole genome shotgun sequence.
ACCESSION   DTVV01000368 DTVV01000000
VERSION     DTVV01000368.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2070759"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            143..1225
                     /gene="galK"
                     /locus_tag="EYO86_04360"
     CDS             143..1225
                     /gene="galK"
                     /locus_tag="EYO86_04360"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIC42298.1"
                     /translation="MENNIKNFKNIFAEDIVSQAFAHARVNLIGEHTDYTGGYVLPSL
                     LPYKTSVYISKNRNATYAVYSEFFKKKIEFNDFMKSKTNEWVDYVKGCLFVFFDENVK
                     IKNAYINIYLESDIPMGRGIASSSALCVATLKALNKFFGTSFSDKHIAVLAQKVERNY
                     IGVSGGIMDQMVSSIGIHGKAFFLDCLSLKYEFLEIPLNWQFCLIDSAVQRNLRDSSY
                     NERHEQLKNAEKILGVEYLGSVKLSTFNNNDFSDEVIYKRAKHVVTENDRVLNAKKYI
                     QNNNLSQFGELMNLSHYSYSNDFEASTEDVDLITKRSVESGAAGSRLTGGGFGGFTVS
                     LIEKTNYGLWYKNMRKHYSEDKFFTV"
     gene            1225..>1922
                     /locus_tag="EYO86_04365"
     CDS             1225..>1922
                     /locus_tag="EYO86_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC42299.1"
                     /translation="MEIYRNYINGEFRDSYSKKSFRSIDPSTEQPWSEIAEAQEKDVN
                     DAVNAAHEAFHGKWSKFLPQQRGNFLRAIGDQLKENAELLGTIETRDTGKMYKETKFQ
                     ANYIAEFYYYYAGLVDKIEGSTLPIDKQDMQVFTTRVPVGVVAAIIPWNSQMFLTAVK
                     LAPALAMGNTIIIKASEIAPAPLFEFAKLIDKINLPKGVINIITGFADECGKILTSHP
                     KVNRIAFTGGMHTA"
BASE COUNT          669 a          286 c          356 g          611 t
ORIGIN      
        1 caagcttaaa cataatttta attgggaaaa attattgtta gattgattat ttatttttat
       61 ttcaagtaag ttggcgctat tgaatttagg ggtaacaaaa ataaaagtca tagtttatta
      121 aatactgagc ataacaaata aaatggaaaa taatatcaag aattttaaaa atatttttgc
      181 tgaagatatt gtctctcaag cttttgctca tgcacgtgtt aatttgatcg gagaacatac
      241 ggactataca ggcggttatg tactaccgtc tctattgccg tacaaaactt ccgtttatat
      301 ttctaaaaat agaaatgcaa cctatgctgt gtattcggaa ttttttaaaa aaaaaattga
      361 atttaatgat tttatgaaat caaaaacaaa tgaatgggta gattatgtaa aaggatgttt
      421 gtttgttttt tttgatgaaa acgtaaaaat aaaaaatgca tatataaata tatacttgga
      481 atcggacatt cctatgggaa gaggaattgc atcatcctcc gctttatgcg ttgctacgct
      541 gaaagctctc aacaaatttt ttggcacttc tttctctgat aagcacattg ccgtactcgc
      601 acaaaaagtg gaaagaaatt atattggagt ttcaggaggc ataatggatc aaatggtatc
      661 ctcaattgga attcacggta aggcattttt tcttgattgt ttgtcattaa aatatgaatt
      721 tctagaaata cctttgaatt ggcaattctg tttaattgat tctgcagttc aaagaaactt
      781 acgggatagc tcgtataatg agagacatga gcagttaaaa aatgcagaaa aaatacttgg
      841 ggtagaatat ctcggcagtg taaaactaag tacttttaat aataatgatt ttagtgatga
      901 ggttatttat aaaagggcga aacatgttgt tactgaaaat gatcgtgttt taaatgctaa
      961 aaaatacatt caaaataaca atttatccca atttggtgaa ttaatgaatt tgagccatta
     1021 ttcctactca aatgactttg aagcatcaac agaagatgtg gatttaatca ctaagcgttc
     1081 tgttgaaagt ggcgccgcag gatcaaggtt aactggcgga ggatttgggg ggtttacagt
     1141 atcattgatt gaaaaaacca attatggttt atggtataaa aatatgcgaa aacattattc
     1201 agaagataaa ttttttacag tgtaatggaa atttatcgaa actatattaa tggtgaattc
     1261 agagattctt actctaaaaa atccttccgt tcgattgacc cttcaactga acagccttgg
     1321 tccgaaattg ctgaagctca ggaaaaagac gtcaatgacg ctgtgaatgc tgctcacgaa
     1381 gcttttcatg ggaagtggtc aaaattttta ccccaacaac gcggcaattt cttaagagca
     1441 ataggagatc agttaaaaga aaatgcggaa cttcttggca caatagaaac tcgtgatacc
     1501 ggtaaaatgt ataaggagac taaatttcaa gcaaattata ttgctgaatt ttattattac
     1561 tatgcaggat tggttgataa aattgaaggt tcaactcttc ccatagataa acaggatatg
     1621 caagtattta caacccgcgt tcctgtggga gttgttgctg ccattattcc ttggaactca
     1681 caaatgtttc tcactgctgt aaagttggct ccagccttgg caatgggtaa taccattata
     1741 attaaagcat cagaaatagc tcctgcacct ctttttgaat ttgctaaact aattgataaa
     1801 ataaatttgc ccaaaggagt aataaatatc atcaccggtt ttgctgatga gtgcggaaaa
     1861 attttgacat cacatcccaa agttaaccgc atagctttta caggcggaat gcacacagcc
     1921 aa
//
LOCUS       DTVV01000369            1155 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2073911, whole genome shotgun sequence.
ACCESSION   DTVV01000369 DTVV01000000
VERSION     DTVV01000369.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2073911"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(135..677)
                     /gene="def"
                     /locus_tag="EYO86_04370"
     CDS             complement(135..677)
                     /gene="def"
                     /locus_tag="EYO86_04370"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:PRK00150.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIC42300.1"
                     /translation="MSILKIARLGHPILLQKAKIVEDITSNKTKKIIQDMTETMLDAK
                     GIGLSAPQIHINRQIIIFRVPEEDDEKKIKITALINPGFTKITDKTENEWEGCLSIPG
                     MLGLVKRFSKIQYEGSDMRGNIIQRKAEGLHARIVQHEYDHLQGILYTSRLVDNSAFG
                     YAEEIEEYWKKKEIEKSQSQ"
     gene            779..979
                     /gene="rpsU"
                     /locus_tag="EYO86_04375"
     CDS             779..979
                     /gene="rpsU"
                     /locus_tag="EYO86_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIC42301.1"
                     /translation="MSLTVNVKDNNIEQALRNLKKKLQREGLFKEMKLRKHYEKPCLK
                     KAREKEENIRRSRKSARLRHSD"
     gene            complement(994..>1155)
                     /locus_tag="EYO86_04380"
     CDS             complement(994..>1155)
                     /locus_tag="EYO86_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_427268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD synthetase"
                     /protein_id="HIC42302.1"
                     /translation="ILDVEKSGTVLFIKRSLATGYSGVDNELFYRDNTTMLFGDAKKM
                     CENIVKALS"
BASE COUNT          382 a          198 c          156 g          419 t
ORIGIN      
        1 acccttcagg aaataaggcg gctatttctg aaaatttaaa ttttgattta gccgctatat
       61 tgtaaaaaag tttatcattc catccacact tagaaacatg ttcttttgct tttaatagga
      121 tctgatatct taatttattg agattgactt ttttcaattt ctttcttctt ccaatattct
      181 tcaatttcct cagcatatcc aaaagctgaa ttatcaacca aacgactcgt atatagaatg
      241 ccctgcaaat gatcatattc atgttgaaca atacgagcat gcaatccttc tgctttcctt
      301 tggataatat tgcctctcat atcagatcct tcgtattgaa ttttagaaaa tctctttact
      361 agacccaaca ttcctggaat agataagcaa ccttcccatt cattctctgt tttatcagtg
      421 atttttgtaa atcctggatt aatcaaagct gtgattttta tctttttttc atcatcttct
      481 tccggaactc taaatattat tatttgtctg tttatatgta tctgaggtgc tgaaaggcca
      541 atccctttgg catcaagcat cgtttccgtc atatcttgaa ttattttttt tgttttattg
      601 cttgttatat cttcaactat tttagctttt tgaagcaaaa tagggtgtcc taaacgggca
      661 attttcaaaa ttgacattag tgcttataca aagccataaa ttaattttaa atacataaat
      721 ttggatattt gcataaattt atacattatg ctaattgctt cactctttta aggaaaaaat
      781 gagtttaaca gtaaatgtaa aagataataa tattgagcaa gctcttcgta atttgaaaaa
      841 aaaattgcaa agagagggtt tatttaaaga aatgaagctt cgtaaacact atgaaaagcc
      901 ttgtttaaaa aaagctcgtg aaaaagaaga aaatattaga agatctcgta aatcagcaag
      961 actaagacat agcgattaaa ttaaccgagc aagtcaactc aaggccttca ctatattttc
     1021 acacatcttt ttagcatcac caaaaagcat agttgtatta tctctgtaaa aaagctcgtt
     1081 atcaacacct gagtatcctg tggctaaaga tctttttata aataatacag ttccggattt
     1141 ttcaacatct aatat
//
LOCUS       DTVV01000370            2151 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2081961, whole genome shotgun sequence.
ACCESSION   DTVV01000370 DTVV01000000
VERSION     DTVV01000370.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2081961"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="EYO86_04385"
     CDS             <1..112
                     /locus_tag="EYO86_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42303.1"
                     /translation="ENKNRLGLSFGHISNANLGDKNPGVEIISLSYQVPF"
     gene            complement(109..1101)
                     /gene="dusA"
                     /locus_tag="EYO86_04390"
     CDS             complement(109..1101)
                     /gene="dusA"
                     /locus_tag="EYO86_04390"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:PRK11815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIC42304.1"
                     /translation="MSKDKYSRKFCVAPMMGYTTPHARKLYRILSQKTFLFTEMIAAS
                     TMLHSKTKDLIIENDLQNPIALQVGGSDIDDLALCSKIAKSYNYDEINLNVGCPSKAV
                     QKGSFGACLMQDKKLVRACLETMQNASDIEVTMKCRIGLGNDLNYDYFSTFIDEIVQS
                     GINIIYVHARNAILNGISPKGNRSIPRLNYEFVKKIKKEHPHVQFILNGGIDSLDKAY
                     ALSKEHDGVMLGRLIQNNPFCLGKVDKLFFSFKGEKNLNKKIILDYFNYIKPKIGNDS
                     IFRLLSPLLQIFFGIPDSKKLKTEIHQQIKDQEIDNLESLFLQFINLQKKAILS"
     gene            1232..1885
                     /locus_tag="EYO86_04395"
     CDS             1232..1885
                     /locus_tag="EYO86_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIC42305.1"
                     /translation="MSNYIKILPNYLIKRYKAWKATVYEEKKNWYNKIASEGQNPRVM
                     VISCCDSRIHATSIFGADTGEFFIHRNIANLVPPYKPDGDHHGTSAAVEYAIKTLQVS
                     HIIILGHSHCGGIMSGYKLFNDDQISNESIFVDKWLNILKPAYENISNNGNQFNEGCI
                     VDLEKESIKFSINNLTDFPFIKSALIKKELVLHGLWHDIGSGTLEALDPISMNFKKI"
     gene            complement(1886..>2151)
                     /locus_tag="EYO86_04400"
     CDS             complement(1886..>2151)
                     /locus_tag="EYO86_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42306.1"
                     /translation="IGVIIVGLGSGFYLISNLGPGSRDGLMTGLQKKTNLPIALIRAT
                     IEVSAVVFGFYLGGVVGIGTLVFAFGIGPAVSAGLFFVSRFFK"
BASE COUNT          746 a          349 c          338 g          718 t
ORIGIN      
        1 agaaaataaa aatagacttg gattgtcttt tgggcatatt tccaatgcaa acctcggtga
       61 caaaaatcca ggagtggaaa tcattagcct ttcttaccag gttccttttt agctaaggat
      121 tgcttttttc tgcagattga taaactgtag aaataaagat tctagattat ctatttcttg
      181 atccttaatt tgttgatgaa tctctgtttt taatttttta ctatcaggaa taccaaagaa
      241 aatttgcaat agagggctta ataaacgaaa aatagaatca tttccaatct tgggttttat
      301 gtaattaaaa taatctagaa taattttttt attaagattt ttttcaccct tgaaactaaa
      361 aaataattta tcaactttgc ctaagcaaaa gggattattt tggattaatc gaccaagcat
      421 tacgccatca tgctctttcg acaatgcgta agctttgtct aaactatcaa ttcccccatt
      481 aagtataaat tgaacatgag gatgctcttt ctttattttt tttacaaact cataatttaa
      541 cctaggaatc gagcgattac ctttagggct aataccattt aaaattgcat tgcgagcatg
      601 gacgtatatt atgttaatac cactttggac aatttcatca ataaaagttg aaaagtaatc
      661 gtaatttaaa tcattgccga gaccaatgcg acatttcata gtaacctcaa tatcgctggc
      721 attttgcatg gtttctaaac atgctctcac taattttttg tcttgcataa gacatgcacc
      781 gaaactgcct ttttgaaccg ctttgcttgg gcaaccaaca ttaagattga tttcatcata
      841 gttataagat tttgcaattt ttgaacagag cgccaaatca tcaatatcgg atccacctac
      901 ttgcagtgct ataggatttt gtaaatcatt ttcaataata agatcttttg ttttagagtg
      961 taacattgtt gatgcagcta tcatttcagt aaagagaaat gttttttgag agagaatacg
     1021 ataaagtttg cgtgcatgtg gggttgtata acccatcata ggtgctacgc agaattttcg
     1081 actatatttg tctttgctca tatatctcca acttcatatc aaaaaaaaaa tatttttata
     1141 taaataataa aaatttatgt atagtgaaaa ataagaaatt attacatagc tttagcccct
     1201 gctataaatt ttatgttaga atattattca tatgtctaat tatatcaaaa ttttgccaaa
     1261 ttatttaatc aagagatata aagcttggaa agcaacagtc tatgaagaga aaaaaaattg
     1321 gtataacaaa atagctagtg aaggccaaaa tccacgagtc atggttattt catgttgtga
     1381 ttctcgtata catgcaacat ctatatttgg agctgatacc ggagagtttt ttatacatcg
     1441 caatattgcc aatttagttc caccatacaa acctgacggc gaccatcatg gcacatctgc
     1501 tgctgtggaa tatgcaatta agacattaca agtttcccat atcatcattc ttggtcattc
     1561 tcattgtgga gggattatga gtggttataa attattcaat gatgatcaaa tttcaaatga
     1621 gtcaatattt gtggataaat ggttaaatat tttaaaacct gcatatgaga atattagtaa
     1681 taatggaaat caatttaatg aaggatgcat tgtagattta gaaaaagaaa gtataaaatt
     1741 ttctataaac aatctgaccg attttccttt tatcaaaagt gcacttatta aaaaagaatt
     1801 ggttttgcac ggattatggc atgatattgg atcaggaaca ctggaagcac ttgatccaat
     1861 atccatgaac tttaaaaaaa tttagttatt taaaaaaacg actaacgaaa aataaacctg
     1921 ctgatactgc agggcctatg ccgaatgcaa atacaagagt gcctattcct acgactccac
     1981 caagataaaa accaaaaact actgcgctaa cctcaattgt agcgcgaatc aaggctatag
     2041 gtaagtttgt ttttttttgt aatccggtca tcagtccatc tcttgagcca gggccaagat
     2101 tggaaattaa ataaaaaccg cttccaagcc caacaattat tacaccaata a
//
LOCUS       DTVV01000371            1350 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2083904, whole genome shotgun sequence.
ACCESSION   DTVV01000371 DTVV01000000
VERSION     DTVV01000371.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2083904"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            469..783
                     /locus_tag="EYO86_04405"
     CDS             469..783
                     /locus_tag="EYO86_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42307.1"
                     /translation="MKSLYGNTGTPITLDMGMFKVFYREPLLGGNRLYQAKVATLMDA
                     VASFNLHKGGFDTPLSVERIDMSGEMTHRWNLDGRYSVPRLVGGTVTTDRPRRGRRST
                     RR"
     gene            complement(895..1086)
                     /locus_tag="EYO86_04410"
     CDS             complement(895..1086)
                     /locus_tag="EYO86_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42308.1"
                     /translation="MFDSGSLSYNHIKEDGHPNEKRPNDQSVLGILEGPSIQKGIPTE
                     KILVGISGKGFHIYIYFPH"
BASE COUNT          388 a          298 c          299 g          365 t
ORIGIN      
        1 gagtcagagg aagaccttga tgacttctgc cactatgtgg ctgagcttgt aatagagatt
       61 gagacactag aggagcatat agggcttctg gaggctgata tcaatgtccc tctaatacaa
      121 gtcaattaga gaacccttat acagagaccc tatatagacc cttatgtata agccctatat
      181 gatagcagtg ccttatggtt cctcgaggcc tttacaggtt cctaagtcat tgttatataa
      241 gccttttctt gttgtataaa agcaacacat ggagcccaac tgtccaaacc gtctattcga
      301 cacatatata aaacctttat atatcaatga gttatgaggg tatttgacag aggccccaaa
      361 gtgtggtata atgatgtttc aagaatagag attagccctt actggcgtct atctgattag
      421 actagaatcc aagagattac tgcaaagggc actttttaag agatcaatat gaaatcacta
      481 tatggcaaca ccggaacccc cattactcta gatatgggaa tgtttaaggt gttctatcgc
      541 gagcccctat taggcggcaa tcgcttatat caagctaagg tggccacact gatggacgcc
      601 gttgcttctt ttaacttaca caaaggcggc tttgatacac ctctctccgt agagcgtatt
      661 gatatgtcgg gcgagatgac acaccgttgg aatctcgatg gccgttacag tgttccccgc
      721 ttagtcggtg gcactgtaac aacagatagg ccacgtcgcg gccgtcgcag tactcgcaga
      781 tgatagaatc tattttcctt attctatggg tcatagtgat ccttagagta gtctggtaga
      841 cccttaaagg gaaaacgaca ctagtattgc ccagcccttc aaagggatac aatctcaatg
      901 agggaagtat atatagatat gaaaaccttt tccagaaatt ccgaccagaa ttttttccgt
      961 cggaatacct ttttgaatgg atggaccttc caagatgccg agaacagact ggtcatttgg
     1021 acgcttctcg ttgggatgtc cgtcctcctt tatgtgatta tatgataatg acccagaatc
     1081 aaacaaaaga ccaactggct aaactcttag caatggagga catacacgtc cgccattcgg
     1141 gtaatgctag tactgcttca tttgatgtca aggaccgtgt cctaacccta ccagtttggg
     1201 cggacatgac atccgacatc atagacatgc taacgggtca cgaagttgga cacgccctct
     1261 acaccctacc tgacaagtgg cacgatgcca ttgacgaagg tgtacacaag ggtattctca
     1321 atatcgttga ggatgctcgg atagaaaaga
//
LOCUS       DTVV01000372            1802 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2094533, whole genome shotgun sequence.
ACCESSION   DTVV01000372 DTVV01000000
VERSION     DTVV01000372.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2094533"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="glpK"
                     /locus_tag="EYO86_04415"
     CDS             <1..1131
                     /gene="glpK"
                     /locus_tag="EYO86_04415"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:PRK00047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIC42309.1"
                     /translation="NGLNEEIINKTGLIIDPYFSATKIAWILNNIEDARRLALKDRLA
                     VGTIDTFLLFKLTNGKSFYTDITNASRTSLYNIHDLEWDNSLLEIFDVPINILPEVKD
                     NIFEYGITENNLFGKSIPILSIAGDQQSSSLGQFCLNAGDVKATFGTGCFILMNTGNN
                     IIKSKNKLLTTIAYKIGKKTNYALEGSIFISGAAVQWLRDNLKIIKKAEDTQALASSI
                     KNNNGVYFVPAFTGLGAPYWKPEARGAIYGLSRSSGPAEISRAALESVAYQCADLLDA
                     MRSDGQKPKVMRVDGGMSSNDWLMQFLSNISELTIERTGNKESTSYGIAILTALGSGL
                     ISSLKEFSNNWTVNRKFNPEMSRNEIDKYRNGWQHAIKKTIM"
     gene            1167..1484
                     /locus_tag="EYO86_04420"
     CDS             1167..1484
                     /locus_tag="EYO86_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42310.1"
                     /translation="MNDLIIENGTIIDGTGSPRKIANISVKDGKITEVGNVSGPATRI
                     IDAKDKLVSPGWVDIHTHYDGQATWDSYLTPSSWHGVTTAVFGTVRKRALSKKAWTTS
                     KEI"
BASE COUNT          636 a          295 c          362 g          509 t
ORIGIN      
        1 aatggattaa atgaagaaat tataaacaaa accggtttaa ttatcgatcc ttatttttct
       61 gcaacaaaaa tagcatggat actaaacaac attgaagatg cgagaagatt ggctttgaaa
      121 gacaggctag cagtaggaac cattgacaca tttttattat ttaaattaac taatggtaaa
      181 agcttttata ctgacataac aaatgcatcc cgaaccagtc tttataatat tcacgatctt
      241 gagtgggata attcattatt ggagattttt gacgttccaa taaatatatt acctgaagta
      301 aaagataata tttttgagta tggaattact gaaaacaatt tattcggaaa aagtataccg
      361 atcttgagca tagctggaga ccaacagtca tcaagtttag ggcaattttg tttaaatgcg
      421 ggagacgtca aggcaacttt tggtacagga tgttttatct taatgaatac tggaaataat
      481 attattaaaa gtaaaaataa attactcaca actattgcat ataaaattgg aaaaaaaaca
      541 aattatgcct tggaaggatc tatttttatt tctggagcag cagtccagtg gttaagagat
      601 aacttgaaaa ttattaaaaa agccgaagac actcaggctc ttgcatcgag tataaaaaat
      661 aataatggtg tatattttgt tcctgcattt accggattag gagcccccta ttggaaacct
      721 gaggctagag gagctatata tggattatca agatcttcag ggcctgctga aatctctcga
      781 gcagcattag aatcagtagc ttatcagtgt gccgatcttt tggacgcaat gagatctgac
      841 ggacaaaaac ctaaagtaat gcgagtggac gggggtatga gttcaaatga ttggttaatg
      901 caatttctat caaatataag cgaactaacc attgaaagaa cgggcaacaa agaatccact
      961 tcttatggaa ttgctatatt aacggctttg ggatcaggtt taattagctc tttaaaagaa
     1021 ttttcaaaca attggacagt gaacagaaag tttaatcctg aaatgagtag aaatgaaata
     1081 gataaatata gaaatggttg gcaacacgct attaaaaaaa ctataatgta gtccacaaaa
     1141 aattataaaa ataatgttta aaaaagatga acgatttaat tatagaaaat ggaactatta
     1201 tcgacggtac tggttcacct agaaagatag caaatatatc tgtaaaagat ggtaaaatta
     1261 ctgaagttgg taatgtttca ggtccagcaa caagaattat agacgctaaa gataagcttg
     1321 tatcaccagg ttgggttgat attcataccc attacgacgg acaagctact tgggattcat
     1381 atttaactcc ttcatcctgg catggagtta ctactgcagt ttttggaacg gttcgcaaaa
     1441 gagctttatc gaaaaaagca tggaccactt ccaaagaaat atgaaggcgc tatgtggacc
     1501 accctgaagg acctgatcca cgaaggggga ctggatttaa aggacgaggg aagagtattt
     1561 gagagttact cgttttgcgt aagttttcct catgacgacg acaccaagga gcccccgact
     1621 tcaactgaga tagacgctca acacatgcga atgaaccgcc tgaaagaact atatctccaa
     1681 tagtgtgcgc tagtttcttt ccgaaggcct gaattggaat ctagctcggc catatgctgt
     1741 ttcgttgcac ttcggtcata acccctttct acacatgcaa acgtggggtt gcgcacaaag
     1801 cg
//
LOCUS       DTVV01000373            1500 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2095071, whole genome shotgun sequence.
ACCESSION   DTVV01000373 DTVV01000000
VERSION     DTVV01000373.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2095071"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            245..925
                     /locus_tag="EYO86_04425"
     CDS             245..925
                     /locus_tag="EYO86_04425"
                     /inference="COORDINATES: protein motif:HMM:PF02230.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42311.1"
                     /translation="MDDAKILTGPSFLPHDKPKKLIFMLHGYGDTADNFIHIAEVLRQ
                     PVWQANYFALNAPFLIPNYSMGRQWFELYPNGVYISEAGPNEIKIIRSQVKLVVQQIE
                     QTIIKQKNKYNLPFRDCLLLGFSQGGIMTFEFGNFFQDQLAGLVVMSGRIMEQNEITN
                     QYVLQTPIFISHGDQDDVLPIKNFFKSCEYLEKNKCIFEKHLLAGDTHIISPKAIDLL
                     QKFIKKNL"
     gene            990..>1500
                     /locus_tag="EYO86_04430"
     CDS             990..>1500
                     /locus_tag="EYO86_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIC42312.1"
                     /translation="MSTVGNPALVLNADFRPLSYYPLSLCSWQNAIKSVFLERVFIIE
                     NYDQEVHSPTTTFRLPSVIALKDYVMLQRKPAFTRFNVFLRDNFTCQYCHDHFSANEL
                     TFDHLVPRYLNGKTTWNNVVSACTSCNLKKGRRLLKFVDMKLFKEPMRPSAIQLQNNG
                     RNFPPNFLHE"
BASE COUNT          485 a          236 c          250 g          529 t
ORIGIN      
        1 gcttcagctt tcattttaag tttaagctct ttttacattt tgcggtctcc cgtctttgat
       61 ttatgattga aatgtatacc catgccatcc ttaacaaggc ttatactaaa aaaaaaaaat
      121 ttttctatag tgtattaaaa aaacgccaaa tttctagcta actatgcaat aattattaaa
      181 aaaacctaca cttttagtat agtaatgctc atactctgat ctaataatca tcagttttat
      241 atttatggat gatgcaaaaa tattaaccgg gccttcattt ttgccgcatg ataaacctaa
      301 aaaattaatt tttatgctgc acggatatgg agatacagca gacaatttta tccatatcgc
      361 tgaagtatta cgccagcctg tctggcaagc aaattatttt gctttgaatg caccttttct
      421 tattcccaat tattcaatgg gccggcagtg gtttgagtta tatccaaatg gtgtgtacat
      481 aagtgaggcc ggtcctaatg aaattaaaat aattcgttca caagttaaac ttgttgtgca
      541 acaaattgag caaactatta ttaaacaaaa aaataaatat aatttgccat ttcgtgattg
      601 tttattgcta ggtttttctc aaggagggat aatgacattt gagtttggta atttttttca
      661 ggatcaattg gctgggttgg tagttatgtc aggaagaatt atggagcaga atgaaattac
      721 gaaccaatat gtattgcaaa cacccatatt catttctcat ggtgatcagg atgatgtctt
      781 acccattaag aattttttta aatcttgtga atatttagag aaaaataaat gtatttttga
      841 aaagcactta ttggcaggtg atactcatat aatttctcct aaagcaatag atttattgca
      901 aaaatttatt aaaaaaaatc tatgatgtaa gcgaattatc atattatata tttagtaata
      961 aaatatgact ttgaaaagta agaattaaca tgagtacagt tgggaatcct gctttggtgt
     1021 tgaacgcgga ttttcgccct ttaagctatt atcctctttc tttatgctca tggcagaatg
     1081 ctataaaatc tgtttttttg gagcgtgttt ttattattga gaattatgat caagaggtac
     1141 attcacctac aacaactttt cgtttaccga gtgttattgc gttaaaagat tatgtgatgc
     1201 tccaacgcaa accggcgttc acacgtttta atgttttttt gcgcgataat tttacgtgcc
     1261 aatactgtca cgatcatttt tccgcaaacg aattaacttt tgaccacctt gtaccgcggt
     1321 atctaaatgg aaaaacaacg tggaacaatg tagtatctgc ttgtacaagt tgtaatttaa
     1381 aaaaaggtcg gcgtttgctt aagtttgttg atatgaaact atttaaagaa ccaatgaggc
     1441 cttctgcaat tcagttgcaa aataatggtc gtaatttccc tcccaacttt ttacatgaaa
//
LOCUS       DTVV01000374            1364 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2095941, whole genome shotgun sequence.
ACCESSION   DTVV01000374 DTVV01000000
VERSION     DTVV01000374.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2095941"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="EYO86_04435"
     CDS             complement(<1..647)
                     /locus_tag="EYO86_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIC42313.1"
                     /translation="MTKPIIGITLDNEEPGHYSKFPWYAVRQNYLHSVEQLGAIPFPL
                     FHSAHSAKDIFSLIDGLVITGGNFDIDPQMYGKDEDGSRIIKNNRTIFETTICQMCLD
                     TNKPILGICGGQQLLNVVCSGTLIQDIASSNVNSLKHEQLNPRNQTSHSVNIKQKTKL
                     YNIVKKQKIQVNSAHHQAIDKLGNKLIISGYADDGIIEAIEHSQHDWCVGVQWHP"
     gene            712..1143
                     /locus_tag="EYO86_04440"
     CDS             712..1143
                     /locus_tag="EYO86_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011256369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIC42314.1"
                     /translation="MKKALEQANKALLADEIPIGAVLVDNNNNKIISESYNLINANHN
                     ATQHAEMIVIHKACQKRQSKFLSNTSLFVTLEPCAMCAAAISEVHIDKIYFGAYDEKK
                     GGLESMMKIYNQHHFFVPEVYGGMNELECSLLLKNFFLDQR"
     gene            1148..>1364
                     /locus_tag="EYO86_04445"
     CDS             1148..>1364
                     /locus_tag="EYO86_04445"
                     /inference="COORDINATES: protein motif:HMM:PF13742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIC42315.1"
                     /translation="MNELINIPEYQVSQFNRLFRDVIEKNFSYVRIKGEISEIKRATR
                     GQLYLTLKDDESILSGVIWDNKKRDLQF"
BASE COUNT          449 a          206 c          239 g          470 t
ORIGIN      
        1 tcaggatgcc attgaacacc aacgcaccaa tcatgttgtg agtgttcaat tgcttcaatg
       61 atgccatcat cagcataacc acttatgatt agtttattac caagtttgtc tattgcttga
      121 tgatgcgcac tatttacttg gatcttttgt tttttaacaa tgttatagag ttttgttttt
      181 tgtttaatat taacagaatg acttgtttga ttgcgaggat ttagctgttc atgtttcaag
      241 gaattaacat tactagaagc tatatcctgt ataagtgtac cactgcaaac aacattaagg
      301 agctgttgtc ccccgcaaat tccaagtata ggtttgtttg tatcaagaca catttggcat
      361 atagttgttt caaaaatagt gcgattattt tttataattc gcgacccatc ttcatctttt
      421 ccgtacattt gtggatcaat atcaaagttt cctcctgtga tgactaatcc atcgattaaa
      481 gaaaaaatat ctttagcact atgtgcacta tggaaaagtg ggaaaggtat tgcccctaat
      541 tgctctacag aatgtaaata attctgtcgt actgcgtacc aaggaaactt agaatagtga
      601 cctggttcct cattatccaa agtgatgcca ataataggtt ttgtcattgt aatgtcattg
      661 tattattata atttacatca ctaattgact actatgaaca ttaaatattt tatgaaaaag
      721 gcattggaac aagctaataa ggctttatta gctgatgaaa ttccaattgg agcagtttta
      781 gttgataata ataacaataa aatcataagt gagtcctata atttgattaa tgctaatcat
      841 aatgccactc aacatgcaga aatgatcgtt attcataaag cttgtcaaaa acgccagagt
      901 aagtttttat caaacacttc tttattcgtt acgttagagc cttgtgcaat gtgcgctgca
      961 gcaattagcg aagtgcatat tgataaaatt tattttggcg catacgatga aaaaaaaggt
     1021 ggtttggaga gcatgatgaa aatttacaat caacaccatt tttttgttcc tgaggtttat
     1081 ggtggaatga atgaactgga atgctcttta ctattaaaaa attttttctt ggatcagcgt
     1141 tgaatcaatg aatgaactaa ttaatattcc cgagtatcaa gtttcacaat ttaatcgtct
     1201 atttcgagat gtcatagaga aaaattttag ttatgtgcgt attaaaggag aaatatccga
     1261 aattaaaaga gctacaagag gtcagttata tttaacctta aaagacgatg aatctatttt
     1321 aagtggcgtg atttgggata ataaaaaaag agatttacag ttca
//
LOCUS       DTVV01000375            2635 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2103437, whole genome shotgun sequence.
ACCESSION   DTVV01000375 DTVV01000000
VERSION     DTVV01000375.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2103437"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="EYO86_04450"
     CDS             complement(<1..352)
                     /locus_tag="EYO86_04450"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974690.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIC42316.1"
                     /translation="MKFSSFFIPTLKNIPAEAEIISHQLMIRAGMIKQASAGIYSWLP
                     LGLKVLKKIEHIVRDEQNKAGAIEVLMPTIQSADLWIKSGRYEDYGKEMLRIQDRSGR
                     EMLYGPTNEELITDI"
     gene            complement(455..2128)
                     /locus_tag="EYO86_04455"
     CDS             complement(455..2128)
                     /locus_tag="EYO86_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIC42317.1"
                     /translation="MLNSNSLFKIPTKGIYFMSLGGIGEIGANCYLYCCDDKWLMIDL
                     GLTFADEKFPGIDLLVPKIDFIDSITDKLEAIIISHGHEDHAGAVAFFADKIKCPVYA
                     TGFARSLIENRLKEFNSMDKIQFISIDPIKNIHLKNFDLEFIATSHSIPEPYSIIINT
                     KYGKLLHTADWKIDENPTLGANFDTNSFKKIGSEGLLALIGDSTNADIPGYSQSELEV
                     REELIKLFSRYHQRIVITCFSSNIARMESIAIAAKKNNRKVALVGRSMKKTIEAAINN
                     NIIEDHPNFISEEEASFIPRENLVVICTGSQGEKRSALYRIAYNSHKHIHLENEDLVI
                     FSSRDIPGNEKSVNNLKNLLVRQKVEVITSDDDLVHVSGHGYAEEIKKMYDWTRPYIA
                     IPVHGEAKHLVAHAKLAQASQVPLTKILENGKCIKLAPNDPEIYGFVKTGKMIVEGNN
                     LYDSESNFIKDRRRYSFEGIILISIIIQKDFSIHKDIRISSNGLPEFDIIKLTTDFKE
                     NFIYEYLKLNEEKKSSDQFVGKLIKKSIRLSIKSHLDKKPEVNFHIFRL"
     gene            complement(2121..>2635)
                     /locus_tag="EYO86_04460"
     CDS             complement(2121..>2635)
                     /locus_tag="EYO86_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIC42318.1"
                     /translation="VLNININNIPFDVQFNYESKELGSDRIANTLAAIQKYGKNCLII
                     DFGTATTFDVIKNNKYKGGVIAPGIAISHDALVRYASKLKKIPIIRIKTLIGKNTKDS
                     MQSGFYWGYISLINGVIDKIIKQQRFKPAIILTGGLANIFKKEISKKTEHEPNLTLNG
                     LYLIGKIKYA"
BASE COUNT          838 a          450 c          349 g          998 t
ORIGIN      
        1 aaatatcggt tattaattct tcattagtcg ggccatatag catttctcga ccactgcgat
       61 cttgtattct aagcatttct tttccataat cctcatagcg accagatttg atccacaaat
      121 ctgcagattg aatagtcggc attaagacct ctattgctcc agctttattt tgctcatccc
      181 gaactatgtg ttcaattttt tttaacactt ttaatccaag aggaagccaa gaatatatgc
      241 ccgcacttgc ctgttttatc atccctgctc taatcattaa ttgatgtgaa ataatttccg
      301 cttctgccgg tatattttta agggtgggga taaaaaaact agaaaatttc attatttaaa
      361 aatatactta tatcaattta ataaagattc agattaacat aattaacaaa tattcatcag
      421 tagctacaat aactttttga ctgatatatg tcatttatag tcgaaaaata tggaaattta
      481 cttcgggttt cttatctaaa tgacttttaa ttgacagcct aattgatttt ttaatcaatt
      541 taccaacgaa ttgatcggat gacttttttt cttcatttaa tttaaggtat tcgtatataa
      601 aattttcttt aaaatcagta gttaacttta tgatgtcaaa ttctggtaaa ccattagatg
      661 atattctaat atccttatgg attgaaaaat ctttctgaat tattattgat attaaaataa
      721 ttccttcaaa tgagtaacgt cttcgatctt taataaaatt cgattctgaa tcataaagat
      781 tgttaccttc tactatcatt tttcctgtct taacaaaacc atatatctca ggatcatttg
      841 gagctaattt aatacatttt ccattttcta aaatctttgt aagcggtacc tgactcgcct
      901 gagccaattt tgcatgagct acaagatgct tggcctcacc atgaaccggt attgcaatat
      961 atggccgtgt ccaatcatac atttttttaa tttcttcagc atagccatgt ccggaaacat
     1021 gaaccaaatc atcatctgaa gtaatgactt ctactttttg tctaaccaat aaattcttta
     1081 aattatttac tgatttctcg tttccaggaa tatctcgcga actaaaaatt accagatctt
     1141 cattttctag atgtatatgt ttgtgcgagt tgtatgcaat acgataaagt gcagatcttt
     1201 tttctccttg actacctgta catatcacta caagattttc tctaggaatg aaggaagctt
     1261 cttcttcaga aataaaatta gggtgatcct caataatgtt attattaatg gcagcctcta
     1321 ttgtcttttt catgcttctt ccaactaaag caaccttacg gttatttttt tttgcagcaa
     1381 ttgctatact ttccattctg gcgatatttg aagaaaaaca agttataact attcgttgat
     1441 gatatcgtga aaatagtttt attaattctt cacgaacttc taattcagac tgagaatatc
     1501 caggaatgtc agcattagtt gaatcaccta taagcgcaag caaaccttca ctaccgattt
     1561 ttttgaaaga atttgtatca aagttagctc ctaaagttgg attttcatct attttccaat
     1621 ctgctgtatg aagcagtttt ccgtactttg tattaataat aatagaataa ggttctggga
     1681 ttgaatgaga agtggcaata aattctaaat caaaattttt taaatgtata ttttttattg
     1741 ggtcaatgga aatgaattgt attttatcca ttgaattaaa ttctttgagt ctattctcta
     1801 ttaatgatct tgcaaaacct gttgcataaa caggacattt aattttatct gcaaaaaaag
     1861 ccactgctcc tgcatgatct tcatgaccgt ggcttataat aatagcttct aatttatcag
     1921 taattgaatc tataaaatct atttttggaa ctaataaatc aatgcctgga aatttttcat
     1981 cagcaaatgt caatcccaaa tcgatcatta accatttatc atcacagcaa tacaagtaac
     2041 aattcgcccc aatttctcct ataccgccta aagacatgaa ataaatacct ttggttggaa
     2101 ttttaaataa agaattgcta ttaagcatat tttattttgc caataagata caagccattt
     2161 aaagtcaaat tcggttcatg ttcagttttt tttgatattt cctttttgaa aatattagct
     2221 aagcccccag ttaaaattat tgcgggttta aatcgctgtt gcttaataat tttatctatt
     2281 accccgttta ttagacttat atatccccaa taaaaaccac tttgcatcga atctttagta
     2341 tttttaccta ttaaagtttt aattcttatt attggaattt tttttaattt ggaagcatat
     2401 ctaaccagtg catcatgcga tatagcaata ccaggtgcaa taacgcctcc tttatattta
     2461 ttatttttta ttacatcaaa tgtggttgct gtaccaaaat caataatcaa acaatttttt
     2521 ccatattttt gtatagctgc taatgtgttt gctattctat cactgcctaa ttctttggat
     2581 tcatagttaa attgcacatc aaaagggata ttgttaatat taatatttaa aactt
//
LOCUS       DTVV01000376            2147 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2108174, whole genome shotgun sequence.
ACCESSION   DTVV01000376 DTVV01000000
VERSION     DTVV01000376.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2108174"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(370..>2147)
                     /locus_tag="EYO86_04465"
     CDS             complement(370..>2147)
                     /locus_tag="EYO86_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIC42319.1"
                     /translation="TFIKIIPSVYKISESINKKQGNISESSKILSSESDVSVITLKGF
                     IGSYAKLINFLLNHPAKVIVSAILILVGVSFAYTKIGSGIEFFPEVEPELAKIVVYAR
                     GNLSAKEKRDYVSRVENIILKIQSKNNEFKNIYALSGNISDQSEASEDFIGSISLEYN
                     DWDKRRKSKVILNEILNKTKTIKGIKVESREQEGGPPTGKPINIKLTSPNKSLLLSES
                     YRLKEFIDSYPELINIEDNFPAPGIEWEINVNRKQAAKFGANISTIGNVIKLATNGIK
                     LGEYRPDDSNDSIPLYLRYPSEGRTLDIIQNLRVNTAVGLVPIANFVEIIANNRTGNI
                     VRVNSKNAINIQADIEVGVYADAKVKEMEYVLGINKSPPSFRGKPIENLKKFNLDPRI
                     KVQLIGENQDQKEAQDFLSKAFAVALFMMLMILLLQFNSFYSSFLILFSVVMSTTGVF
                     IGLMITGQAFSIVMTGVGVIALAGIVVNNNIILIDTFDFLKNKMPTVREAIIKTGAQR
                     IRPVLLTTFTTVLGLLPMVTMTNVDFVTREITRGSPDTQWWVQLSTAIVFGLIFATIL
                     TLIVTPSALMLRENIKTWYQNKINS"
BASE COUNT          715 a          344 c          282 g          806 t
ORIGIN      
        1 ttacagctga tatttattta acatctaaac ccattgaccc tagcatgtac tttgtagggg
       61 tttcttcccc tgcagcagca ggtttaagtt tattgccttt atttatattt tttgaatttg
      121 atttttcttt tattcaaaat gaatatctaa atttatgtaa tttgagtatt attggctttt
      181 taatggttag caaaatacca acaatgtctt tgaaaaatat taatttttca aaaaaaacaa
      241 gaccctggat cttattatta attgcgataa tttgtattgg tttaatttct aatttatggt
      301 taacattaat attcataaca atcacatata tagcttcaat tttttatact gtttttatta
      361 atttaaggac taagaattaa ttttattttg ataccaagtc ttaatatttt ctctaagcat
      421 caacgctgaa ggagtaacaa ttaaagttaa tattgttgca aatattaatc caaaaacaat
      481 tgctgtagat aactgtaccc accattgtgt atctggagac ccgcgagtaa tttctcttgt
      541 aacaaaatca acatttgtca tagtaaccat aggaagcaaa ccaagcactg tagtaaaagt
      601 agtaagaaga acaggtctta ttctttgagc tcctgtttta ataatagctt ctctaacagt
      661 aggcatttta ttttttagaa aatcaaaagt atcaattaat atgatattat tatttacaac
      721 tattccagca agtgcaatta cgcctacacc agtcattaca atactgaatg cttgaccagt
      781 aatcattaaa ccaataaaca caccagtagt agacataacg actgaaaata aaattaaaaa
      841 actactatag aagctattga actgtagtaa tagtatcatc aacatcataa ataaagctac
      901 agcaaaagct tttgataaaa aatcttgtgc ttctttttga tcttggtttt caccgatcag
      961 ttgtacttta attcttgggt ctaaattaaa cttttttaaa ttctcaattg gtttgcccct
     1021 gaatgaagga ggggacttat ttattcctaa aacatattcc atctctttaa cttttgcatc
     1081 agcataaaca ccaacctcta tatcagcttg tatattaata gcatttttag aatttaccct
     1141 aactatatta cctgttctgt tatttgcgat tatttcaaca aaattagcaa ttggcactaa
     1201 tccaacagct gtattgactc gtaagttttg aattatatcc aaagttcttc cctctgatgg
     1261 atatcgaaga tacaaaggta tgctatcatt agagtcatct ggtctatatt caccaagctt
     1321 aataccattg gtagcaagtt tgatcacatt gcctatagta gatatattgg caccaaattt
     1381 agcagcttgt ttacgattaa catttatttc ccactcaata ccaggagcag gaaaattatc
     1441 ttcaatgtta attaattcag gataggagtc tataaattct tttaatctat aagattcact
     1501 taaaagtaga gatttgttag gtgaagtcag tttgatattt attggttttc cagtgggtgg
     1561 tccaccttct tgttcacgac tttcaacttt aataccttta atagtttttg tcttatttaa
     1621 aatttcatta agtattactt tagattttct tcttttatcc caatcattgt attctaaact
     1681 aatagagcca ataaaatctt cagatgcttc agattgatct gatatatttc cacttaaggc
     1741 atatatattt ttaaattcat tattcttaga ctgtattttt aaaataatat tttctactct
     1801 agaaacatag tcgcgttttt cttttgcaga aagattacca cgagcatata caacaatttt
     1861 tgcaagttct ggttctactt ctggaaaaaa ttcaatccca gatcctattt ttgtataagc
     1921 aaagctaaca ccgaccaata ttaatattgc tgatacaatt acttttgctg gatggtttaa
     1981 taaaaaattg attagctttg cataggaacc tataaaacct tttaaagtaa tgacagacac
     2041 atcactttca gaagataaaa ttttcgatga ctcacttatg tttccttgtt ttttatttat
     2101 tgattcactg atcttataaa ctgatggtat aatttttatg aaagtaa
//
LOCUS       DTVV01000377            1928 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2112108, whole genome shotgun sequence.
ACCESSION   DTVV01000377 DTVV01000000
VERSION     DTVV01000377.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2112108"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="EYO86_04470"
     CDS             <1..197
                     /locus_tag="EYO86_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42320.1"
                     /translation="MPMVGHVTSSYYSPNLGRSFALALVKGGLKKKGNKLLAPMPDKT
                     IEVEITDPVFIDPSNERLST"
     gene            197..775
                     /locus_tag="EYO86_04475"
     CDS             197..775
                     /locus_tag="EYO86_04475"
                     /inference="COORDINATES: protein motif:HMM:PF04268.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42321.1"
                     /translation="MLKRESVLKDQGIQVYNGIEFEELSHIPKINLRGQSDNKDFMSS
                     SGTILDILLPTEPNISNVTVNAKVIWLGPNEWLVEINNENKFKEIFSKLQSTLNPQNT
                     AVTDLTENRTIIKVSGHNLYTLLAKFMVIDLDTVLQKETAVAQTLFIKVPILLVRNHK
                     YSEKPSIDIHTNRSHAQYIYKILVDGSHNLDF"
     gene            750..1223
                     /locus_tag="EYO86_04480"
     CDS             750..1223
                     /locus_tag="EYO86_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIC42322.1"
                     /translation="MAAITWIFSILFIFSFAPNLASKTITGNAKVVDGDTIHIGLNKI
                     RLHGIDAPETNQTCEKKNVAWLCGKESTQALINFINNKKVICNVKGTDQFKRYISVCF
                     VNKLDMNEFMVKNGWAIAYRFYSKDYTESEDKAKKDKAGIWQGSFQEPYLFRKNN"
BASE COUNT          674 a          289 c          267 g          698 t
ORIGIN      
        1 ctatgccaat ggttggccat gttacatcta gttattattc accaaatctc ggacgttctt
       61 ttgctcttgc tcttgttaaa ggtggattaa agaaaaaagg aaacaagtta cttgcgccaa
      121 tgccagataa aacaattgaa gtggaaataa ctgatcccgt atttattgat ccttccaatg
      181 aaaggttaag tacgtaatgc taaaaagaga aagtgtatta aaagatcaag gaatacaagt
      241 ttacaatggc attgaatttg aagagttatc tcatatccct aaaatcaatc ttcgaggaca
      301 atccgataat aaagatttta tgtcaagttc tggaacaatt ctagatattc tcctaccaac
      361 ggaacctaac attagtaatg ttactgttaa tgctaaagta atctggcttg gtccaaatga
      421 atggctagtt gaaattaaca atgaaaacaa gtttaaggaa attttctcaa aacttcaatc
      481 aacactcaat cctcaaaata cagctgtaac tgatttaaca gaaaaccgaa caattataaa
      541 agttagtggt cataatcttt atactttatt agcaaaattt atggtcatag atttagatac
      601 agtccttcaa aaagaaaccg ctgtagctca aacattgttt ataaaggtac caatcttgtt
      661 ggtgcgaaat cataaataca gtgaaaagcc cagtattgac atacatacta accgctcaca
      721 cgcccaatat atttataaaa ttcttgtaga tggcagccat aacctggatt tttagcattc
      781 tatttatatt tagctttgcg ccaaatttag cttccaaaac aataacagga aatgcaaaag
      841 ttgtcgatgg tgacacaatc catattggat taaataaaat aagacttcac ggaatcgatg
      901 cgcctgaaac taatcaaact tgtgaaaaaa aaaatgtagc atggctgtgt ggtaaagaat
      961 ctacacaagc tctaattaat tttattaata ataaaaaagt tatttgcaat gttaaaggca
     1021 ctgatcaatt taaacgctat atatctgtgt gctttgttaa caaattggat atgaatgaat
     1081 ttatggtgaa gaatggatgg gcaattgctt accgctttta ctcgaaagat tatacagaat
     1141 ctgaagataa agcaaaaaaa gataaagccg gcatctggca aggttctttt caggaacctt
     1201 atttgttcag aaaaaataac taaccccaaa aaaatataaa ttgttacacg aaaaattaca
     1261 gcattgatcc aaattaaaaa atatttaatt ttctcctatt attaacctta tttaaaaaaa
     1321 actaataacc aattgaatta atgatgagat gtatcgtcca attttttgat aagagtttct
     1381 tgctccttaa tctgttgttc caaactctgt ataaggattt ttaacttttt atttccatta
     1441 actaactttt tattttcatc agttaacttc atatttttat taaataaact tttgtactct
     1501 tttgttaaaa gttgtttttc aataacattt tctttttgag atttacttga tacttcattg
     1561 tttttttttg tagtttcaat ttcctgattt aattttttta tcttttgttg taactctaat
     1621 tggatcttat taattttttc cttagaattt tcaatttcca cattttgatt aattgtatct
     1681 gcaagttttg tttcaataga tttacttaat ttactatttt caactgttag aatttcaatc
     1741 ttttttttca ttttattgat ttgttgatca cgaaaaataa ttttttgttc tttttgaaaa
     1801 atttgtaatt ccaactctgc aagatctatt tttaattctg gagaaatagt ctcttcttca
     1861 ctaggaagta atgtatctaa tgatttattt tcatctgatt gtatttttga aaaaactaaa
     1921 tacatcaa
//
LOCUS       DTVV01000378            2222 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2117568, whole genome shotgun sequence.
ACCESSION   DTVV01000378 DTVV01000000
VERSION     DTVV01000378.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2117568"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2222)
                     /locus_tag="EYO86_04485"
     CDS             complement(<1..>2222)
                     /locus_tag="EYO86_04485"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIC42323.1"
                     /translation="GDMIHIEDIQDQVELALMRDEHHKVARAYVLYREQRAASRYHTK
                     KLKEQVGVKVSSMMVTKRDGNKEPVSLDKITNRVSVLSTGLAIDPITVAQKAIPGLFN
                     DITSTEIDNYLAETSAALTVEHPDYSNLAARIKANALHKETPGFIIATKNLYEDGLLK
                     EEYYNKVIANSEAIESIIDYDRDYSFDYFALTTLTRAYLLKYDNKTIERPQDLWMRVA
                     LTVSCDNFDFNKVKETYNTLSSGMYTHATPTLFNSGLKMQQLSSCFLISMEDDSIEGI
                     FNTIKDCALISKTAGGIGIHAHNIRASGSRIKSTNGKSNGLIPFLKIFNETAKSVDQG
                     GGKRKGSFAVYLEPWHADIEKFLELRKNTGAEEFRARDLFYALWIPNLFMKRVENDED
                     WTLMSEHECPGLSDHYGEAFERLYAKYEKDLPHLKKIKARNLMSKIIEAQIETGQPYM
                     LYKDAINEKSNQKNIGVIKSSNLCAEIVEYSNKSETAVCNLASIALPKFIDKKNKKYD
                     YKKLYQTAKLATKNLDRVIDINFYPTDKTSTSNNLHRPIGLGVQGLSDVFFILSIPHE
                     SEEAGKINKLIFETIYFGCLEASMELAKEKGTYSSFEGSPLSDGKFQFDLWNTKPSNM
                     WDWETLRTKIKKHGVRNSLTTACMPTASTGIILGNTETFQIQTSNIYKRQTLSGEFLL
                     VNRHLVKELSRRNLWNKEIRDNIVLQNGSVQNIPNFPDDLKEIYKTVWETSQRVVIDM
                     A"
BASE COUNT          660 a          381 c          354 g          827 t
ORIGIN      
        1 gccatgtcta tgacaacacg ttgtgatgtt tcccacacgg ttttatatat ttcctttaaa
       61 tcatcaggaa agtttggaat attttgtaca gatccatttt gtaaaacaat gttgtccctt
      121 atttctttat tccatagatt tcttctagat agctctttaa ctagatgtcg attaaccaat
      181 aaaaattcac cagataaagt ttgtcttttg tatatgttgg atgtttgaat ttggaatgtt
      241 tccgtattac ctaaaataat tccagtggaa gctgtaggca tacatgctgt tgtaagtgaa
      301 ttgcgtaccc catgtttttt tatttttgtt cttaacgttt cccaatccca catattggag
      361 ggtttagtgt tccacaaatc aaattggaat tttccatcgg atagtggaga accttcaaat
      421 gatgaataag tgcccttttc ttttgccaat tccatagaag cctctaaaca accaaaatat
      481 attgtttcaa atattaattt attaattttt ccagcctctt cactttcatg aggaattgat
      541 agtataaaaa atacatctga tagaccctga acaccaaggc caattggacg atgtaaatta
      601 tttgatgtac ttgttttatc ggttggataa aaattgatat caatgactcg atcaaggttt
      661 tttgttgcta gttttgcggt ttgataaagt tttttgtagt catatttttt atttttttta
      721 tcaataaatt ttggtagagc aatagaagct aaattgcata cagcagtttc acttttattc
      781 gaatactcaa caatctcagc acataaatta gatgatttaa taactccaat atttttttga
      841 ttagattttt cattaatagc atctttataa agcatataag gttgcccagt ttcaatctgc
      901 gcttcaatta ttttagacat taagtttcta gctttaattt tttttaaatg aggcagatct
      961 ttttcatatt ttgcatacaa tctttcgaaa gcttcgccat aatgatctga aagtcctgga
     1021 cattcatgtt cactcataag tgtccaatct tcgtcatttt cgactcgttt catgaaaaga
     1081 ttaggtatcc acaaagcata aaataaatct cttgctctga actcttctgc acccgtattt
     1141 ttcctcaatt ctagaaattt ttcgatatcg gcatgccaag gttcaagata aacggcgaac
     1201 gaccctttcc ttttaccacc tccttgatca acagatttag ctgtctcatt aaatattttt
     1261 agaaaaggta ttaatccatt ggattttcca ttagttgatt ttattcgact tccgctagcc
     1321 cgaatattat gggcatgtat acctatgcca cctgcagttt tagagataag ggcacaatct
     1381 ttaattgtgt taaaaatacc ttctattgag tcatcttcca tgcttattaa gaaacaggat
     1441 gataattgtt gcattttaag tccactattg aataatgtag gtgttgcatg ggtgtacata
     1501 ccgcttgata gagtattata tgtttcttta actttattaa aatcaaagtt atcacaagaa
     1561 acagtaagag cgacacgcat ccaaagatcc tgaggtcttt caatcgtttt attatcatat
     1621 ttgagaagat aagctctagt tagagtggtt agagcgaaat aatcaaagct gtagtctcta
     1681 tcataatcga ttattgattc aattgcctca gaattagcaa ttactttatt atagtattct
     1741 tcttttaaaa gaccgtcttc gtataaattt ttagttgcaa tgataaaacc aggcgtttcc
     1801 ttatgaaggg cattagcttt tattcttgcg gctaaattag aatagtctgg gtgttcaact
     1861 gttagagcag ctgaagtttc agcaagataa ttatctatct cagtggatgt aatatcatta
     1921 aataaacccg gtattgcttt ttgagcaact gtaattgggt caattgcaag acctgttgaa
     1981 agaaccgaaa ctctattagt tattttatca agagaaactg gttctttgtt gccatctcgt
     2041 ttagtgacca tcatagatga tactttgact ccaacctgtt cttttaattt ttttgtatga
     2101 tatctagaag cagctcgttg ttctctataa agtacgtaag ctctagcaac tttatgatgc
     2161 tcgtcacgca ttaaggctag ttccacttga tcttgaatat cttcaatgtg aatcatatcc
     2221 cc
//
LOCUS       DTVV01000379            1343 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2118134, whole genome shotgun sequence.
ACCESSION   DTVV01000379 DTVV01000000
VERSION     DTVV01000379.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2118134"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            272..1183
                     /gene="argB"
                     /locus_tag="EYO86_04490"
     CDS             272..1183
                     /gene="argB"
                     /locus_tag="EYO86_04490"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIC42324.1"
                     /translation="MKFDFLSESDQAYFIHKASTLVEALPYIREHSGKNIVIKYGGHA
                     MDSGNLAENFSKDIGLLKEVGINPIVVHGGGPQIGKMLKKKKIDSNFVEGLRVTDKET
                     VKVVEEVLANDINKKIVEDINSSGGKAMGFAGNQNNLITAKKLEMAVKDSDSNIEKIL
                     DLGFVGEPISINSSFINDHIVKGIIPVIAPLGIDEKGNTYNINADTAAGAIAGALRAS
                     KLLLLTDIAGILDENKKLISSLSYEEAIKIISKNFIRGGMKPKITTCIKAISQGVKQA
                     TILDGRIPHALILELFTEHGIGTQIYT"
BASE COUNT          512 a          179 c          223 g          429 t
ORIGIN      
        1 caagtttttg ttttaattga ttgcaaagtt ggtgttaaag atgttgatat tgatattttt
       61 gattccataa gcactgccga taaaaaattt tcgattattt taacaaaatc tgataaatgc
      121 gctttaaatt ttattatcga acagcaaaaa tcaatttttt cacttatgag aaattataat
      181 aaacatttta tcaaaattta ctcaaccagt agcaaaaaaa atctaggggt aatcaatata
      241 caaaaagcca tatttaattt atcaacatat catgaaattt gattttttat cagaaagcga
      301 tcaagcatat tttatacata aagcgtcaac attagttgag gcgcttcctt acattcgtga
      361 acattcaggc aaaaatattg taatcaaata tggagggcat gctatggata gtggaaatct
      421 agctgaaaac ttttctaaag atatcggatt attaaaagaa gtaggaatta atccaatagt
      481 tgtgcacggt ggtggtccac aaattggaaa aatgttaaag aaaaaaaaaa ttgatagcaa
      541 ttttgttgaa ggacttcgtg tcaccgataa agagacggtc aaggttgtcg aggaagtgtt
      601 ggccaatgat atcaataaaa aaattgttga agatattaat tcatctggag gcaaagccat
      661 gggttttgct ggcaatcaaa ataacttaat tacagctaaa aaattagaaa tggctgttaa
      721 agatagtgat tctaatattg aaaaaattct tgatttagga tttgttggcg aaccaatttc
      781 aattaattcc tcctttataa atgatcatat tgttaaggga ataattccag tcattgcacc
      841 tttaggtata gatgaaaaag gtaatactta taatattaac gccgataccg ccgctggagc
      901 aattgctggc gccctaaggg ctagtaaact tcttctttta acagatatag ccggaatatt
      961 agatgaaaat aaaaaactta tttcttcatt aagttatgaa gaagcaataa aaattatttc
     1021 aaaaaatttt attcgtgggg gaatgaaacc taaaattact acatgtatca aagcaattag
     1081 tcagggagta aaacaagcaa caattttaga tggaagaatt cctcatgcat taattttaga
     1141 attatttact gagcatggta taggaacgca gatttacact tagttatgcc caccctaaaa
     1201 aataaaatta acacttggat ttttgattta gataatactt tgtactcagc agatagtgga
     1261 atatttcaac aagtacatac tttaatgagt aaatttgttt cagcacactt aaatattgat
     1321 ataaaaaaag caacagaact tca
//
LOCUS       DTVV01000380            1250 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2128436, whole genome shotgun sequence.
ACCESSION   DTVV01000380 DTVV01000000
VERSION     DTVV01000380.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2128436"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="EYO86_04495"
     CDS             complement(<1..214)
                     /locus_tag="EYO86_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HIC42325.1"
                     /translation="MKAEIYSRTNCVFCDKAKMRLSKYNPTVRMLDQDYTRKDFFKKF
                     PNAKTFPQIIINGEHVGGYRELKKWLD"
     gene            complement(214..1203)
                     /locus_tag="EYO86_04500"
     CDS             complement(214..1203)
                     /locus_tag="EYO86_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase"
                     /protein_id="HIC42326.1"
                     /translation="MISKGIKSIFPIKNQEIWDRYKLHIQSFWTPEEVSLQDDLRDLQ
                     ILNEGEKHFIKNVLAFFANSEAMINENLASRFYKEILMPEARCFISMQMLNESIHAEM
                     YALQIEAYVVDQQEKDMLFNAIQNVPCINLKAKWVSKWLSGSQNLLTRLIAFGLVEGL
                     FFAGSFCAIYYFRKRGLLPGLALSNDWIARDEGMHFSFSALMFKILRDKFNNNSLTDT
                     DLLDVNLITGNVAQSEFEEIVREAVSFEKEFVQEALPVDLIGMNANLMCQYIEAVADR
                     IADLFEFERVYNTKNPFDFMRALDVQNVTNFFEKRVSEYQRPTDRVLSFDDVF"
BASE COUNT          402 a          248 c          173 g          427 t
ORIGIN      
        1 gatctagcca ttttttcaat tcgcggtatc caccaacatg ctcaccatta atgatgattt
       61 gaggaaaggt tttggcatta gggaattttt taaagaaatc ttttcttgta tagtcttgat
      121 ctagcatacg cactgtagga ttatatttgc ttaagcgcat tttcgcttta tcacaaaaca
      181 cacagttagt tctagaataa atctcagctt tcattagaaa acatcatcaa aactaagcac
      241 cctatcagta ggtctttgat attctgatac tcttttttca aaaaagtttg ttacattttg
      301 aacatccaaa gctctcataa agtcaaaagg atttttcgta ttatatactc tttcaaattc
      361 aaagagatca gcaattcgat cagcaacagc ttcaatatat tggcacataa gattagcgtt
      421 cattccaatt aaatccactg gtaaagcttc ttgtacaaat tctttttcaa aggatactgc
      481 ctccctgaca atttcttcaa attctgattg tgcgacattc cccgttatta aattgacatc
      541 taacaaatca gtgtccgtaa gagagttatt gttaaattta tcacgaagta ttttgaacat
      601 aagtgccgag aaactgaagt gcattccctc gtctcgagct atccaatcat tggacaaagc
      661 caatcctgga agtaatcctc tttttctaaa ataataaatt gcacaaaaag aacctgcaaa
      721 aaatagacct tccactaaac caaacgcaat caaacgagta agcaagtttt gacttccact
      781 taaccatttt gatacccatt tagccttaag gttaatacat ggaacgtttt gaattgcatt
      841 gaacaacatg tctttttcct gttgatccac aacgtacgct tctatttgca aagcatacat
      901 ttccgcatgt atagattcat taagcatttg catcgatata aaacaacgtg cttccggcat
      961 taatatttct ttataaaatc tgcttgccaa attctcattt atcatagctt ctgaatttgc
     1021 aaaaaaagcc aatacatttt ttataaaatg cttctctccc tcgtttaaaa tctgcaaatc
     1081 tctcaaatcg tcttgaagag aaacttcctc tggagtccag aaggattgaa tgtgtaattt
     1141 atatctatcc catatttctt gatttttgat tgggaagatt gatttaatac cttttgagat
     1201 cattcttatt ctttaatatt taagcactgc aagtcaaaca ttcttccagc
//
LOCUS       DTVV01000381            1085 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2132208, whole genome shotgun sequence.
ACCESSION   DTVV01000381 DTVV01000000
VERSION     DTVV01000381.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2132208"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            382..594
                     /locus_tag="EYO86_04505"
                     /pseudo
     CDS             382..594
                     /locus_tag="EYO86_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001978090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            603..911
                     /locus_tag="EYO86_04510"
     CDS             603..911
                     /locus_tag="EYO86_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42327.1"
                     /translation="MANEFDNYIIKLEKKLENQNSKGENQYAREYIIQRKKETIPDFS
                     QILQLGMTYEEVKQIKGLPKHIDTMNEPRRQFELWTYSTDSTISRLYFENNMLVRIEK
                     "
BASE COUNT          402 a          176 c          175 g          332 t
ORIGIN      
        1 ataagtgctc caattgaagc tgacaagcat ggtgcaatac caccggcagt taaaaatgca
       61 attttcaatt ttaataattc aataatagaa ggatcgaaat atattaaaat tttctgacac
      121 ttattattgc attaatcgtt ctacattgat ctttttcctt aaatttcaaa tgcgtttact
      181 tatcaataat aaaactgtaa aataatatct gatatcctct ggtcaccaga tccatgtgga
      241 gtacgtatag gtacagcaga aatttatcga gttgtagaga atataaacga tagtttagtt
      301 gtatggcagg aatggcatga tgacgagcgc gtaattcttt ttgttcaact tgcggaaaat
      361 atagaattaa caaatgaatc gatttcaaaa ttaaaatcga ctatcaaaaa aacgtgctcg
      421 cctcgctatg tgccatcaat aattttacct attggtgata ttccctaaac aatcaatggg
      481 aaaaaggttg aaatcgcagt taagaagatt ctgaaaggcg aagatgtatt gaatagggat
      541 gcgttggcaa accctaaatc ccttgatcta tacaagcaat atttctgctt ttaaaccctg
      601 aaatggcaaa tgaattcgac aactacatca taaaacttga gaaaaaatta gaaaatcaaa
      661 attcaaaagg tgaaaaccaa tatgcccggg aatatattat tcaaagaaag aaggaaacaa
      721 ttcctgattt ttcacaaatt ctgcagttag gaatgaccta tgaagaggtt aaacaaatta
      781 agggacttcc taaacatatt gatacaatga atgaaccccg ccgtcaattt gaattgtgga
      841 cctactcaac tgattcaacc atatcccggc tctattttga aaataatatg ttggtgagaa
      901 ttgagaagta ggtattttaa aaatataaaa taaaacttca tttattttca gctgcaaata
      961 aacaattcct ctatttaaaa gggtaattgt aacaacatat tacccccctt tcaggtaatg
     1021 ctttattctg cctaaatttc ttgctatgaa aaaattgata ccatttgcca taattacagg
     1081 ggtat
//
LOCUS       DTVV01000382            1088 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2137303, whole genome shotgun sequence.
ACCESSION   DTVV01000382 DTVV01000000
VERSION     DTVV01000382.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2137303"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(88..759)
                     /locus_tag="EYO86_04515"
     CDS             complement(88..759)
                     /locus_tag="EYO86_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIC42328.1"
                     /translation="MSIAIFVLTLDEIIGVNLIMPQVKKEWAEQIVFVDGGSTDGTVE
                     RAKELGFDVIHQHNKGEGNACRVGTDATKSDYVMFFSPDGNDLPEDIPKLIQKSKEGH
                     DIVHISRFGKKSQSDDAGWLDRFGNNMFTFLVNTFFGGKYTDALNGFRILKREIWNDL
                     KTDAQYLNIEQQICIRCAKSNIPIYEIESREPARVGGERIMRPLDVGAQLSYQIIKEF
                     IFWKY"
BASE COUNT          376 a          193 c          131 g          388 t
ORIGIN      
        1 tataatttaa aacctaatga aattaaagaa attacaatta gaaaacaaat aattagtttc
       61 ttattcattc ttttttcagt tattttttta atacttccaa aaaataaact ccttaataat
      121 ttgataactc aattgagcac ccacatctaa aggcctcatt attctctcac caccaaccct
      181 agcaggctct ctactttcaa tttcataaat tggtatattg ctttttgcac atcgtataca
      241 aatttgttgt tcaatgttca aatattgtgc atccgttttc aaatcattcc atatttctcg
      301 cttcaaaatt ctaaaaccat taagtgcatc tgtgtatttt cctccaaaaa aagtatttac
      361 tagaaaagta aacatgttat ttccaaatct gtcaagccat cctgcatcat cactttgtga
      421 tttttttcca aatctagaga tatgaactat atcatggccc tctttagatt tttgaattag
      481 tttaggaata tcttcaggca gatcatttcc gtctgggcta aaaaacataa catagtctga
      541 ttttgttgcg tctgtaccaa ctctacaggc atttccttct cctttattat gctgatgtat
      601 tacatcaaat cctagttctt tggccctttc tacagttcca tcagttgaac caccatcaac
      661 aaaaactatt tgctccgccc actccttttt cacctgtggc ataattagat tcacaccaat
      721 aatttcatct aaagttaaga caaagatggc tatagacata gaaatcgctt tttttgatta
      781 tttaaaattg ttcttactta tcaataagga agaataagtc taattttata ttgccaaatc
      841 taatattaga aatttaccta gtgataaaat tataacaaaa tataattcct tattcttaag
      901 ataattatta tgcgaatggt aaatgtagat aaaaacatca taaccaaatt aagagaatca
      961 ggaatttcag atgaaatgaa aagtatagaa tcatcagatt ggattattta tgagacaata
     1021 gataatgaaa ttataggtgc agctggtata ggcggaatat ttcatacttc aagtatcaac
     1081 attcatga
//
LOCUS       DTVV01000383            2378 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2139836, whole genome shotgun sequence.
ACCESSION   DTVV01000383 DTVV01000000
VERSION     DTVV01000383.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2139836"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="deoC"
                     /locus_tag="EYO86_04520"
     CDS             <1..879
                     /gene="deoC"
                     /locus_tag="EYO86_04520"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014881352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIC42329.1"
                     /translation="AVERRTISLVKRRSIKKDYQAAWLLKAITLIDLTTLSGDDTFGK
                     VDRLCQKALNPLAHNILNVLDIPPNSLTVGAVCVYHHLVHQAQKNLVGKIPVAAVSTG
                     FPAGLSSFFTRKKEISDSIKAGAKEIDIVINRGFVLQNNWQKLYNEVKSFKEVAKGIK
                     IKAILGVGDLETLRNVAKASLVCMMAGADFIKTSTGKENVNANLNNSLVMLRMIREFY
                     EYSGKKIGFKPAGGISTAKGVIEFLILVMEELGHEWFNPRLLRIGASSLLIDIERQLY
                     HHALGRYATKDRLAMA"
     gene            880..>2378
                     /locus_tag="EYO86_04525"
     CDS             880..>2378
                     /locus_tag="EYO86_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC42330.1"
                     /translation="MNNIIKNFKNIKYGPAPEDDKDVLKWIKNLSSPNRIYINGKWTL
                     SKSSKILQSINPSTNSKLFKLVVSSKKDVDSAVIAANKAYPKWSKLSSYKRSQYLYAL
                     ARLIQKHARFLSVLESIDNGKPIRETRDIDIPLVARHFYYHAGWAAKLKDSVKSLGVV
                     GQIIPWNFPLLMLSWKIAPAIACGNTVVLKPAEYTSLTAIFFAELCMKAKLPNGVINI
                     VTGDGSTGQHITNHPLVKKIAFTGSTEVGKKIIASTSNTNKKLTMELGGKSPFIVFED
                     ADLDSAVEGVVDAIWFNQGQVCCAGSRLLIQESIEKKFIKKLKQRMEKLRVGNPLDKS
                     IDIGAIVAPVQLKKIDSLVKKGVKEGAKLWQPSWSCPKEGLFYPPSLFTNVTPASYIA
                     QIEIFGPVLTSLTFRTPSEAVAIANNTPYGLAASIWSENINLALDVAPKVKAGVIWIN
                     STNLFDAACGFGGFKESGFGREGGSEGIRAYTKINLPVVRGSKKTNNKI"
BASE COUNT          812 a          370 c          472 g          724 t
ORIGIN      
        1 gctgttgaac ggcgcactat ttctttagtt aaaaggcgtt cgataaaaaa agactatcag
       61 gcggcttggt tattaaaggc aataacacta atcgacttaa ctacacttag tggagatgat
      121 acttttggga aagttgatcg tctttgtcaa aaagcgctta atccactagc tcacaatatc
      181 cttaatgtat tggatatacc acccaattca ttaacagttg gggctgtgtg tgtttatcat
      241 catttagttc atcaagccca aaagaattta gtaggaaaaa ttcctgtagc agctgtctca
      301 acaggttttc ctgcggggtt atcgtctttt tttacgcgaa aaaaagaaat ttcagattca
      361 attaaggccg gtgcaaagga aatcgacatt gtaatcaata gaggttttgt tttacaaaac
      421 aattggcaga aattgtataa tgaagttaaa agtttcaagg aagtagctaa aggaatcaaa
      481 ataaaagcaa ttctaggtgt gggagattta gaaacattgc gaaatgtggc caaagcttcc
      541 ttagtttgta tgatggctgg cgcagatttc attaaaactt ctacgggaaa agaaaatgtg
      601 aatgcaaatt taaataacag cttggtaatg ctccgtatga taagggagtt ctacgaatat
      661 tccggcaaaa aaattggttt taagccagca ggaggtatct ctacagccaa aggagtcatt
      721 gaatttttga ttttagtcat ggaagagtta ggtcatgaat ggtttaatcc tagattatta
      781 cgaattggtg cttctagttt attaattgat attgagagac aattatatca tcatgcatta
      841 ggacgttatg caacaaaaga cagattggcc atggcataaa tgaataacat tattaaaaat
      901 tttaaaaaca ttaaatatgg acctgcccca gaagatgata aggatgtttt aaagtggatt
      961 aaaaatttat ctagtcccaa tagaatttat attaatggaa agtggacttt gtctaaaagt
     1021 tcaaaaatat tacaatccat taatccttct actaattcaa aactattcaa attagtagtc
     1081 agttcaaaaa aagacgtaga tagcgcagtt atagctgcaa ataaggcata ccccaaatgg
     1141 tcaaaactat catcttacaa acgctcgcaa tatttgtatg cactcgctag actcattcaa
     1201 aaacatgctc gttttttatc ggttttagag tctatagata atggtaaacc tattcgggaa
     1261 acacgtgata tagatattcc tttagtagca cgtcattttt actatcatgc tgggtgggcg
     1321 gcaaaattaa aagattcagt taagtctttg ggagttgtgg gacaaattat accttggaac
     1381 tttcctttat taatgctttc ctggaaaata gctcctgcca ttgcttgcgg aaatacagtt
     1441 gttcttaaac ctgcagaata cacctctttg acagcaatat tttttgctga attatgtatg
     1501 aaagcaaaat tacccaatgg tgtcatcaat attgttacgg gggatggatc tacaggacag
     1561 catattacaa accatccttt agttaagaaa atagctttta ctggatcaac agaagttgga
     1621 aaaaaaatta ttgcttccac ttccaacaca aataaaaaat taacaatgga attggggggc
     1681 aaatcccctt ttattgtttt tgaagacgct gatttagaca gtgctgttga gggtgtcgtt
     1741 gatgctattt ggtttaatca aggtcaagtt tgttgtgctg gatcaaggtt gttaattcag
     1801 gaaagtattg aaaaaaaatt tataaaaaaa ttaaaacaac gaatggaaaa attacgtgta
     1861 ggcaatccac tggataagtc tattgatatt ggagcgatag ttgcaccagt tcaactaaaa
     1921 aaaattgata gtcttgttaa aaaaggtgtc aaggaaggcg caaaactatg gcaaccgtca
     1981 tggtcctgcc caaaagaggg tttattttat cctccttctt tatttacaaa tgtgacgcct
     2041 gcatcttaca tagcccaaat agaaattttt ggtcctgttt taacaagctt aacatttcgt
     2101 accccaagtg aagctgttgc aattgctaat aatacacctt acggacttgc agccagcatt
     2161 tggtcagaaa atatcaattt agctttagat gtagcgccaa aggtcaaagc tggagttatc
     2221 tggattaact caacaaatct ttttgatgcc gcttgcggtt ttggtgggtt taaagaaagt
     2281 ggatttggaa gagagggggg gagcgaagga attagagctt atacaaagat taatctgcca
     2341 gtagtgaggg gatcaaaaaa aacaaacaat aaaataaa
//
LOCUS       DTVV01000384            2325 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2141932, whole genome shotgun sequence.
ACCESSION   DTVV01000384 DTVV01000000
VERSION     DTVV01000384.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2141932"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="EYO86_04530"
     CDS             complement(<1..429)
                     /locus_tag="EYO86_04530"
                     /inference="COORDINATES: protein motif:HMM:PF02811.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIC42331.1"
                     /translation="MQYPFIHLRSQSSYSLSEGALKIEKLVNLAKKNNMPAIALTDNN
                     NMFGILEFSIACINNSIQPIIGTSINYLDIQTKEHPSQLNFLVMNEEGYKNLLYLSSI
                     SHTSGNNPIGVYHKDLKKYTNGLICYIGGEYNPLLILKHQN"
     gene            complement(429..1109)
                     /locus_tag="EYO86_04535"
     CDS             complement(429..1109)
                     /locus_tag="EYO86_04535"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC42332.1"
                     /translation="MTKNIISIYNLSKEYTSNNNSVVILKDISFKLYQGDIVSIVGPS
                     GSGKSTFLNILGLLDSSFKGQYDFLDKNIKYTSNDQKNIIRNNSIGFVHQFFHLIPEL
                     NVLENVTLPNLIQCNKVADSVKKAKSILTNFDLQDKILSKPHNLSGGEQQRVAIARAL
                     INKPSIIIADEMTGNLDEKISDSIFNFFLEQVRINNQSLIYVTHNNHYAKQANMKYQI
                     LNQKLKQI"
     gene            complement(1102..>2325)
                     /locus_tag="EYO86_04540"
     CDS             complement(1102..>2325)
                     /locus_tag="EYO86_04540"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02687.19,HMM:PF12704.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIC42333.1"
                     /translation="LIKRFLFSKKTDGFISIFSWFSIIGITIGVAAIIIVMAVMNGFR
                     EELISRLLGINGHLNIYSNSGQITKEEVNIIKSDFSDNQLIPLIQTQALVISNEFSKG
                     VFLRGYDNEYLDDLHFLKKNIVEGKLFGNNINDIVIGYVLANKFGLSVGDEIKIAIPK
                     TDNTIFGNIPRFKTLTVSGILNLGMYEYDSSFVFSNISIARKLLVLEDQNFNLIEIFT
                     QSPNNIEIIQDKVNRRIIANNFRLYTSSWKENNSTLINALNVEKNVMFLILTLIILVA
                     SMNIISGLIIFVKEKNKDIGILKTIGLSNKSLIKIFLSIGLIIGLIGTIFGGLVGVIF
                     SLNIKSIQFFIENILHTDLFAKEIYYLSNLPSRVDNLEVFYVLIISIIICLIATTIPA
                     YRSMKVDPIKSLKND"
BASE COUNT          839 a          313 c          296 g          877 t
ORIGIN      
        1 attttgatgt tttaaaataa gtaatggatt atactcgcct ccaatataac aaattaagcc
       61 attagtatat ttttttaaat ctttatgata aacaccaata gggttatttc ctgatgtatg
      121 agaaatggat gaaagatata aaagattttt ataaccttct tcgttcatca ctaaaaaatt
      181 taattgtgat ggatgctctt tagtttgaat atctaaataa ttaattgagg ttccaataat
      241 tggttggatg ctattattga tacacgcaat agaaaactcc aatattccaa acatattatt
      301 gttatcagtt aaagctattg caggcatatt attttttttt gccagattaa ctaatttttc
      361 aatttttaaa gcaccttctg aaagtgaata agatgattgg gaacgtaaat gaataaatgg
      421 atattgcatt aaatttgctt taatttttga ttaagaattt gatatttcat attagcttgc
      481 ttagcataat ggttattgtg agtgacataa ataagggatt gattattaat tctaacttgt
      541 tctaaaaaaa aattaaatat tgagtctgat atcttttcat ccaaattacc tgtcatttca
      601 tcagcaataa taatgcttgg tttgtttatg agggctctcg ctatagcaac acgctgttgc
      661 tcgccacctg ataggttatg aggtttggaa aggattttat cctgtaagtc aaaatttgta
      721 agaattgatt tagctttttt tactgaatct gctactttgt tacattgtat taaattagga
      781 agagttacat tttctaaaac atttaattca ggtattaaat gaaaaaattg atggacaaaa
      841 ccaattgaat tatttcgaat aatgtttttt tggtcattgg atgtatattt tatattttta
      901 tctaaaaaat catattgccc tttaaatgat gaatccaaca atcccaaaat attcaaaaaa
      961 gttgactttc cagaaccaga gggacctact atcgaaacaa tatctccttg gtataattta
     1021 aaagatatat cttttaatat tacaacacta ttattatttg aagtatattc ctttgataaa
     1081 ttataaattg aaataatatt tttagtcatt tttcaatgat ttaattgggt caactttcat
     1141 agatctataa gctggaattg ttgttgctat taagcaaatg ataattgata taattaggac
     1201 ataaaaaact tccagattgt caaccctgga aggaagattg gataaatagt aaatttcttt
     1261 agcaaataaa tcagtatgca atatattttc aataaaaaac tgaatagatt tgatatttaa
     1321 tgaaaaaatt accccaacta atcctccaaa tattgttccg attaatccaa ttatcaatcc
     1381 tattgataaa aaaatcttaa tcagactttt gttgcttaag ccaattgttt tcaaaatccc
     1441 tatgtcttta tttttttctt ttacaaaaat tattaaacct gaaattatat tcatagaagc
     1501 tactaaaata atcaaagtaa gtataagaaa cattacattc ttttctacat ttaatgcatt
     1561 aatcaatgtc gagttatttt ctttccatga tgaagtatat aatctaaaat tattagcgat
     1621 aattcttcta ttaactttat cctggataat ttcaatatta ttgggtgatt gtgtgaatat
     1681 ttctattagg ttaaagtttt gatcttcaag tactagtaat ttcctcgcaa tacttatatt
     1741 tgaaaataca aaacttgaat catattcgta catgcctaaa ttaagaatac ctgatactgt
     1801 taaagtttta aaacgaggaa tatttccaaa aatagtatta tcagttttag ggatagcaat
     1861 tttaatttca tcaccaactg ataatccgaa cttgtttgct aatacatagc caataacaat
     1921 atcgtttata ttgtttccaa ataatttacc ttctacaatg ttctttttaa gaaaatgtaa
     1981 gtcatctaaa tattcattat cataacctct taaaaataca ccttttgaga attcgttgct
     2041 tattactaaa gcctgagttt gaattaaggg aattaattga ttgtcagaaa aatcagattt
     2101 aatgatattg acttcttctt tagttatttg tcctgaatta ctgtaaatat ttaaatgtcc
     2161 attaataccc aacaatcttg aaattaattc ttctctaaaa ccgttcatta cagccatcac
     2221 tataataatt gcagcaacac cgattgtaat gcctatgatt gaaaaccaag aaaaaataga
     2281 gataaaccca tccgtttttt tagaaaaaag aaaacgttta attaa
//
LOCUS       DTVV01000385            3175 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2143253, whole genome shotgun sequence.
ACCESSION   DTVV01000385 DTVV01000000
VERSION     DTVV01000385.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2143253"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="EYO86_04545"
     CDS             complement(<1..640)
                     /locus_tag="EYO86_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HIC42334.1"
                     /translation="MFKAVEKDIIFNANKSFTEIVDKDYDYFCERISRLNINPESLKE
                     KVSSFNVALPSWGVGTGGTRFARFPGIGEPQNIFEKIEDCAVINDLVGITSSVSPHFP
                     WDDVDDYPALRQFTESYGINFDIVNSNTFQDPAGSKGSFKYGSLTHIDQSVREQAIKM
                     NIDCIEKGITLGSTGLTVWIADGGNFPGQQNFSKSLERYVDSMQSIYKKLPED"
     gene            complement(633..1028)
                     /locus_tag="EYO86_04550"
     CDS             complement(633..1028)
                     /locus_tag="EYO86_04550"
                     /inference="COORDINATES: protein motif:HMM:PF07100.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42335.1"
                     /translation="MNEIGKNIWVFPDAYLPAKGQPYKTSRTGDQFSHESLCMINASD
                     KDAFVKISFYYEDQDPYENYEHKVEAKRSLHLRLDKLVLPNYGNLKLEIPYSIIVKSN
                     IKIVAQLSRLDTTSNYNSFMTSLGWGTNV"
     gene            complement(1021..2004)
                     /locus_tag="EYO86_04555"
     CDS             complement(1021..2004)
                     /locus_tag="EYO86_04555"
                     /inference="COORDINATES: protein motif:HMM:PF02653.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC42336.1"
                     /translation="MNNKILLNPPKNILNIFYKKEFILFLVLILLIIFNSQISPYFLD
                     LYNLIDSTFLFSEKAIIALSMTFVIIARQIDLSVASIIAVSSCVLGYTTSIGLPTFYV
                     ISLTLFSGGVCGAFNGFIITRYAIPSIIVTIGTMSLFRGLAYSFLEDQYYNKFPDIVV
                     KLGSDYLFGYIPYSFLTFIILTLCFAFVLHKTIIGKRIFAIGNNPDAAKYTGIDVDKY
                     TFVLFTMNGIFSALASIFLSGRLSSVRPNIAFGWELQIITMVVFGGVYIYGGSGSILG
                     VFISIIILGMLSFGLGLLNVPGVGIIIFTGLLLIISIGLPSVIKYFRNQNE"
     gene            complement(2006..2980)
                     /locus_tag="EYO86_04560"
     CDS             complement(2006..2980)
                     /locus_tag="EYO86_04560"
                     /inference="COORDINATES: protein motif:HMM:PF02653.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC42337.1"
                     /translation="MILKFIKNRDFILFTIIFIILVAVGISNPNFVTLKSLSDVLTDT
                     SILIILALSQMIVILIRAIDLSVASNLALSGMLISLLSQIYPDLPLIFFILLSCGIGT
                     LLGLFNGLVIAVLKVPFIVTTLGTLSIYRGLIYFVSDGKQVYSHEFSDSFLSFIHVRF
                     LSLTSLFWFAVCVFIIIYILLNYTKFGRNIYALGGNPEAAKYIGINENRYIIAAYVLS
                     GFFAGLCGYLWVARYSVASTQIAIGFELTVISACVVGGISVMGGLGTVTGCLLGALLI
                     GLIRNALPAIDISQFWQLAISGAIILIAIIINTKTEQRKEKSIMLEKD"
BASE COUNT         1206 a          570 c          448 g          951 t
ORIGIN      
        1 aatcttctgg taatttttta taaattgatt gcatagaatc aacatatctc tcaagagatt
       61 tagaaaaatt ttgttgacca ggaaaattac cgccatctgc aatccacaca gttaatcctg
      121 ttgatccaag cgttataccc ttttcaatac aatcaatatt cattttgata gcctgttctc
      181 ttactgattg atcaatatgc gttaagcttc cgtatttaaa agatccttta cttcccgctg
      241 ggtcttgaaa cgtattggaa ttaacaatat caaaattaat tccatatgac tctgtaaatt
      301 gtcttagagc aggatagtca tctacatcat cccaaggaaa atgaggggag acacttgagg
      361 taatacccac aagatcatta atcactgcac aatcttcaat tttttcaaaa atattttgtg
      421 gctctcctat ccctggaaaa cgcgcaaacc gcgttccgcc tgttcccaca ccccatgagg
      481 gtaatgctac attaaaacta gaaacttttt ctttcaggct ttcagggtta atatttaaac
      541 gagatattcg ctcgcaaaaa taatcataat ctttatctac aatttcagtg aatgatttat
      601 tcgcattaaa tattatatct ttttctacag ctttaaacat tggttcccca tcctaacgaa
      661 gtcataaaag agttataatt agaagttgta tctaatcgtg aaagttgtgc aacaatttta
      721 atattgcttt ttactataat agaatatggt atttcgagtt tcaagttccc ataattaggt
      781 aaaaccaatt tatctaatct taggtgcaaa cttcttttgg cttcaacttt gtgctcataa
      841 ttttcatacg gatcctgatc ttcatagtaa aaagatattt ttacaaacgc atctttatca
      901 gaagcattaa tcatacaaag agattcatgg ctaaattgat cccctgtacg agaagtttta
      961 tacggttgtc ctttagctgg taaataagca tcaggaaaaa cccaaatatt ttttcctatt
     1021 tcattcattt tggttacgaa aatatttaat aacagatgga agaccaatac taataataag
     1081 taacagtccg gtaaagataa ttataccaac accagggaca tttaataaac ctagtccaaa
     1141 agataacatt cccaaaataa taatagaaat aaaaacacca agaatagaac cggagccacc
     1201 ataaatatac acaccgccaa aaactaccat cgttataatc tgaagttccc aaccaaatgc
     1261 aatatttggt ctgacactag ataatctgcc agaaagaaaa atagaagcaa gcgcagagaa
     1321 aataccgttc atcgtaaata aaacaaaagt atatttatca acgtcgatac ctgtatattt
     1381 tgcagcatcg ggattatttc caattgcaaa aattcttttg ccaataatag ttttatgtaa
     1441 aacaaaagca aaacataaag tgagaataat aaaggttaaa aaagaataag gaatgtatcc
     1501 aaaaagataa tcactaccta atttaacaac aatatcagga aacttattat aatattgatc
     1561 ttctaaaaag gaatatgcca gtcctcgaaa taaagacatg gtgccaatgg tgacaataat
     1621 cgaaggaata gcataacgtg taataataaa accattaaat gctccgcaca ctcctccgct
     1681 gaacagagtt aatgatatga cataaaaagt aggaagccct atacttgttg tgtagcctaa
     1741 aacgcatgat gaaacagcaa taattgatgc aacggataaa tcaatttgcc gggcaataat
     1801 gacaaatgtc atacttaaag caataatagc cttttcactg aataaaaaag tactatcgat
     1861 tagattgtat aagtctaaaa aatatgggga gatttgagag ttaaagataa ttaataatat
     1921 taaaactaaa aataagataa attccttttt atagaaaata tttaatatgt ttttaggggg
     1981 gttaagtaga attttattat tcatactaat ctttttctaa cattatactt ttttctttac
     2041 gctgttctgt tttcgtatta ataataattg caatcaaaat aattgctcca gatatcgcta
     2101 actgccaaaa ctgtgaaata tcaatagcgg gcagtgcatt tctaattaat cctattaata
     2161 acgcacccag taaacatcca gtaacggttc caagaccacc catcacactg attcccccta
     2221 ctacgcatgc tgatatcaca gtaagttcaa agccaatagc aatctgtgtt gaggcaactg
     2281 aataccgtgc cacccataaa taaccacaca aaccagcaaa gaagcctgat agaacataag
     2341 cagctataat ataacggttt tcattaattc ctatatactt agcagcttca ggatttcctc
     2401 ccaaagcata tatattgcga ccgaattttg tataatttaa aagtatgtat ataattataa
     2461 aaacacagac agcaaaccaa aacaagcttg tcaaagataa aaatctaaca tgaataaaag
     2521 ataaaaagct atctgagaat tcatgggagt acacttgttt accatcacta acaaaataga
     2581 ttaatcctct gtaaatactt agtgttccta gagtcgtaac tataaaagga acttttaaaa
     2641 cagcaattac aagaccatta aataatccca gcaatgttcc tattccgcag gacaaaagaa
     2701 taaaaaatat aagaggaaga tcaggatata tttgcgagag taatgatatt aacatgcccg
     2761 atagcgctaa attggacgcc acagataaat caatagctct aattaatatg acaatcattt
     2821 ggcttagtgc caaaataatt aaaatggatg tatcagttaa aacatccgat aaactcttaa
     2881 gagtaacaaa gttaggattg gatatcccaa cagcgacaag aataataaag ataattgtaa
     2941 ataaaataaa atctctattt ttaataaatt ttaaaatcat tatttattca ccactcgcat
     3001 aggaaagaat ttcttctgaa gaagtttctg aaacatctaa tatttttcta atcaaacctt
     3061 tatagacaac tgcaacacgg tcgcacattc ccataatttc cggtaattca gctgaaatca
     3121 taataatgga cattccttca tttaccaatt ctcccataaa ttcgtgaaca gcgga
//
LOCUS       DTVV01000386            1012 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2145329, whole genome shotgun sequence.
ACCESSION   DTVV01000386 DTVV01000000
VERSION     DTVV01000386.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2145329"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(63..413)
                     /locus_tag="EYO86_04565"
     CDS             complement(63..413)
                     /locus_tag="EYO86_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42338.1"
                     /translation="MVETLFLIIIGAGIGLYQDMNKPTQFELNMEDGTNTEVILQKNS
                     SYSCPVYCEADHIHIAVSCEDNCKTDHKNYHLHNVTEIDEGFATFCSKKIIAMNKIST
                     KKKLPDILSTSKSE"
BASE COUNT          373 a          145 c          139 g          355 t
ORIGIN      
        1 aaagcataat aaaaaaagcc cggcagtacc gggctttttt attataagtg gaatttaggt
       61 tactactcag atttggatgt actcaaaata tcaggaagtt ttttcttcgt agatatttta
      121 ttcatagcaa taattttttt tgagcaaaag gtcgcaaacc cctcatcaat ttcagtaaca
      181 ttatgtaaat ggtagttttt atgatcagtt ttacaattat cttcgcatga taccgcaata
      241 tgaatatgat ctgcttcaca ataaaccgga cacgaatagc tactattttt ctgcaaaata
      301 acttctgtat tcgtaccatc ttccatattc agttcaaatt gcgtaggttt attcatatct
      361 tgatataatc caattcctgc accaataata attaaaaata aagtttctac catttttcca
      421 tctcatttaa taaattgtta atgtttcctc ctaactccta gataaatata tgagtcaata
      481 agaaataata tttacgcctg cgtcacataa ttacatcgga aaaattaatt gtgatagaga
      541 tcacaggtat taatatttca aattctgaaa agctgttcaa taattaactt tattactata
      601 aatatatcaa acaaattata gaattggtta aatatatttc atatgataaa ctataatatt
      661 ttatcctata tccttataat acaactagtt ataatggatt gttcacaaat atatttaaag
      721 taatacttta aatgcttgca tttattaatt gaaagcttgt aatattgttt acaatcaaag
      781 cctcacaagg tagataactt gtgaaatgta aggttgtacg taaatttggg ctaaaaagat
      841 tatagatgat agctttaaat catgtataaa aaaacaaaaa caaaaaagga gaaaaacaat
      901 atgaagaatg ttaagacact cttgatatta ctatcaatta gtacatttat atttgctagt
      961 acaacaagaa ctgatgcctt gggcggtgct ggattttggg cggatgatta tg
//
LOCUS       DTVV01000387            1308 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2146876, whole genome shotgun sequence.
ACCESSION   DTVV01000387 DTVV01000000
VERSION     DTVV01000387.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2146876"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="EYO86_04570"
     CDS             complement(<1..1034)
                     /locus_tag="EYO86_04570"
                     /inference="COORDINATES: protein motif:HMM:PF00162.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIC42339.1"
                     /translation="MVKIKKIHDFNLHSKKVLVRLDLNVPLNNNKILDNTRIKASLAT
                     LKEIIKKKGTPVIISHFGRPNGKVNKRFSLKKILKSLSKHLGKKVIFCNQCIGLKVKK
                     VLKDIKPGQIVLLENLRFHKEETENSSKFAKDLSKYCDIYINDAFSVSHRKHSSIDKI
                     TRFIPSGMGKNMENEINNLNKYLKKPKKPITAIIGGSKMDDKINLINTLVNKCRYLIL
                     GGGVANTFLKSKNFNIGKSIYEKKQIKNAIKIQNKAKKNNCKIILPLDVVVSEKKKNL
                     DISSINKNHRILDLGKNTLNMIYDIISNSKTVIWSGPVGYYEKKPYDRGTNKLANFIN
                     SKKTLISIAGG"
BASE COUNT          420 a          170 c          141 g          577 t
ORIGIN      
        1 ccaccagcaa tacttattaa tgtttttttt gaattaataa aattagctaa tttatttgtt
       61 cctctatcat aaggtttttt ttcataataa cctacagggc cactccaaat tacagtttta
      121 gaattactaa ttatatcgta aatcatatta agagtatttt tacctagatc gagaattcgg
      181 tgatttttat ttatgctaga aatatctaaa tttttttttt tttcagaaac cacaacatct
      241 aatggcagta ttattttaca attatttttt tttgccttat tttgaatttt aattgcattt
      301 tttatctgtt ttttttcata aatagattta ccgatattaa aattttttga tttcaaaaaa
      361 gtatttgcta caccaccacc taaaattaaa tatctgcatt tattaacaag tgtattaatt
      421 aggtttattt tatcatccat ttttgaccca ccaattatgg cagttattgg tttttttggt
      481 ttttttaaat atttatttaa attattaatt tcattttcca tattttttcc cattccagat
      541 ggtataaacc ttgtaatttt gtcaattgaa gaatgttttc tatgactaac actaaatgcg
      601 tcatttatat agatatcaca atatttactt agatcttttg caaatttact actattttca
      661 gtttcctctt tgtggaatct tagattttct agtaaaacaa tttgaccagg tttaatatct
      721 tttaaaactt tttttacttt taaacctata cattgattac aaaaaataac ttttttacca
      781 agatgcttcg ataaactttt taatattttt tttaaagaaa atcttttatt tactttacca
      841 tttggtcttc caaaatgact gatgataact ggtgtgcctt ttttttttat gatttccttt
      901 aaagttgcca aagaagcttt aattcttgta ttgtctaaaa ttttattatt gttaagaggt
      961 acgtttagat ctaatctgac taaaactttt tttgaatgta aattaaaatc atgaattttt
     1021 tttattttta ccattatgta tttatatatt gtagaatttt ttttattaat ttatcactat
     1081 gaaaaataaa ttttattttt ttttatttct tttatttttt aactataact taatatttgc
     1141 taatcaaatt gtgattgaag aagatgaatg gccatgcaag ctacatcatt tggagccacc
     1201 tcaacaagca gatttttggc cagggaaaaa tattgattta gactctaaat ggaaaaaaga
     1261 cgacgatata agagatttgg taaactatat tacgaatcat gcaaactc
//
LOCUS       DTVV01000388            1535 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2147471, whole genome shotgun sequence.
ACCESSION   DTVV01000388 DTVV01000000
VERSION     DTVV01000388.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2147471"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="EYO86_04575"
     CDS             complement(<1..1104)
                     /locus_tag="EYO86_04575"
                     /inference="COORDINATES: protein motif:HMM:PF06213.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42340.1"
                     /translation="MLENDEIVDQFKKSLSATIKSIGKSNTIEVNFVQDGPSIDGDII
                     NLNEPNLQSLKKNLNYMRAEADSMALEIRLHTKEIHQEYLGNNDVVNEIFNAVEQSRI
                     EAQGSHIFKGIKLNILNKHKFDIQNTEDKNNKDKEDYKEIIEAFRYVSYSELTGEILG
                     GKFSAYKKLIQKKLGHEYNDFFKKLRNNITNQKNFADLLQTFLDNLGYYDHNNKQDGI
                     DNEDSSEDKDLHDEDNKSKNQDSKTDTKHDDEASSSDLQQASSMDEDQKMGDEANEND
                     LEYFPKIESLDKLENYKFYTDKFDEVVKAEELCDLKELDRLRLSLDQQVFTFKPLIAK
                     IANQLQRKLLAQQTRQWEFNLEEGYLDTSRLARI"
     gene            complement(1091..>1535)
                     /locus_tag="EYO86_04580"
     CDS             complement(1091..>1535)
                     /locus_tag="EYO86_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HIC42341.1"
                     /translation="FATANTVGLGDTSGLYHGTQQINQGQMDRWNIVTTLNYLNNIEE
                     EEIVLSKNKKLNNKEGKEIIRCMVSTADLTRSGFINGDISTVMSPRTVITWAENYLLF
                     NDIEYSFKLAFLNRCDELERSIIEEYYQRSFGIDLSDAKVVNVGE"
BASE COUNT          452 a          266 c          207 g          610 t
ORIGIN      
        1 gatccttgct aagcgtgaag tatcaagata gccttcttca agattgaatt cccattgtct
       61 ggtttgttga gctagcagtt ttctttgtaa ttgattggcg attttggcaa taaggggctt
      121 aaaagtaaat acttgttgat ccagagataa tcgtaatcga tctaattctt ttaagtcaca
      181 gagttcttct gcctttacta cttcatcaaa tttatcagta taaaatttat agttttcaag
      241 tttatctaga gattcaattt ttggaaagta ttcaagatca ttttcatttg cttcatctcc
      301 cattttttga tcttcatcca ttgaacttgc ttgttggagg tctgaagaag aggcttcatc
      361 atcatgtttt gtatccgttt tactatcttg atttttcgat ttattgtcct catcatgtag
      421 gtccttatcc tcactgctat cttcattatc aataccatct tgtttattgt tatgatcata
      481 ataacccaaa ttatctaaaa aagtttgcag tagatcagca aaattttttt gattggttat
      541 attgtttcta agttttttga aaaaatcatt atattcatga cctaattttt tttggattag
      601 ttttttatat gcactaaatt taccacctag tatttcacca gttaactcac tgtagctcac
      661 atagcgaaaa gcttcaataa tttccttgta gtcttcttta tccttattat ttttatcttc
      721 ggtattttga atatcgaatt tatgcttatt taatatattt aatttaattc ctttgaagat
      781 atgcgatcct tgagcctcaa tgcgtgattg ctcaacagca ttaaatattt catttacaac
      841 atcattgttg cctagatact cttgatgaat ttcttttgta tgaagtcgaa tttctaatgc
      901 cattgagtct gcttcagccc gcatataatt taaatttttc ttaagagatt gaagatttgg
      961 ctcatttaaa ttaataatat ctccatctat agaagggcca tcttgaacaa agttaacttc
     1021 tatagtgtta gacttaccta tggatttaat tgttgcagag agtgatttct taaattggtc
     1081 gactatttcg tcattctcca acattaacta ccttggcatc cgataaatca attccaaatg
     1141 aacgttgata atactcttca attattgatc tttccaattc atcacatctg tttaagaatg
     1201 ctaatttaaa tgaatattca atatcattga ataacaaata attctcagcc catgttatta
     1261 cagttcttgg actcattaca gtagaaatat caccattaat aaaaccagaa cgagtcaaat
     1321 cagccgtact aaccatacag cgaataattt ccttaccttc tttattatta agttttttat
     1381 ttttacttaa aacaatctcc tcttcttcta tgttgtttaa ataatttaag gttgtaacaa
     1441 tattccatct atccatttga ccttgattta tttgttgcgt gccgtgataa agacctgaag
     1501 tatcacctag tccaacagta ttagctgtag caaac
//
LOCUS       DTVV01000389            2185 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2150772, whole genome shotgun sequence.
ACCESSION   DTVV01000389 DTVV01000000
VERSION     DTVV01000389.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2150772"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1614..2066
                     /locus_tag="EYO86_04585"
     CDS             1614..2066
                     /locus_tag="EYO86_04585"
                     /inference="COORDINATES: protein motif:HMM:PF05099.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIC42342.1"
                     /translation="MLNSLKFFFHQSGEIHQNNTDDLHLLSGLMLETANIDGHVDQLE
                     VDKISKVLIDIFHEEPSAVEAELKKCLEELEEHKSFHSFTSQINQSFSNEKKIILLEI
                     LWEIILEDGELHDFESNLMRRLAGLLYISDVQCGNAKKRALKKSQLTK"
     gene            2076..>2185
                     /locus_tag="EYO86_04590"
     CDS             2076..>2185
                     /locus_tag="EYO86_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIC42343.1"
                     /translation="MTYVVDEKCIKCKLMDCVEVCPVDCFYEGENMLVIHP"
BASE COUNT          811 a          281 c          349 g          744 t
ORIGIN      
        1 attttcacat catttcagaa gatacaaata ttattcctta tttaaataat aaggcgacta
       61 taagtttgtt atgggatatt tgtcgtattc cagattttca aaaattttcc actgattctt
      121 atattgattt tttaaaaaat atttttttaa cattagtgga aaatgatggg attttacctg
      181 agtcttggtt ggaaaataga gtttttcgat tagaaaatta caagggtgga attgaagagc
      241 tatcaattaa aatatccact ataagaactt ggacatacat tgctaatcaa tctaaatgga
      301 taaaaaatta tcagttctgg caagaaaaaa cccatgatat tgaaaataat ctttcagact
      361 gtttacatac cagcctaaca aatagatttg ttgatttttc agcaagttat tttattgata
      421 cgaaaatgcg aggtgaaaat cctgttatta cagttgatga aaataaatct ataaaattaa
      481 atggacaaaa ttatggatat attaatggtt ttgatctaga attaaatata tcaaacttaa
      541 aatcattatt ctctctaacc aatgttaaaa aatccattcg ttccatgatt gaagaaaaaa
      601 ttgatatttt tttacaagct ccttcggatt caattaattt gggggatatt cgttcattca
      661 aaattaatga gccagtaaat atttattggg gtgacgaacc tgttggaaaa ttagtaaaag
      721 gaattaatat tttttcacct aaagcagaag cacttaatac agagttttta gaatcagata
      781 aaaaaatatt aattacaaga aaattacaac aatgggtaga gaaaaaaatt ttcactactt
      841 taaaacctat tacagaagat ttagaccaaa ctataagttc agacgtaagg gccattgttt
      901 ttaatgtttt tagcgctctt ggtacaatgt tgattggtga ccatgacaat actataaaaa
      961 atatgacaga gcacgataag gcaaccattt ctcgtaccgg tcttcgtata ggggtaaaat
     1021 ttttcttcat gcctaacttt ttaaaaaaac aaccaatgga actaaatgct cttttatgga
     1081 aaatttttta tgaatgggat attgattatg cttacccact gcctacagat ggaagagtct
     1141 cttttataac tgaaacaaaa atgcctaatt cttattggtc ggctattgga tactattgct
     1201 tggacaattt tgcaattcgt gttgatgttt ttgaaagggt tttttttctt gcaagacaaa
     1261 aaatcaaata cggacctttt attgaatctt ccgacatgat gaatcccatt ggatgtaatt
     1321 ctgaccaatt aaagaatatt atgcaatttt gtggattaga aagtttgaat cttggtgaca
     1381 acaaaaaatt atttttcttt aagcttaaaa aacaaaataa atctattaaa aaaatggctg
     1441 ataataagaa gggtattata aaaataaaaa ataaaaaaat agccaaaatt acaaaaacaa
     1501 agattaaaaa attaacttcc aagaaagaaa ttaaggctga tcctaattct ccttttgcag
     1561 ttttgggaaa actactttaa taaatatata taatctttat aatcggataa tcattgctta
     1621 actctttgaa attttttttt caccagtctg gagaaataca ccaaaataat acagatgatc
     1681 tgcacctttt atctggttta atgttagaaa cagcaaatat tgacggtcat gttgatcagc
     1741 tggaagttga taaaatatct aaagtgctaa ttgatatatt tcatgaggaa ccttctgctg
     1801 tagaagcaga attaaaaaaa tgtttggaag agcttgaaga acacaagtct tttcactctt
     1861 ttacatcaca gatcaaccaa tctttttcta atgaaaagaa aattatttta ctagaaatat
     1921 tgtgggaaat tattttggaa gatggggaac tgcatgattt tgagtctaac cttatgaggc
     1981 gtttggcagg tttgttgtat atatcagatg ttcagtgcgg taatgccaaa aagagagctc
     2041 taaaaaaatc acaattaact aaataagtta agagtatgac gtatgttgta gatgaaaaat
     2101 gtattaaatg taaacttatg gactgtgttg aagtttgccc tgttgattgt ttttatgaag
     2161 gtgaaaatat gctagttatt catcc
//
LOCUS       DTVV01000390            2873 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2152903, whole genome shotgun sequence.
ACCESSION   DTVV01000390 DTVV01000000
VERSION     DTVV01000390.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2152903"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="EYO86_04595"
     CDS             complement(<1..678)
                     /locus_tag="EYO86_04595"
                     /inference="COORDINATES: protein motif:HMM:PF13491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIC42344.1"
                     /translation="MNINNIANKTLDFTVKRIAELTGILLVVLSILLFISLVGYYPKD
                     PNFIFSDPTEKIRNILGFRGSFVSDLFFQSIGLISILVSITIFFTGINLFRIKKFLLI
                     LENLFYTVLYSILGSLFFSTFYPDAFWLINNGNGGFIGKFLQESFLSSLININKNVFY
                     YLLIFLILFIFLISINFSIKYLFNLLKKLFGFIKNLNNKTDDFGGEAVDNYKTEIKPS
                     EILVQEDL"
     gene            745..1836
                     /locus_tag="EYO86_04600"
     CDS             745..1836
                     /locus_tag="EYO86_04600"
                     /inference="COORDINATES: protein motif:HMM:PF01494.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HIC42345.1"
                     /translation="MNICLIGDGLTNLVLAKILANKNISISVCFESKKKKKSTSRTIG
                     ISKNNFDFFKSNIIDIKKISWPINYIQIFNELNQIEEILNFKSTDSQLFFIVKHSELY
                     ELVQKDIKKNKFIKKIKIKKSFYDEIIENNKFDLIINSETQNKISKKIFFKRITKDYK
                     SVAFTTIVKHQKCINNKAVQIFTSCGPLAFLPYSNTETSIVFSVLDEKKNKSEKEIKE
                     LIIKYNKYYKINSFKNFEKYNLKFSILRKYYHKNILCFGDNLHNIHPLAGQGFNMTLR
                     DIKILSDLIDEKIDLGLPLDSSILKEFESKTKHLNYIFSSGINFIHEFFKFDNKYGNN
                     YSKEILKYLGKNNLFNKYISKFADQGLVL"
     gene            complement(1839..2471)
                     /locus_tag="EYO86_04605"
     CDS             complement(1839..2471)
                     /locus_tag="EYO86_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HIC42346.1"
                     /translation="MKQLPKTISIFPLSNFIFFPQTTVPLNIFEPRYIQMVDDAMKTD
                     RIIGMVQPKKTNQTIPILYNIGCAGKITSFNETEDGGYLIVLDGISRFKILEELNNDK
                     LYRECKVNFDKFSNDINNQKEEIKFSDLELIFKNLKSLFNKQGYTINWKELEKRSLDQ
                     TINTLSMASPFSLEEKQVLLETINLNERKQKLEEILKTYTLDNFSNKTLQ"
     gene            2515..>2873
                     /locus_tag="EYO86_04610"
     CDS             2515..>2873
                     /locus_tag="EYO86_04610"
                     /inference="COORDINATES: protein motif:HMM:PF13181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC42347.1"
                     /translation="MDKNLKLLESEIKNLSFLLKIGNHNRLIKRTSKLISKYPQITAF
                     RNFLGLSLEMQGKLYHAEKVYLKALERDPNNINILVNLGGVYRKINNLSKSEELLKNA
                     LNIDPNDLFGLRNYGEL"
BASE COUNT         1162 a          305 c          322 g         1084 t
ORIGIN      
        1 caaatcctct tgaactaata tttcactagg tttgatttct gttttatagt tgtctacagc
       61 ttctccacca aagtcatctg ttttattatt taaattttta ataaaaccga aaagtttttt
      121 aagtaaatta aataaatatt taatactaaa attaatgctt attaagaaga taaataaaat
      181 taagaaaatc agcaggtaat aaaaaacatt tttgtttata ttgattaagg atgataaaaa
      241 actttcttgt aaaaattttc ctataaatcc gccattacca ttatttataa gccagaaagc
      301 atctggataa aatgtagaaa aaaacaacga acctaaaata gagtacaaaa cagtgtaaaa
      361 taaattttct aaaattaata aaaatttttt tattctaaaa agattaattc cagtaaaaaa
      421 aatagttatt gatactaaaa ttgaaatcaa tccaatggat tgaaaaaaca aatctgaaac
      481 aaagctaccc ctaaatccta aaatatttct tatcttttca gtagggtcag aaaaaataaa
      541 atttggatct ttcgggtaat aaccaactaa tgaaataaat agcaatattg ataaaacaac
      601 taataatata cctgtaagtt cagcaatcct ttttacagta aaatcaaggg ttttatttgc
      661 tatattattt atattcattt tattatgaat ctattttgta actttgtatc atttaataat
      721 tattgttaaa attaaatttt aattatgaat atttgtttaa ttggcgatgg attaactaat
      781 ttagttttag caaaaatttt agctaataaa aatattagca tatctgtgtg ttttgagtca
      841 aaaaaaaaaa agaaatctac ttcaagaact attggaattt caaagaataa ttttgatttt
      901 tttaaatcta atattataga tattaaaaaa attagctggc caattaatta tattcaaatc
      961 tttaatgaat taaatcaaat tgaagaaata ttaaatttta aatcaactga tagtcaattg
     1021 ttttttatag ttaaacatag tgagctatat gaattagttc aaaaggatat aaaaaaaaat
     1081 aaatttataa aaaaaattaa aataaaaaaa tcattttatg atgaaataat agaaaataat
     1141 aaattcgatt taattattaa ctcagaaaca caaaataaaa tttcaaaaaa aatttttttt
     1201 aaaagaatta ctaaggatta taaaagtgtt gcatttacta caattgttaa gcatcaaaaa
     1261 tgtataaata ataaagctgt tcaaattttt actagttgtg gtccacttgc ttttttacct
     1321 tattctaata ctgaaacttc aatagttttt tccgttttgg atgaaaaaaa aaataaatca
     1381 gaaaaagaaa taaaagaact tattattaaa tataataaat attacaaaat taattctttt
     1441 aaaaattttg aaaaatataa tttaaaattt tcaattctta gaaaatatta tcataaaaac
     1501 atattatgtt ttggtgataa tcttcacaat attcatcctc tggcaggtca aggatttaac
     1561 atgactttga gagatataaa aatattgtca gatttaattg atgaaaaaat agatcttggt
     1621 ttacctttag attcatcaat attgaaagaa tttgaatcaa aaacaaaaca tctaaattat
     1681 attttttctt caggaattaa ttttattcat gagtttttta agtttgataa taaatatggc
     1741 aataattact ctaaggaaat attaaagtat ctaggaaaaa ataatctttt taataaatat
     1801 atttctaaat ttgctgatca gggtcttgtt ttataaattt actgaagtgt tttgttgcta
     1861 aaattatcga gagtataagt ttttaatatt tcttctaatt tttgttttct ttcatttaaa
     1921 ttaatagttt ccaacaaaac ctgtttttct tctaatgaaa aaggagaagc cattgaaaga
     1981 gtatttattg tttgatctaa gcttcgtttt tcaagttctt tccaattaat tgtatagcct
     2041 tgtttgttaa ataaagattt taaattttta aatattagct ctaaatcaga aaacttaatt
     2101 tcttcttttt gattatttat atcatttgag aatttatcaa aatttacttt gcattctcta
     2161 tagagcttat cattatttaa ctcttctaaa attttgaaac gactaattcc atctaaaaca
     2221 attaaatacc ctccatcttc tgtttcatta aaacttgtaa ttttgccagc acatccaata
     2281 ttatataaaa ttggaatagt ttgattagtt ttttttggtt gtaccattcc tattatccta
     2341 tctgttttca ttgcatcatc aaccatctga atgtatcttg gttcaaaaat atttagagga
     2401 acagttgttt gaggaaaaaa aataaaattt gataacggaa aaattgaaat agtttttggt
     2461 agctgtttca tataatattt atatttaaca atattatatt ctaatttctt taaaatggat
     2521 aaaaatttaa aactactaga gtcagaaata aaaaatttaa gttttttact aaaaattgga
     2581 aatcataata ggctgattaa aagaactagc aaattaattt caaaatatcc ccaaattacg
     2641 gcatttagaa attttttagg tttgtcactt gaaatgcaag gtaaacttta tcatgccgag
     2701 aaggtttatt taaaagcact tgaaagagat ccaaataaca taaatatttt ggtcaattta
     2761 ggtggagtgt atagaaaaat aaacaatctt tcaaaatccg aggaattatt aaagaatgca
     2821 ttgaatattg atcctaatga tttgtttggt ttgagaaatt atggcgaatt aaa
//
LOCUS       DTVV01000391            1464 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2153859, whole genome shotgun sequence.
ACCESSION   DTVV01000391 DTVV01000000
VERSION     DTVV01000391.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2153859"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(78..779)
                     /locus_tag="EYO86_04615"
     CDS             complement(78..779)
                     /locus_tag="EYO86_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bb3-type cytochrome oxidase subunit IV"
                     /protein_id="HIC42348.1"
                     /translation="MEQSTESVQVTIPPNKGMVSDWASDQKAFKGVPWGKAMMWIFLV
                     SDTFVFSIFLISYMTVRFSTKAEWPNPSEVFALHVGHVSVPLLLIAIMTFILITSSGT
                     MALAVKYGYEKNRKKCAILMFATAVFGASFVGMQAFEWTKLIMEGVRPWENPFGAPQF
                     GSIFFMVTGFHGTHVTIGVIFLIIMARKAWRGDFDTGSRGFFTSQKSNYEAIEIMGLY
                     WHFVDLVWVFIFSRT"
     gene            complement(793..1428)
                     /locus_tag="EYO86_04620"
     CDS             complement(793..1428)
                     /locus_tag="EYO86_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative cytochrome c oxidase polypeptide
                     CoxN"
                     /protein_id="HIC42349.1"
                     /translation="MKKKESIFQLLTQKPWDPSQAKLDNLHKGETINLSKGKLGLRFI
                     MIVCTIFFCLFIVTYADRMVYPDWQIMPEPLLLWFNTFILLVSSVAFVSVQIASKNKE
                     FEKVKSRMLLIGVLALIFLLGQLMVWQNLISSGYYLSTNPANAYFYLFTALHGLHLIG
                     GLFYWNIAVKKVWIPSEVAVVKTQHTVELCAIYWHFLLAVWVVLFGLMLFS"
BASE COUNT          506 a          285 c          224 g          449 t
ORIGIN      
        1 cgaaactttt aagattttat gaatgtaagt tttctttaat ttcgaagatt ttttcgccaa
       61 ataattaggc tatttttcta agttctcgaa aaaataaaga cccaaactaa atcaacaaag
      121 tgccaatata aacccatgat ttctattgct tcatagtttg atttttgtga tgtgaaaaaa
      181 cctctgcttc cagtatcaaa atctccgcgc catgcttttc ttgccattat aattaaaaag
      241 ataacgccta ttgtaacatg tgttccatga aagccagtga ccataaaaaa tattgatcca
      301 aattgaggtg ccccaaacgg attttcccaa ggacgtactc cttccatgat taattttgtc
      361 cattcaaatg cttgcatacc aacaaaggat gcaccaaaaa cagcggttgc aaacattaaa
      421 atagcacact ttttgcggtt tttttcgtat ccatacttta ctgcaagggc cattgttcca
      481 ctactggtga ttaatataaa agtcatgatt gcaattaaaa gtaaaggcac actgacatga
      541 cctacatgta aagcaaatac ttcactagga ttaggccact cagcttttgt tgagaagcga
      601 actgtcatat aagaaattaa aaaaatactg aaaacaaaag tatcacttac aagaaagatc
      661 cacatcattg ctttacccca aggaacacct ttaaatgctt tttggtcaga agcccaatcg
      721 gataccatcc ctttattagg aggaattgta acttgaactg attctgtaga ttgttccatt
      781 aatttctcct cattatgaaa ataacattag accaaataag acaacccaaa cagctaataa
      841 aaaatgccag taaattgcac acaattcaac agtgtgttga gtttttacca ctgctacttc
      901 acttggaatc caaacttttt ttactgctat attccagtaa aacaatcctc cgattaggtg
      961 gagaccgtgg agagcagtaa ataaataaaa gtatgcattg gcagggttag ttgaaagata
     1021 gtatcctgaa ctaattagat tttgccaaac catcaattgc cctaaaagaa aaattaaagc
     1081 tagaactcca attagtaaca ttctagattt tactttttca aactctttat ttttagaagc
     1141 aatctgtacg ctaacgaaag ctacactgct aaccaataat ataaatgtat taaaccacaa
     1201 caacagaggt tccggcataa tctgccagtc cggatacacc attcgatctg cataagtgac
     1261 aataaataag caaaagaaaa tcgtacatac aatcatgata aaccgtaagc ctagttttcc
     1321 tttgcttaaa tttattgttt ctcctttgtg aagattatcg agtttagctt gagaaggatc
     1381 ccaaggcttt tgtgtaagaa gttgaaagat actttctttt ttcttcacgt cttctcagct
     1441 tgtgcgccag aaacttcact ggga
//
LOCUS       DTVV01000392            1033 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2154106, whole genome shotgun sequence.
ACCESSION   DTVV01000392 DTVV01000000
VERSION     DTVV01000392.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2154106"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="EYO86_04625"
     CDS             complement(<1..126)
                     /locus_tag="EYO86_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIC42350.1"
                     /translation="MAKFLSDRRDNKNQFITIILTLIVGLIPAILVFGQPDLGTAI"
     gene            134..640
                     /gene="nuoE"
                     /locus_tag="EYO86_04630"
     CDS             134..640
                     /gene="nuoE"
                     /locus_tag="EYO86_04630"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIC42351.1"
                     /translation="MIIEKYPPERQQSAVIPLLDLAQRQNKGWLNKSAIEKIAETLSM
                     SFIRVLEVVTFYSMFNLNPVGKNYVQICRTTPCWLRGSDKLSEVAKEVTGCKIGVTSN
                     DNQFTVVEVECLGACCNAPMVQINDDYYEDLTENNFRDLLNNLKTNKSIKPGSQIGRQ
                     TSDPKRNN"
     gene            642..923
                     /locus_tag="EYO86_04635"
     CDS             642..923
                     /locus_tag="EYO86_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42352.1"
                     /translation="MLKEKDKIFKNIYGYDDWGIEGSRKRGIWSNTKDLIAKGSDYII
                     EEIKKSDLRGRGGAGFPAGLKWSFMPKNSGKPHYLVINADESEPGTCKG"
BASE COUNT          386 a          142 c          200 g          305 t
ORIGIN      
        1 aatagccgtt cctaaatctg gttgaccaaa taccaatatt gcaggaatca agcctacaat
       61 caatgttaat ataatagtta taaattgatt tttattatct cttctatcag ataaaaattt
      121 tgccaaagca caaattatta ttgagaaata tcctccagaa agacagcaaa gtgcagtaat
      181 acctcttctg gatttggctc aaagacaaaa caaaggctgg ttaaataaaa gcgctataga
      241 aaaaatcgca gaaactttaa gtatgtcttt tataagagtt ttagaagtag tcacttttta
      301 ttctatgttt aatttaaacc cagttggtaa aaattatgtt caaatatgca gaactactcc
      361 ttgttggttg cgaggcagtg ataagttatc agaggtggct aaagaagtaa ctggttgtaa
      421 aattggagtt acttctaatg ataatcagtt tactgttgtt gaagtagaat gtttaggagc
      481 ttgttgtaat gcacccatgg ttcaaattaa tgatgattat tatgaggatt taactgaaaa
      541 taattttaga gatttgttaa ataatttaaa aactaacaaa agtattaaac caggttcaca
      601 aataggaaga caaacttcag atccaaaaag aaataactaa tatgctgaaa gaaaaagata
      661 agatttttaa aaatatatat gggtatgatg attggggcat tgaaggatct cgaaaacgtg
      721 gaatatggtc taacactaaa gatttaattg ccaaaggaag tgattacatt atcgaagaaa
      781 ttaaaaaaag cgatttaaga ggtcgtggtg gagcaggctt tcctgctgga ttaaaatggt
      841 cattcatgcc aaaaaattct ggtaaacctc attatcttgt aataaatgca gacgaatcag
      901 aaccaggaac ttgtaaaggg tagccagtcc acacagaatc tacccggata tggcataaag
      961 ttcgtggagg tagaacattc aggatgttcc cagagtagca atgctgaggc acttatagtc
     1021 aaggaactgg tag
//
LOCUS       DTVV01000393            1342 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2156294, whole genome shotgun sequence.
ACCESSION   DTVV01000393 DTVV01000000
VERSION     DTVV01000393.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2156294"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="EYO86_04640"
     CDS             <1..339
                     /locus_tag="EYO86_04640"
                     /inference="COORDINATES: protein motif:HMM:PF02873.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42353.1"
                     /translation="PDFKVEPNVGSFFKNLILDETTFNKLKKKNVDLPHFKNPKDLTY
                     KVPVAFLIENAGWKGKKQGNVRVSEKHALVLIADKGSNSEQLIKLSSVIAKDIYEKTQ
                     VELEVEPKIF"
     gene            339..956
                     /locus_tag="EYO86_04645"
     CDS             339..956
                     /locus_tag="EYO86_04645"
                     /inference="COORDINATES: protein motif:HMM:PF01810.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIC42354.1"
                     /translation="MSIFLTIAMLHLFAVASPGPDFILVSRQCLRYGRRVAIWTSFGI
                     AIGILFHVALSLTGLSILLQNQPDLFWYLKLLASLYIGYLGIVSLVSKSSNKLAEDSI
                     GQAGNQLRSVTTGLLTNVLNPKALIFFITVFALVLNKEIGIIVKSLLGIYMSVATFIW
                     FVFVSILLTNKKGIERFRNIIPLLEKVTGFFLLLIAIQILFQQTY"
     gene            971..>1342
                     /locus_tag="EYO86_04650"
     CDS             971..>1342
                     /locus_tag="EYO86_04650"
                     /inference="COORDINATES: protein motif:HMM:PF06938.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HIC42355.1"
                     /translation="MNKPNPASILKQISNYKDKELPPVHLWNPPLCENVEMKIDREGR
                     WFFMNSPIGRERMVKLFSKVLRLDEDGEYYLVTPVEKIRIEVEERPFLIIDYQLIKKG
                     DKQIISFETNTGDIFLLDKDHP"
BASE COUNT          479 a          186 c          237 g          440 t
ORIGIN      
        1 cctgatttta aggtagaacc taatgtagga agttttttta agaacttaat actagatgag
       61 acgactttta acaaattaaa aaagaagaac gttgatcttc ctcattttaa aaaccctaaa
      121 gatttaactt ataaagttcc agtagctttt ttaattgaaa acgcaggatg gaaaggtaaa
      181 aagcaaggga atgttcgagt gtctgaaaaa catgctttag ttttaattgc ggataaagga
      241 tcaaatagcg aacaattaat aaagctgtct tctgtcattg caaaagatat ttatgaaaag
      301 acacaagtag aactagaagt tgaacctaag attttttaat gtctattttt ttgacaattg
      361 ccatgcttca tttatttgct gttgcaagcc caggcccaga ttttatctta gtcagtagac
      421 aatgcttacg gtatggaaga agagtagcaa tttggactag ttttggaata gctataggaa
      481 ttttatttca tgtagcttta tctcttacgg ggctaagtat acttctacaa aatcaaccag
      541 atctattttg gtaccttaaa ttattagctt ctttatatat tggttattta ggaatagttt
      601 ccctggtttc aaaaagttcc aacaagttag ctgaagattc tataggccaa gctggaaatc
      661 agttaaggtc agtaactaca ggactgctaa caaatgtttt aaatccaaaa gctttaattt
      721 ttttcataac agtctttgct ttggttttaa acaaagaaat aggaataatt gttaagagtt
      781 tattgggaat ctacatgtca gtagcaactt ttatttggtt tgtgtttgta agtattttat
      841 taacaaataa aaaaggaatt gaacggttta gaaatataat tccattactg gaaaaagtta
      901 ctggattttt cttgttatta atagctattc aaatattatt tcaacaaacc tattaggatc
      961 tgagacttct ttgaataaac caaacccagc atcaatactg aaacaaattt caaattacaa
     1021 agataaagaa ttgccgcccg tacatttatg gaatcctcca ctttgtgaga atgttgagat
     1081 gaaaattgac agggaaggta ggtggttttt catgaactct ccgatcggca gagaaagaat
     1141 ggtaaaactt tttagcaagg tcttaaggtt agatgaagat ggagaatatt atctagttac
     1201 cccagttgaa aaaattagaa tagaggtcga agaaagaccg tttcttatta ttgattacca
     1261 gctaataaaa aaaggtgata aacaaataat aagcttcgaa acaaataccg gagatatttt
     1321 ccttttagat aaagaccatc ca
//
LOCUS       DTVV01000394            1751 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2158464, whole genome shotgun sequence.
ACCESSION   DTVV01000394 DTVV01000000
VERSION     DTVV01000394.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2158464"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="EYO86_04655"
     CDS             complement(<1..704)
                     /locus_tag="EYO86_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HIC42356.1"
                     /translation="MISSNYLSVWSLFKKEVFRFFKVGIQTIIGPAVSSLLFLAVFNL
                     ALGRSVQTINGVSFAKFIAPGLIMMTILQNAFANSASSIGQAKSQGNIVDVLMAPLGD
                     FELALGYVGGSVARGLICGLVTAIGMIIFIPLSIYSFAALLFYAIMGSLMMGSLGTMV
                     GIWADKWDQQQGITNFIVLPLTFLSGTFYSISRLPDFWQNFANFNPFFYNIDGFRYAF
                     LGQSDSSLFVGSVTLIT"
     gene            758..1237
                     /locus_tag="EYO86_04660"
     CDS             758..1237
                     /locus_tag="EYO86_04660"
                     /inference="COORDINATES: protein motif:HMM:PF02631.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HIC42357.1"
                     /translation="MDDQMPLMKYAIDYLSKYSSSKNNLERILKKKINRLKIEKKEKL
                     ILYNSINQIMLNLEKNKFIDDNNYAISKIRLFAMQGKSKGFIKSYLIQKGIEKNILNN
                     SLDQFEEEDPEWEKKSARIFVKKKRLENSNENKQKNLSKMARAGFDYDTIKQVLELD"
     gene            complement(1238..>1751)
                     /locus_tag="EYO86_04665"
     CDS             complement(1238..>1751)
                     /locus_tag="EYO86_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIC42358.1"
                     /translation="SKKKTQIWHEIDHSVKKYISTKFIIASATAALFGSTYWFLDLEL
                     ALVFASLTFFLTFIPVFGAVVAVILPIPVAFIQYTSPLPIILIIALPTIFKVIIGDFI
                     EPKVLGSALKLHPVTIVLSLIFWGILWGVVGVFLAVPITAIIKISFQKFETTLPFARL
                     LEGNLHHRRN"
BASE COUNT          719 a          271 c          277 g          484 t
ORIGIN      
        1 gtaatcagtg tgacactgcc aacaaataat gagctatctg attgacctag aaatgcatag
       61 cggaatccat caatgttgta aaaaaaagga ttaaaattgg cgaaattttg ccaaaaatct
      121 ggtaagcggg aaattgaata aaaagtgcca gacaaaaaag tgagaggcaa gacaataaaa
      181 tttgtaattc cttgctgttg atcccattta tcagcccata tcccaaccat tgttcctaaa
      241 gaacccatca tcaaacttcc catgatcgca tagaataaaa gggcagcaaa agaataaatt
      301 gataaaggta taaaaataat cattcctatt gccgttacaa gtccacaaat taaacctcta
      361 gcaacagatc cgccaacata tcccaaagct aattcaaaat ctccaagagg agccatcaaa
      421 acatcaacga tgtttccttg tgattttgct tgcccaatgc ttgacgcaga gttagcgaaa
      481 gcattttgta atatagtcat catgattaaa ccgggggcaa taaatttagc aaaggaaact
      541 ccgttaattg tttgaactga tcgacctaac gcaagattga aaactgctaa aaaaagtaag
      601 gaactgactg ctggaccaat aatggtttga attccaacct taaaaaatcg aaaaacttct
      661 tttttaaaaa gcgaccatac tgatagataa tttgaagaaa tcataaaaat gtttatccta
      721 ttatcttaat atacaacttt taacaatttt aaacctaatg gatgatcaaa tgcctttaat
      781 gaaatatgct attgattatc taagtaaata tagttcaagc aaaaacaacc tagaacgaat
      841 acttaaaaaa aaaataaatc gattgaaaat cgaaaaaaag gaaaagctta tattatataa
      901 ttctatcaat caaattatgc tgaatttaga aaaaaacaaa tttattgatg acaataatta
      961 tgcaatatcc aaaatacgat tgtttgcaat gcaaggaaaa tcaaaaggtt ttataaaaag
     1021 ctatttaata caaaaaggaa ttgaaaagaa tattttgaat aattctttag atcagtttga
     1081 agaagaggat cctgaatggg aaaagaaatc agcaagaatt tttgtaaaga aaaaaaggct
     1141 agaaaattct aatgaaaaca aacaaaaaaa cttaagcaaa atggctagag caggattcga
     1201 ttacgatacg attaagcaag ttttagaact tgattaacta atttcttcga tgatgcaagt
     1261 taccttcaag caatctagcg aatggcaaag ttgtttcaaa tttttgaaat gaaattttga
     1321 taatcgcggt aataggaact gccaaaaaaa cacccacgac tccccataaa attccccaaa
     1381 aaattaaaga taatacaata gtgacaggat gaagttttaa agctgatcca agaactttag
     1441 gttcaataaa gtctccgata ataactttaa atattgtagg caatgctatt attaaaatga
     1501 ttggtagggg acttgtgtat tgaataaaag ctacggggat tggcaaaata actgcaacta
     1561 cagctccgaa aactggaatg aatgttaaaa agaaagttaa acttgcaaaa actagcgcta
     1621 gttctaaatc taaaaaccaa taagtagaac caaataatgc agctgtagct gaagctatga
     1681 taaactttgt cgaaatatat ttttttacac tgtgatcaat ttcatgccaa atttgagttt
     1741 ttttcttgct a
//
LOCUS       DTVV01000395            2366 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2167760, whole genome shotgun sequence.
ACCESSION   DTVV01000395 DTVV01000000
VERSION     DTVV01000395.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2167760"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="EYO86_04670"
     CDS             <1..298
                     /locus_tag="EYO86_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIC42359.1"
                     /translation="EVTCLPNKEAAVVWGKVEVVVYADDYLPLRMIYYDEDLFLSRTL
                     EFTNIEMMDGKMIPTMMNIFPTDEPGESTTVKWEEIQFDITIDDEFFSLRKLQQ"
     gene            316..1536
                     /locus_tag="EYO86_04675"
     CDS             316..1536
                     /locus_tag="EYO86_04675"
                     /inference="COORDINATES: protein motif:HMM:PF02687.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC42360.1"
                     /translation="MKIARISWRSLWRNSTRTNVTIAAVALCIAILIIFQSLIIGLIE
                     KAVYNTTNLVVGEVQIHATGYLDDRSIYKSLKNIEKIRAVAKENNIGMVERSYGFGLI
                     SSGTKSAGTQFWGVDPVSELKYFDFANHIDEGNFLTKKSLKKVVLGQKLARSLAAEVG
                     TELVVFVQGADGSLGNELYYVTGILGNVADNIDRSATIILKSDFDILFSTNNLIHEIA
                     LNSKGKMEAEKIQKLISAKVTDIDVETWKELMPTIAVMTEKMSVFMLTMFSLIFTIAA
                     SLGVMNTLVMSTYDRMKEFGIIRAIGATPWLIIRQVSLEAIFLTFLASIIGTVIGLSI
                     AIYLQVYGIDISGKGNLAIGGIVFDPIWRASVSIKSVFLPIVLMMITSIVASIYPASI
                     AARIKPVEAIHYKP"
     gene            1533..>2366
                     /locus_tag="EYO86_04680"
     CDS             1533..>2366
                     /locus_tag="EYO86_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42361.1"
                     /translation="MMIKFLLILKIAWRSIWRNKRRTFISLFSILIATCVPTFFVCIA
                     WGFYAGLVDDVARLMAGHVTYEHVEYRNSPSNDLWVDDIQTINRILNDKDEVLSTKQI
                     VNLQGVAHTAKGAVGVSLMGIEPSKEIEISFLPESIIAGEYLSQGDGRWVIVGTKLAE
                     QLNIKVGKKFVFTTNDINGEMVEDLFRVKGIFETGSKQIDGHFVQSDISFARKIAGLD
                     NNSTSQLGIIVKNSDIHENLLKEWQNELAKNNGVFLSWQQIMPDIATTIRMDRTAVVS
                     FM"
BASE COUNT          781 a          336 c          492 g          757 t
ORIGIN      
        1 agaggttacg tgtttaccca acaaggaagc tgcggttgtt tggggtaagg ttgaagttgt
       61 agtatatgcc gatgattatc ttccattacg gatgatatat tatgacgagg acctattcct
      121 atcacgtact cttgaattta ctaatattga aatgatggat gggaaaatga ttccaacaat
      181 gatgaatata tttccaacag atgagccggg tgaatcgaca actgtgaaat gggaggaaat
      241 tcaatttgat attacaattg atgatgagtt tttttctttg cggaaactac aacaataatg
      301 gataataaat aacatatgaa aatagcgaga attagttggc gcagtttgtg gcgcaattcc
      361 acaagaacta atgtaacaat agcagctgtt gcattatgta ttgctatatt aattattttt
      421 cagtctttga ttataggact aattgaaaag gcagtataca atacaaccaa ccttgtggtc
      481 ggggaagttc agatacatgc aactggttat cttgatgatc gttccattta caaatcatta
      541 aaaaatatag aaaaaattag agctgttgca aaagaaaata atattggcat ggtggaacga
      601 agctatgggt ttggtttgat ttcatcaggg acaaaatcgg ctggcacgca gttttgggga
      661 gtcgatccag tatcagaact caaatatttt gattttgcca atcatataga tgaagggaat
      721 tttttaacaa aaaaatcttt aaagaaggtt gtattaggcc aaaaattagc tcgttcacta
      781 gcagcagaag tgggaactga attggtagta tttgtccaag gcgctgacgg ctcactaggt
      841 aatgaacttt attatgtaac aggtatctta ggaaatgtag ccgataacat tgataggagc
      901 gcaactatta ttttaaaaag tgattttgat attttatttt ctaccaataa cctaattcat
      961 gaaatcgcat taaattctaa gggaaaaatg gaagctgaaa aaatacaaaa gcttatatca
     1021 gccaaggtta ctgacataga tgtagagacc tggaaagaac tgatgccaac gattgctgta
     1081 atgacagaaa aaatgtctgt ctttatgctg actatgttta gtttaatatt tactattgcc
     1141 gcaagtcttg gagtgatgaa tacattggta atgtctacat atgaccggat gaaagaattt
     1201 ggcatcattc gagctattgg agcaacacct tggttaatta taagacaggt atctttagaa
     1261 gcaatttttt taacgttcct tgcttcaatt attggtacgg tgattggatt gagcatagca
     1321 atatatttgc aagtgtacgg aattgatatt agcggcaaag gaaatcttgc tattggagga
     1381 atagtattcg atccaatttg gagggcgagt gtttctatta aatctgtttt tcttccaatt
     1441 gttttaatga tgataactag cattgtagct tcaatctatc cagcaagcat tgctgctcgg
     1501 attaaacctg tggaggctat tcattataag ccatgatgat aaaattttta ctgattttaa
     1561 aaattgcttg gcgcagcatt tggcgtaaca aaagacgcac atttatttca ttattttcta
     1621 tattgattgc tacgtgcgtt ccaacttttt ttgtatgtat tgcatgggga ttttatgcag
     1681 gacttgttga tgacgtagca cgcctgatgg caggacacgt aacatatgaa catgttgaat
     1741 atcgtaactc accttccaat gacctatggg tagatgatat tcaaacgatt aacaggatat
     1801 taaatgataa agatgaagtt ttatctacaa agcaaatagt aaatttacag ggtgtagctc
     1861 atactgccaa aggtgctgtt ggagtatcct tgatgggaat agaaccatca aaagaaatag
     1921 agatttcttt tttaccggaa tcaattattg caggagaata tttatctcaa ggggacggtc
     1981 ggtgggttat agtaggaacc aaattagcag aacaattaaa tatcaaggtt ggaaaaaaat
     2041 ttgtttttac aacaaatgat attaacggtg aaatggttga ggatttattt cgtgtcaaag
     2101 gcatttttga aacgggatct aaacagattg atggtcattt tgtacaaagt gatatcagtt
     2161 ttgctagaaa gattgcaggc ttagataata attctacttc acagttaggc attattgtaa
     2221 aaaattccga tattcatgaa aatctcctta aagagtggca gaacgaatta gccaaaaaca
     2281 atggtgtttt tttatcatgg cagcaaataa tgcctgatat tgcaacaact attcgtatgg
     2341 atcgcaccgc agttgtttct tttatg
//
LOCUS       DTVV01000396            4019 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2167886, whole genome shotgun sequence.
ACCESSION   DTVV01000396 DTVV01000000
VERSION     DTVV01000396.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2167886"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="EYO86_04685"
     CDS             <1..696
                     /locus_tag="EYO86_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC42362.1"
                     /translation="SHLRDSKNRWFALSKRARIVAVSKDRVPIGAITKIEDLTEPKWK
                     GKICTRSGSHAYNRSLLASIIATHGEEGAEEWAKGLVKNLARKPEGNDRAQAKAIYEG
                     VCDVALMNTYYFGKMKFNEKNPEQKDWAKAINLIFTNQTDRGSHINVTGGGIVKYSKN
                     KENAIDLLEFLTNPKAQMLYSSMNYEYPVNLKVPLSDELKLWGEFKEDELPIEKLAEL
                     APIAQKIINRVGW"
     gene            714..2333
                     /locus_tag="EYO86_04690"
     CDS             714..2333
                     /locus_tag="EYO86_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HIC42363.1"
                     /translation="MSNWNVWSVSVVIIALLIIAPVLAIFYSAFLGDTSLWPHLFSTV
                     LPRYIFNTLILMFGVGSLSLIFGVSTAWVITRYNFFGKNVLEWALLLPAAVPTYIIAY
                     TYTDIFEYAGPFQGMLRDFFGWENAQNYWFPNIRSMGGAILVMSAVLYPYIYLMTRAS
                     LLTTPISFYQTSSIYGRNTFMHVALPLARPGIVAGLALVLMETISDFGTVDYFALDTL
                     TLGVFNVWLGMNSLSGASQISSVLFIFVVMLLTLEYLARRKQRFHEKSSGQNTMPEEE
                     VSPAGKLICILICLVPLILGFIIPVSILLNFVLKGFAIIDFAEIISTTFSSVFLSLSA
                     SIFIMLVSVLMIVVANYKSSSAQRIIIYLSSCGYAFPGTILAVGIVVFVGWLNDLFYF
                     HLSYWAGGFIILIFAYMIRFLAVSSGAIRTGMLRVHPNLMDVSKTMGVGFVTTLRKII
                     IPLIYMNILIGGILVFVDILKELPITLLLRPFNFETLATYVYQYASDEMLEESSFAAI
                     IIIIAGLGPVIFLNSTIKKISKKKESAAPNY"
     gene            complement(2330..3379)
                     /locus_tag="EYO86_04695"
     CDS             complement(2330..3379)
                     /locus_tag="EYO86_04695"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00005.25,HMM:PF08402.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC42364.1"
                     /translation="MLAVKINNLSHSINNQKVLQNINLELEKDKIACILGPSGCGKTT
                     LLKLIAGLEKVTTGDIIINDEIVSSNTMHLKTEKRKIGFLFQDYALFPHLTVKENLNF
                     AISNGKKEKHDIEEIIEIIKLPNSLHKYPHELSGGEQQRVALARSIIAQPGLLLLDEP
                     FSSLDLSLKEEVRDDTLHLLQKSNISVLIVTHDPFEAMFISNKIYIMQKDGKIVQSGS
                     PRQLYNEPINSYVAGFFGETNKFKGMVKNAEVQTPIGKIKTHNNLESKEVVIHVRPQG
                     IKLTQEQTPVNGIKGTVMASKLMGSFSFVHLSVLDHNNEIVHVHSHMPASFIPGQSTA
                     VGIEIDDEQIYVFPA"
     gene            complement(3379..>4019)
                     /locus_tag="EYO86_04700"
     CDS             complement(3379..>4019)
                     /locus_tag="EYO86_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HIC42365.1"
                     /translation="TGTAEYDNIVTIGYKIRDAINIYYNSVGGKNPTANFQWEFVLVD
                     DSQTKNAWCMPGGKIAVYSGILPIAKNNDGIASIMGHEIAHAVARHSAERASRSILMD
                     AGTYAFERLVLGTSLTGYSRDLYGQLRQVGLELPFNRSQESEADYLGLIFMSLAGYNT
                     DETYKVWERMKEDMGGKGPSEFWSTHPSPDNRINKLKEWIPTVNLKYPAVKS"
BASE COUNT         1181 a          690 c          744 g         1404 t
ORIGIN      
        1 tctcatttaa gagatagcaa aaatcgttgg tttgctcttt caaaaagagc aagaatagta
       61 gctgtatcaa aagatagagt cccaattgga gctataacta aaattgaaga tttaactgag
      121 ccaaaatgga aaggtaagat ttgtactagg tcaggaagcc acgcctataa tagatcttta
      181 cttgcttcaa ttattgcaac tcatggtgag gaaggagcag aagaatgggc aaaaggtctt
      241 gttaagaatt tagcaaggaa acctgagggc aatgatagag cacaagctaa agcaatttat
      301 gaaggtgtgt gtgatgttgc tttaatgaat acatattatt ttggcaaaat gaaatttaat
      361 gaaaaaaatc ctgaacaaaa agattgggca aaggcaatta atcttatttt tacaaatcaa
      421 actgacagag gtagccacat taacgttact ggtggtggga tagtgaaata ttcaaaaaat
      481 aaagaaaacg ctattgatct tttggaattt ttaaccaatc ctaaagctca aatgctatac
      541 tcgtcaatga attatgaata tccagtaaat cttaaagtgc ctttaagtga tgagcttaaa
      601 ttatggggag aattcaaaga agatgagttg ccgattgaaa aattagcaga acttgctcca
      661 attgctcaaa aaatcattaa tagggtgggt tggtaaaatg ttaggctgtt aatttgtcta
      721 attggaatgt ttggtcggta tcagtagtaa ttattgcgct tctaataatt gctcctgtat
      781 tagctatttt ttattctgct tttctaggag atacatcttt gtggccccat ttattttcga
      841 cagttttacc acgctatatt tttaataccc ttatacttat gtttggggta gggagtctca
      901 gccttatctt tggtgtgtca actgcttggg taattacgcg gtataatttt tttggaaaaa
      961 atgttttgga atgggcgttg ttactacccg cggcagtacc cacgtatatt atagcttata
     1021 catacactga tatttttgaa tatgcgggcc cttttcaagg aatgcttcgt gatttttttg
     1081 gatgggaaaa cgctcaaaat tattggtttc ctaatatacg ctccatggga ggagccatac
     1141 tagttatgtc tgctgtactg tatccctata tttatttgat gacgcgagca tcccttctta
     1201 caacgccgat ttctttttac caaacaagtt caatttatgg acgtaataca ttcatgcatg
     1261 ttgcccttcc attagcgcgg cccggtattg tggccggttt agcgttagtc ctgatggaaa
     1321 cgatttctga ttttggcacc gttgattatt ttgcacttga tacgctcact ctaggtgttt
     1381 ttaatgtgtg gcttggaatg aatagtttat ctggagcttc ccaaatatca tccgtattat
     1441 ttatttttgt tgttatgctt ttaactcttg aatatttagc gaggaggaaa cagcgttttc
     1501 atgaaaaaag cagcggtcaa aatacaatgc cagaggagga agttagccct gccggcaagt
     1561 taatttgtat tttgatttgt ttagtacctc taattttagg gtttatcatt ccagtaagta
     1621 ttttactcaa ttttgtttta aaaggttttg ctataattga ttttgcagaa ataattagta
     1681 caacattttc aagtgttttt ttgtcgctat ctgcatcaat attcataatg ttagtttctg
     1741 tattgatgat agtggtggca aattataaat ccagttcagc acaacgtata ataatttatc
     1801 tgtcatcttg tgggtatgcg tttcctggga ctattttagc cgttggtata gtcgtgtttg
     1861 ttggatggtt aaatgatctt ttttattttc atttaagtta ttgggctggc ggttttatca
     1921 tattgatatt tgcttatatg ataagatttt tagcagttag tagtggagca atacgaaccg
     1981 gcatgctccg tgtacatccc aatttaatgg atgtgagcaa aacgatgggt gttggttttg
     2041 tgacaacctt aagaaaaatc atcatacctc ttatttatat gaatatatta attgggggaa
     2101 ttttagtttt tgtcgacata ttaaaagaac tgcccatcac tcttttattg cgtccattta
     2161 attttgaaac acttgcaact tatgtttacc aatatgcatc ggatgaaatg ctagaagaat
     2221 cttcttttgc cgccatcatc attattattg ctggactagg ccccgttatt tttttaaatt
     2281 caacaattaa aaaaattagc aaaaagaaag aaagtgctgc tcctaattat taagctggaa
     2341 atacataaat ttgttcatca tcaatctcga ttccaactgc tgttgactga ccgggtataa
     2401 aggatgcggg catatgactg tgcacatgca caatttcatt attatgatcc aaaacagaca
     2461 gatgaacgaa actaaaggat cccattaatt ttgatgccat aacagttcct ttgatcccat
     2521 taacaggtgt ttgttcttgg gttaacttaa taccttgtgg ccttacatga attaccactt
     2581 ctttagactc taagttatta tgagttttaa tcttgcctat gggtgtttgc acttcagcat
     2641 ttttaaccat acctttaaac ttatttgttt caccaaaaaa acctgcaaca taggaattga
     2701 taggttcatt gtataattgc cgtggtgagc ctgactgaac aattttaccg tctttttgca
     2761 taatataaat tttgtttgaa atgaacatgg cctcaaaagg atcgtgcgtt actataagaa
     2821 cggatatgtt cgatttttga agcagatgca aagtgtcatc tcttacctct tctttaagac
     2881 taagatccaa actagaaaac ggttcatcga gtaataataa acctggttga gcaattatgg
     2941 aacgtgcaag agccactcgt tgttgctccc ctccacttaa ttcgtgagga tatttgtgga
     3001 gtgaattagg taacttgatt atttcgataa tttcctcaat gtcatgtttt tctttcttac
     3061 cattactaat tgcaaaattt aaattttcct tcaccgttaa gtgtgggaac agggcatagt
     3121 cttggaataa aaaacctatt tttctctttt cagttttaag atgcattgta ttagagctga
     3181 caatttcatc atttattatg atatctcccg ttgtaacttt ctccaaaccg gcaattaatt
     3241 ttaaaagggt agttttaccg caacctgaag gaccgagaat acaagctatc ttatcttttt
     3301 ctaattcaag attaatattt tgtaatactt tctgattatt aattgagtgg cttaaattgt
     3361 ttattttaac agctaacatt atgatttgac tgcaggatat ttaagattaa cggttggtat
     3421 ccactcctta agtttattga tgcgattatc aggtgaggga tgagtgctcc aaaattcaga
     3481 cggaccttta ccacccatat cttctttcat tcgctcccac actttatatg tttcatctgt
     3541 gttataccca gctaaactca taaaaattaa tcctagataa tctgcttccg attcctgact
     3601 tcgattaaaa ggaagttcta atccaacttg acgaagctgg ccatataaat cacgtgaata
     3661 tcctgtgaga ctagtgccca gcaccaatct ttcaaaggca taagtaccag cgtccattag
     3721 aattgacctg cttgctctct cagcagagtg ccgcgctact gcatgagcta tttcatgtcc
     3781 cattatagag gcaatcccat cattattttt tgcgatcgga agtatgcctg aataaacagc
     3841 aattttacct cctggcatgc accacgcgtt ttttgtttga ctgtcgtcga caagtacaaa
     3901 ttcccattga aagtttgccg taggattttt cccccccaca ctgttatagt atatgttgat
     3961 agcatcacga atcttatatc caattgtaac aatattatcg tattctgccg ttcctgtaa
//
LOCUS       DTVV01000397            1948 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2176801, whole genome shotgun sequence.
ACCESSION   DTVV01000397 DTVV01000000
VERSION     DTVV01000397.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2176801"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            99..1550
                     /gene="sufB"
                     /locus_tag="EYO86_04705"
     CDS             99..1550
                     /gene="sufB"
                     /locus_tag="EYO86_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIC42366.1"
                     /translation="MSIDIQTKKTFKSATEKYKYGFITDIESDRAPKGLNESIVRLIS
                     SKKKEPEWLLDWRLRSYNAWKKMSEPKWPNVKYPKIDYQNSYYYAAPKSKKKLKSLDE
                     VDPAILSTYEKLGIPLEEQKRLTGVAVDYVFDSTSVATTFKEKLSELGIIFCSFSDAV
                     HSHPDLVKKYLGSVIPYTDNYFATLNSAVFSDGSFVYIPAGVKCPMELSTYFRINAEN
                     TGQFERTLIIADKNSYVSYLEGCTAPMRDENQLHAACVELITLDNAEIKYSTVQNWYP
                     GDENGKGGVYNFVTKRGACRGNNSKISWTQIETGSAITWKYPSCILQGDNSTGKFYSV
                     ALTNNFQQADTGTKMLHLGKNTKSTIISKGISAGKAQNTYRGLVKMGPRAINGRNFTQ
                     CDSLLIGDKCGAHTVPYIEVKNKSAQVEHEATTSKISDDQMFYCRQRGLDQENAVSLI
                     VNGFCKEVLKELPMEFAVEAQKLLDISLEGSIG"
     gene            1563..>1948
                     /locus_tag="EYO86_04710"
     CDS             1563..>1948
                     /locus_tag="EYO86_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC42367.1"
                     /translation="MLKIKDLHVKVENKKVLQGLNLEILPGEVHALMGPNGSGKSTLA
                     HILAGKDGYKITKGEVYYQKKNLFDLKPEERACEGLFLAFQYPVEIPGVSGINFLRSA
                     LNTIRRFKKQKELDGINFLKLLRKKT"
BASE COUNT          741 a          278 c          351 g          578 t
ORIGIN      
        1 caaatttatt aaagaaattt aatttagaat cattctaaat taaattgaca tcgctatcta
       61 gtcctttata tattatataa gtatataaaa ttattcatat gtctatagac attcaaacaa
      121 aaaaaacatt taaatctgca actgaaaagt ataagtacgg ttttataaca gatattgaat
      181 ctgatagagc tccaaaagga ctgaatgaaa gcattgttcg tttaatttct tcgaagaaaa
      241 aggaacctga atggctttta gattggagat taagatctta caatgcctgg aaaaaaatga
      301 gtgaaccgaa atggccaaat gtaaagtatc caaaaattga ttatcagaat tcttattact
      361 atgcggctcc aaaaagtaaa aaaaaattaa aaagtctaga cgaagttgat ccagcaattt
      421 taagcaccta cgaaaaatta ggcattccat tagaagaaca aaaaagatta acaggggttg
      481 ccgtggacta tgtctttgat agtacttcag tagcaacaac gtttaaagaa aaattgtctg
      541 aactgggaat aattttctgt tcattttcgg atgcagtcca ttcgcatcct gatttagtaa
      601 aaaaatatct aggatctgtt atcccctaca ctgataatta ttttgccaca ctgaattcag
      661 ctgtatttag cgacggctca tttgtttaca taccggctgg agtaaaatgt ccaatggaat
      721 tatcaactta ttttagaatt aatgctgaaa atactgggca atttgaaaga acactaatca
      781 tcgctgacaa aaatagttat gtcagctacc tagaaggttg tactgctcca atgcgtgatg
      841 aaaatcaatt acatgcagct tgtgtagaat taattacgct tgataacgca gaaattaaat
      901 attcaacagt tcaaaattgg tacccaggtg acgaaaacgg taaaggcggg gtttataatt
      961 ttgttacaaa aagaggagca tgtagaggaa ataactcaaa aatttcatgg acgcaaattg
     1021 aaaccggatc tgccattaca tggaaatacc ctagctgtat tttacaaggg gataattcga
     1081 caggtaagtt ttattctgtt gctttaacga ataattttca acaagcagat acaggaacta
     1141 aaatgctgca cctcggtaaa aatacgaaaa gtacaattat ttctaaaggt atctcagcag
     1201 gtaaggctca aaatacttat agaggcttgg tcaaaatggg accaagggca ataaatggta
     1261 gaaattttac acaatgcgat tctttactta taggtgataa atgtggagca catactgttc
     1321 cttatataga agtaaaaaat aaatctgcgc aagttgaaca tgaagcaacg acatccaaaa
     1381 ttagtgatga tcaaatgttt tactgccgac aaagaggttt ggatcaagaa aacgctgtat
     1441 ctttaattgt aaatggtttt tgtaaggaag tgcttaaaga attacctatg gaatttgctg
     1501 tagaggcgca aaaactttta gatatcagtc ttgaaggaag tattggataa atttttattt
     1561 ctatgctcaa aatcaaagat ctccatgtaa aagtcgagaa taaaaaagta ttacaaggtc
     1621 ttaatcttga aatactacct ggcgaggttc atgccttaat gggaccaaat ggttctggta
     1681 aaagtacttt agcacatatc ttagcaggaa aagatgggta taaaattacc aaaggagaag
     1741 tttattatca aaaaaaaaat ttatttgatt tgaaaccaga agaaagagca tgtgaagggt
     1801 tatttttagc gtttcaatat cctgtagaaa ttcctggtgt ttctggaata aattttttaa
     1861 gaagtgcatt aaatactata aggaggttca aaaaacaaaa agaactcgac ggaataaatt
     1921 ttcttaaact tttaagaaaa aaaacaaa
//
LOCUS       DTVV01000398            1831 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2179845, whole genome shotgun sequence.
ACCESSION   DTVV01000398 DTVV01000000
VERSION     DTVV01000398.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2179845"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="EYO86_04715"
     CDS             complement(<1..360)
                     /locus_tag="EYO86_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HIC42368.1"
                     /translation="MNKNLLGKFIHLKKLCSEFYHFQMLAVDQRPPIFKIISAAKKRK
                     HSYKEVVECKNLISSNLSPLASATLLDPHYSIPNILKHNKSKGLIITLEDHEFRETKK
                     GRYSKNIKNWTVEKIKKI"
     gene            647..1549
                     /locus_tag="EYO86_04720"
                     /pseudo
     CDS             647..1549
                     /locus_tag="EYO86_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1542..1831
                     /locus_tag="EYO86_04725"
                     /pseudo
     CDS             1542..1831
                     /locus_tag="EYO86_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250637.1"
                     /note="catalyzes the synthesis of xanthosine monophosphate
                     by the NAD+ dependent oxidation of inosine monophosphate;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
BASE COUNT          565 a          299 c          384 g          583 t
ORIGIN      
        1 aattttttta attttttcta cagtccaatt tttaatattt ttcgagtatc tacccttttt
       61 cgtttctctg aattcatgat cttccaaagt aatgattaaa cctttagatt tattatgttt
      121 tagaatattg ggaatagagt aatgaggatc caaaagagtt gctgaagcca atggtgataa
      181 gttagaagaa attaaatttt tacactcaac tacttcttta taagaatgct ttcttttttt
      241 cgcggcagag atgattttaa agatgggagg cctttgatca acagctaaca tttgaaaatg
      301 ataaaattca ctgcaaagtt tttttaaatg tatgaatttg ccaagtaagt ttttattcat
      361 gaaaaaattt ctattttaat attttgtttc ctgttattga ttttttaatc aatttttacc
      421 cttttttgat ctttcatttt tttcccttac tatattccaa taaaattcat aaacaaaaat
      481 ataagctttc gaacataatt atactatgaa cgaacacgga tattgtgtag tataccggga
      541 atgagaaaaa acgaaaattt tttttttatt ttcggattag tttcaaaaca caattgtaaa
      601 ctataaatta attcatgaac tatttattaa atttgaagga gaatctatga ataaaatttt
      661 taaagctttc atagctttgg taactttagt tttcctgtct ttaccagtga atgcggctcc
      721 aattgagctt cactattggc atggacacac tggtaaacta gagactatca ttaatgaaat
      781 agctgagaga tggaatgcaa gtcaagatga gtctaaatta attcctacta gtaaaggaag
      841 ttacgaggat atcctagcgg cttttatcgc tgcatacaga gctggtgaac atcctcatat
      901 ggttcaagtc tatgatgcag gtgcggccat tatgattgcg caggtaaaaa atggagttgt
      961 atatccgttt gaagatatgg cagacaagta tggtgtagat ttcaataaag atgattacat
     1021 tccagggatc agaaatttct atgcagactc agatggaaaa atgattggca ttccttttaa
     1081 cagctcaact tgtttaatgt acgctaacat ggacatttta gaagcagctg gtgttgaagt
     1141 gccaaaaact tatgaagagt ttgaaggtat tgctcaaaaa attaaggatg ccggatacat
     1201 accattgtta caaagccata gcccttggat ttggactgaa aacttctttt caagacataa
     1261 tcttttaatg actgatggaa ataatggtta tgatgctgtc ccaacaacaa cacttttcgc
     1321 tgacactcca gagtttatca ctcactggga aaaagttaaa gagtggaaag ataaaggatg
     1381 gtatgattac aaaggacgtg cgtggggtga caaccaagct cctttcattg caggcgaagc
     1441 agcattttgg cttggctcag cagcatcatt tagtggtatg aagggtgttg ttccaaattt
     1501 ttctgttaat cacattcctt attggagctc aatgactggt ggaaaacaag gtcgagaaac
     1561 tgttgttagt ggacgaagat taccgattaa aagggttaat cacgatcaag gacatcgaca
     1621 agaacctccg cttcccgctg gcgtcgaagg acgaacgcgg gcggttgcga gtcggggccg
     1681 cggttggcgt cctcgacttt gaccgggcgg agagcctgat cgagacgggc gtcgatgtcc
     1741 tggtcgtcga cagtgctcac gggcattcgc ggaacgtgat cgagacggtc aaggagatca
     1801 agcggcgaag tgacatcgac gtcgtggcgg g
//
LOCUS       DTVV01000399            1197 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2186606, whole genome shotgun sequence.
ACCESSION   DTVV01000399 DTVV01000000
VERSION     DTVV01000399.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2186606"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(4..834)
                     /gene="thyA"
                     /locus_tag="EYO86_04730"
     CDS             complement(4..834)
                     /gene="thyA"
                     /locus_tag="EYO86_04730"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIC42369.1"
                     /translation="MKQYLKALRYCYNNGIDVESRAGKVRKAFGYQMRFDLQKGFPAM
                     TTKKLAWKVVVSELLWFLEGSNDERRLAEILYNDKRENLIDKKTIWSQNANSVYWKKQ
                     SKFSGDVGRIYGVQWRDFNGIDQITSLIEGIKKDPNGRRHILSAWNPVDLPKMSLPPC
                     HAFSQFFVSQNKLSCQLYQRSCDMFLGVPFNIASYSLLVHLISKECNLEVGEFVLTLG
                     DYHIYHEHFEAVNIQLERQPRNLPHLQFIQKNIFIYSVNDFELTNYNPYPTIKAAMNV
                     "
     gene            complement(831..>1197)
                     /locus_tag="EYO86_04735"
     CDS             complement(831..>1197)
                     /locus_tag="EYO86_04735"
                     /inference="COORDINATES: protein motif:HMM:PF00186.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42370.1"
                     /translation="IMGRLTWIDPFLPTPLKNRINVLATNQSHNTYLGVDEFISGDLM
                     ANVNDLSKKYKEKDIYIIGGPKILDQLFESIEEFYLTRIYGNFECDKSIDFEKITQSM
                     TINEKIENDETCHFEIWKR"
BASE COUNT          389 a          219 c          166 g          423 t
ORIGIN      
        1 taatcaaaca ttcatagccg ctttaatggt gggataggga ttgtaatttg ttaattcaaa
       61 atcgttaact gaatatataa aaatattttt ttgtataaat tgaaggtgag gtaagttacg
      121 aggttgcctc tctaattgaa tgtttacagc ttcaaaatgt tcatgataaa tatgataatc
      181 accaagagta agaacaaatt cccccacttc taaattacat tctttggaaa taaggtgaac
      241 gagtaaactg taacttgcaa tgttaaaggg aactcccaaa aacatatcgc aagaacgttg
      301 gtataactga cagcttaact tgttctgtga cacaaaaaac tgggaaaaag catgacaagg
      361 cggcaaagac atttttggaa ggtcgactgg attccaagct gacaaaatat gtctccttcc
      421 attaggatct tttttaatac cctcaattaa gcttgtaatt tgatctattc cattaaaatc
      481 tcgccattga acgccatata ttcttcccac atccccacta aactttgact gttttttcca
      541 ataaacagaa ttagcatttt gtgaccatat tgtttttttg tctataagat tttcacgttt
      601 atcattatac aaaatttctg ctaaacgacg ctcatcattt gaaccttcta aaaaccaaag
      661 taattctgaa acaactacct tccaagcaag tttttttgtt gtcatagccg gaaatccttt
      721 ctgtaaatca aagcgcattt gatacccaaa ggcttttctt actttccccg cacgactttc
      781 aacatcaatc ccgttattgt aacaatatct aagagctttt aaatactgtt tcatcttttc
      841 caaatttcaa aatggcaagt ttcatcattt tcaatttttt catttattgt catagactgt
      901 gttatttttt caaaatcaat acttttatcg cattcaaagt ttccatatat tcttgttaaa
      961 taaaactctt ctattgactc aaatagttga tcaagaattt ttggacctcc aatgatataa
     1021 atatcctttt ctttgtattt tttcgataaa tcattaacat tggccattaa atctcccgat
     1081 ataaactcat caacaccgag ataagtattg tggctttgat tagttgctaa tacattaatg
     1141 cgatttttta gtggagttgg caaaaaaggg tctatccagg ttaatcttcc cattata
//
LOCUS       DTVV01000400            1041 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2188607, whole genome shotgun sequence.
ACCESSION   DTVV01000400 DTVV01000000
VERSION     DTVV01000400.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2188607"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="EYO86_04740"
     CDS             <1..378
                     /locus_tag="EYO86_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020057784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIC42371.1"
                     /translation="MQVETQKIMNEDIKVSATCVRVPVFIGHGEAINIEFEKEISEDK
                     VRKTLKKSSGISVVDYRQDEGYVTPEECTGEDKVFVSRIRKDNTLKNGISLWIVADNL
                     RKGAALNAIQIGEELIKKKILKK"
     gene            375..>1041
                     /locus_tag="EYO86_04745"
     CDS             375..>1041
                     /locus_tag="EYO86_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42372.1"
                     /translation="MKNKTNSGNFFEDFFVGQELKHSTPRTISSGDVSLFIGLLGSRF
                     VLHSSKEFAKKIGFKEAPIDDILIFNMVFGKTVPDISLNSPANLGYASCKFIQPVYVG
                     DTIKTTSKVLGVKENSNKKTGIVWVNSVGKNQKNEVVLDYIRWVMVNKKKEISPAPKT
                     VIPKLKDEVSIEEVSIPEKLNILNYDFDESGSKYLWDDYQIGEKIDHIDGMTVEEADH
                     MTAT"
BASE COUNT          423 a          106 c          194 g          318 t
ORIGIN      
        1 atgcaagttg aaacgcaaaa aattatgaat gaagatatta aagtttctgc tacatgtgtg
       61 agagttccag tgtttattgg acatggagag gctataaata ttgaatttga aaaagaaatt
      121 tcagaagata aagtaagaaa aacgttaaag aaaagctcag gtatttctgt tgttgattat
      181 agacaagatg agggatatgt aacaccagag gaatgtacag gagaagataa ggtttttgtt
      241 agtagaataa gaaaagataa tacattaaaa aacggtatat ctttgtggat agtagcagat
      301 aatttaagaa aaggggctgc cttaaatgca atacaaatag gtgaagaact aataaagaag
      361 aaaattttaa aaaaatgaaa aataaaacaa attctggaaa tttttttgaa gatttttttg
      421 ttggtcagga attgaaacat tctactccaa gaacaatttc ttcaggcgat gtttctttat
      481 ttataggatt acttggatca agatttgttt tacattcatc gaaagaattt gcaaaaaaaa
      541 ttggatttaa ggaagcaccc attgacgata tattaatttt taatatggtt tttggtaaga
      601 ctgtacctga tatatctctt aattcacctg ctaatttagg atatgcttca tgtaaattta
      661 tccaaccagt ttatgtggga gatacaatta aaactacttc aaaagttcta ggagtaaaag
      721 aaaattctaa taaaaaaaca ggtatagttt gggtaaattc agttggtaag aatcaaaaaa
      781 acgaagttgt tcttgattat ataagatggg ttatggttaa caaaaaaaaa gaaattagtc
      841 ctgctcctaa aacagttata ccaaaattaa aagatgaagt aagcatagaa gaagttagca
      901 ttcctgaaaa attaaatata ttaaactatg attttgatga aagtggttca aaatatttat
      961 gggatgatta tcaaattgga gaaaaaattg atcatataga cggaatgact gttgaagaag
     1021 cagaccacat gactgctaca a
//
LOCUS       DTVV01000401            3510 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2189400, whole genome shotgun sequence.
ACCESSION   DTVV01000401 DTVV01000000
VERSION     DTVV01000401.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2189400"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="sucD"
                     /locus_tag="EYO86_04750"
     CDS             complement(<1..803)
                     /gene="sucD"
                     /locus_tag="EYO86_04750"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017443052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIC42373.1"
                     /translation="MSILINKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GKIHLNLPVFNTVHEAVDATQADATVIYVPAKFATKAIHEALDAKIKIITCITEGIPV
                     LDMVEIKKRIIKQKIFFIGPNCPGLVTPEECKIGIMPGFIHKKGKIGIVSRSGTLTYE
                     AIWQTSQVGLGQTTCVGIGGDPVQGMNFIDCLELFLKDVETEGILMIGEIGGTAEEDA
                     AEFLAQSSIKKPVSAFIAGLSAPKGKRMGHAGAIISGNKGTAQSKIHALV"
     gene            complement(806..1990)
                     /gene="sucC"
                     /locus_tag="EYO86_04755"
     CDS             complement(806..1990)
                     /gene="sucC"
                     /locus_tag="EYO86_04755"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIC42374.1"
                     /translation="MNLHEYQAKELLRQFNLPLLVGKAYINQLDNVDADLDNLQGPPW
                     VVKSQIHAGGRGAGYFKNAFNDQGGVQLVTKKSDVTLISQSMMGNILITKQTGDEGKK
                     VNRIFIEEGCQVDREFYLSLLIDRNSSQLMMMISSAGGMDIEDVARTHPGKIHNIYFP
                     DLQKITLQNSLEKKLEINSNQFNQLFDITKKLVKAFVSLDASTIEINPLVLNKVGDFV
                     LLDAKLSLDDNALFRHPELESLKDITEENPLELEAAQNNMNYVKLDGSIGCMVNGAGL
                     AMATMDIIKQFGQEPANFLDLGGTANKDRAIKGFKIIQSDPNVKSVLINIFGGIIHCD
                     MIANGIVSAIKELDFKLPIVVRFQGTNASQGRDIINKSFSNLTSIDDLTKAAQKVVEL
                     AK"
     gene            complement(1993..2952)
                     /gene="mdh"
                     /locus_tag="EYO86_04760"
     CDS             complement(1993..2952)
                     /gene="mdh"
                     /locus_tag="EYO86_04760"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIC42375.1"
                     /translation="MRKKIALVGAGNIGGTLAHLIGLKELGDIILIDVADGMPQGKAL
                     DLVQSSTIESFNASIKGSTNYKEMKNADVVIVTAGFQRRPGMTRDDLVEKNSSIVQQV
                     GKGIKKYCPKAFVICITNPLDAMVSILQKHAGLKTNMCVGMAGVLDSARLKYFLSNEF
                     GVSVQDVNAFVLGGHGDTMVPLIRYSTVSGIPVPELIKLKWTTQKKIDAIIERTRDGG
                     GEIGRLMKTASAFYAPASSAIAMAESYLKDNKRVLPCAAYLNGEYGVKGLYVGVPVVI
                     GKKGVEKIVELKLNSTEKKQFNNSVKAVRNLTNLAAKLIKKNK"
     gene            complement(2973..>3510)
                     /locus_tag="EYO86_04765"
     CDS             complement(2973..>3510)
                     /locus_tag="EYO86_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIC42376.1"
                     /translation="CAMNIDGVNTLACIKPMSEVKNDIKIYPLPHMRVVKDLVPDLTE
                     AYKQLESIKPWLQKDEDTPSISNTNPIEEKKQNPKERAKLDGSWECVLCFCCTTSCPS
                     YWWNGDKYLGPAVLLQASRWINDSRDEKRKERLEEINDSLKLYRCHSIMNCTRSCPKG
                     LNPAKAIADIKKSIVTEK"
BASE COUNT         1006 a          715 c          570 g         1219 t
ORIGIN      
        1 accaaagcat gaatttttga ttgggcggta cccttgttgc cagaaataat ggctccggca
       61 tgacccattc ttttgccttt tggagcagac aaaccagcga taaatgctga cacaggtttt
      121 ttaatcgatg attgtgctaa aaattcagcc gcatcttctt ctgcagttcc accaatttca
      181 cctatcatta aaattccttc cgtctcaaca tctttcaaaa ataattccaa acagtcaata
      241 aaattcatgc cctggacagg atcgccaccg atgccaacac aagttgtttg acccaatcct
      301 acttgggatg tttgccaaat agcctcgtaa gtaagagttc cagacctgct tactattcca
      361 atttttcctt ttttatgaat aaatcctggc attattccta ttttacactc ttctggtgtt
      421 accaaacctg gacaattagg gccaataaaa aaaattttct gtttaatgat tctttttttt
      481 atttctacca tatccaaaac tggaattcct tcagtaatgc atgtaataat ttttattttg
      541 gcatctaagg cttcatgaat agcctttgtt gcaaatttag ctggaacata aatcacagtg
      601 gcatcggcct gagttgcatc cactgcttca tgtactgtat taaaaactgg tagatttaaa
      661 tgtatcttcc cacctttgcc tggagttact ccaccaacca ttttggttcc gtacgcaatt
      721 gcttgctctg agtgaaacgt tccttgggat cctgtaaacc cttggcaaat tactttcgta
      781 tttttgttta tcaatataga cattgttatt ttgctaactc gacaactttt tgtgctgctt
      841 ttgtaagatc gtcaattgag gttaaattag aaaatgactt atttattata tctcttcctt
      901 gagaggcatt ggttccttga aaacgaacaa caataggcaa tttaaaatct agttctttaa
      961 tagcagaaac aatgccatta gcgatcatat cacaatgaat gatgccacca aaaatattga
     1021 ttaacactga ctttacattg ggatcagatt gaatgatttt aaatcctttg attgccctat
     1081 ctttgttggc tgttccccct aggtctagga aatttgctgg ttcttggcca aactgcttaa
     1141 taatatccat tgtggccatt gctaaaccag cgccattaac catacagcct attgaaccat
     1201 ccagttttac ataattcata ttattttgtg cagcctccaa ctctaatggg ttttcctctg
     1261 ttatgtcttt aagactttcc agctcaggat gacggaatag tgcgttatca tctagactga
     1321 gtttggcatc tagtagaaca aagtcgccta ctttattaag aaccaaagga tttatttcaa
     1381 tagttgaggc atctaaagaa acaaaagctt tgactaattt ttttgtaatg tcaaaaagct
     1441 gattaaattg attggaattg atttctaatt ttttttctaa agaattttga agtgtgattt
     1501 tttgtaaatc aggaaaataa atattatgaa ttttccctgg atgtgttctg gcaacatctt
     1561 ctatatccat accgcctgct gatgaaatca tcatcatcag ctgggacgag ttacggtcaa
     1621 ttaacagact taaataaaat tctctatcta cctggcatcc ttcttcaata aaaatacggt
     1681 taactttttt accctcatct cctgtttgtt ttgtgatcaa aatattgccc atcatggatt
     1741 gggagattaa agttacatct gatttttttg tcactaattg aacgccacct tgatcattaa
     1801 aggcattttt aaaatatccc gcgcctcttc cgcctgcatg tatttgtgat tttaccaccc
     1861 aaggaggtcc ttgcaaatta tcaaggtcag catccacatt atctaactga ttgatgtagg
     1921 ctttacccac tagtaaaggg agattgaatt gtcgtaagag ttcctttgct tggtactcgt
     1981 gaagattcat tattatttat tttttttaat taattttgct gcaagattgg ttaaatttcg
     2041 tactgctttc actgaattat tgaattgctt tttctctgtt gagtttaatt ttaattctac
     2101 aattttttca acaccctttt tgccaattac tactggaaca ccaacgtaca aacccttaac
     2161 gccatactca ccgttaagat aagccgcaca aggtaaaacc cttttattat cttttagata
     2221 cgactctgcc atagcgatag ctgatgatgc aggagcataa aaagccgaag cggttttcat
     2281 taggcggcca atttcaccgc ctccgtcacg tgttctctca ataatagcat caattttttt
     2341 ttgagtagtc catttaagtt taattaattc tggcactgga atacctgata cagttgagta
     2401 acgaataaga gggaccattg tgtcaccgtg tcctcctaaa acaaaagcat ttacatcctg
     2461 aacagaaaca ccaaactcat tagataaaaa atattttagg cgagcactat ccagtacacc
     2521 ggccatcccc acacacatgt ttgtttttaa accagcgtgt ttttgtaaaa tactcaccat
     2581 ggcatccaga ggattagtga tacaaatgac aaatgcttta ggacaatatt ttttaatacc
     2641 ttttcctact tgttggacaa ttgatgagtt tttttctact aaatcatcac gcgtcatccc
     2701 tggtcgacgc tgaaatcctg ctgttacaat aacaacatcg gcatttttca tctctttata
     2761 attcgtggaa ccttttatgc tagcattaaa agattcaatt gttgaggatt gaacaaggtc
     2821 tagcgccttt ccttgaggca taccgtccgc tacgtcaata agtataatgt ctcctaattc
     2881 ttttaaacca atcaaatgtg cgagagttcc gccaatattt cctgctccga cgagagcaat
     2941 tttttttctc atgttgcttc tatatataag aattattttt ctgtaacaat agacttctta
     3001 atatcagcaa tagcttttgc agggtttaag cctttgggac acgaacgagt gcaattcatt
     3061 atagaatggc aacgatataa ttttaacgag tcattaattt cttctagtcg ctcttttcgt
     3121 ttttcatctc gactgtcatt gatccaacgc gaagcttgta ataaaactgc aggcccaaga
     3181 tatttatctc cattccacca ataactagga catgatgtag tacagcaaaa gcacagtaca
     3241 cactcccagc tgccatcaag ttttgctctt tcctttggat tttgtttttt ttcctcaata
     3301 gggttggtgt ttgaaataga cggtgtatct tcatctttct gcaaccatgg tttgatggat
     3361 tctaattgct tgtaggcttc tgttaagtct ggaacaagat ccttcacaac acgcatatgt
     3421 ggtaaaggat aaattttaat atcgttcttt acttcactca ttggttttat gcatgcaagt
     3481 gtattcacgc catcaatatt cattgcacat
//
LOCUS       DTVV01000402            1533 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2191013, whole genome shotgun sequence.
ACCESSION   DTVV01000402 DTVV01000000
VERSION     DTVV01000402.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2191013"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(184..465)
                     /locus_tag="EYO86_04770"
     CDS             complement(184..465)
                     /locus_tag="EYO86_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase F0F1"
                     /protein_id="HIC42377.1"
                     /translation="MNNNKKLEKLKQKLDKLQSSKTDQSHKHKETGAGFGFKISTEII
                     AALVVGVGIGLIVDNYFNTKPFGLIIFFILGALAGFLNVYRVMRRIVKR"
     gene            complement(458..>1533)
                     /locus_tag="EYO86_04775"
     CDS             complement(458..>1533)
                     /locus_tag="EYO86_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HIC42378.1"
                     /translation="QRIDELKERESNLTNALSNLRSVPNNITLEKIRLQKLINENKSA
                     YDKIADKLRETEQNATEINKKLKLVEIKLNEFREEKIRIEGSISIINEAIKQLGAQVK
                     ERVNTQLEELFDLAELNPKKPLAELDDLEKRLERLIAEQERLGGVNLLAEEEAKELEE
                     KVNNIKKNQSDLLAAIAKLREAIDTLNTEGRQRLLAAYGIVNENFQKLFTQLFGGGKA
                     YLKFTDESDPLQAGLEIYASPPGKKLQNLSLLSGGEQALTALSLLFAVFLSNPAPICV
                     LDEVDAPLDDTNVDRFCTLVEEIARTSATKFLVITHHRMTMARVDRLFGVTMPEQGVS
                     QLVSVDLEKAIELRENDELIENE"
BASE COUNT          459 a          271 c          228 g          575 t
ORIGIN      
        1 aaaaaagagt tataaatgct actgtaataa ccattgctag agaagagtta gtgaaagaga
       61 tatcataacc accaatttct gtatgagcaa aagggataat ttcgaattgt tctagtgggc
      121 tgtgtttctc gctatgtcct gaataatctg aagccataaa atttattatt gaataagtaa
      181 ttcctagcgt tttacaatgc gacgcattac acgatatacg ttcaaaaatc cagctaatgc
      241 gccaagaata aagaaaataa taagaccaaa aggtttagta ttaaaataat tatccacaat
      301 aagaccaata cctacaccta ccacaagcgc agcaattatt tcagtactga ttttaaaacc
      361 aaacccagct ccagtttctt tatgtttgtg actctgatct gtttttgaag attgaagttt
      421 gtctaatttt tgttttagct tctctaattt tttattgtta ttcattttct attaattcat
      481 cattttctct taattcaatt gctttttcta aatcaactga aacaagctga gagactcctt
      541 gttcaggcat tgttacacca aataatctat ctacacgtgc cattgtcata cggtggtgtg
      601 taataactaa aaactttgta gctgatgttc tagcaatttc ctctacaagt gtgcagaaac
      661 gatcaacatt tgtatcatct agtggtgcat ctacctcatc taatacacaa ataggagcag
      721 ggtttgataa aaaaactgca aacaaaagag aaagagcagt taaagcttgt tcaccaccag
      781 aaagcagtga taaattttgt aattttttgc ctggtgggct tgcataaatt tccaatccag
      841 cttgcagtgg atcagattca tcagtaaatt ttagataagc tttgcctcca ccaaaaagct
      901 gtgtgaaaag tttttggaaa ttttcattaa caatcccata tgctgctagt aaacgttgtc
      961 ttccttctgt gttcaaagta tctatggcct ctcgtagttt tgcaattgct gctaataaat
     1021 cactttgatt tttttttatg ttattaactt tttcttcaag ttcttttgct tcctcttctg
     1081 caagtaaatt gacaccacct aatcgttctt gttcagctat caagcgctct aaacgctttt
     1141 ccaaatcatc tagttcagca agtggttttt ttggatttaa ctcagcgaga tcaaataatt
     1201 cttctaattg tgtatttaca cgttctttta cttgtgctcc aagttgtttt attgcttcat
     1261 tgataatact gattgaacct tctattcgaa ttttttcttc acgaaattca ttcaatttta
     1321 tttctacaag ttttaatttt ttgttgattt ccgttgcatt ttgttctgtt tcacgcagtt
     1381 tatcggcaat tttatcataa gcggatttat tctcgttaat taatttttgt aaacgaattt
     1441 tttccaaagt aatgttattt ggtactgaac gtaaattaga aagagcattt gttaaattac
     1501 tttctctctc tttcaactca tctattcttt gtt
//
LOCUS       DTVV01000403            7000 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2195042, whole genome shotgun sequence.
ACCESSION   DTVV01000403 DTVV01000000
VERSION     DTVV01000403.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2195042"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="EYO86_04780"
     CDS             <1..501
                     /locus_tag="EYO86_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC42379.1"
                     /translation="KICSFFNKHARNTDTPILDVGAGTGLVGECLNKTGNKKIIGIDI
                     SPEMLEQAKLKGCYSSLVSANVTKKIPLKNNSVGAVVSAGTFTHGHVGPDAFDELLRI
                     TKPGGLFVLSINSKIFIKGGFQEKFSRIKNSISVPIFNEFKAHGNNKDENFSKIKIFA
                     SIFRKK"
     gene            complement(533..1474)
                     /locus_tag="EYO86_04785"
     CDS             complement(533..1474)
                     /locus_tag="EYO86_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIC42380.1"
                     /translation="MKKIIVYTHSDCLLKDNGPNHPEKKERVQIILKSIQNISSIDID
                     IKNAPLADVRNIYLVHPSTHINNIFSLIPNSGLTGIEKEPYADTLLCPKSKKAILRSC
                     GAGIAAADSLIRENEKRIFCAVRPPGHHAETVRANGFCFVNNVAVTARYLQSKYEINK
                     IAIIDFDVHHGNGTQEIFFKDETVAYGSIHEFPLFPGTGSEQEKGMGNIFNAPILAGT
                     EGKDFIKILENKILNNIDKFRPEIILISAGFDAHKRDPLAHINLESKDYYDLTKKIIE
                     IANIHCQGRIISFLEGGYDLLALSESIKEHLLGLQEN"
     gene            1628..2086
                     /locus_tag="EYO86_04790"
     CDS             1628..2086
                     /locus_tag="EYO86_04790"
                     /inference="COORDINATES: protein motif:HMM:PF04386.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42381.1"
                     /translation="MKIHYSQILKKNMLNVFRDVLKNIEVNGLQEGHHLYISFKTDNK
                     KVVIPNWLKEKHPKEITIVIQYEYWNFKVKNDSFNIGLSFNDINADISVPFDSVISFA
                     DPYANFGLKLINEEINKKLSDKKPKRKKSVIKKNKDKDNIIEFQKFKKLR"
     gene            complement(2078..2707)
                     /locus_tag="EYO86_04795"
     CDS             complement(2078..2707)
                     /locus_tag="EYO86_04795"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIC42382.1"
                     /translation="MNNLILLRHGQSQWNLENRFTGWEDVPLTEQGIAEAKVAGQLMK
                     KNKVHIDVIFSSVLQRATKTAELAIKEMNLEQFWSDGQLLMTKDQSLNERDYGDLVGF
                     NKEETANKYGKEQVRIWRRSYDVSPPGGESLENVVTRVKPYFINFIEPKINEGQDVLI
                     AAHGNSLRASMIQVGLYKAEEISKIEIPTGSPFIITYESGKVVNSKYLS"
     gene            2777..4162
                     /gene="fumC"
                     /locus_tag="EYO86_04800"
     CDS             2777..4162
                     /gene="fumC"
                     /locus_tag="EYO86_04800"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIC42383.1"
                     /translation="MKKKYRIEFDSLGSKKIDSTQLWGAQTQRSLENFKIGNEKIPKE
                     IIIAFGHQKKAAALANIKIGKLNSKIGKAIIDACNQIINLKLIDEFPLSIWQTGSGTQ
                     TNMNANEVISNYAIKKLKGEIGSKKPVHPNDHVNLSQSSNDTFPTVMHITCNELLQDK
                     LNISLRNLIKELKSKSQKFNRIIKIGRTHTQDAIPLTLGQEFSGYLEQIKNNQKRILQ
                     AQKELLYLAQGGTAVGTGMNAPKNFDKIFCKYLSILTKSKYLPAPNKFESIASHDPMV
                     NLSGSLNTLATSLLKISNDLRFLASGPRSGLGELKLPENEPGSSIMPGKVNPTQIEAL
                     SMVCTQVIGNHTTITIAGSQGHFELNAFKPVIIFNNLQSIHLLSDSIDSFIKYCLRGL
                     KPDVYRIKQHLDNSLMLVTALNEHIGYDNAAKIAKKAFKENLTLKEAAIKLGMIEANE
                     FDRIVQPKKMI"
     gene            complement(4170..5042)
                     /locus_tag="EYO86_04805"
     CDS             complement(4170..5042)
                     /locus_tag="EYO86_04805"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:PRK13186.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIC42384.1"
                     /translation="MIASSNTSKKQQTINSSISLSGVGLHSGKKVAINIEPAEINNGI
                     RFIRTDQKNSNIINALWSNVTSTNLCTTIANKEGASVSTIEHLMSALSGMHIDNVNIL
                     IDGPEVPIMDGSSLPFVELFESKGIVKQEIDRQIILIEKEVVVTKGDSFTKILPDKQF
                     SINFEIEFASHLVNKQACQLQLVNGNYKKDISFARTFGFEKDVDYLRANGFALGGSLE
                     NAVVVGDKKILNKEGLRFEDEFVRHKMLDSIGDLYLAGKPIQGYFYGKKSGHFLNIQL
                     LRKIFSDKSNFKII"
     gene            complement(5144..6778)
                     /gene="ftsZ"
                     /locus_tag="EYO86_04810"
     CDS             complement(5144..6778)
                     /gene="ftsZ"
                     /locus_tag="EYO86_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIC42385.1"
                     /translation="MTINISIPDVGPNLHPQITVLGVGGSGGNAVNNMMNSNLEGVDF
                     VIANTDAQALQISNCKKKIQLGIKSTRGLGAGMRPDVGRQAAEETLNEISEKLDGSHM
                     LFIAAGMGGGTGTGAAPVIAKLAREKGILTVGVVTKPFHFEGSQRMKIANKGLEELQQ
                     YVDTLLTIPNQNLFRIANEKTTFADAFKMADDVLYAGVRGVTDLMVQPGLINLDFSDV
                     KTVMSEMGRAMMGTGEASGEGRAVAAAEAAIANPLIDDVSLKGAKGLIINITGGNDIT
                     LYEVDEAANRIKQEVDEDANIIYGTTCDERLEGVVRVSLVATGIDLNSETSPKPLEAV
                     STLSIDNSVYLQKSSEFNQNTEVIKEDLDKELTLENTVKDEIVDSSDEKALPSENPSI
                     NYINNDVEEDTSGALFAEKANIEAAQSSYDEEQIEKSNELEQVDSNIYEENQVFSNSS
                     AVKEEVKSSVRKLSLFDTLEDKNLESEPIENSTSVKSEPVFQNEILEEDHEITNTKEN
                     ENEFSDDEGPESEDDFNQENEEELLDIPTFLRRQAN"
BASE COUNT         2371 a         1199 c          960 g         2470 t
ORIGIN      
        1 aaaatttgta gtttttttaa taaacatgca agaaatactg atactccaat tcttgatgtt
       61 ggtgctggaa ccggtttggt tggtgaatgt ttaaataaaa caggcaataa aaaaatcatt
      121 ggaatagata tatctccaga aatgttggaa caagcaaaat taaaaggatg ttattcttcc
      181 ctagtgagtg ctaatgttac aaaaaaaatt ccattaaaaa ataattctgt tggagcagtt
      241 gtaagtgcag gaacttttac tcatgggcat gtgggaccag atgcttttga tgaactctta
      301 cgtattacaa aaccgggagg attattcgtt ttatccatta attcaaaaat atttatcaag
      361 ggtggttttc aagaaaaatt ctcaagaatt aaaaacagta tttctgttcc tatttttaat
      421 gaatttaaag ctcatggtaa taataaagat gaaaacttta gcaaaataaa aatatttgca
      481 tctatttttc gtaaaaaatg aaaaatggaa attaatacaa caaactgtta atttaatttt
      541 cctgtaaacc caacaaatgt tctttaatac tttccgatag tgccaataaa tcgtaacctc
      601 cttccaaaaa agatataatt cttccttggc aatgtatatt tgcaatttct ataatttttt
      661 ttgttaaatc ataataatct tttgattcta aattaatatg cgccaaagga tctcttttat
      721 gcgcatcaaa accagcggat attaatatta tttcaggtct aaatttatct atattattca
      781 aaattttatt ttctaatatc tttataaaat cctttccttc tgttcctgct agaattggtg
      841 cattgaaaat gtttcccatt cctttttcct gttctgaacc tgtgcctgga aataatggga
      901 attcgtgtat agaaccataa gcaactgttt cgtctttaaa gaaaatttct tgcgtgccat
      961 ttccatgatg aacatcgaaa tcaataatcg caatcttatt tatttcatat ttgctttgta
     1021 aataccttgc agtaactgca acattattta caaaacaaaa tccatttgct ctaacagttt
     1081 ctgcgtgatg acctggtggc cgaacagcgc aaaatattct tttttcattt tccctaataa
     1141 ggctgtctgc ggcagcaata ccagctccac aagatcttaa tattgctttt ttacttttgg
     1201 gacataaaag tgtatcggca tatggctctt tttctattcc cgttaatcca gaatttggaa
     1261 ttaatgaaaa aatattgttt atatgtgttg atggatgtac cagataaata ttacgaacat
     1321 ctgctaatgg tgcattttta atatctatat caatactgct tatattttga atggatttaa
     1381 gaataatctg tacacgttct tttttttctg ggtgatttgg accattatct ttgagtaagc
     1441 agtctgagtg tgtgtaaaca attatttttt tcattactcc atcttacatt tcttttatta
     1501 gtttcaaaat tcgttatccg tatttgaata gctagtacac actttaactg aatactctta
     1561 cgaaaaactg tatatactat gtttaagtga tgtttcttct aattacagaa catagcaata
     1621 tttaaaaatg aaaatccact attctcaaat cttaaaaaaa aacatgctaa atgtttttcg
     1681 cgatgttcta aaaaatatag aagtaaatgg tttgcaagaa ggccatcatc tttatatatc
     1741 ctttaaaaca gataataaaa aagttgttat tccaaattgg ttaaaggaaa aacatccaaa
     1801 agaaatcacg atagtcattc aatatgaata ttggaatttc aaagtaaaaa atgactcatt
     1861 caatattggg ctatcattta atgacataaa tgcagacata tctgttccat ttgattctgt
     1921 aatctctttt gccgatccat atgcaaattt tggattaaaa cttataaatg aagaaattaa
     1981 taaaaaatta agtgacaaaa aaccaaaaag aaaaaaatct gttattaaaa aaaataaaga
     2041 caaagataat ataatcgaat ttcaaaagtt taaaaaatta agataaatat ttagaattca
     2101 caactttgcc agattcataa gttataataa atgggctgcc agttggaatc tctatttttg
     2161 aaatttcttc tgctttatac aagccaactt gtatcatcga tgctcgcagt gaattgccat
     2221 gggctgctat taacacatct tgaccctcat tgattttagg ttcgataaag ttgataaaat
     2281 atggcttaac tcttgtaaca acattttcca gactttctcc tcctggagga gaaacatcgt
     2341 acgaacggcg ccatatgcga acttgctctt taccgtattt attagcagtc tcttctttat
     2401 taaatccaac caaatctccg taatccctct catttaaact ttggtctttg gtcataagaa
     2461 gttgaccgtc actccaaaat tgttccaagt tcatttcttt aattgctaac tcagccgttt
     2521 tggtggctct ttgcaaaaca ctactgaaaa ttacatcaat atgcacttta ttttttttca
     2581 ttaattgacc agcgacttta gcctctgcaa tgccctgttc ggtcaatggt acatcttccc
     2641 atccagtaaa tcgattttca agattccatt gactttggcc atggcgtaaa aggataaggt
     2701 tattcataaa ataaatattt ttaattcaat gattgagttt atgttataca aaaatataat
     2761 taaattaata caaaaaatga aaaaaaaata cagaatagaa tttgatagtt tgggaagcaa
     2821 aaaaatagac tctacccaac tatggggagc acaaacacag cgatcacttg aaaatttcaa
     2881 aattggtaat gaaaaaattc caaaagaaat aatcattgca tttggtcatc agaaaaaagc
     2941 tgccgctctt gccaatataa aaattggcaa attaaattct aaaatcggaa aagcgatcat
     3001 tgatgcctgt aatcaaatta tcaatttaaa gcttattgat gaatttccac tttctatctg
     3061 gcaaacaggt tcaggaacgc aaaccaatat gaatgctaat gaagttatta gtaattatgc
     3121 tattaaaaag ttgaaagggg aaattggatc taaaaaacca gtccatccta atgatcatgt
     3181 taatctatcc caatcttcta atgatacttt tccaacagta atgcacataa cttgcaatga
     3241 attgttacaa gataaattaa acatatcatt acgtaatttg attaaagaat taaaatctaa
     3301 gtcacaaaaa ttcaacagaa ttataaaaat tggtcgaaca catactcaag acgctatacc
     3361 tttaacatta ggacaggaat tttcaggata tttagaacaa ataaaaaata atcaaaaaag
     3421 aatattacaa gctcaaaaag aactgcttta tttagcccaa gggggaacag ctgttggaac
     3481 aggtatgaat gctcctaaaa attttgataa aattttttgt aaatacttat caatattaac
     3541 taaaagcaaa tatttacctg cccctaataa atttgaatcc attgcatcgc acgatcccat
     3601 ggtaaaccta tctggaagtc ttaatactct tgctacttct cttttaaaaa tatcaaatga
     3661 tttacgtttt ttagcatcag gtccacgttc aggtttagga gagttaaagt tgcctgaaaa
     3721 cgaacctgga tcttctataa tgcctggtaa agtaaatcca acacaaattg aggcgttatc
     3781 aatggtgtgc acccaagtaa ttggaaatca cacaacaatt actattgctg gatcgcaagg
     3841 tcactttgaa ctaaatgctt ttaaaccagt gattattttt aataatttac aatcaataca
     3901 tttactttct gattcaattg acagttttat taaatactgt cttcgaggtt taaagccaga
     3961 tgtttatcga ataaaacaac atttagataa ttctttaatg cttgtcactg ctttgaatga
     4021 acatataggg tatgacaatg ctgcaaaaat tgcaaaaaaa gctttcaaag aaaacttaac
     4081 acttaaagaa gcagctataa aacttgggat gatagaagca aatgagtttg ataggatagt
     4141 tcaaccaaaa aaaatgattt aggtttagat taaattatct tgaaattaga tttatctgaa
     4201 aatattttac gcaaaagttg aatgtttaaa aaatggcctg atttttttcc atagaaataa
     4261 ccttgaatag gtttgccagc taagtataaa tctcctattg aatccagcat cttatgtcga
     4321 acaaactcat cttcaaaacg taaaccttct ttatttaaaa tttttttgtc cccaactaca
     4381 acggcatttt caagtgaacc tccgagagca aatccgttag ctctcaaata atctacatct
     4441 ttttcaaatc caaaagttct cgcaaaagaa atatctttct tatagttgcc attaactaat
     4501 tgaagttggc atgcctgttt attaactaaa tgacttgcaa attctatttc aaaattaatt
     4561 gaaaattgtt tgtcaggaag aatttttgta aaactatcac ctttggtaac tacaacttct
     4621 ttttctatca aaattatttg gcgatcaatc tcttgtttta ctataccttt actttcaaat
     4681 aattctacaa aaggtaatga actaccatcc atgatgggaa cttcaggtcc atcaatcagt
     4741 atatttacat tatcaatatg catacctgat agagcactca ttaaatgctc tatagtagat
     4801 actgaagctc cttctttatt tgcaatagtt gtacataggt tcgttgaagt aacatttgac
     4861 cataatgcat taatgatatt gctatttttt tgatcagttc ttataaatct tattccattg
     4921 ttaatttctg ctggctcaat atttatagct actttctttc ctgaatgtaa gcctacacct
     4981 gataaagata tgctcgaatt tattgtttgc tgttttttag atgtatttga gcttgcaatc
     5041 ataaatataa aaaaagtgct gtcctgcagc acttttctta aatatatata tttttatagt
     5101 ttcacaatta actgaatatt actgattatt tcaataaatt tagctaattt gcttgtcttc
     5161 taaggaaagt tggaatgtct aacaattcct cttcattttc ctgattaaaa tcatcttcag
     5221 attcaggacc ttcatcatcc gaaaattcat tttcattttc tttagtatta gttatttcat
     5281 gatcctcttc aagtatttca ttttgaaaaa caggttctga ttttactgaa gttgagtttt
     5341 ctatgggctc actctctaga tttttgtctt caagagtatc aaataaacta agtttgcgaa
     5401 cactagattt aacttcctcc tttacagctg atgagtttga aaacacttga ttttcttcgt
     5461 atatattaga atcaacttgt tctaattcat tagatttttc tatttgctct tcatcatatg
     5521 aggactgagc agcttctata tttgcttttt ctgcaaacaa agctcctgaa gtatcttctt
     5581 caacatcatt atttatataa ttaatacttg gattttctga tggtaaagct ttttcatcac
     5641 ttgaatcaac aatttcatct ttcacagtat tttctaatgt taattcttta tctaaatctt
     5701 ctttaataac ttccgtattc tgattaaatt cagatgattt ttgtaaataa acggaattat
     5761 caatagataa agtactaact gcttctagag gtttagggga ggtttcagaa tttagatcta
     5821 taccggttgc cactaaactt accctaacaa caccttctag tctttcatcg catgtagtcc
     5881 cataaataat attagcatct tcatcaactt cttgcttaat tctattagct gcttcatcta
     5941 cttcatataa agtaatatcg ttacctccag taatattgat aattaaacct tttgcgcctt
     6001 ttaaagatac atcatcaatt aaaggattag ctattgcagc ttcagcagca gcaacagcac
     6061 gaccttcacc tgaagcttct cctgttccca tcattgccct gcccatttct gacataactg
     6121 ttttcacatc tgaaaaatca agattaatca aacctggttg aaccatcaaa tctgtaacac
     6181 ctctaacacc ggcatataaa acatcatctg ccattttaaa tgcatctgca aatgttgttt
     6241 tctcattagc aatacgaaat aaattttgat ttggtatagt caagagagta tcaacatact
     6301 gttgtaactc ttcaagaccc ttgttagcga tcttcattct ttgagagcct tcaaaatgaa
     6361 aaggttttgt aacaactcca acagttaata ttcctttttc tcttgcaagt ttagctataa
     6421 ctggtgcggc acccgttcct gtaccacctc ccatacctgc agcaataaat aacatatgac
     6481 ttccatctaa tttttcactt atttcattaa gagtctcttc tgctgcttgc cttccaacat
     6541 cgggtcgcat accagctcca agtcctctag tgcttttaat tcctaattga atttttttct
     6601 tgcaatttga aatttgaaga gcttgtgcat ctgtatttgc tatcacaaaa tcaactccct
     6661 caagatttga attcatcata ttgttaacag cgttccctcc tgatccgcca acacctaaaa
     6721 ctgtaatttg aggatgtaga ttaggaccta catctggtat agaaatattt atagtcatgt
     6781 atttactccg ccgttttttt atatttttta acgatttacc cccgcaaccg tcaataaata
     6841 taactatata ttgtatgtta tattatatat attgatctag ccaagaagaa aaacggccaa
     6901 ttaaggtatt tttttttatt ttttgggctt ttttcataaa atttacttca tatttatcaa
     6961 cttcatgcaa aattaaacca atagtttgag aaaattgagg
//
LOCUS       DTVV01000404            1045 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2201635, whole genome shotgun sequence.
ACCESSION   DTVV01000404 DTVV01000000
VERSION     DTVV01000404.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2201635"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="EYO86_04815"
     CDS             <1..921
                     /locus_tag="EYO86_04815"
                     /inference="COORDINATES: protein motif:HMM:PF03989.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIC42386.1"
                     /translation="KGKVYQMKVFKLPEFAPNARGKPMVGIFPLTKNENITAVLPLPN
                     DKTSWENLNIMFSTSKGTVRRNALSDFIKIQSSGKIAMKLSDDDKLIGVHTCIEKEDD
                     VFLSTKEGKCLRFPISNVRKFSGRNSVGVRGIRLSKVDMVISMYILKHAKFTIDERND
                     YLKIANAIRRKELRRSTPFNKLSKGTQLSENKFVEMENIEEFILSIADNGFGKRSSAY
                     EYRITSRGGKGIANMSVNKKIGKVVSSFPVNEKDDIMLIAGTGKLIRSPVNKIRISGR
                     TTQGVTLFKVESSEKVLSAASIKDLNISSE"
     gene            938..>1045
                     /gene="coaD"
                     /locus_tag="EYO86_04820"
     CDS             938..>1045
                     /gene="coaD"
                     /locus_tag="EYO86_04820"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371809.1"
                     /note="Catalyzes the conversion of ATP and pantetheine
                     4'-phosphate to diphosphate and 3'-dephospho-coA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIC42387.1"
                     /translation="MKVAIYPGSFDPITNGHLDIITRSSKIVDRLIIAVS"
BASE COUNT          417 a          111 c          183 g          334 t
ORIGIN      
        1 aaaggaaaag tttatcaaat gaaagttttt aaattacctg aatttgcacc aaatgctaga
       61 ggtaaaccaa tggttggaat ttttccttta acaaaaaatg aaaatataac tgcagtttta
      121 cctttaccta atgacaaaac ttcttgggaa aatttaaata ttatgttttc aacatctaaa
      181 ggtacagtta gaagaaatgc gctatcagat tttattaaaa tacaatcaag tggaaaaata
      241 gcaatgaaac tgtccgatga tgataagtta atcggtgttc atacttgtat cgaaaaagaa
      301 gacgatgttt ttttatcaac taaagaagga aaatgtttaa gatttcctat atctaatgta
      361 agaaaatttt ccggtagaaa ttctgtagga gttagaggta ttagattatc aaaagttgat
      421 atggtcattt caatgtacat tttaaaacat gcaaaattta ctatagatga aaggaatgat
      481 tatttaaaaa ttgctaatgc aataagaaga aaagagttaa gaagaagtac gccgtttaat
      541 aaactttcaa aaggaacaca attaagtgaa aataaatttg ttgaaatgga aaatattgaa
      601 gaatttattt tgtctattgc agataatggt tttggaaaaa gaagttcggc atatgaatat
      661 agaattacta gtaggggtgg aaaaggaatt gctaatatgt cagttaataa aaaaattggt
      721 aaagtagttt cttcatttcc agttaatgaa aaagacgata taatgcttat agccggaaca
      781 ggaaagttaa ttaggtcacc tgtaaataaa atcaggattt ctggacgtac aacacagggt
      841 gttactttat ttaaagttga atcaagtgaa aaagtattat ctgctgcttc aattaaagac
      901 ttgaatatta gttctgaata ataaaatata ataaattatg aaagttgcaa tatatccagg
      961 aagttttgac cctatcacta atgggcattt agatataatt acaagatcgt ctaagattgt
     1021 agataggtta attatcgcag tatct
//
LOCUS       DTVV01000405            1021 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2216994, whole genome shotgun sequence.
ACCESSION   DTVV01000405 DTVV01000000
VERSION     DTVV01000405.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2216994"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1021)
                     /gene="sucA"
                     /locus_tag="EYO86_04825"
     CDS             complement(<1..>1021)
                     /gene="sucA"
                     /locus_tag="EYO86_04825"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038192.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIC42388.1"
                     /translation="DKTNDKVFGLLIHGDAAMAGQGIVAETFAMSQLVGFRTGGTIHF
                     VINNQIGFTTMPQYGRSAPYCTEIAKMVQAPIFHVNGDDPEAVVHVCRIATEFRNKFK
                     VDVVVDMFCYRRSGHNEADEPSFTQPLMYQAIKKHQSTVKIYEKQLLEEKILTNEELK
                     KIQTNFKKFLDKEFESAKSYKPNKADWLEGDWTGLSTASFDARKGATAVKENELQEIA
                     KAIHQIPTDFNIHKKIKKVYEERQSSIHEGKAIDWSTAEALAFATLLKEGYGVRLSGQ
                     DTGRGTFSQRHAVLYDQTNEKRFVPLRHFIKQQGYFEIIDSFLSEYGVLGFEYGYSQS
                     DPKTLV"
BASE COUNT          299 a          194 c          154 g          374 t
ORIGIN      
        1 aacaagtgtt ttagggtcag attgtgaata accatattca aatcctagaa ctccatactc
       61 agataaaaaa ctatcaatta tttcaaagta cccttgttgt tttatgaagt gtcttagggg
      121 aacaaatctt ttttcattag tttgatcgta tagaacagca tgacgttggc taaatgtccc
      181 tcgcccagta tcttgcccag ataatctaac tccatagcct tcttttaata aagttgcaaa
      241 agctaaagct tcagctgtag accaatcaat ggctttgcct tcatgaatag agctttgtct
      301 ttcctcatat acttttttta tttttttatg aatattaaaa tccgtaggta tttgatgaat
      361 tgcttttgca atttcttgta attcattttc tttaaccgca gtcgctcctt ttctagcatc
      421 aaaagatgct gtagataatc ctgtccaatc tccctcaagc caatctgcct tgttaggttt
      481 atatgatttt gcggactcaa attccttatc aagaaatttt ttaaaattag tttgtatttt
      541 ttttaactct tcattagtca aaattttttc ttctaataat tgtttttcat atatttttac
      601 tgttgattgg tgctttttta tagcttgata cattaaaggt tgggtaaaag atggctcatc
      661 tgcttcattg tgacctgaac gacgataaca gaacatatct acgactacat ctactttaaa
      721 tttatttctg aactctgttg ctatgcgaca aacatgaact acagcttctg gatcatctcc
      781 attaacatgg aaaataggcg cctgaaccat tttggctatc tctgtacaat aaggggctga
      841 acgtccatat tgtggcattg ttgtaaaccc aatttgatta ttaattacaa aatgaatagt
      901 tcctccagtt cgaaaaccta ctagttgcga catagcaaat gtttcagcaa ctattccttg
      961 tccagccatt gcggcatcac catgaatgag aagaccaaaa accttatcat tagttttatc
     1021 t
//
LOCUS       DTVV01000406            1571 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2217840, whole genome shotgun sequence.
ACCESSION   DTVV01000406 DTVV01000000
VERSION     DTVV01000406.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2217840"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            146..673
                     /locus_tag="EYO86_04830"
     CDS             146..673
                     /locus_tag="EYO86_04830"
                     /inference="COORDINATES: protein motif:HMM:PF08491.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42389.1"
                     /translation="MPYINNQSGIVTTATKDLASILMRMDKKKFNKEMESRFNNFFGK
                     MKLLGKRYSYPMVTTYTKKFISHRFAVIGDAAVGMHPVTAHGFNLGLKGLDILIDEIK
                     AALKNKSDFGLTDTLKNYQYKLHRVAAPVYLATNSIINLYTSNILPAKLTRQFILRFV
                     NTIKPVKQTFLNILK"
     gene            complement(1064..1546)
                     /locus_tag="EYO86_04835"
     CDS             complement(1064..1546)
                     /locus_tag="EYO86_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIC42390.1"
                     /translation="MKLKEGDALPDAKVFIFDESPKETSIKQIIGEERVIIFGLPGAF
                     TPTCSTKHLPGFIGATDQLKAKDIKKVICISVNDPFVMDAWGKIHKVENKILMVGDSN
                     AVFTKNIGAELDLNKKGLGIRSSRYTILVEKGSIVKISEEEVAGKCESTAAENFIKEI
                     "
BASE COUNT          518 a          269 c          213 g          571 t
ORIGIN      
        1 tcacgtttcg gtcccgtaga aataatagat acctttgctc ctaccatctc agaaagtccc
       61 tcaagataag ctacaaaggt ttcggttata gaatacgtgc cttccagcct gttaattttt
      121 tcgttataat cagacacaag caacattgcc atacatcaat aatcaatcag gtatcgttac
      181 aactgctacc aaagatttgg catctatttt gatgagaatg gataaaaaaa aatttaataa
      241 agaaatggaa agcaggttta ataatttttt tgggaaaatg aaattattag gaaagcgtta
      301 ctcttatcct atggtaacca cctacacaaa aaaatttata tctcataggt ttgctgtgat
      361 tggtgatgct gcagtaggta tgcatccagt aactgcacac ggatttaatt tgggtttaaa
      421 aggattagac attttaattg atgaaattaa agctgcccta aaaaataaaa gtgattttgg
      481 attaacggat actcttaaga attatcaata taaattacat cgtgttgccg ctccggtata
      541 ccttgccaca aatagtataa tcaatttata cacatctaat attttgcctg ccaaacttac
      601 aagacaattt atattaagat ttgttaatac cataaagcca gttaaacaaa cttttctaaa
      661 tatattaaaa taaactaggc cgcattagtg tcaacgttga ctacattact ctttttagga
      721 acaaagaaaa aaattcaaaa gcttttaaaa aaatgatgca actaatataa attaattaat
      781 gttttaaatg attaatcttg tctttaagtt gcagttttag tttttttaat tctcttaaat
      841 tcttccaaga atcggatgac ctatcattta atcttttctt tgtagctttc tttgtactag
      901 ttgctaattt tttatgctct ctcttaagat ctttaagtgc cgtcataaca aatcgacagt
      961 aaagcacata tgtgacttaa ttatgagttg ttaatcactt tattgttgta ttaaataaat
     1021 agtagcaatg tactaaatgc ttatatattc ataaatagag ttattaaatt tcttttataa
     1081 aattttctgc tgcagtactt tcacattttc ccgcaacttc ttcttcagaa attttaacga
     1141 tacttccttt ttctaccaat atcgtgtacc tgcttgatcg aattcctaat ccttttttat
     1201 ttaaatcaag ttctgcgcca atattttttg taaaaacggc gttagaatct cctaccatta
     1261 atattttatt ctcaacctta tgtattttcc cccacgcatc cattacgaaa ggatcattta
     1321 ctgaaataca aataactttt ttgatatctt ttgctttaag ttgatcggtt gcaccaataa
     1381 atcctggtaa gtgttttgtt gaacatgttg gtgtaaaggc cccaggaaga ccaaaaatta
     1441 taactctttc ttcaccaatg atttgcttaa ttgacgtctc tttggggctt tcatcaaaaa
     1501 taaatacttt tgcatcaggt agtgcatctc cttcttttag cttcatatat ttaactccct
     1561 attaacaata a
//
LOCUS       DTVV01000407            1554 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2218119, whole genome shotgun sequence.
ACCESSION   DTVV01000407 DTVV01000000
VERSION     DTVV01000407.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2218119"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1..69
                     /locus_tag="EYO86_04840"
     tRNA            1..69
                     /locus_tag="EYO86_04840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:27..29,aa:Arg,seq:tct)
     gene            117..458
                     /locus_tag="EYO86_04845"
     CDS             117..458
                     /locus_tag="EYO86_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIC42391.1"
                     /translation="MLYKVSCHCGSVQLEVETDLSMIKQCNCSICKRKNAKMSILPKE
                     AIKSIKGEDNLSVYQFGTKVAKHYFCKICGIYTHHQRKSDPNGIGVNIGCIDELDSFS
                     IKSDVLDMRNK"
     gene            504..1484
                     /gene="iolC"
                     /locus_tag="EYO86_04850"
     CDS             504..1484
                     /gene="iolC"
                     /locus_tag="EYO86_04850"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HIC42392.1"
                     /translation="MDLENTLKKISENNFLIIGRAGIDIYPDPPGTKTELAKKFVTHL
                     GGSSANIAVALTKLGGRCKLLTSVSDDALGRLALNQLNDYNVDTSLIRLEEGEARISF
                     AVVESRIEDHQSIIYRHGAADLLMKKEYIERVNFNDFSCLLFTGTCLASEPSRGAIFL
                     SLEKAKTANIPVVLDIDYRPYTWESSQEASKVYLKASSLCDILIGNDDEFGVLAMKFE
                     DSFQQAKELSKNISLVIYKKGEKGSVALYNNKEVTKGIFPVKALKPTGAGDAFMGAFI
                     GSLLKVKPVEESIEIGSAAAAIVVTKVGCAPAMPMQNEIEEFMKINKITK"
BASE COUNT          549 a          210 c          283 g          512 t
ORIGIN      
        1 agctcagttg gatagagcat cggttttcta aaccgagggt cgcaggttcg agtcctgcag
       61 agcgcgccat aaatattatg tctttatact ataattttat ctgttaatga tattttatgc
      121 tctataaagt ttcttgtcat tgcggttcag tacagttgga agttgaaact gatctttcaa
      181 tgattaaaca atgtaattgc tcaatttgta aaagaaaaaa tgctaaaatg agtatcttac
      241 caaaagaagc tataaaatcc atcaaaggtg aagataactt atctgtttat caatttggta
      301 ctaaagtagc aaagcattac ttttgtaaaa tttgtgggat ttatactcat catcaaagaa
      361 aatctgatcc caacggtatc ggtgtaaaca ttggttgcat tgatgaatta gactcttttt
      421 ctattaagtc tgatgtttta gatatgagga ataaataaat tttaatcgtt tttttataaa
      481 tatatatatt tattttttaa aatatggatc ttgagaatac tttaaaaaaa atttctgaaa
      541 ataatttttt gattataggt agggcaggta tagacattta tcctgatcca cctggtacta
      601 aaactgaatt agcaaaaaaa tttgtaactc atttgggagg atcttcagcc aatatagcag
      661 tcgcattaac taaattgggt ggtcgatgca aattattgac atctgtatct gatgatgctc
      721 ttggtagatt agcgttaaat caattaaatg attataatgt tgatacttca ttgataagat
      781 tggaagaagg tgaagcacga atttcatttg ctgttgtaga atcaagaatt gaagatcatc
      841 aatcaatcat ttatcgtcat ggggctgcag atttacttat gaaaaaagaa tatatagaaa
      901 gagttaattt taatgatttt tcctgtttac ttttcactgg aacatgtcta gcctccgaac
      961 catctagggg agcaatattt ttaagtttgg aaaaggcaaa aactgctaat attccagtag
     1021 ttttagatat tgattataga ccctatacat gggaatcctc tcaagaagct tctaaagtat
     1081 atttaaaggc atctagtttg tgtgatattc ttattggaaa cgatgatgag tttggtgttt
     1141 tagcaatgaa atttgaagat agttttcaac aagctaaaga acttagtaaa aatatttcct
     1201 tagtaatata caaaaaagga gaaaaagggt ctgtagctct ttacaacaac aaggaagtca
     1261 caaaaggtat ttttcctgta aaagcactaa aaccaacagg ggctggagat gctttcatgg
     1321 gcgcatttat cggatcattg ttaaaagtaa aacctgttga agaatcaatt gaaataggct
     1381 ctgcagcagc agctatagtt gttacaaaag taggttgtgc gcctgcaatg ccaatgcaaa
     1441 atgaaattga agagtttatg aaaataaata aaataactaa ataaggagaa aatgtatgca
     1501 tatccccccc aatgataaca aaaatattcc cattgtagat attgatgact caag
//
LOCUS       DTVV01000408            1106 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2221024, whole genome shotgun sequence.
ACCESSION   DTVV01000408 DTVV01000000
VERSION     DTVV01000408.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2221024"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="EYO86_04855"
     CDS             complement(<1..908)
                     /locus_tag="EYO86_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIC42393.1"
                     /translation="MTEWPILTVTIFLPILGASIIFFIKEEETSLSNIKWAALLTSIG
                     TFILSCLIWLQFDNSIAEYQLSEKHQWFTDFNFNYHIGVDGISLFMILLSTFLTPFCI
                     LASWISINKRIKEYMMAFLFLETVLLGMFSSIDILLFYIFFESVLIPMFLIIGVWGGE
                     RRIYATFKFFLYTLLGSVLMLVAIIFIYQLTNSMSISELQGNFFSKNIQIYLWLAFFA
                     SFAVKIPMWPFHTWLPDAHVEAPTAGSVILAGVLLKMGGYGFIRFSLGMLPEASEFFL
                     PLIMIMSIIAIIYTSLVALAQTDIKKL"
BASE COUNT          462 a          182 c          160 g          302 t
ORIGIN      
        1 agttttttga tatcagtttg agctaaagca acaagggatg tgtaaattat tgcaataata
       61 ctcataatca taatcaaagg taagaaaaac tcagaagctt ctggaagcat cccaagagaa
      121 aaacgaataa aaccataccc acccattttt aataaaacgc ctgctaagat aactgaccct
      181 gctgttgggg cttcaacgtg ggcatcaggc aaccatgtat gaaagggcca cattggaatt
      241 ttaactgcaa aagaggcaaa aaaagctaac cataaataaa tttgaatatt tttggagaaa
      301 aaatttccct gaagttcact gatactcata gaattggtta actgataaat aaagataatt
      361 gcaactaaca tgagaacaga acctaacaag gtatataaaa aaaatttaaa cgtggcgtat
      421 atacgtcttt ctcctcccca cactccaata attaaaaaca taggaataag tacggattca
      481 aaaaaaatat aaaacaataa tatatcaatt gaagaaaaca tacctaacag aacggtttct
      541 aaaaaaagaa atgccatcat gtattcttta attctcttat taatacttat ccaactagct
      601 aaaatacaaa aaggggttaa aaaagtacta agcaaaatca taaataatga aattccatcg
      661 acaccaatat gatagttaaa attaaaatca gtaaaccact gatgtttttc acttaattga
      721 tattcagcga tggaattgtc gaattgtaac caaatcaaac aactaagaat aaacgtacca
      781 atggaagtca aaagagcagc ccatttaata ttgcttaaag aagtttcttc ctccttaata
      841 aaaaaaatga ttgaggctcc caatataggt aaaaaaattg ttacggttaa tatgggccat
      901 tcagtcatat tagtaataaa caaaccatgt taaaattata actaagccac ccaacatgac
      961 aaaggcataa tggtagagat agcctgactg aaacaagctt aatctcttag aaaaataatt
     1021 gattaatttt gagatgccgt ttggtccata ttcatctatt atgtagatat ctcctttttt
     1081 ccaaaaaata gatgcgatat aaaaaa
//
LOCUS       DTVV01000409            1265 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2224565, whole genome shotgun sequence.
ACCESSION   DTVV01000409 DTVV01000000
VERSION     DTVV01000409.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2224565"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="EYO86_04860"
     CDS             complement(<1..862)
                     /locus_tag="EYO86_04860"
                     /inference="COORDINATES: protein motif:HMM:PF02219.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42394.1"
                     /translation="MNRNQKEFNIKKLLNNYSIETTPNVYEKYGKFSDLISTDNAVYV
                     TYLPDENSSRVINTAKKLTNEGFDVIPHLPARTIKDNNELEKYIGDLSEKAGCNKILV
                     IGGGSNQKGNITSSLDILKTDLLSKYQFKEVGLAGHPDGNPDISEEELDKAIIEKNEF
                     VKNVDYKAYLATQFFFEVSSLEIWEQKLNLHNNKLEIHAGIPGPATLKTLIAYAKSCG
                     IGNSIHFLSKQARNITKIMKTRTPDKLIADLADYKEERPNSKLAKLHFYAFGGIKTTS
                     EWLYDVINSNL"
     gene            903..>1265
                     /locus_tag="EYO86_04865"
     CDS             903..>1265
                     /locus_tag="EYO86_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42395.1"
                     /translation="MKNLKFTIITFYQFKKNRNLEKLQNLLKDFCSFHKIKGTILLAD
                     EGINGTVAGLIKPIKLLELEIQKLGFTRLELKRSFYELMPFNRLKIKIKNEIVTFDGS
                     QLDVENHSGKHVVSNEWNT"
BASE COUNT          428 a          171 c          175 g          491 t
ORIGIN      
        1 ctaaattaga attaataacg tcatataacc attcagaggt tgttttgata cctccaaagg
       61 catagaaatg taattttgct aattttgagt ttgggcgctc ttctttataa tcagcaagat
      121 cagcaatcaa cttatctgga gttctagtct tcattatttt tgtaatattt ctggcttgtt
      181 tagataaaaa atgtattgaa tttccgatac cacatgattt ggcatatgca attaaagttt
      241 taagagttgc tggacctggt atgccagcat gaatttctaa tttattgttg tgtaaattta
      301 atttttgctc ccaaatctct aaagaagaaa cttcaaagaa aaattgtgtt gctaaatatg
      361 ctttataatc tacattttta acaaattcat ttttttctat gatagctttg tctaactctt
      421 cttcagaaat gtcagggtta ccatcgggat gccctgctaa gcctacttct ttaaattggt
      481 attttgataa tagatcagtc ttaagaatat ctagacttga tgtaatatta cctttttgat
      541 ttgacccacc accaataacc aaaattttat tacaacctgc tttttcagat aaatcaccta
      601 tgtatttttc aagctcatta ttatctttta ttgtccttgc aggaagatgt ggaataacat
      661 caaatccttc attagtaagt ttttttgctg tattaattac acgtgatgaa ttttcatcag
      721 gtaagtaagt aacataaaca gcattatcag tggaaattaa atccgaaaat ttaccatatt
      781 tttcataaac atttggcgtt gtttctattg aataattatt taaaagcttt ttaatattaa
      841 attctttttg atttctattc atatatattt tattgatttt tatattttta aattggattt
      901 aaatgaaaaa tttaaaattt actattatta ctttttatca atttaagaaa aatcgtaatc
      961 ttgaaaaatt acaaaattta ctaaaggatt tttgctcatt tcataaaatt aaaggcacaa
     1021 ttttacttgc agatgaagga attaatggaa cggttgctgg gttaataaag cccattaaac
     1081 tgttagaatt agagatacag aaactaggat tcacaagatt ggaattaaaa cgttcttttt
     1141 atgaactcat gccttttaat cgtttaaaaa taaaaattaa aaatgaaatt gttacttttg
     1201 atggatcaca attagatgtt gaaaatcatt ctggcaagca tgttgtttct aatgaatgga
     1261 ataca
//
LOCUS       DTVV01000410            1832 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2227725, whole genome shotgun sequence.
ACCESSION   DTVV01000410 DTVV01000000
VERSION     DTVV01000410.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2227725"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="EYO86_04870"
     CDS             complement(<1..182)
                     /locus_tag="EYO86_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIC42396.1"
                     /translation="MKFIKKTILSLVLLAGISFSATSANAACKAHLGDFDWDSANIHT
                     AIASFIIEKGYGCDVEV"
     gene            319..1209
                     /locus_tag="EYO86_04875"
     CDS             319..1209
                     /locus_tag="EYO86_04875"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC42397.1"
                     /translation="MLIFNKFPNLTVIVSCFFWGSYWIPLRYIDSDSNGSLWPIFFSF
                     LLLSLLLLKPLVKTFYVIFYERNYFFFLGCLFAATGITFYSESLLRGEIAKVVVLFYL
                     CPIWGTIFAKIFLGNKLTLKRLLSIILGIIGLEIMVGIEKGIIFPTSVVEWIALSAGL
                     SWALGMTLFHLAKSTSGFEKTALTALIIPFVFLALCLVPDGRTLEMPNQLISVNVIYL
                     WMILFAVVWLLPSILLTYFSVEVLDPGRINILLAFEVAVGFISASLLTNEIIGLRECL
                     GALFVVSACFVDIFKSKIKY"
     gene            complement(1199..>1832)
                     /locus_tag="EYO86_04880"
     CDS             complement(1199..>1832)
                     /locus_tag="EYO86_04880"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC42398.1"
                     /translation="AVGCVVFYFFGLKFLLLSENIAIVHSAPIFAALLAVPILGERLG
                     IHRISAIVIGFIGVIIIVKPGTDVFKLVSILPLISALFMASVYLSTRSLMNTDSSIAI
                     IFYYSLALLITSIVFLPYDFTMPNIVQLIPLMSLGVVGSLGHYFMSQAAKNAEVVVIS
                     PFEYSSFIFVGIMGYIFYNEVPSISIIIGGILIIAGGVYIAYREQKISNI"
BASE COUNT          628 a          284 c          292 g          628 t
ORIGIN      
        1 acttccacat cgcaaccata acctttttca attatgaaag aagcaatggc agtgtgaata
       61 tttgcactat cccagtcaaa atcacccaaa tgagctttac aagctgcatt ggcactagta
      121 gcactaaatg aaatgccagc taataacact aatgacagga ttgttttttt tatgaatttc
      181 ataaatttcc tcctaaaaaa ttaattaatt aatctatggg cacttaaaca atttacgaga
      241 aaaaaaacaa cagtaaaaat ggagtttata aataaaaaat gctaaaaatt agccaaattt
      301 caataaaatt ttgtttatat gttaatcttc aataaatttc cgaatttaac ggtaattgtt
      361 tcctgttttt tttggggaag ttactggatt ccccttcgat atattgattc agatagtaat
      421 ggctcactat ggcctatttt ttttagtttt cttttattat ccttattact gttaaaacca
      481 ttggttaaaa ctttttatgt tatattttac gaacgtaatt acttcttttt cctgggttgt
      541 ttatttgccg caactggaat tactttttat tcagaatctt tgttgagagg ggaaatagcg
      601 aaggttgtag ttttatttta tttgtgcccg atatggggaa caatttttgc aaaaatattt
      661 ctgggcaaca aactaacatt aaaaagatta ttatccataa ttcttggcat tattggatta
      721 gaaataatgg ttggaattga aaaaggcatt atctttccaa cctcagttgt tgaatggatt
      781 gctttaagtg caggtctgtc ctgggccctg ggtatgactt tgtttcactt ggcaaaatca
      841 acaagtggtt ttgaaaaaac agcattaacc gcattgatta ttccttttgt ttttcttgct
      901 ttgtgtcttg ttcctgatgg aagaacttta gaaatgccaa accaacttat atcagtaaat
      961 gtaatatatt tatggatgat attgtttgct gttgtttggt tgcttccgtc aattttatta
     1021 acctacttta gtgtagaagt tttagaccca ggcagaatta atatactgtt agcttttgaa
     1081 gttgcagtcg gtttcatttc agcaagtttg ttaacaaatg aaattattgg cctacgtgag
     1141 tgtttgggag ccttgtttgt tgtgtcagct tgttttgtag atatttttaa atctaaaatt
     1201 aaatattaga aattttttgc tctcgatacg ctatatacac acctccagca ataattaata
     1261 ttcctccaat tattattgat atacttggta cctcattata aaatatataa cccataatgc
     1321 cgacaaaaat gaatgatgag tattcgaatg gagaaataac aacaacttca gcatttttag
     1381 ctgcttgtga cataaaataa tgacccaatg atcccacaac ccccaaactc attagtggaa
     1441 ttaattgaac aatgttaggc attgtaaaat cataaggtaa aaaaacaata gatgtaatga
     1501 gtaatgctaa agaataataa aatataatgg caatactaga atctgtattc attaatgaac
     1561 gtgtactcaa gtatacactg gccatgaata aagcagaaat taatggaagg attgaaacca
     1621 atttaaaaac atctgtccca ggtttaacaa taataatcac gcctataaaa cctatcacaa
     1681 ttgcgctaat tcgatgaatg ccaagtcgtt ctcctagtat tggaacagca agtaaagctg
     1741 caaaaattgg agcagaatga acaatggcta tattttccga aagaagtaaa aattttaaac
     1801 caaaaaaata aaaaacaacg caacctactg ct
//
LOCUS       DTVV01000411            1102 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2228513, whole genome shotgun sequence.
ACCESSION   DTVV01000411 DTVV01000000
VERSION     DTVV01000411.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2228513"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /gene="hslV"
                     /locus_tag="EYO86_04885"
     CDS             <1..454
                     /gene="hslV"
                     /locus_tag="EYO86_04885"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIC42399.1"
                     /translation="SMGNTIIKSTATKVRKIEKRNVIAGFAGSTADALTLFERLEAKL
                     EKHAGNLTRAAVELAKDWRTDKYLRRLEALMAIGDKEKSFIISGTGDVLEPEDNVIGI
                     GSGGNYALAAAKILMESDLSAEDVARKSIKVASDICVFTNNNVKIEKI"
     gene            520..>1102
                     /locus_tag="EYO86_04890"
     CDS             520..>1102
                     /locus_tag="EYO86_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIC42400.1"
                     /translation="MSSLSPREIVSELDRYIVGQNKAKKAVAIALRNRWRRQALEGDM
                     KDEVLPKNILMIGPTGVGKTEIARRLSKLAEAPFVKVEATRFTEVGYVGRDVEQIVRD
                     LLEIAIAMEKVKKRKEVYANAQKFAEERVLDAFVGNKASVATRESFRKRLRSGDLDDN
                     EIEIAINESNSMPSFEIPGMPGANVGMINLSDML"
BASE COUNT          453 a          128 c          230 g          291 t
ORIGIN      
        1 aagtatgggc aacactataa ttaaaagtac agcgacaaaa gttagaaaga ttgagaaaag
       61 aaatgtgatt gctggatttg caggttcaac tgcagatgca ctaacattat ttgaacgtct
      121 tgaagcaaaa ttagaaaaac acgcaggaaa tttaacaaga gcagctgttg agcttgctaa
      181 agattggcgt acagataaat atctcagaag attagaagct ttaatggcaa taggagataa
      241 agaaaaaagt tttattattt ctggaactgg agatgttcta gaacctgaag ataatgttat
      301 tggtattggt tcagggggaa attatgcact agcagcagcc aaaattttaa tggaatcaga
      361 cttatcagct gaagatgttg caaggaaatc aataaaagtt gcatcagata tatgtgtctt
      421 cacaaataat aatgtaaaaa ttgaaaaaat ttaatggaaa aaaataacgt aacttcattt
      481 atacctaaag ataaaaaaaa aaaaataaaa aattcataca ttagttcttt atcaccaaga
      541 gaaatagtat ctgagcttga taggtatatt gttggacaaa ataaagcaaa aaaagctgtt
      601 gctattgctt taagaaatag atggcgtaga caagctttag aaggtgatat gaaagatgaa
      661 gtattaccaa aaaatatcct aatgattggg ccaacaggag ttggtaaaac tgaaatagca
      721 agacgtttat ctaaactagc agaagcacca tttgtaaaag ttgaagcaac tcgttttact
      781 gaggttggat atgttggaag agatgttgaa caaatagtaa gagatcttct tgaaattgca
      841 atagcaatgg aaaaagtaaa aaaaagaaaa gaagtttatg ctaacgcaca aaaatttgca
      901 gaggaaagag tcttagatgc attcgttgga aataaagcaa gtgttgcaac aagagaaagt
      961 tttagaaaaa gactaagaag tggggattta gatgataatg aaattgaaat agccattaat
     1021 gaaagcaaca gtatgccttc atttgaaata cctggcatgc caggagcaaa tgtgggaatg
     1081 attaacctga gtgatatgct tg
//
LOCUS       DTVV01000412            1190 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2229475, whole genome shotgun sequence.
ACCESSION   DTVV01000412 DTVV01000000
VERSION     DTVV01000412.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2229475"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="EYO86_04895"
     CDS             <1..170
                     /locus_tag="EYO86_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIC42401.1"
                     /translation="GEKLKVKGVTKSKFLLISGKPIGEPIARGGPFVMNTKEEILKAV
                     EDYHSGNFVQK"
     gene            167..1141
                     /locus_tag="EYO86_04900"
     CDS             167..1141
                     /locus_tag="EYO86_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HIC42402.1"
                     /translation="MTRVVIIENTGGPEVLKFETAKLNSPTPEEVLIEHKAIGLNFID
                     IYHRSGLYPLELPSGIGGEASGVIKEIGSKIKDFSVGDNVAYAGAPLGSYSSERNYPA
                     KNLVKIPSGVNFDVAATLMTKGLTTYYLLCKTYPVSANETILFHAAAGGVGQIFCQWA
                     KSLGCKVIGTVGSDEKISIAKKNGCDFVINYSKENFPKKVLEITKGKGVPVVYDGVGK
                     DTFAGSIECLKIRGMLVSFGNSSGPLSEIDVKKMIQPKGLYFTRPSMAQYLGTKDQIK
                     EGADKLFEKIKLGQIKVKIFKKYKLEDAIQAHEDLEARKIIGPAIIIP"
BASE COUNT          446 a          162 c          224 g          358 t
ORIGIN      
        1 agggtgaaaa attgaaagtt aaaggtgtaa ctaagtcaaa atttttacta atatctggaa
       61 aacctattgg agaacctata gctagaggtg gaccatttgt tatgaatact aaagaagaaa
      121 tacttaaagc tgttgaagat taccatagcg gaaattttgt acaaaaatga ctagagtagt
      181 aataattgaa aacactggtg gacctgaagt tcttaaattt gaaaccgcca aactaaatag
      241 tccaacacca gaagaagtat taattgagca caaagctata ggtttaaatt ttatagacat
      301 ttatcatcgt tcaggtttat acccactgga attaccatca ggaataggtg gtgaagcctc
      361 aggtgttatc aaagaaattg gatcgaaaat taaagatttt tcagttggag ataatgtagc
      421 ctatgctggt gcacctctcg gatcatattc gtctgaaaga aattaccctg caaaaaattt
      481 agtaaaaata ccgagtggtg taaattttga tgttgccgct actttgatga ccaaaggttt
      541 aactacatac tacttgctat gcaaaactta tcctgtctca gctaatgaaa ctattttatt
      601 ccatgcagcg gctggaggtg tcggccaaat tttttgtcaa tgggcaaaaa gtttaggttg
      661 taaagttata gggactgttg gttcagacga aaagattagt atagccaaaa aaaatggttg
      721 tgattttgta ataaattatt ctaaagaaaa ttttccaaaa aaagttttag aaattactaa
      781 gggaaagggt gttcctgttg tttatgatgg tgtgggcaaa gatacttttg caggatcaat
      841 tgaatgttta aaaataagag gaatgttggt ttcttttggt aattcatctg gtcctctttc
      901 agaaatagat gtgaaaaaaa tgatacagcc caaaggtctt tattttacaa gaccttctat
      961 ggctcaatat ctgggaacaa aagatcaaat aaaagaagga gctgataaat tatttgaaaa
     1021 aattaaattg ggccaaataa aagtaaaaat atttaaaaaa tataaattag aagatgccat
     1081 tcaagcccat gaagatttgg aagcaagaaa aatcataggt ccagcaatta ttattcctta
     1141 atggaaaaat tccaaatatt tattcaactc actagactaa ataaacccat
//
LOCUS       DTVV01000413            1146 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2229618, whole genome shotgun sequence.
ACCESSION   DTVV01000413 DTVV01000000
VERSION     DTVV01000413.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2229618"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="EYO86_04905"
     CDS             <1..257
                     /locus_tag="EYO86_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIC42403.1"
                     /translation="AINREWQCGTVQVDFNLPERLNANYINEKGEKQKPVLIHRAIFG
                     SLERFLGILIEHYNGKFPFWLAPTQVTICTITEKFNPLSF"
     gene            complement(772..>1146)
                     /locus_tag="EYO86_04910"
     CDS             complement(772..>1146)
                     /locus_tag="EYO86_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIC42404.1"
                     /translation="LKISNAVKTDSNEVVLWGDGSPTRDFLFVDDAADGIILAAQKYE
                     DGTPINLGAQKETSIKELAMLVSEIMRFSGDIIWDNTKANGQPRSCVSNYKAEREIGF
                     KPKISLKEGLTKVISWFESNYS"
BASE COUNT          431 a          176 c          157 g          382 t
ORIGIN      
        1 atgcaattaa cagagaatgg caatgtggga cagttcaagt agattttaat ttaccagaaa
       61 gattaaatgc aaattatata aatgaaaagg gagaaaaaca aaaaccagtt ttaatccatc
      121 gtgcaatatt tggatcttta gaaagatttt taggtatttt aattgaacat tacaatggaa
      181 aatttccatt ttggttagct cctactcaag ttacaatatg tactattact gaaaaattta
      241 atccacttag tttttgacaa tggcagtcat gaatctactg gaggacaacc aacctgtaca
      301 aattctatta gtattgcaaa aattgctaaa gcggcaaatt tcaaagtttt ccaagtagaa
      361 aatgaatctc agtttgagaa gatcttagtt aaaatcaaaa aactacttgg gccaataatg
      421 atagtagtta aaataaagaa cagtaaagag gctagtggta gagtttcatt aaaaccttta
      481 gatataaaaa aaagatttac ttccaaatga aaaactatga ataactttaa tgtggcgtta
      541 agcatatgaa taaaattttt acattaaaag cggactctct taaaatacta caaagaaaaa
      601 ttaaaaattc taaaaaagaa aaagtatttt tgaaatatac tccactagag atcaaataat
      661 ctaagaatgg agtatgtgat gttttatcta caccaaaaca ttgatcatgt cttaatccat
      721 caactaaaat aaataatata tttggatgca tgttacaact cttcatatta gttaactgta
      781 attagattca aaccatgaaa ttactttagt tagaccttct tttaaactaa tttttggttt
      841 gaatccaatc tctctttctg ccttataatt actaacacag cttcttggct gtccattagc
      901 ttttgtatta tcccatataa tgtcgccaga aaatctcatt atttctgaaa caagcatggc
      961 taattctttt attgatgttt ctttttgagc acctaaatta attggtgtac catcttcata
     1021 tttttgtgct gcaagaataa taccatcagc cgcatcatct acaaacaaga aatctcttgt
     1081 tggtgaacca tctccccata acacaacctc attagaatca gtttttactg catttgaaat
     1141 ttttaa
//
LOCUS       DTVV01000414            1418 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2232448, whole genome shotgun sequence.
ACCESSION   DTVV01000414 DTVV01000000
VERSION     DTVV01000414.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2232448"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="EYO86_04915"
     CDS             <1..313
                     /locus_tag="EYO86_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIC42405.1"
                     /translation="YIKKIFSKNKTTFCYCISEYPADMNKIDWKNAIKFDGFSDHALG
                     IIAPIIFAVLKKQQKSKNILIKKHVKLNNSSGSDAGSSIDTEELSELVKVIRQIERLR
                     I"
     gene            complement(316..1392)
                     /locus_tag="EYO86_04920"
     CDS             complement(316..1392)
                     /locus_tag="EYO86_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIC42406.1"
                     /translation="MIKLFEPHVGIEEIKSVENVLKSKFWASGAGAGKVLEFEKKFKK
                     YVKSNTCIAVNSGTAALNLAFSLFDIKNKEVILPSLSFISTAHCIVENGGIPIFVDIE
                     PDTLCIDPEKIKQAISKDTTAILPVHFGGMPCNLNEIRKIAKSHNLHIVEDAAHAVGT
                     EFNGKKIGSHGTAVCFSFHPVKNLAMPTGGLISINHKNHEKFKQLLFSRRWCGITNRR
                     ETDYDVKELGWNYYMNEFSAALGLLQLKKVDKLNKIRRKIAKKFCAEINIKSKMQFNE
                     NCSYHLYWILVKNRQKFRQKLHKNGIETGTHYKPIHLMKMYKDNISLPITESIGRQIV
                     TIPIHPNLKKSEIEKIIKFVNNYA"
BASE COUNT          481 a          218 c          186 g          533 t
ORIGIN      
        1 atatatcaaa aaaatatttt caaagaataa aactactttt tgttattgta tatctgaata
       61 cccagcagat atgaataaaa ttgattggaa aaatgcaata aaatttgatg gtttttctga
      121 tcacgcatta ggaattattg caccaattat ttttgcagtg ttaaaaaaac aacaaaaatc
      181 aaagaatatt ctaattaaaa aacatgtaaa attaaataat tcgagtggat ccgatgctgg
      241 ttcatcaatt gatacggaag aattatcaga attagttaag gtaatacgcc aaattgaaag
      301 actgagaata taaaattaag cataattatt aacaaactta attatttttt caatttcaga
      361 ttttttaaga ttaggatgaa ttggtattgt aacaatctgt cttcctattg attcagttat
      421 aggtaacgaa atattatctt tgtacatttt cattaaatga ataggtttgt aatgtgtccc
      481 tgtttcaatt ccatttttat gtaatttctg tcgaaatttt tgacgatttt ttaccaaaat
      541 ccagtataag tgataagagc aattttcatt gaattgcatt tttgatttaa tgtttatttc
      601 agcgcaaaac ttttttgcaa tttttcttct aattttattt aatttatcta ctttttttaa
      661 ttgcagtaat cctagtgcag cagaaaattc attcatgtag taattccaac ctaattcttt
      721 aacatcataa tccgtttctc ttctattagt aattccgcac catcttcgtg aaaataataa
      781 ttgcttaaat ttttcatggt ttttatgatt aatagaaatc aatcctcctg ttggcattgc
      841 aagattcttc acaggatgaa aactaaaaca aacagctgtt ccatgagaac caattttttt
      901 cccattaaat tctgtgccaa ctgcatgagc ggcatcttcg acaatatgta aattatgaga
      961 ttttgcaatt ttacgtattt catttaagtt acaaggcata cctccaaaat gaacaggcaa
     1021 aatcgctgtt gtatcttttg aaattgcttg tttaattttt tctggatcaa tgcaaagagt
     1081 atcaggctca atatctacaa aaataggaat tccaccattt tcaacaatac agtgagcagt
     1141 tgagataaaa ctcagagaag gcagaattac ttctttattt tttatatcaa ataaggaaaa
     1201 tgctagattt aatgctgcag ttccactatt tactgcaata catgtatttg atttcacata
     1261 ttttttgaat tttttttcaa actcgagtac ttttcctgcg ccagctccag acgcccagaa
     1321 cttgcttttc aaaacatttt caacagattt tatttcctca attccaacat gcggttcgaa
     1381 taatttgatc attttcttat aatcatactt ttttaacc
//
LOCUS       DTVV01000415            1157 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2249035, whole genome shotgun sequence.
ACCESSION   DTVV01000415 DTVV01000000
VERSION     DTVV01000415.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2249035"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="EYO86_04925"
     CDS             <1..900
                     /locus_tag="EYO86_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC42407.1"
                     /translation="NPSNFQLHLMIMIFMYAVMAQSWNVIAGFSGQISLGHVIFFGIG
                     GYTSSFFYVEYGISPWIGIIIGILFSSLVAIAIGIPMLRLSGHYFAIATLLIGFSFQI
                     IFQRWDLIGAASGLWIPLNWDNPWLALQFHESKIPYYYIFLIVFLLSYFYIWILYRSK
                     IGFKLRAIRDDPQAATSLGIPVAKYKIIAFVISAILMSPMGSLFAQYILVIEPARMFD
                     IEISIIVLLIAVMGGVGNVWGPLIGTIILIPLSEYTRVFFGGTGGSVDLIIYGILIML
                     ICIFRPNGLISFFPKNILERKKF"
     gene            900..>1157
                     /locus_tag="EYO86_04930"
     CDS             900..>1157
                     /locus_tag="EYO86_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017969891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC42408.1"
                     /translation="MTNILEVKNLSKSFGGIIANNNISFEANSGEIIGIIGPNGAGKS
                     TLFDLITGYTKPDTGGVKFNGQNILNLRPDLISSLGIGRTFQ"
BASE COUNT          353 a          157 c          203 g          444 t
ORIGIN      
        1 aatccttcaa attttcaact acacctaatg attatgattt ttatgtatgc tgtaatggca
       61 caatcatgga atgttatagc aggtttttct ggtcaaataa gcttaggtca tgttattttt
      121 tttggaatag ggggttacac aagctctttt ttttatgtag agtatggcat ttctccttgg
      181 ataggaatca tcataggaat tttattttct tctctagttg ccatagcaat tggaattccg
      241 atgttaagac tttctggaca ttattttgct attgccacac tgttaattgg atttagtttt
      301 cagattattt tccaaagatg ggacttaata ggtgcagctt caggtctttg gattccttta
      361 aattgggaca acccttggtt agcattacag tttcatgaaa gtaaaatccc ttattattat
      421 atttttctaa ttgttttttt attatcttat ttttatattt ggatccttta tagatcaaaa
      481 ataggattta agttacgagc cattcgagac gatcctcaag cagcgactag cctaggcata
      541 ccagttgcaa aatataaaat tatagctttt gtaatttctg caattttaat gtctccaatg
      601 ggaagtttat ttgcacaata tattttagtg attgaaccag caagaatgtt tgacattgaa
      661 atctctataa ttgtcttgct aattgcagtt atgggtggcg ttggtaatgt ttggggtcca
      721 ctaataggaa caataatttt aattccgctt tcagaataca ctagagtatt tttcggggga
      781 acaggaggat cggttgatct aataatttat ggtattttaa taatgttaat atgtattttt
      841 cgacctaatg ggctaatttc tttttttcca aaaaatattc ttgaaagaaa aaaattttga
      901 tgactaatat cttagaagtt aaaaatttaa gtaaatcctt tggtgggata attgcaaata
      961 ataatatctc ttttgaagct aattcaggag aaattattgg tattatagga cccaatggtg
     1021 ctggaaagtc tacattattc gatttaatta ctggctatac gaaacctgat acaggaggag
     1081 ttaaatttaa tggtcaaaac attcttaatt tacgtccaga tttaatatct tctttaggta
     1141 ttggaagaac atttcaa
//
LOCUS       DTVV01000416            4540 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2257446, whole genome shotgun sequence.
ACCESSION   DTVV01000416 DTVV01000000
VERSION     DTVV01000416.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2257446"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="lpxK"
                     /locus_tag="EYO86_04935"
     CDS             <1..957
                     /gene="lpxK"
                     /locus_tag="EYO86_04935"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:PF02606.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIC42409.1"
                     /translation="NFLYDYSFWKPKKSKAKVISVGNLSLGGAGKTPFTIALSKFLIS
                     KNYSVGIVVTGHGRNNNNFFFGQSFLMENQSWEDAGDETVLLKNNLNDVPIYVSWRKK
                     NKVHGVNYLSEKNNCDIVILDDAFQHRKIYRDLDIVLISSDNLTDSQKMFPYGLLREP
                     ILGLKRADMIIETKGNLFNEANRNQNRFILHWEFNDCLLMPNDETCAISALEKYNNIL
                     SLCAIGNPKSFKKTLELLKITPKKSLVYPDHYPFKKRDIYKINQFINKNAVDAILCTE
                     KDLIKLKQYKKMIKIPVGAITINYCLNSEIEREILSKIKEVF"
     gene            1023..1628
                     /locus_tag="EYO86_04940"
     CDS             1023..1628
                     /locus_tag="EYO86_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIC42410.1"
                     /translation="MVELFLDGGPFMYPILLLLLGGLALVVERFRSLSQSQADSDGFM
                     KDLSKAVKAGGSKGGMVVCKGHDGPVSNICKAGLDKAGKGADRVEKAIENAGSLEMAF
                     LEKNMGWLTAIVAIAPMMGFTGTVWGMIGAFEAIEAANDISPAVVAGGISQALLTTAF
                     GLVVAMIIQICQNFFSSKIDEMVLDMEEKSIELTDLLRSAK"
     gene            1632..2030
                     /locus_tag="EYO86_04945"
     CDS             1632..2030
                     /locus_tag="EYO86_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIC42411.1"
                     /translation="MALRERKTRVGAIPEGGMADIAFLLLVFFLVTTTMDMDKGIGII
                     LPAEGQEIEINKKNILNLLISSSGNVLLGGEAIAINQIDDRVKEEIRKNDKLIISVKA
                     HEKTQYDVYIAVLDQLKKAKATRISIAETE"
     gene            2027..2443
                     /locus_tag="EYO86_04950"
     CDS             2027..2443
                     /locus_tag="EYO86_04950"
                     /inference="COORDINATES: protein motif:HMM:PF02472.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIC42412.1"
                     /translation="MKFKRKSNIESKISTSSMPDIIFMLLIFFMVTTVLREYSGLQIS
                     LPKAERIEKLESKRHTAHIWVSKDGLISIEDKLFKSNAIRHVMYEKRASDPRLTVSLK
                     ADLAVRMEVISSIHNELRKADALRLNYSTKVKAPSQ"
     gene            2440..3111
                     /locus_tag="EYO86_04955"
     CDS             2440..3111
                     /locus_tag="EYO86_04955"
                     /inference="COORDINATES: protein motif:HMM:PF03544.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIC42413.1"
                     /translation="MNKINPISFIVRLVLNRANLNDGTLKEQYSTVTRIGAVLACLLG
                     LFSFYGLQRFDPDIDIESEGQIIIENIEIPETQQFETPPPPARPSIPIESEDEDLADD
                     LTIEETDLDNFDAWDAPPPPPSGPQFKFIPYDDPPRPITPIKPVYPDIAQEAGIEGQV
                     LIQCFIDKTGRVKETIVIKGVPNTGLNESAVAALRKTRFRPARQRENPVGVWITIPIN
                     FKLQN"
     gene            complement(3196..3933)
                     /locus_tag="EYO86_04960"
     CDS             complement(3196..3933)
                     /locus_tag="EYO86_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIC42414.1"
                     /translation="MNINLKVWRQEKDTKKGMFKTYRLQDIDPKISFLEMLDVLNNKL
                     IKQKLDPVAFDHDCREGICGSCGFMINGQPHGPQKATTVCQLYMRFFNSENEIVLEPF
                     RARSFKIIKDLSIDRTSFDRIIYAGGFVPVDTGDAEEANSTIVEKANADEAFLSAACI
                     GCGACVAACKNSSAMLFTAAKVSHLALLPQGEPDKKERVQKMVATMDEEGFGACTNTG
                     ACSAECPKEISQENIARLNREFIKSQL"
     gene            complement(3933..4523)
                     /locus_tag="EYO86_04965"
     CDS             complement(3933..4523)
                     /locus_tag="EYO86_04965"
                     /inference="COORDINATES: protein motif:HMM:PF02910.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42415.1"
                     /translation="MQALADGYFIVPHTITDYLSKETPGSLNKTNQSTFESVAHNTKQ
                     EIEKIMNIKGTLVVDEIHKELGKIVWDKVGMSRNSEKLRKALEEIPKIKEKFWTKLLI
                     PGSKEDFNQELEKALRLKDFIELAELMALDALERKESCGGHFREEFQTKENEAKRNDI
                     EFMYVAAWQHNKDGKPKLFKEGLEYKNVKLKTRSYK"
BASE COUNT         1434 a          791 c          843 g         1472 t
ORIGIN      
        1 aattttttat atgactacag tttttggaag ccaaaaaaat caaaagccaa agttattagc
       61 gtcggaaatc tttcgttggg tggcgctggg aaaacgcctt ttaccattgc tttatcaaag
      121 tttttaattt caaaaaatta ttccgtgggc attgttgtta caggacatgg aagaaataat
      181 aataattttt tcttcggtca aagcttcctg atggaaaacc agtcatggga ggatgcagga
      241 gatgaaactg ttttgttaaa aaataattta aacgatgtgc ctatctatgt ttcttggcgg
      301 aaaaaaaaca aggtacatgg agtaaattat ctttctgaga agaacaattg tgatatagta
      361 atattagacg atgcttttca gcatcgtaaa atttatagag accttgatat tgtattaatt
      421 tcttctgata atctcacaga ttcgcaaaaa atgtttccat atggcctatt gcgtgaaccc
      481 attttaggtc ttaaaagagc tgatatgatc atagaaacaa aaggaaatct gtttaatgaa
      541 gccaacagaa atcaaaatcg gtttattctt cattgggaat tcaatgattg tcttttgatg
      601 cccaatgacg aaacgtgtgc aataagcgct ttagaaaaat acaacaatat tttatctttg
      661 tgtgcaattg ggaaccctaa aagctttaaa aaaacactag aactcttaaa aataacccct
      721 aaaaaatctt tagtatatcc agatcattat ccgtttaaaa aaagagatat ttataagatt
      781 aatcaattta taaataaaaa tgctgtggat gctattttgt gcacagaaaa agacctaatt
      841 aaactaaaac aatataaaaa aatgattaaa attccagttg gagctataac cattaattat
      901 tgtttaaatt cagaaattga aagagaaatt ttatcaaaaa taaaggaagt tttctagttt
      961 attgacattc cctttgttat aagctaagtt tttacaaatt taacaggtta ggaggagctt
     1021 aaatggtaga actattttta gacggtgggc cttttatgta ccctatttta ttactattgc
     1081 ttggaggttt ggctttggtg gttgagcgtt ttcgttcttt atcacaatca caggctgatt
     1141 ctgatgggtt tatgaaagac ttatcaaagg cagtaaaagc tggtggatca aaaggcggaa
     1201 tggttgtttg taaaggacat gacggtcctg tttcaaacat ctgtaaagct ggtttagaca
     1261 aagctggcaa aggagctgat cgggtagaaa aggcaattga aaatgcaggg tctcttgaaa
     1321 tggcttttct tgaaaaaaat atgggttggt taactgcaat tgttgcaata gcgccaatga
     1381 tgggatttac tggtactgtg tggggaatga ttggtgcttt tgaagcaatt gaagcagcaa
     1441 acgatatttc tccggctgtt gttgctggcg gtatttcaca ggccttgcta accactgctt
     1501 ttggtttggt tgttgctatg ataatccaaa tttgtcaaaa ctttttttct tctaaaattg
     1561 atgaaatggt tttagacatg gaagaaaaat caatcgaact aacggacctt cttagaagcg
     1621 caaaataaaa aatggctctt agagaaagaa agaccagggt aggagctatc cctgagggcg
     1681 gaatggcaga cattgcattt ttgcttttag ttttcttttt ggtaaccacc acaatggaca
     1741 tggacaaggg cattggaatt attctgccag cagaaggaca agaaattgag attaataaaa
     1801 agaatattct taatttgctt attagttcct ctggaaatgt tcttttgggc ggagaagcaa
     1861 ttgccataaa tcaaatagat gatagggtca aagaagaaat aagaaaaaat gacaaactta
     1921 ttatttcggt caaggcacat gaaaaaacac aatatgacgt atatattgcg gttttagatc
     1981 aattaaaaaa agctaaggct acacgcatat caatagcaga aacagaatga agtttaaaag
     2041 aaaatcaaac atagagtcaa agatatccac ttcttccatg ccggatatta ttttcatgct
     2101 tcttatcttt tttatggtta caaccgtatt aagagaatat tctggtttac aaatttcgct
     2161 tccgaaagca gaacgtattg aaaaattaga atcaaaaaga catactgcac acatttgggt
     2221 atccaaagac ggattaatct ctattgaaga caaattgttt aaaagcaatg ctattcgtca
     2281 tgttatgtac gaaaaaagag cttcagaccc ccgactaact gtttcattaa aggcagattt
     2341 ggctgtaaga atggaagtta tttcgagcat acataacgaa ctaagaaagg ccgatgctct
     2401 tagattgaac tattctacaa aagtaaaggc tccgagccaa tgaacaaaat aaatcctatt
     2461 tcctttattg taaggcttgt tctaaaccgc gcaaacctca acgacggtac tttaaaagaa
     2521 caatactcaa cggttacaag aattggtgcg gtgttggctt gccttcttgg tttgttcagt
     2581 ttttatggat tacaacgatt tgatcccgac attgatattg aaagtgaagg acaaattatt
     2641 atcgaaaaca tcgagattcc tgaaacacaa caatttgaaa cccctcctcc gcctgcaagg
     2701 ccttctattc caattgagtc ggaagacgaa gacttagcag atgatttaac aattgaggaa
     2761 acagatttag acaattttga tgcgtgggac gctccaccgc ccccaccttc tggaccacaa
     2821 tttaaattta ttccatacga cgaccctcct cgtccaatta caccaatcaa acctgtatat
     2881 ccagacattg ctcaagaggc ggggattgaa ggacaggtgt taattcagtg ttttattgat
     2941 aaaaccggaa gggtcaaaga aactattgta atcaaaggcg ttccaaatac tgggctaaac
     3001 gaatctgcag ttgctgctct tagaaaaaca aggtttagac ccgctagaca gcgtgaaaac
     3061 ccagtgggag tatggattac tattccaatt aattttaaac tccaaaacta aaaaggctga
     3121 cattgttcag ttaatgtttt tttcttcttg agaagcgtgt tcaaaatacc gttcacagca
     3181 tttttattaa aaactttata gttgagattt aataaattcc ctattaagtc tcgcaatgtt
     3241 ttcttgtgaa atttctttag gacattcagc tgaacaagct ccggtgtttg tgcaagcccc
     3301 aaacccttct tcgtccattg tggccaccat tttttgaacc ctctcttttt tgtcaggctc
     3361 tccttgtggc aacaaggcaa ggtgtgaaac tttggctgcc gtaaacaaca ttgctgaaga
     3421 atttttacag gcagcaacgc atgccccaca accaatacag gccgcagaaa gaaaggcttc
     3481 atcagcattt gctttttcaa caatggtcga gttagcttct tctgcgtctc cggtatcaac
     3541 aggaacaaat cctcctgcat aaataatgcg gtcaaaagaa gttctatcaa ttgacaggtc
     3601 tttaataatt ttaaatgatc ttgccctgaa gggttccaaa acaatttcgt tttcactgtt
     3661 aaaaaatctc atataaagct gacaaaccgt tgtagctttt tggggtccgt gtggctgtcc
     3721 attaatcata aaaccacagg aaccacagat tccctcgcga caatcgtggt caaaagctac
     3781 cggatctagt ttttgcttga taagcttgtt gtttaaaacg tctaacattt ctaaaaagga
     3841 gatttttggg tctatgtcct gaagccgata tgttttaaac atgcctttct ttgtgtcttt
     3901 ttcttgtctc caaactttta agttaatatt cattatttat agcttcttgt ttttagctta
     3961 acgtttttat actctaaacc ctctttaaat agttttggtt ttccgtcttt gttgtgttgc
     4021 catgccgcaa catacataaa ttctatgtcg tttctttttg cttcattttc ttttgtttga
     4081 aattcttccc taaagtgtcc accgcaagat tcttttctct ctagcgcatc aagggccata
     4141 agctctgcca attcaataaa gtctttgagt cttagggctt tttctagctc ttggttaaaa
     4201 tcttccttag agccaggaat taataatttt gtccaaaatt tttcttttat ttttgggatc
     4261 tcttcaagcg cttttcgcag tttttcgcta tttctagaca ttcctacctt atcccaaaca
     4321 atttttccta attctttatg aatttcatca acaacaaggg ttccttttat attcataatt
     4381 ttttcaattt cttgttttgt gttatgtgct acgctttcaa acgttgattg attagttttg
     4441 tttaaagaac cgggtgtttc ttttgaaaga tagtcggtga ttgtgtgggg gacaataaaa
     4501 tacccgtctg caagcgcttg cattagcgcg cttgctccta
//
LOCUS       DTVV01000417            1099 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2258895, whole genome shotgun sequence.
ACCESSION   DTVV01000417 DTVV01000000
VERSION     DTVV01000417.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2258895"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1099
                     /locus_tag="EYO86_04970"
     CDS             <1..>1099
                     /locus_tag="EYO86_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIC42416.1"
                     /translation="KLMEHINNEMNVISVEKRIRGRVKNQMEKTQREYYLNEQMKAIQ
                     RELGEIEDGGDETGQLQKSIIKAKMTKEATKKCLSELKKLKSMSSMSAEATVVRNYLD
                     WMIELPWNSKENQLGKVNIDEAKRILDEDHYGLEKVKERILEYLAVQKRVGKIKGAII
                     CLVGPPGVGKTSLGKSIARATGRKFVRMSLGGIRDEAEIRGHRRTYIGSLPGKIIQQM
                     KKAGTKNPLFLLDEIDKVGTDYRGDPSSALLEALDPEQNVTFNDHYLEVDYDLSDVMF
                     VTTANTLNILPPLLDRLEVIRIPGYTEDEKINIANNYLIPKQIKNNGLKNEEWKLDKD
                     VIKKVIQSYTKEAGVRNLEREISKLARKTVK"
BASE COUNT          466 a          134 c          222 g          277 t
ORIGIN      
        1 aaaaactaat ggaacatatt aataacgaaa tgaatgttat tagtgtagaa aaaagaataa
       61 gaggtagagt aaaaaaccag atggaaaaaa cgcaaagaga atattattta aacgaacaaa
      121 tgaaagctat tcaacgagag ttaggtgaaa ttgaagatgg tggagatgaa actgggcaat
      181 tacaaaaatc aattattaaa gcaaaaatga ctaaagaagc tacaaaaaaa tgtttatcag
      241 agttaaagaa attgaaatca atgagttcaa tgtctgctga agccacagtt gtaagaaatt
      301 atctcgattg gatgattgaa ttaccttgga acagtaaaga aaatcaatta ggaaaagtga
      361 acattgatga agcaaaaaga atattagatg aagatcatta tggtctagaa aaggtaaaag
      421 aaagaatatt agagtatctg gctgtgcaaa aaagagttgg caaaataaaa ggtgctataa
      481 tatgtcttgt aggccctcct ggtgtcggca aaacctcact tggaaaatct atagctagag
      541 ctacgggaag aaaatttgtt cgtatgtctc taggaggaat aagagatgag gctgaaatca
      601 gaggacacag aagaacttat ataggttcat tacctggaaa aattatacaa caaatgaaaa
      661 aagctggaac aaagaatccg ctttttttgc tagatgagat tgataaggtc ggtactgatt
      721 atagaggaga tccttcgtca gcattgctag aagcgctaga ccctgagcag aatgtaacct
      781 tcaatgatca ctatcttgaa gttgattatg atctatctga tgtgatgttt gtaactacag
      841 caaatacttt aaatatttta ccaccactac ttgacagatt agaagttatc agaataccag
      901 gatatacaga ggatgagaaa attaatattg cgaacaatta tttaatacca aaacaaatta
      961 aaaataatgg acttaagaat gaagaatgga aactggacaa agatgttatt aaaaaagtaa
     1021 tacaaagcta cacgaaagaa gctggcgttc gtaatcttga aagagaaata tctaaattgg
     1081 caagaaaaac tgtaaaaag
//
LOCUS       DTVV01000418            1408 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2260454, whole genome shotgun sequence.
ACCESSION   DTVV01000418 DTVV01000000
VERSION     DTVV01000418.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2260454"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /gene="uvrC"
                     /locus_tag="EYO86_04975"
     CDS             <1..1246
                     /gene="uvrC"
                     /locus_tag="EYO86_04975"
                     /inference="COORDINATES: protein motif:HMM:PF02151.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HIC42417.1"
                     /translation="FLGGNTKKIESKLKKLMLKASSSQMFEEAGRLRDRIKSINQIQK
                     YQSVYIRDMRNIDIFAIKILEGKSCIHGKFFRNGSNYGNKSFFPVHDINAQENEILES
                     FLYQFYADKDVPPKILINKNPKLFKDVEKILNIKNKLKTKIIQPQTGEKFRHIQLAEK
                     NASENIKLKKASLENHHEALKKLIKLFNLNEVPNRIEVYDNSHTFGKESVGVMIVVDS
                     EGFSPKNYRKYNIRYDDKATNPASVDDYYMMKEVLSRRLKTKKDKEGVIMPDIIIVDG
                     GRGQFNAVAKVLNDNQRDTISLMSVSKGKERNAGREIIHLNNRSLTLQPNDPLLFFIQ
                     RIRDEAHRFAITTHRSRRGKQSVQSVFDELPGIGPKRKKMLILHFGSIQKIKNATKTD
                     LGSVKGIPKRTIDQVYDFFHSH"
     gene            1275..>1408
                     /locus_tag="EYO86_04980"
     CDS             1275..>1408
                     /locus_tag="EYO86_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIC42418.1"
                     /translation="MKLNLSNILTICRILVIPIIVALIYLKSPFYGWLAFILFCLASI
                     T"
BASE COUNT          534 a          228 c          250 g          396 t
ORIGIN      
        1 atttttaggt ggaaacacca agaaaattga atcaaaatta aaaaaattaa tgcttaaggc
       61 aagttccagt caaatgtttg aagaagcggg acgactacgt gatcggatta agtccattaa
      121 tcaaattcaa aaatatcaat cagtgtatat tagggatatg cgcaacattg atatttttgc
      181 cattaaaatc ctagagggga aaagttgcat tcatggaaaa ttttttcgta atggcagtaa
      241 ctatggcaat aaatcgttct ttcccgtgca tgacattaat gctcaagaaa atgaaatttt
      301 ggaatctttt ttatatcaat tttatgcgga taaggatgtg ccgccaaaaa ttctcatcaa
      361 taaaaaccct aaactgttta aagatgttga aaaaatactg aatattaaaa acaaactaaa
      421 aacgaaaatc atacaaccgc aaacagggga aaagttccgt catatccaat tagcggaaaa
      481 aaatgccagt gaaaacatta aactaaaaaa agcaagttta gaaaaccatc acgaagctct
      541 aaaaaaatta ataaaattat tcaatttgaa tgaagtacct aaccgcattg aagtatacga
      601 caatagtcac acctttggta aagagtctgt tggggtaatg attgtggtag atagtgaggg
      661 ctttagtcca aaaaattatc gtaaatataa tattcgttac gatgataagg caaccaatcc
      721 cgcaagtgtt gatgattatt acatgatgaa agaagtttta agtcgacgat taaaaacaaa
      781 aaaagataaa gaaggagtca ttatgcctga cattatcata gtggatggag gacgaggtca
      841 gttcaatgca gtcgcaaaag tattgaatga taaccagcgg gatacaattt ctttaatgag
      901 tgtttccaag ggaaaagaac gaaatgctgg acgcgaaatt atccacctta ataacagaag
      961 cttaacattg caaccaaacg atccactttt attttttata cagcggatac gtgatgaagc
     1021 gcatcgtttt gccatcacaa cgcatcgctc acgacgagga aaacagtccg ttcaatccgt
     1081 ttttgatgaa ttgccaggaa ttggacccaa gcgaaaaaaa atgctgatac tgcactttgg
     1141 ttctatacaa aaaataaaaa atgccacaaa aacagacttg ggatcagtaa aaggtatacc
     1201 taagcgaacc atagatcaag tttatgattt ttttcatagt cattaattta aacttagtct
     1261 aaaaaatcaa ttggatgaaa ctaaatctat caaacattct caccatatgc cgcatattgg
     1321 taatcccaat tatcgttgcg ttgatatatt taaagagccc attttatggt tggttggctt
     1381 ttatcttatt ctgtcttgcc agcataac
//
LOCUS       DTVV01000419            2164 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2260480, whole genome shotgun sequence.
ACCESSION   DTVV01000419 DTVV01000000
VERSION     DTVV01000419.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2260480"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="EYO86_04985"
     CDS             complement(<1..374)
                     /locus_tag="EYO86_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIC42419.1"
                     /translation="MLNINLKRFKFLHKKKQNQIIFSSIETTGEKEILNLIDNFLIDK
                     NSFVFESVEKGIIKGRYTIFGKNPDKIWEFNNNSCYKYDDKKNKIKINGRPKNIIEKI
                     IEEFKFKIPKTLPPISSLLSGY"
     gene            complement(374..1798)
                     /locus_tag="EYO86_04990"
     CDS             complement(374..1798)
                     /locus_tag="EYO86_04990"
                     /inference="COORDINATES: protein motif:HMM:PF13624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA"
                     /protein_id="HIC42420.1"
                     /translation="MLSTLRSFSKGKLATILVTIIIIPFVFWGMGSVFRGGKTNSVAK
                     INSFNISTKDLVDHINNSKLSADKIKENINNNILEEFLTELVSNSVIDIEIEDLNIMI
                     SDKNLAERIKKNKSFHDEKNKFSRLKYEKFLLENNIPAAVFEIGLRKNELKKEFFTYI
                     SGGIKSPYFIANKTFKDNAKKIDLSYVNLNHVYKKKDEFSNIEIETFIKDNEEKLKIE
                     IIDFSYTKITPLNLVQEKEFSENFFSNIDEIENQVLNDTKIKEIATNFGLKISEVNNF
                     DDRNNNNLIFKQIYVKRNQDKIQIIDKNDFYLLYEINSIKKVLPERNDVKFIESVKNS
                     LFEKNKYEYNKNLLLKIQNQNFNNFDFIRLVDNKDLIKTTKINSINDNNLFTSDSIKL
                     LYSLSKNNFLLISDKDKNIYLAKIEKILSNDLSDKNDLSNYTIKANIQIKNDLYSSYD
                     YLINDKYKITINQNTLERIKNYFR"
     gene            1872..>2164
                     /locus_tag="EYO86_04995"
     CDS             1872..>2164
                     /locus_tag="EYO86_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triosephosphate isomerase"
                     /protein_id="HIC42421.1"
                     /translation="MYFIANWKMYGDFKSINSIKNVIKLSKKPKYRKAKIIYCPPYTL
                     LDQFVKITSKTKIYIGAQNCHAHQDYGAFTGSINTKMIKNTGSKFVIVGHSENR"
BASE COUNT          757 a          244 c          206 g          957 t
ORIGIN      
        1 aaatatccag acaataatga acttattggt ggtaaggttt ttggaatttt aaatttaaat
       61 tcttcaatta ttttttcaat aatatttttt ggtctaccat taatttttat tttatttttt
      121 ttatcatcat atttgtaaca actattgtta ttaaattccc atattttatc aggatttttt
      181 ccaaaaatag tatatcttcc tttaattata cctttttcaa ctgattcaaa aacaaaactg
      241 tttttatcaa ttagaaaatt atcaataagg tttaatattt ctttttctcc agtagtttcg
      301 attgatgaaa aaattatttg attttgtttt tttttatgta gaaatttaaa tctttttaaa
      361 ttaatattta atatcatcta aaatagtttt taattctttc aagtgtattt tgatttattg
      421 ttattttgta tttatcatta attaaataat cataagaact atatagatca tttttaattt
      481 gaatgtttgc ttttatggta tagttagata agtcattttt atctgaaaga tcatttgaca
      541 aaattttttc aattttagct aagtaaatgt tcttatcttt atcacttatt agtaaaaagt
      601 tatttttgga caacgaatag agtaatttaa ttgagtctga agtgaataaa ttattatcat
      661 taattgagtt aatttttgtt gtttttatta aatctttatt atctactaat ctgataaaat
      721 caaaattgtt aaaattttgg ttttgaattt ttaataatag atttttatta tattcgtatt
      781 tatttttttc aaataaacta tttttcacag attcaataaa cttaacatca tttctttctg
      841 gcaaaacttt tttgatacta tttatttcat ataataaata aaaatcattt ttgtctatta
      901 tttgaatttt atcttgattt ctttttacgt atatttgttt aaaaataaga ttattattat
      961 ttctatcatc gaaattattt acctcggaaa tttttaaacc gaaattagtt gcgatttctt
     1021 taatttttgt gtcattcaat acttgatttt ctatttcgtc aatattggaa aagaaatttt
     1081 cactaaattc tttttcttga acaagattta gaggggtaat ttttgtgtat gaaaaatcaa
     1141 ttatttctat ttttagtttt tcttcattat cttttataaa agtttctatc tcaatattac
     1201 taaattcatc cttttttttg tatacatgat ttaaattaac atatgataag tcaatttttt
     1261 tagcattatc tttaaatgtt ttattagcaa taaaatatgg agatttaatt ccaccactta
     1321 tatatgtgaa aaattctttt ttaagctcgt tttttcttaa tccaatttca aatactgcag
     1381 caggaatatt attttctaat aaaaattttt catattttaa tcttgaaaat ttattttttt
     1441 catcatgaaa acttttattt ttctttattc tttccgccaa gtttttatct gaaatcatga
     1501 tatttaaatc ttcaatttcg atatcaatta ctgaatttga aactaattct gttaaaaatt
     1561 cttcaagaat attattatta atattttctt tgattttatc ggcacttaat tttgagttat
     1621 ttatatgatc gactaaatct ttcgttgaaa tgttaaaact atttattttt gctacactgt
     1681 ttgtttttcc tccgcgaaaa acacttccca taccccaaaa tacaaatgga atgataataa
     1741 ttgtaactaa aatagttgcc aatttaccct tagaaaaact tcttaatgta cttaacataa
     1801 aaatcgttta atttattgat ctataaaaaa caaacctata aattaattta cagttaatga
     1861 caaataataa tatgtatttt atagccaatt ggaaaatgta cggagatttt aaatcgataa
     1921 attcgataaa aaatgtcatt aaactatcaa aaaaaccaaa atatagaaaa gctaaaatta
     1981 tatattgtcc accatatact cttcttgatc aatttgtaaa aattacaagt aaaacgaaaa
     2041 tatatattgg agctcaaaat tgtcatgctc atcaagatta tggagctttt actggttcaa
     2101 tcaatacaaa aatgattaaa aatactggat caaaatttgt aattgttgga cattcagaaa
     2161 atcg
//
LOCUS       DTVV01000420            1091 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2261055, whole genome shotgun sequence.
ACCESSION   DTVV01000420 DTVV01000000
VERSION     DTVV01000420.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2261055"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="EYO86_05000"
     CDS             complement(<1..266)
                     /locus_tag="EYO86_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42422.1"
                     /translation="MISKNWIEANKQVDKWKSTGLKVVFTNGCFDILHRGHVEYLTDA
                     KACGDKLETCPVCHKQMLSHDLAQVNQCMKDFIKKLCGKTQFDT"
     gene            complement(505..1050)
                     /locus_tag="EYO86_05005"
     CDS             complement(505..1050)
                     /locus_tag="EYO86_05005"
                     /inference="COORDINATES: protein motif:HMM:PF01569.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIC42423.1"
                     /translation="MKVKDYVQSHKILSDPISHFGDEMGGNWGHWILWSSIITSSKIK
                     KDSNKKFMSKIQYSTLAMVSNGLITVGMKRAFGRERPNGSCCDSFPSGHTSHSFTMAS
                     IANELYGKNIGLISYGLATLVAVSRMNDNKHYLSDVIFGAGLGTAVGRAFSKNYQKFE
                     ETNIQVGLSSQMALKISIPIN"
BASE COUNT          354 a          207 c          151 g          379 t
ORIGIN      
        1 caagtgtcaa actgtgtttt accacatagt tttttgataa aatccttcat acattgatta
       61 acttgagcta gatcatgtga taacatttgc ttatggcaaa caggacaagt ttctaattta
      121 tcaccgcatg cttttgcatc ggttaaatat tccacatgtc ccctgtgaag aatatcaaaa
      181 cagccattgg taaaaaccac ttttagtcca gttgatttcc atttatcaac ttgtttgtta
      241 gcttcaatcc aattttttga tatcatttat acatccataa ctaattcaat tctattataa
      301 aaatatttct gtaaaatact gccgtttccc tctcatgact tttatgacat ttactattac
      361 aactggtgta ttattattca ttattatctc taaatctgta actcgggttt tttgaaatta
      421 gtctatcaat tccaaaagtt gtacttgtta gaataaccat cagaatataa aacgtgcata
      481 tttcaatcaa aaaataattc tcaattaatt tatgggaatt gaaattttca aggccatctg
      541 agatgataac ccaacttgaa tatttgtttc ttcgaatttt tgataatttt ttgaaaaagc
      601 tcgcccaaca gccgtaccta atcctgcacc aaagatgaca tcgctcaaat aatgtttatt
      661 atcattcatt cgactcacag caactagtgt agccagccca tatgaaatta acccaatatt
      721 ttttccataa agttcattag caattgaagc cattgtaaaa ctatgagacg tgtgtccact
      781 gggaaatgaa tcacaacaac ttccgttggg tctttcacgg ccaaatgctc gtttcatgcc
      841 aacagtaatt aatccattgg ataccatggc aagagttgaa tattgaattt ttgacatgaa
      901 ttttttattc gaatcttttt tgattttaga tgaagtaata atggatgacc aaagaatcca
      961 atgaccccaa ttgccaccca tttcatcacc aaaatgagaa atgggatctg aaagaatctt
     1021 atgtgattga acataatctt ttactttcat atcaatttga gtggcaccca aagcagctat
     1081 tgcagccttc c
//
LOCUS       DTVV01000421            2729 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2272348, whole genome shotgun sequence.
ACCESSION   DTVV01000421 DTVV01000000
VERSION     DTVV01000421.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2272348"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="EYO86_05010"
     CDS             complement(<1..1048)
                     /locus_tag="EYO86_05010"
                     /inference="COORDINATES: protein motif:HMM:PF11412.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42424.1"
                     /translation="MLRLIKYLFFIIVIFSFTSLWALQSDWSSGNESQVRLISPISHN
                     DSSKNIYVGLEYQLQKGWKTYWQSPGDGGFPQEIIWKNSTNIKSLEILWPTPEQFEIL
                     GIQSVGYTNHVIFPLHLTLEDFSQPTLVVLDVTYLTCKDICIPGSAHLELFIPVGEKL
                     LTAHSHNIEKTLSQLPERNLQISFLKNIDIKSFANEKTVSFIAKVDAKEFFENPKVFL
                     HTDFGLPVIEPIISLSANAKHLEATFIFNKKLINAKQFDAQFIIADNNRSFLFTVPLE
                     VETSNVSKYPVPSFIIIIMTALLGGLILNGMPCVLPVLSIKLLSMLQHIEHPSSIRMS
                     FMVTSFGIVCSFGLL"
     gene            1135..1686
                     /locus_tag="EYO86_05015"
     CDS             1135..1686
                     /locus_tag="EYO86_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIC42425.1"
                     /translation="MNLTGQIIVSMPSLEDERFYKTVIYICAHSSEGSMGIIINKKID
                     YDLYPDLLQQLGIDKPLNNKKLFIRYGGPVESGRGFVLHSDDMVRKETLNIDKGVALT
                     STAEFFDDLSKGKGPKNCILALGYAGWQPGQLESEIMRNSWMSLSVDNSFLFDDEVSR
                     KWSQAYKLMGIDPNSLSFESGQA"
     gene            complement(1756..2595)
                     /locus_tag="EYO86_05020"
     CDS             complement(1756..2595)
                     /locus_tag="EYO86_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HIC42426.1"
                     /translation="MKIGFNLLLWTTNLGLKDFHLLEKLKKAGYDGVEVPIFEGSPEH
                     YHSISELLKNLDLECTGISVIPDEEHNPISSNKSNRNKAKDFLRWAVDCNQALNSKIM
                     CGPFHQPLAQFSGIPPTTIEKENGSEVHKEVANYAKKFDITLCIEPLNRFECYFLNTI
                     KDAYDYVKLVNEDNFKILYDTFHSNIEEKDPVGSISKFSDKIGHIHISENDRGVPGKG
                     NIPWSETFQAIQKINYKGWLTIEAFGRSLPDLAAATKVWRDFFSSEEEVYQEGINFIK
                     KNL"
BASE COUNT          938 a          439 c          437 g          915 t
ORIGIN      
        1 ctaataaacc aaaggagcaa acaatgccaa aggatgtaac cataaaactc atgcggatgg
       61 aggatgggtg ctcaatatgc tgaagcatcg ataatagttt tatggacaaa acagggagaa
      121 cacagggcat gccattgaga attaatcccc ccaacaaagc agtcataata attataataa
      181 aagagggcac tggatacttt gagacatttg atgtttctac ttctagagga acggtaaata
      241 aaaaacttcg attgttatca gcaataataa actgtgcatc aaactgtttg gcgtttatta
      301 attttttatt aaaaataaaa gtagcttcta aatgtttagc atttgcggat aaggaaataa
      361 ttggttcaat aactggtaat ccaaagtccg tatgtaaaaa aactttagga ttttcaaaaa
      421 actctttcgc atccaccttt gcaataaaag atacggtttt ttcatttgca aaagatttaa
      481 tatcaatatt tttgagaaaa ctaatttgca aattgcgctc cggtaattga gacaaagttt
      541 tttctatatt atgtgaatgc gctgtaagca atttttctcc cacaggaatg aataattcta
      601 aatgtgctga tcctggaatg caaatatctt tacaagttaa ataggtaaca tctaaaacaa
      661 ctaacgttgg ttgtgaaaaa tcttcaagtg taagatgtaa gggaaatata acatgattag
      721 tatatccaac ggactgaatg cctaaaattt caaattgctc gggtgttggc cataaaattt
      781 ccaatgattt aatgtttgta gaattcttcc atataatttc ctggggaaag cctccatcac
      841 caggagattg ccaatacgtt ttccatcctt tctgtaattg gtattccaaa cccacataaa
      901 tattttttga actatcattg tgggatatgg gtgaaattaa tcgtacctgt gattcgttac
      961 cgctgctcca atccgactgc aaagcccaaa gagatgtaaa ggaaaaaata acaataatga
     1021 aaaataaata tttaattaat cgcaacattg tcttaacttt tatttaattt gatataagta
     1081 ctatatatat gttaactatt gaaatcacaa aattgatgcg aatataaata caatatgaat
     1141 ttaactggtc aaattatagt ctctatgccc tctcttgaag atgagcgatt ttataaaaca
     1201 gttatttata tctgcgcgca ctcctctgag ggctcaatgg gcattattat taataaaaaa
     1261 atagattatg acttgtatcc tgatttactc caacaactag gtattgataa gcccctgaat
     1321 aataaaaaat tatttattcg ttatggtgga ccggttgaaa gcggaagggg gtttgtgctg
     1381 cattcggatg atatggtgag aaaagaaacg ctcaatattg acaaaggtgt cgcccttact
     1441 agtactgcag aattttttga tgacttgtcg aaaggcaaag gtccaaagaa ctgtatttta
     1501 gctttaggat acgcgggttg gcaaccggga cagttagaat ctgagattat gcgaaacagc
     1561 tggatgtcat tatccgttga taatagtttt ttatttgatg atgaagtatc cagaaaatgg
     1621 tcgcaagcct acaaactaat gggcattgat cctaatagct tgtcgttcga atcaggacaa
     1681 gcttaaattt ataattagaa aaaatattta aaatgagtga agtattttcc catcatttat
     1741 ataaaaacac ttactctaaa ggtttttttt aataaaatta attccttctt gataaacttc
     1801 ttcttcggag ctaaaaaaat cacgccaaac ttttgttgca gctgctaagt caggtaaaga
     1861 tcgtccaaaa gcttctatag tcaaccaccc tttgtaattt attttttgaa ttgcttggaa
     1921 agtttcactc catgggatat ttcctttacc aggaactcct ctatcatttt ctgaaatatg
     1981 tatatgaccg attttatctg aaaatttact aatagatcca actgggtctt tctcttcaat
     2041 attgctatga aaagtatcat ataaaatttt aaaattatct tcgtttacta atttaacata
     2101 atcataggca tctttgatgg tatttaaaaa ataacattca aatctattta aaggctcaat
     2161 acacaaagtg atatcaaatt tttttgcata attagcaacc tctttatgaa cttcactacc
     2221 attttctttt tcaatcgttg taggtggaat tcctgaaaat tgagcaagtg gttgatgaaa
     2281 tggaccacac ataattttgc tatttagtgc ttggttacaa tcaactgccc atctcaaaaa
     2341 atctttagct ttatttctat tcgatttatt tgaactaata ggattatgtt cttcatcagg
     2401 aataactgaa attcctgtac actctaaatc taaatttttt agtagttcag agatgctatg
     2461 atagtgctcg ggtgaccctt caaatatagg tacctcaact ccatcataac cggctttttt
     2521 taatttttct aataaatgaa aatcttttaa ccctaaattt gttgtccaaa gtaaaagatt
     2581 aaagcctatc ttcataataa aaaaattatt cgatacttat ccactgtgaa ttagaattac
     2641 ttgactgcac agtagcatta ataaatttca taatatgtaa accctcttca atatttggca
     2701 gaatttttat aagttcttta gcattacca
//
LOCUS       DTVV01000422            1056 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2274849, whole genome shotgun sequence.
ACCESSION   DTVV01000422 DTVV01000000
VERSION     DTVV01000422.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2274849"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="EYO86_05025"
     CDS             complement(<1..484)
                     /locus_tag="EYO86_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018997379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIC42427.1"
                     /translation="MLKTSSKKIYVKTYGCQMNVYDSNRIKNLFEPKGYTETASVEEA
                     DIVVLNTCHIREKAAEKVYSDIGRIAKKRNKNNSSKLTLVVAGCVAQAEGEEIKNRAP
                     LVDFVVGPQSYHTLPAMIEDNDKFINDEFLQNEKFKNLLFKSSDKVSEFVSVQEGCDK
                     F"
     gene            754..>1056
                     /locus_tag="EYO86_05030"
     CDS             754..>1056
                     /locus_tag="EYO86_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42428.1"
                     /translation="MFPGIKLDWPEGAALGFERILDARALAYRGPAFGWWTIPDQYSL
                     EVLHQREIMRPDRRPLFVTFASIMSHWPFGPTPPYQPDWSRVTGPAPFAPGALAKAL"
BASE COUNT          229 a          273 c          238 g          316 t
ORIGIN      
        1 aaaacttatc gcatccttcc tggacagata caaattcaga tactttatca gacgatttaa
       61 acagtaaatt tttaaacttt tcattttgta ggaattcatc attaataaat ttgtcattat
      121 cttcaatcat ggcgggtaaa gtatgatagg attgaggtcc aacaacaaaa tcaacaaggg
      181 gagcgcggtt cttaatttct tctccttcag cttgtgccac acatcctgcg acgactaatg
      241 ttaatttgct agagttattt ttatttcttt ttttagcaat gcgtccaata tctgaatata
      301 ctttttccgc agctttttct cttatatgac atgtgttcaa aaccacaata tcagcttctt
      361 caacacttgc tgtttctgtg tatcctttcg gttcaaataa attttttatt ctgtttgaat
      421 catagacatt catttgacat ccatatgttt taacatagat tttttttgaa gaagttttaa
      481 gcatttattt ttttttaaat aaacaagtat ctattttatg attacaaaaa ctataatgtt
      541 tttctttagc acgaattttt acgccggtcc ggctggcgca cggcatctgc cttcgtcgat
      601 tcgccgacct ttggcggcgc gtcctggctg gcccatgcga cgctgttatc cgggcgctgg
      661 atcacccgcg aggcggatta ccggctgttc ctggcggccc cctcggagac gctgggcgac
      721 cggttccgcg ccgccggcta ccggacaatc gggttgtttc cggggatcaa gctcgactgg
      781 ccggaaggtg cagcactggg gttcgagcgt atcttggatg cgcgcgcgct cgcctatcgg
      841 ggaccggcct tcggctggtg gacgatcccg gatcaataca gtcttgaagt cctccatcag
      901 cgcgaaatca tgcgcccgga ccgccggccg ctgttcgtga ccttcgcctc gatcatgagc
      961 cactggccgt tcggcccgac accgccctac cagcccgact ggtcgcgcgt taccggcccg
     1021 gccccgttcg cgcccggcgc gctggccaag gcgctg
//
LOCUS       DTVV01000423            2421 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2277830, whole genome shotgun sequence.
ACCESSION   DTVV01000423 DTVV01000000
VERSION     DTVV01000423.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2277830"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..375)
                     /locus_tag="EYO86_05035"
     CDS             complement(1..375)
                     /locus_tag="EYO86_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42429.1"
                     /translation="MNKKNDKDSLFCSFCGKSQKEVKKLIAGPTVFVCDECVELCMDI
                     IKEDSKNNKIKAKQNIPKPKEINSILNDYVIGQEKAKKTLSVAVYNHYKRLSNNTFSD
                     VEISKSTRAPSVAAVIIASGAG"
     gene            complement(375..986)
                     /locus_tag="EYO86_05040"
     CDS             complement(375..986)
                     /locus_tag="EYO86_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIC42430.1"
                     /translation="MKDPLENLTNNLIPMVVEQSSRGERAYDIYSRLLKERIIFLTGP
                     IDDNVASLVCAQLLFLESENPKKEISFYINSPGGIIWSGLAIYDTMQYISSKIMTICI
                     GQAASAASLLLTAGEKEIRFSLPNSRIMVHQPSGGYEGQVTDIEIQTKEIIKIKKRLN
                     EIYAKHTGKDITNVELIMERDKYFSPAEAIEFGLIDKIVEDRK"
     gene            complement(983..2335)
                     /locus_tag="EYO86_05045"
     CDS             complement(983..2335)
                     /locus_tag="EYO86_05045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:PRK01490.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIC42431.1"
                     /translation="MNIKEIDSKILFKEFEVQVPYKEVDNSINAKIAEIIPTVSLPGF
                     RKGKAPLNIVKKKYENNVLSEVIEKLVEDKTKKLLEEKKLKPFRQPKVEIKKYEKNEP
                     IEISIKIDLDPSIKIFPFSELELKKRSIELDQKTLKENYKNFISSQKHYHKVSDSRPA
                     KLSDKIIVNISTEDMSVPDFLKSQKNLPIITDSDYQVLPNISTKLISMNVKVGDKVNL
                     KFDLREILKEKNKKEVEFLIEILSLEYSHEFMITKEFLEKNNLKDKKELNDNLKNNLI
                     KQYEDQLKQIERKELMDILDVKNKFDVPAGMLNEEINSIWHRLEHAKKEGKLDEDDKN
                     VSDAALKRRYEKIALRRVKLAILMQHIANEEKILVTEKELTDGMLNYATQYPGQEKQI
                     LDYFKKNPPSVETIRGPIFEQKIVDHILSKVKLKNVTIDVKKFKKLQEETFINNKETG
                     "
     gene            complement(2346..2421)
                     /locus_tag="EYO86_05050"
     tRNA            complement(2346..2421)
                     /locus_tag="EYO86_05050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(2394..2396),aa:Leu,seq:tag)
BASE COUNT          699 a          416 c          307 g          999 t
ORIGIN      
        1 ctacccagcg ccagaggcaa ttatgacagc ggcaacggaa ggtgctctcg tagattttga
       61 aatttccaca tctgagaaag tattattgga taacctctta taatgattat atactgccac
      121 agataaagtt ttttttgctt tttcttgtcc gattacataa tcatttaaaa tagaattaat
      181 ttcttttggt tttggtatgt tttgtttagc ttttatttta ttatttttac tatcttcctt
      241 gataatgtcc atacaaagct caacacattc atcacaaaca aatactgttg ggcctgctat
      301 taattttttt acttctttct gacttttccc acaaaaagaa caaaaaagtg aatctttgtc
      361 atttttttta ttcattattt tctgtcctct acaattttgt caattaatcc aaattctatt
      421 gcctctgctg gagaaaaata tttatctctt tccattatga gctcaacgtt tgtaatatct
      481 ttgcctgtat gctttgcata gatttcattt aaccttttct taatcttaat tatttctttt
      541 gtttgtattt ctatatcagt gacttggcct tcataaccac cacttggttg atgaaccata
      601 atgcgtgaat taggtaaaga aaagcgtatt tctttctcac cagctgttaa caacaatgaa
      661 gcagctgaag cagcttgacc gatacatatt gtcataattt tagaggaaat atattgcata
      721 gtatcatata ttgccaaacc ggaccaaatt atacctcctg gagaatttat ataaaatgaa
      781 atttcttttt ttggattctc agattctaga aataatagtt gcgcacaaac taaacttgct
      841 acattgtcgt caattggtcc tgttagaaaa attattcttt ccttcagaag tctggaataa
      901 atgtcataag ctctctcgcc tcttgaagat tgctcaacca ccattggtat taaattattg
      961 gttaaatttt ctagagggtc cttcatcctg tttccttatt attaataaat gtttcttctt
     1021 gcaatttctt aaacttctta acatcaatag ttacattttt taacttaact tttgataaaa
     1081 tatgatcaac tattttttgc tcgaatattg ggccgcgaat agtctcaaca cttggtgggt
     1141 tttttttaaa ataatctaaa atttgctttt cttgaccagg atattgagta gcataattta
     1201 acatcccatc tgttaattct ttttccgtta ctaaaatttt ttcttcattt gctatatgtt
     1261 gcattaaaat cgctagcttc actcttctta gagcaatttt ctcatatctt cttttcaatg
     1321 cagcatcgga tacattttta tcatcttcat caagcttacc ttctttctta gcgtgctcta
     1381 acctatgcca aatagaatta atttcctcat ttagcatacc tgcgggcaca tcaaatttat
     1441 ttttaacatc taagatatcc attaattctt ttctctcaat ttgcttgagt tgatcttcat
     1501 attgttttat taaattgttt ttaagattgt catttagttc ttttttatcc tttaagttat
     1561 tcttttctag aaattcttta gtgatcataa actcatgact atattctaag gataaaattt
     1621 caataagaaa ctcaacttct tttttgtttt tttcttttaa tatctctctt aaatcaaact
     1681 ttaaattaac cttgtctcca accttaacat tcatactaat aagtttcgtg ctaatatttg
     1741 ggagtacttg gtaatctgaa tctgtaatta tgggtaaatt tttttgagat tttaaaaaat
     1801 ctggtactga catatcttcc gtcgaaatat ttactatgat tttgtcagac aatttagcag
     1861 gccttgaatc actaacttta tgataatgtt tttgtgacga aataaaattt ttataatttt
     1921 ctttcaatgt tttttgatct aattcaatag atcttttttt taattctaat tcactgaaag
     1981 gaaatatttt aattgaagga tctaaatcta tttttattga aatttctata ggttcatttt
     2041 tttcatattt cttaatttca acctttggct gcctgaatgg tttcagtttt ttttcttcta
     2101 ataatttttt agttttatcc tcaactaatt tttcaattac ctcagataag acattatttt
     2161 catatttctt ttttacaata ttgagagggg ctttgccttt acgaaaacca ggaagagata
     2221 cagtagggat gatttctgca attttagcat taattgaatt gtcaacttct ttatacggaa
     2281 cttgaacttc aaattcttta aacaatattt ttgaatcaat ttcttttata ttcatatgct
     2341 cctcatggta ggccggaaag gacttgaacc ttcacacctc gcggcacgag atcctaaatc
     2401 tcgcgtgtct accaattcca c
//
LOCUS       DTVV01000424            1304 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2281094, whole genome shotgun sequence.
ACCESSION   DTVV01000424 DTVV01000000
VERSION     DTVV01000424.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2281094"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="EYO86_05055"
     CDS             <1..220
                     /locus_tag="EYO86_05055"
                     /inference="COORDINATES: protein motif:HMM:PF00361.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42432.1"
                     /translation="LYKIRSGFYLFMFTIFGSILLIIGIIFLLLITGSTNLIVLENFH
                     FSVNQQKLFAFVFTIGFGIKVPIFPFHG"
     gene            260..370
                     /locus_tag="EYO86_05060"
     CDS             260..370
                     /locus_tag="EYO86_05060"
                     /inference="COORDINATES: protein motif:HMM:PF00361.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42433.1"
                     /translation="MLLAGILLKLGIYGLLRFGSTILPYGILYLSPLIFL"
     gene            506..772
                     /locus_tag="EYO86_05065"
     CDS             506..772
                     /locus_tag="EYO86_05065"
                     /inference="COORDINATES: protein motif:HMM:PF00361.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42434.1"
                     /translation="MGCIITSVNHGVSSSALFMFLGFLYDRTHSRNLFTLQALFHHYP
                     VSSTLFFILILGNLSLPGTLGFYGELFSILSLADVDFLLTALFV"
BASE COUNT          376 a          199 c          177 g          552 t
ORIGIN      
        1 attatataag atcagatctg gtttttatct atttatgttt acaatctttg gttccatatt
       61 attaataatt ggtataatat ttttattatt aattaccggg tctacaaacc taattgtctt
      121 agagaacttt cacttctctg taaaccaaca aaaactattc gcttttgtgt ttactattgg
      181 ttttggtata aaagttccta tatttccttt ccacggctga ttacccgatg ttcattctga
      241 aggttctact tatggttcta ttctactagc gggaatatta ttaaaattgg gaatctatgg
      301 attattaaga tttggttcaa ctatattacc ctatggtatt ctgtatttaa gcccacttat
      361 ttttttatga acacttgtag gatgctttac gtgttcttat aattgtttaa gacaatatga
      421 tctcaagaaa ataatagctt attcttctgt aacccatatc aattttgcta ttgcctctct
      481 atatacttta catattcaag gtttgatggg ctgtatcatc acatctgtta accatggtgt
      541 cagctccagc gccttgttca tgtttcttgg atttctgtat gacagaactc attctagaaa
      601 cctttttact ttacaagctt tatttcatca ctatccggta tcttcaaccc tcttttttat
      661 tttaatttta ggtaacttaa gcctaccagg tactttaggt ttctatggag aattgtttag
      721 tatattatca ctagcggatg ttgatttctt attaaccgct ttgtttgttt gaaatgtttg
      781 tttaacaacc tcttattctt tatttttata taaaagaatt tcctttggta aatcttttca
      841 tagaaaactt ataattggta ttcgtctcgg aaaacctttt tatctcaacc ctagactgtt
      901 gaatcttctt tcggggaagc aagatagatt aatatcttat cttttttgaa tgacttctca
      961 aaaaatttta aatatcgaat atgtatcaag tttgaaattt atttggtagc taaaatttat
     1021 aaggtcgtct cttactgtat cttcaataac ctttaaagat ttaaatagat tagattgttg
     1081 aatgagtttt attttcattt atttaattaa aatcttgggt ttaaaagggt atttattata
     1141 gttcgcataa ttgcattatt acccctttca aatttgctat ttgctttaac gattctatat
     1201 tttgaattag gaaaagcact ttgattatac aaaccatgta gtatgacgtt taattcgata
     1261 atcaacgtta attttaccaa tcctaatctt tagtaattgt aaat
//
LOCUS       DTVV01000425            1978 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2284300, whole genome shotgun sequence.
ACCESSION   DTVV01000425 DTVV01000000
VERSION     DTVV01000425.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2284300"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="EYO86_05070"
     CDS             complement(<1..1194)
                     /locus_tag="EYO86_05070"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIC42435.1"
                     /translation="MNTKNSIKDLGVRAKKAAGLLANISNDQKNKALNNLKKNLEFYS
                     NELIEINKKDIDNAHTMKLSVAMIDRLTLNQDRIKGMMKSLDDIMNLQNPVGKILSEW
                     ERPNGLHIKKISVPLGVIGIIYESRPNVTVDASAIAIKAGNAVLLRGGKDSFHSSQKL
                     NEIISKSFATAGLPDNSVQMVATSDRQAVDEMLRLDSYIDVIIPRGGKGLIKHVKEKS
                     SIPVIKHSDGICHIYVDKEADLNIAKKVIFNSKMRRPGICGATETVLIDKTLSENTSD
                     LLQNLIEAECEIRGDDYVKSLNTNFKKASEEDWGTEYLDKIISVKIVEGVQEAIHHIN
                     HYSSGHTDAIITQNSKTFQTFHQQINSAIILQNASTQFADGGEFGFGAEVGISTGKLH
                     PRGPVG"
     gene            complement(1191..>1978)
                     /gene="proB"
                     /locus_tag="EYO86_05075"
     CDS             complement(1191..>1978)
                     /gene="proB"
                     /locus_tag="EYO86_05075"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIC42436.1"
                     /translation="DSEMRRRYLNVRKTIYSLHKKNIIPIINENDTVATEEIRYGDND
                     RLAARVAQMIDADLLILLSDIDGLYSMSPNKNYKAKKIKDIYKIDTSIEKMADDQSSS
                     VGSGGMVTKIWAAKICMNSGCSTVITNSDKARPLININKDNSTWFHAVKTPRSSRKQW
                     LLNHLHSSGTIIIDEGAQKAVLNNKSLLPAGVIDIKGKFNRGDVISITNTKNIKIGIG
                     VIAYNAGDAKKIIGKNSKDIKNILGYEGRDELIHKDDLVKVKL"
BASE COUNT          552 a          360 c          316 g          750 t
ORIGIN      
        1 accaacagga ccgcggggat gaagtttacc agttgatata ccaacctcag caccaaaacc
       61 aaattctcca ccatctgcaa attgtgtaga tgcattttgt aaaatgattg cactgttaat
      121 ttgttggtgg aacgtttgaa atgttttaga attttgagtt atgatagcat ctgtatggcc
      181 agatgaataa tggttgatat gatgaatggc ctcttgcact ccctcaacta tttttaccga
      241 gataatttta tcaagatatt cagtacccca gtcttcttca gaggcctttt taaagtttgt
      301 gtttaaagat ttaacataat catcacccct aatttcacac tctgcctcta ttaagttctg
      361 caaaagatcc gatgtgtttt cagatagtgt tttatcgatt agtacagttt cagttgctcc
      421 acatattcca ggtcgtcgca ttttgctgtt aaaaattact tttttggcaa tgtttaaatc
      481 agcttcttta tctacatata tatggcaaat tccatctgaa tgtttaatga ctgggatgga
      541 acttttctct ttgacatgtt ttatcaatcc ttttcctcca cgaggaataa ttacatcaat
      601 gtaggaatca agcctgagca tttcatctac tgcttgtcga tcagacgttg ctaccatttg
      661 aactgagtta tctggcaatc ctgcagtagc aaaagattta ctaataatct cgtttagttt
      721 ttgcgaagaa tgaaaactat ccttacctcc tctgagaagg acagcgttac ccgctttaat
      781 ggcaattgct gaagcatcga ctgttacgtt gggtctagat tcatagatga ttccaatgac
      841 accaagggga acggatattt ttttaatatg caatccatta ggacgttccc attcagataa
      901 aattttgccg actggatttt gtaaattcat aatatcatcc aaacttttca tcattccttt
      961 aattctatct tgattaagag taagtctatc aatcattgca acggataatt tcattgtgtg
     1021 tgcattatca atatcttttt tgtttatttc aattaattca tttgaataaa attctaaatt
     1081 ttttttaaga ttattgaggg ctttattttt ttgatcatta gatatgttag ctaacaaacc
     1141 agctgctttt ttggctctca ctcctaagtc tttaattgaa tttttagtgt tcataatttt
     1201 actttaacca gatcatcttt gtgaattagt tcatcacgtc cttcatatcc aagtatgttt
     1261 ttaatgtctt tactattttt tccaataatt ttttttgcat cgcctgcatt atatgcaatc
     1321 accccaattc ctattttgat attttttgta ttagttatgc taatcacatc acctcggtta
     1381 aactttccct taatgtctat tacacctgct ggaagcagac ttttgttatt aagcactgct
     1441 ttttgagctc cttcatcaat aattatagtg ccagaagagt gtaaatgatt aagaagccat
     1501 tgttttcttg atgatcgcgg ggtttttact gcatgaaacc atgttgaatt atccttatta
     1561 atattaatta agggtctggc tttatcacta tttgtgatca ctgtagaaca tccagaattc
     1621 atacaaattt tagcagccca aatttttgtt accatacctc cgctacctac ggaagaagat
     1681 tgatcatcag ccattttttc aatggatgtg tctattttgt atatatcttt aatctttttg
     1741 gctttgtagt ttttattagg actcatggaa taaagtccat caatatcact gagtagaata
     1801 agtaagtcag catcgatcat ttgtgcgacg cgtgctgcta gtcggtcatt atcaccgtat
     1861 ctaatttctt cagtggctac tgtatcgttt tcattaataa taggaataat gtttttttta
     1921 tgaagagagt aaattgtttt cctaacgttg agataccggc gacgcatttc actatcct
//
LOCUS       DTVV01000426            1725 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2287461, whole genome shotgun sequence.
ACCESSION   DTVV01000426 DTVV01000000
VERSION     DTVV01000426.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2287461"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            130..1287
                     /locus_tag="EYO86_05080"
     CDS             130..1287
                     /locus_tag="EYO86_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIC42437.1"
                     /translation="MKIFFSILIFYSFLFIDAFADVKFSNYRDYKITNTNFQLEEIWK
                     GLNYPWGMTFIDEENLLITEKSGGLLRINVSTREQFNIFHDLNILASSQGGLLDVLYH
                     DNFVYFTYSHNHGERYSSTAIARGRLIDDTIKDTEILLIAEPKINQAKHFGSRIVIRD
                     NHLFASFGERGKGMIAQDPTTHPGSIIRINLDGSIPKDNPRFINQPTWLPEIYQIGVR
                     NPQGMTFSPFDSNIYISNHGAKGGDFVGIVNAGGNYGWKVIGWGGENYFGTKIGDGEP
                     FKEEYDKPLLSWVPSIAPSNIQFYNGNMFQDWKGDLLVSSLKFHMLVKLDMENSQIVN
                     EEIILRGCKMYKKPCHGDNTVGRIRDIEVDKKGRIYIITDEPESALWRISK"
     gene            complement(1388..1705)
                     /locus_tag="EYO86_05085"
     CDS             complement(1388..1705)
                     /locus_tag="EYO86_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIC42438.1"
                     /translation="MSIYINVGTYSNEGAGGLVEGDSDRRAAMETFHSSVGAKLLDYH
                     ITRGKYDFCVIVEADSYEMIAAMALMAKGSGTVNDLVTLESVDINEIRKIAKKVQFTA
                     PHK"
BASE COUNT          578 a          273 c          293 g          581 t
ORIGIN      
        1 aaacttagct atttgctttg ttaatctttt ttctgcttct tttttatact cttctttttt
       61 tcgctatctc tagcctgtca attactttct cttggtaata taaatataaa ttagtatata
      121 attattatca tgaaaatttt tttttcaata ttaattttct attcctttct atttattgat
      181 gcttttgctg atgtaaaatt tagtaactac agagattaca aaattacaaa tacaaatttt
      241 cagttggaag aaatatggaa aggtttaaat tatccctggg gcatgacttt cattgacgaa
      301 gaaaatcttt taatcactga aaaaagcgga ggattgttaa gaataaatgt ttctactaga
      361 gaacaattta atatttttca tgatttaaac atattagcct ctagtcaagg agggttatta
      421 gatgtgcttt atcacgataa cttcgtttat tttacctata gccataatca cggtgaaagg
      481 tacagcagta ccgctattgc aagaggaaga ctcattgatg atactataaa agatacagaa
      541 attttattaa ttgcagaacc taaaattaat caagccaaac attttggatc aagaattgta
      601 ataagagata atcatttatt tgctagtttt ggtgaacgag gaaaaggcat gatagcacag
      661 gatccaacaa ctcaccctgg aagtattatt cgtataaacc ttgacggatc tattcctaag
      721 gataatccaa gatttataaa tcaaccaact tggcttcctg aaatatatca aataggagtt
      781 cgcaatccgc aagggatgac cttttctcct tttgatagca atatatatat ttctaatcac
      841 ggagctaagg ggggtgattt tgttggtata gtcaacgccg gtggaaatta tggatggaaa
      901 gtaattggtt ggggaggaga aaattacttc ggtacaaaga taggtgacgg agaacccttt
      961 aaagaagaat atgataaacc tttattgagc tgggttccat caattgcgcc aagcaacatt
     1021 caattttaca atggaaatat gtttcaggat tggaaggggg atctgcttgt ttcatcttta
     1081 aaatttcata tgctggtaaa attagatatg gaaaactcac agattgttaa tgaggaaata
     1141 atcttgcggg gttgcaagat gtataaaaag ccttgtcacg gtgacaatac agttggcaga
     1201 atacgtgaca ttgaagtgga taaaaaagga cgtatttaca taataactga tgaaccagaa
     1261 tcagctcttt ggagaatttc taaataaaaa aaattatcta ttcttttcat ggattatcaa
     1321 aatttttcca tccaagttct taatttaatc taaaagagcg ccctacaaat gagggcgcta
     1381 ataatgatta cttgtgtggt gctgtaaatt gaactttttt tgcgattttg cgtatttcat
     1441 taatatcaac tgattctaag gtgactaaat cattaactgt cccactacct ttagccatta
     1501 aagccattgc agcaatcatt tcatatgaat cggcttcaac tatgacacag aaatcatatt
     1561 tgccacgagt aatgtgataa tctagtaatt tggctccaac actactgtga aaagtttcca
     1621 tagctgctct acgatcagaa tctccttcga caagacctcc agcaccttca ttagaataag
     1681 ttcctacatt tatatatata gacattaaac ttcctcctta aattt
//
LOCUS       DTVV01000427            1571 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2290377, whole genome shotgun sequence.
ACCESSION   DTVV01000427 DTVV01000000
VERSION     DTVV01000427.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2290377"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="EYO86_05090"
     CDS             complement(<1..782)
                     /locus_tag="EYO86_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020169638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC42439.1"
                     /translation="MKKNNLILYGSLLVIGLIAPFIFPAFKLQISFLYILIVLAMTWD
                     VQGGQMGYNTFGNILFFGIGMYFCASIQIGMFFPLAEWTASGGEKTFVHTPPQYFQGF
                     FLGLILAGIVPALVAALIGYGILGLRGHYFAICTLGLGIAAGEIAGGIELVGAGQGMT
                     TPPWPKGIGDTELRSEFFYYICFILMIATFIFLQWAYKTRFGLVLNAIRDNEDKAEAM
                     GIQTMKYKIIGWVVSAFFVGIAGGLMGNMVGYIEPTEVAFAGS"
     gene            complement(782..961)
                     /locus_tag="EYO86_05095"
     CDS             complement(782..961)
                     /locus_tag="EYO86_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42440.1"
                     /translation="MKKTVLKDDDGKSIEVDLKAFLDHLNEYHKTGTSTHTQSGCSFT
                     VDGEFRKKIRKLYEK"
     gene            complement(958..1515)
                     /locus_tag="EYO86_05100"
     CDS             complement(958..1515)
                     /locus_tag="EYO86_05100"
                     /inference="COORDINATES: protein motif:HMM:PF13181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxo-2,4-heptadienoate hydrolase"
                     /protein_id="HIC42441.1"
                     /translation="MNKIFLYTFLFFLFCGNAIADTKDREIKLDQLFEQLKKSSNASV
                     AFEIEMKIWNIWSTHPTQKELTQSLANGSDLMSQGKLEKSYKVFSTIIDVAPNWAEGW
                     NKRATVLYLMGRYQDSLNDIDEVLKRESRHFGALSGQGLVHIEMKNYEKAIKSYESVQ
                     KIYPSINSAKVMIPQLKILIKDEAI"
BASE COUNT          512 a          322 c          218 g          519 t
ORIGIN      
        1 gatccggcaa aagcaacttc agttggctca atataaccaa ccatatttcc catcaagcca
       61 cccgcaatac caacaaagaa agctgaaact acccagccaa taattttata cttcatcgtt
      121 tgaattccca tagcctcggc tttgtcttca ttatctctga tggcatttaa aactagtcca
      181 aatcttgttt tgtaagccca ctgtaagaaa ataaaagtcg ctatcatcaa aataaaacat
      241 atgtagtaga aaaattctga tctcagttca gtatcaccaa ttcctttagg ccacggtgga
      301 gttgtcattc cctgtccggc tccaactagc tcaataccgc ctgctatttc tccagccgcg
      361 attcccaaac ccagcgtgca tattgcaaag taatgtcctc ttaatcctaa aattccataa
      421 ccaattaatg cagcaacaag cgctggaact attccagcta aaatcagacc tagaaagaaa
      481 ccttgaaaat attgtggtgg ggtgtgcaca aaagttttct ctcccccgct tgcagtccac
      541 tctgcaagag gaaaaaacat tcctatttga atactggcac aaaaatacat tccaatacca
      601 aaaaagagaa tgttaccaaa agtgttataa cccatctgcc ctccctgaac atcccaagtc
      661 atagctaaaa caattagtat gtataaaaat gatatttgaa gtttaaaggc aggaaatata
      721 aatggcgcaa ttaaacctat aactaacaaa ctaccataga gaattaaatt attttttttc
      781 attatttttc atataatttt ctaatttttt ttctaaattc gccgtcaact gtaaatgagc
      841 atccactttg ggtatgtgtg ctcgttccag ttttatgata ttcgtttaga tgatcaagaa
      901 atgccttcag atcaacttca atggattttc catcatcgtc ttttaataca gtttttttca
      961 tatagcctcg tctttaatta atattttaag ttgaggaatc ataactttag ctgaatttat
     1021 agaagggtat attttttgaa cagattcata actttttatt gctttttcat aatttttcat
     1081 ttcaatatgt actagacctt gacctgacaa agcgccaaaa tgtctgcttt cacgtttaag
     1141 cacttcatct atatcgttta acgaatcctg gtaccttccc attaagtaaa gaactgttgc
     1201 acgtttattc catccttcag cccaattagg tgcaacatca ataattgttg aaaaaacttt
     1261 ataagatttt tctagtttac cttgagacat taaatctgaa ccgtttgcca aagactgggt
     1321 taattccttt tgagttggat gcgttgacca aatattccaa attttcatct ctatttcaaa
     1381 agctacggat gcattactac tttttttaag ttgttcgaaa agttgatcta acttgatttc
     1441 tctatcttta gtatctgcaa tcgcatttcc acaaaataaa aaaaacagaa acgtatatag
     1501 aaatatttta ttcattttaa gtattctctc ttcctagata gtgcaaatcg tctataaaca
     1561 agaattagaa c
//
LOCUS       DTVV01000428            1997 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2291388, whole genome shotgun sequence.
ACCESSION   DTVV01000428 DTVV01000000
VERSION     DTVV01000428.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2291388"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="EYO86_05105"
     CDS             <1..360
                     /locus_tag="EYO86_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIC42442.1"
                     /translation="NDLRNELMDNFGILKNGCAGVIKNATMEYKINGPLELIDKVLEI
                     GKSGIQINRYKGLGEMNPDQLWETTLDKNFRSLLLVKIDEVNETNTLFETLMGDVVEG
                     RRNFIQENSLKVVNLDI"
     gene            373..1626
                     /locus_tag="EYO86_05110"
     CDS             373..1626
                     /locus_tag="EYO86_05110"
                     /inference="COORDINATES: protein motif:HMM:NF033792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HIC42443.1"
                     /translation="MNYSIKLDNIPNNILLINLIKIRWLAIFGQLSAILISYYYLEIF
                     IPVFSCFLIIVVSAFVNLFSLFRKKINNYLSDNEAFYFLLFDTLQLGILLYLTGGIYN
                     PFSLLLIAPLIISASYLPIVFSVILSLLSIITVILISYFYIPITWNDSFTVPNVFRYG
                     LSLSLIISLIFIAIYVYLLANSSRNISQALFHTRSVLANQKKLSEIGSLSAAAVHEIS
                     TPLNTIFLILNDLRDEKFVRNNSEIKKEIELLKSQAEKCRAILLTLSRNPQNLKDKFF
                     DNTTLSNIIKLNFDKFNNRKIKLEINIIPKGNEPFVFFKDELMYGLGNIIQNAIQHAN
                     SIVKADIYWNEAQFKIIINDDGNGFPKEIFDKIGNPYISKNNNGMGLGIFIAKNLIEN
                     IGGSIFCKNEKDDGASVEVIIIRDT"
     gene            1632..1976
                     /locus_tag="EYO86_05115"
     CDS             1632..1976
                     /locus_tag="EYO86_05115"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIC42444.1"
                     /translation="MNRELLIVDDNVPFRIRLSKSMEKKGFVVESFSNSEQTKRRIAE
                     KKFDFAIVDMRLEDGSGLELIKLINSKHSNTKCLLLTGYGNIATAVAAIRFWFVLGPC
                     YVGNKVRCIIDT"
BASE COUNT          706 a          241 c          310 g          740 t
ORIGIN      
        1 aatgatttgc gaaatgaatt aatggataat tttggaattt tgaaaaatgg ttgtgcaggc
       61 gttattaaaa atgcaactat ggaatataaa attaatggac ctcttgaatt gattgataaa
      121 gttcttgaaa ttggaaaatc aggtattcaa attaatagat acaagggact aggggaaatg
      181 aacccagatc aattatggga aacaacactt gataaaaatt tccgctcttt gttattagtc
      241 aaaatagatg aagttaatga gacaaatact ctttttgaaa cattaatggg tgatgtggta
      301 gaaggccgga gaaattttat tcaagaaaat tcacttaagg ttgtaaactt agatatttag
      361 cagaatatct atatgaatta ctctataaaa ttagacaata ttccaaataa tattttatta
      421 ataaacttaa ttaaaattag atggttagct atttttggtc aactatcagc tattttaatt
      481 tcctattatt atctagaaat tttcataccg gttttttctt gtttcttaat tattgttgta
      541 tctgcgtttg ttaatttatt tagtcttttt agaaaaaaaa ttaataatta tttatcagat
      601 aatgaagctt tttatttttt gttatttgat acattgcaat tgggcattct tttgtatttg
      661 acaggaggaa tttacaatcc cttttctcta ttattaattg cccctttgat tatttctgca
      721 tcatatttgc caatagtctt ttcagttatt ctctctttat tgtctattat aacagttatc
      781 ttaattagtt atttttatat tccaataact tggaatgatt cttttaccgt tcctaatgtt
      841 ttcagatatg gcttaagttt atcattgata atttctttaa tatttattgc catttacgtt
      901 tatcttcttg cgaattcatc cagaaatatt tctcaagctc tttttcatac tagatcagtt
      961 cttgcaaatc aaaaaaaatt atctgaaata ggctctttga gtgcagctgc tgttcatgaa
     1021 ataagcactc cacttaacac tattttttta attttaaatg atttgcgtga cgaaaagttt
     1081 gtaaggaaca atagtgaaat aaagaaagaa attgaattat taaaatccca agcagaaaaa
     1141 tgcagggcca ttttactgac tctttcccga aatcctcaaa atttaaaaga taaatttttt
     1201 gataatacta ctctttcaaa cataattaaa ttaaactttg ataaatttaa taataggaaa
     1261 attaagctag aaattaacat tattccaaaa ggtaatgaac catttgtgtt tttcaaagat
     1321 gaattgatgt atggtttagg taatattatt caaaatgcta ttcaacacgc caattcaatt
     1381 gtcaaagcag atatatattg gaatgaagct caatttaaaa tcatcattaa tgacgatgga
     1441 aatggttttc caaaagaaat atttgacaaa attggaaatc cttatatctc aaaaaataat
     1501 aatggtatgg gcctgggtat ttttattgca aagaatttaa tagaaaatat tgggggttca
     1561 attttttgta aaaatgaaaa agacgatgga gcaagtgttg aagtaattat tatccgtgat
     1621 acttaaactt tatgaataga gaattattaa ttgttgatga caatgttcct tttagaatca
     1681 gattatcaaa atcgatggaa aaaaaaggat ttgtagttga atctttttca aattcagaac
     1741 aaaccaaaag aagaattgca gaaaaaaaat ttgatttcgc aatagttgat atgagattgg
     1801 aagatggttc gggtttagag cttattaaac ttattaattc taagcattct aatacaaaat
     1861 gccttttatt aaccgggtat ggaaatatag ccacagctgt tgcagctatt cgtttttggt
     1921 ttgtgcttgg cccttgttat gttggtaaca aggtgagatg cattatagat acgtgacaaa
     1981 tgtcaggtca attgaaa
//
LOCUS       DTVV01000429            1936 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2297825, whole genome shotgun sequence.
ACCESSION   DTVV01000429 DTVV01000000
VERSION     DTVV01000429.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2297825"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="EYO86_05120"
     CDS             complement(<1..314)
                     /locus_tag="EYO86_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_425111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42445.1"
                     /translation="MLKNYYLHKWRKNKNKLIQNHVYREEDEHSSCGVGLIASISGTP
                     SREVIEMGIQALKVLYHRGAVDADGKTGDGAGIQLDLPEEFFKEQIERAGHKTNDFPF
                     AVG"
     gene            complement(307..1764)
                     /locus_tag="EYO86_05125"
     CDS             complement(307..1764)
                     /locus_tag="EYO86_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC42446.1"
                     /translation="MLKFVDIQQRNPEKESVKARSKNFTEIYSRFVKKKAEEQSARCS
                     QCGIPFCQVHCPLQNNIPDWLKLTAEGRLEEAYEIASLTNNMPEVCGRICPQDRLCEG
                     NCVIEKSGHGAVTIGAVEKYITDVAWEKGWVKNIHVKKEKNISIGIIGSGPTGLAAAE
                     QLRIQGYQITIYDRYDRPGGLLIYGIPNFKLEKEIVIKRTQRLKNSGINFVMNCEIGS
                     SIKFSELQEKHDAILIATGVYKSRAINLNQYDAPNVIPALEFLIASNRKGLKDKVEDF
                     ENGKLNAKNKNVVVIGGGDTAMDCVRTAVRQGAKSVKCLYRRDQKNMPGSQHEVKNAI
                     EEGVDFHWLSLPVEFVGNERIKQTKIIKMQLGSADDSGRRKAEPINGSEEDLDTDLII
                     EALGFEPENLPIMFNKSELSVTQWGTLKINYKTMMTNIEGVFAAGDIVRGGSLVVWGI
                     KDGRDVAENIHNFLLKKERPLTYIVNENEKEFVHA"
BASE COUNT          576 a          391 c          250 g          719 t
ORIGIN      
        1 cctactgcga aaggaaaatc atttgtttta tgtcccgctc tctcaatctg ctctttaaaa
       61 aattcttcag gaagatctag ctgaattccg gctccatcac ccgttttgcc atcagcatca
      121 acagctcctc ggtgataaag aacttttaaa gcttgaatgc ccatttcaat aacttctctt
      181 gaaggagttc cgctaataga tgcaattaat cctactccac aagatgaatg ttcatcttct
      241 tcacggtaaa cgtgattttg aattaactta tttttatttt ttctccattt gtgtaaataa
      301 taatttttaa gcatgaacaa attccttttc attctcattg actatgtaag ttagaggcct
      361 ttcttttttt aacaaaaaat tatgaatatt ttccgcgaca tcacgaccat cttttattcc
      421 ccagacaact agactaccac cccgaacaat atcccctgca gcaaagacgc cttctatatt
      481 ggtcatcatc gttttgtaat ttattttaag tgtcccccac tgtgtcacag aaagttcact
      541 tttattaaac ataataggta agttttctgg ttcaaaaccc aaagcttcaa taattaaatc
      601 agtatctaaa tcttcttctg aaccatttat tggttcagct ttacgtctac ccgaatcatc
      661 agctgatcca agttgcattt taataatttt tgtttgttta attctttcat tgccaacaaa
      721 ctcaacagga agggacagcc aatgaaaatc aactccctct tcaattgcat ttttaacttc
      781 atgttgagaa ccaggcatat ttttttgatc tcgacgatat aaacatttaa ctgattttgc
      841 accttgccga acagccgttc ttacgcagtc catcgcagta tctccgcctc caataacaac
      901 tacgttttta ttttttgcat ttaactttcc attttcaaaa tcttcaactt tatcctttaa
      961 gccttttcta ttacttgcaa ttaaaaactc taaagctggg ataacattgg gagcatcata
     1021 ttgatttaga tttattgctc tagatttata cactcctgtt gcaatcaaaa ttgcatcatg
     1081 tttttcttga agttcactaa attttataga agatcctatt tcacaattca tcacaaaatt
     1141 gattccacta ttttttaatc tctgagttct tttaattact atttcctttt ctaatttgaa
     1201 attagggatg ccataaataa gaagtccgcc aggtctatca taacgatcat aaattgtaat
     1261 ttgataaccc tgaattcgga gttgttccgc tgcagctaaa cctgttgggc cagatccaat
     1321 aataccaatt gaaatattct tctctttctt gacatgtata ttttttaccc atcctttttc
     1381 ccaggctaca tcagttatat atttttcaac agcgcctatt gtaactgctc catgacctga
     1441 tttttcaata acacaatttc cctcacacaa tctatcctga ggacaaatac gtccacacac
     1501 ctcgggcatg ttattcgtta aacttgctat ttcatatgcc tcttcaagcc gtccttcagc
     1561 agttagcttt aaccaatctg ggatattatt ttgaaggggg caatgaactt gacaaaaggg
     1621 aataccgcat tgagagcatc gggcagattg ttcctcagcc ttttttttta caaatctaga
     1681 atatatttca gtaaaatttt ttgagcgtgc ttttacagat tctttttctg gatttctctg
     1741 ttgtatgtca acaaatttta acatttagtc ctatttttgt actaatattc aattaatttt
     1801 attcattata gacggtatta agaaaaacaa ataaataatg cttaaaatag tattaatatt
     1861 gtactaatta taaaaaaatc tatattttat aggagaaatt agttgttttt tttaataatt
     1921 tttttaattt tttcac
//
LOCUS       DTVV01000430            1264 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2300704, whole genome shotgun sequence.
ACCESSION   DTVV01000430 DTVV01000000
VERSION     DTVV01000430.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2300704"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1264
                     /locus_tag="EYO86_05130"
     CDS             <1..>1264
                     /locus_tag="EYO86_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIC42447.1"
                     /translation="DDLNSKTLPSQWRAKPVAYFEQTEDEIETIPSVLEAKIGGKRNE
                     QVDVIIDTLALEGYNINIENLINTVNQNNMMVSAGEQDTGDGSFNIKVPGLYETIDDV
                     LDTPIKTFGDSVITFRDIAKVKRTFEDRKSYANVNGKKAVTIEVSKRVGENIIDTIKK
                     IKEVSEQVTKDFPPSVDISFSQDQSKTIQTMLNSLQNNVIAAIILVLIIILGALGVRS
                     GFLVGVSIPGSFLSGILLLSIMGFTMNIVVLFALILSVGLLVDGAIVIVEYADRKIKE
                     GLTIKESFAGASKKMARPIISSTATTLAAFVPLLFWPGLAGEFMKYLPITLICVLTSS
                     LFMALLFVPVLGTIINTITRVFLQFVIPTLLSLILFNIFSILTNYVDINGLKIPLTII
                     KYLASLALFIFTFIKIIPSVYKISETINK"
BASE COUNT          445 a          181 c          203 g          435 t
ORIGIN      
        1 gacgatttaa attctaaaac tttaccaagt caatggcgcg ctaaacccgt tgcttacttt
       61 gaacaaacag aagatgagat tgaaacaatt ccaagtgttc tagaagctaa aattggtggg
      121 aaacgtaatg aacaagttga tgtgattata gatactttag ctttggaggg atacaacatt
      181 aacattgaaa acttaatcaa tactgtcaat caaaataata tgatggtctc tgctggagag
      241 caagatactg gggatggcag ctttaatata aaagtaccgg gattatatga aacaattgat
      301 gatgtattag atacgccaat taaaacattc ggtgattctg tcataacttt tagagatata
      361 gctaaagtta aaagaacttt tgaagatagg aaatcttatg caaatgttaa tggaaaaaaa
      421 gcagtcacta ttgaagtttc aaaaagagtt ggggaaaata ttattgatac aatcaaaaaa
      481 attaaagagg taagtgagca agtaacaaaa gattttcccc ctagtgttga tatatctttt
      541 agccaagacc aatctaaaac aattcaaaca atgcttaata gtcttcaaaa caatgtaata
      601 gctgcaatta tattagtttt aataatcatt cttggggctc taggagtaag atcaggattt
      661 ttagttggtg tttctatacc aggttctttt ttgtcaggca tattacttct ttccataatg
      721 ggatttacta tgaatatagt tgttttattt gcacttattt tatctgttgg attgcttgta
      781 gatggagcaa tagtaattgt tgaatatgcg gatagaaaaa taaaagaagg tttaaccatt
      841 aaggaatctt ttgcaggtgc atctaagaaa atggcgcgtc ctataatttc ttctacagca
      901 acaacgctag cagcctttgt tccccttctt ttttggcctg gattagctgg tgaatttatg
      961 aaataccttc ctataacgtt aatatgcgta ctaacatctt cattatttat ggctctttta
     1021 tttgttccag tacttggaac aataatcaat accattacaa gagtatttct tcaatttgtt
     1081 ataccaactc tactttcttt aattctattt aatatttttt caatattaac aaattatgtt
     1141 gatattaatg gtttaaaaat accacttaca ataattaaat atttagcatc attagctctc
     1201 tttattttta ctttcataaa aattatacca tcagtttaca aaatcagtga aacaataaat
     1261 aaaa
//
LOCUS       DTVV01000431            2179 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2301582, whole genome shotgun sequence.
ACCESSION   DTVV01000431 DTVV01000000
VERSION     DTVV01000431.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2301582"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="EYO86_05135"
     CDS             <1..826
                     /locus_tag="EYO86_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC42448.1"
                     /translation="YRFAAAESVSKQHIPTTISIILLSGLVSAVIGPNIATISKNIIT
                     DHLYAGSYLFLSLLTFIPFCLFIFFNNEKNDLEKKVIQGRSYLTLLSQPRFFQAVVSA
                     AIGYVIMSFLMTATPIEMHVIQQMSLNKTGFVIQMHIFGMFLPSLFTGILIKKFGHSK
                     IMYVGILILIICVIINFSHQHFYNYLAGLILLGIGWNFLFISGTSLLIISYKPEEKFK
                     AQGLNDFLVFSTQAVGALAAGYLLYLTNWQFINILCLPLLIIILLATLYADKITNN"
     gene            841..>2179
                     /locus_tag="EYO86_05140"
     CDS             841..>2179
                     /locus_tag="EYO86_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020027725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC42449.1"
                     /translation="MLDKRKFYINGKWENPLASKDFDVINPSNEEVFAIISLGDITDT
                     NAAVEAAKKSFVSWSKTPKEKKITLLENLYEVYKSRWNEMSEAISLEMGAPIDWATEE
                     QSSSGADHIKSFIQTLKNFEFEKSFSEDSDNHITYEPIGICALITPWNWPINQITLKV
                     IPALSAGCTMILKPSEIAPISGMVFAEMIHEAGFPPGVFNLVNGDGAGVGTQLSSHKD
                     IDMISFTGSTRAGRLITKNSADTIKRVCLELGGKGGNIVFADSDSDAVKRGVKHVFGN
                     SGQSCNHPTRMLVEKPIYERAVKEATEEAKSTKVNISSQKGDHIGPVVSKIQYDKIQK
                     LIQSGIDEGATLVAGGLNRPEGLEKGYFVRPTVFADVNNNMKIAKEEIFGPVLSILPF
                     ETEEEAIAITNNTSYGLTNYVQTLDKEKAKRVARQLRSGGVEINGKGASSGTPF"
BASE COUNT          733 a          349 c          384 g          713 t
ORIGIN      
        1 atatcgtttt gcagcagcag aaagcgtttc gaaacaacac ataccgacaa ctatctccat
       61 tatattatta tcaggcttag tttcagctgt tattgggcct aatatagcga ctataagcaa
      121 aaacataata acagaccatt tatacgcagg ttcatattta ttcttgtctt tattaacctt
      181 tatacctttt tgtttattta ttttttttaa caatgaaaaa aatgatttag aaaaaaaagt
      241 tattcaagga cgtagctatt taacattatt atcccaacct cgttttttcc aagctgttgt
      301 ttcagcagca attggttatg tgataatgtc tttcttaatg actgctactc caatagaaat
      361 gcatgtcatt caacaaatga gtttgaacaa gacaggcttt gtaattcaaa tgcatatttt
      421 tggcatgttt ttaccctcat tatttacagg aattttgatt aaaaaatttg gacatagtaa
      481 aattatgtat gtagggattt tgattttaat catttgtgtt atcattaatt tttcacatca
      541 acatttttac aattatcttg ctggactaat tttactaggc attggttgga attttctttt
      601 tatttctgga actagtttat taattatttc ttataaacct gaagaaaaat ttaaagccca
      661 aggattaaac gatttcttgg tattttcgac tcaagccgtt ggggctttag ctgccggtta
      721 tttattgtat ctgacaaatt ggcaatttat caatatttta tgcttacctt tattaattat
      781 tatattgctt gcaactttgt atgcagataa aattactaat aattaatttt aaaaatatat
      841 atgttagata aaagaaaatt ttatattaat ggcaaatggg aaaatccact ggcttctaag
      901 gattttgatg tcatcaaccc atccaacgaa gaagtttttg caattatttc acttggagat
      961 atcactgaca caaatgcggc agtggaagct gctaaaaaat cttttgtttc gtggtcaaaa
     1021 accccaaaag aaaaaaaaat cactcttctt gagaatttat atgaagttta caaatctaga
     1081 tggaatgaaa tgtcagaagc tatttcctta gaaatgggtg ctccaattga ttgggcaaca
     1141 gaagaacaaa gttcttccgg tgcagatcac attaaatctt ttatacaaac attaaaaaat
     1201 tttgaatttg aaaaatcttt tagcgaagat tctgataacc acattaccta tgaaccaatc
     1261 gggatatgtg ctttaattac accatggaat tggccaatca atcaaataac tttaaaagtt
     1321 atcccagctc tttcagctgg ctgcacaatg attttaaaac cttctgagat tgcacccatc
     1381 tcgggtatgg tatttgcaga aatgatacat gaggcaggat ttcctccagg tgtttttaac
     1441 ttagttaatg gtgacggcgc aggcgtgggt acgcaattat cctcacacaa agatattgat
     1501 atgatttcat ttacaggttc aaccagggca ggaaggctca ttaccaaaaa ttcagcggat
     1561 acgataaaaa gagtttgttt agaattggga ggtaaaggag gaaatatagt gtttgctgat
     1621 tcagactcag atgcagttaa aaggggtgta aaacatgtat ttggtaattc gggtcaatcg
     1681 tgtaaccatc caacacgcat gcttgttgaa aaacctattt atgaaagggc tgttaaagaa
     1741 gctacagaag aagccaaaag cacaaaagtt aacatttcat cacaaaaagg ggaccatatt
     1801 ggccccgtag tatcaaaaat tcaatatgat aaaattcaaa aattaattca aagcggtatt
     1861 gatgaaggag cgactttagt tgcaggcgga ctcaatcgcc ctgaaggttt agaaaagggg
     1921 tattttgttc gccccacagt ttttgctgat gtaaataata acatgaaaat tgccaaagaa
     1981 gagatttttg gccctgtatt atcaattttg ccttttgaga ctgaagaaga ggccatagct
     2041 attaccaaca atacttctta tggattaact aattacgttc aaacactgga caaagaaaaa
     2101 gccaaacgtg ttgcaagaca attacgctca gggggagttg aaattaatgg taagggcgcc
     2161 tcttcaggaa ccccatttg
//
LOCUS       DTVV01000432            3433 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2308856, whole genome shotgun sequence.
ACCESSION   DTVV01000432 DTVV01000000
VERSION     DTVV01000432.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2308856"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            22..1587
                     /locus_tag="EYO86_05145"
     CDS             22..1587
                     /locus_tag="EYO86_05145"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIC42450.1"
                     /translation="MNNIKEPSSKNKGLGSLFNIKTINISKSFGGTKALDDINLELMA
                     GEIHGLVGQNGAGKSTLGKIIGGYLQSSEGKLILNNKEIEKWSPKTALDNGVAMIHQE
                     LALVPLMSVFENIFLGIEENKFGVLEKKNLDFFYQLEEKISFGLNPNDIVGNLRIADQ
                     QKVEIMRALARNAKIIIMDEPTSSLTKDEINRLHALMLQLKSSKYLIIYISHFLDAIL
                     EVCDKVTILRDGKLIRTSAIKNENKEMLVEAMLGQPATITFPKKEFSSIEDHKTIFEI
                     DKLKTDTGLMNVSLKVNYGEIVGLLGLVGSGRIETLRAIFGADKILHGKMRYLEKNLK
                     IKNPNDAIKKGIVMIPEDRRKLGLVFTQNTKSNISITNLNKISNLEILNGLKEKTLVQ
                     KLINQVDIKPNVIDGNISYYSGGNQQKVLFAKWMFNSPNLILLDEPTRGVDVGAKRKI
                     YELIRELASKGIGMILVSSELEEVMGLADRAYLIKNGKTYDEIIPQDSSLDKVLFSLF
                     GAKKNNMNNHAVS"
     gene            1574..2503
                     /locus_tag="EYO86_05150"
     CDS             1574..2503
                     /locus_tag="EYO86_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC42451.1"
                     /translation="MQLANIINFSRNYAVLILIVLLMIILSFASEAFLTPRNLLNILN
                     QNAPLAIIASALTLVIIVGGFDLSTAGIFGVATVSAAWIAVNINPFLGLAVTPFIGII
                     MGYINGVLITSLKVHSFLATIATSLVFRGIAILITGGFLISVRMKEFTWLGRDKLFTV
                     HYSVYILIVFALLTTFILNKTTIGRYIYAVGGNEDAAILSGIKANFTKIFAFTFCGLA
                     CGIAAAIQVSRISMGAPQAGLGMELQAIAAVILGGTSIYGGSGAVWRSVSGVMLLALI
                     TNGFNILNAEPFYKDLTTGVVIVAAVALTAGRK"
     gene            complement(2507..3366)
                     /locus_tag="EYO86_05155"
                     /pseudo
     CDS             complement(2507..3366)
                     /locus_tag="EYO86_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1192 a          514 c          563 g         1164 t
ORIGIN      
        1 tatttaagaa aaaaaaatta aatgaataat ataaaggagc ctagttctaa aaataaagga
       61 cttggctcct tatttaatat taaaacgatt aacatatcaa aatcatttgg tggcactaaa
      121 gcactagatg atatcaatct ggaattaatg gctggcgaaa tacatggatt ggttggtcaa
      181 aatggggctg gaaaatcaac tttaggaaaa attattggtg gctatcttca atcttctgag
      241 ggaaaattaa ttcttaataa caaagaaatt gaaaaatggt ctcctaaaac tgcccttgat
      301 aatggcgttg caatgattca tcaagaactt gcactagttc ctctaatgtc tgtatttgaa
      361 aatatttttc ttggcattga agaaaataaa tttggtgttc ttgaaaaaaa aaatttagat
      421 tttttttatc aacttgaaga aaaaattagt tttggattaa accccaatga tatcgtgggt
      481 aatttacgta tagcagatca acaaaaagtt gaaattatgc gagccttagc acggaatgcg
      541 aaaatcataa taatggatga accaacatct tcacttacga aagatgaaat aaatcgacta
      601 cacgctttga tgttacaatt aaagtcatct aaatatttaa ttatttatat ttctcatttc
      661 ttagatgcca tcctagaagt gtgcgataaa gttacaatct taagagatgg caaacttatt
      721 agaacaagtg cgataaaaaa tgaaaacaaa gaaatgttag ttgaagcaat gcttggtcaa
      781 ccagccacaa taacatttcc aaagaaagaa ttttcttcaa ttgaagatca taaaacaatt
      841 tttgaaattg ataaattaaa aacagataca ggacttatga acgtatcatt aaaagtaaac
      901 tatggtgaaa ttgttggttt gcttggactt gttggaagtg ggagaataga gacactaaga
      961 gcaatatttg gagcggataa aattttacac ggcaaaatga gatatctaga aaagaattta
     1021 aaaataaaaa atcccaacga tgctattaaa aaaggtattg tgatgattcc tgaggatagg
     1081 agaaaattag gattagtttt tactcaaaat acaaaatcta atatatctat tacaaactta
     1141 aataagatta gtaatcttga aatcttaaac ggacttaaag aaaaaactct tgttcaaaaa
     1201 ttaattaatc aggtagatat taaaccaaat gttatcgatg gtaacatatc ttactactcc
     1261 gggggtaacc aacaaaaagt attatttgcg aaatggatgt ttaattctcc aaatttaatt
     1321 ttacttgatg aaccaactcg cggagttgat gttggggcaa aaagaaaaat ttatgagttg
     1381 attagagagt tagcatccaa gggaattggt atgatactag tttcttctga acttgaagaa
     1441 gtaatgggat tggctgatag agcttattta atcaaaaatg gaaaaactta tgatgaaatt
     1501 attcctcaag attcttcatt ggataaagtt ttgttttcac tatttggtgc aaaaaagaat
     1561 aacatgaaca atcatgcagt tagctaatat aataaatttt tcaagaaatt atgcggtttt
     1621 aattttgatc gtattactaa tgattattct ttcctttgct agtgaagcct ttttaacacc
     1681 tagaaactta ttaaatattt taaatcaaaa tgccccattg gcaataattg caagcgctct
     1741 aacattggta attattgttg gaggatttga tctttcgact gcaggaatat ttggtgtggc
     1801 cactgtttcg gcagcctgga ttgctgtaaa catcaatcct tttttaggtt tggctgtaac
     1861 tccatttatt ggaattatta tgggttatat aaatggagtt ttaattactt cattaaaagt
     1921 ccattctttt ttagctacta tagcaacaag tctagttttc agaggcattg caattttaat
     1981 tactggtggc tttttaattt ccgtacgcat gaaagaattt acgtggcttg gaagagataa
     2041 actgtttacg gttcattatt cggtatacat tctaattgtt tttgcactgt taactacctt
     2101 tatactcaat aaaacaacaa ttggacgcta tatttatgct gtaggtggca atgaagatgc
     2161 tgctatatta tctgggatta aggctaattt tacaaaaatt tttgcattta ctttttgtgg
     2221 tttagcttgt ggaattgcag cagccattca agtatcaagg atatccatgg gtgccccaca
     2281 agctggccta ggaatggaac tgcaagcaat agctgcagta attttaggtg gaactagtat
     2341 atatggagga tctggagctg tatggcgttc tgtttctggc gtaatgcttt tggctttaat
     2401 aactaacggt tttaacatac ttaatgctga accattttat aaagacttaa caaccggcgt
     2461 tgtaattgta gcagccgttg cattaactgc tggaagaaaa taataattat ttttttatta
     2521 gctcactaat aaataaatca ccgtatcctt tttcttttgc ttcttgataa aaatattttg
     2581 taactgaaga taatttctca gcatggggca tatcttgaaa aaattcatta atatatgtta
     2641 aatcttttaa ggcgttatta acgctaaaca tgtatctctt ataatcatta ttgatagctt
     2701 tttcggccat acgatttaag gcccctgaat ttccagagcc aagttttgct acatcataaa
     2761 gtttctttaa gtcaattcca agtttatcag cagctttaag tgtttcgata acaaaagttg
     2821 ctgttccgag tgaaagaaaa ttattaacca attttgcttt tgcacccatg ccaacaggac
     2881 caaagtaaaa aacatcctca caaaaattca ttaaaatttc ttttgcaaat tcaaaatcct
     2941 tctcttcaca accaactatt gccctaaaac tccctcttca gcctgaacag gtcctcccat
     3001 tacaggcgat tcaacataca taatatctaa agaatttaat ttttaaaaaa tttaattgaa
     3061 ccgtctgctt tatgtgttgt tatgtcaatt atcatagttt ttttttgtaa atgaggagcg
     3121 atctcactaa ttatatctac agctactgga gtattggtaa cgcacattat aataacgtta
     3181 gacaaacttg ctaattcctc taaattagtt gcttcttttg cacctttttt gaactaaatc
     3241 atcaataggt tttctatttt tatgagcaaa aactgtgaga ttataattat tgaccaaatt
     3301 aagtgcaatg ccgtaaccca tataaccagc accaataaaa ccaattttta ttttaccttt
     3361 attcatttgt ttgttcataa gctaactaat ttctgatact ggttcaccta atacatcata
     3421 gttaggctca aca
//
LOCUS       DTVV01000433            1275 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2314579, whole genome shotgun sequence.
ACCESSION   DTVV01000433 DTVV01000000
VERSION     DTVV01000433.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2314579"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="EYO86_05160"
     CDS             complement(<1..914)
                     /locus_tag="EYO86_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIC42452.1"
                     /translation="MSNQKINKLISILDNQDLDALALVPGSNFRYATGGNFHLMERPT
                     LLIVSKTCRPIAILPVLEVESFAQLNIDADIVEWQDSDGYQSAFDKGFNKLGSISKIG
                     VEGQRMRVFEMQAIEKSLPNITIVNAQKLISKMRLIKDHQEIEHLRQAVKIAESALTD
                     TIKFVTVNKTEIEIKNFLIQQLYQHGAEDIAFDPIVLAAENSALPHGHSRNDYHISED
                     DCLLFDFGATVNGYHSDITRTFFVGSASDEYKNIYAAVLKANQIGKKISKPKLTLHDL
                     DDQVLNSLCDSGYEDLIVHKTGHGLGMDV"
     gene            complement(941..>1275)
                     /locus_tag="EYO86_05165"
     CDS             complement(941..>1275)
                     /locus_tag="EYO86_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIC42453.1"
                     /translation="NILPVAYCPLAKGELLDNKMILEIANKYSKTPAQIVLRWLNHQN
                     WIAIPKSANENRMKENMEIFDFTISPDDINSLHSLARNFRAVPCALGTNHQIDLPSKH
                     GVNGLELI"
BASE COUNT          404 a          241 c          179 g          451 t
ORIGIN      
        1 acatccatac ccaatccatg tccggtctta tgtacaatta aatcttcata accggaatca
       61 catagagaat ttaaaacttg atcatcaaga tcatgcaaag ttaatttagg ttttgaaatc
      121 tttttcccaa tctggtttgc tttcaaaact gctgcataaa tgtttttata ttcatctgat
      181 gctgatccaa caaaaaaagt ccttgtgata tcggaatgat aaccatttac agttgcacca
      241 aaatcaaaga gcaaacaatc atcttcgcta atatgataat catttcttga atggccatga
      301 ggtaaagctg aattctcagc agctaaaaca atgggatcaa aagcaatatc ctccgcaccg
      361 tgttgataaa gctgttgtat tagaaagttt ttaatttcaa tttccgtttt atttaccgta
      421 acaaatttta tcgtatcagt tagagcagat tcagctattt taacagcctg tcttagatgt
      481 tcaatttctt gatgatcttt tataagtctc attttgctga taagtttttg cgcattgaca
      541 attgttatat ttggtagaga tttctctata gcctgcattt caaaaactct catacgctgt
      601 ccctccactc cgatttttga aatagagcct aatttattaa aacctttatc aaaagctgac
      661 tgatacccat cactatcttg ccattcaaca atatcggcat ctatatttaa ttgagcaaaa
      721 ctctccactt ccaacacagg taatatagca ataggcctgc atgtttttga aacaattaac
      781 aaagttggcc tttccattag atggaaatta ccccctgttg catatctaaa gttcgagcct
      841 ggaaccaaag ctagtgcatc taaatcttga ttatctaaaa ttgaaattag tttgttaatt
      901 ttttgattac tcatatttta tattcagcac aattattttt ttatatcaac tccaaaccgt
      961 taacaccatg cttggaaggc aaatcaattt gatgattggt gcctaatgca caaggaactg
     1021 ccctaaaatt tctagctaga ctatgtaatg aatttatatc atctgggcta attgtaaaat
     1081 caaatatttc catattttct ttcatcctat tttcatttgc tgattttgga attgcaatcc
     1141 aattttgatg gttcaaccat cttaaaacga tttgtgccgg agttttcgag tatttgtttg
     1201 ctatttctaa aatcatttta ttatccaaca attcaccttt tgccaaagga caatacgcaa
     1261 ctggaagaat attat
//
LOCUS       DTVV01000434            1060 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2317108, whole genome shotgun sequence.
ACCESSION   DTVV01000434 DTVV01000000
VERSION     DTVV01000434.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2317108"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            175..>1060
                     /locus_tag="EYO86_05170"
     CDS             175..>1060
                     /locus_tag="EYO86_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIC42454.1"
                     /translation="MGQVIKLYAKKNSFNNQVSELINLCQGDLETINSIIIEKLGSKV
                     PLIHEIASYLILSGGKRLRPLLTSCSFRLFEKNTNENINHIGLAAAVEFIHAATLLHD
                     DVIDESKKRRGNLSVNEVWGNKTSLLVGDFLFSRAFQLMAKYGNTAVLKLLSDTSVII
                     SEGEMLELSNDKDPTINEDVYYDVINGKTASLFSAACQVGGMSAQGSDKEVEYLKSFG
                     ANFGMSFQLIDDAIDYSSSNIVLGKNIGDDFKEGKVTLPIILAYLRSNKAEKEFWKKT
                     IVHLKQEKDDFHHAINIIK"
BASE COUNT          389 a          139 c          182 g          350 t
ORIGIN      
        1 agatctacta aataaggatt ttaacatatg ttctcatatc aaaagttata ggtaggagaa
       61 agttaattat ttaagtatat ttacaacaaa tttgagtgtt tttttacata aaaaaactta
      121 tatcagcatt atattatatg atgaaaaaat taaaattaag cttagattaa taatatgggt
      181 caagttatta aattatatgc taaaaaaaat agcttcaata accaagtttc tgaacttatt
      241 aatttatgcc aaggggatct tgaaacaatc aatagcatta tcatagaaaa acttggtagc
      301 aaggttcctc tcattcatga aattgcatca tatcttattt tatctggtgg taaaagacta
      361 cggcctctct taacatcttg cagttttcgt ctttttgaaa aaaatactaa cgaaaatatt
      421 aatcatattg gacttgctgc tgctgtagaa ttcattcatg ctgcaacttt actgcatgat
      481 gatgtcattg atgagagcaa aaaaagaaga ggtaatttat cagtcaatga agtatggggg
      541 aataaaacaa gtttactagt tggagatttt ttatttagcc gtgccttcca actgatggca
      601 aaatatggga atactgctgt tttaaaatta ttgtcagata caagtgttat tatatctgaa
      661 ggagaaatgt tagaattaag taacgacaaa gatcctacga ttaatgaaga tgtttattat
      721 gatgttatca atggcaaaac agcctctctc tttagtgcag cttgtcaagt tggggggatg
      781 agtgctcaag gttcagataa agaagttgaa tacctcaaat cctttggagc aaactttgga
      841 atgtcttttc aattaattga tgatgctatt gattattcat cttcgaatat agtattgggg
      901 aaaaatattg gtgacgactt taaagaagga aaggttactt tgccgattat acttgcctac
      961 cttcgttcca acaaagcaga aaaagaattc tggaagaaaa caatcgtaca tttaaaacaa
     1021 gaaaaagatg attttcatca tgctataaac ataattaaaa
//
LOCUS       DTVV01000435            1002 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2317892, whole genome shotgun sequence.
ACCESSION   DTVV01000435 DTVV01000000
VERSION     DTVV01000435.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2317892"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="EYO86_05175"
     CDS             complement(<1..153)
                     /locus_tag="EYO86_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIC42455.1"
                     /translation="MNCYEHTFITKQDLSNSQAENLVSKYEGIIKANSGKVLKTEEWG
                     LRNLSHS"
     gene            290..>1002
                     /locus_tag="EYO86_05180"
     CDS             290..>1002
                     /locus_tag="EYO86_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIC42456.1"
                     /translation="MRAVLFPGQGSQYVGMGSDFYKKFDSTKFLFEIVDKTLGFSLSN
                     IIFNGPEAELKLTQNTQPAIMTVGVCIFNVLNQEFGLNLNNAKFFAGHSLGEYTALVC
                     GGSLSIERAAYLLHERGKSMQDAVHPGQGSMLAILGMSVDEVEKEINLLPKGETCEIA
                     NDNSNSQVVVSGKKTAIEILSENLKKKRKKGILLPVSAPFHCSLMKKAAENMKDKIEN
                     TNFLKPKPNIISNVTAKEE"
BASE COUNT          336 a          147 c          180 g          339 t
ORIGIN      
        1 actatgggaa aggtttctta atccccattc ttcagttttt agcacttttc cagagtttgc
       61 tttaattata ccctcgtatt tactgactag attctcagct tgactgttag acaagtcttg
      121 ctttgttata aaagtatgtt cgtaacaatt catttacgtt tttccttggt tagctcgatg
      181 cagcattgag caatccacct tattattggc agaggttgtg gatttatact attaaaattt
      241 tcaatataca actattaaaa tttaattata ttatatttgt tattatctta tgcgtgctgt
      301 tttatttcca ggtcaaggtt cccaatatgt tgggatgggg tcagattttt acaaaaaatt
      361 tgattcgaca aaatttcttt ttgaaatcgt tgataaaact ttaggctttt cactttcaaa
      421 tattattttc aacggtcctg aagcagaatt gaaattgaca caaaatacgc agcctgcaat
      481 tatgactgta ggggtttgta tatttaatgt tttaaaccag gagtttggct taaatttgaa
      541 taatgcaaaa ttttttgcag gacattcctt aggtgaatac acagcgcttg tttgtggagg
      601 atcattgtcc atagaacgag cggcttacct gttgcacgag agaggaaaat ctatgcaaga
      661 tgcagttcat ccaggtcaag gttctatgtt agctatactt ggaatgagtg tagatgaagt
      721 tgagaaagaa attaatttac ttccaaaagg agaaacttgc gaaatcgcta atgataatag
      781 taatagccaa gtagttgtta gtggaaaaaa aacagcaatt gaaattctta gtgaaaattt
      841 aaaaaaaaaa aggaaaaaag gaatactttt gcccgtaagc gcaccttttc attgctctct
      901 aatgaaaaaa gctgctgaaa atatgaaaga taaaattgaa aatacaaatt ttttaaaacc
      961 aaaaccgaac attattagca atgttactgc caaagaagag at
//
LOCUS       DTVV01000436            3739 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2321834, whole genome shotgun sequence.
ACCESSION   DTVV01000436 DTVV01000000
VERSION     DTVV01000436.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2321834"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /gene="ribF"
                     /locus_tag="EYO86_05185"
     CDS             <1..713
                     /gene="ribF"
                     /locus_tag="EYO86_05185"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIC42457.1"
                     /translation="DHKKHLLKDLGIDYYFSLHFDQTIANLSPSEFIEKIIVQQLHVD
                     KLIVGYDFRFGKNREGDIVLLQDHAAIHGFALEIIEPIKDAASAEVYSSTGIRKALQS
                     GDVEKANSMLGRAWTMTGKVVHGDKRAGKMNFPTANILPHSQVYPKKGVYAIHAIMDA
                     DLLKGIANFGERPTVDGKKLLLEVNLFDFDRDIYGKQLTVKFLRFIREEKKFDTISLL
                     KGQITEDIHIAKNYHLRK"
     gene            714..3491
                     /locus_tag="EYO86_05190"
     CDS             714..3491
                     /locus_tag="EYO86_05190"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIC42458.1"
                     /translation="MEYKDTIFLPKTSFEMRANLPMKEPKILEEWNNQNIFKKLREKS
                     KGREKFVLHDGPPYANGHIHMGTALNKILKDVIVRTQQMSGKDSIYVPGWDCHGLPIE
                     WKIEEEYRKKGKNKDDVPVVQFRQECREFAEKWIAVQKKEFRRLGVEGDWDNPYLTMS
                     NQAEAQIVRELGKFLLDESLYKGARPVLWSVVEKTALADAEVEYEDHTSNTIYVRFKV
                     TKSSLTELIDTDIVIWTTTPWTIPGNRALAYGKDLEYALVEVQETEENSLAQVGDLIV
                     LAKELQNTVLSQIGVVKKKVKQTFFGKDLEGTECEHPLKSIGYDFSVRVFEGDFVNLE
                     QGTGIVHVAPGHGADDYNLGIKNNVEIVQTVEDDGKFNHHAKGFVGEHVYKVDLKIAD
                     KLKEFNKLLHLGTLRHSYPHSWRSKAPLIFRNTPQWFISMDKQDLRNKALKSIDETIF
                     YPPQGQARLRSMIETRPDWCVSRQRVWGVPLPLFVSKKDGQPLRDINIINRIANIYEK
                     EGSDTWFTGDPKRFLGEKYSIDDYEQVKDIVEVWFDSGSTHAFCLEQRKDLKWPASMY
                     LEGSDQHRGWFHSSLLVSVGTRGRAPYESVLTHGFVVDGKGRKMSKSLGNFISPDKIL
                     EQYGVDILRLWVVASDYYDDLKLDNSILKAQTDSYRRIRNTFRYLIGNLNGFEENEKL
                     PLDDFPELEKYILHRLWEINQTVEECVNTFNFHLMFTTLLNFCSNDLSAFYFDIRKDT
                     IYCDSFQSTKRRASRTLLDLIFNHLVRWLAPSLAFTCEEAWQARGHTSSIHLEDFIPA
                     KKEYQSTDLQKKWDMIKNVRKVITGALEKKRAEKIIGSSLEAHIKVYVSDEIKKIIAK
                     IHLDEIAITSSYELLSDEGADSGFVMDEIEGVRVEVEKVVGDKCNRCWKFTEALNNNQ
                     ICNRCEKAIQQ"
     gene            3498..>3739
                     /locus_tag="EYO86_05195"
     CDS             3498..>3739
                     /locus_tag="EYO86_05195"
                     /inference="COORDINATES: protein motif:HMM:PF01252.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42459.1"
                     /translation="MNKIIIIVFLLIFDLISKQIIYYSIDLNSFLTITSFLDVAHIHN
                     FGISFGFFSGIVSPWSFILVGIIVTGFIFYMLIKST"
BASE COUNT         1246 a          541 c          771 g         1181 t
ORIGIN      
        1 aagaccataa aaaacatttg ttaaaagatt taggtattga ttattatttt tctttgcatt
       61 tcgatcaaac catcgcgaat ttatcaccat cagaatttat tgaaaaaata attgtgcaac
      121 aactgcatgt tgataaatta attgtagggt atgattttcg ctttggtaaa aacagagagg
      181 gtgacatagt tcttcttcag gatcatgcag cgatacatgg atttgcttta gaaataattg
      241 agcccattaa ggacgcagct agtgcagagg tgtattcttc aacaggtatt agaaaagctc
      301 ttcaaagtgg agatgtggag aaagcaaatt ctatgttggg cagagcttgg acaatgaccg
      361 gtaaagtcgt gcatggagat aaacgggctg gtaaaatgaa ttttcctaca gcaaatatac
      421 ttccccattc tcaggtttat cctaaaaaag gggtttatgc tatccatgcc attatggatg
      481 cagatctgtt aaaaggaatt gctaattttg gtgaaaggcc aactgttgat ggtaaaaagt
      541 tattgttaga ggtgaacctt tttgattttg atagagatat ttatggtaag caattgactg
      601 ttaaattcct tagatttatt agagaggaaa agaaattcga taccatttca ttgttaaaag
      661 gtcaaattac agaagacatt cacatcgcta aaaattatca tctaaggaaa taaatggaat
      721 acaaagatac aatttttctc ccaaaaacat cttttgaaat gagagccaat cttccgatga
      781 aagaacctaa gattttagaa gagtggaata atcaaaatat ttttaaaaag ttgcgagaga
      841 aatcaaaagg tagagaaaag tttgttcttc atgacggacc tccatatgcc aatggtcata
      901 tccacatggg tacagctctc aataagattt tgaaagacgt tatcgtgcgt actcagcaaa
      961 tgtccggaaa agattctatt tacgttcctg gatgggactg tcatggctta ccaattgaat
     1021 ggaagatcga agaggaatat cgcaaaaaag gaaagaacaa agatgacgtg cctgttgtgc
     1081 agtttcgtca agaatgtcga gaatttgcgg aaaaatggat tgccgttcaa aaaaaggaat
     1141 tcagacgatt gggtgtagaa ggtgattggg acaatcctta cttgacaatg tccaatcagg
     1201 cagaagcgca aattgtcagg gaacttggaa aatttttatt ggatgaaagt ctttataaag
     1261 gggcgagacc agtattatgg tcggttgttg aaaaaacagc cttggctgac gcggaagttg
     1321 aatatgagga tcatacatca aatactattt atgttcgctt taaagtaact aaaagttcac
     1381 ttacggaact gattgataca gatattgtta tatggacaac tacaccgtgg acgatacctg
     1441 gcaatagagc tttagcctac ggtaaggatt tagaatatgc tttagttgaa gtacaagaaa
     1501 cagaagaaaa cagtttagct caagtaggcg atcttatagt ccttgcaaaa gaattgcaaa
     1561 atactgtttt gagtcaaatt ggagttgtaa aaaaaaaagt aaaacaaact ttttttggaa
     1621 aagatttaga aggcactgag tgtgaacacc cccttaaatc tataggttat gatttttcag
     1681 tgcgtgtatt tgagggagat tttgttaatc ttgaacaagg aacaggcatt gttcatgtag
     1741 cgcctggaca tggagcggat gattacaatt taggcattaa gaataatgtt gaaattgtac
     1801 aaaccgttga ggacgatggt aaatttaacc atcatgcaaa agggtttgtt ggagagcatg
     1861 tctataaggt tgatcttaaa attgccgata agcttaaaga atttaataaa cttcttcatt
     1921 tgggaacatt acgtcatagc tatccccact catggcgatc aaaagcacca ttgatttttc
     1981 ggaacacacc tcagtggttt atttctatgg acaaacaaga tttgcgaaat aaggcattaa
     2041 aatcgatcga tgagacaatt ttttatcctc cacaaggtca agcacgcttg cgatcaatga
     2101 ttgagacacg tcctgattgg tgtgtatcac gacaacgcgt gtggggggta ccccttccgt
     2161 tatttgttag taagaaagat ggacaaccac ttcgagatat taatattatt aatcgcattg
     2221 ccaatattta cgaaaaagaa ggaagtgata catggttcac aggagatcca aagcgtttct
     2281 taggagagaa atattcaata gatgactatg agcaagttaa agatattgtt gaagtgtggt
     2341 ttgatagcgg ttcgactcat gcattttgtt tagagcaacg aaaagattta aaatggccag
     2401 cctctatgta tttagaagga agtgatcaac accgaggatg gttccattct tctttgctgg
     2461 tatcagtagg aacaagagga cgggctcctt atgaaagtgt tttgacacat ggttttgttg
     2521 tggatggtaa gggaagaaaa atgtctaaat ctcttggtaa ttttatctcc cctgataaaa
     2581 ttttagagca atacggtgtt gatattctcc gattatgggt tgtagcttct gattattacg
     2641 atgatttaaa attagataat tcaatattaa aagcgcaaac agattcatat cgtcgtatac
     2701 gtaatacttt tcggtatctc attggaaatc tcaatggatt tgaggaaaat gaaaagttac
     2761 cgttagatga ttttccagaa ttagaaaaat atattttaca tcgtttatgg gaaattaatc
     2821 aaacagtaga agaatgtgtg aacaccttta attttcattt gatgtttacc actttgttaa
     2881 atttttgttc gaatgattta tcagcatttt atttcgatat tcgaaaagat actatctatt
     2941 gtgattcttt tcaatcaaca aaacgtcgtg cttctcgtac attattggat ttgattttta
     3001 accaccttgt ccgatggcta gccccttcct tggcttttac ttgtgaagaa gcatggcaag
     3061 cacgcggcca tacctcaagc attcatcttg aagattttat tcctgccaaa aaagaatatc
     3121 aaagtactga tttgcaaaaa aaatgggata tgataaaaaa tgttcgtaag gttatcaccg
     3181 gagctttaga gaaaaaacgt gcggaaaaaa tcattggttc aagtttagaa gctcatataa
     3241 aagtttatgt atccgatgag ataaaaaaaa ttattgctaa aatccatttg gatgaaattg
     3301 ccattacttc ttcttatgag ttattgtctg atgaaggcgc tgattcaggg tttgttatgg
     3361 atgagattga gggagtgcgc gtggaagttg aaaaagtggt tggagacaag tgcaatcgtt
     3421 gttggaaatt tactgaagct ctcaataata atcaaatttg caatcgatgc gaaaaagcca
     3481 tacaacaata gaatgttgtg aataaaataa taataatagt ttttcttttg atttttgatt
     3541 taatttccaa acaaataatt tattatagta ttgatctaaa ttcatttttg actatcactt
     3601 cttttcttga tgtcgcgcat atacataact ttgggatttc ttttggtttt ttttctggca
     3661 ttgtttctcc gtggagcttt atattggttg gaataattgt aacagggttt attttttaca
     3721 tgttgataaa gagtacaaa
//
LOCUS       DTVV01000437            1460 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2325285, whole genome shotgun sequence.
ACCESSION   DTVV01000437 DTVV01000000
VERSION     DTVV01000437.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2325285"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="EYO86_05200"
     CDS             complement(<1..200)
                     /locus_tag="EYO86_05200"
                     /inference="COORDINATES: protein motif:HMM:PF01113.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIC42460.1"
                     /translation="MDKIKVGIAGCLGRMGQELVKITTKDKRIKFVGGYDLKNSKTHS
                     NDIFQGANIIIDFSSPAAIKGN"
     gene            complement(247..1374)
                     /gene="dnaJ"
                     /locus_tag="EYO86_05205"
     CDS             complement(247..1374)
                     /gene="dnaJ"
                     /locus_tag="EYO86_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIC42461.1"
                     /translation="MADKDFYEILGVAKNAGEDEIKKSYRKLAMQHHPDRNKNNKLAE
                     KKFKEASAAYEILKDPQKRAAYDQYGHEAFRQGGSGGTHGFGDFSGGFSDIFEEFFGG
                     SSGGSSSRRRGPTSGNDLRYNMAVTLQEAFSGQKSEIRIPGYIGCDMCSSTGSVDKSG
                     PSTCSTCEGHGKVRSTSGFFSIERPCPTCGGEGSSIKNPCLKCSGSGQIKKQKTISVT
                     IPAGVNTGTRIRISGEGEQGQRGAGNGDLYLFVEVQKDALFEREEENIFCQIPISITT
                     AILGGEIEVPTIEGKKARLTIPPGTQSETQFRLRGKGMSVLRQNRRGDMYVETNVEIP
                     VNLNLRQKSILKGFEEEGGTSKAHSPKSQSFFKKIKEVWGI"
BASE COUNT          412 a          321 c          216 g          511 t
ORIGIN      
        1 atattgcctt ttattgctgc aggagatgaa aaatcaatta ttatattagc cccttgaaaa
       61 atatcattag aatgagtttt gctatttttt agatcatagc cacccacaaa tttaatgcgc
      121 ttgtcttttg tggttatctt aaccaattct tggcccattc tgccaagaca tccggcaata
      181 ccaaccttga ttttatccat aatatttata tatacttttg agaaggcaat aagtgaagaa
      241 tttatgttaa atcccccaaa cttctttaat ttttttaaaa aaactttgtg attttggact
      301 gtgggcctta gatgtacccc cttcttcttc aaaacctttc aaaattgatt tttgtcgtaa
      361 attaagattt acgggaattt ctacattggt ttctacatac atatccccgc gtctgttttg
      421 acgaagaacg ctcattccct ttcctcgaag tctaaactgt gtttcagatt gtgttcctgg
      481 tggaatagta agtctggctt ttttcccttc aattgtaggc acttcaattt ctcctcctaa
      541 aattgctgtg gtaattgaaa tgggaatttg acaaaatata ttttcttctt ctctttcaaa
      601 caaagcgtct ttttgtacct caacaaatag atacaagtct ccattaccag cccctctctg
      661 tccctgctct ccttcacctg aaattctaat acgggttccc gtatttactc ctgcaggaat
      721 agttactgaa atagtttttt gtttttttat ttgacctgag ccactacatt ttaagcaagg
      781 atttttaatt gatgaacctt cgccaccaca ggttgggcaa ggacgctcaa tagaaaaaaa
      841 tcctgatgtc gatctaactt taccatgacc ctcgcaagtt gaacatgtac ttggaccaga
      901 tttatccacg cttcctgtag atgaacacat gtcacaacca atataacctg gaatacgaat
      961 ttcagatttt tgaccagaaa acgcttcttg aagagtaaca gccatattat atctcagatc
     1021 gtttccgcta gtaggtcccc ttcttcttga agaagatcct ccagaactac caccaaaaaa
     1081 ctcttcaaat atatcagaaa aaccgccgct aaaatcaccg aaaccatggg tgcctccaga
     1141 gccaccttga cgaaaagctt catgtccata ttgatcgtat gcagctcgtt tttgaggatc
     1201 ttttaatatt tcataagcgg cgctagcttc tttaaatttt ttttcagcta acttattatt
     1261 tttattgcga tcaggatgat gttgcatcgc aagctttctg taagattttt ttatctcatc
     1321 ttcgccagca ttttttgcaa cgccaagaat ttcatagaaa tctttatcgg ccatccaacc
     1381 cctttgctct ttaaagaatt aacttaaatt aatctttttt gttttcatcc acctcttcaa
     1441 actccgcatc aacaactttg
//
LOCUS       DTVV01000438            1146 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2330921, whole genome shotgun sequence.
ACCESSION   DTVV01000438 DTVV01000000
VERSION     DTVV01000438.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2330921"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="EYO86_05210"
     CDS             complement(<1..1136)
                     /locus_tag="EYO86_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC42462.1"
                     /translation="MQKKYQSPWFVEALQSEGKLEINKLETDMTADMCIAGGGFTGLW
                     TALKIKEKKPSINIAIIEKDLCGSGASGRNGGCMIPQSTKFIAMKKVVGVKDAKKMVK
                     ASEDAVYNIRDFCMQNNIDAKIRINGVAYTATNKSHEGAFENLIENLKEEKINSWERL
                     SKKKIQELTGSERNIDGYYSAIGGSLQPALLIRGLKKIAEKRGIKIFEHSAMLSYKEN
                     NNIIVKTKNGSIECKKLIVAINAWTPSFFPFLSRSVILVSSDMIISEPIKDKLENLKL
                     NNGLVILDSNLFTHYCRTTTDGRIILGKGGNTFAFNNKVIPSYDGPSKIETFLKKSLV
                     SFFPSLKNVKITKSWNGPSERTKTGFPFFGYHPKNSNILYGFGYS"
BASE COUNT          350 a          200 c          146 g          450 t
ORIGIN      
        1 gaatatccaa atccatataa aatatttgaa ttttttggat gatatccaaa aaaaggaaat
       61 cctgtttttg ttctttccga tggaccattc cacgatttag taatttttac attttttaaa
      121 gaaggaaaaa aactaacaag tgattttttt aaaaaagttt caatcttact aggcccatca
      181 taggaaggaa tgactttatt attgaatgcg aaagtattac ctcccttacc cagtattatt
      241 ctgccatcag ttgtagtacg acaataatga gtgaataagt tagaatctag aatcactaat
      301 ccattattta attttagatt ttccagttta tcttttattg gttcacttat aatcatatct
      361 gatgaaacaa ggattacact tcttgataaa aaaggaaaaa aagatggtgt ccatgcgttg
      421 attgcaacaa ttagcttttt acattcaata gatccatttt tagttttaac aataatatta
      481 ttgttttctt tgtaggataa catagcgctg tgttcaaaaa tttttatacc tcttttttct
      541 gctatttttt ttaatccccg tattaataaa gctggttgca aacttcctcc gatagcagaa
      601 tagtatccat caatatttct ttctgatccg gttagttctt gtatcttttt ttttgataat
      661 ctttcccaac tatttatttt ttcttcttta aggttttcaa taagattttc aaaagcacct
      721 tcatgagatt tattagtggc agtatatgct actccattga ttctgatctt ggcatcaata
      781 ttattttgca tgcaaaaatc tcttatattg taaactgcat cttcacttgc cttaaccatt
      841 tttttagcat cctttacacc aactactttt ttcatagcaa taaattttgt agattgagga
      901 atcatacatc ccccatttct ccctgatgca ccgctgccgc acaaatcttt ctcaattatt
      961 gcgatattaa ttgaaggttt tttttcttta atttttaatg cagtccaaag gccagtaaat
     1021 ccacctcctg caatacacat atctgcagtc atatcagttt ctaatttatt aatttctaac
     1081 ttgccctcag attgtaatgc ttcaacaaac caaggagatt gatatttttt ttgcatgaag
     1141 atttta
//
LOCUS       DTVV01000439            1426 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2331636, whole genome shotgun sequence.
ACCESSION   DTVV01000439 DTVV01000000
VERSION     DTVV01000439.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2331636"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="EYO86_05215"
     CDS             <1..410
                     /locus_tag="EYO86_05215"
                     /inference="COORDINATES: protein motif:HMM:PF00263.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilQ"
                     /protein_id="HIC42463.1"
                     /translation="TAKITQGTQIPYQVAAEGANISNTEFVDAALSLEVTPSIVGDGN
                     IVLDLIVNNDSIGTVTVGTQPTIDKMEIRTKLLVEDGSIVVIGGIFSDKDSKTKALTP
                     GLGRLPIIGGLFRHKLKDVEKTQLLVFIAPRMV"
     gene            455..>1426
                     /locus_tag="EYO86_05220"
     CDS             455..>1426
                     /locus_tag="EYO86_05220"
                     /inference="COORDINATES: protein motif:HMM:PF05157.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42464.1"
                     /translation="MLNISESASINIATMLLSHKKITEKQMKQLQILSKESGQGIVSI
                     ILDKNFATETDIAETIAKSYDLPKIKITLQNVEPQAISELPKDFILTNQVLPFSMKDG
                     SLQIAIADPTRLSISSEIKTITDKNIDIFVTKLSDLEKGLKALNISSSSQSTIPSSQT
                     SRVKLGTKDTSKDIETTSDVIRFVDDVLIKAIKNGSSDIHIEHYRAGPRIRLRLDGIM
                     SVEKELTRFLIKNYPAVVTRLKIMAETNISERRLPQDGAIQFNHKGNECDFRVSFLPT
                     KYGERVVLRLLKKEAIQLELEKLGFEKDHLEKIKSAITAPQGLILVTG"
BASE COUNT          541 a          230 c          246 g          409 t
ORIGIN      
        1 aaacagccaa aattactcaa ggaacacaaa ttccctacca agtagcagct gaaggagcca
       61 atatttccaa cactgaattt gttgatgctg cactcagttt agaagtaact ccaagtattg
      121 tgggagatgg caacattgtt ttagatctca tagtaaataa tgacagtatt ggaacagtaa
      181 ccgttggtac ccagcctaca attgataaaa tggaaattag aacaaagctt cttgtagaag
      241 acggttctat tgttgtaatt ggtggtatat tttcagataa agattccaaa acaaaagcac
      301 tgactcctgg attaggaaga ttacctataa taggaggatt atttcgccac aaactaaaag
      361 atgtagaaaa aacacaatta ctagttttta ttgcacctcg catggtttaa tattatcttt
      421 atcttaagag taaaaacgta ggaaaaaaaa aactatgcta aatattagtg aaagcgcctc
      481 tataaacatt gcaacaatgt tgctgtcgca caaaaaaata acggaaaaac aaatgaaaca
      541 acttcagatt ttgagcaaag aaagcgggca aggaatcgta agtataatat tagataagaa
      601 ttttgctacc gaaactgata tagcagaaac aattgctaaa agttatgatt tgcccaaaat
      661 aaaaataacc cttcaaaatg tagaacctca agccatttca gaattgccaa aagattttat
      721 ccttactaat caagtgcttc ctttttcaat gaaagatggc agtttgcaga ttgccattgc
      781 agaccctacg cgcttatcta taagtagtga aataaaaaca attactgata aaaatattga
      841 tatttttgta actaaattat ccgatttaga aaaaggatta aaagcattaa atatttcctc
      901 ttcctcacaa agtacaatac caagctcaca gacatcacgt gtaaaacttg gaacaaaaga
      961 tacatctaag gacattgaaa ccacatcaga tgtaattaga tttgtggatg atgttttgat
     1021 taaggctatt aaaaatggct ctagtgatat tcatatagaa cattatcgtg ctggtcctag
     1081 aatccgattg cgcctggatg gtataatgtc agtggaaaaa gaattaacca ggtttttaat
     1141 taagaattat ccagctgttg taacccgttt aaaaataatg gccgaaacca atatttcaga
     1201 aagacgtctg cctcaggacg gagctattca atttaatcac aagggtaatg aatgtgattt
     1261 tcgtgtttcc tttttgccaa ccaaatatgg ggaacgtgta gtacttcgtt tattaaaaaa
     1321 agaagctatt caattagaat tagaaaaact aggatttgaa aaagatcatt tagaaaaaat
     1381 caaatctgca ataacagccc cgcaaggttt gattttagta actggt
//
LOCUS       DTVV01000440            2218 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2332118, whole genome shotgun sequence.
ACCESSION   DTVV01000440 DTVV01000000
VERSION     DTVV01000440.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2332118"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="EYO86_05225"
     CDS             <1..202
                     /locus_tag="EYO86_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIC42465.1"
                     /translation="PSLEEIDNIHSFIKNEIQNIENYKILYGGSVNSNNASEIMDLKN
                     VDGVLVGGASLDALEYKKIMNS"
     gene            246..545
                     /gene="secG"
                     /locus_tag="EYO86_05230"
     CDS             246..545
                     /gene="secG"
                     /locus_tag="EYO86_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIC42466.1"
                     /translation="MTTLIVIQIILAVLLVALVLLQGSDNEGLGLGGGGGMGGMMSAR
                     GSANLLSRLTAITATLFMVMSVVLTITASVGSDKNILESLPEISTSEEPSIPDNN"
     gene            597..>2218
                     /locus_tag="EYO86_05235"
     CDS             597..>2218
                     /locus_tag="EYO86_05235"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIC42467.1"
                     /translation="MHYVFITGGVVSSLGKGLASASLASLLQLRKFKVRIRKLDPYLN
                     VDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTNLSASQSDSISSGKIYSNVLRKER
                     RGEYLGSTIQVIPHVTDEIKSFIHSDLKNEDIVIYEIGGTVGDIESLPFLEAIRQIRN
                     DRKISSSLIHMTYVPYISSAGELKTKPTQHSVKELLNAGIQPDIIMCRCEKSIDQEAL
                     KKISLFCNVKIENVIPAMNANSIYEVPSLYCKAGLDTALLNELGYDPKKYPLKLSAWD
                     EVVKKFKNTTNDVTIGVVGKYTDLKDSYKSLNEALIHGGLSNETSVSINWINSERLKN
                     NEIRNELTKVHGILVPGGFGERGINGKLCSITFARENDIPYFGICLGMQLAVIEIAKN
                     VLKINDANSTEFGKTNNPVIGLMTEWVRDNTKFTRSEESDKGGTMRLGSYPCNLKKGS
                     LVYSIYDTIQISERHRHRYEVNTKYMDLFNKNNFCYSGMSPDNLLPEILESTEHPWFI
                     GVQFHPELKSRPQSPHPLFVSFIEAAVKNKKN"
BASE COUNT          754 a          316 c          410 g          738 t
ORIGIN      
        1 cccttcttta gaagaaattg acaatattca ttcttttatt aaaaatgaaa ttcaaaatat
       61 tgaaaactat aaaatacttt atggaggatc tgttaattca aataatgctt cagaaattat
      121 ggatttaaaa aatgtagatg gggttttggt tggaggagcg tctttagacg ctctagaata
      181 taagaaaata atgaattctt gatgattaat cttgaataac ttaattagat aaatataagg
      241 ttttcatgac aacattaatt gttattcaaa taatattggc agtattgctt gttgctctcg
      301 ttttactcca aggttctgat aatgaaggat tgggactagg tggaggaggt ggtatgggtg
      361 gaatgatgtc agctcgtgga tcagcaaact tattatctcg tttgacagca attacagcta
      421 ccttgtttat ggttatgagt gtagtattaa caataactgc ctctgttggt tccgataaaa
      481 atattcttga atccttacct gaaatttcta cttcagaaga accgtcaatt ccagataata
      541 attaagtaaa attaacttga tataattgtt tcatttatgt atcacggtat tccgtaatgc
      601 attatgtctt tattacgggt ggggttgtct catctttggg taagggttta gcctcagctt
      661 ctcttgcctc tcttctacag cttagaaaat ttaaagtcag aataagaaaa ttggaccctt
      721 atttaaatgt tgacccgggt acaatgagtc catatcaaca tggtgaggtt tttgtcactg
      781 atgacggcgc tgaaacagat ttggatctgg gacattatga aagatttacc aatctttctg
      841 caagtcaatc tgatagtatt tcttctggca agatttattc taatgttctt cgcaaggagc
      901 gaagaggtga atacttggga tctactattc aggtcatacc tcatgttacg gatgaaataa
      961 aaagtttcat tcatagtgac cttaaaaatg aagatattgt tatttatgaa attggaggta
     1021 cagttggtga tatagaatca cttccttttt tggaagctat cagacaaata cgtaacgata
     1081 gaaaaatctc atcttcttta attcatatga cttatgtacc ctacatatct tctgcaggtg
     1141 aattgaagac aaaaccaact caacattcgg tgaaggaatt attaaacgca ggtattcaac
     1201 ctgatattat tatgtgtcga tgtgaaaaat caattgatca agaagcgcta aaaaaaatat
     1261 ctcttttttg caatgtaaaa atagaaaatg taattccagc gatgaatgca aattctattt
     1321 atgaagtgcc atcgttatat tgtaaagcag gtttagatac agctttgtta aatgaattag
     1381 gttatgatcc taaaaaatat cctttaaaat taagtgcttg ggatgaagtg gttaaaaaat
     1441 ttaaaaatac tactaatgat gttacgattg gagtagtagg aaaatacact gacctaaaag
     1501 atagttataa gtctttaaac gaagccttaa ttcacggagg gctatctaat gaaacctctg
     1561 ttagcattaa ttggattaac tcagaacgtc ttaaaaataa tgagataaga aatgaactta
     1621 ctaaggtcca tggtatttta gttcccggcg gttttggaga acgaggtatt aatggcaaat
     1681 tatgtagcat tacatttgct cgagaaaatg atattcccta ttttggtatc tgtcttggaa
     1741 tgcagttggc tgttattgaa attgctaaaa acgttttaaa aataaatgac gctaattcta
     1801 ctgaatttgg aaaaaccaat aatccggtta taggtttgat gacagaatgg gtcagagata
     1861 atactaaatt cacacgttcc gaagaaagtg ataaaggggg aacaatgaga ttgggctctt
     1921 atccttgtaa tttaaaaaaa ggttctttag tttattcaat atacgatact attcagattt
     1981 ctgaaagaca tcgacaccga tatgaagtaa acacaaaata tatggattta tttaataaaa
     2041 ataatttttg ctactcaggc atgtcccctg ataatttatt gccagaaata ttagagtcta
     2101 cagaacatcc ttggttcatt ggtgtgcaat ttcatcctga gttaaaatct cggccacaaa
     2161 gtccacatcc tttgtttgtt tcgtttattg aagcagcagt aaaaaacaaa aaaaatga
//
LOCUS       DTVV01000441            3325 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2337151, whole genome shotgun sequence.
ACCESSION   DTVV01000441 DTVV01000000
VERSION     DTVV01000441.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2337151"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="EYO86_05240"
     CDS             <1..397
                     /locus_tag="EYO86_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017971500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42468.1"
                     /translation="PNIPDEQKPAIGKVIAPAALFWFRWAALSTIITGLIVAYLSGYV
                     NQAMTLGLVGETDPKSITIGIGMWLGIIMAYNVWMIIWPNQKKALGIIDATPEEKVKS
                     ARTAMLLSRTNTLLSFPMLLTMVGAQNLY"
     gene            complement(389..1297)
                     /gene="secF"
                     /locus_tag="EYO86_05245"
     CDS             complement(389..1297)
                     /gene="secF"
                     /locus_tag="EYO86_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIC42469.1"
                     /translation="MFGINKIIPADTNYPFMDYRKIFIISSLILILSSVLLLSFKGLN
                     LGIDFKGGTLIEISTKNSDIAELRRILSPNFNDVSLQEFGNSETIIIRLQNETNVESI
                     ETVNQVKNLINDKVSEFRRSEFVGPTVSSELLWKGLQAITFALLAILTYIWLRFDWQF
                     GFGAVVALSHDVLFTLGVLSLFNIDFSLSSIAAILTICGYSLNDSVVIYDRIRENLRK
                     YKKLELSELFNVSINNTLSRTIITSLTTLIALFSLYIFGGEVIKPFAFAMIIGVIIGT
                     YSSIYIAVPTLLIFKFRPQEEDNKLI"
     gene            complement(1297..2847)
                     /gene="secD"
                     /locus_tag="EYO86_05250"
     CDS             complement(1297..2847)
                     /gene="secD"
                     /locus_tag="EYO86_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIC42470.1"
                     /translation="MKNYPLWKATLVILVISLAIIFSLPSLLYKENSNNWFFDNKINL
                     GLDLQGGSYLLLEVQTEVLMQEELNNISDSIQLIARGLQTNIINIISEEDEIQLRFSN
                     NDKLEEIREKFKNNYRNVNVTINNNTMKIVINEIFKKNIKESAIKQSLEIVRKRIDES
                     GTKEPLIQRSGKKRILLQLPGIKDPERIKELLGTTAKLTFHMVDDEDTISLKANRAPF
                     GKIIVADFYNPEIKYLVEKKSRVGGENLVDAKASFDPQEGHAVSFRFDTTGAQKFGKA
                     TSENVGKRFAVILDGVVITAPVINTAITGGSGIITGNFTSQEAADLSVLLRAGALPAP
                     LSIIEERSVGPGLGADSIASGKIAAIIGMICVCIFMILIYGVFGLLANLSLLANLFII
                     VSLLGTIGATLTLPGIAGIVLTIGIAVDANVLVFERIKEEANKTTSALEAVKNGFDKA
                     LSTILDANITTLIAALLLFVFGSGPIKGFSITLSLGVLASMFTAIMFTNFLIYLWFNF
                     SNKKEINL"
     gene            complement(2853..3200)
                     /gene="yajC"
                     /locus_tag="EYO86_05255"
     CDS             complement(2853..3200)
                     /gene="yajC"
                     /locus_tag="EYO86_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIC42471.1"
                     /translation="MDQMGAFLPLILIFGVFYILLIRPQQKKVKRHREMLNNLRRGDK
                     IITSGGIIGNINKVSENRELQVEISDGVEIKIAPGMVADLYVVPEKTKTGTSTPKSEN
                     KGGFLSGIFGNKK"
BASE COUNT         1177 a          564 c          464 g         1120 t
ORIGIN      
        1 gccaaatata ccagatgaac aaaaaccagc aattggcaaa gtaatagccc ctgctgcact
       61 tttctggttt aggtgggcag ctctttccac aattataaca ggtctaattg ttgcttatct
      121 aagcggatat gtaaatcaag caatgacact aggattagtt ggagaaacag atccaaaaag
      181 cataacaatt ggcattggta tgtggttagg aattataatg gcttacaatg tttggatgat
      241 catatggcct aatcagaaaa aagcattagg aataattgat gcaactccag aagaaaaagt
      301 aaagagtgca agaacagcga tgctgttatc aagaacaaat actttgcttt catttcctat
      361 gctcttaaca atggtaggtg cacaaaattt atattaattt attatcttct tcttgcggtc
      421 taaacttgaa tataagtaaa gttgggaccg caatatatat agaagagtaa gtacctataa
      481 taactccaat aatcatagca aaagcaaaag gcttgattac ttcacctcca aaaatataaa
      541 ggctaaataa agcgattaat gttgttaaac tagtaataat tgttcttgat aatgtgttat
      601 taatagagac attgaataat tctgataatt ccaatttttt atattttctt aggttttctc
      661 ttatgcgatc atagataaca acagaatcgt ttaatgagta tccacaaatg gtaagaatag
      721 cagcaatact cgataaggaa aaatcaatat taaataaaga taagacgcct aatgtaaaaa
      781 gtacatcatg agaaagagca acaactgctc caaatccaaa ttgccaatca aatctaagcc
      841 aaatatatgt taaaatagct aataaggcga aggtaatagc ttgtaaccct ttccataata
      901 gttctgaact gaccgtaggg ccaacaaatt cagaacgtct aaactcacta actttatcat
      961 taattaaatt cttaacctga ttaacagttt caatactttc aacattagtt tcattttgca
     1021 atctgataat aatcgtttca gaattaccaa attcttgaag tgaaacatca ttaaaatttg
     1081 gtgaaagaat tcttcgtaat tctgcaatat cagaattttt tgtactaatt tctatgaggg
     1141 tgcccccttt aaaatcaata cctaaattta accctttaaa cgatagtaat aaaactgaac
     1201 ttaaaattaa aataagtgag ctaataataa atatttttct ataatccata aaaggatagt
     1261 ttgtatcagc tggaataatt ttattgatac caaacattat aaattaatct cctttttatt
     1321 tgaaaaattg aaccataaat aaataagaaa gttcgtaaac ataatagcag taaacataga
     1381 agccagaaca cctaaagata aagttattga gaaaccctta attggcccag atccaaaaac
     1441 aaataaaagg agtgctgcaa ttaaagtagt aatgttagcg tctaaaatag ttgaaagcgc
     1501 tttatcaaat ccatttttta cagcttctag agcgcttgtt gttttatttg cttcttcttt
     1561 aattctttca aaaactaaaa cattagcatc tacagctata ccaatagtta gcacaatgcc
     1621 ggctatacca ggcaatgtca gtgttgcacc aatggtaccc aataatgata caataataaa
     1681 taaattggcc aataaagata aattagcgag caaaccaaac accccataaa ttaaaatcat
     1741 aaatatacat acgcatatca tgcctataat tgctgcaatt tttcctgaag ctatggaatc
     1801 agctcccaaa cctggtccaa cagatctttc ttcaataata cttaatggtg caggtaatgc
     1861 tcctgctctt aataagactg ataaatcagc tgcttcttgg gatgtaaaat taccggtaat
     1921 aattcctgag cctccggtaa tagctgtatt tataacaggg gcagtgataa caacaccatc
     1981 aaggataaca gcaaaccttt ttccaacatt ttcactggtt gcttttccaa acttttgagc
     2041 tcctgtcgta tcaaatcgga aagaaacggc atgtccttcc tgaggatcaa aagatgcttt
     2101 tgcatcaact aaattttccc ctcccacacg tgattttttt tctactaaat attttatttc
     2161 tgggttgtaa aaatctgcaa caattatttt tccaaaagga gctctatttg cctttaggga
     2221 tatcgtatct tcatcatcaa ccatatgaaa agttaactta gctgtagtgc ctaatagttc
     2281 tttaatcctt tcaggatctt tgatacctgg tagctgtagt aaaattcttt ttttgccaga
     2341 gcgctgaatt aaaggttctt ttgttccaga ctcatcaata cgtttacgta ctatttcaag
     2401 agactgcttt attgcagatt ctttaatatt ttttttgaaa atttcattaa ttacaatttt
     2461 cattgtatta ttattaatcg taacattgac attgcgataa ttattcttaa atttttctct
     2521 tatttcttct aatttatcat tattagaaaa ccttaattga atttcatctt cttcagatat
     2581 gatatttatt atgtttgttt gtaatcctct agctattaac tgaattgaat cactaatatt
     2641 attcagctct tcttgcatta gaacttcagt ttgaacttcc aacaataaat aagaacctcc
     2701 ctgcaagtct aagcctaaat ttattttatt atcaaaaaac caattattag aattttcctt
     2761 ataaagtaat gatggtaatg aaaaaattat tgccaaagaa ataactagaa tgaccaacgt
     2821 ggctttccac aaaggataat ttttcatgta tattattttt tattgccaaa aattcctgaa
     2881 agaaaaccgc ctttgttttc tgattttggt gttgaagtgc ctgtttttgt tttttcagga
     2941 acaacataca aatctgcaac catacctgga gcaattttta tttcaacacc atcggagatt
     3001 tctacttgta attctctatt ctcactcacc ttattgatat taccaataat acctccagag
     3061 gtaattattt tgtctccccg acgtaaatta ttaagcattt ctctatgtcg ctttaccttc
     3121 ttttgttgtg gacgaattaa caaaatataa aaaaccccaa aaattaaaat tagaggtaaa
     3181 aaagctccca tttgatccat aaatttccta ttattattta atttgaggat ttacttatac
     3241 tgtgtaaaag aaaaatgcaa ttatagttca gaagagataa tcatgggttt cattacattt
     3301 tttttactta aaaatctatt aaata
//
LOCUS       DTVV01000442            1005 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2337607, whole genome shotgun sequence.
ACCESSION   DTVV01000442 DTVV01000000
VERSION     DTVV01000442.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2337607"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="EYO86_05260"
     CDS             <1..93
                     /locus_tag="EYO86_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_815366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42472.1"
                     /translation="KPGDKLERYEPLVEVVTDKVTTDIPSPFDG"
     gene            189..689
                     /locus_tag="EYO86_05265"
     CDS             189..689
                     /locus_tag="EYO86_05265"
                     /inference="COORDINATES: protein motif:HMM:PF00694.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42473.1"
                     /translation="MIRGVFSNMKIPSVYELSKRHKKMKVPSIIIAGKEYGTGSSRDW
                     AAKGVSLLGVKAVVFKTIERIHRTNLIGMGVLPLQFLGKDNAKSLKITGTETFDFIDL
                     ESDLIPNKIIRCFITYKNKQKKIIKLKIRIDSKKELEYLTNGGILPYVLKQICNKENS
                     QNYDNL"
     gene            710..>1005
                     /locus_tag="EYO86_05270"
     CDS             710..>1005
                     /locus_tag="EYO86_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HIC42474.1"
                     /translation="MPYVVNQKCIKCKFTDCVEVCPVDCFYEGENMLIIHPDECIDCG
                     VCEPECPVDAITPDTDDEAKDWTEINKKFSETWPNITKKKQTFSKEEQDKWAKE"
BASE COUNT          384 a          124 c          194 g          303 t
ORIGIN      
        1 aagccaggag acaaattgga gcgctatgaa cctctggtgg aggtggtaac agataaggtg
       61 accactgata tcccatcgcc ttttgatggc taacgcggga tgcaatatga tatgctggag
      121 ataaaaaaaa taaagttaaa acttatgatt ttaatacgta tggtgcaaga agaggcaatc
      181 atgaagttat gataagaggt gttttttcaa atatgaaaat accttctgtt tacgaattgt
      241 caaaaaggca taaaaaaatg aaagtcccct caataattat tgcaggaaag gaatatggaa
      301 caggttcttc acgagattgg gctgccaaag gagttagttt attaggtgtt aaagctgtag
      361 tatttaaaac aattgagaga attcacagaa ccaatcttat tggtatgggt gttttacctt
      421 tacaatttct aggtaaagat aatgcgaaaa gtcttaaaat tactggtaca gaaacttttg
      481 actttattga tttggagagt gatttaatac ccaataagat aataagatgt tttattactt
      541 ataaaaataa acaaaaaaaa attattaaac ttaagataag aattgatagt aaaaaagagc
      601 ttgaatattt aacaaatggg ggcattttac cctatgtttt gaaacagatt tgtaacaaag
      661 aaaatagcca aaattatgat aatttgtgat ataatactat attttcctta tgccttacgt
      721 tgttaatcaa aaatgtatta aatgtaaatt tacagactgc gtcgaagttt gtcctgtaga
      781 ttgcttttat gaaggtgaga atatgttaat tattcatcct gatgaatgta ttgactgcgg
      841 tgtttgcgag cctgaatgtc cggttgatgc aataactcca gatacagatg atgaagcaaa
      901 agattggact gaaataaata aaaaattttc tgaaacatgg ccaaatatta ccaaaaaaaa
      961 acaaactttt tccaaagagg aacaagataa atgggctaaa gagaa
//
LOCUS       DTVV01000443            1116 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2340845, whole genome shotgun sequence.
ACCESSION   DTVV01000443 DTVV01000000
VERSION     DTVV01000443.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2340845"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1116
                     /locus_tag="EYO86_05275"
     CDS             <1..>1116
                     /locus_tag="EYO86_05275"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00204.23,HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIC42475.1"
                     /translation="PGMYIGNTNEEGLHHLVNEVLDNSIDEIVAGHAKNISFYYAKDK
                     SITIKDDGRGIPVDFHPKYKNKRALEIVLTTLHAGGKFSESAYKTSGGLHGVGISVVN
                     ALSERMEVKVYNKAKVYSQNYSRGLAKSKITIGKCKKYLKGTEIRFIPDNEIFEIVMF
                     SPKKLHSFIKMKAVLVKGAKISFAVDKDLIHDTTPNHAEFYYPKGIVDLMKENFENRK
                     KVISKFFYANLDLNSKEKCEIYIAFNEGQQSSMESFCNTIKTPDGGSHENALKNSVMK
                     SIKLYGKKNQLSKSVNVTPNDLFDFSDSFIYIFINSPSFEGQTKKRIMMPKIQKQLEE
                     KIQNQFLLWLNSNKKESIKLLETLIERSLLRTDLSKFK"
BASE COUNT          435 a          152 c          185 g          344 t
ORIGIN      
        1 cctggaatgt atattggcaa taccaatgaa gagggattgc accatctggt aaatgaagta
       61 ctagataatt ccatcgatga aatagtagca ggtcatgcca aaaatatttc tttttactat
      121 gcaaaagata agtcaataac aataaaagat gatggacgcg ggattcctgt tgattttcat
      181 cccaaatata aaaataaaag ggctttagaa atagtattaa cgaccttaca tgcaggggga
      241 aaattctcag aaagtgctta taaaacatca ggtggattac atggtgtggg aatttcagtt
      301 gttaatgcct taagtgaaag aatggaggta aaagtttata ataaagccaa agtttactca
      361 cagaattact cacgtggact ggctaaaagt aaaatcacta ttggaaagtg caaaaaatac
      421 ctcaagggta ctgagatacg ttttattcct gataatgaaa tttttgagat tgttatgttc
      481 tctcctaaaa aacttcattc atttataaaa atgaaagctg tcttagttaa gggagctaaa
      541 atttcatttg cagtagataa agatttaatt catgacacaa ctccaaatca tgctgaattt
      601 tattatccca aaggaattgt tgatttgatg aaagaaaact ttgaaaacag gaaaaaagtt
      661 atttctaaat ttttttatgc taatttagat ttaaattcaa aagaaaaatg tgagatttat
      721 attgctttca atgaaggtca gcaatcttca atggaatcat tttgcaatac cattaaaaca
      781 cccgatggcg ggtcacacga aaatgcctta aaaaactcag ttatgaaatc aataaaactt
      841 tacggcaaaa aaaatcaact atcaaaatcg gtaaatgtaa cgccaaatga tttatttgat
      901 ttctctgatt ctttcattta tatctttatc aactctccta gttttgaggg acaaactaag
      961 aaaagaataa tgatgcctaa aattcagaag caattagaag aaaaaattca aaatcagttt
     1021 ttgttatggt taaatagtaa taaaaaagaa tctataaaat tgttagaaac tttaattgaa
     1081 cgttcgttac tgaggacaga cttgtcaaaa tttaaa
//
LOCUS       DTVV01000444            1447 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2349682, whole genome shotgun sequence.
ACCESSION   DTVV01000444 DTVV01000000
VERSION     DTVV01000444.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2349682"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="EYO86_05280"
     CDS             <1..360
                     /locus_tag="EYO86_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC42476.1"
                     /translation="KTGFYMTPMKDGIRVAGTVEIAGLKKPMNKNMLSMIEKTARRIL
                     PQLGKVKSEWMGFRPTLPDSLPVVGESKKCKNVYYAFGHQHLGLSLAAITGKVIQSLI
                     ENRTSNINIDALNPYRF"
     gene            435..719
                     /locus_tag="EYO86_05285"
     CDS             435..719
                     /locus_tag="EYO86_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIC42477.1"
                     /translation="MNSLLGLIIQIINLYQFVLLIYIIATWLISFNIINTSNHFVYSV
                     MKALYRLCEPSLRYIRQFLPRFGSIDISPIIVYLLLWFLKSLLIEYWPRY"
     gene            719..>1447
                     /locus_tag="EYO86_05290"
     CDS             719..>1447
                     /locus_tag="EYO86_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIC42478.1"
                     /translation="MTHIIDGKEISQNLRDKLKKDISGLKNKYNAVPGLAVVQVGNVA
                     ASSVYVKAKTKSAHEVGIEIIDHHLDESTSQDQLLELINTLNNQDNVNSILVQLPLPK
                     HMNEQTILDSIHPDKDADGFHPFNVGKLSIASHNDENLLIPCTPYGCLLMLKGLKIDL
                     VGKNAVVIGRSNIVGKPMAQLLIKESCTVTVAHSKTKNLPDVCRNADILVAAVGRPEM
                     IKGDWVKKGAIVIDVGINRIATQTD"
BASE COUNT          508 a          214 c          256 g          469 t
ORIGIN      
        1 aaaacaggtt tttatatgac accaatgaaa gatggaatta gagtcgctgg aactgttgaa
       61 attgcaggct taaaaaaacc tatgaataaa aatatgcttt ctatgataga aaaaactgcg
      121 aggaggatac taccgcaact aggaaaggtt aaaagtgaat ggatgggatt tagacctacc
      181 ctccctgatt cattgccagt agttggtgaa tcaaaaaaat gtaaaaatgt ttattatgcc
      241 tttggccatc aacatcttgg tttatctttg gcagctatta caggtaaagt aatacaatca
      301 ctaattgaaa acagaactag taatattaat atagacgcct tgaatcctta tcgattttaa
      361 aatttacttt ttttattatt gttatatttg caactaataa ttgataatat ttaaaaatta
      421 attagtacaa agccatgaat tcattactag ggctcatcat tcaaataatt aatttatatc
      481 agtttgtgtt gctgatttat atcattgcaa catggttaat tagttttaat attatcaata
      541 catccaatca ttttgtctat agtgtgatga aagcccttta tcgtttatgc gaacctagtt
      601 tgcgatatat aagacagttt ttgccaagat ttggttcaat tgatatttct cctataattg
      661 tttacttatt attgtggttt ttaaaaagtt tattaattga gtattggccg agatattaat
      721 gacacatatt attgatggaa aggaaatttc tcagaatctt cgtgataaac ttaaaaaaga
      781 tatcagtggt cttaaaaaca aatacaatgc ggttccagga ttagcagtag tacaagttgg
      841 caatgttgca gcaagcagtg tgtatgtgaa agctaaaaca aaaagtgccc atgaagtagg
      901 aattgagatc attgatcatc acctagatga atcaacttcg caggatcaat tattagagtt
      961 aattaataca cttaacaatc aggataatgt taacagtata ttagttcaat tacccttgcc
     1021 caaacatatg aacgaacaaa ccatacttga ctctatacac cccgataaag atgcagatgg
     1081 ttttcatcct tttaatgttg gaaaattatc tattgcaagc cataatgatg aaaacctact
     1141 tataccctgc acaccgtatg gatgtctctt aatgttaaaa ggtcttaaaa ttgatcttgt
     1201 ggggaaaaat gccgttgtca taggaagatc aaacattgtt ggtaaaccta tggcacaatt
     1261 attaataaaa gaatcttgta ctgtcactgt tgctcattct aaaacaaaaa atctgccgga
     1321 cgtttgtaga aatgccgata tattagtggc cgctgttgga aggcctgaaa tgattaaggg
     1381 ggattgggtc aagaaaggtg ctattgtaat cgatgtagga attaaccgta ttgcaacaca
     1441 aacagat
//
LOCUS       DTVV01000445            2330 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2353718, whole genome shotgun sequence.
ACCESSION   DTVV01000445 DTVV01000000
VERSION     DTVV01000445.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2353718"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            15..752
                     /gene="panB"
                     /locus_tag="EYO86_05295"
     CDS             15..752
                     /gene="panB"
                     /locus_tag="EYO86_05295"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIC42479.1"
                     /translation="MRPISCLTAYTSSIAKIVDQNVDVILVGDSLGTVIYGFKNTRSV
                     TLEMMKNHGRAVMQSSQKSFTIIDMPYKTYVNKNQALDSAKHLLQFTGCQSVKLETDK
                     TTVPIVSHLIKNKILVVSHIGALPQHFKDFSKIRLIGKTTKQKKDMLDLAHQLEEAGS
                     SLLVLECIERELAKEITESISIPTIGIGSSINCDGQILVINDILNTDDTFKKPQFVKT
                     YANFSYYLNKAVKRYDTEVKNRKFPKK"
     gene            799..1656
                     /locus_tag="EYO86_05300"
     CDS             799..1656
                     /locus_tag="EYO86_05300"
                     /inference="COORDINATES: protein motif:HMM:PF02678.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIC42480.1"
                     /translation="MTRKVLNINDCMKTFEGDGIPVTRAFPVPEMREFDPFLLFDHFG
                     PINYEPGGATGVPAHPHCGFEAITYLLEGEVEHKDSWGGQATIRTGDVQWMTTGSGLV
                     HSELVTDKFKQSGGMMQGLQIWVNLPKKNKRVKPGYQHILKKNIPIIDTNDNIRIKVL
                     VGKVKETQSIIQTYSPVSIFDIQFKKEGKTNLDIPIEQIAMVYVIEGELQFAEANQIA
                     TEGQMIFFDQSGSEIHLTSISSSGSYLVLAGKSLNEPIARHGPFVANTEGEIKQAMSD
                     YQNGKMGQL"
     gene            1810..>2330
                     /locus_tag="EYO86_05305"
     CDS             1810..>2330
                     /locus_tag="EYO86_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC42481.1"
                     /translation="MKFFIIFCNVLLITILSSKSFASGEVNVYSYRQPILIDPFFEEF
                     TKSTGIKVNVLHAKKGLLERLLSEGANTPADLILTVDISRLKQFVEKDVLIPIDSSIL
                     KKNIPSHLRDSKNRWFALSKRARIVVVSKDRVPIGAITKIEDLAEPKWKGKICTRSGS
                     HAYNRSLLASIIAT"
BASE COUNT          840 a          349 c          426 g          715 t
ORIGIN      
        1 agcggctaaa aaatatacgg cctatttctt gcttaacagc atatacaagt agcattgcca
       61 aaattgttga tcaaaatgtt gatgttatat tggttggaga ctcccttgga acggttattt
      121 atggatttaa aaatacaaga tcggttactt tagagatgat gaaaaatcat ggaagagcag
      181 ttatgcaatc gtcccaaaaa tcatttacca taatcgacat gccctacaaa acatatgtaa
      241 acaaaaatca agcccttgat agtgctaaac acttattaca atttactggt tgtcagtcag
      301 taaaattgga aacggataaa acaacagttc ctattgtttc tcatttgata aaaaataaaa
      361 tattagtagt ttcgcatatt ggtgctttgc cccaacactt taaggatttc agcaaaatta
      421 gattaatagg caaaacgaca aaacaaaaaa aagatatgct tgatttagcg catcaactag
      481 aagaagctgg ttcaagtttg ttggtattgg aatgtatcga gagagagctt gcaaaagaaa
      541 ttacagagag cataagtata ccaactattg gcataggttc atcaattaat tgtgatggtc
      601 aaatattagt tattaatgac attttaaata ccgatgacac attcaaaaag ccacaatttg
      661 taaaaacata tgctaatttt tcttattatt tgaataaagc tgttaagcgc tatgatacag
      721 aagtaaaaaa cagaaagttt ccaaaaaaat aatttatggt ttttttgaaa aagttaatca
      781 gcattgagag ttgaaacgat gacgcgcaag gttcttaaca taaacgattg catgaaaaca
      841 tttgagggag atgggatacc tgtaaccaga gcttttcctg ttccagaaat gcgtgaattc
      901 gatccttttt tattgttcga tcattttgga cccattaatt atgaaccggg tggagcaacg
      961 ggtgtccctg cgcatccaca ctgtggtttt gaggccatta catatttatt ggaaggcgaa
     1021 gtggaacaca aggattcttg gggaggtcaa gctacaatta gaacagggga tgttcaatgg
     1081 atgaccacgg gatcaggttt ggtacactcg gaactagtga cagataaatt taagcaaagc
     1141 gggggaatga tgcagggttt gcaaatttgg gtcaatcttc caaaaaaaaa taaaagagta
     1201 aagcccggtt accagcacat tttaaaaaaa aacattccta tcattgatac aaatgacaat
     1261 atccggataa aagtactggt gggtaaggta aaagaaacac aatcgattat tcaaacgtac
     1321 tcgccagtgt caatatttga tattcaattc aagaaagagg ggaaaacaaa tttagacata
     1381 cctatagagc aaattgctat ggtatatgtt attgagggtg aattgcaatt tgctgaagca
     1441 aaccaaattg ccacagaagg tcagatgatt ttttttgatc aatctggttc cgaaatacac
     1501 ttaacatcca tttcttcatc aggatcctat cttgttttgg caggaaaatc tttgaatgaa
     1561 cccattgcta gacacggacc ttttgtagcc aatacagaag gagaaattaa acaagcgatg
     1621 tcagattatc aaaatgggaa aatgggccag ttataagatt tgtaattgtt gaaaagccac
     1681 cccttgattc aatgcctttt ttagaattat tataatttta aaaagtggtg tttttctttc
     1741 aaaagtaaca gaccaaataa ttttttttac tattacaaaa taactaataa taaaacaaaa
     1801 agaagtttta tgaagttttt tattattttt tgcaatgtat tacttattac aattctttct
     1861 tctaagtctt ttgcttcagg ggaagttaat gtctattcat atagacaacc tatattgatt
     1921 gatccatttt ttgaagaatt tactaaatca acaggaatca aagtaaatgt tttgcatgca
     1981 aaaaagggtt tattagaaag gttactatct gaaggtgcaa acacaccagc agatcttatt
     2041 ctaactgtag atatttcacg gttaaaacaa tttgttgaaa aagatgtttt gattcctatt
     2101 gattcttcta ttttgaaaaa aaatataccc tctcatttaa gagatagcaa aaatcgttgg
     2161 tttgctcttt caaaaagagc aagaatagta gttgtatcaa aagatagagt gccaattgga
     2221 gctataacta aaattgaaga tttagctgag ccaaaatgga aaggtaagat ttgtactaga
     2281 tcaggaagcc acgcctataa tagatcttta cttgcttcaa ttattgcaac
//
LOCUS       DTVV01000446            1017 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2356788, whole genome shotgun sequence.
ACCESSION   DTVV01000446 DTVV01000000
VERSION     DTVV01000446.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2356788"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            919..1017
                     /locus_tag="EYO86_05310"
                     /pseudo
     CDS             919..1017
                     /locus_tag="EYO86_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
BASE COUNT          337 a          174 c          198 g          308 t
ORIGIN      
        1 gcgacggcct ggttgaaacc cgtgcaaacc tgaaaaaaga tgacgcatcg gaggcttagg
       61 ggaatatcag aatatatgaa gattaagcca tttagatgtc taaaatgatc attttttggt
      121 catttatgga gaaattttgg gtattattgg cattaatcat caaataaaat gaaaaaatag
      181 cctttttacc ccttcataat taccattttt ctttattttt gcgtttataa tgccgtttag
      241 gccaaaatat aaagaaaaaa aactatcatt ttttcaccgc tgtgaataag taattttgct
      301 atggggctac gaatttgtgg cagattcatc tctccaaaaa gccggtttac tacccttcgt
      361 aaaataatcc gtgatcatat agggatcgat cttgtggctg ctcattttac ggatcagtaa
      421 atgtgaggga ttgccgaagt ccttgagggt cttttaagca ttgttaaatt tgtgcgatgc
      481 caagaaaggc ggtatagaaa tcaaatggtt cggttcccgc gcattctctt ggcatccctt
      541 atcccagcaa ttttaatggc tgcctgtagt acttcagaca gtaagcggaa tggccaaacg
      601 aacaccaatg cctatgtcta caatgccgaa gaattcaatc gagggtcgcc gacatttgcc
      661 aaggaaccca caaaaataga ttcagttaca atatgttata ataagtacgg gacaaatcct
      721 gcggtagtta taagtatggc cactgaagag tgccaaagat ttaacaaaaa agcgaaattt
      781 gttaggcagt ctttaagtgt ttgtccacta tttacacctg tggctgcgat ttacaattgt
      841 gtcgccggaa attaaaaaaa ttattaaaca caaacaatgc ttatgaagca gaaaatcata
      901 ttttatttca tgtgccatct tttaaaaatt aaaaattctt taaaggaggg ggttatattg
      961 accgatgtcg gatcggttaa agaaaatata atcaatttaa ttgaaaaaaa tatacca
//
LOCUS       DTVV01000447            1105 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2361512, whole genome shotgun sequence.
ACCESSION   DTVV01000447 DTVV01000000
VERSION     DTVV01000447.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2361512"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            60..1046
                     /gene="dusA"
                     /locus_tag="EYO86_05315"
     CDS             60..1046
                     /gene="dusA"
                     /locus_tag="EYO86_05315"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIC42482.1"
                     /translation="MNRKVSVAPMMDCTDRHDRFFLRLMSKNVMLYSEMVATKSAIHG
                     DRKKILSYSPDEKPLALQVGGSDKKELSEVAKIAEDMGYDEININLGCPSKKVQKNMF
                     GVCLMKEPDLVSECINEMVNSCKIPVTAKTRIGFDNTEDFDYLNKFIHKMKEAGCNTF
                     ILHARKAILTGLSPKQNLNIPKLNYDMVYKVKKENPDLEIIINGGISKINEIKNHLNS
                     CDGVMIGRSIYQNPYLLIDIEKEIFGTKNSLTREQVAERLLEYLDKEVKLGTKVNHIM
                     RHTVGLYYGQVGSKDWKRYLSDNMMARDSDFQKAKHIMTIVQNNEKVIQANT"
BASE COUNT          456 a          130 c          185 g          334 t
ORIGIN      
        1 aattgtaact tggctgccag ataaacttat gagtaaacct cttggatatt aaaaaagata
       61 taaatagaaa agtttcagta gcacctatga tggattgtac tgataggcat gaccgatttt
      121 ttcttcgact aatgagtaaa aatgttatgc tttattcaga aatggtagca acaaaatctg
      181 ctattcatgg agatagaaaa aaaatattat catacagccc agacgaaaaa cctttggctc
      241 ttcaagtagg tggatctgac aaaaaagaat tatctgaagt agctaaaata gctgaagata
      301 tgggttatga tgaaataaat attaatctag gctgtcctag taaaaaagtt caaaaaaata
      361 tgtttggcgt atgtttaatg aaagaaccag accttgtatc tgaatgtatt aatgaaatgg
      421 ttaattcttg taaaattcca gtaactgcaa aaacaagaat aggatttgat aatacagaag
      481 actttgatta tttaaataaa tttattcata aaatgaaaga agctggatgc aatactttta
      541 tattacatgc aagaaaagca atacttacag gactttctcc taaacaaaat ctaaacattc
      601 caaaattaaa ctacgatatg gtctacaaag ttaaaaagga aaatcctgat ttagaaatta
      661 taattaatgg tggtatttct aaaataaatg aaattaaaaa tcatttaaat tcatgtgatg
      721 gtgttatgat tggtagatca atttaccaaa acccgtattt attaattgat attgaaaaag
      781 aaatatttgg aacaaaaaat agtcttacga gagagcaagt agcagaaaga cttctagaat
      841 atttagataa agaagttaaa cttggaacta aagttaacca tataatgaga cataccgttg
      901 gcttatatta tggacaagtt ggttctaaag attggaagag atatttaagt gataatatga
      961 tggcaagaga ttcagatttt caaaaggcca aacatataat gactattgtt caaaataatg
     1021 aaaaagtgat tcaagctaac acataatgga aaatttaaac ttaaatgaaa tagttaatct
     1081 acttacagtg ttagcagttg cagca
//
LOCUS       DTVV01000448            1236 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2361876, whole genome shotgun sequence.
ACCESSION   DTVV01000448 DTVV01000000
VERSION     DTVV01000448.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2361876"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="EYO86_05320"
     CDS             complement(<1..408)
                     /locus_tag="EYO86_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD synthetase"
                     /protein_id="HIC42483.1"
                     /translation="MSSSIVSILYLISALLFIFSLRGLSHPDSSRVGNILGIIGMVIA
                     IITTLIFKNVLSYTEIGIAVLIGGFIGTIIALKIEMTALPQLIAAFHSLVGLAAVSVA
                     AAAFYDPESFNIGNIGTIPKSSLVEMSIGTFIGA"
     gene            complement(408..731)
                     /locus_tag="EYO86_05325"
     CDS             complement(408..731)
                     /locus_tag="EYO86_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HIC42484.1"
                     /translation="MDSIVTHGSDIFVFGLTVFLLACVIGYYVVWSVTPALHSPLMSV
                     TNAISSVIIVGAILATGPEGYGVSKIFGMVGVILASVNIFGGFIVTYRMLSMFKKKSK
                     AKKEK"
     gene            complement(733..>1236)
                     /locus_tag="EYO86_05330"
     CDS             complement(733..>1236)
                     /locus_tag="EYO86_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HIC42485.1"
                     /translation="LGAKFIMVEDEETQGAETSGGYAKEMSEEYKKKQSTLIAQTISK
                     QDIVICTALIPGKPAPVLVSEEMINTMARGSIVVDLAVEAGGNCPLSKLGQIVIHNGV
                     KIIGHANVPGRVAKDASNLYAKNIVNFLSLFIKKGEKKIDIDWKDEIIGAVVLSHNGE
                     LRLKEFK"
BASE COUNT          405 a          260 c          176 g          395 t
ORIGIN      
        1 agcacctata aaagtaccaa tactcatctc aacaagactg cttttaggga ttgttccgat
       61 attcccaata ttaaatgact ctggatcata aaaagccgct gcagcaacag acacagctgc
      121 tagaccaacc aaactgtgga atgcagcaat taattggggt aatgctgtca tttcaatttt
      181 aagagcaata attgttccaa taaaaccgcc aattaaaacg gcaataccaa tttcggtata
      241 acttaaaaca ttcttaaaaa taagcgttgt aatgattgct attaccatac cgataatacc
      301 taaaatattt cctacacgcg atgaatcagg atgtgataag cctctaagag aaaatataaa
      361 aagtaacgct gaaattaaat ataatattga tactatactc gatgacatta tttctctttt
      421 tttgccttag attttttttt gaacattgaa agcattctat aggttacaat aaatcctcca
      481 aaaatattca cagaggctaa aatgactcct accattccaa atatcttcga aactccgtaa
      541 ccttctggac ccgttgcaag gattgcgcca acaataatca cgcttgaaat agcatttgtc
      601 acgctcatta aagggctgtg cagagctggt gttactgacc aaactacata atacccgatg
      661 acacaagcaa gaagaaaaac tgtgagtcca aacacaaaaa tatctgagcc gtgggttact
      721 atactgtcca tcttatttaa attcctttaa tctcaattct ccattatgag ataatacaac
      781 agcaccaata atttcatctt tccagtcaat atcaattttt ttttcacctt ttttaatgaa
      841 taatgagaga aaatttacga tattttttgc gtataaatta gatgcatctt ttgcaacacg
      901 tccgggaaca tttgcatgac caataatttt tacaccatta tgaattacaa tttgacccaa
      961 tttgctaaga gggcaattgc cccctgcctc cacagcaaga tcaacaacta tagatccccg
     1021 agccattgtg tttatcattt cctcactcac taacaccggt gctggttttc ctggaataag
     1081 ggctgtgcaa attacaatat cttgttttga tattgtttga gcaattaatg tagattgttt
     1141 ttttttatat tcttcactca tttcttttgc atatccgcca ctcgtttccg caccttgtgt
     1201 ttcttcatct tccaccatta taaatttagc ccctaa
//
LOCUS       DTVV01000449            1279 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2362765, whole genome shotgun sequence.
ACCESSION   DTVV01000449 DTVV01000000
VERSION     DTVV01000449.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2362765"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="EYO86_05335"
     CDS             complement(<1..488)
                     /locus_tag="EYO86_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42486.1"
                     /translation="MRLGIKTDDEFLIKLNEKNIQIQNNFLEKIKEIAKKHSVNVMLQ
                     DGAVKKQETFDVEKIHQIYSDISERLETWTLEGISSTNDEGIRRNFIKLNINPGDHII
                     SLHLSIQYHVVLFYQPNYKVMKKQKELSDFMDKTKKQEGELTEKTDQVILEKLKAGGY
                     KK"
     gene            826..1206
                     /locus_tag="EYO86_05340"
     CDS             826..1206
                     /locus_tag="EYO86_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42487.1"
                     /translation="MDYFTTKRLFDTYGFTTDFSSLIPDFVRYPPSGNIPKGLMNTLA
                     LRGKLSTDNAALNPKLEDFTQMYHQMAAGLIDMSVDKLIPPGHPMHSKENSILLLKEE
                     NKKILKENLELKKKLENKLESRKI"
BASE COUNT          404 a          221 c          183 g          471 t
ORIGIN      
        1 aatttcttgt atcctccggc ctttaatttt tcaagtatta cctggtcagt tttttcagta
       61 agttcaccct cttgtttttt cgtcttatcc ataaaatctg ataattcttt ttgttttttc
      121 atcactttat aatttggctg ataaaataat accacatggt attgtattga aagatgtaat
      181 gaaattatgt ggtcacccgg attaatgttc aatttgataa aatttctccg aataccctca
      241 tcattagtgc ttgaaattcc ttccagtgtc catgtctcta atctttcaga tatatcactg
      301 taaatctgat gaattttctc tacgtcaaag gtttcctgtt tttttaccgc accatcctgc
      361 agcatgacat ttacagagtg ttttttcgca atttccttga ttttttcaag aaaattgttt
      421 tgaatctgta tatttttctc attgagtttg atcaaaaact cgtcgtctgt tttaatccct
      481 aatctcatat cttatcataa aatctgttca ataaaatcta ttcaaactgt aaatctgtca
      541 gggcattcca catgttcata gtgatgttcc gtaaactcat cctcaatgag atacattaca
      601 atcttatgta attccatagc ttcaatgtct tttccgcatt tgagacattt tcttttttcc
      661 ctgtatatca tttttttttc aaaggcatat gatcgctata agttaccgag acctagattt
      721 gctgatcagc gatcagctca agttgataaa agctaactgt gtttatgtca tatcattcca
      781 gttacgcgtg ataaagaaca aatatgccgg tttaaactaa aaaacatgga ttattttacg
      841 acaaaaagat tatttgatac atatggtttt acaacagatt tttctagtct cataccagat
      901 tttgtacgtt atccaccaag tggaaatatt cctaagggtt taatgaacac acttgcgtta
      961 agaggaaaac tctctactga taatgcagca ttgaatccaa agttggaaga ttttacacag
     1021 atgtatcatc agatggcagc aggccttatt gatatgtcag ttgataagtt aattccaccg
     1081 ggacacccaa tgcattcaaa agaaaattct atcttactac ttaaggaaga aaataaaaaa
     1141 attcttaagg aaaatttaga attgaaaaaa aaattggaaa ataaactaga atctagaaaa
     1201 atttaatata aataattaat tttatgcagg tgtgaatcct atgtatccgc ctgtttgtat
     1261 atttttttca gctgaaaaa
//
LOCUS       DTVV01000450            3121 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2369382, whole genome shotgun sequence.
ACCESSION   DTVV01000450 DTVV01000000
VERSION     DTVV01000450.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2369382"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="EYO86_05345"
     CDS             complement(<1..241)
                     /locus_tag="EYO86_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIC42488.1"
                     /translation="MSKRILIDSSDKDEIRVAVTTNGKLDDFEIETSKKTALKGDVYL
                     AKITRVEPSLQAAFVDFGVNRNGFLPLTEIHPDYFK"
     gene            387..2744
                     /locus_tag="EYO86_05350"
     CDS             387..2744
                     /locus_tag="EYO86_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HIC42489.1"
                     /translation="MNLMLNLLKKFLILGAFLIFICFSMLIYFLWKVSPELPSYNELK
                     SYNPSLTSRVFTSDGLLLDKYFIQERIFVPINRIPKNLIYAFISAEDKKFFEHIGIDP
                     LAIIRAVFTNIVNNFSNKKMIGASTITQQVVKNLLLTNEVSLERKFKEMILAVRIENI
                     LNKEQILELYLNDIYLGYGSYGIAAASLNYFNKSINELTLEEVAFLAALPKAPNNYNP
                     ITKYSNAIERRNWVLQRMYENGYISAAYLQYKNKPLIVQDRYEEQFEEANYFKEEVRK
                     KLYDLFGKQALYEQGLIVKTSINTKLQKIADHVLISGLINQDKKNGWKGVLANIQKLI
                     IEDDLLKNIKNPFPDKWAVYRINNISELILEVINSKGDHNTIDLSSDDNKWLIKEKFN
                     IGDVFFAELIADQLTIRQIPKINGAIVVIDPHTGKVLALSGGFSFELSEFNRATQAKR
                     QPGSAFKPFVYITAMKEGYTPATLILDAPYVVDQGPGLPKWKPSNYTDKFYGLSPMRT
                     GLEKSRNLMTIRLSDKIGMEKILDTARDFKIGKNMNNNLSMSLGSGLVTLLDLTNAYA
                     MIVNGGKEINPSLITSVYDKTGKQILINEIKKCNSCNQIDSEIDYSIPDIINNQQSVL
                     DPRIAYQITSMLEGVILRGTGRRIKELDTPLGGKTGTTNDNKDAWFVGFSPDLAVGVY
                     VGFDQPKSLGYKQTGSAVAVPIFKEFMKEAKVNPSKIPFRVPSGISFVKIDPKTGLVS
                     KISEGIFEPFIIGTEPYNQSRLKKLDNLGTINNNSISGTGSLLSN"
     gene            2832..>3121
                     /locus_tag="EYO86_05355"
     CDS             2832..>3121
                     /locus_tag="EYO86_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42490.1"
                     /translation="MEKDLLELKQKTNDPNIWENLEAKTLFQNIKIVEKKIADFHRIK
                     QSLSDLKEFYAFAVDENDQQTIIQLVEDSNQLLKDSTEVRYLNLMNGEVDQN"
BASE COUNT         1171 a          426 c          510 g         1014 t
ORIGIN      
        1 tcttgaaata atcagggtga atttcagtta aaggaagaaa accatttctg ttgacaccaa
       61 aatcgacgaa agccgcttgt aaagatggtt caactcttgt tattttagct aaataaacat
      121 cccctttgag ggcagttttt ttgcttgttt ctatttcaaa atcgtccagc ttcccatttg
      181 tagtaacagc gactctaatt tcatctttat ctgatgaatc gataagtatt ctttttgaca
      241 tatgattgaa actccgtaaa aaatattttt tgtattttgc attaaaattc tttatttttt
      301 gttattaaat tctattttga acaatttttt gaataagtag cctaatgtca tatttttaac
      361 tctttcacaa tctacaaatt atattgatga atttaatgct taatttactt aaaaaattct
      421 taatactagg ggcttttttg atatttatat gtttttcgat gctaatatat tttttatgga
      481 aggtttcccc tgaattacca agttataatg agttaaaaag ctataaccct agtttaactt
      541 ctcgggtgtt tacctctgat ggattgcttc tagataagta ttttattcaa gagagaatat
      601 ttgttccaat taatagaatt cctaaaaatt tgatttatgc attcatttct gcagaagata
      661 aaaaattttt tgaacatata ggaattgatc cactagccat tattcgtgcc gtatttacaa
      721 atattgtaaa taatttttcg aataaaaaaa tgataggagc ttcaaccatt actcaacaag
      781 ttgttaaaaa tttattgctt actaatgaag taagtttaga gagaaaattt aaggaaatga
      841 ttttggccgt ccgcattgaa aatattttaa ataaagaaca aatactagaa ttatatttaa
      901 atgatatata cttgggttat ggctcatatg gcattgctgc tgcaagctta aattatttta
      961 ataaatcaat aaatgaatta accctagaag aagttgcttt tttagcagct ttaccaaaag
     1021 cacctaataa ttataatcct ataacaaagt attcaaatgc tattgaaaga agaaattggg
     1081 ttttgcaaag gatgtatgaa aatggatata tatctgcggc atatttacaa tataaaaata
     1141 aaccacttat tgtacaagat agatatgaag agcagtttga agaagcaaat tattttaaag
     1201 aagaggtgcg aaaaaaactc tatgacttat ttggaaaaca agctttgtat gaacaaggct
     1261 tgatagttaa aacttcaata aataccaaat tacaaaaaat tgcagatcat gttttaattt
     1321 ctggacttat taatcaagat aaaaaaaatg gttggaaggg agtattagca aacatacaaa
     1381 aattaataat cgaagatgat ttattaaaaa atataaaaaa tccatttccc gataaatggg
     1441 ctgtttatcg aataaataat atatctgaat taatactaga agtaattaat tcgaaaggcg
     1501 atcataatac cattgatcta agttcagatg ataataagtg gctaataaaa gaaaaattta
     1561 atatcggaga tgtatttttt gcagaattga tagcagatca attgactatc agacaaatcc
     1621 caaaaatcaa tggtgctata gttgtaatcg atcctcatac agggaaagtt ttagctttat
     1681 caggaggatt tagttttgaa ttaagtgaat ttaatagggc aactcaagct aaaagacaac
     1741 ctggatccgc ttttaagcct tttgtttata taacagcaat gaaagagggt tatacccctg
     1801 ctactttgat attagatgcg ccttatgttg tagatcaggg acccggctta ccaaaatgga
     1861 aaccttcaaa ttatactgat aagttttatg ggttaagtcc aatgagaact gggttagaaa
     1921 aatcaagaaa tcttatgacg ataagattat ccgacaaaat aggtatggag aaaattttag
     1981 atactgccag agactttaaa attggaaaaa atatgaacaa taatttatca atgtccctag
     2041 gttctggatt ggttaccctt ttggatttaa caaatgctta tgcaatgatt gttaatggcg
     2101 gaaaagaaat taatccatca ttgattacga gtgtttatga taaaactgga aaacaaatat
     2161 taataaatga aattaaaaaa tgcaattcat gcaatcaaat agattctgaa attgattact
     2221 ccattcctga cattattaat aatcaacaat cagtattaga tccaagaata gcctatcaaa
     2281 ttacgtcaat gcttgagggt gtaattttga gagggacggg cagaagaata aaggaattgg
     2341 acactcctct tggaggaaaa acaggtacaa caaacgataa taaagatgct tggttcgtag
     2401 gtttttcacc agatttagca gtaggagtgt atgtaggctt tgatcaacca aaaagtttgg
     2461 gctacaaaca aacaggctcc gctgttgccg tcccaatttt taaagaattt atgaaagagg
     2521 cgaaagttaa tccaagtaaa attcccttcc gagttccttc aggaataagt tttgtcaaaa
     2581 ttgatcccaa gacgggtttg gtttcaaaaa tttctgaagg aatatttgaa ccttttataa
     2641 taggtactga gccttataat caaagcagac taaaaaaatt agataatctt ggaacaatta
     2701 ataataattc catttctgga acaggtagtt tattaagcaa ctaactatga cacaagaaga
     2761 tatagagaat aatattgaac aaatcactct caattttgat cttctaagga ggggagtttg
     2821 actatgacaa attggaaaaa gacttattag aattaaaaca aaaaactaac gatccaaata
     2881 tttgggaaaa cttagaagct aaaacacttt ttcaaaatat taaaattgta gaaaaaaaaa
     2941 ttgctgattt tcaccgtatc aagcaatcat tatccgattt gaaagaattt tatgcctttg
     3001 ctgttgatga aaacgatcaa caaacaatca ttcaattagt tgaagattcc aatcagctgc
     3061 ttaaggattc taccgaagtc aggtacttga atttaatgaa cggagaggta gaccagaaca
     3121 a
//
LOCUS       DTVV01000451            1581 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2369566, whole genome shotgun sequence.
ACCESSION   DTVV01000451 DTVV01000000
VERSION     DTVV01000451.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2369566"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(60..1343)
                     /locus_tag="EYO86_05360"
     CDS             complement(60..1343)
                     /locus_tag="EYO86_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HIC42491.1"
                     /translation="MPIQNFKPLATNKAKKNALQILEAGLTTAEPKNFLKFFINKNEI
                     HIGKSRIILSNYEKILVISYGKAADSMAKYVSEIVDVSQGIVVIPKYTKSCLTNKNFK
                     VFYSGHPVPNKESVRAGKAVLKFVNNRSNKDLIIFLISGGGSSLISVPDEITLSDKKH
                     VTKLLLQCGATINEFNCVRKHLSQIKGGKLVQKMNCDGCALIMSDVVNNDISVISSGC
                     TSADSTTSSDALKIIKKYSLEAKFPKTVISHLKKNLSKKTITQKNLRIKNKIIATNRN
                     CLDSMVSKSQVLGFKVIVSPIIQHDVVISAKRLVKMIPRNRRSCIIFGGEPTVNVKGK
                     GKGGRNQELVLQILKLIHHSNQNLIISSIGTDGIDGNTKYSGALIENNSYNPEEITHY
                     LKNNNSNLFFKKYGGLIKTGYTHTNLMDFGLILKY"
     gene            1423..>1581
                     /locus_tag="EYO86_05365"
     CDS             1423..>1581
                     /locus_tag="EYO86_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIC42492.1"
                     /translation="MHTVRIPKIIQFGKDAISEAEYPKNALIVTTAPPEISGRWLDKM
                     GIQDYMLYD"
BASE COUNT          514 a          239 c          239 g          589 t
ORIGIN      
        1 atacatacat tgatggaaaa atcatctgga aaaaagacaa agagtcttgg acataagcac
       61 tagtacttta aaatcaaacc aaaatccatt aaattagtat gcgtgtaacc agtttttatt
      121 aatccaccat attttttgaa aaataaatta gagttgttat ttttcaaata atgagtaatt
      181 tcttctggat tatacgaatt gttttcaatc aatgcaccag aatattttgt attaccgtcg
      241 attccatctg tcccaatcga agaaattatt aaattctgat tagagtgatg tattagtttt
      301 aggatttgta gaactaattc ttggttgcgc ccaccctttc cttttccctt tacattaact
      361 gttggctcac cgccaaagat tatgcaagat cttctatttc ttggaatcat ttttacaagt
      421 ctttttgcag aaattacaac atcatgttga attatagggc taactataac cttgaaccct
      481 aaaacttgtg atttggaaac catagagtct aaacaattcc ggttagttgc tatgatttta
      541 tttttaattc gtagattttt ttgagtaatt gtttttttgg acaaattttt ttttagatga
      601 gaaattacag tttttgggaa ttttgcttct agcgaatatt tttttatgat ttttagggca
      661 tcagaagagg ttgtgctatc agcagaagta catcctgatg aaattactga gatatcatta
      721 ttcacaacat ctgacattat cagtgcacag ccatcacagt tcattttttg aactagttta
      781 ccacccttaa tttgtgagag atgttttctt acgcaattaa attcattgat tgtagcacca
      841 cactgcaata gaagcttagt aacatgtttt ttgtcagaca gagtaatttc atcgggaaca
      901 gaaatcaagg atgaaccgcc gccggatatt aaaaaaataa taagatcctt gtttgaacga
      961 ttgttaacaa attttaatac cgcttttcca gctcttacac tttctttatt tggtacagga
     1021 tggcctgaat aaaatacctt gaaattttta ttagttaaac atgattttgt atattttgga
     1081 attacaacta tgccctgtga aacatctact atttcagaga catattttgc cattgaatct
     1141 gcagcctttc catatgaaat tactaaaatt ttttcataat ttgataaaat tatacgactt
     1201 tttccaatat gaatttcatt tttgttgata aaaaatttca aaaaattctt aggttctgcg
     1261 gtagttaatc cagcttctaa tatttgtaat gcattttttt ttgctttatt tgtagccaaa
     1321 ggtttaaaat tttgaattgg cattttgcaa gtttattgta aagaaattat taaatctata
     1381 cagatttgca agatataata ataatcatgt tttcaataat ttatgcatac agttaggata
     1441 ccaaaaatca ttcagtttgg taaagatgct atttctgaag cagaatatcc aaaaaatgct
     1501 ttgattgtaa caactgcccc ccctgaaata tctggtaggt ggttagacaa aatgggcata
     1561 caagactata tgttgtatga t
//
LOCUS       DTVV01000452            2407 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2370231, whole genome shotgun sequence.
ACCESSION   DTVV01000452 DTVV01000000
VERSION     DTVV01000452.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2370231"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(56..1351)
                     /locus_tag="EYO86_05370"
     CDS             complement(56..1351)
                     /locus_tag="EYO86_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIC42493.1"
                     /translation="MSIEVLILIFSFITLLLLNVPIAFCIGIPTTLVLVIGTGFTPAI
                     STVSQQIATAFDSFTLLAIPFFILSGNIMATGGIAKRLIDFAKTIVGGLPGGLALVNI
                     VSAMLFGAVSGSAIASAAAIGSFMHPRMIKDGYDPSYSAAVNVTSATTGLIIPPSNVL
                     IVYSLASGGVSIGALFVAGYLPGILVGVSLMIVASFLAYKQGFRSKPFKPFIEILKRF
                     FDALLGLTLIIIIIGGIIFGIFTATEAASIAVVYAFVLTIFIYKEIGIKDIPKIFLDT
                     AITTGVVMLLIGTSMSLSWVLSYANIPQNISIAMLGISENPLIILLIMNIILLMVGTF
                     MDMTPAILIFTPIFLPIATAMGIDPLHFGIMMVVNLCIGLCTPPVGTVLFVGCGVAKI
                     SISQIVKPLLPFYFIMVMVLILIIYFPGITLVLPQLLGM"
     gene            complement(1366..1872)
                     /locus_tag="EYO86_05375"
     CDS             complement(1366..1872)
                     /locus_tag="EYO86_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIC42494.1"
                     /translation="MKSITHFIDKILEKILILLFGLMVSVVTWQVFTRFLLNSPSSFT
                     EELSTYLLIWISLLGAVYALRKQAHLGIDIITSRLSGRAKEISTYIIHFFIILFSLFV
                     FMWGGIRLVWVTLQLNQISASLQIKIGYIYVILPITGLLMIYYSIYFLVNEKNKSFQT
                     TISKNYLN"
     gene            complement(1911..>2407)
                     /locus_tag="EYO86_05380"
     CDS             complement(1911..>2407)
                     /locus_tag="EYO86_05380"
                     /inference="COORDINATES: protein motif:HMM:PF03480.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIC42495.1"
                     /translation="EDLNGLKIRVMRSNLQIRTINLLGGNATPLAFSELYSALQQGVV
                     DGAENNPPNFYQQHFYDHCKYFVLDEHSSPPDVLVISTHTWNKFSSEQKQWLVKAVDE
                     SVIYQRKLWDEMTNWVYDEVKKSGVEIVHPEKRNFLKAVQPIYDDLDGTTIGEIAKEI
                     QNIE"
BASE COUNT          826 a          459 c          392 g          730 t
ORIGIN      
        1 accagtaaga tcaaacaagt tcattttgga tttcctctat taattgatgg aacatttaca
       61 tacccagcag ttggggtaac accaaagtaa tcccgggaaa ataaataatt agaattaata
      121 ccattaccat tatgaaatag aatggcagca atggtttaac gatttgtgat atcgatatct
      181 ttgctacacc acatcccaca aaaaggactg ttccgactgg cggtgtacaa agtccaatac
      241 aaagattaac caccatcatt attccaaaat gcaatggatc aatccccata gctgttgcta
      301 ttggtaagaa tattggagtg aaaatcagga tagccggagt catatccatg aacgtaccca
      361 ccatcaataa aatgatgttc ataatcagaa gaataatgag cggattttcg ctaatgccca
      421 gcattgcgat tgaaatattt tgaggaatat tggcatatga taatacccac gacagtgaca
      481 ttgatgttcc aattagtaac atcacaactc ctgttgtgat agcagtatcc aggaatattt
      541 ttggtatatc ttttatgcct atctccttat agataaatat tgtaagtacg aaggcatata
      601 caaccgcaat agacgcagct tcagtggcag taaagatacc gaaaattatt ccacctatta
      661 ttattattat gagtgttagg cctaataatg catcaaaaaa tcgttttaag atttcaataa
      721 atggtttaaa aggtttgctt ctgaacccct gtttatatgc taaaaaactg gcaacaatca
      781 ttagggagac tcccaccaat attcctggca aatatcctgc aacaaatagt gcacctatgg
      841 atactccacc acttgcaaga gaatacacaa ttaaaacatt gctgggcgga atgatcaaac
      901 ctgttgtcgc cgatgtaaca tttacggctg cactgtaact tggatcgtac ccatctttta
      961 tcattcttgg atgcatgaaa gagccaattg cagctgcaga ggcgatcgca gacccagaaa
     1021 cagccccaaa tagcatagca gaaacaatat tgaccaacgc taatccgcct ggtaatcctc
     1081 caacaattgt ttttgcaaag tcgattaagc gtttagcaat tccaccggta gccatgatgt
     1141 tacccgaaag aataaaaaac gggattgcca acaaagtaaa actatcaaat gctgtggcaa
     1201 tttgctgtga aacggtactt atagcaggtg taaatcctgt gccaataacc aatactaatg
     1261 ttgtaggaat cccaatacaa aatgcaattg gaacattcaa taatagcagt gtaataaaac
     1321 taaagattaa tattaatact tcaatactca tttcaaaacc tttaattaat tgagatagtt
     1381 ttttgaaata gtggtttgaa aacttttatt tttttcatta actaaaaaat aaatggaata
     1441 ataaatcata agtaatccgg ttatcggtaa aataacataa atgtatccaa tttttatttg
     1501 aagagatgca gaaatttggt ttagctgaag cgtcacccat actaagcgga ttcctcccca
     1561 cataaaaaca aataacgaaa acaaaataat aaaaaaatgt atgatgtagg tcgaaatctc
     1621 tttggccctt cctgacaacc tcgaggttat aatgtcaatt ccaaggtgtg cctgttttct
     1681 tagtgcatat acagctccca gtagactaat ccatattaag agataagtgg acaattcttc
     1741 tgtaaatgag cttggactat tcaataaaaa acgggtaaat acctgccatg taacaactga
     1801 aaccattaat ccaaacaaaa gaattaatat tttttctaat attttatcaa taaaatgtgt
     1861 aatgcttttc atgtgaactt ctttctaata taaaatttaa atagcttgta ttattctata
     1921 ttctgaattt ctttagctat ttctccaatt gtcgttccat ctaaatcgtc ataaatgggt
     1981 tgaacagctt taagaaagtt tcttttttct gggtggacaa tttctactcc tgatttctta
     2041 acttcatcat aaacccaatt agtcatttca tcccacaatt ttcgttgata aatcacggat
     2101 tcgtccactg ccttaaccaa ccattgcttc tgctcactac taaatttatt ccaggtgtga
     2161 gtactaataa ctaatacatc cgggggcgag gaatgttcat ctaatacaaa atatttgcaa
     2221 tgatcataga agtgctgctg ataaaaattc ggagggttat tctctgcacc atcgacaacg
     2281 ccttgttgta aagcagaata cagttcacta aatgctaaag gcgttgcgtt tcccccaagt
     2341 aagtttattg tacgaatctg aagattactt cgcatcaccc ttattttgag cccatttaga
     2401 tcttctg
//
LOCUS       DTVV01000453            3530 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2371992, whole genome shotgun sequence.
ACCESSION   DTVV01000453 DTVV01000000
VERSION     DTVV01000453.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2371992"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="EYO86_05385"
     CDS             <1..495
                     /locus_tag="EYO86_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011569823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC42496.1"
                     /translation="RFGPVARLARTLGWESPAFFTSPEMARFTIMVMDSWTYIPFMMI
                     MILAGMQSLPKEVLEAGKVDGAGKWSLFWKIIFPIMLPVSLTTIVIRIIFKLKLADII
                     ITVTAGGPGGATDSVTSFVFREYRDRSNVGYGTFLSFFYLICIIVFMTILIRFVNRWL
                     ERAA"
     gene            517..1392
                     /locus_tag="EYO86_05390"
     CDS             517..1392
                     /locus_tag="EYO86_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011569824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC42497.1"
                     /translation="MISKFSEKFLIYFLLILWTIICLFPIFWTATTSFKTAVNVTQGH
                     IIPWIDFTPAWKGWRSLGLSPDTIWQESTVREEFFNRFSNSFIISISASSLAALLGST
                     AAYGLSRFNYKFGPLRNPDISFFFLSQLILPPVVLALPVLILYKELDLFDTRVGVILL
                     YTLMVLPIVIWIMRDQFNAVPIELEEAALVDGSSVWGGYIKIVLPIVMPGIVAAFILS
                     LVLCWNEYFFAALITSTHAKTLPVMVASQTGSQGISWWTMAAISGASVAPLIIIGIIL
                     ERYIVKGMAAGAVKQ"
     gene            complement(1394..2878)
                     /locus_tag="EYO86_05395"
     CDS             complement(1394..2878)
                     /locus_tag="EYO86_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HIC42498.1"
                     /translation="MYLGVDIGTYETKGVLVKQDGKIISIASRKHKMIVPQKGWAEHR
                     PNEDWWGDFVYVCKKLIIKSNCNPEDIKAVATSAIGPCMLPVDNYGKALMNGVLYGVD
                     TRASKEIKYLNNKIGKENIFNFCGNALTSQSVGPKILWLKKNFPEIFSKTFKILNSTS
                     FIVYRLTGKYVIDHLTAVNFTPLYDIKGQCWSNELSNEIIDINLLPEILWSNEIAGEI
                     TKQASKETGLKLGTPVTTGTIDAAAEALSVGVDNSGDMMMMYGSTMFFYVLTDSKIFD
                     DRLWYAPWLFPNENCLSAGLATSGTLTHWFRDNFAKELQEETAFVDLAKEAEKSDIGS
                     KGLIFLPYFSGERTPIHDTHAKGIFFGLNLTHERKDLYRSIFEGIAYGTRHVFETYAE
                     IGNTPKEVFSVGGGLKNQIWSKTTSDITGANQIIRQITIGASYGDAFLAAVAIGDSNR
                     ENIHEWNPIEFEIKSEPNEIYEKGYKIFRNLYEHTKDIMKELDS"
     gene            complement(2887..>3530)
                     /locus_tag="EYO86_05400"
     CDS             complement(2887..>3530)
                     /locus_tag="EYO86_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIC42499.1"
                     /translation="GIKIGSPITYAPKAKKIGINKFAGTAVDDRAGCAVVIETAKKIK
                     NSKNRPTVHIVFSVQEEFNLRGALPVAEILKPDIAIQIDLILANDTPDMISKGNVKLG
                     GGPGMSLYSFHGRGTLNGVIPHPNLVKHFDKIANQLSIPLQRSANSGVLTDLSYVQLV
                     NGGVASIDVGFPLRYSHSSLEVCDLRDLDNLISLLESAIVKIDNNFILNRDNL"
BASE COUNT         1076 a          617 c          575 g         1262 t
ORIGIN      
        1 cgatttggcc cagtagcaag gttagcaaga actttgggct gggaatctcc agcctttttt
       61 acttcaccag agatggcgcg ttttacaata atggttatgg atagttggac ttacattcct
      121 tttatgatga tcatgatttt agcgggtatg caatctcttc caaaagaagt tttagaagct
      181 ggcaaagttg atggggctgg aaaatggtcg ttattttgga aaataatttt tcctattatg
      241 cttcctgtgt ctcttactac aatagttatt cgaataatat ttaagttaaa attagcggat
      301 attattatta ctgttacagc aggtggtcct ggaggagcaa ctgactctgt tacaagtttt
      361 gtttttagag aatatagaga tcgatcaaat gttggttatg gaacgttttt atcttttttt
      421 tacttaattt gcataattgt attcatgaca atattaatac gttttgttaa cagatggttg
      481 gagagggctg cctaatgaaa aaaaaggtaa tttaaaatga tttcgaaatt ttctgaaaaa
      541 tttttgattt attttctttt aatactatgg accattattt gtctttttcc tatattttgg
      601 acagcaacaa cttctttcaa aactgcagta aatgttacgc aaggacatat aattccttgg
      661 atagacttta cgccagcttg gaaaggatgg aggtctttag ggttatcacc agatacaata
      721 tggcaagagt ctaccgttag agaagagttt tttaatagat ttagtaattc ttttattatt
      781 tctataagtg cttcttcctt ggctgcatta ctaggttcaa ctgctgctta tggattaagt
      841 agatttaatt ataaatttgg tcctcttaga aatcctgata tatctttctt ttttttatct
      901 caattaatac ttcctccagt agtattagct cttcctgttt taattctgta caaagaattg
      961 gacttatttg atacaagagt tggcgtaatt cttctctaca cattaatggt tcttccaata
     1021 gtaatttgga tcatgcgtga tcaattcaat gcagtaccaa tagaactaga ggaagctgct
     1081 ctcgtcgatg gatcttctgt atggggggga tatataaaaa tagttttacc aattgtaatg
     1141 cctggaatag tagcagcatt tattttatca ttagtgttat gttggaatga atattttttt
     1201 gcagctctta taacaagcac acatgctaaa acattaccgg ttatggttgc atcacaaaca
     1261 ggctctcaag gtataagttg gtggactatg gctgctattt caggagcttc agttgctcct
     1321 cttataataa taggaataat tttagagcga tatatagtca aaggcatggc agccggggct
     1381 gtcaaacaat aattcagcta tcaagctctt tcattatatc ttttgtgtgt tcgtagagat
     1441 ttctaaaaat tttataacct ttttcataaa tttcatttgg ctcagattta atttcaaatt
     1501 caattgggtt ccattcatga atattttctc tattactatc gccaattgca acagctgcta
     1561 aaaaagcgtc tccataggaa gcgcctattg taatttgacg tattatctga tttgcaccag
     1621 tgatatcaga agtagttttt gaccatattt gattttttaa cccgccgcct acactgaaca
     1681 cttctttagg tgtatttcct atttctgcat atgtttcaaa tacgtgccta gtcccataag
     1741 ctattccttc aaatatcgat ctgtaaaggt cttttctttc gtgagtgaga ttaagaccaa
     1801 aaaatattcc tttagcatga gtgtcatgta ttggcgttcg ttctccggaa aagtaaggta
     1861 gaaaaattaa acctttggat ccgatatcag atttttcagc ttctttagct aaatcaacaa
     1921 aagctgtttc ttcttgaagt tcttttgcaa aattgtctcg aaaccaatgg gttaatgttc
     1981 cacttgtggc caaacccgca gataaacaat tttcatttgg aaataaccat ggagcgtacc
     2041 ataaacgatc atcaaaaatc tttgaatcag tcagaacata gaaaaacatg gttgatccat
     2101 acatcatcat catgtcccca gaattatcaa ctccaacact taaagcttct gccgcggcat
     2161 ctattgtacc tgttgttacg ggggttccta attttaatcc agtttctttc gatgcttgtt
     2221 ttgttatctc gcctgcaatt tcattagacc ataatatttc tggtaataag ttaatatcaa
     2281 taatttcatt gcttaattca ttactccaac attgaccttt gatatcataa agaggcgtaa
     2341 aatttactgc tgttaagtga tcaatcacat actttccagt aagacggtag acaataaaag
     2401 atgtcgagtt taaaattttg aaagttttag aaaaaatttc aggaaaattt ttttttaacc
     2461 atagtatttt tggacctact gattgtgatg taagagcatt accacaaaaa ttaaaaatat
     2521 tttctttacc aattttattg tttaaatatt taatttcttt agaggctctt gtatcaactc
     2581 catataaaac gccgttcatt agagcctttc catagttatc aactggtagc atgcaaggac
     2641 caatagcact tgttgcaact gcttttatat cttcaggatt gcaattagat ttaataatta
     2701 attttttaca aacatacaca aaatctcccc accaatcttc attggggcga tgttcagccc
     2761 accctttttg aggaacgatc attttgtgtt ttctagatgc tatagaaatt attttaccat
     2821 cttgtttaac taaaacacct tttgtttcgt atgtccctat atcaactccc aaatacagcc
     2881 gttgactcac aaattgtctc tatttaaaat aaaattatta tctattttaa caattgcact
     2941 ctctaataaa ctgattaaat tatctaaatc tcttaaatca caaacttcaa gagaagaatg
     3001 cgaatatctc aatggaaatc ctacatcaat agaggctact ccaccgttaa ctaactgaac
     3061 atacgataag tccgtaagta ccccagaatt agcacttctc tgcaatggaa ttgaaagttg
     3121 gtttgcaatt ttatcaaaat gtttaaccaa atttggatga ggtattacac cattcaatgt
     3181 tccccttcca tgaaaagaat aaagactcat tccagggcca ccacctagtt ttacatttcc
     3241 tttagagatc atatcaggag tatcattagc taaaattaaa tcaatttgga tagcaatgtc
     3301 aggctttaaa atctctgcaa ccggaagagc tcctcgaaga ttaaattctt cttgaacaga
     3361 aaaaacaatg tgtacagttg gtctattttt tgaattctta attttttttg cagtttcaat
     3421 gactactgca caaccagcac ggtcatctac agctgttcca gcaaacttat tgattcctat
     3481 tttctttgct ttaggtgcat aagttatggg cgaaccaatt tttattccaa
//
LOCUS       DTVV01000454            1901 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2378543, whole genome shotgun sequence.
ACCESSION   DTVV01000454 DTVV01000000
VERSION     DTVV01000454.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2378543"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            39..890
                     /gene="tilS"
                     /locus_tag="EYO86_05405"
     CDS             39..890
                     /gene="tilS"
                     /locus_tag="EYO86_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIC42500.1"
                     /translation="MLRKALVELEKNKGSLVALIVDHKIRKDSGKEAKTVCDYLKKHK
                     IRSKIINISKKNVLKKTMSEARQNRFLQMVRYCKKYKFLHLFFGHHNNDNIETFLLRK
                     IAGSNFEGLRSMQHKTIINGIQILRPLLTFNKLAIIRFNKSNNIEYIEDPSNDNLDYS
                     RVTVRKFLLQESTHINNIEKDFNLIQKYYPFYKQKLFQNFHKINTHTFNDEIVVNYKK
                     FAMIEKEIKIKIIEIIYKFLMPRRNILRYAKVIKALDLIEKKSSITTNLAGMHINKGN
                     VFISFML"
     gene            959..>1901
                     /locus_tag="EYO86_05410"
     CDS             959..>1901
                     /locus_tag="EYO86_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIC42501.1"
                     /translation="MKNITKNIVLWIIVGLLLVALFNLFQGTTSNKSYTKISFSDFII
                     ATDTGNVSEVNIRGNIIEGYFEDGRPFSTYSPNYPNLVDKLSSAGVKITAEPAGKSMH
                     PILSVLLSWFPMLLLIGVWIFFMRQMQGGGGKAMGFGKSKAKLLNEAVGKVTFDDVAG
                     IDEAKQELEEVVEFLKDQKKFSRLGGKIPKGALLIGPPGTGKTLLARAIAGEASVPFF
                     SISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQLLVEMDGFETNAGVIIIAATNRPDVLDTALLRP"
BASE COUNT          726 a          224 c          339 g          612 t
ORIGIN      
        1 aaagaaaaac gtataaggga acagttcaaa atcgaaactt gctgcgcaaa gcattggtag
       61 agctagaaaa aaataaggga tcattagttg cattaatagt cgatcataag atccgaaaag
      121 actcaggcaa ggaagctaaa acggtatgcg attacctgaa gaaacataaa attagaagca
      181 aaataataaa tattagtaaa aaaaatgttt taaaaaagac aatgagtgag gcgaggcaaa
      241 atcgtttttt gcaaatggtt agatattgca aaaaatataa atttctacac ctattttttg
      301 gacatcataa caatgataat attgaaacat ttttactcag aaaaattgca ggaagtaatt
      361 ttgaaggttt gagatctatg caacataaaa caattataaa tggtattcaa attttgagac
      421 ctttattaac atttaataaa ttggcaataa taagatttaa taaatctaat aatattgaat
      481 atattgaaga tcctagtaat gataatctag actatagtag agttactgta agaaaatttt
      541 tactacaaga atcaacccat attaacaata ttgaaaaaga ttttaattta attcaaaaat
      601 actatccatt ttataaacag aaattgtttc aaaattttca taaaataaat actcatacat
      661 ttaatgatga gatagttgtt aattataaaa aatttgcaat gatagaaaaa gaaattaaaa
      721 ttaaaattat agaaataata tataaatttt tgatgccaag aagaaatatc ttaagatatg
      781 ctaaggttat taaggcgctt gatttgattg agaaaaaaag ttctattact actaatcttg
      841 caggaatgca tattaataaa ggtaatgttt tcattagttt tatgctataa aacatcaaaa
      901 attatatttc ctattgtgtt tttacaatta attcctattt atattaaact aataaataat
      961 gaaaaatatt actaaaaaca tcgtcttatg gattattgtt gggttgctat tagttgcctt
     1021 atttaatctt tttcagggca ctacttctaa caaatcttat actaaaattt ctttctctga
     1081 ttttattata gctacagata caggcaatgt ttcagaagta aatatcagag gtaatataat
     1141 agaaggttat tttgaggatg gaaggccatt ttcaacatac tctccaaatt atcctaattt
     1201 agtagataag ttaagttcag ctggagtaaa aattactgcc gaacctgctg gtaaatctat
     1261 gcatcctata ttgagtgtat tactttcttg gtttcccatg cttttattaa ttggtgtgtg
     1321 gatttttttt atgcgtcaaa tgcaaggtgg tggggggaaa gcaatggggt ttggaaaatc
     1381 aaaagccaaa ttactaaatg aagctgttgg caaggttaca tttgatgatg ttgctggcat
     1441 tgatgaagct aaacaagaac ttgaagaagt tgtagagttt ttaaaagatc aaaaaaagtt
     1501 ttcacgtttg ggtgggaaaa taccaaaagg agctttatta ataggccctc caggaacggg
     1561 caagacattg cttgctcgag caattgcagg agaagcaagt gttcctttct tttctatatc
     1621 tggctctgat tttgttgaaa tgtttgttgg agtaggagct agtcgtgtga gagacatgtt
     1681 tgaacaaggt aaaaaaaatg caccttgtat tatttttatt gatgaaatag acgcagttgg
     1741 aagacataga ggtgctggat taggaggagg aaatgatgaa agagaacaaa cactcaatca
     1801 attattagtt gagatggatg gatttgaaac aaatgccggg gtaatcatta tagcagcaac
     1861 aaatagacca gatgttttag atacagcact gttaagacca g
//
LOCUS       DTVV01000455            2290 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2381955, whole genome shotgun sequence.
ACCESSION   DTVV01000455 DTVV01000000
VERSION     DTVV01000455.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2381955"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /gene="dnaK"
                     /locus_tag="EYO86_05415"
     CDS             complement(<1..1128)
                     /gene="dnaK"
                     /locus_tag="EYO86_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217336.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIC42502.1"
                     /translation="MSKILGIDLGTTNSCMAIVEASEGEVLENSEGGRTTPSVIAINP
                     KNDERYAGLMAKRQAVTNPENTIFSVKRFIGKKFSDSSLQKDMGMIPYKVKSGKNGDV
                     SVQMVGKDYTPQEVSSMILSKLKSDAETKLGEKITQAVITVPAYFNDSQRKATKDAGT
                     IAGLDVLRIINEPTAAALAYGLDKKTDETIAVYDLGGGTFDITILHIGDGVFEVKSTN
                     GDTHLGGDDFDQRIISWLVEDFKKEQGIDLMQDNMAIQRLREAAEKAKIELSTVMESE
                     INLPFITADASGPKHLVKTISRSHLENLIMDLVDKTIGPCKLAIKDAKLKTIDINEII
                     LVGGMTRMPAIQSAVEKFFGKEANKGVNPDEVVAVGAAIQAG"
     gene            complement(1142..1675)
                     /gene="grpE"
                     /locus_tag="EYO86_05420"
     CDS             complement(1142..1675)
                     /gene="grpE"
                     /locus_tag="EYO86_05420"
                     /inference="COORDINATES: protein motif:HMM:PF01025.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIC42503.1"
                     /translation="MTELKKTTPKKKTKNKAKNKNSEINKYKEEIKQLKNTALRSQAD
                     LINFKRRTLDDIQSNKTKIKTGIFTRFIITLDDLKLTLNSLPKGKQFKDWVTGMEIII
                     KNFEKSLNNEGLSEINPIKGDNFDPSKHESLSIENTNDSKLDGKISQTIKNGYSIENS
                     IIRPAQVILSNYIKKNS"
     gene            complement(1662..2036)
                     /locus_tag="EYO86_05425"
     CDS             complement(1662..2036)
                     /locus_tag="EYO86_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIC42504.1"
                     /translation="MEWDFFSEPNIEGVYVISVASRMLSMHPQTLRKYERIGLVRPSR
                     TGGMLRLYSQEDIAKLKLIKYLVDDLGLNLAGVQLILNIFNDLLDLKKGNEILKGMQL
                     KVFLENKLQKLFETLGLEYDRT"
     gene            complement(2128..>2290)
                     /locus_tag="EYO86_05430"
     CDS             complement(2128..>2290)
                     /locus_tag="EYO86_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HIC42505.1"
                     /translation="HGWPLRLVIPHLYGWKSAKWVKEIQFTSNPIPGFWEIRGYHMNG
                     DPWKQERFS"
BASE COUNT          642 a          401 c          310 g          937 t
ORIGIN      
        1 tcctgcttga atagcagctc caacagctac gacttcatca gggtttacgc ctttgttagc
       61 ttctttacca aagaattttt ccactgcact ttgtatagca ggcattctgg tcattccacc
      121 tacaagaatt atttcattta tatctatagt tttgagtttt gcatctttga tagctaattt
      181 acatggtcca attgttttat ctactaaatc cataattaaa ttttccaagt gagatcttga
      241 aatagtttta accaaatgtt ttggccctga agcatcagct gtaataaaag gaagatttat
      301 ttcactttcc ataacagttg aaagttcaat tttagctttt tcagcagctt cacgtaatct
      361 ttgaattgcc atgttgtctt gcattaaatc tatgccttgc tcttttttga aatcctcaac
      421 tagccaggat attattcttt gatcaaaatc atcaccgcca agatgggtat cgccatttgt
      481 agacttaact tcaaaaacgc catcacctat atgcaagatt gttatatcaa aagtacctcc
      541 acccaaatca tacactgcaa tagtttcatc agtttttttg tctaatccat atgctagagc
      601 agctgctgta ggttcgttaa ttattcttaa tacatctaat ccagctatag ttccggcatc
      661 ttttgttgct tttcgttgac tatcgttaaa ataagcagga actgtgatta cagcttgagt
      721 aattttttct cctaattttg tttcagcatc tgatttaagc ttagataata tcattgaaga
      781 tacttcttga ggcgtataat ctttgccgac catttgtact gaaacgtctc catttttacc
      841 tgatttaact ttataaggaa tcatccccat atctttttgt agcgaagaat cactaaattt
      901 ttttccaata aatcttttta cagaaaatat tgtgttttcc gggtttgtaa ctgcctgtct
      961 ttttgccatt agtccggcat atctctcatc attttttgga ttgattgcta ttactgacgg
     1021 cgttgtcctt ccaccttctg agttttctaa tacttctcct tctgaggctt cgactattgc
     1081 catacatgag tttgttgttc ctaaatcaat tcctagaatt tttgacattt tatgctccta
     1141 tttatgaatt cttttttata tagtttgata aaataacttg ggctggacgt attattgaat
     1201 tttctattga atatccattt ttaattgtct gagaaatttt tccatctaat ttagaatcat
     1261 ttgtattttc aatagaaagt gattcatgtt ttgatggatc aaaattatct cctttaattg
     1321 gattaatttc tgataaccct tcattattta aacttttttc aaaattttta attattattt
     1381 ccattcctgt cacccaatct ttaaattgtt tgccttttgg taaggaattt aaagtaagtt
     1441 taagatcatc aagagttatt ataaatcttg taaatatacc tgttttaatt ttggttttat
     1501 tagattgaat atcgtctaaa gttcttcttt taaaatttat taaatctgcc tgagaacgta
     1561 gagctgtatt tttcaactgt tttatttctt ctttatattt atttatttct gaatttttat
     1621 tttttgcctt attttttgtc tttttttttg gggtagtttt tttaagttct gtcatattca
     1681 agtcctaatg tttcaaataa tttttgcaat ttgttttcta aaaacacttt taattgcatt
     1741 ccttttaaaa tttcatttcc tttctttaaa tctaataaat cattaaatat atttaaaatt
     1801 aattggacac cagctaaatt aagacccaaa tcgtctacca gatattttat taattttaat
     1861 ttagcaatat cttcctgtga gtaaagtcta agcattccac ctgttctgct aggtctaact
     1921 aaaccaattc tttcatactt cctcaaagtt tgaggatgca ttgaaagcat tcgagatgca
     1981 acacttataa cataaacacc ttcaatattt ggttctgaga aaaaatccca ttccatgttg
     2041 tcaccaatat tgttataaca tgtattttat aggttgatac gaggtgtgtc aagtacattg
     2101 atagttttca ggtaagacta agtcttttta tgaaaacctt tcttgtttcc atggatctcc
     2161 attcatatgg taacctctta tttcccaaaa acccgggatt ggatttgaag tgaattgaat
     2221 ttcttttacc cattttgcac ttttccaacc atataagtgt gggattacaa gtcttaatgg
     2281 ccatccgtga
//
LOCUS       DTVV01000456            1806 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2384105, whole genome shotgun sequence.
ACCESSION   DTVV01000456 DTVV01000000
VERSION     DTVV01000456.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2384105"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="EYO86_05435"
     CDS             complement(<1..185)
                     /locus_tag="EYO86_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42506.1"
                     /translation="MNKNLSLAIEKAVVKINPKDNFSVNQKNVDKKPDLFSLTSQTEL
                     FQNEKGITIKIDRSKDS"
     gene            360..>1806
                     /locus_tag="EYO86_05440"
     CDS             360..>1806
                     /locus_tag="EYO86_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIC42507.1"
                     /translation="MPVFEDVHINPLGFREYDARWLYPKDINKSGIKNIGLGLGSQII
                     SKTNKSPRVIVGHDYRSYSEEVKTKLIEGLIDTGCNVEDIGLALSPTAYFAQFNLNAD
                     AVAMVTASHNENGWTGIKMGIERGLTHSPDEMNELKKIVLEKKFIKGKGSYKEIKGFK
                     EVYINNLIKNKIEKKLKVVVACGNGTAGVFAPKVLKEIGCEVVELDCNLDYNFPKYNP
                     NPEDIKMLHAISKAVKKNRADIGFGFDGDGDRIGIIDNNGKEIYSDKVGLLIARSLAP
                     NYKNKKFIVDVKSTGLFAKDKILLENNCKTIYWKTGHSHIKRKVNEVKALAGFEKSGH
                     FFFNQPLGFGYDDGINSAIQVCRLLENKDKKISEIINELPKTYQTPTMAPFCKDEEKY
                     QVVESLVKEVENLKANNTKIDNQKIADILTVNGVRFSLEDGSWGLIRASSNKPSLVIV
                     TESPTSDKRKKKIFEFIDEMVQKTGKVGDYDQ"
BASE COUNT          697 a          237 c          318 g          554 t
ORIGIN      
        1 ttcgaatctt tagatctatc gatttttatt gttatccctt tttcattttg aaaaagttct
       61 gtttgagatg ttaatgaaaa aagatcaggt tttttatcta catttttttg gttaaccgaa
      121 aaattgtcct tagggttaat ttttacgaca gctttctcga tggctagcga cagattttta
      181 ttcataaatc gtttgtttta ttagttcttt gtacaaaaac aactatatgt tgtgtgcaca
      241 atatcttgat atactacatg ttttaactaa tcaatagaac attatatgaa catagttata
      301 atagcaagga taaaatttaa atactgctaa aaatcaaaaa atattagata taaaggataa
      361 tgcctgtatt tgaggatgtt catattaacc cattaggatt tagggaatac gatgcacggt
      421 ggttgtatcc gaaagacata aataaaagtg ggatcaaaaa tataggtcta ggcttaggtt
      481 ctcaaattat ttcaaaaaca aataaatctc caagagtaat agtaggacat gattacagat
      541 catatagtga agaagtaaaa acaaaactta tagaaggctt aattgatacc ggatgtaacg
      601 tcgaagatat agggttagct ttatctccaa ccgcttattt tgcacaattc aatcttaatg
      661 ccgatgcagt agcaatggtc actgctagcc ataatgaaaa cggatggaca ggaataaaaa
      721 tgggaatcga aaggggatta actcactcac cagacgaaat gaatgaattg aaaaaaattg
      781 ttttggaaaa aaaatttata aaaggtaaag gttcgtacaa agaaattaaa ggatttaaag
      841 aagtttatat taataattta ataaaaaata aaattgaaaa aaagcttaaa gttgtagtag
      901 cgtgtggcaa tggtacagcg ggtgtatttg ctccaaaagt tcttaaagaa attggttgtg
      961 aagttgtaga gctagattgt aaccttgact ataactttcc caaatacaat ccaaatcctg
     1021 aagatataaa aatgcttcat gccatcagca aagctgttaa aaagaacagg gctgacattg
     1081 gttttggctt tgatggtgat ggggatcgaa ttggtataat agataacaat ggaaaagaaa
     1141 tttactctga caaggtaggt ttacttattg cgagaagctt agctccaaat tataaaaata
     1201 aaaaattcat tgttgatgta aaatcaactg gcttatttgc aaaagataaa attcttttag
     1261 aaaataattg caaaactatc tattggaaaa cgggtcattc tcatatcaaa aggaaagtca
     1321 acgaagttaa agccttagct ggatttgaaa aaagtggtca cttctttttt aaccaacctc
     1381 ttggttttgg atatgacgac ggaattaatt ctgcaattca agtttgccgt ttattagaga
     1441 ataaagataa aaaaatatca gaaataataa atgaactacc taaaacatat caaactccta
     1501 ccatggcacc tttttgcaaa gacgaagaaa aatatcaagt tgttgaatct ttggttaaag
     1561 aagtagaaaa tttaaaagcc aataacacca aaatagacaa tcaaaaaatt gctgatatat
     1621 taacagtaaa tggtgttcgt ttttctttag aagatggttc ctggggttta atcagagcgt
     1681 cttcaaataa accctcattg gtaattgtaa ctgaaagccc aacatccgat aaaagaaaaa
     1741 agaaaatatt tgaatttatt gatgaaatgg tacaaaaaac tggaaaagtt ggcgactacg
     1801 atcaaa
//
LOCUS       DTVV01000457            1258 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2386129, whole genome shotgun sequence.
ACCESSION   DTVV01000457 DTVV01000000
VERSION     DTVV01000457.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2386129"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1258
                     /locus_tag="EYO86_05445"
     CDS             <1..>1258
                     /locus_tag="EYO86_05445"
                     /inference="COORDINATES: protein motif:HMM:PF13231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42508.1"
                     /translation="PVLFASLESVLKDGLFSIKIISLLSGTGIVFFSYMVFKNIFSTK
                     IALVGQLFIAFNPWLGILSTSPLNDLLPIFMSILSFYFITKKDIKLTDIIFSASILGI
                     AFMFRAQPIVFLFAIIVFLIILEKKPRFKIFAVTLLIVFFVLCCSPMLLYNYTVHDKM
                     IDSNSNYYVAVHSKYYTQEWKDFLLDNMDKDANAIFSNPDLFSKNYFYNMFYGQPSNL
                     FGFDNKVSASLIPVIPIIGAIPVLGGLIYSLKIPASKKTLITVISTAAIATVLVFLLG
                     SFQDHFFAIIILPLIVLGLQNIHKVDKKFLLLIILAVIFSILMAVLPLRSYKHFLYLW
                     IFLAPLCSIFFVSVIPRLFLKIQNSKLSSTKILENQKSSKIIISVIISLILLANLSYS
                     YVTLEVITTGNSVDTILENFKSPFSEK"
BASE COUNT          367 a          202 c          174 g          515 t
ORIGIN      
        1 ccagttcttt ttgcgtcact tgaatctgtt ttaaaagatg gtttattttc aataaagata
       61 atctctcttc ttagtggtac aggcattgtg tttttttctt atatggtttt taaaaacatc
      121 tttagcacaa agatagcatt agtaggacag ctgtttattg cgtttaatcc ttggctaggt
      181 attctttcaa cttcaccatt aaatgatctt ttgccaatat tcatgtcaat tctctctttt
      241 tattttatca caaaaaaaga cataaaatta accgacatta ttttttcagc ctcaatttta
      301 ggtatagcgt ttatgtttag agcgcaacct atagtttttc tttttgcgat aattgttttc
      361 ttgataatac ttgaaaaaaa gcctagattc aaaatttttg ctgtaactct attaattgta
      421 ttttttgttt tgtgttgttc gcccatgtta ctctataact atacggtaca tgataagatg
      481 atagattcta attcgaatta ttatgttgca gttcattcaa aatattatac tcaagaatgg
      541 aaggacttcc ttcttgataa tatggataaa gatgccaatg ctattttttc taatcctgat
      601 ttattctcaa aaaactattt ctataacatg ttttatggac aacccagtaa tctttttggt
      661 tttgataaca aagttagtgc atcattaatt cctgtgattc ccattatagg agccataccg
      721 gtgttgggtg gattgatcta ttcattaaaa atcccagctt ccaaaaagac gctaatcacg
      781 gtaatatcta cagccgcaat tgctactgta ctggtttttc tgctgggaag ttttcaagat
      841 catttttttg ctattattat tctgcccttg atagttttag gtcttcaaaa tattcacaaa
      901 gtggataaaa aatttctttt gttaatcatt ttggctgtaa tcttttcaat attgatggcg
      961 gtgttgcctt tgcgttcgta taagcatttt ctttatttat ggatattttt agctccttta
     1021 tgttcaattt ttttcgtttc agtaatacct agattatttc ttaaaattca aaattcaaaa
     1081 ctttcttcta ctaaaattct tgaaaatcaa aaatctagca aaatcatcat atcagtaatt
     1141 atttctttga ttttacttgc taatcttagt tattcttatg taacgcttga agtcattaca
     1201 actggaaatt ctgttgacac catccttgaa aacttcaagt ctccattttc tgaaaaaa
//
LOCUS       DTVV01000458            1329 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2386703, whole genome shotgun sequence.
ACCESSION   DTVV01000458 DTVV01000000
VERSION     DTVV01000458.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2386703"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="EYO86_05450"
     CDS             <1..421
                     /locus_tag="EYO86_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIC42509.1"
                     /translation="GGRIISEKTKKRMKPFQKLVKRYGATAAFVAAATPIPDDIVYIP
                     LGLSKYSPKKFFIATLLGKLVLCYTLVLISHYMGLSILEPVLENIDDPLPVYIGIIIF
                     AAVMTITVILVLRLDWGRILGRIAPWTIDDDEDKNQN"
     gene            complement(418..975)
                     /locus_tag="EYO86_05455"
     CDS             complement(418..975)
                     /locus_tag="EYO86_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIC42510.1"
                     /translation="MRILSRKFYNRDTVQVAKDLLGKTLVRKIGKQKLTGTIIETEAY
                     KGKNDPASHASKKKTERNKVMFGQVGWSYVYFTYGMHYCFNVVAKKDGEESGAVLIRS
                     VQPRLGIGTMIKNRKVDLISNLVNGPAKLTQAMQITKKQYHVDLTKDSGLFIMDGIKP
                     TKILAKPRIGITVGADKLWNFSFKI"
     gene            complement(977..>1329)
                     /locus_tag="EYO86_05460"
     CDS             complement(977..>1329)
                     /locus_tag="EYO86_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIC42511.1"
                     /translation="VRNEVYITNIVKCRPPKNRAPNDTERMMCSDYLENELTIIKPKI
                     ICLMGNTAYHSILDGKEISKNHGRLIHKNNRVYFVTFHPAAIIYNQKLGKIFSADIKK
                     MVSILQKLNKKHRD"
BASE COUNT          393 a          222 c          203 g          511 t
ORIGIN      
        1 aggaggaaga atcatttctg aaaaaactaa aaaacgaatg aagccatttc aaaaattagt
       61 taagcgatat ggtgctacag ctgcttttgt tgcggcagca actccaattc ctgatgacat
      121 tgtttacatt ccattgggat tatcaaaata tagcccaaaa aaattcttta ttgcgacatt
      181 attagggaaa cttgttttgt gttacactct agtactaatt tcacattata tggggttatc
      241 aattttggaa ccagtacttg agaatattga tgatccgtta cctgtttaca taggaataat
      301 catatttgct gcggttatga caataacagt tattttggtt cttagattgg attggggaag
      361 aattctaggt aggattgcac catggacaat tgatgatgat gaagacaaga accaaaatta
      421 gattttaaaa ctaaaattcc atagtttatc agcacctact gtgattccaa ttctaggctt
      481 ggctagaatt tttgttggct ttataccatc cataatgaac aagcctgagt cttttgttaa
      541 atctacatga tattgttttt tagtaatctg cattgcctgt gttaattttg caggtccatt
      601 tactaaattt gagatgagat ctacttttct attttttatc atagttccaa ttcctaatcg
      661 tggttgaact gagcgtatta aaaccgcacc agattcttca ccatcttttt ttgcaacaac
      721 attaaagcaa tagtgcatgc cataggtgaa ataaacatat gaccacccaa cttgaccaaa
      781 cattaccttg tttctttcag tttttttctt agatgcatga ctagcgggat cattttttcc
      841 cttgtatgct tctgtttcta tgatggtacc agttaatttt tgttttccga tttttcttac
      901 caatgtttta cccaagagat cttttgcaac ttgcacagta tctctattat aaaattttct
      961 agacaaaatt ctcattttaa tctctatgtt ttttatttaa tttttgtagt atacttacca
     1021 tctttttaat gtcagcacta aaaatttttc caagtttttg attgtaaatt attgctgctg
     1081 gatggaaagt aacaaaatat acacgattat ttttatggat taatctacca tgattttttg
     1141 aaatttcttt tccatctaaa attgaatgat aagcggtatt tcccattaaa caaattattt
     1201 ttggcttgat aattgttagc tcattttcta ggtaatcact acacatcatt ctttctgtat
     1261 cattgggcgc cctatttttt ggcggtctgc atttgacaat atttgtaata tatacctcat
     1321 tccttacca
//
LOCUS       DTVV01000459            1354 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2392938, whole genome shotgun sequence.
ACCESSION   DTVV01000459 DTVV01000000
VERSION     DTVV01000459.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2392938"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="EYO86_05465"
     CDS             <1..187
                     /locus_tag="EYO86_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIC42512.1"
                     /translation="KIKYEKIVAKILAVSKNRTDTRIYVRGDKTINYGRVLEIIGMLS
                     GSGFTKVALISEPYKER"
     gene            192..1004
                     /locus_tag="EYO86_05470"
     CDS             192..1004
                     /locus_tag="EYO86_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HIC42513.1"
                     /translation="MYRNLAISTGFHLLIIIVATLSLPFLAKTPIELPPIISIELIQI
                     TDKTNIPFAPKAKKIIEKVKKREKERVLSEQAPPKKIKKEKPDAVPLPDKKIEKVKKI
                     EEKKQNPKKIETEVKQVSEFEKKELFDPNNIAALIDKSKEDLAETTKTTNKITQSQDR
                     NAEPSGLSLSEEDALKAQIFGCWSIPLGLPYNKNLLVRIKMQLKPDGTVIKSEILDHA
                     RMNKPGQGFYKVLAESALRAIRLCQPLRVPTTGYERWKDLQLNFDAREMLEG"
     gene            1009..>1354
                     /gene="tolB"
                     /locus_tag="EYO86_05475"
     CDS             1009..>1354
                     /gene="tolB"
                     /locus_tag="EYO86_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953642.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HIC42514.1"
                     /translation="MNLLRILTILLLIQFKAYALIEVDITRGNLSPLPIAVSALFSDN
                     DSHAKFEKILKQKDLGSEISIIIENNLKQTGLFNPLDKDAFLQNSEIAHLKPRVEDWA
                     LIKSQALITGKVE"
BASE COUNT          577 a          184 c          221 g          372 t
ORIGIN      
        1 aaagataaaa tatgaaaaaa ttgttgcaaa aatattagct gtttcaaaaa atagaacaga
       61 tactagaatt tacgtaagag gagacaaaac aattaattat ggaagagttt tagaaataat
      121 aggcatgctg tctggttcag gatttactaa agttgctcta atttcagaac cttataaaga
      181 aaggtaagta agtgtataga aatcttgcta tttcaacagg ttttcattta cttataatta
      241 ttgtagcaac tttgagtttg ccttttttag caaagacgcc aattgaactt ccacctatca
      301 tatctataga actaattcaa attactgaca aaacaaacat tccatttgct ccaaaagcaa
      361 aaaaaataat agaaaaagtt aaaaaaagag aaaaagaaag agtgctttct gaacaagcac
      421 cgccaaaaaa aataaaaaaa gaaaaacctg atgctgtgcc tttaccagat aaaaaaattg
      481 aaaaagttaa gaaaatagaa gaaaaaaaac aaaatccgaa gaaaatagaa acagaagtta
      541 aacaagtttc agaatttgaa aaaaaggaat tgtttgatcc aaataacatt gctgcattaa
      601 ttgataaatc caaagaggat cttgctgaaa ctacgaaaac cacaaacaaa attacacaat
      661 ctcaagatag aaacgcagaa ccaagcggac tttctttaag tgaagaggac gcattaaagg
      721 cacaaatttt tggatgttgg agtattccgt tgggattgcc atataataaa aatttgcttg
      781 ttcgtataaa aatgcaattg aaaccagatg gaacagttat aaaatcagag atattagatc
      841 atgctagaat gaataagcct ggacaaggat tttataaagt gttagcagaa agtgcgttaa
      901 gagctataag attatgtcag ccattaagag taccaacaac aggatacgaa agatggaaag
      961 acttacaact gaattttgat gccagagaga tgttagaagg ataataattt gaatttatta
     1021 agaatattaa caatactgtt attaattcaa ttcaaggctt atgctttaat tgaagtagat
     1081 ataactagag gaaatttgag ccctcttcca atagcagtgt cagcattatt ttcagacaat
     1141 gactcacatg caaaatttga aaaaattttg aaacaaaaag atcttggttc tgaaatatcc
     1201 atcatcattg aaaataattt aaaacaaact ggacttttta atcctcttga caaagatgca
     1261 tttttacaaa actcagaaat tgcccattta aaaccaagag ttgaagattg ggctttgatt
     1321 aaatctcaag cattaataac tggaaaagta gaaa
//
LOCUS       DTVV01000460            1390 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2395634, whole genome shotgun sequence.
ACCESSION   DTVV01000460 DTVV01000000
VERSION     DTVV01000460.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2395634"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            8..1000
                     /locus_tag="EYO86_05480"
     CDS             8..1000
                     /locus_tag="EYO86_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020169551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIC42515.1"
                     /translation="MIFRQFFDQISCTYTYLIASDKGREALIIDPVLEKTDQYIKLLN
                     ELELKLVKVIDTHIHADHISGLAELRNRTKCITLMGEHAPADVVSMKVKDNENIHIEN
                     IQLTSIYTPGHTDDSYSFLMNDRVFTGDTLLIKGTGRTDFQNGNPYDQYHSLFDRLLK
                     LPKNTFIYPAHDYKGENVSTIGEEINFNPRLQVTSVQEYVKIMENLNLDNPKLMDVAV
                     PANQSMGMTNETQKMLNGISYDKLINLVKNDQSILIDLREDIEIDKKPSLDNSINITY
                     SSLQYYLNENKDLLKSKTLIFYCAVGERSALAIQICQAYNLNNNYHLIGGINNL"
     gene            1049..>1390
                     /locus_tag="EYO86_05485"
     CDS             1049..>1390
                     /locus_tag="EYO86_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC42516.1"
                     /translation="MLNRNLIILIFSQIFSFTVAPVTVLLSGIIASGMIDIKYIATLP
                     TALTVVGTAIGSVFASYLMSIKGRKFGFIFSSLMSSFAALLASFAVFNDYFIIYCLAN
                     LILGLGLAFTAQ"
BASE COUNT          480 a          204 c          218 g          488 t
ORIGIN      
        1 atatataatg atttttagac agttttttga ccaaatttct tgcacttaca catatttaat
       61 agcaagtgat aaaggtagag aggccttgat tatcgatcct gttttagaaa aaacagatca
      121 atatattaaa cttttaaatg aacttgaatt aaagttagtg aaagtgatag atacgcatat
      181 tcatgcggat catatttcag gtttggcaga attgagaaat cggacaaaat gcatcacatt
      241 aatgggtgaa catgcgccag cggatgttgt gtcaatgaaa gtaaaggata atgaaaatat
      301 tcatattgag aatattcaat taacctcaat ctacactcct ggtcacacag atgattcata
      361 tagtttttta atgaatgatc gagttttcac cggagacact cttttaatca aaggtacggg
      421 aagaacagat ttccaaaatg gcaatcccta tgatcaatat cattcattat ttgatcgatt
      481 attaaaatta ccaaagaata cattcattta ccctgcgcat gattataaag gtgagaatgt
      541 aagcacaatt ggagaggaaa ttaattttaa tcctcgcctt caagttactt cagttcaaga
      601 atatgtaaaa attatggaaa atttaaactt agataatcca aaattaatgg atgttgctgt
      661 gcccgcaaat caatccatgg gaatgacaaa tgaaactcaa aaaatgctga acggaatttc
      721 ttatgacaaa ttaatcaatc ttgtaaaaaa tgatcaatca atattaattg atctaagaga
      781 agatattgag attgacaaaa aaccatcatt ggataattca attaatataa cttactcttc
      841 attacaatat tatttgaatg aaaacaaaga tcttcttaaa tccaaaactt taatctttta
      901 ttgtgcggta ggggagaggt ctgccttagc tattcaaatt tgccaagcgt ataatttaaa
      961 taataattat cacttaatag gtggcattaa taatctttaa tcgttaatag cgttctagtt
     1021 ttatatttta cattttcaaa tcattcttat gcttaatcgc aatttaatca ttcttatatt
     1081 ttcccaaatt ttttcattta cagttgcgcc tgtgaccgtt cttttaagtg gaattatcgc
     1141 atctggaatg attgatatta aatatattgc gacattgcca acagctttaa ccgttgttgg
     1201 tacagcgata ggaagtgttt tcgcctctta tttaatgagt attaaaggtc gaaaattcgg
     1261 atttattttt tcttccttaa tgagttcatt tgccgcttta ttagcttcat tcgcagtatt
     1321 taatgattat tttataattt attgtttggc aaatttaatc cttggattag gtcttgcatt
     1381 tacggcacaa
//
LOCUS       DTVV01000461            1422 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2395792, whole genome shotgun sequence.
ACCESSION   DTVV01000461 DTVV01000000
VERSION     DTVV01000461.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2395792"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            106..1161
                     /locus_tag="EYO86_05490"
     CDS             106..1161
                     /locus_tag="EYO86_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIC42517.1"
                     /translation="MRLLIGGSSSFFFHLKEFSDTLNRLGVESKLVFDADYTDGFPSR
                     KIRNWFQTGKKFTKLIDEFKPDAIFVDRQRHFGVAALKSNIPLFVLLRGHYWSELYWN
                     KRTMYKTFHKRIALLQWDKFAKEIFNGATAILPICRYLEKVANEYLPHKATDVFFEGI
                     TASRWYQVKGMKLKHPCVGLLQRSNWWGKTSEMLILKKVLEKMPDVNFYWAGDGPLRD
                     RILSELKRYDNFHWLGSLEHPDKVREYLSEIDVYALITGMDLAALSLKEAQLMKKPVV
                     ATNVGGNPEMMINGKTGFLVEKGNSKQLIEKLSLLLEDGDLAKKMGNEGRKFVEETFN
                     WELITKNFIKIAKSYLK"
     gene            complement(1168..>1422)
                     /locus_tag="EYO86_05495"
     CDS             complement(1168..>1422)
                     /locus_tag="EYO86_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011013024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIC42518.1"
                     /translation="FFLKVPVVGTDVGGIPELIKNNETGLLVPPNNPQKLAESVNKLL
                     ENKQNANQLANSGYKFIKNNMTWDIILPKYIEFYENLLKN"
BASE COUNT          512 a          176 c          260 g          474 t
ORIGIN      
        1 ggttgattga agttatttca tctaaaaatt tcgaaagcat ttagtatatt ttggtcgatt
       61 atctattata attaagaaaa atctaatact aacgaaggga aaaatttgag attactaata
      121 ggcggttcaa gttcattttt ttttcacttg aaagaatttt ctgacacatt aaataggtta
      181 ggggttgaat ctaaattagt ttttgacgct gattatactg atggatttcc aagtagaaaa
      241 ataagaaatt ggtttcaaac tggaaaaaaa ttcactaaat taatagatga attcaaacca
      301 gacgcaattt ttgttgatag gcaacgacat tttggagttg ctgcactaaa atcgaacatt
      361 ccactgtttg ttcttctacg aggtcattat tggtcagaat tgtattggaa caaaagaaca
      421 atgtacaaga catttcataa acgaattgca ttattacaat gggataaatt tgctaaagaa
      481 atttttaatg gcgcgactgc aattttacca atttgtagat atctagaaaa agttgcgaat
      541 gaatatttac ctcataaagc aactgatgtt ttttttgaag gaataactgc atctagatgg
      601 taccaagtaa aaggaatgaa gctaaaacat ccatgtgtag gtttgttaca gcgttcaaat
      661 tggtggggaa agacttcaga aatgctaatt ttgaaaaaag tattagagaa aatgccagat
      721 gttaattttt attgggcggg cgatggacct cttcgagata gaatactttc tgaactaaag
      781 aggtatgata attttcattg gttaggaagt ttagaacatc cagataaagt aagagaatat
      841 cttagtgaga ttgatgttta tgcgttaatt acaggaatgg atttagctgc gttatctcta
      901 aaagaagcac agttaatgaa aaaacctgtt gtcgcaacca atgttggagg aaatcctgaa
      961 atgatgatta atggcaaaac tggatttttg gtagaaaaag gcaattctaa gcagttaatt
     1021 gaaaaattat ctttattgct tgaagatggt gatttggcaa aaaaaatggg aaacgaaggt
     1081 cgtaaatttg ttgaagagac atttaactgg gaactaatca caaaaaattt tattaaaata
     1141 gcaaaatcat acttgaaatg aatatgatca attctttaga agattttcat aaaattcaat
     1201 atactttggt aatataatat cccatgtcat attatttttt atgaatttat atcccgagtt
     1261 tgcaagttga ttagcatttt gtttattttc taacaatttg ttaacagatt cagccagctt
     1321 ctgtggattg tttggaggaa ctaataatcc agtttcatta ttttttatta gttctggaat
     1381 acccccaaca tcagttccaa caacaggaac tttcaagaaa aa
//
LOCUS       DTVV01000462            1639 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2400836, whole genome shotgun sequence.
ACCESSION   DTVV01000462 DTVV01000000
VERSION     DTVV01000462.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2400836"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="EYO86_05500"
     CDS             complement(<1..672)
                     /locus_tag="EYO86_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIC42519.1"
                     /translation="MQNKSYLKCSIPKCGKEYPITSTEFKCVCGNLLDVKYKSNPPTN
                     LKDVFYQRRNPQGSIFNESGVWRFRELLNFCAIETNDLAQCSKHLVSLDGAEGRQSKP
                     YHMSKVSKFIGIENEKLMLQPEGYNPSGSFKDNGMSAAVTHAKMVQAKKIICASTGNT
                     SASAGMFAANENMDCDVYIPEGEIAPGKLSQAYQFGTQLIQVKGNFDDAFTKSLEVTS
                     QSNGYT"
     gene            761..1159
                     /locus_tag="EYO86_05505"
     CDS             761..1159
                     /locus_tag="EYO86_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42520.1"
                     /translation="MPRILEIVLIDFNEYLKGILQQILASYKILTELNDNPSDLHTMK
                     QEISKIIGLSLVVKNKLEGKKNQSDSFVTIYKLFSYYIETYDFSREIDILAQIYYKDS
                     NRLKNLRLLIIDSLNDKHLIEKLQKILNEL"
BASE COUNT          549 a          254 c          230 g          606 t
ORIGIN      
        1 tgtataacca tttgattgac tggttacctc taatgatttt gtaaacgcat catcaaaatt
       61 tcctttaact tgtattaact gtgtgccaaa ttgatacgct tgactgagct tacccggtgc
      121 aatttcccct tctggtatat acacatcaca atccatattc tcgtttgctg caaacatacc
      181 agctgaagca gatgtgttgc cagtagatgc acaaataatt tttttagcct gaaccatttt
      241 tgcatgtgta acagcagcag acattccatt gtctttgaat gaaccacttg ggttgtatcc
      301 ttctggttgc agcattaact tttcattttc aataccaata aactttgaaa cctttgacat
      361 atggtatggt tttgattgtc taccctctgc cccatcaagt gaaactaaat gttttgaaca
      421 ttgagcgaga tcattagttt ctatcgcaca aaaattcaaa agttcacgaa atcgccaaac
      481 tccactttca ttaaatatac taccctgcgg atttcttcgt tggtaaaaaa cgtccttaag
      541 atttgtcggt ggattgcttt tatacttaac atctaaaagg tttccacata cacatttgaa
      601 ttcggtagat gtaataggat attctttacc acatttagga atcgagcatt ttaaatatga
      661 tttattttgc aacgtgacaa tatatttact gactaattta aagataagaa aactgacaaa
      721 tttttttgct attttaactt aatattacta aactttttga atgcctagga tattagaaat
      781 tgttttgata gattttaatg aatatttaaa agggatttta cagcaaatac tggcgtctta
      841 taaaatacta actgaattaa atgataatcc gagtgacctt catactatga aacaggaaat
      901 atctaaaata attggattat ctctggtagt aaaaaataaa ctggaaggaa aaaaaaatca
      961 gtctgacagt tttgtaacca tttacaaatt attttcatat tacattgaaa cttatgactt
     1021 ttcaagagaa attgatattt tagcacaaat ttattacaag gattctaata ggttaaaaaa
     1081 tctaaggctg ttaataattg attcattaaa tgataaacat ttgattgaaa aactccaaaa
     1141 aatattaaat gaactataat tttttcatga tgaaatttta tttttttgtg ttttaggtcc
     1201 agatcttaaa tgaaaattac tacagcattt gcactcattt ttaacacatt cattttcgtc
     1261 aaaatgtcca caaataccac attcacaatg tgtaaccatt agaaacatta tacggtgtag
     1321 aatataacta aaacgtaaaa tgttatttga aataagcaat ttgccgcaaa attttcaatt
     1381 ataaaaaata aaaaatttat tttttatgtc tagatttttt ggttgttttt ccccctattt
     1441 ttttacttga gatatttttt atttgtgcag ttttatcatc aactgtttta attatttctt
     1501 gaattaaact gtctaaatgt gtatcatcag cctttggttc aatcgcagta gagatttcct
     1561 gtatgacatt tgaaatttct gtgctgagtt gttcaaaact ttcaggatcc tcatatgctg
     1621 tgttataaag ggattttaa
//
LOCUS       DTVV01000463            4834 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2402670, whole genome shotgun sequence.
ACCESSION   DTVV01000463 DTVV01000000
VERSION     DTVV01000463.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2402670"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            45..413
                     /locus_tag="EYO86_05510"
     CDS             45..413
                     /locus_tag="EYO86_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42521.1"
                     /translation="MLNTLMELINNKAVLSSRVVTGGGLVIALSKMSFMNEVGILSTM
                     GEESFPEKLFNESLSIVVQIYNRDVGAAQKTLETNNIPFDIIGITTPEKTIKINDRVN
                     LLIKDAKNIWENSLRKKLLS"
     gene            377..1453
                     /locus_tag="EYO86_05515"
     CDS             377..1453
                     /locus_tag="EYO86_05515"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:PRK05385.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIC42522.1"
                     /translation="MGKLIEKKTIIMKQKISYKDAGVDIEKTYRAIASSKKTIQATFT
                     KNVLSSIGGFGSMYSLKDILKNYDEPIMVQSIDGVGTKMSVAQQCKNFNFVGVDLVNA
                     CCNDVAATGAKPITFLDYLASSILDPSAISEIIKSIAGECKRLGVSLVGGETAEMPGT
                     YHKNEYDLVGVATGIIDKKNIIDGSKIKNGSTILGLPSSGLHTNGYSLARKLIFETLR
                     LDTNDTLPNQNITIERALLAPHKNYSTVINGLVSDRQNIEGIAHITGGGLIDNIPRIL
                     PNGLAAEIDINTWKKIPIFEFLTNNLDLNTKELYRTFNMGIGLILIVEANQKGSVMKT
                     LENDGCIEVGRIITSPSKEVYLSE"
     gene            1446..2702
                     /locus_tag="EYO86_05520"
     CDS             1446..2702
                     /locus_tag="EYO86_05520"
                     /inference="COORDINATES: protein motif:HMM:PF13507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HIC42523.1"
                     /translation="MNNKPKIAFIQFPGSNTENETIATIERNNMVAVPHFWNNPFSQL
                     RACDGYIILGGFSYEDRSRSGIIASLDPIMEEVKKAIVLGKPVLGICNGAQILVESGI
                     VPGNESFGTIASLTQNKRVQNGQIVGTGYFNTWCHIKPNQKSRSAFIDKNNGGVLSVP
                     LAHAEGRFMMSKKLSSIIKENNLATYRYCTESGELTSDFPTNPNGSVDNVAALGNLPG
                     NAMAIMPHPERTIGGGGDAVFKAMHKYIVQKGAYSYRALKYAAEKITVSPFEKSPTTK
                     EIIISTIIADNEASSVEKCVQKLGADTTVKRYVHFEIEADKNMDLKDVYKTNVLFNPS
                     KEFMVNLDNTNKTKRFLVREKENIEGRRIKEILQKRFGFDKIKNIQKSVVWEISSKKN
                     QLEKDVDLIINSHILSNPISQVCHEY"
     gene            2692..3666
                     /locus_tag="EYO86_05525"
     CDS             2692..3666
                     /locus_tag="EYO86_05525"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIC42524.1"
                     /translation="MNINKKYYQAIQENLGNTIQDTRINVPNKKVGKVRDAYFLDNKV
                     LMVSTDRQSAFDRVVAAIPYKGAVLNLVSAWWFTQTKDLFPNHLISVPDPNVSLVEKC
                     SVFPVEFVVRGYITGTTETSLWTVYGDGAREYCGNSLPEGLKKNNKLNEPMLTPTTKD
                     KVHDKPVSKQEIIALGLMSEKDFEVAAKMSLELFSYGQKVAKKNNLILVDTKYEIGKG
                     VDGIIKFVDEIHTPDSSRYWIKKTYQDRIRAGKEPENIDKEFLRLWFAKNCDPYKDES
                     LPSAPDELVVELSARYILLYELITGKKFLFPDLTNINKRISKNIEEIL"
     gene            3663..4058
                     /locus_tag="EYO86_05530"
     CDS             3663..4058
                     /locus_tag="EYO86_05530"
                     /inference="COORDINATES: protein motif:HMM:PF00731.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HIC42525.1"
                     /translation="MKAVLIMGSDSDRGHAEKITSSLEKLGVKFETFVASAHKSPEKV
                     LEIIRTHKKGDKIVFITIAGRSNALSGFVAANTEKPTFACPPFKDMVDMGINIHSTLQ
                     MPSKTPVLTVLDPGNCALAVYRVLNLENL"
     gene            4055..>4834
                     /locus_tag="EYO86_05535"
     CDS             4055..>4834
                     /locus_tag="EYO86_05535"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:PF00206.18"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIC42526.1"
                     /translation="MTNISPLDNRYKKHVSELNEYFSEQALMKFRLYVEVNYLIFLSQ
                     HRALKNIVSLDKKDQQKLEKIYSNFDQKEYNEVKRIETKTKHDVSAVIQYLSKKVEKN
                     VSRALVPWVHFGLTSEDINSAAYSLMIKEAVQSSMLKTLEKLSGALKKLVSQNKGFPM
                     LALTHGQPATTTTLGKEMAVFHARLSRQIKQLKKHELLAKFSGATGTLAAHKVAFPKE
                     TWVSFSEKFIKTLGLKNNPITTQIEPNDSLAELLQNIIRINN"
BASE COUNT         1716 a          825 c          963 g         1330 t
ORIGIN      
        1 aaaacaacga tgtttgtctt ttctctgtta ccgaatatag caaaatgctt aatacgttaa
       61 tggaactaat taacaacaaa gcggttttgt cttcaagggt tgttacgggg ggcggtcttg
      121 ttattgctct ttcaaaaatg agttttatga atgaggtggg tatcttgtca acaatgggcg
      181 aggaatcttt tcccgaaaag ctttttaatg agagcttgag tattgttgtc caaatttaca
      241 acagggatgt tggggcagct caaaaaacac tagaaacaaa caatatccct tttgatatta
      301 taggtataac aacacccgaa aaaacaatca aaataaacga cagggtaaac ttattaataa
      361 aagatgctaa aaatatttgg gaaaactcat tgagaaaaaa actattatca tgaaacaaaa
      421 aatttcatat aaagacgctg gcgtagacat cgaaaaaact tatcgggcaa ttgcttcttc
      481 aaaaaaaacg attcaggcaa cttttacaaa aaacgttctt tcttctattg gcgggtttgg
      541 gtcaatgtat agcttaaaag acattttaaa aaactacgat gaaccaatta tggttcaaag
      601 cattgatggg gtgggaacta aaatgtctgt ggctcaacaa tgtaagaact ttaactttgt
      661 tggggttgac ctggtgaatg cgtgttgtaa tgacgttgca gcaactgggg ctaaaccaat
      721 tactttctta gattaccttg caagcagcat actggaccca agtgcgatat ctgaaattat
      781 taaaagcatt gctggtgagt gtaagcggtt gggggtttcg ttggtcggag gagaaaccgc
      841 tgaaatgcca ggaacatatc ataaaaacga atacgatctt gttggtgttg caaccggcat
      901 tatagacaaa aaaaatatca ttgatggctc taaaattaaa aacgggtcta ctattcttgg
      961 tttgccttca agcgggttac acaccaacgg atattctctt gcaagaaaat taatatttga
     1021 aactctcagg cttgatacaa acgacacgct ccccaatcaa aacataacca ttgaacgcgc
     1081 tttgctcgct ccgcacaaaa attatagtac agttattaat ggtttggtca gcgacagaca
     1141 aaacatagaa gggatagccc atattactgg tggcggcctt atagacaaca taccaaggat
     1201 tttgccaaac ggtttagccg cagaaattga tattaataca tggaaaaaaa ttccaatttt
     1261 tgaattttta accaacaacc ttgacctaaa cactaaggag ctatacagaa cttttaacat
     1321 gggaataggg cttattttaa tagtcgaagc aaaccaaaaa ggttccgtga tgaaaacatt
     1381 agaaaacgat gggtgtatag aggttggcag aattataaca agcccctcaa aagaagttta
     1441 cctgagtgaa taataagccc aaaattgcct ttatacagtt ccccggttct aacacagaaa
     1501 acgaaactat tgcaaccatt gaaagaaaca acatggttgc tgtgccccat ttttggaata
     1561 accctttttc tcaactaaga gcttgtgatg gttatattat tttaggtggt ttttcatacg
     1621 aagaccgttc aaggtctggc attattgcat cgctagaccc cattatggaa gaggttaaaa
     1681 aggcaattgt tttggggaag cctgttttgg gcatttgtaa cggtgctcaa attttagttg
     1741 aaagcggaat tgttcctggc aacgaaagct ttggaactat agctagctta acacaaaaca
     1801 agcgcgttca aaacgggcaa attgtcggta cgggctattt caacacatgg tgtcacatta
     1861 aaccaaacca aaaaagcagg tcagctttta tagataaaaa caatgggggc gtccttagtg
     1921 ttccattggc ccacgcagag gggaggttta tgatgagcaa aaaattaagc agtattataa
     1981 aagaaaacaa ccttgcaaca tatcgctatt gtaccgaaag cggtgagctc acatcagatt
     2041 tccctacaaa ccctaatggg tcggttgata atgttgcagc tttagggaat ttgcccggca
     2101 acgccatggc aattatgcca cacccagaaa gaacaattgg tggagggggt gatgcagttt
     2161 ttaaagcaat gcataaatac atagtacaaa aaggtgccta tagctatagg gctttaaaat
     2221 atgctgccga aaagataacc gtttcaccct ttgaaaaaag cccaacaaca aaagaaatta
     2281 ttatatcaac gattattgct gacaacgaag ccagctctgt agaaaaatgt gtccaaaagc
     2341 tgggggcaga tacaacagta aaaagatatg ttcattttga gatagaggct gacaaaaata
     2401 tggacctgaa ggatgtttat aaaacaaacg tattgtttaa ccctagcaaa gaatttatgg
     2461 ttaatcttga taacacaaac aaaacaaaaa ggtttttagt tagagaaaaa gaaaacatag
     2521 agggaagaag aattaaggaa attttacaga agaggtttgg ttttgataaa attaaaaata
     2581 ttcaaaaaag tgttgtttgg gaaatttcct caaaaaagaa tcaattagaa aaggacgtgg
     2641 atttaattat aaatagtcat attttatcca accctatttc acaagtctgt catgaatatt
     2701 aacaaaaaat attatcaggc gattcaagaa aacctgggta atactattca ggacaccaga
     2761 attaacgtcc caaacaaaaa ggttggcaaa gttcgtgatg catattttct agacaacaaa
     2821 gtattaatgg tttccactga tagacaaagc gcttttgata gggttgtggc ggcaattcca
     2881 tacaaggggg ccgtattgaa ccttgtaagc gcctggtggt ttacgcaaac gaaagatctc
     2941 tttccaaacc atttaatttc tgttcccgat ccaaatgttt cgttagtaga aaaatgtagc
     3001 gttttccctg ttgagtttgt tgttcgcgga tatataacgg gaacaaccga aacgtcttta
     3061 tggacagttt atggtgatgg tgcaagagag tattgtggaa acagcttgcc agaaggctta
     3121 aaaaagaaca acaagcttaa tgagccgatg ttaaccccaa ccacaaagga caaagttcat
     3181 gacaagcctg tttctaaaca agaaattatt gctttagggt taatgtcaga aaaagatttt
     3241 gaggttgcgg caaaaatgag cttggaactt tttagttacg ggcaaaaagt tgcaaaaaaa
     3301 aataatttaa ttttggtaga cacaaaatat gaaattggaa agggggtgga cggaataatt
     3361 aagtttgttg atgaaattca taccccagac tcaagcaggt attggattaa aaaaacatac
     3421 caagaccgta ttcgcgctgg caaagaacca gagaatatag ataaagagtt tttaaggctg
     3481 tggtttgcaa aaaactgtga tccatacaaa gacgagagct tgcctagcgc tccagacgaa
     3541 ttagttgttg agctttcagc aagatatata ttactttatg aactaataac aggaaaaaag
     3601 tttttgtttc ctgatttaac aaatatcaac aaaagaatat caaaaaatat tgaggagata
     3661 ctatgaaagc tgttttaatt atgggctctg attctgatag gggtcacgct gaaaaaatta
     3721 cgtcttcgtt agaaaagctt ggagtaaaat ttgaaacatt tgttgcttct gcacacaaga
     3781 gccctgaaaa ggtcttagaa attattcgga cccacaagaa gggtgacaaa atagttttta
     3841 ttaccatagc cggaagatca aacgctttat ctgggtttgt tgcagccaac acagaaaagc
     3901 caacctttgc ctgccctcca ttcaaagaca tggttgatat gggtataaat attcactcta
     3961 ctctacaaat gccaagcaag acacctgttt taactgttct tgacccggga aattgtgctt
     4021 tagccgttta tagggtttta aacttagaaa atctatgacc aacatctccc ctttagacaa
     4081 cagatataaa aaacacgtta gcgagcttaa tgaatatttt tcagagcaag cgctgatgaa
     4141 gtttcgtcta tatgtggagg ttaactattt aattttttta tcacagcatc gagcattaaa
     4201 aaatattgtt tctcttgata aaaaagacca acaaaaacta gaaaaaattt attcaaactt
     4261 tgaccaaaaa gaatataacg aggttaaaag aattgaaaca aaaacaaagc atgacgttag
     4321 tgcggttata caatatttgt ctaaaaaggt agaaaagaat gtttcgaggg cactggtacc
     4381 atgggtacac tttgggctaa cctccgaaga cataaacagt gccgcttatt ctttaatgat
     4441 taaagaagct gtccagtctt ccatgttaaa aaccttggaa aagctgtctg gggctttaaa
     4501 aaaattagtg tcacaaaata aaggctttcc gatgttagcg ctaacacacg gacagcccgc
     4561 taccacaacc actcttggaa aagaaatggc tgtttttcac gcaaggcttt ctagacagat
     4621 taaacaatta aagaaacatg aattgttagc aaagttttct ggagcaaccg gcacgcttgc
     4681 tgcgcacaag gttgcttttc caaaagagac ttgggtttct ttttctgaaa agtttataaa
     4741 aactctcgga cttaaaaata atcctattac aacacaaatt gaaccaaacg attcgcttgc
     4801 ggagctgcta caaaacatta taagaataaa caat
//
LOCUS       DTVV01000464            2332 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2403670, whole genome shotgun sequence.
ACCESSION   DTVV01000464 DTVV01000000
VERSION     DTVV01000464.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2403670"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="EYO86_05540"
     CDS             complement(<1..191)
                     /locus_tag="EYO86_05540"
                     /inference="COORDINATES: protein motif:HMM:PF01168.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42527.1"
                     /translation="MIKPNAILEIKTKNLIHNYKTLSTIASSSLAGATLKANAYGIGD
                     LEIYKLLYKNNCRHFFFAS"
     gene            complement(188..1699)
                     /locus_tag="EYO86_05545"
     CDS             complement(188..1699)
                     /locus_tag="EYO86_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIC42528.1"
                     /translation="MPLNNLQSVRNKENKEVSEKFQNFDAEIAVIGCLLWDNKSYEKI
                     ADFLIEDHFIDLNNKNIFKTIKRLLDKNILVTPITLKNYLEENDKDSFDNYTYLNQIK
                     DSAPSTQNAYQYARLLYDLHIKRSLIGIGKNIIQDTISNEEDLEGINLIENAENDLYN
                     LSQTGSSDRKYSLFGESLKKAIDVIDQSFKREGKIAGLPSGLKDLDKKLGGFHNSDLI
                     IIAGRPSMGKTALGTNIAFNAAKKFKEKEDEFGNKTTIDGGKIAFFSLEMSSEQLATR
                     VLAEQSKISGDKMRKAELNKEDFKKIAKVSSELENLNLVIDDNPILTVASLRARARRL
                     KRLYDIDMIIIDYLQLMSGSQNVRNDGRVQEISEITRGLKAIAKELNIPIIALSQLSR
                     QVEQREDKRPQLADLRESGTIEQDSDVVMFIFRESYYLERMEPVKKPDEQNDRYNERH
                     QRWRELCESRYNIADIIIAKQRHGPTGAIKTHFDPNFTKFSDLTRTEYDNIIE"
     gene            complement(1787..2230)
                     /gene="rplI"
                     /locus_tag="EYO86_05550"
                     /pseudo
     CDS             complement(1787..2230)
                     /gene="rplI"
                     /locus_tag="EYO86_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
     gene            complement(2231..>2332)
                     /locus_tag="EYO86_05555"
     CDS             complement(2231..>2332)
                     /locus_tag="EYO86_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18"
                     /protein_id="HIC42529.1"
                     /translation="RISNVSNKKQKELSKAIKRARFLGLMSYTLKVF"
BASE COUNT          712 a          369 c          275 g          976 t
ORIGIN      
        1 aatgaagcaa aaaaaaaatg acggcaatta tttttgtaaa gtaatttata tatttctaaa
       61 tcacctatac cataagcgtt tgcttttaat gtagcgcccg ctaggcttga actagcaatc
      121 gtacttaatg ttttataatt atgtattaag ttttttgttt ttatttcaag aatagcattg
      181 ggttttatca ttcaataata ttatcatatt ctgttcttgt taaatcactg aattttgtaa
      241 aatttggatc aaaatgagtt ttgattgctc ctgttggacc atgtctttgt ttagctataa
      301 ttatgtctgc tatattatat ctactttcac ataattcgcg ccacctttga tgcctctcgt
      361 tatatctatc attttgctca tcaggttttt taacaggttc cattctttct aaatagtaac
      421 tctctctaaa tataaacatc acaacatcag aatcttgctc tatagtccca ctttctcgca
      481 gatcagctaa ttgaggcctt ttgtcttctc tttgttctac ttgtcttgaa agttgagata
      541 aggctataat tggaatattt aattcttttg caatagcttt caatcctctg gttatctcag
      601 aaatttcttg aactcttcca tcatttctta cattttgtga acccgacatt aattgaagat
      661 aatcgataat aatcatatca atatcataaa gccttttgag tcttctagct cttgctctca
      721 aagaagctac agttaatata ggattgtcat caataactag atttaaattt tctaattctg
      781 aactaacttt ggcaattttt ttaaaatctt ctttatttaa ttctgctttt ctcattttat
      841 caccacttat ttttgattgt tctgccaaaa ctctagtagc aagttgttct gatgacatct
      901 ctaaagaaaa aaaagctatc ttacctccat caatagtagt tttatttcca aattcatctt
      961 ctttttcttt aaattttttt gcagcattaa aggcaatgtt tgttcccaaa gcagtttttc
     1021 ccattgaagg cctacctgca ataattatta aatcagaatt atgaaagcca ccaagttttt
     1081 tatctaaatc tttcaatcct gatggtaatc cggctatttt accttctctt ttaaatgact
     1141 gatcaataac atctattgct ttttttaaag actcaccaaa aagagaatat ttacgatctg
     1201 aacttcctgt ttgtgacaaa ttgtataaat cattctcagc attttcaatc aaatttatcc
     1261 cttcaagatc ttcttcgtta gatattgtgt cttgaataat attttttcca attccaatta
     1321 aacttctttt tatgtgtaaa tcatatagta accttgcata ttggtaggca ttctgggttg
     1381 atggagcaga atcttttatc tgattaagat atgtgtaatt atcaaaagaa tctttatcat
     1441 tttcttccaa ataatttttt agagtaatag gagtaaccaa aatattttta tctaaaagtc
     1501 gtttaattgt tttaaagata tttttattat tcagatcaat aaaatgatct tcaattagaa
     1561 aatctgcaat tttttcataa gatttattat cccataaaag acagccaata actgctattt
     1621 cagcatcaaa attttgaaat ttttcagaaa cttctttatt ttctttattt cgaacagact
     1681 gcaaattgtt taatggcaca ttttttcttt aaacgaaaaa tgatgtttaa caatatgttg
     1741 aattagaaaa taaagttaat atcttttaat aatgtgtata aaattattaa gtttttttga
     1801 ctgttacttt taattgttca attatccctt cgtagggact aatttctatt agatgctcac
     1861 ccaatgttct tatagtttgt attattttaa tatcatcgga aaataatttt attttttgac
     1921 gattaaagta attaataatt tcattttttg acacagatcc ataaagctgg tctttttcat
     1981 cagcttcttt aacaaattca atattaattt ttttcacagc ttcaattaag tcaatagctt
     2041 tctgtttttt ttcgctttct ttaatattga tttcttcttt aattttttca aaatatttta
     2101 ggttagattc ggtgtctcta agtgccatct tatttggaaa aagaaaattt ctagcaaatc
     2161 cagtctttac gtgaacttga tctccaactt taccaagttt ttttatattg gttgttaata
     2221 ttatcttcat ctaaaatact tttagagtgt atgacattaa acctaaaaaa cgcgctcttt
     2281 ttatagcttt tgacagttct ttttgttttt tatttgatac attactaatt ct
//
LOCUS       DTVV01000465            2430 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2404262, whole genome shotgun sequence.
ACCESSION   DTVV01000465 DTVV01000000
VERSION     DTVV01000465.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2404262"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(153..1178)
                     /locus_tag="EYO86_05560"
     CDS             complement(153..1178)
                     /locus_tag="EYO86_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC42530.1"
                     /translation="MQISPLNQRRFNNFKSNKRGWYSFWIFSFLFLISIFADFIANDK
                     PLLVRYDNAFYLPIINEYPETIFGGDFETEADYRDPFVKKLINSNGWIIMPIIPYSYN
                     TIIRDLNVPAPAPPSQKNWLGTDDQARDVLARLIYGFRISILFGFTLTFFSMIIGVSA
                     GAVQGYFGGKTDLFFQRFMEIWSAIPALYVLIILASIVQPNFWWLLIILLIFSWMGYV
                     GVVRAEFLRARNFDYVRAAKALGVSNSTIMFRHLLPNATVATITFLPFSLSASVTALS
                     GLDFLGFGLPPGSASLGEMVNQGRNNLQAPWLGLTSFFTLGLMLGLLVFVGEAIRDAL
                     DPRKTFI"
     gene            complement(1178..2284)
                     /gene="yejB"
                     /locus_tag="EYO86_05565"
     CDS             complement(1178..2284)
                     /gene="yejB"
                     /locus_tag="EYO86_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HIC42531.1"
                     /translation="MTAYIIRRLLLVIPTLLGIMLINFAVIQIVPGGPVEQMIAQMTG
                     TAVESTARFSGGGEGENLNSISISDSSNFDSKYRGAQGLDPDIIKEIEEMYGMNRPAH
                     IRFFSMLKDYLTFDFGESFFRDQKVISLVLDKMPVSISLGLWTTLLVYLVSIPLGIRK
                     AIRDGSQFDITSSTIITIGYAIPNFLFAVILIVFFAGGRYYNIFPLRGLVSENFDELN
                     ILLKIKDYFWHLTLPLLAMLVSGFAGLTFLTKNSFLDQINQQYVMTARAKGLSENKVL
                     YGHVFRNAMLIVIAGFPSSFIGILFSSSLFIEVIFSLDGLGLLGYEAALTRDYPVIFA
                     TLYFFSLLGLIMGILGDIMYSIIDPRIDFESRDN"
BASE COUNT          863 a          452 c          405 g          710 t
ORIGIN      
        1 atttttttcc tatttgggaa ttgatactaa tgcacttctt ggtaccccct taataattat
       61 atttaccgtt gtcgtggtgt ttatatttct gggacaggtt ttattgcggt ccggtggttc
      121 tgaatttttt acagatatta aattttccat aattagatga atgttttcct tggatcaagg
      181 gcgtctctta tggcttctcc tacgaacacc agcaaaccta acattaagcc tagtgtaaaa
      241 aaacttgtta atcccaacca gggagcttgt aagttatttc taccttgatt taccatttct
      301 cccaatgaag cggaaccagg aggcaaacca aaacctaaaa aatccaaacc agacaaagca
      361 gtaacagaag cgcttaaact aaaaggaaga aaagtaattg tggctacagt ggcgttggga
      421 agtaagtgcc tgaacataat agttgagttt gaaacaccca atgcttttgc agccctcaca
      481 taatcaaagt tgcgagcacg taaaaattca gctctaacca cacctacata tcccatccaa
      541 ctaaatatta ataaaataat gagcaaccac cagaaattgg gctgaactat actagccaag
      601 atgattaaaa catagagggc tggaatagct gaccaaatct ccatgaatcg ttgaaaaaat
      661 aaatctgttt ttccaccaaa gtaaccttga acggcacctg cagaaacacc tatgatcatt
      721 gaaaaaaatg ttagagtaaa tccaaacaat atagagattc ggaaaccgta aattaatctt
      781 gcaagcacat ctcttgcttg atcatcggtt cctagccaat ttttttgaga tggtggtgct
      841 ggagcaggaa catttaggtc tcttatgata gtattataag agtagggtat tataggcatt
      901 attatccatc cattgctgtt tattaacttt tttacaaaag gatcgcggta atccgcttct
      961 gtttcaaaat caccgccaaa aatagtttca ggatattcat ttataatagg taaataaaag
     1021 gcattatcat agcgcactaa caaaggttta tcattagcaa tgaaatctgc gaaaattgaa
     1081 atcaaaaaca aaaacgagaa aatccaaaaa gagtaccaac cgcgtttatt ggatttgaaa
     1141 ttattaaaac gcctttgatt gagaggtgat atttgcatca attatctctc gattcaaaat
     1201 caatccttgg atcaataatt gaatacataa tgtctcctaa aatgcccatg atgaggccca
     1261 ataaggaaaa gaaatacaat gtcgcaaaaa taactggata atcccgagtt aaagcggctt
     1321 cgtaaccaag taatcccaat ccatctagtg aaaagattac ttcaataaat agggagcttg
     1381 aaaaaagtat acctataaag gaagatggga aacctgcaat cacgattaac atggcattgc
     1441 gaaacacatg accatataaa actttatttt ccgacaaacc ttttgccctc gctgtcataa
     1501 cgtattgctg attgatttgg tcaaggaaag aatttttagt taaaaaggtt aatccagcaa
     1561 aaccgctgac caacatagct agcaaaggaa gtgtgagatg ccagaaataa tcttttattt
     1621 ttaataatat atttaattca tcgaaatttt cggataccaa accccgcaat ggaaaaatgt
     1681 tatagtacct acccccagca aaaaaaacaa ttaaaatgac cgcaaataaa aaattgggaa
     1741 tagcgtatcc aatagtaatg atggtgctag atgtaatatc aaattgacta ccatcacgaa
     1801 tggctttgcg tattccaagt ggaatagaaa ctaaataaac aagtagtgtt gtccacaatc
     1861 ctaaagaaat agagacaggc attttatcta aaactaagct aatcactttt tgatcacgga
     1921 aaaaactctc tccaaaatca aaagttaaat aatcttttaa catactaaaa aatcgaatat
     1981 gtgccggtct attcatcccg tacatttctt caatttcttt aattatatct ggatctaaac
     2041 cttgggcacc cctgtattta gaatcaaaat ttgaagaatc agaaatacta atactattta
     2101 aattttctcc ttcaccacct cctgaaaatc ttgctgtaga ttcaacagct gttccggtca
     2161 tctgcgcaat catttgttcc accggtcccc cgggaacaat ttgaattaca gcgaaattga
     2221 ttaacataat tcctagcaaa gtgggtatta ctaggagcaa ccgtctgata atatatgcgg
     2281 tcatagaaag ataattatat cattaattat gaacaaaaga tgttaattat aagagctatt
     2341 gaagggattt ttttctctgg ttaattgtac tggccttgtc agtatcaacc caccatgtgt
     2401 tagttccaag ggaatatttt ggtctcactg
//
LOCUS       DTVV01000466            3021 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2409451, whole genome shotgun sequence.
ACCESSION   DTVV01000466 DTVV01000000
VERSION     DTVV01000466.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2409451"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="EYO86_05570"
     CDS             complement(<1..389)
                     /locus_tag="EYO86_05570"
                     /inference="COORDINATES: protein motif:HMM:PF13476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42532.1"
                     /translation="MGYFRSIKLKNYRNFLDSSFEFDDKCNIIIGKNGSGKTNVLESL
                     SLFEKGRGFRKEKIINLVNYETNEKNFNIRSIFHHEDTDYTVDVSNIDKNEKNLKKIF
                     INNSSDVDSLKYFENLLSFIYFLPGME"
     gene            complement(396..1511)
                     /locus_tag="EYO86_05575"
     CDS             complement(396..1511)
                     /locus_tag="EYO86_05575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIC42533.1"
                     /translation="MKFTVEKNTLLKSLSHVQSIVEKKNTLPILSNILLDAKENSLIL
                     SATDMDISITEKLNCNIIEEGSITVSAHTLYDIIRKLPDTSEIEIISNDGKIISLRSG
                     KSKFSLGCLPKKDFPIIEISDLEIEINIDSHKFLRLMDKTRFAISNEETRYFLNGIYF
                     HKKKEDHSNLLSVVATDGHRLAKIDFNFNEDSEEISGVIVPKKTVNELCKLLAEYDGS
                     IKINLDSNKIVFFINESILISKLIDGNFPDYDRVIPNDNNHILKINRHDFSMAVDRVS
                     TITTDKSPIIKFKLLNNVMNLSSVNSDSGTATEDITTIYSGNEIEIGFNSKYILEMIN
                     NLEDDEITLSFKDSSSPVMATEESNPNLIYVLMPMRV"
     gene            complement(1537..2931)
                     /gene="dnaA"
                     /locus_tag="EYO86_05580"
     CDS             complement(1537..2931)
                     /gene="dnaA"
                     /locus_tag="EYO86_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIC42534.1"
                     /translation="MKDSFLDFNEKNWPLLKRNIKKIVGDTAYNNWFKQLSFISIENT
                     TITLSVPTKFLRDWIVNNYADKVKNECKKYVENIEIIKFVVKPNGGRVVPGTARTIKD
                     TDNHWKTTMDIRSNQTSSYPSDFGAPLDPRFNFDNFVVGKPNELAFAASQRVAESQTV
                     TFNPLFLYGGVGLGKTHLMHAIGWKIKEQQPERKVIYLSAEKFMYQFVRALRYKDTSA
                     FKEQFRSIDVLMIDDVQFISGKDNTQEEFFHTFNALIEQNRQIVISADKSPSDLDGVQ
                     ERLKSRLGCGLVADIHPTTYELRLGILIEKAQKRGVEIPPKVLEFLSHRIVSNVREME
                     GALNRLVAHATLVGTAISVETAQIVLQDLLKSNNKKITIEEIQKKVAEHFNIRLTDMH
                     SPRRSRSVARPRQIAMYLAKSITSRSLPEIGRKFGGRDHTTVMHAVKKVEELKHEDNN
                     FNEDIELLKRLIDS"
BASE COUNT         1011 a          490 c          372 g         1148 t
ORIGIN      
        1 ctctccattc caggcaaaaa ataaataaag gataataaat tttcaaaata ctttaaagaa
       61 tctacatcag aactattatt aatgaatatt ttttttaaat ttttttcatt tttgtcaata
      121 ttggaaacat caacagtata atcagtatct tcatgatgga aaattgaacg tatattaaaa
      181 tttttttcat ttgtttcata gttgactaga ttaataattt tttctttcct aaatcctctt
      241 cctttttcaa ataaagacaa cgattctaaa acatttgttt tccctgaacc atttttccca
      301 ataataatat tgcatttatc atcaaattcg aaagatgaat ctaaaaaatt tctatagttc
      361 tttaatttaa tgcttctaaa ataacccaag gaaaactata ctcgcatagg catcaaaaca
      421 tatattaaat taggattgga ttcttcagta gccataacgg gagaagatga atccttaaag
      481 cttaatgtaa tttcatcgtc ttcaagatta ttaatcattt ctaatatata ctttgagtta
      541 aaaccaatct ctatttcgtt accagaatat atggttgtaa tatcttcagt tgctgtacca
      601 ctatcagaat tcactgaaga caaattcatt acattattaa gaagtttaaa tttaataata
      661 ggagatttat cagttgtgat tgtacttacg cgatcaacgg ccatgctaaa atcatgccta
      721 ttaatcttta aaatatgatt attatcattt ggtataacac ggtcgtaatc tggaaagttt
      781 ccatctatta attttgatat taagattgat tcattaataa aaaaaactat tttattagaa
      841 tccaaattaa tttttattga accgtcgtat tcagcaagta atttacacag ctcattaact
      901 gtttttttag gtacaataac tcctgaaatt tcttcagagt cttcattaaa attaaaatct
      961 atttttgcca atctatgtcc atcagttgct acaacagata ataaatttga atgatcttct
     1021 ttttttttat gaaaataaat tccattaaga aaatatcttg tctcttcatt cgatattgca
     1081 aaacgagttt tatccattaa cctcagaaat ttatgagaat ctatattaat ttcaatttct
     1141 aaatcactaa tctctataat tggaaaatct tttttaggta ggcatcctaa agagaatttt
     1201 gactttccag atctaagact tattattttc ccatcattag atattatttc aatttcactt
     1261 gtatctggta gctttctaat aatatcataa agcgtatgtg ctgaaactgt gattgagccc
     1321 tcttcaataa tattacaatt taatttttca gtaattgaaa tatccatatc tgtagcagac
     1381 aaaattaaag aattttcttt tgcatcaagt aaaatattgg ataatatcgg aagggtgttt
     1441 tttttctcaa ctatactttg aacatgtgaa agtgatttga gaagagtgtt tttttcaaca
     1501 gtgaatttca ttaaataaat ttataattaa aaaatcttaa gaatctatta atctttttaa
     1561 taactcaata tcttcgttaa aattgttatc ttcatgtttt aattcctcga cttttttcac
     1621 tgcatgcatt actgttgtat gatctcttcc tccaaattta cgccctattt caggcagtga
     1681 acgggaagtt atcgattttg ctaggtacat tgctatttga cgaggtcttg ctacagacct
     1741 ggatctacgt ggagaatgca tatcggttaa tctgatatta aaatgttcag caactttttt
     1801 ctgaatttct tctattgtaa tttttttgtt atttgatttt aataaatcct gtaatacaat
     1861 ctgcgctgtt tcaactgaaa tagctgtgcc aactaatgtt gcatgggcta ccaatctatt
     1921 tagagcgcct tccatttctc taacattaga aacaatacga tgagataaaa attctaaaac
     1981 tttaggaggt atttcaacac ccctcttttg tgctttctct attaatatac ccaaccttaa
     2041 ttcatacgtc gttggatgaa tatccgctac taaaccacat cccaatctag attttaatct
     2101 ttcttgaact ccatcaagat ctgaaggtga tttatcagca gaaataacaa tttgcctatt
     2161 ttgttctatc aaagcattaa acgtatgaaa aaattcttct tgtgtattat cttttccact
     2221 aataaattgt acatcatcaa tcattagaac atcaatggat ctaaattgtt ctttaaacgc
     2281 agatgtatct ttatatctta acgctctaac aaattgatac ataaattttt ctgcagaaag
     2341 ataaattact tttctttctg gttgttgttc ttttatcttc cacccaatag catgcattaa
     2401 atgagtttta cctaaaccta caccgccata taaaaataat ggattgaaag taactgtttg
     2461 agattctgct actctttgag aggcggcaaa agctaattca ttaggctttc caaccacaaa
     2521 attatcaaag ttaaaacgag gatctaatgg agctccaaaa tcacttggat atgaagaagt
     2581 ttgatttgat cgaatatcca ttgttgtttt ccaatggtta tcagtatctt ttatggttct
     2641 agccgttcct gggactactc ttcctccatt aggtttaaca acaaatttta taatttcaat
     2701 attttcaaca tattttttac attcattttt tactttgtca gcataattat tgactatcca
     2761 atctcttaaa aatttagttg gaacagataa ggttattgtt gtgttttcaa tagaaataaa
     2821 agagagttgt ttgaaccaat tattgtaagc agtatctcca actatttttt taatattcct
     2881 ttttaaaaga ggccaatttt tctcattaaa atccaaaaag gaatccttca ttcattcacc
     2941 tctaccagtc tttatttgtt tgcagtgcaa agactaaaag tcacatacta aataatcacc
     3001 aaattattat gatagacaac a
//
LOCUS       DTVV01000467            1715 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2409763, whole genome shotgun sequence.
ACCESSION   DTVV01000467 DTVV01000000
VERSION     DTVV01000467.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2409763"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="EYO86_05585"
     CDS             <1..351
                     /locus_tag="EYO86_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIC42535.1"
                     /translation="DNQFSGLIDFYFSCYDFLAYDIAITVNAWCFNDKGKFDNDKFQS
                     LIVGYQVNRLLSHDEKQSMPVILRGAAIRILITRLYDQLYHPAGAFVIPKDPLEYFSI
                     LQFHQTHNFFEVNI"
     gene            348..779
                     /gene="rnhA"
                     /locus_tag="EYO86_05590"
     CDS             348..779
                     /gene="rnhA"
                     /locus_tag="EYO86_05590"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIC42536.1"
                     /translation="MKITVYTDGACSGNPGIGGWGAVILIEDTKPVHLHGGSDHTTNN
                     QMELTAAIEALKYFSESSTLNFFTDSKYVKEGIESWVHNWKRNGWKTTAKKPVKNKEL
                     WKELDAQITKHTINWQWVKGHAGNVHNETADYLARKFIEDR"
     gene            779..1351
                     /locus_tag="EYO86_05595"
     CDS             779..1351
                     /locus_tag="EYO86_05595"
                     /inference="COORDINATES: protein motif:HMM:PF04264.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42537.1"
                     /translation="MHSNIIIGILILLYCIFPHSIFAKEEWVLDKELSIISFEIPVFL
                     AKNVRGQFTQIEGLIELDPEAKENNKAIFSVDINSIEMNYKKYRNLMLSNVFFNVKQF
                     PIALIDTKKFSYEDESKLEILVELSIKGVTETVPLQLEIKRLAEELVQIKGKLEFSRT
                     AFKIGIGKWSSTAILKDNATIETDLFLFRE"
     gene            complement(1355..>1715)
                     /locus_tag="EYO86_05600"
     CDS             complement(1355..>1715)
                     /locus_tag="EYO86_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HIC42538.1"
                     /translation="LKKKGFNYMDSPGYDPVSVTGQVASGSNVICFTTGRGSCFGFKP
                     SPSIKIATNTNMFNKLEEDMDINAGTIMDGESDIAEIGEKIFHRIISTASGKLTKSEI
                     NGYGDDEFNPWVIGATM"
BASE COUNT          602 a          294 c          283 g          536 t
ORIGIN      
        1 gacaatcagt tttccggatt aatagatttt tatttttctt gttatgattt tcttgcctat
       61 gacattgcca tcactgtcaa cgcttggtgt ttcaatgata aaggaaaatt tgataatgat
      121 aaatttcaat cattgattgt tggttatcaa gtgaacagat tacttagtca tgatgaaaaa
      181 caatcaatgc ctgtcatact acgaggagct gcaatacgta ttttaattac gcgattatat
      241 gaccaactat atcatccagc aggtgctttt gtaattccta aagatccttt agaatatttt
      301 tctattttac aatttcacca aactcataat ttttttgagg ttaatatatg aaaattacgg
      361 tttacacgga cggcgcatgc tcgggtaatc ctggtatagg aggctggggt gcagtcatac
      421 taatagaaga tacaaagcca gttcacctgc acggtggttc agatcataca accaataatc
      481 aaatggaatt aaccgctgca attgaagcgt taaaatattt ttcagaatca tccactctta
      541 atttttttac agacagcaag tacgttaaag aaggaattga gtcatgggta cataactgga
      601 aaagaaatgg atggaaaacg actgcaaaaa aacctgtaaa aaataaagaa ttatggaagg
      661 aacttgatgc acaaataacc aaacacacca tcaattggca atgggtaaaa ggacatgctg
      721 gaaatgttca caatgaaaca gctgattatt tagcaagaaa atttattgaa gacaggtaat
      781 gcactctaat attatcatag gtattttaat attattatac tgcatttttc ctcattcaat
      841 attcgctaaa gaggaatggg tactcgataa agaactttcc atcattagtt ttgagatacc
      901 tgtattctta gccaaaaacg ttcgtggaca attcactcaa atagaagggc tcattgaatt
      961 agaccctgaa gcaaaagaaa ataataaagc aattttctca gttgatatca acagtattga
     1021 aatgaattat aaaaaataca ggaatttaat gctcagcaat gtatttttta atgtaaaaca
     1081 atttcccatt gccttaattg acaccaaaaa attttcctat gaagatgaaa gtaaattaga
     1141 gatcttggtt gaactttcta taaaaggagt taccgaaact gttccccttc aacttgagat
     1201 caaacgttta gcggaagaac tagtgcagat taaaggaaaa ttagaattct cacgaacagc
     1261 ttttaaaatt ggaataggaa aatggtcatc aacagccatt cttaaagata acgccaccat
     1321 tgaaacagac ttatttttat ttagagaata atacttacat cgttgcacca atgacccacg
     1381 gattaaattc atcatccccg taaccattga tttcactttt tgttaatttt ccagaagccg
     1441 tactgataat tcgatgaaat attttttcac ctatttcggc aatatctgat tcaccatcca
     1501 taatcgtacc agcattaata tccatgtctt cttctaactt gttaaacata tttgtattcg
     1561 tagcaatttt aatgcttggt gagggcttaa agccaaaaca tgaaccgcgg ccagtggtaa
     1621 aacagataac gttgctacct gaagcaactt ggcctgttac tgatactgga tcataaccag
     1681 gagaatccat ataattaaat ccctttttct ttaga
//
LOCUS       DTVV01000468            1639 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2412702, whole genome shotgun sequence.
ACCESSION   DTVV01000468 DTVV01000000
VERSION     DTVV01000468.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2412702"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="EYO86_05605"
     CDS             complement(<1..820)
                     /locus_tag="EYO86_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019294996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC42539.1"
                     /translation="MTRFNAWNVFKNGLIGQTDWDRQWRDPEPKKEYDVIIIGGGLHG
                     LATAYYLANNHNIHNIAVVEKGWIGGGNAGRNTTIIRSNYMMPGNHQFYEHSLKLWEN
                     LSHELNYNVMFSQRSHISLFHSPAARDAAARRYNIMSLNGTEAELWNLDQLKKNIPHL
                     NYEDARFPIMGAAVQRRAGNARHDSVAWGYARAADQLGVDILQNCEVTGVKRNKGRIE
                     SLQTSQGEIKAKKIGFAVAGNTSRLWEMAELGNLPIETHKLQAFVTESIKPLLDQ"
     gene            complement(912..>1639)
                     /locus_tag="EYO86_05610"
     CDS             complement(912..>1639)
                     /locus_tag="EYO86_05610"
                     /inference="COORDINATES: protein motif:HMM:PF05544.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42540.1"
                     /translation="EKNLIKMTEPLSEFFLEAPGGLIKIKAHCENKKVKSVEIQNLPS
                     FVDQLDVKLNTPNFGPLIVSTVFGGDSFLICDAGDFDLSIQPENAKKFVEIAQEIIPL
                     ANKQLGFHHPSLTSLNYISFCQFIEPVVVNAEGNKEGKNTVCIRPGKLDRSPCGTGTS
                     ARLALMREKNEINLNETFISKSIIGSLFESTIVDEFMSNNKNMVTPTITGSAFITGKQ
                     ELYLSKNDPFPKGYRLNDTWPQY"
BASE COUNT          476 a          332 c          263 g          568 t
ORIGIN      
        1 cctggtctag aagtggcttg atcgactccg ttacaaatgc ctgtaacttg tgcgtctcaa
       61 tgggaaggtt tcccaattcg gccatttccc aaagtcgcga agtgtttccg gccacggcaa
      121 aaccaatttt ttttgcttta atttctcctt gtgatgtttg taaactttca atacgtccct
      181 tatttctttt tactcctgtt acttcacagt tttgaagtat atcaactccc aattgatcag
      241 cggcccttgc gtatccccag gctaccgaat catgccttgc gttacccgcc cgtctttgta
      301 ccgctgctcc cataattgga aaacgagcgt cttcataatt taaatgaggt atattttttt
      361 ttaattgatc aagattccat aattcagctt ccgttccatt taaactcata atgttgtaac
      421 gtctcgctgc tgcatctctt gctgctggac tatgaaacaa agagatgtgg gacctctgac
      481 tgaacataac attgtaattt aattcatgac tcaaattttc ccataacttg agtgaatgct
      541 cataaaattg atggttgcct ggcatcatgt aattagaacg aatgatagtt gtattacgcc
      601 ctgcattacc tccgcctatc cacccttttt ctacaacggc aatattgtgt atattatgat
      661 tattggctaa ataataagct gttgccaaac catgtaatcc tcccccaatt ataattacat
      721 catattcctt tttgggttca ggatctcgcc attgcctgtc ccagtcagtt tggcctatga
      781 ggccattttt aaatacattc catgcattaa aacgtgtcat aagttcattc tatattgttt
      841 tataaagtcc aaaatataat gtttctaaag ttgaaataat tgaaaaaata tccttaattt
      901 cagtcataat attaatactg aggccatgta tcattaagtc ggtaaccctt aggaaatgga
      961 tcgtttttgg atagatacaa ctcttgctta ccagttataa aagctgaacc agttatagtg
     1021 ggcgttacca tatttttatt attgctcata aactcatcta ctattgtaga ttcaaacaaa
     1081 gatccaataa tagattttga tataaatgtt tcattgagat tgatttcatt tttttctctc
     1141 attagagcca aacgagcgga cgtgcctgta ccacatggtg atcgatcaag tttgcctggt
     1201 ctaatgcata ccgtattttt tccttcctta ttgccctctg catttactac aacaggttcg
     1261 ataaactgac aaaaggaaat ataatttaag gatgttaatg atggatgatg aaaacccaat
     1321 tgtttatttg caagtgggat tatttcttgt gcaatttcaa caaatttctt tgcattttct
     1381 ggttgaattg ataaatcaaa atcaccggca tcacaaatta gaaaactatc acctccaaaa
     1441 accgtactaa caataagagg accaaaattt ggggtattta atttaacgtc cagttggtct
     1501 acaaaagaag gaagattttg aatttctact gacttaactt ttttgttttc acagtgagcc
     1561 ttaatcttaa ttaaccctcc cggggcttct aaaaaaaact cagacaacgg ctctgtcatt
     1621 tttattaaat tcttctcga
//
LOCUS       DTVV01000469            1134 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2415540, whole genome shotgun sequence.
ACCESSION   DTVV01000469 DTVV01000000
VERSION     DTVV01000469.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2415540"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="EYO86_05615"
     CDS             <1..116
                     /locus_tag="EYO86_05615"
                     /inference="COORDINATES: protein motif:HMM:PF00828.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal protein L15 family protein"
                     /protein_id="HIC42541.1"
                     /translation="DVGELSYPIIIEVSYASKKAIKKVEKIGGKVNLIKVL"
     gene            124..>1134
                     /gene="secY"
                     /locus_tag="EYO86_05620"
     CDS             124..>1134
                     /gene="secY"
                     /locus_tag="EYO86_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIC42542.1"
                     /translation="MASVAEKLANNLNFSALGKATELRNRLLFTLGALVIFRLGTFLP
                     IPGINPIALQQFFEQQSSGILGIFDTFSGGALGRMGIFALGVMPYISSSIIMTLMQSS
                     IPHLKALKEQGTKGRRKIIQYSRYITVLISALQGYGVSIGLESMQTAYGNIVIEPGIF
                     FKITTVITLVGGTIFLMWLGEQITSRGVGNGISLIITAGIVANLPAALVSTLQLGRTG
                     ELSAAFILSILILMLGLIIFIVFIEKAQRRLMVQYPKRQVGNKVYGGQSSHLPLKINT
                     AGVIPVIFASAILLLPNTMSGFGAGSSSEIFLIITSYLGRGQPLYLALFASMIMFFAF
                     FYT"
BASE COUNT          354 a          159 c          207 g          414 t
ORIGIN      
        1 tagatgtagg ggaattatct taccctatta taattgaagt ttcgtatgcc tcaaaaaaag
       61 caattaaaaa agttgaaaaa attggtggaa aagtaaactt gataaaggtt ttataatttt
      121 attatggctt ctgttgctga aaaattagct aataatttaa actttagtgc gcttggcaaa
      181 gctacagagt tgcgtaatag acttttgttt acgttagggg cacttgttat ttttcgttta
      241 ggaacttttt taccaattcc aggtatcaat ccgattgctt tacaacaatt ttttgagcag
      301 caatcttctg gtattttggg aatttttgat actttttctg gaggtgcatt gggaagaatg
      361 ggtatctttg ctttaggtgt tatgccctat atctcatcgt ctattataat gacattaatg
      421 caatcaagta taccacatct taaagctctc aaagaacagg gtactaaagg tagaagaaaa
      481 ataattcaat attctcgtta tattactgtc cttatctctg cattacaggg ttatggcgta
      541 tctataggtt tagagtccat gcaaacagct tatgggaata tagtaattga gccaggtata
      601 ttttttaaaa ttacaactgt tataacatta gttggtggaa caattttctt aatgtggctt
      661 ggagaacaaa ttacctcaag gggagtaggt aatggaattt ctttaattat aaccgcaggt
      721 atagttgcaa atttacctgc tgctttggtt agcactttac aacttggaag aaccggagaa
      781 ttaagtgcag cattcattct ttcaattttg attttaatgt taggattaat aatatttata
      841 gtttttattg aaaaagctca aagaagatta atggttcagt atccaaaaag acaagttgga
      901 aataaagtat atgggggaca aagttctcat ttaccattaa aaatcaatac agctggagtc
      961 atcccagtta tttttgcatc tgctatttta cttttaccaa atacaatgtc tggttttgga
     1021 gcaggatctt caagcgaaat atttttaatc attacaagtt atcttggtag agggcagcct
     1081 ctttaccttg ctttatttgc ttccatgata atgttttttg cattttttta taca
//
LOCUS       DTVV01000470            1212 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2416776, whole genome shotgun sequence.
ACCESSION   DTVV01000470 DTVV01000000
VERSION     DTVV01000470.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2416776"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="EYO86_05625"
     CDS             complement(<1..492)
                     /locus_tag="EYO86_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIC42543.1"
                     /translation="MEHLYLTVIFLFTLFFLLGSGVWIGLALMGVAYVGMEIFTVRPA
                     GDAMITTIWTTSSSWSLTALPLFIWMGEILYRTKLSEDLFKGLAPWMNNLPGRLLHTN
                     IVGCTFFAAVSGSSAATLTTVGKMSIPELRKRNYPENLTIGTLAGSATLGLMIPPSLT
                     LIVY"
     gene            complement(500..1075)
                     /locus_tag="EYO86_05630"
     CDS             complement(500..1075)
                     /locus_tag="EYO86_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009506327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIC42544.1"
                     /translation="MDQKTHPLRNFLDKIYIGAAYLASFFLLCILVVIVVQMVARWTG
                     FVVMGAAAYAGYSMAASTFLAMAYTLHQDGHIRVRIILNKLGKNKKWGERWCYGIGSL
                     LALYFFYYACRGTYFSYILNDISQQDDALPMWIPQMVMVIGTFILATAFIDRLYQTIF
                     FNYDELEIKWKVKDEKAIDQVNDSRDKYYGE"
BASE COUNT          409 a          227 c          178 g          398 t
ORIGIN      
        1 gtaaactatt aatgttaaag atggcggtat cataaggccc aatgtggctg aaccagctaa
       61 agttcctatt gttagatttt ctggataatt tctttttcgt aattctggta ttgacatttt
      121 accaactgtt gtaagggttg ctgctgaaga acctgaaact gcagcaaaaa aagtgcatcc
      181 aacaatatta gtatgcaata accttcctgg taaattgttc atccaaggag ctaatccttt
      241 aaacaagtct tctgaaagtt ttgttctata taaaatttct cccatccaaa taaacaatgg
      301 taatgctgtt agagaccaac tggatgaagt ggtccatata gttgtaatca ttgcatcgcc
      361 agctggacga actgtaaata tttccattcc aacataggca acacccataa gtgctaaccc
      421 tatccaaaca ccacttccta aaaggaaaaa taatgtaaag agaaaaataa ctgttagata
      481 taaatgttcc atctattgat tattctccat aatatttatc tcttgaatcg ttgacttgat
      541 cgattgcttt ttcatctttt actttccatt ttatttccaa ttcatcataa ttaaaaaaaa
      601 tagtttgata taatctgtca ataaaagctg tggctaaaat aaaagttcct attaccatta
      661 ccatttgtgg tatccacatt ggcaatgcat catcttgttg tgaaatatca tttaaaatat
      721 atgaaaaata agttcctctg caagcataat aaaaaaaata aagtgctaat agtgaaccga
      781 tcccatagca ccatctttct ccccattttt tattttttcc taatttgttt aaaattattc
      841 taactcttat gtggccatct tgatgaaggg tgtatgccat agctaaaaag gtcgaggctg
      901 ccattgagta tccggcataa gctgctgctc ccattactac aaaaccagtc catctagcaa
      961 ccatttgcac cactataaca actaatatgc ataatagaaa aaatgaagct aaataggcgg
     1021 ccccaatata aatcttgtcc aaaaaatttc ttaaaggatg tgttttttga tccataaaat
     1081 tatttaaaaa aaattgagcc caaaattaat tgggctcaac tataattgtt attaggatat
     1141 tattggtctg ccttaaatgc gtcgataatt tttgtaccta caggtccagc atttgccatc
     1201 cactcatctg ac
//
LOCUS       DTVV01000471            1575 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2425722, whole genome shotgun sequence.
ACCESSION   DTVV01000471 DTVV01000000
VERSION     DTVV01000471.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2425722"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="EYO86_05635"
     CDS             complement(<1..281)
                     /locus_tag="EYO86_05635"
                     /inference="COORDINATES: protein motif:HMM:PF01725.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HIC42545.1"
                     /translation="MKEIIFFSNNKNKCLEISKLFKDIPIKILSLNNFDKIKSPNESG
                     LTFKENAKIKSSFGLQTFKKTCFADDSGICVEAMNNNPGIKSKKFLETN"
     gene            complement(291..1004)
                     /locus_tag="EYO86_05640"
     CDS             complement(291..1004)
                     /locus_tag="EYO86_05640"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIC42546.1"
                     /translation="MRQDRTFQELRDISFETNVNLHAEGSCLVKFGNTHVLCTASIDE
                     KIPPWIKNSGKGWITAEYGMLPRSTNSRIDREATKGKQSGRTQEIQRLIGRSLRSIVD
                     LESLGQRQIKVDCDVIQADGGTRTASISGGFVALHQAVTHLKKYYNINKSIIKEFLGA
                     VSVGIINDQVNLDLNYQEDTDAQVDANFVISESGKISEIQVTGEEYFFSSDQFNKLFE
                     LSKKGVAEIINKQKNVLFL"
     gene            1067..>1575
                     /gene="hrcA"
                     /locus_tag="EYO86_05645"
     CDS             1067..>1575
                     /gene="hrcA"
                     /locus_tag="EYO86_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037882.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIC42547.1"
                     /translation="MSENLYVELSDRSKQIFKSVVETYLKTGSPSGSETIQKKDGVNL
                     SSSSIRLILANLQKEGLLFAPHTSAGRLPTDKGMRFFVDGLLEFGRLTQDEKNNIKQQ
                     CLSKGTSFQEVLDESSKVISGLSNHTGIVIAPKYQYSIKHIEFIRLNSSQVMSIIASA
                     NGQIENRII"
BASE COUNT          519 a          265 c          221 g          570 t
ORIGIN      
        1 ctgttagttt ctaagaattt ttttgattta atgccaggat tgttattcat ggcctctaca
       61 caaatacccg agtcatcagc aaaacaggtt tttttaaatg tttgcagccc aaaactagat
      121 ttaatttttg cgttttcttt aaatgtaagc ccactttcat tgggtgattt tattttatca
      181 aaattattta aacttagtat tttaattgga atatctttaa acaatttaga tatttctaaa
      241 catttatttt tattgttaga aaaaaaaatt atttctttca cttactattt ttacaaaaaa
      301 agaacatttt tttgtttatt tataatttca gccacacctt tttttgatag ttcaaataat
      361 ttattaaatt gatcactgct aaaaaaatat tcttctccag taacttgaat ttcagaaatt
      421 ttaccacttt cacttatgac aaaatttgca tctacttggg catcagtgtc ttcttgataa
      481 tttaaatcta agtttacttg atcattgatg atacccactg aaactgcacc taaaaactct
      541 ttaataatag acttgttaat attataatat tttttcaagt gtgttactgc ttgatgtaaa
      601 gctacaaaac ccccgcttat agaagcagta cgcgttcctc catctgcttg aattacatca
      661 cagtcaactt taatttgacg ctgaccaagg ctttctaggt ctacaataga tcttaaactt
      721 ctaccaatca aacgttgaat ttcttgggtg cgcccacttt gttttccttt ggttgcttca
      781 cgatcaatgc gtgaattagt ggatcgaggc aacattccat attcagcggt tatccaacct
      841 ttacctgaat ttttaatcca aggaggtatt ttttcatcta ttgaagcagt gcataaaacg
      901 tgagtattgc caaatttaac tagacaagaa ccttcagcat gtaaattaac attagtttca
      961 aaagaaatgt ctctcaattc ttgaaaagtt ctatcttgtc tcataaatta agtacctttt
     1021 tattttttta acttttatta tacttttttt attataaagc cttcatatgt cagaaaactt
     1081 atatgtagaa ttaagtgata gatcaaaaca aatatttaaa agtgttgtag aaacttatct
     1141 aaaaactggt tctccatcag gatcagaaac aatacaaaaa aaagatggag taaatttatc
     1201 ctcttcttcc attcgtttga tattagcaaa tttgcaaaag gaaggcctgc tttttgctcc
     1261 gcatacttct gcgggccgtc tacccacaga caagggtatg agattttttg ttgatggact
     1321 tttagagttt ggaaggctca cacaagacga aaaaaataac atcaaacaac aatgtctttc
     1381 aaaaggaact tcttttcagg aagtattaga cgaatcttct aaagtaattt caggactatc
     1441 aaaccacaca ggtattgtga ttgcacctaa ataccaatac agcattaagc acatagaatt
     1501 tatacgtctt aatagcagtc aggtaatgtc tattattgct agtgcgaatg gtcagataga
     1561 aaatagaatt attga
//
LOCUS       DTVV01000472            3182 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2426054, whole genome shotgun sequence.
ACCESSION   DTVV01000472 DTVV01000000
VERSION     DTVV01000472.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2426054"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="EYO86_05650"
     CDS             <1..201
                     /locus_tag="EYO86_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HIC42548.1"
                     /translation="KLGEEVAAAIVLKTKHQLTEQELKEFLRNKLAPFKIPQTIIFLD
                     EIPKGKTGKLQRLGLAKTLGLE"
     gene            201..1169
                     /locus_tag="EYO86_05655"
     CDS             201..1169
                     /locus_tag="EYO86_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011568409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIC42549.1"
                     /translation="MKICIVGAGATGGYLGVKLINAGFDVSLVARGAHLTAMKKKGLT
                     LIENDKEITCSPKCSDSMKELGKMDFIFITLKAYSINGLVEEISTMFDENTSVISSYN
                     GIPWWYFFGAEGQFKNYRIKCIDPKNIQWNVITPERIIGCVVYPATEIIEPGVIKHIE
                     GNRFSLGEPNGAQTERISRISKAMARADLKAPVRKNIRQEIWMKLIGNLAFNPLSVIT
                     GETLDVLLLNEENKKTAYEAMKEASSIMDKLNVPMSISIDQRIEGAAKVGSHKTSMLQ
                     DYERGKELELDALVVAVKEIADLLGIKTPTIDRILHTVTEKISKNN"
     gene            1174..1989
                     /locus_tag="EYO86_05660"
     CDS             1174..1989
                     /locus_tag="EYO86_05660"
                     /inference="COORDINATES: protein motif:HMM:PF01925.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIC42550.1"
                     /translation="MELEFLYFLVSLLITGIFSGLIAGLLGVGGGLIVIPVVFYILNF
                     YNFSKDIIMHVAIASSLGVIFITSLSSMYAHYKLNNIDANVVKKWFIGVVFGSIFGAI
                     FASSIDGNILVVIFGAIASIVAMSMLLNINIVLTNDIPRNFFLNNSISFLIGCFSVLI
                     GIGGGSFSVPILTAFGKEIHRAVGTSACIGFLIALPGFITYVSTGWVIEGLPKYSLGY
                     VSVPIVLTVASVSVLTAPLGAKLSNRANKNTLKKIFAIFLLLVCISLVIDQFY"
     gene            2001..>3182
                     /locus_tag="EYO86_05665"
     CDS             2001..>3182
                     /locus_tag="EYO86_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIC42551.1"
                     /translation="MNDILSQRLKKIQPSLTVSINVKANALRAEGRDVLVLAAGEPDF
                     DTPQNICQAAIKAMNEGQTRYVPGKGTPALQQAIVAKFKRDNNLSYNLDEIMVGVGGK
                     HIIYNAMMATLNPGDEVIIPAPFWVSYPDIVMLAEGKPIIVKCDASQDFKITPEQLEN
                     NITNKTKWLMLNSPSNPTGAVYSFNELKALSEVLLKCPNVLVLTDDIYEKIIYDDGVF
                     STIASVEPKMKERTLTLNGVSKAYCMTGWRLGYCGGPKEIIAGMNKIQSQSTTSTSSI
                     SMAASVEALSGDQEFINLHNNQFKKRRDMVVENLNQIDGINCSIPPGAFYVYPSCEGI
                     IGKKTSAGNTINTDKDFMNFLLESEGVAGVHGEAFGLSPYFRLSYATDEKTLADACNR
                     IE"
BASE COUNT         1043 a          474 c          644 g         1021 t
ORIGIN      
        1 aaacttggcg aagaagttgc agcggctatt gtcttgaaaa ctaaacatca attaaccgaa
       61 caagaactta aggaatttct tcgaaataaa ttggcaccct ttaagattcc ccaaactatt
      121 atttttttag atgaaatacc aaaaggaaaa acaggcaaac ttcaacgcct aggtttggct
      181 aaaacgctgg gattagaata atgaaaattt gtatcgtggg agcgggagca acgggagggt
      241 atttgggagt taagttgatt aacgcaggtt ttgatgtttc ccttgttgca agaggcgctc
      301 atttaacagc aatgaaaaag aaaggactta cccttataga aaatgacaaa gaaattactt
      361 gttctcctaa atgctcggat tctatgaaag aattaggaaa gatggatttt atttttatta
      421 ctcttaaagc ttactcaatt aatggattag tagaggaaat ttccacaatg tttgatgaaa
      481 atacttcagt aatatcatct tataatggaa ttccttggtg gtattttttt ggcgctgagg
      541 gtcaatttaa aaattatcgt attaaatgta ttgatccaaa aaacattcag tggaatgtaa
      601 ttacacctga gagaattata gggtgtgttg tttatccagc aactgagatt atagaaccag
      661 gtgttataaa gcacattgaa ggtaatcgat tttcactagg tgaaccaaat ggagcacaaa
      721 ctgaaagaat ttcaagaata agcaaggcta tggccagagc agaccttaaa gctccagttc
      781 gaaagaatat aaggcaagaa atttggatga aacttattgg caatctcgca tttaatcctt
      841 taagtgtcat tacaggagaa acacttgatg ttttactgct caatgaagaa aataaaaaaa
      901 cagcatatga agctatgaaa gaagcaagtt caataatgga taaattaaat gttccaatga
      961 gtattagtat tgatcagcgg atagaggggg ctgctaaagt tggatctcac aaaacatcca
     1021 tgttgcagga ttatgaaagg ggtaaggaat tggaattgga tgctcttgta gtagcagtga
     1081 aagaaatagc tgatctacta ggcattaaaa ccccaactat tgatagaatt ctacacacag
     1141 ttacagaaaa gatttcaaaa aataactagt ttcatggaat tagaattttt atattttcta
     1201 gtttctttgt taatcacggg tattttttca ggattaattg ctggcttatt aggagttggg
     1261 ggagggctca ttgttattcc agttgtgttt tatatcttga atttttataa tttttcaaaa
     1321 gatattataa tgcatgttgc aatagcatca tcacttggcg ttatctttat tacttcttta
     1381 tcatcaatgt atgctcatta taaactaaat aatattgatg cgaatgtcgt aaaaaaatgg
     1441 tttatcggag ttgttttcgg ctccattttt ggtgcaattt ttgctagttc catcgatgga
     1501 aatatattgg tcgttatttt tggagctata gcaagtattg tcgcaatgag tatgctttta
     1561 aatataaata tagttttaac aaatgacata cctcggaatt tttttttaaa taattccata
     1621 agtttcttga ttggatgttt ttcagtattg attggtattg gaggtggaag ttttagcgta
     1681 cctattttga ctgctttcgg aaaagaaata caccgtgctg ttggaacttc agcctgcatt
     1741 ggttttttaa ttgcccttcc aggttttatt acctacgtat caacaggatg ggtgattgaa
     1801 ggactcccaa agtactcttt aggatatgta agcgtgccca tcgttttaac tgtcgcatct
     1861 gtaagtgtat tgactgcacc attaggagcc aagctttcaa atagagctaa taaaaacaca
     1921 ctaaaaaaga tttttgctat ttttttattg ctcgtatgca taagtcttgt aattgatcaa
     1981 ttttattaaa attatatgaa atgaatgata ttttatccca aagattaaaa aaaattcaac
     2041 catccttgac ggtttcaata aacgttaaag ccaatgctct gcgtgctgaa ggtcgggatg
     2101 tattagtttt agccgctggt gagccagatt ttgacactcc tcaaaatatt tgtcaggcag
     2161 caattaaggc tatgaatgaa ggacaaactc gttatgtacc tgggaagggg acgccagcct
     2221 tacagcaggc aatcgttgct aagtttaaac gcgataataa tttatcctat aatttggatg
     2281 aaatcatggt tggggtagga ggtaagcata taatttataa tgcaatgatg gcaacgttaa
     2341 accctggtga tgaagttatc attcctgctc ctttttgggt cagttaccca gacattgtta
     2401 tgcttgcgga aggaaagccc ataattgtta aatgtgatgc gtcacaggat tttaaaataa
     2461 ctccagaaca acttgaaaac aatataacta ataaaactaa gtggctcatg cttaacagtc
     2521 caagcaaccc gactggagca gtttattcat tcaatgaatt gaaggcatta agtgaagtat
     2581 tgttgaaatg tcccaacgtg ctagttttaa cggacgatat atatgagaaa attatttatg
     2641 atgatggtgt attttctacc atcgcatccg ttgagcctaa aatgaaagag agaactttaa
     2701 ctcttaatgg cgtttcaaaa gcttactgta tgacaggttg gcgacttggt tattgtggag
     2761 ggcctaagga aattattgct ggcatgaata aaatacagtc acaaagcacc acatcaacgt
     2821 cttctatttc aatggcagct tcggttgagg cattatcagg tgaccaagaa ttcattaatc
     2881 tacataataa tcaatttaaa aaaagacggg atatggtcgt tgaaaattta aatcaaattg
     2941 atggtataaa ttgttcaatt cccccagggg cattttatgt atatcctagt tgtgaaggga
     3001 ttattggtaa aaaaacatct gctggcaata ctataaatac tgataaagat tttatgaact
     3061 ttttgttgga aagtgaaggt gtggcaggtg tgcatgggga ggcatttgga ttgtcaccgt
     3121 atttccggct ttcctatgct acggatgaaa aaactttagc agatgcctgc aatcgtattg
     3181 aa
//
LOCUS       DTVV01000473            1599 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2429049, whole genome shotgun sequence.
ACCESSION   DTVV01000473 DTVV01000000
VERSION     DTVV01000473.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2429049"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            402..971
                     /locus_tag="EYO86_05670"
     CDS             402..971
                     /locus_tag="EYO86_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42552.1"
                     /translation="MSESDDLFIDPSATVNQLINIIENRYQWSIDTDFQDKNNTYLFW
                     YVSEEKLEPRLGERYNEQGAELEQHLGIGKMVSELYHFLKENSAETKNLTIAKFLLIH
                     PEYRGIIRRIQTLTNYPFGEVSDNILAKNILPLNMLRFKLSFFGASRYDPKSDRWLRV
                     SFFAGAPFYEELNSQNVEDWGFATMNSYQ"
     gene            977..1588
                     /locus_tag="EYO86_05675"
     CDS             977..1588
                     /locus_tag="EYO86_05675"
                     /inference="COORDINATES: protein motif:HMM:PF12525.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3726 domain-containing protein"
                     /protein_id="HIC42553.1"
                     /translation="MSFSYYEVYTTSLRAFSSMGFPYGADEDAAYIIAWLELNKFNGL
                     NSLVNSIEVVDNKYDGKINPNALNNSSLDLNYSSLLMKGPGLIDFYQSELGDKKDLHV
                     TLNNCPNPEYLLPLLYRSSKKIYYSNAIWLDTNNQIQFCEVIKNIMKIGKVSNQELVD
                     KKQVRIFFSHEHKIQKKFDSVTCTITSKTIQDNLIRAWPRRWL"
BASE COUNT          571 a          265 c          261 g          502 t
ORIGIN      
        1 cttaccattc tcatgcacat catagacccc tccagtaaca ggtttattcg ttgattttag
       61 aagtagaatt tctggataat caatataatt agtttcatca acgtggtatc tattttgacc
      121 aataacactt gaagtcaaac aaagcaccaa aaacacgcat agatatttca taagactgtt
      181 tttctctaat ataataccaa taataaaata aacaaaataa agtgttattt taaaatgata
      241 aattcctatg taaatcaata aaaacatctg atttaaaaga tacaagatgg aatgacctta
      301 ttaaattttc tgaagccaat ttaagttttg acgcgcagga aatagttaag gttcaaatta
      361 ttgaattgtt tccagaaatt gctgatccac tcgcggagaa catgtcagaa tcagatgatt
      421 tgttcattga tcctagcgca acagtgaatc aattgatcaa tattatagaa aatcgatatc
      481 aatggtccat tgacaccgat ttccaggata aaaataatac ttatttgttt tggtacgtat
      541 ctgaggaaaa actcgaaccc cgcttgggag agagatacaa cgaacaagga gctgaattag
      601 aacaacatct aggcattggc aaaatggtaa gtgaacttta tcattttcta aaagaaaata
      661 gtgcggaaac aaaaaattta accattgcaa agtttttact tattcatcct gaatatcggg
      721 gcattatcag gcgtatacaa acacttacaa attatccttt tggtgaagta agtgataata
      781 ttcttgccaa aaatatcctt cctttaaata tgctaagatt caaattgtca ttttttggag
      841 cgtcacgtta cgatccaaaa tcagatagat ggttgcgtgt aagttttttt gcaggcgcgc
      901 ccttttatga agaactgaac tctcagaatg ttgaggattg gggttttgcg acaatgaatt
      961 cctaccaata aaagttatga gcttttctta ttatgaagtt tacacaactt cccttcgcgc
     1021 tttctcgagt atgggattcc cctatggtgc tgatgaggat gcagcttata taatagcatg
     1081 gcttgagcta aataaattca atgggctaaa ttccttggta aactcaattg aagtagttga
     1141 taataaatat gacggcaaaa taaatccaaa tgcactgaat aattcaagtt tagatttgaa
     1201 ttactcctcg cttttaatga aaggacctgg cttaattgat ttttatcaat ccgaattggg
     1261 tgataaaaaa gatttacatg ttacgctcaa taattgccct aatccagaat atttattacc
     1321 tcttttgtat agatcctcaa aaaaaatata ttacagtaac gccatctggc ttgacactaa
     1381 caatcaaatt caattttgtg aagttataaa aaatatcatg aaaattggca aagtatcaaa
     1441 tcaagaatta gttgataaga aacaagtaag gatttttttt tcacatgaac acaagattca
     1501 aaaaaaattt gattctgtca cttgcacaat cacttcaaaa acaattcagg ataatctgat
     1561 acgagcttgg cctcgccgat ggctttaagc agaaccagc
//
LOCUS       DTVV01000474            3314 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2429418, whole genome shotgun sequence.
ACCESSION   DTVV01000474 DTVV01000000
VERSION     DTVV01000474.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2429418"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="EYO86_05680"
     CDS             complement(<1..246)
                     /locus_tag="EYO86_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIC42554.1"
                     /translation="MISLIGFSGLILSFIFNVIVIVSYYYKINQRFNHIFYLALYCSL
                     FFMCLSFFALMFAYISSDFSNFNVFQNSHSSKPLLYKI"
     gene            complement(243..683)
                     /gene="ccmE"
                     /locus_tag="EYO86_05685"
     CDS             complement(243..683)
                     /gene="ccmE"
                     /locus_tag="EYO86_05685"
                     /inference="COORDINATES: protein motif:HMM:PRK13254.1-1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIC42555.1"
                     /translation="MTIRFQRLLLILFSLIFLSSAILLILFNSKKNLVFFFTPSELIN
                     SDSTINDVVRIGGFVEKDSIINKGNNSYNFVITDNNNKVLVEYNGLLPDLFKEEQGVV
                     IEGRLKSDFTIHAFTVFAKHDENYMPASIQKQLQKNQYWKKNYK"
     gene            complement(691..789)
                     /gene="ccmD"
                     /locus_tag="EYO86_05690"
     CDS             complement(691..789)
                     /gene="ccmD"
                     /locus_tag="EYO86_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIC42556.1"
                     /translation="MYFWYVFLSYAICFVLLLILILHSYLKLKKNK"
     gene            complement(853..1548)
                     /locus_tag="EYO86_05695"
     CDS             complement(853..1548)
                     /locus_tag="EYO86_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020021654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HIC42557.1"
                     /translation="MKFLINPNRFNQISNIMLTPLIILSVIALTTGLVFAFYLSPNDY
                     QQGSTVRIMYVHVPSAWLALLTFFIMTVYSIIGLAFKTPFSFIVNAAVAPIGATFTLI
                     CLITGSLWGKPMWGTWWVWDARLTSVAILFMLYLIIIFLKNSFTNDSLGEKITAIFII
                     VGSINLPIIKFSVDWWNTLHQPASISKLSSPSIDPSMMRPLIIMTIAFTLIALIIAII
                     RIKAEISERKLKN"
     gene            complement(1549..2223)
                     /locus_tag="EYO86_05700"
     CDS             complement(1549..2223)
                     /locus_tag="EYO86_05700"
                     /inference="COORDINATES: protein motif:HMM:PF03379.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42558.1"
                     /translation="MHSVNSILALFYRELKISYRNFSDILSILMFFLLGILIFVFSIG
                     PNTEIFNEIGIGVIWTLILLSNNLSLRKFYQNDFNDGSIILLHMSGLSYELIVLIKIM
                     IIWIFLQLPFFIIIPLAAILLNIELPNVNLILISFLIGSPILTSIASISGSMNLLNQR
                     NFAIGSVIIMILSIPIIIFSVGIINASEEIVRAQLQILIGILFFFLAITPWVSAACIK
                     LAIQNK"
     gene            complement(2210..2833)
                     /gene="ccmA"
                     /locus_tag="EYO86_05705"
     CDS             complement(2210..2833)
                     /gene="ccmA"
                     /locus_tag="EYO86_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIC42559.1"
                     /translation="MLLANNISFYRNNNIIFKDVSLALPPQKIINITGANGIGKTTLL
                     KILTHVLIPKKGDIFWNGKNIKKNLFNYYKDVTFVMDKQTSNINLSVIENIFFWKKLF
                     SSIISKKEIDAILDLLSLDSYRNTPINYLSNGEIKKLELTRLVIERKKLWMLDEPYIS
                     LDIETINLLNETFINHTKSGGMIIFSSHYVPDIPNIENLQLENYAQR"
BASE COUNT         1238 a          468 c          497 g         1111 t
ORIGIN      
        1 aattttgtat aacaaaggtt tagaagagtg tgaattttga aaaacattaa agtttgaaaa
       61 atcagaactt atgtatgcaa acattaatgc aaagaatgat aggcacataa aaaaaagact
      121 gcaataaagt gctaaataaa aaatatgatt aaaacgttga ttgattttgt agtagtatga
      181 aacgataaca ataacattaa agataaatga taatattagt ccactaaacc caatcaatga
      241 aatcatttgt aattcttttt ccaatactga tttttttgaa gttgtttttg aatagatgct
      301 ggcatgtaat tttcatcatg ttttgcaaaa actgtaaaag catgaatggt gaaatctgac
      361 ttaagccttc cctcaattac aacaccttgc tcttctttaa ataaatcagg aagtaatccg
      421 ttatattcaa ccaaaacttt attgttatta tcagtgatta caaaattata tgaattattt
      481 cctttattta ttatagaatc tttttcaaca aaacccccta ttctcacaac atcattaatg
      541 gtagagtcag aatttattag ttctgatgga gtgaaaaaaa aaaccaagtt ttttttagaa
      601 ttaaataata ttaacaaaat agcgctactt aaaaaaataa gtgaaaaaag aataagcaat
      661 agacgttgaa aacgaatggt catgtttaat ttacttattt tttttcaatt ttaagtatga
      721 atgcaaaatt agtattaata aaaggacaaa acaaattgca taagataaaa aaacatacca
      781 aaaatacata ctaccctaat atcaaatatt aattattata atagataatc cgacgagacg
      841 tatggcccca aattaatttt ttagctttct ttctgagatt tcagctttaa ttctaataat
      901 agcaattatt agggcaataa gggtaaaggc gatggtcata attatgagag gacgcatcat
      961 actggggtca atagaggggg atgaaagttt gctgatgctg gctggttgat gaagtgtatt
     1021 ccaccaatca acagaaaatt ttatgattgg taaattaata gatcccacta tgataaatat
     1081 cgcagtaatt ttttctccca aactgtcatt agtaaatgaa ttttttaaaa aaattatgat
     1141 cagatacagc ataaataaaa tagctactga tgttaagcgt gcatcccata cccaccaagt
     1201 gccccacatg ggtttacccc acaacgaacc tgtaattaag cagattaaag taaaagttgc
     1261 accaataggg gcgactgctg cgttaacaat gaaactaaaa ggagttttga atgcaagtcc
     1321 aatgatgctg tatactgtca taataaaaaa agttaacaaa gcaagccaag cagatggtac
     1381 atgaacatac atgatacgta cggttgagcc ttgttgatag tcattaggcg acaaataaaa
     1441 agcgaatacc aatcctgtag ttaaagcaat gacactcaga ataatgagag gtgtcaacat
     1501 aatgttgcta atttgattaa agcgatttgg atttatgagg aatttcattt atttattttg
     1561 aatagctaat ttaatgcaag cggcagaaac ccaaggtgtg atagctaaaa aaaagaataa
     1621 tattcctatt aggatttgta gttgagccct aactatttct tctgaggcat taatgatgcc
     1681 aacactaaaa ataattattg gtatgcttaa tatcattatg attacacttc ctatggcaaa
     1741 atttctttga tttaataaat tcatagatcc agaaatagaa gctatagatg ttaatatagg
     1801 cgatccaatt aaaaaactta taagaattaa gtttacatta gggagctcta tattcagtaa
     1861 aatagcagcg agagggatga tgataaaaaa aggcaactga agaaatatcc aaataatcat
     1921 tattttaatc aacactatta attcataaga aagtccgctc atatgtaaca aaataatact
     1981 tccgtcgtta aaatcatttt ggtaaaattt tcttaaagat agattattac ttaagagaat
     2041 tagtgtccag ataacaccta taccaatttc gttaaaaatt tctgtatttg ggccaataga
     2101 aaatacaaaa attaaaatgc ctaaaagaaa aaacataaga atacttaaaa tatcagaaaa
     2161 atttcggtac gatattttaa gttctctata aaaaagcgcc aatatagaat taacgctgtg
     2221 cataattttc caattgtaag ttttctatat taggaatatc aggaacataa tgggaggaaa
     2281 aaataatcat tccacctgat tttgtgtgat tgataaatgt ttcatttagc aaatttattg
     2341 tttctatgtc caaacttatg tatggctcat ctagcatcca taattttttt cgctctatta
     2401 ccaatcgcgt gagctctaat ttttttatct ctccgttaga taaataattt attggagtat
     2461 tacgataact atcaagggac agtaaatcta aaatagcatc tatttctttt ttactaatta
     2521 tggatgaaaa aagctttttc caaaaaaaaa tattttcaat aacagataaa tttatattag
     2581 aggtttgttt atccatgaca aatgtgacat ctttataata attaaaaaga ttttttttta
     2641 tgtttttgcc attccaaaag atatcacctt ttttcggtat taaaacatgg gttaaaattt
     2701 ttagaagagt tgttttgcct atcccatttg caccggttat attaattatc ttttgaggag
     2761 gtaaggctaa actcacatct ttaaatatga tattgttgtt tctataaaag gaaatattat
     2821 tagcgagcag cattatcttg tttaataaag acttaaatca gaagagacaa taataaaaaa
     2881 acggggcgat aaccccgttt ttgagattga aaaacctcta tttttaagaa aaagaaaact
     2941 tacttgaagc tttctcttaa ggagaaagaa actaccttct tcttttagca gcctttttct
     3001 tcttaggtgc agcttttttt cttttaggtg cagctttttt tcttttaggt gcagcttttc
     3061 tttttcttgg tgcagctctt ttctttgcca ctttcttttt cttctttgcc atttgatgct
     3121 ccttgtaatg ggtttaagtg gaggaacctc cctccatcat cacttaaatt aagtgataaa
     3181 cataaaaggc tggttattct tcccaaaact tacgctcgaa gtcaatgata tacttgtttt
     3241 tgaaattaaa gggtgagcaa attagaaata aatataatta ttaaacataa attaatattt
     3301 aaacgaataa aaaa
//
LOCUS       DTVV01000475            1035 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2431156, whole genome shotgun sequence.
ACCESSION   DTVV01000475 DTVV01000000
VERSION     DTVV01000475.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2431156"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="EYO86_05710"
     CDS             complement(<1..206)
                     /locus_tag="EYO86_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42560.1"
                     /translation="MFTKILKISFFLILFLISSCGIKRSWEEGGKYTSDKSLPSTYIW
                     KKGKQYIAIVQTIPVRGRNKKNTI"
     gene            278..>1035
                     /locus_tag="EYO86_05715"
     CDS             278..>1035
                     /locus_tag="EYO86_05715"
                     /inference="COORDINATES: protein motif:HMM:PF00494.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HIC42561.1"
                     /translation="MDSIETTITKNHKQENFPVGSWLLSKKIRSKILIFYKFARAADD
                     IADSPNLTSTEKIKRLNLFKKAIESNKIKISKAEDLRKICIKNKIRINHALNLLKAFK
                     QDAVKKRYKNWSELIRYCKFSAVPVGRFVIDLHKEKQKAYKYSDPLCIALQILNHLQD
                     CKEDYENLDRVYLPMQFLKKYNVKLSQLKKNVTEKNLRLVFNEILKNTEKLIIEAGKN
                     KKNMKHKHLSLETSFILEIAKKLLQLLKNNDPLK"
BASE COUNT          455 a          109 c          122 g          349 t
ORIGIN      
        1 tgaattgtat tttttttatt tctacctcta actgggattg tttgaacaat tgctatatat
       61 tgctttcctt ttttccatat ataggttgat ggtaatgatt tatcagacgt atatttacca
      121 ccttcttccc aagatctttt tattccacat gagctaatga ggaataaaat taaaaaaaaa
      181 gaaattttta aaatttttgt aaacattata aaattataaa aaaaagaata tattattata
      241 taatttgcat attttaaagg aatgaaactt tatatctatg gactcaattg aaactacaat
      301 aactaaaaac cataaacaag aaaactttcc tgtaggttct tggttactat caaaaaaaat
      361 taggtctaaa atactaattt tttataaatt tgcaagagca gcagatgata ttgctgatag
      421 tcccaattta acctcaactg aaaaaataaa aagattgaat ttatttaaaa aggctattga
      481 aagtaataaa ataaaaattt ctaaagcaga agatttaaga aaaatatgta taaaaaacaa
      541 aattagaata aaccatgcat taaatttatt aaaggcattt aaacaggatg cagtaaaaaa
      601 aagatataaa aattggtcag aattaattag atattgtaaa ttctcagctg ttcctgttgg
      661 tagatttgtg atagatttac ataaagaaaa acaaaaagca tacaaatatt cagatccttt
      721 gtgtatagct cttcaaattt taaatcattt gcaggattgt aaagaagatt atgaaaattt
      781 agatcgcgtt tatttaccta tgcaattttt aaaaaaatat aatgtaaaac tatctcaatt
      841 aaaaaaaaat gttactgaaa aaaacttaag gttggtgttt aatgaaattt taaaaaatac
      901 agaaaaatta attattgaag caggtaaaaa taaaaaaaat atgaagcata aacatttatc
      961 attagaaacc tcattcatat tagaaattgc aaaaaaatta ttacaattat taaaaaataa
     1021 tgatccttta aaaaa
//
LOCUS       DTVV01000476            1008 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2435474, whole genome shotgun sequence.
ACCESSION   DTVV01000476 DTVV01000000
VERSION     DTVV01000476.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2435474"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="EYO86_05720"
     CDS             complement(<1..922)
                     /locus_tag="EYO86_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIC42562.1"
                     /translation="MNTSQKLVYDSSNIKILEGLEAVRKRPAMYIGGTGIDGLHHLVF
                     ELVDNSVDEAIAGYCSEVEVILHIDGTVSVADNGRGIPVDIHSDRNISAAEVVMTVLH
                     AGGKFDKESYKVSAGLHGVGVSVVNALAETLSLEIKRDGKIHTQKYSRGKPVTGLEVV
                     GKTSTTGTKIVFNPDIEIFEDLNFRFEILSNRLRELAFLNKGLKIHIHDERDGKDNEF
                     LFEGGILSFVEHLGKNKKNIHQDPIYIERSKDHCDLEMALQYNDGYGEEIFTFVNNVN
                     TRDGGTHLSGFKSALTRTINGYASQNNLLKN"
BASE COUNT          296 a          226 c          152 g          334 t
ORIGIN      
        1 aattttttag caggttgttc tggcttgcat aaccgttaat tgttcttgtt aaggctgact
       61 taaaaccgct taaatgggtg cccccatccc gagtattgac attattgaca aaagtaaaaa
      121 tctcttcgcc ataaccatcg ttatattgga gcgccatttc taaatcacaa tggtctttac
      181 tccgttcaat atagattgga tcttgatgga tgtttttttt gttctttcct aaatgctcta
      241 caaaagaaag tattccacct tcaaataaaa actcattgtc tttcccgtct ctctcatcat
      301 gaatatgaat tttcagccct ttatttaaaa aagcgagttc ccgaagacgg ttagataaaa
      361 tctcaaaccg gaagtttaaa tcttcaaaaa tttctatatc tggattaaaa acaatctttg
      421 tacccgttgt gctggttttt cctacaactt ccaagcctgt gacgggttta ccccggctat
      481 atttttgagt atgaattttt ccatctctct ttatttccaa gctcaatgtt tctgctaaag
      541 cattgaccac cgatacccca acaccgtgca accctgccga tactttatag ctctctttat
      601 caaacttccc tccagcatga aggaccgtca tcaccacttc tgcggccgaa atattcctat
      661 ctgagtgtat atctacagga attccccggc cattatcggc aaccgaaacc gtaccatcaa
      721 tatgtaaaat aacttctact tctgagcaat aacccgcaat ggcttcgtct acactattat
      781 ccaccaattc aaaaaccaag tgatgtaagc catctatacc tgtcccacct atatacatgg
      841 caggtctttt tcgaacagct tctaatcctt ctagaatttt aatatttgag gaatcgtata
      901 caagtttttg agatgtattc atatatgctt aatagtaact ttataaaaaa aacttatatt
      961 tatttatagt agtagtagta gggcctgaaa gcatgtggaa aacctggc
//
LOCUS       DTVV01000477            1127 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2435520, whole genome shotgun sequence.
ACCESSION   DTVV01000477 DTVV01000000
VERSION     DTVV01000477.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2435520"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="EYO86_05725"
     CDS             <1..587
                     /locus_tag="EYO86_05725"
                     /inference="COORDINATES: protein motif:HMM:PF13561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC42563.1"
                     /translation="SEYIVCDINNIKEFEKKTSSIKRINTYINNAGINIPKHFLKVNL
                     NDLDVMVNLNLRSLFLTTQVIVKKMIKYKNISRSNSSIINISSTLGHVGSHNRTVYCM
                     TKFGVEGFTKALAIDLAKYKIRVNAIAPTAIMTPMLKKYYSKDFEKKTIPNILLNRIG
                     RVDDVASTAVFLASNAAKFITGTSLLVDGGWTAK"
     gene            complement(594..>1127)
                     /locus_tag="EYO86_05730"
     CDS             complement(594..>1127)
                     /locus_tag="EYO86_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase"
                     /protein_id="HIC42564.1"
                     /translation="VADANTGWLMHDALRVIKATEHLDIYYEQPCASYEESLAIRKKT
                     KNPFILDENIDSIHSFLRAYHDQAMDIINIKISKLGGVTKSRLLRDLCVQLGIAMTIE
                     DSWGGDIATAAIAHLSHSTPEKFRFSSTDFNNYNTFSYSKGSPQSKNGKFSASDNPGL
                     GVILDDKLIGEPEFTII"
BASE COUNT          400 a          180 c          171 g          376 t
ORIGIN      
        1 aatcagaata tattgtctgt gatattaata atatcaaaga atttgaaaaa aaaacttcct
       61 caattaagag aatcaatact tatatcaaca atgcaggaat caatatcccc aaacattttc
      121 taaaagtaaa tttgaatgat ttagatgtta tggttaattt gaatttacgg tcattatttt
      181 tgactaccca agtaattgtt aaaaaaatga taaaatataa aaacatatca agaagcaatt
      241 caagtataat aaatatctct tccactttag gtcatgttgg ttctcataat cgaactgttt
      301 attgtatgac aaaatttggc gtagaaggtt ttactaaagc tttggcaatt gatttagcaa
      361 aatacaaaat tcgcgtcaat gcaattgcac caacagcaat tatgacacca atgttgaaaa
      421 aatattacag taaagatttt gaaaaaaaaa caataccaaa tattttgcta aatcgcattg
      481 gtagagtgga tgacgttgca tctacagctg tttttttagc ttcaaatgca gctaaattca
      541 taactggaac atctttactg gttgatggtg gttggacagc aaaataatat tacttatatt
      601 attgtaaatt cgggttcacc aattagttta tcatctaaaa ttacacctaa tccaggatta
      661 tctgaggctg aaaatttacc atttttactt tgtggtgaac ctttggaata agagaaagtg
      721 ttataattat taaaatcagt agacgaaaag cgaaatttct caggtgtgct gtgggaaaga
      781 tgtgcaatag ctgctgtagc aatatcacca ccccaggagt cttcaatagt catggcaata
      841 cctaattgaa cgcacaagtc tcttaaaagt ctactcttag taactccccc aagtttactt
      901 atcttaatat ttataatatc cattgcctga tcgtgataag ctctcaggaa tgaatgtatg
      961 gaatcaatat tttcatccaa tataaatgga tttttagttt ttttccttat ggctaaactt
     1021 tcttcataac tagcgcatgg ttgctcataa taaatatcaa gatgttctgt tgctttaata
     1081 actctcaaag catcatgcat tagccaccct gtattagcat ctgcaac
//
LOCUS       DTVV01000478            2715 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2440006, whole genome shotgun sequence.
ACCESSION   DTVV01000478 DTVV01000000
VERSION     DTVV01000478.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2440006"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            11..2689
                     /gene="rpoB"
                     /locus_tag="EYO86_05735"
     CDS             11..2689
                     /gene="rpoB"
                     /locus_tag="EYO86_05735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIC42565.1"
                     /translation="MGRTRFRKSFGHIKEILDMPNLIEVQRESYEKFLQSNTDANKRI
                     HFGLQQVLKEIFPIHDYSGKATLEFIKYKLDKPKYDVDECKQRGFTYASGLKVTLRLI
                     RYDIDEEDIEKKMFRDSVDDEVYLGDMPLMTERGTFVVNGVERVVVSQMHRSPGVFFD
                     HDNGKSHASGKVLYSARIIPMRGSWIDFEFDVKDFLYVRIDRKKKMLASTFFMACKKD
                     TKEKKDVSGMSPEEILGYFYETTKLVRRNDGWITTFDKAKFNNIKLTNNLINVKNKSV
                     IKKAGSKITLKEAEELEKNGLKEIFFFDKNLIGKYFAKDIINTKTGKIFFEAGDEIDE
                     KNFKLIEENNIENIEILNIDHVNVSAALRNTVHADLNINREEALIDIYRNLRPGEPPT
                     FEAAELMFHNMFFNPERYDLSKIGRYKLNNRNELNIDEEVNVITTEDVLKIVKNLLEL
                     NDRKGKKDDIDHLGNRRVRSVGELIENQYRIGLVRMERAIKERMHSVDIFTATPQDLI
                     NSKPAVAILREFFGTSQLSQFMDQTNPLSEITHKRRISALGPGGLTRERAGFEVRDVH
                     PTHYGRICPVETPEGPNIGLINSLATYSKINQYGLIESPYRKVVKEKVTDEVVFLSAD
                     DEQEYTIAQANTKVNENGEIVDKLVSSRKGGDFLMALPSQIDFMDVSPKQLVSVASSL
                     IPFLENDDANRALMGSNMMRQAVPLLKPSAPFIGTGMEASVGKDSGVTIVAKRNGIVD
                     SVDASRIVISAANPLQNESAVDIYNLKKFQRSNQSTCINQIPLVKKGDVIKSGEIIAD
                     GPATSKGELALGKNILVAFMPWQGYNFEDSIIMSERVVTDDIFTSVHIEEHEVCTRDT
                     KLGQEEITRDIPNTGDEALKNELLKCVFKLSFQNCF"
BASE COUNT         1052 a          337 c          494 g          832 t
ORIGIN      
        1 tacaaattta attggacgaa ctcgatttag aaaaagtttt ggtcatatca aagaaattct
       61 tgacatgcct aacctcattg aagttcaaag agaatcttat gaaaaatttc ttcaaagcaa
      121 tacagatgca aataaaagaa tacattttgg tcttcaacaa gttttaaaag aaatttttcc
      181 aattcatgat tattccggca aagccacatt ggaatttatt aaatataaac tcgataaacc
      241 aaaatatgat gttgatgaat gcaaacaaag aggttttacc tatgcatcag gtttaaaagt
      301 tactttgagg cttattcgat atgatattga tgaagaagat attgaaaaaa aaatgtttag
      361 agattctgtc gatgacgaag tttatttagg ggatatgccg ctaatgacgg aaagaggaac
      421 ttttgttgtt aatggtgttg aaagagtagt cgtttcacaa atgcatagat ctccaggcgt
      481 attttttgac catgataatg gaaaaagtca cgcatcaggt aaagtattat attctgcaag
      541 gataattcct atgagaggtt cttggataga tttcgaattt gacgtaaaag attttttata
      601 tgttcggatt gatcgtaaaa agaaaatgtt agcgagtacc ttttttatgg cttgcaagaa
      661 agatactaag gagaaaaaag atgtttcagg aatgtcgcca gaagaaatat taggatattt
      721 ttatgaaacc acaaaattag ttcgtaggaa tgatggttgg attaccacat ttgacaaagc
      781 taagtttaat aacattaagt taactaataa tttaattaat gtaaaaaata agtctgttat
      841 taaaaaagct ggatcaaaaa taactttaaa ggaagctgaa gaattagaaa aaaatggttt
      901 aaaagaaatt tttttcttcg ataaaaacct tattggcaaa tattttgcga aagacataat
      961 taataccaaa acaggaaaaa tattttttga ggctggtgat gaaatagacg aaaaaaattt
     1021 taaattaata gaagaaaata atattgaaaa tattgaaata cttaatattg atcatgttaa
     1081 tgttagtgct gcattaagaa atactgttca tgcagactta aatataaaca gagaagaggc
     1141 tcttattgat atatatagaa atctcagacc aggagagcca ccaacctttg aagctgctga
     1201 attaatgttt cataatatgt tttttaaccc tgaaagatat gacttatcaa aaattggcag
     1261 atataaatta aataatagaa atgaattaaa tattgatgaa gaagttaatg ttattacaac
     1321 tgaagatgtt ttaaaaatag taaaaaattt attagaatta aatgatagaa aaggtaaaaa
     1381 agatgatatt gatcatttag gcaacagacg tgttagatcg gttggagaat taattgaaaa
     1441 tcaatataga attggtcttg tcagaatgga acgtgctatt aaagaaagaa tgcactcggt
     1501 tgacatattt acagcaactc cacaagatct tataaattca aaaccagcag tggctatatt
     1561 aagagagttt tttggaacat cacaactgtc acagtttatg gaccaaacca atcctctttc
     1621 tgaaattact cataaaagaa gaatttctgc tttaggtcca ggaggactta caagagaaag
     1681 agctggcttc gaagtaagag atgttcatcc aacacattat ggtagaattt gtccagttga
     1741 aacaccagaa ggtccaaata taggattaat taattcatta gccacctact caaaaataaa
     1801 tcagtatggt ttaatagaaa gtccatatag aaaagtagtt aaagaaaaag ttacagacga
     1861 agtggtcttt ttatcagcag atgacgaaca agaatataca atagcacaag ctaatacaaa
     1921 agttaatgaa aatggagaaa ttgttgataa attagttagt tctagaaagg gaggtgactt
     1981 tttaatggca cttccatcgc agattgattt tatggacgta tcaccaaaac agctggtttc
     2041 tgttgcaagt tcgttaatac cttttttaga aaatgatgat gctaatagag ctttaatggg
     2101 atcgaatatg atgagacagg cagtaccatt acttaaacca tcagcaccat tcattgggac
     2161 aggaatggaa gcatctgttg gaaaagactc aggagttaca atagttgcta aaagaaatgg
     2221 tatagtagat tcagttgatg ctagtagaat tgttataagt gctgcaaatc ctttacaaaa
     2281 tgaatctgct gttgatatat acaatcttaa aaagtttcaa agatctaatc aaagtacttg
     2341 tataaaccaa attccattag ttaaaaaagg agatgtaata aaatcaggtg aaattattgc
     2401 tgatggacca gcaacttcaa aaggagaact tgctttaggt aaaaacattc ttgtagcttt
     2461 tatgccttgg caaggataca actttgaaga ctcaattatt atgtctgaaa gagtagttac
     2521 ggatgacatt tttacttctg ttcatataga agaacatgaa gtttgtacta gagatacaaa
     2581 attaggtcaa gaagaaatca ctagagatat acctaacact ggtgatgagg cattaaaaaa
     2641 tgaactttta aagtgcgttt tcaagctaag ttttcaaaat tgcttttgat atttgtgctt
     2701 aacagtttac ttaga
//
LOCUS       DTVV01000479            2212 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2441350, whole genome shotgun sequence.
ACCESSION   DTVV01000479 DTVV01000000
VERSION     DTVV01000479.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2441350"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(207..1505)
                     /locus_tag="EYO86_05740"
     CDS             complement(207..1505)
                     /locus_tag="EYO86_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011044272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIC42566.1"
                     /translation="MDLTTSYVGYISLSIFVLAYALVMIEEFSHLRKSKPVIISAALI
                     WGIIAFYFSSVPGLHSEKVEKALEHNILEFAKLFLFLLSAMTYINALQERNVFDVVRY
                     KLISRGFNFRQLFILTGVFTFFLSPIADNLTTALVMCSVLLACGKGNVKFLSLGCINI
                     VVAANAGGAFSPFGDITTLMVWQAGIVEFSAFFKLFIPSVVNYVIPAAIMYFFIPNEM
                     PNVSSEEIKIKRGGKVIIFLGLFTIFSAVSFHNFLHLPPMLGMMFGLGLLGLFSFYLK
                     VTYNEKYDEEKFDFLRKISRVEWDTLLFFFGVILSVGGLGFIGYLGLISNFLYVGLGA
                     TYANVLVGILSAIVDNIPVMFAVITMNPPMPTEQWLLVTLTTGVGGSLLSIGSAAGVA
                     LMGQARGHYTFFGHMKWTPVIFIGYIVSIYVYMLLSGLSL"
     gene            complement(1675..2055)
                     /locus_tag="EYO86_05745"
     CDS             complement(1675..2055)
                     /locus_tag="EYO86_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC42567.1"
                     /translation="MERAYRIKRYANEGWGVLLFAWRGYSGNKCYPTKNNLYKDAESV
                     LRWIEDYTDLQKSDLIVYGESLGTGIAVELGTRYLFKSIVLEAPFTSIADIAQKKYII
                     YPAKFFVLDKFNNFKKITKVFYPY"
BASE COUNT          820 a          397 c          290 g          705 t
ORIGIN      
        1 aatatttttt ttgtattttc tttttcagca gcactaagct ttggggattt cactatcata
       61 tcatttttta aaaatgtgaa ttttcaaacc ctcccaagtt tactatataa attagttatc
      121 tcttatagat ttacagatgc tacctttgtt gcaggattta tattaatctt tagtttattt
      181 atatacctgc tttttgataa taatatttac aaagacaaac cagataaaag catataaaca
      241 taaatgctaa ctatataacc aataaatatt actggggtcc atttcatgtg cccaaagaat
      301 gtataatgac ctctggcctg tcccatcaga gcaacacccg ctgctgagcc aatagatagc
      361 aaacttcctc ccactccagt agtcaatgta accaaaagcc attgctcagt tggcattggt
      421 ggattcattg taattaccgc aaacataaca ggaatattat cgacaatagc agataatata
      481 cctactaaca cattagcata agtagctcct aaacccacgt acagaaagtt agatataagg
      541 cccaaataac caataaaacc aagacctccc acacttaaaa ttacaccaaa gaaaaacagt
      601 aaagtgtccc actctactct tgaaattttt cttaaaaaat caaatttttc ttcatcatat
      661 ttttcattgt aagttacttt taaataaaaa gaaaataagc caagaagacc caaaccaaac
      721 atcattccaa gcataggtgg tagatgtaaa aaattatgga aactaacagc agaaaaaatt
      781 gtaaataaac ccaagaagat aattacttta ccccctctct ttattttaat ttcttctgaa
      841 gatacattgg gcatttcatt tggtataaaa aaatacatta ttgcagcagg aattacataa
      901 ttgacaacag aaggaataaa taatttaaag aatgctgaaa attcaacgat acctgcctgc
      961 cataccatta aagtagttat atcgccaaaa gggctaaagg ctcctccagc atttgcagca
     1021 actacaatgt taatacatcc aagtgataaa aatttaacat ttcccttgcc gcatgcaagt
     1081 aaaactgaac acattaccaa agccgttgtt aaattatccg ctataggtga caaaaaaaat
     1141 gtaaagactc ctgtcagaat aaataattga cgaaaattaa atcctcggga aattaactta
     1201 taccgaacta catcaaaaac atttctttct tgaagtgcat tgatgtaggt cattgcagaa
     1261 agaagaaata aaaatagttt agcaaactcc aaaatattat gttcaagggc tttttcaact
     1321 ttttcactat gtaacccagg gacagaacta aaataaaaag caatgatgcc ccaaataagc
     1381 gcagcagata taataacagg cttagatttt cttaaatgag aaaattcttc aatcataacc
     1441 aaagcataag ccaatacaaa tattgagagt gatatataac ctacgtaact tgttgttaaa
     1501 tccatatgta tttttatgag ttaaatttaa ttactttttt atcaatatca aaatcatata
     1561 aattattatg cattgcttcg ctaataaaaa gacattcttt tggatgattg gcttttgaaa
     1621 acagcttaac actatgagaa tgtggtatta cttcatcttt cattccacta ataatcaata
     1681 aggatagaaa acttttgtaa tttttttaaa attattaaat ttatctaaaa caaaaaattt
     1741 cgcgggataa attatatatt ttttctgggc tatatctgca atggatgtaa aaggagcttc
     1801 tagaactatt gacttgaata aataacgtgt acctaattct accgctattc ctgtacccaa
     1861 agattcccca taaacgatca aatcagattt ttgcagatct gtatagtctt ctatccatct
     1921 taggactgac tctgcatctt tgtataaatt atttttagtt ggataacatt tattaccact
     1981 atatccacgc caagcaaaca atagaactcc ccatccttca ttggcgtatc tcttaattct
     2041 atatgctctc tccaatatca aaagaatttc cttgcaaata aattaatgtt ggtttgttag
     2101 tttttgattt ggaaaaccaa gtaagtaacc caacatcatc agaggtgatt attttgatga
     2161 tttcaatatt atttaatcca taataagcaa gttcttgaga tatacctgag ac
//
LOCUS       DTVV01000480            1026 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2442956, whole genome shotgun sequence.
ACCESSION   DTVV01000480 DTVV01000000
VERSION     DTVV01000480.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2442956"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            17..>1026
                     /locus_tag="EYO86_05750"
     CDS             17..>1026
                     /locus_tag="EYO86_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIC42568.1"
                     /translation="MSSSGYSSESIKVLKGLEAVRKRPGMYIGDTDDGSGLHQMIYEV
                     VDNSIDEALAGFCTDISVFLNPDGTITVEDNGRGIPIEVHKTEGVSAAQLIMTELHAG
                     GKFDQNSYKVSGGLHGVGVSVVNALSEKLELEISRNGNLYFMEFTHGSVKTKLKQIGK
                     SKNIDSKNTATGTKITFLPSNKTFQNIEFNFNTIENRLRELAFLNTGVKLILLDKRSS
                     EEKKVEFKFEGGINEYVKYLNQSKNPIHSIPIYFNASKNNVNIECAIHWTDSYHENTL
                     CFTNNIAQRDGGTHLAGFRGALTRSIVNYINSKYKNNINITGDDAREGLTCVISVKLP
                     DP"
BASE COUNT          383 a          125 c          195 g          323 t
ORIGIN      
        1 taatataacc taatttatga gttcttctgg ttattcatct gagtctatta aggttttaaa
       61 ggggttggag gccgtaagaa aaagaccagg aatgtatatt ggcgatacag atgacgggtc
      121 tggcttgcat caaatgattt atgaagttgt ggataattct attgatgaag ctctagctgg
      181 attctgtacg gatatttcag ttttcttaaa tcctgacgga actattactg ttgaagataa
      241 tggaaggggc attcctattg aagttcataa aacggaagga gtttctgctg cacaattgat
      301 aatgactgaa ttacatgcag gaggaaaatt tgatcaaaat agctataaag tttcgggtgg
      361 attacatgga gtgggggtct ctgttgtgaa tgccctatca gaaaaattag aattagagat
      421 tagtagaaat gggaatttat attttatgga atttactcat gggtctgtta aaacaaaatt
      481 aaaacagatt ggaaaatcaa aaaatattga tagtaaaaat acagctactg gaacaaaaat
      541 aacattttta ccttcaaata aaacatttca aaatattgaa tttaatttta atacaattga
      601 aaatagatta cgcgagttag cttttttaaa tacaggggtt aagcttattt tactagacaa
      661 gcgttcttcc gaagaaaaaa aagtagaatt taaatttgaa ggtggaatta acgaatatgt
      721 taaatattta aatcaaagta aaaaccctat acattccata ccaatttatt ttaatgcaag
      781 taaaaataat gtgaatatag aatgcgcaat tcattggaca gatagttatc atgaaaatac
      841 tttatgtttt actaataaca tcgcccaaag agatgggggc acacacttag ctggatttag
      901 gggtgcctta acaagaagta tcgtaaacta tataaattca aagtataaaa ataatattaa
      961 tattacaggt gatgatgcta gagaaggatt aacatgtgtc atttctgtga aacttccaga
     1021 tccaaa
//
LOCUS       DTVV01000481            2636 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2444355, whole genome shotgun sequence.
ACCESSION   DTVV01000481 DTVV01000000
VERSION     DTVV01000481.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2444355"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="EYO86_05755"
     CDS             complement(<1..1098)
                     /locus_tag="EYO86_05755"
                     /inference="COORDINATES: protein motif:HMM:PF01266.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC42569.1"
                     /translation="MVAKKKKRTWSYYFYKLMMTKALIKDTPRIIVIGGGASGCGVAR
                     DLVLRGYQTTLIEFGDLGSGTSSRFHGMLQSGARYAVSDTSYAAECFRERETIAKIVP
                     EAVELIGGLFISLPNDPEDYPEKFSKCCEIAKIPYREIKIDEVLNKEKELNKNVLRAF
                     QVPDAIIHSWKLVNLLAKDIKAKGGEVLTNHQVVSIKQHNNLVKSVEVIEKNGVKKTL
                     ECDGIINASGPWSGKIAKLVDQEVELQLTKGSVMVFSHRLVSQAINRCRIPSSNDIMC
                     PSGTVSLWGTTSEIVNDPDTTKIRPEEIQELLMGAEELFPNVKNYRSFRAWAGVRPLV
                     KPKNFDKNMPLPRSHSVIDHQENGLRNFLTVC"
     gene            complement(1047..>2636)
                     /locus_tag="EYO86_05760"
     CDS             complement(1047..>2636)
                     /locus_tag="EYO86_05760"
                     /inference="COORDINATES: protein motif:HMM:PF00528.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC42570.1"
                     /translation="AISLLYVAFFDLWESTMTTFSSIIVAVIIGIIIGTFLGILSYKS
                     KTLNNILTPTFDFMQTMPVFAYLVPALMLFGYGPAAALVVTLIYAMPPMARVTKNALD
                     QVNSEIVELGKMVGCSTMQMLYRILLPSIRPRMMLGVNQVIMLTLNMVIIASMIGAGG
                     LGYNVWQSLKQLRIGQGFEAGIAITLVAIILDRLSQKIATRRPVYHEQKTKFFQKNIY
                     LIIIFLFIIITWLCGNWFSFFYNIPENMTVSSGRFWDGIIDYININYHGPIRLVRDFF
                     YIYLLKPFKMFMISIPWFVVVLLVFTIGLRIQGFALGLKCSILVLFIAMSGFWEKAMI
                     SVYLIGICVLLANLIGIPIGIIAASNDRVHRITQVIIDTFQTLPAFVYLIPVIMIFSV
                     GDFSAMIAVVLYAVVPAIRYTDNGIRQVSSSYLEAAKSTGCTKFQILRRVQLPLSLPD
                     IMLGVNQTLMMGLSMLIITALVGTRDLGQETLIALSKVDPGRGLTAGICVAFIAIIVD
                     RLINAWSQKRKKELGLTTSIS"
BASE COUNT          935 a          477 c          357 g          867 t
ORIGIN      
        1 acatacagtt aaaaaattcc ttaatccatt ttcttgatga tcgataaccg aatggcttcg
       61 aggaagaggc atatttttat caaaattttt tggtttaact agaggcctaa caccagccca
      121 agctctaaag gatcggtagt tttttacatt gggaaacaat tcttcagcac ccattaaaag
      181 ctcttgaatt tcttcagggc gtatttttgt tgtgtctgga tcattaacta tttctgaagt
      241 tgtaccccaa agactaacag ttcctgaagg acacataatg tcattgcttg aaggaattct
      301 acatctattt attgcttgac taacaagtcg atgtgaaaaa accattacag aaccttttgt
      361 aagctgtaat tcaacttctt gatcaactaa ttttgcaatt ttaccagacc acggacctga
      421 ggcattaatt atgccatcac attcaagtgt ttttttaacg ccatttttct ctattacttc
      481 aactgacttt accaaattat tatgttgttt tattgataca acttgatgat ttgtaagtac
      541 ttcaccacct tttgctttaa tatctttagc aagtaaattt actaatttcc aggaatggat
      601 aatagcatct ggaacttgaa aggctctcaa gacattttta tttaactctt tctctttatt
      661 taaaacttca tcaattttaa tttctcgata aggaattttt gcaatttcgc agcacttact
      721 gaatttttca ggataatctt cagggtcatt tggcaagctg atgaataaac caccaattaa
      781 ttcaactgct tctggtacaa ttttagctat agtttctctt tccctaaaac attcagcagc
      841 ataagaggta tctgaaacag cataccttgc accactttga agcatcccat gaaatctaga
      901 actagtacca gaacctaaat ctccaaattc aattaatgtt gtttgatatc ctcttaaaac
      961 taaatcccta gctacaccgc atcctgaagc cccaccacca attacaataa ttctaggagt
     1021 gtcttttatt aatgccttag tcatcatcaa cttatagaag tagtaagacc aagttctttt
     1081 tttctttttt gcgaccatgc attgataagt ctatcaacaa taattgcaat aaaggctaca
     1141 cagattccag ctgtaagacc tcgacctgga tcaactttgg atagagctat taatgtttct
     1201 tgacctagat ctcgtgttcc aactaaagct gtaataatta acatgcttaa tcccatcatt
     1261 aatgtttgat taacaccaag cataatatca ggaagagata atggtaattg aactcgacgt
     1321 aaaatttgga atttagtaca gccagtagat ttagctgctt ctaaataact tgatgacact
     1381 tgtcttatac cattatcagt atatcttatg gctggaacaa ccgcatagag aacaactgct
     1441 atcattgctg aaaaatcacc tacactaaaa atcattatta ctggtattaa atatacaaaa
     1501 gcaggtaaag tttgaaatgt atcaataatt acttgagtta ttcgatgaac tcgatcatta
     1561 cttgcagcaa taatgccaat aggaatacca attaaatttg ctaataaaac acagatgcca
     1621 attaaataaa ctgatatcat tgctttttcc caaaaaccac tcattgcaat aaacaagact
     1681 agtattgagc attttaatcc taaagcaaaa ccttgaattc ttaatcctat agtaaagact
     1741 aataatacaa ctacaaacca aggaattgat atcataaaca ttttgaaagg ttttaataaa
     1801 tatatataaa aaaaatcacg aactaatcta attggaccat ggtaattaat gttaatataa
     1861 tcaattattc catcccaaaa acgtcctgat gagactgtca tattttctgg tatattataa
     1921 aaaaaagaaa accaatttcc gcataaccaa gtgataataa taaataaaaa aattattatt
     1981 aaataaatat ttttttgaaa aaattttgtt ttttgttcat gataaactgg tcttcttgtt
     2041 gctatctttt gacttaatct atctaaaata atagcaacta aagtaattgc tattccagct
     2101 tcaaaacctt ggccaattct taattgtttt aaagattgcc atacattgta acctaggcca
     2161 ccagctccta tcattgaagc gataataacc atatttaaag ttaacattat tacttggtta
     2221 actcccaaca tcattcttgg acgaatagat ggtagtaaga ttctataaag catttgcata
     2281 gttgaacaac caaccatttt tcctaactca acaatttcac tattgacttg atctagtgca
     2341 ttcttcgtta ctcttgccat aggaggcatt gcataaatta aagttacaac caatgctgct
     2401 gcaggaccat atccaaataa cataagggca ggaactaaat atgcaaatac aggcatagtt
     2461 tgcataaaat caaaagttgg agttaatatg ttattaagag tttttgattt ataagaaagt
     2521 attccaagga aggtaccaat aataatacca ataataacag caacaataat tgaagaaaaa
     2581 gtagtcatag tactttccca taaatcaaaa aaagctacat ataataatga aatagc
//
LOCUS       DTVV01000482            2724 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2445148, whole genome shotgun sequence.
ACCESSION   DTVV01000482 DTVV01000000
VERSION     DTVV01000482.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2445148"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            742..1074
                     /locus_tag="EYO86_05765"
     CDS             742..1074
                     /locus_tag="EYO86_05765"
                     /inference="COORDINATES: protein motif:HMM:PF07345.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HIC42571.1"
                     /translation="MILFMNTFDEREKAFEAKFKQDEDVKFKLKAKRNKLLGKWAGET
                     LQKENLEEYIKEVRESNLVKPGDEDIIEKIMKDFSSNNKEITREELLQKFTECEKKAR
                     EELIEQNS"
     gene            complement(1109..2104)
                     /locus_tag="EYO86_05770"
     CDS             complement(1109..2104)
                     /locus_tag="EYO86_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stearoyl-CoA 9-desaturase"
                     /protein_id="HIC42572.1"
                     /translation="MKVDLNKWYKCDIDKQVYQNLIKRSDWQGIKHVSLWIIVLVFSG
                     YMAFINWGTWWAVFWFFVYGNIYMSSNPIWHDCGHRSAFKSRWLNEIFYQITSFMYSY
                     EPVRWRWSHFRHHSHTLSTDGPYDYEIQITKPTDLFYVLLMHIPGGNLLIIHKTFLFF
                     HLETIKHAIGIMTPVMEDCIPEKKRPSCRLFARIHVSIWLTIIIFSLILKSWLPTLYL
                     LLPFMYGTTFMQCIHFMQHAGLKNNIKDHRLCVRTVKLNPFFSFLNWNMEYHLEHHMF
                     PMVPAYNLKKLHDVLKEQMPPPKNGFWDAYKEIIPTIIKQSKNPNYILKVDLPTQ"
     gene            complement(2185..2625)
                     /locus_tag="EYO86_05775"
     CDS             complement(2185..2625)
                     /locus_tag="EYO86_05775"
                     /inference="COORDINATES: protein motif:HMM:PF00571.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIC42573.1"
                     /translation="MSKAMVGGLVTKLSKRKCFTLSEHDTLKSASEKLQKHNIGAMPV
                     VSKQNNDIIGIVSERDLARFIFKDEFKNDLPVTNIMTKEIITCNLNTSVTELMEIITQ
                     NKIRHIPIVEGKKILGIVSVGDVVNHVIEKYKDENKHLKDYMNL"
BASE COUNT          974 a          417 c          400 g          933 t
ORIGIN      
        1 aaaaattccc attaatatct ccaaacttaa aaatgttagc ggattaatca gagcatcttc
       61 tttattcaga aaatttaata tttcttaaga aaaaccccaa aaacatatca tgttgattgg
      121 ggtatatttc ggctcatcac ctaatgcgtg gcctgtgaaa caggtctgct ttatttgttt
      181 aaattatgtt ggttgtggcc accacccgtc agacaactag cggtattaat aataccctcc
      241 tatcttcatt tttaagctta tgcttttttc aatttatcgc aacagtcatt aagaaaaata
      301 atttacctaa atatggtgag aatgggggga aatatattta ttcatactaa aacaacagta
      361 acaataaaaa aaatagcaaa ataactaaaa gcactattat taggttattg ttttgattgc
      421 cgcgaataaa aattttatga caaactttac actcaatttt ttcagtcttg cctgatggtc
      481 caaatccaat ctttacttcc aacagttttg ttttggggtg ataattaaag taacaaaaaa
      541 caaatagtag tttttctaac atttaccttt tcactatata ttttctaaaa atgtaagtta
      601 atagataaat ataataaatg aataaatatt gatccaattt tacccttatt tttctaagtt
      661 cattacattc aatggatctt ggggataagt taatcaaata ccaatatttt attattcttt
      721 accagttaat aaaaagactc tataattttg tttatgaata cttttgatga aagagagaaa
      781 gcctttgagg caaaattcaa acaagatgaa gatgtaaaat ttaaacttaa agcaaaaaga
      841 aataaacttc ttggaaaatg ggctggtgaa actttgcaaa aagaaaatct cgaagagtat
      901 attaaagaag taagagaatc aaatttagta aaaccgggag acgaagatat tatagaaaag
      961 atcatgaaag atttttcctc aaacaataaa gaaataactc gtgaggaact gcttcaaaaa
     1021 tttactgaat gtgaaaaaaa agctagagaa gaattaattg aacaaaactc ataaatattc
     1081 ccagaaatat atattaactt ataaaaactt attgagtggg tagatctact tttaatatat
     1141 aattaggatt ttttgattgc ttaattattg taggaattat ctctttatac gcgtcccaaa
     1201 aaccattttt tggtggtggc atctgttctt tcaacacatc gtgaagtttt tttaaattgt
     1261 atgcaggaac cattggaaac atatggtgct ctagatggta ttccatgttc cagtttagga
     1321 aactaaaaaa aggatttaat ttgacggttc tgacacataa tctgtgatcc tttatattat
     1381 tttttaatcc agcatgttgc ataaaatgta tgcactgcat aaaggtcgtt ccatacataa
     1441 aaggtaaaag caaataaaga gttggaagcc aactttttaa gattagagaa aaaattatta
     1501 tagtcagcca aatagaaaca tgaattcttg caaataatct acaagaaggt cttttttttt
     1561 ctggtataca atcttccata acaggtgtca ttattccgat tgcgtgctta attgtctcca
     1621 aatgaaagaa taaaaaagtt ttatgaataa ttaaaagatt cccaccaggt atgtgcatca
     1681 gtaaaacata aaataaatcc gtcggtttag taatttgtat ttcataatca taaggcccat
     1741 cggttgatag ggtgtggcta tgatgacgaa aatgactcca tctccatcga actggttcat
     1801 aactatacat aaatgaagta atttgataaa aaatttcatt taaccagcga gacttaaagg
     1861 cagatctatg accacagtca tgccatatag gatttgatga catataaata ttaccataaa
     1921 caaagaacca aaacacagcc caccaagttc cccagtttat aaaagccata tatccagaaa
     1981 aaactaaaac aatgatccaa agagagacat gtttaatacc ttgccaatca gaacgtttga
     2041 ttagattttg atatacttgc ttatcaatgt cacatttgta ccatttgttt aaatctacct
     2101 tcatataaaa aacaacgtta attttatagt ccttacaagg aaggttgaat taaaaaaagc
     2161 aaacatagga tttttattaa aagttcaaag attcatataa tcttttaaat gtttattttc
     2221 atctttatat ttctctatta cgtgattgac tacatctcca acagagacga tacccagaat
     2281 tttttttcct tccactatag ggatatgcct aattttattt tgggttatta tttccataag
     2341 ttcagtgaca gaggtattga gattacaagt aattatttct ttggtcatta tattagtgac
     2401 tggtaagtcg tttttaaatt cgtccttaaa aataaatcga gcaaggtcgc gttcagaaac
     2461 tattccgatt atatcgttat tttgtttgct cactaccggc atagctccaa tattgtgttt
     2521 ttgaagtttt tcactagcgg actttagggt gtcatgttca cttaacgtaa aacattttct
     2581 tttagaaagt tttgtaacaa gtcctcctac catagcttta ctcaactaat tttatcattt
     2641 tttgagtttc aacaatcacg ccagcgtttt ctctcatttt aatcatgtct gggtctttta
     2701 gtatttcttg catagtttcc attg
//
LOCUS       DTVV01000483            3116 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2445151, whole genome shotgun sequence.
ACCESSION   DTVV01000483 DTVV01000000
VERSION     DTVV01000483.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2445151"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /gene="pheS"
                     /locus_tag="EYO86_05780"
     CDS             <1..903
                     /gene="pheS"
                     /locus_tag="EYO86_05780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIC42574.1"
                     /translation="KGKELNLFKSFFEQTINNKKSDLEISALNEKLENEQIDATLPPR
                     DFNTGKIHPISQTIYKIIDIFSSMGFSVESGPDIETDFNNFTALNIPSHHPAREMQDT
                     FYIEDKINNEDKVLRTHTSPVQVRTMLETKPPIRIIVPGRTYRSDSDATHTPMFHQVE
                     GLLIDNSSSMAHLKGCLIDFLKEFFEINDLQYRFRPSYFPFTEPSAEMDIAFTKNNDV
                     WKVGAGDQWLEILGCGMVHPKVLENCKIDSKTFQGFAFGMGIERLSMLKYGITDARSF
                     FESDFRWLNHYGFDPLDLTTSSGI"
     gene            905..>3116
                     /locus_tag="EYO86_05785"
     CDS             905..>3116
                     /locus_tag="EYO86_05785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:PRK00629.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIC42575.1"
                     /translation="MKFTLSWLKDHLDTTADIKTIVNTLTNIGLEVEFVEDKSEKFKY
                     FTVAKVVNATKHPNADRLKVCEVQTNKGNFQVVCGASNAQTGMLGIFAPIDTFVPGTK
                     MHLKKSEIRGVESCGMLLSERELGISDEHEVIIELSNVHKIGDPVAKIFGFDDPVIEI
                     NITPNRSDCLSVRGIARDLAAAGLGSLIELKVNKIKGGFVSGVKWLRKFNQKDKYLCP
                     GVAGRFFKNVNNTSSPEWLKSRLTAIGLRPISALVDITNYITHDLGRPLHVYDADKLS
                     GNLTMRKAVAGEKCKTLDEKEYLLSEDMVVISDDKNLHGIGGVIGGLESGCSLETRSV
                     FIEVALFDPISVTKTGRKLNLQSDARYRFERGVDPTSIEWGVDMATQMITQICGGEVS
                     TIEADKSSIQKNKIINFDTNSTKTLGGISIDTKDQTTILNNLGFLVKSKKNTVIEITV
                     PTFRPDIDGPADIVEEILRIYGFDKIIPISLSKDINNNKETLSANLKSFYKSKRLIAN
                     RGYLETVSWSFIDSKEANYINNNVSIDIKNPISTDLSSMRPSAFPNLLSSINPNVARL
                     YTNGKLFEVGPNFNGLEEVDQQMVATGIQYGSSSSSSWLNEARVTDVFDVKSDIYYVL
                     EQLNVPIEGLIFKTLRNNVFHPGKSAQLILGKSIIANFGELNPLLLKRFDIKAVVCGF
                     EIFIDKLEQFQIKKTSTKKAYDNNPYQVVERDFSFLFPKNIKAIDLINNIKKIDK"
BASE COUNT         1135 a          450 c          569 g          962 t
ORIGIN      
        1 aaagggaaag aattaaattt attcaaatca ttttttgaac aaacaattaa caataaaaaa
       61 agtgatttag agatttcagc acttaatgaa aaattagaaa atgaacaaat agacgccact
      121 ctacctccaa gagattttaa tacaggaaaa attcatccaa tcagtcaaac catatataaa
      181 attatagata tattttcatc gatgggtttt tctgtggaga gcggtcctga tattgaaacg
      241 gattttaata attttacagc attgaacatt ccttctcacc atccagctcg agaaatgcaa
      301 gatacattct atattgaaga caaaataaat aatgaagata aggttttaag aacgcacaca
      361 agccctgtgc aagtaagaac tatgcttgaa acaaaacctc ccattagaat tattgttcca
      421 ggaagaacct acagaagtga ttctgatgcc acgcatacgc ccatgttcca tcaggttgag
      481 ggtttattaa tcgataactc atcgtccatg gcacatttaa aaggatgttt aattgatttt
      541 ctaaaagaat tttttgaaat taatgattta caatatcgtt ttcgaccaag ttattttcct
      601 tttacagagc ctagtgcaga aatggatata gcctttacaa aaaacaatga tgtttggaaa
      661 gttggagcag gagatcaatg gcttgaaatt ttaggatgcg gaatggttca tccaaaagtt
      721 ttggaaaact gcaaaataga ttcaaaaacc tttcaaggtt ttgcttttgg aatgggcata
      781 gaacgattat caatgcttaa atatggaata actgatgcaa gaagtttttt tgaaagtgat
      841 tttagatggc ttaatcatta tggttttgat cctttggact taacaacttc ttcaggtata
      901 taaaatgaaa tttacactta gttggttaaa ggatcattta gacacaacag ctgatattaa
      961 aacaattgta aacacactga caaacatagg tttagaagtt gaattcgttg aagataaatc
     1021 agagaaattt aaatatttta ctgtggcaaa ggtagttaat gcaactaaac accctaatgc
     1081 agatagatta aaagtttgtg aggttcaaac aaataaagga aattttcaag tagtttgtgg
     1141 agcctctaat gctcaaacag gaatgctagg tatttttgct ccgatagata cttttgtacc
     1201 aggtacaaaa atgcacttaa agaaatcaga aatacgaggc gttgaatctt gtggtatgct
     1261 tttgtctgag agagaacttg gtatttcaga tgagcatgaa gtcataatag aattaagcaa
     1321 cgtccataaa attggtgacc ccgttgctaa aatttttgga tttgatgatc cagttataga
     1381 aataaatata acgcccaata gatccgactg cttatcagtc agaggaattg cacgtgactt
     1441 agctgcagca ggcctaggtt ctcttataga attaaaagtt aacaaaatta aaggtggctt
     1501 tgttagtggt gtaaaatggt tgagaaaatt taatcaaaaa gataaatatt tatgtcctgg
     1561 agtggcaggt cgttttttta agaatgtgaa taatacatca agtcctgagt ggttaaaaag
     1621 tagactaacc gcaattggtt tgcgaccaat tagtgcgcta gtcgatatca ctaattatat
     1681 aacccatgat ttaggccgcc cacttcatgt ttatgatgca gataagctaa gtggtaactt
     1741 aacaatgcga aaagcagttg ccggggaaaa atgtaaaaca ttagatgaga aagaatatct
     1801 tctctcagaa gacatggttg taatcagtga tgataaaaat ctccatggaa ttggaggtgt
     1861 aataggaggt ctggaaagtg gctgctcttt agaaactcgc agtgttttta tagaggtagc
     1921 tttatttgat cccattagcg taaccaagac aggaagaaag ctaaatttac aaagtgacgc
     1981 acggtaccga tttgaaagag gtgttgatcc aacttcaatt gaatggggtg ttgatatggc
     2041 tactcagatg ataacgcaaa tatgtggagg ggaggttagt acaatagaag cagacaaatc
     2101 ttcaattcaa aaaaataaaa ttatcaactt tgatacaaac tcaacaaaaa ctcttggtgg
     2161 gatcagtatt gataccaaag atcagacaac cattttaaac aatcttggtt ttttggtaaa
     2221 atccaaaaaa aacactgtaa ttgaaataac tgtgccaact tttagaccgg atattgacgg
     2281 acctgctgat atagttgaag aaattttaag aatttatggc tttgataaaa ttatccctat
     2341 ttctttgtca aaagatatta ataacaataa agaaacatta agcgctaatc ttaaatcatt
     2401 ttataaatca aaaagactta ttgctaatcg cggatatcta gaaacagttt cttggtcatt
     2461 tatagattcg aaagaggcaa attatataaa taataatgta tcaatagata ttaagaatcc
     2521 tattagcact gatttgagtt ccatgagacc atccgccttt cccaacctct tgagttctat
     2581 aaatcctaat gtagctaggt tatataccaa tggcaaattg tttgaggttg gaccaaactt
     2641 taatggactt gaagaagttg accagcaaat ggtagcaacg gggatacaat atggttcatc
     2701 tagctcttct tcttggttaa atgaagcaag agtaactgat gtatttgatg taaaaagtga
     2761 tatttattat gttcttgagc aattaaatgt acctatagaa ggcttaattt ttaaaacatt
     2821 gcgtaataat gtatttcatc caggtaaatc agcacaatta atattgggaa aaagtatcat
     2881 agctaatttt ggcgaactaa accccttact tttaaaaaga tttgatatta aagctgttgt
     2941 ttgtggtttt gaaatcttta ttgataagtt agaacaattt caaatcaaaa aaacttctac
     3001 caaaaaagca tatgataata acccctatca agtcgtagaa agggattttt catttttatt
     3061 tcctaagaac attaaggcaa ttgatttgat caataatatt aaaaaaattg ataaga
//
LOCUS       DTVV01000484            1386 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2447765, whole genome shotgun sequence.
ACCESSION   DTVV01000484 DTVV01000000
VERSION     DTVV01000484.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2447765"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(52..606)
                     /locus_tag="EYO86_05790"
     CDS             complement(52..606)
                     /locus_tag="EYO86_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017093964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIC42576.1"
                     /translation="MNLSELPTNLPIPENDGKCEHLLNAIIPPISLPNQDGNLLKLNR
                     ADTFLLVVYCYPMTGHPEKALPSNWDAIPGARGCTPQTCSFRDKYDDLIKLNALPIGV
                     STQSVEDIKEMTTRLRIPYDVLSDQGLLLAEKLNLPTFSVGEKKFIKRLTLIVEKSVI
                     KHVFYPIFPPDKHIIEVLEWLKKK"
     gene            696..>1386
                     /locus_tag="EYO86_05795"
     CDS             696..>1386
                     /locus_tag="EYO86_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIC42577.1"
                     /translation="MVTPRFADVATFFRLPIIKDLNQLDYCLCGVPWDGGTTNRPGAR
                     HGPREIRNASSLIRLYHPISLKSPYDKFNIADIGDCPVNPADLHNSLKKIEKFYLSII
                     ESKTIPLSIGGDHLVSLPILRALGKKEPLGLFQFDSHSDTWDSYFGGYKYTHGTPFRR
                     AIEENLIDPKKYVMIGIRGSLYDPNDMKWARQQGITIITIDEYYEMGFTEAMKIVKNT
                     LGDTQAYLTFDI"
BASE COUNT          456 a          231 c          240 g          459 t
ORIGIN      
        1 aaatggatgt attataaaaa atgaaaataa ttattaaaaa caaacgcatt attatttttt
       61 ttttaaccat tccaatactt ctataatatg tttatcaggt ggaaagatag gataaaaaac
      121 atgtttaatt actgattttt ccacaattaa ggttagtcgt ttaataaatt ttttttctcc
      181 aacagaaaaa gttggtaaat tcaatttttc tgcaagtaat aacccttgat cactcagaac
      241 atcataaggg atgcgtaaac gtgttgtcat ctctttaata tcttctactg attgcgtaga
      301 aactcctatc ggtaaagcgt ttaactttat taaatcatca tatttatcac gaaatgaaca
      361 tgtttgagga gtgcaccctc gagcacctgg aatagcgtcc caattgcttg gtaaagcttt
      421 ttcgggatga ccagtcatcg ggtaacaata aacaactaat agaaaagtat ccgcacgatt
      481 taacttgagc aagttgccat cttgattggg caacgatata ggtggaataa ttgcattcaa
      541 taaatgttca cactttccat cattttctgg tatgggtaaa tttgttggta actcggataa
      601 attcataatg atatattctt tggtatggct aataatgtaa taaataatat aatatttaca
      661 ataattttat gactaaaata caaccaatct ccagtatggt tacccctcgt tttgcagacg
      721 tggcgacttt ttttcgcctt cccattatta aagatttaaa tcaacttgat tattgtttgt
      781 gcggcgttcc ttgggatgga ggcacaacaa acaggccagg cgctagacat ggacctcgcg
      841 aaatacgcaa tgcatcatca ttgataagat tgtatcatcc aatttctcta aaaagtcctt
      901 acgataaatt taatattgct gacattggcg actgccctgt taatccggct gatttacata
      961 actcgctaaa aaaaattgaa aaattttacc ttagcattat tgaatctaag actatccctt
     1021 tgtctattgg tggggaccat ttagtatcct taccaattct gcgtgctctt ggaaaaaaag
     1081 agccgttggg tttatttcaa tttgattcgc attcggatac ttgggattct tattttggag
     1141 gatataaata tacacatggc actccctttc gaagagcaat tgaagaaaat ctgattgatc
     1201 cgaagaagta cgtcatgatt ggtattagag gatcactcta tgatcctaac gatatgaaat
     1261 gggcacgcca acaaggaatt acaattatca ccattgatga gtattatgaa atgggattta
     1321 cagaagccat gaaaattgta aaaaatactt tgggtgatac tcaggcatat ttaacatttg
     1381 atattg
//
LOCUS       DTVV01000485            1506 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2449057, whole genome shotgun sequence.
ACCESSION   DTVV01000485 DTVV01000000
VERSION     DTVV01000485.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2449057"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="EYO86_05800"
     CDS             complement(<1..625)
                     /locus_tag="EYO86_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42578.1"
                     /translation="MNFFGIMNFKEKIFGHKDLVSIGSANLLGNGISAIFWFSLASLI
                     NPEEYGQIHYFLAIAGMAQLLSLISTTNALQVYVAKNIKIHSTLFFISIIAGIVSSLV
                     VFLFFSRTDTSLLVLGYIIFELSNGFLLGKKLFSNYAKFFLTQKILTVIFGFGLYFTF
                     GVDGIIFGLVLSYVPYIWILVNEFRNTRIDFSLLKPRKGFLLNNYGIN"
     gene            701..>1506
                     /locus_tag="EYO86_05805"
     CDS             701..>1506
                     /locus_tag="EYO86_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIC42579.1"
                     /translation="MIKLFEPHVGIEEVKSVENVLRSKLWASGTGTGKVLEFENKFRK
                     YINSKNCIAVNSGTAALNLALTMCDIKNKEVILPSLTFVATANAVIMNGGKPVFVDVD
                     PNTLNIDPEKIENHISKNTKAILPVHFGGLSCDLNKIHSLAKKYNLKIIEDAAHAAGA
                     SYKNKKIGSHSMAVCFSFHPIKNLAMPTGGLISINSKNHEKIRKLLSARRWCGITDRK
                     NTLYDVKEIGNNYYMNELSAVIGLTQLKKLDKLNSIRKKTAKRYYQEIKL"
BASE COUNT          603 a          209 c          227 g          467 t
ORIGIN      
        1 tgtttatgcc ataattatta aggagaaatc ctttacgcgg ttttaacagc gaaaaatcaa
       61 ttctagtatt acgaaattca ttaactaaaa tccaaatata tggaacatag ctcaatacca
      121 aaccaaagat aataccatct acgccaaagg taaagtataa gccaaaacca aaaatcactg
      181 ttaaaatttt ttgagttaag aaaaatttgg cataatttga aaataatttc tttccaagaa
      241 gaaatccatt tgaaagctca aatatgatat agcccagtac aagtaaactt gtatcagttc
      301 tagaaaaaaa tagaaaaact actagggaag atactattcc cgcaattatt gatataaaaa
      361 ataatgttga atgaattttg atattttttg ctacataaac ttgtaaggca tttgtggttg
      421 aaattaacga tagtagttgt gccattcctg caattgctaa aaaataatga atttgaccat
      481 attcctcagg attaattaaa gatgctaaag aaaaccaaaa aatagcagat ataccatttc
      541 caagtaaatt tgcacttcct atagacacaa gatctttatg cccaaatatt ttttctttaa
      601 agttcataat tccaaaaaaa ttcattaaaa ttaaaatctg ttattattaa tttatgcata
      661 ggctcttaaa tttaggttaa aaaagtgtga ttataagaaa atgatcaaat tattcgaacc
      721 acacgttgga attgaggagg taaaatccgt tgagaatgtt ttacgaagta agttatgggc
      781 ttctggtact ggtacgggta aagtattaga atttgaaaat aaattcagaa aatatatcaa
      841 ttcaaaaaat tgtattgcag ttaatagcgg aactgcagca ttaaatctag cacttactat
      901 gtgtgatatt aaaaataaag aagttattct gccatcttta acatttgttg ctactgctaa
      961 tgcagttatt atgaatggcg gaaaaccagt ttttgttgat gttgatccta atacactgaa
     1021 tattgatcca gaaaaaattg aaaatcatat atctaaaaac acaaaagcga ttctgcctgt
     1081 ccattttggc ggcttatcat gtgatcttaa taaaattcat tcattagcaa aaaaatataa
     1141 tctaaaaata attgaagatg ctgcacatgc tgcaggagct agctacaaaa ataaaaaaat
     1201 tggttcgcat agtatggctg tatgttttag ttttcaccct ataaagaatt tagctatgcc
     1261 aactggtggg ctgatttcaa ttaacagtaa aaaccacgaa aaaatccgaa agcttctgtc
     1321 tgcgagacga tggtgtggta ttacagatag aaaaaacaca ttatatgatg ttaaagaaat
     1381 tggaaataat tattacatga acgaattatc tgccgttatt ggtttaacac aattaaaaaa
     1441 attggataaa ctaaattcca ttcgtaaaaa aacagcaaaa agatattatc aagaaataaa
     1501 attaaa
//
LOCUS       DTVV01000486            1611 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2453072, whole genome shotgun sequence.
ACCESSION   DTVV01000486 DTVV01000000
VERSION     DTVV01000486.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2453072"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="EYO86_05810"
     CDS             <1..683
                     /locus_tag="EYO86_05810"
                     /inference="COORDINATES: protein motif:HMM:PF00459.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIC42580.1"
                     /translation="LVTEVDLAVEEKLNNILCSLLPNSLFVGEEKFSLNSKIFDCYKQ
                     NQYCWTVDPIDGTANFVNGKEKFAIMVALTFSDQIIQSWIYKPLTEEICSALLGEGTF
                     INDEKIIITNNRNISEASGSISSKYWNETFDLKMKEIRQKFKHVKNYGCIGFEYIEIA
                     TNIRDFAILSNLSPWDHIPGILIVREAGGFDIHFDKTPYAHNIKKKNLVVSGSITLQN
                     EIINLIKE"
     gene            694..1557
                     /locus_tag="EYO86_05815"
     CDS             694..1557
                     /locus_tag="EYO86_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017971690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC42581.1"
                     /translation="MNKVSFVGLGVMGYPMAGFLKKNNFNVTVYNRTQSKSEKWSKEY
                     GDEYAITPAKAVIDSEAIFICVGRDEDLKEVMDGREGILSNTKEGAIIVDHTTASADI
                     ARGYSRKCQDLNKFFLDAPISGGQVGAENGQLSIMVGGEKEAFEKVKHILSSYGKAIE
                     LIGPSGSGQLTKMINQICIAGLVQGLSEAMAFGEKAKLDMEKVLRVISKGAAQSWQME
                     NRYRTMLDGKFDYGFAVDWMRKDLSYCFNEAKKNNSKLPITKIVDEYYQEVQENGGNR
                     WDTSSLISLLK"
BASE COUNT          599 a          195 c          307 g          510 t
ORIGIN      
        1 acctagtaac tgaagttgat ttagctgttg aagaaaagtt aaataatata ttgtgttctc
       61 ttttaccaaa ctcacttttt gttggagaag aaaaattttc tttaaattct aaaatatttg
      121 attgttacaa gcaaaatcaa tattgttgga cagtagatcc tattgatggc actgctaatt
      181 ttgttaacgg taaagaaaaa tttgcaatca tggtagcttt aactttttcc gatcaaatta
      241 ttcaatcttg gatttacaaa ccgttaactg aggagatctg ctcagcttta cttggagaag
      301 gaacttttat taatgatgaa aagattataa ttactaataa tcggaatata tctgaagcat
      361 caggctcaat tagctctaaa tattggaatg aaacatttga tttaaaaatg aaagaaattc
      421 gtcaaaaatt caaacatgta aaaaattatg gttgtatagg atttgaatat atagaaatag
      481 ctacaaatat aagggatttt gctattcttt caaatttatc accttgggat catataccgg
      541 gtattttgat tgttagagag gcaggtggat ttgatattca ttttgacaaa acaccttacg
      601 cacacaatat taaaaaaaaa aacttagttg tatccggttc aattacattg caaaatgaaa
      661 taatcaattt gataaaggag taaatacatg aatataaata aagtatcttt tgttggtcta
      721 ggtgtcatgg gttatccaat ggcgggattt ttaaaaaaaa ataattttaa tgttacagta
      781 tataacagaa cacaatcaaa gtctgaaaaa tggtctaaag aatatggtga tgagtatgca
      841 ataactcctg ctaaggcagt tattgattct gaagcaatat ttatatgtgt tggtagagat
      901 gaagatttaa aggaagtaat ggatggaagg gaaggtatac tttccaatac caaagaaggg
      961 gctatcattg ttgatcacac aactgcatca gcagatatag cacgcgggta ttctcgaaaa
     1021 tgtcaggatt taaataaatt ttttttagat gccccaattt caggagggca agtaggtgct
     1081 gaaaatggcc aattgtcaat tatggttgga ggagaaaaag aggcttttga aaaagttaag
     1141 cacatactct caagttatgg aaaagcgata gaattaatag ggccatctgg cagcggtcag
     1201 ttaaccaaaa tgattaacca aatatgtata gcaggtttag tccaaggttt atctgaagct
     1261 atggcatttg gggaaaaagc aaaattggat atggaaaaag tattaagggt tatttctaaa
     1321 ggtgctgcac aatcgtggca aatggagaat agataccgta ctatgctaga tggcaaattt
     1381 gattacggat ttgcagttga ttggatgaga aaagatttat cttattgctt taatgaagct
     1441 aaaaaaaaca attctaaact tcctattact aaaattgtag atgaatatta tcaggaagtt
     1501 caagaaaatg gcggtaatag atgggataca tcaagtttaa tttcattact aaaataaatt
     1561 caattagttt atatttaatt agatggacac taaacttgaa gataagtaca c
//
LOCUS       DTVV01000487            1147 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2454274, whole genome shotgun sequence.
ACCESSION   DTVV01000487 DTVV01000000
VERSION     DTVV01000487.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2454274"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="EYO86_05820"
     CDS             complement(<1..1046)
                     /locus_tag="EYO86_05820"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIC42582.1"
                     /translation="MHYLSTRNDKLRETFTNILFQGLSREGGLFLPVEWPNIDINTLR
                     DKSYEEVALHIMRPFVGGDLKDDNLYEIISSSYDDFRHPKIAPLVNVDSNKYILELFY
                     GPTFSFKDYALQFLGNLFEHLVRTSKKQITVLGATSGDTGSAAIEAFKGKKDIKVFIL
                     HPYNKVSDIQRRQMTTVTDSNIFNIAVEGTFDDCQKTVKDLFVDQEIQKKTSLTAINS
                     INWSRIMAQAVYYFWSYLQTEKDKISFIVPSGNFGNIFSARVAKHMGLPIDRLHISTN
                     QNDILHRIISQGQMTMNKVEHTFSPSMDIQVASNFERQLFEIFNNNSNSIVDVMREFQ
                     NNKSYTLSNSIISVL"
BASE COUNT          372 a          210 c          194 g          371 t
ORIGIN      
        1 aggacagata tgatagaatt gcttaaggta taggacttat tattttgaaa ttctctcata
       61 acatctacaa tggaattgct gttattatta aaaatttcaa atagttgacg ctcaaaatta
      121 ctagcaacct ggatgtccat actgggactg aaagtatgtt ccactttgtt cattgtcatt
      181 tggccttgag aaataattcg gtgcaaaata tcattttgat tggttgaaat atgtaaccta
      241 tcaataggaa gccccatatg tttggccaca cgagcagaaa aaatattgcc aaaatttccg
      301 gaaggaacaa taaagcttat tttatctttt tctgtttgaa gataggacca aaaataatag
      361 accgcttgag ccataatgcg agaccagttg atggaattga tagcagttag agatgttttt
      421 ttttggatct cttgatctac aaataaatct ttcactgttt tttgacaatc atcaaaagtg
      481 ccttctacag caatattaaa aatgttcgaa tcagtcacag tcgtcatttg gcgcctctgg
      541 atatccgaaa ccttattgta agggtgcaaa ataaaaactt taatatcctt ttttccttta
      601 aaagcttcta ttgcagccga cccagtgtca ccggatgtcg cccctaacac cgttatttgt
      661 tttttagagg tacgtacaag gtgctcaaac aaatttccca aaaactggag ggcataatcc
      721 ttaaaagaaa aagttgggcc ataaaataac tcaaggatat acttgtttga atcaacatta
      781 acgagcggtg caattttggg atggcgaaaa tcatcgtacg atgaactaat tatttcatac
      841 aaattatcat cttttaaatc cccccctaca aaagggcgca taatatgtaa tgccacttct
      901 tcataagact tatcacgtaa agtattaata tctatatttg gccattctac aggaagaaat
      961 aaccctcctt cccttgaaag tccttgaaaa agaatattgg taaacgtctc ccgtaatttg
     1021 tcatttcttg tgcttaaata atgcatcagt atcctatcct aattggcata atttaagtca
     1081 tcttgtagtt gcaattggga atatagttgg catttatcca ataatgcctc atgcgttcct
     1141 gagtcca
//
LOCUS       DTVV01000488            1058 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2455330, whole genome shotgun sequence.
ACCESSION   DTVV01000488 DTVV01000000
VERSION     DTVV01000488.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2455330"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(95..>1058)
                     /locus_tag="EYO86_05825"
     CDS             complement(95..>1058)
                     /locus_tag="EYO86_05825"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01408.20,HMM:PF02894.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIC42583.1"
                     /translation="RCYADYLSMATEESKKPDGIQALGIMTPSGDHYRIAKPFINNNV
                     HIICDKPLTATVEDALKLEKLVKKKKVIFALTHNYSAYPMLREAKSLVDRGKIGKVLL
                     ANVEYPQGYTVGVKKKDEASTLKWRLDKKLCGPSMILAEIGTHSYHLLHYVTGLEVSE
                     VSAEVNSLSPELSVDDNAFLILRMQNKARASIWLSSTATGGENGLKIRVYGTKGAVEW
                     FQDDPNHLKFTELNKSTKIITRANNEVSNFSLESSRLAAGHPEGFFEAFANIYTEFAD
                     TISGYRKQKNKKFNFPTVEDGVKGMKFIFAAKKSSNNNARWIKL"
BASE COUNT          312 a          216 c          176 g          354 t
ORIGIN      
        1 aatctagtta acaatcaaga tgtattataa ggttattctc agcataacca agtgttataa
       61 cagcgaatat ataaaaatca aggcagaaaa aaatttacaa ttttatccag cgagcattat
      121 tgttggaaga tttttttgca gcaaatataa atttcattcc cttgacaccg tcttctacag
      181 ttggaaagtt aaattttttg tttttttgtt ttctataacc actaattgta tctgcaaact
      241 cagtgtaaat gtttgcaaaa gcctcaaaaa aaccttcggg atgacctgcc gccaaacgtg
      301 acgattccaa agagaaattt gatacctcat tattggcccg tgttataatt tttgttgatt
      361 tgttgagttc cgtaaacttt aaatgattgg gatcatcctg aaaccattca accgctccct
      421 tggttccata aaccctaatt ttaaggccgt tttcaccacc cgttgcagtg gaggataacc
      481 agattgaagc tctggcctta ttctgcattc ttaaaatcag aaatgcattg tcatcaacag
      541 acaactctgg agaaagtgag tttacttcgg cactaacctc gctaacttcc aatcccgtta
      601 cataatggag cagatggtat gagtgcgttc caatctcggc caaaatcatg gatggtccgc
      661 ataatttttt gtccaatcgc cattttaatg tagaagcctc gtctttcttc ttcactccga
      721 ccgtataacc ctggggatat tcgacattag ccaataaaac ttttccaatc tttcccctgt
      781 caactaaact tttagcttcc ctcaacattg gataggcaga ataattgtga gttaatgcga
      841 atataacttt ttttttcttc acaagttttt ctaattttaa tgcatcctca acagtagccg
      901 ttagaggttt gtcacaaata atatgtacat tgttattaat gaaaggttta gcaattctat
      961 agtgatcgcc cgaaggggtc attattccca gggcttgaat tccatcgggc tttttactct
     1021 cttctgttgc cattgataaa taatctgcgt aacaacgt
//
LOCUS       DTVV01000489            1160 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_2455650, whole genome shotgun sequence.
ACCESSION   DTVV01000489 DTVV01000000
VERSION     DTVV01000489.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2455650"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(77..670)
                     /locus_tag="EYO86_05830"
     CDS             complement(77..670)
                     /locus_tag="EYO86_05830"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001326580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIC42584.1"
                     /translation="MKKNLNNLATFILTSCKYVTNHEIINNELIICTKISNIYNLLDD
                     LKKIDDLKFEMLLDITAVDYPQRSKRFELVYILLSLKNHLRIKVKIFLNDNEIVPSIS
                     NLYKSAGWYEREVWDMYGISFNGNNDLRRILTDYGFEGYPLRKDFPLTGFVELRYDEA
                     KKKVEYSKVKLTQDYRNFDFLSPWEGGTLPGDEKADN"
     gene            complement(676..1152)
                     /locus_tag="EYO86_05835"
     CDS             complement(676..1152)
                     /locus_tag="EYO86_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIC42585.1"
                     /translation="MSKEQYKTKGFITTKFNNLINWARSNSMWPMTFGLACCAVEMMH
                     TYMSRYDLDRFGIVTRGSPRQSDVMIVAGTLCNKMAPALRKVYDQMPDPKWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIMQLQKKIKRENVFYR"
BASE COUNT          396 a          202 c          139 g          423 t
ORIGIN      
        1 catgcgcagc aggatgttgt gggccaaaat taatagtgag gggtttgatt ttaacttctt
       61 tcattggaaa ttaaaattaa ttatctgctt tttcatctcc tggtaaagtg ccaccttccc
      121 aaggacttaa aaaatcaaaa tttctataat cttgtgttaa ttttactttt gaatattcaa
      181 cttttttctt cgcttcatca tatcttaatt ctacaaatcc tgttaaagga aagtcttttc
      241 ttaacggata accttcaaaa ccataatcag ttaaaattct tcttaagtca ttattaccgt
      301 taaatgatat accgtacata tcccaaactt ctcgctcgta ccaacctgca gatttataaa
      361 gatttgaaat agatggaaca atttcattat cattcaaaaa aattttaact tttattctta
      421 aatgattttt taaacttaat aaaatataaa ctaattcaaa tctttttgat ctttgtggat
      481 aatcaacagc agtaatatcc aataacatct caaactttaa atcatctatt ttctttaaat
      541 catcaagtaa attgtaaata ttagatattt tagtacatat tattaattcg ttattaataa
      601 tttcatgatt agttacatat ttgcatgagg tcaaaataaa agtagcaaga ttatttaaat
      661 tttttttcat aaattctatc tataaaaaac attctctctt ttaatttttt tttgaagttg
      721 cataatccca taaagtaaag cttctgcagt aggcggacaa ccaggaacat aaatgtcaac
      781 tggtactatt ctatcacagc ctctaacaac agaatatgaa taatggtaat aaccaccgcc
      841 attagcacat gaacccattg aaattaccca ttttggatca ggcatttgat cgtacacttt
      901 cctaagagct ggagccattt tattacataa agtacctgcg actatcataa catctgattg
      961 tcgagggcta cctctagtaa caataccgaa tctatctaaa tcatatctac tcatataagt
     1021 atgcatcatt tctactgcgc aacatgctaa gccaaatgtc attggccaca tagaattaga
     1081 tcttgcccaa tttattagat tattaaactt tgtggtgata aatccttttg ttttatattg
     1141 ttctttagac acatcgttaa
//
LOCUS       DTVV01000490            1572 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_270148, whole genome shotgun sequence.
ACCESSION   DTVV01000490 DTVV01000000
VERSION     DTVV01000490.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_270148"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /gene="murE"
                     /locus_tag="EYO86_05840"
     CDS             <1..1551
                     /gene="murE"
                     /locus_tag="EYO86_05840"
                     /inference="COORDINATES: protein
                     motif:HMM:PF08245.10,HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HIC42586.1"
                     /translation="SHDHLDYHKNMQSYFNSKLYLFRRLLKKNSKIITDEDNQEFKII
                     KKIANKREIKKITIGSNSGNIKILNNKYKQNKQHIKISINSKIFKLEVPLIGYFQIKN
                     LFMAVLAALTCGLNQNRIFTQIHKIKAVPGRLECVANLNNNSNIIVDFAHTPDALEQS
                     LVALEKQFKKKIVIVFGCGGERDKKKRYAMGKIAAKYCRKIYVTDDNPRNENPKKIRN
                     SIIKGCKKLAIDIGSRKKAIKAAIKELKSNEILLVAGKGHEETQNYGNKIINFSDKKL
                     IKEIIQKRKFSFKKTHYHNFLLNKVFNKDKLKNINYNGVSINTKTIKKNNLFFAICGK
                     KTDGHKFVKEAVRKGAIKSIVSKKIKKLSSNKIIKVKNTFSSLNNLAKVTRDNSSAQI
                     VGITGSVGKTTLKNLLSFSLKNYGKVYSSPHSYNNKFGVPLSLSNLKSNIDYGVFEIG
                     MDKKGEIDNLSKIVKPEIAIITNISGAHFKNFNTLKDIAKAVKPRACSGAVGPGSSRL
                     RVINLLRF"
BASE COUNT          675 a          192 c          232 g          473 t
ORIGIN      
        1 agtcatgatc atcttgatta ccacaaaaac atgcaatcct attttaattc aaaattgtac
       61 ctatttaggc gtttactcaa aaagaattct aaaattatta ctgatgagga caatcaagaa
      121 ttcaagatta taaaaaaaat tgcaaacaaa agagaaataa aaaaaattac aattggttca
      181 aactcaggaa atataaaaat tttaaacaac aaatataaac aaaacaaaca acatataaaa
      241 atttctataa attcaaaaat ttttaagttg gaagttccac ttataggata ttttcagata
      301 aaaaatttgt tcatggcagt tttggcggca ttaacttgtg gattgaatca aaatagaatt
      361 tttactcaaa tacataaaat taaagcagtg cctggaagac ttgaatgtgt agcaaactta
      421 aataataatt ctaatattat tgtagatttt gctcatactc ctgatgcatt ggaacaaagc
      481 ttagttgcat tggaaaaaca gtttaaaaaa aaaatcgtaa tagtttttgg ttgcggaggg
      541 gaaagagata aaaaaaaaag atatgctatg ggcaaaattg ctgccaagta ttgtcgaaaa
      601 atttatgtta cagatgataa tccaagaaat gaaaatccca aaaaaataag aaattcaatt
      661 ataaaaggat gtaaaaaatt agctattgat atcggaagca gaaagaaagc aataaaagct
      721 gcaattaaag aactaaaatc aaacgaaata ctcttagttg caggaaaggg tcatgaagaa
      781 actcaaaatt atggtaataa aattataaat ttttcagata aaaaattaat aaaagaaata
      841 attcaaaaaa gaaaattttc ttttaaaaaa actcattatc acaatttttt gttaaacaaa
      901 gtatttaata aggataaact taaaaatata aattataatg gagtgtctat taatacaaaa
      961 actataaaaa aaaataatct attttttgct atttgcggaa aaaaaactga tggacataag
     1021 tttgtaaaag aagccgtcag gaaaggagct ataaaatcca tagtaagtaa aaaaataaaa
     1081 aaactttcta gcaataaaat tattaaagta aaaaatactt tttcttcttt gaataatttg
     1141 gctaaagtta ctagagataa ctcatccgcg caaattgttg gaataaccgg gagtgttggt
     1201 aaaactactt taaaaaatct gctaagtttt tcacttaaaa attatgggaa agtttatagc
     1261 tctccgcatt cttataataa taaatttggg gtacctctta gtctttctaa tttaaaaagt
     1321 aatattgatt atggagtatt tgaaataggc atggataaaa aaggggaaat agataattta
     1381 tcaaaaatag ttaaaccaga aatagctatt attactaata tttctggagc acattttaaa
     1441 aattttaata ccttaaaaga tatagcaaaa gccgtaaaac cccgtgcgtg ctcgggtgct
     1501 gtgggcccag gttcatcaag acttcgcgtg ataaacctcc ttcggttttg atttcctcga
     1561 ctacggttac at
//
LOCUS       DTVV01000491            2875 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_331255, whole genome shotgun sequence.
ACCESSION   DTVV01000491 DTVV01000000
VERSION     DTVV01000491.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_331255"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            25..1566
                     /locus_tag="EYO86_05845"
     CDS             25..1566
                     /locus_tag="EYO86_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIC42587.1"
                     /translation="MQIGVDVGGTNTDGVIMSDGKVLVSDKKVTTANVGDGVFNVIKS
                     VLEKGKISSDKISSVMVGTTHFTNALVERKKLQKIAAIRLSLPAADSLEQMIGWPKDL
                     ISTLSPKKYFAKGGYEVDGREISPLDEDEINKIIDDIGNSDINSIAVNSVYSPLNSAM
                     EEKTAELISKKLPNIKISLSHAIGKVGLIERENATIINAALAGLAEEIIEAFESALQN
                     LNINAPLYISQNDGTLMLPNKVKSYPVLTFACGPTNSMRGAAFLSGLDESVIVDIGGT
                     TTDIGYIKQGFPRQSAVATDVGGVRTNFRMPDIISIGLGGGSIVRENGNKIGPDSVGY
                     LLTEKAKVFGGDTLTATDIAVAKYNLDIGDPSLVNNIDKSLINNSKKQFTKLLEDSID
                     KMKTSSEEVPAIFVGGGTIINKEKVKGISELIIPDNFAVANAVGAALGQVSGEVDRVF
                     HYDKLGRDKTIENAKLEAKEKAKEAGAIEKTIEVMEINENPLAYLPGRSVRLQIKAIG
                     DLENI"
     gene            1572..2666
                     /locus_tag="EYO86_05850"
     CDS             1572..2666
                     /locus_tag="EYO86_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HIC42588.1"
                     /translation="MDKLTLDNLEDFLRGTAFLGTGGGGDPYVGGLMLRQELEKGTDC
                     KIIKGSEVDDNDLIIPIAVMGAPTVIVERLPNAKSCIKALRKMEELMGKKAAALIGAE
                     AGGINGTLPFILSSYTGLPVIDGDGMGRAFPELQMCTFGVYGVNCSPVIVRDEKDNEI
                     IVNAENNHAAEMFARVICMQMGTKSEIALYPMTGKQMKDTAVHHTCTLAYEIGRSIRE
                     ARDTGKSPLNGIIDYFKTSFDKRYCKLLFEGKVTDLLRETTDGWAKGTVKLTSLTDSS
                     KEMTVQLQNEFLIALVDNKPVAMVPDLICLVDLETAEPITAEAVKYGQRVHVIGVSVP
                     ANMRTKEALDTFGPVPFKVYDKYIPIEEIS"
     gene            2748..>2875
                     /locus_tag="EYO86_05855"
     CDS             2748..>2875
                     /locus_tag="EYO86_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HupH"
                     /protein_id="HIC42589.1"
                     /translation="MNINVRVVTPITTKGFRRTEDLNAIGANDFTVSHSEIDIGPGS"
BASE COUNT         1053 a          367 c          569 g          886 t
ORIGIN      
        1 aataacttag tagtttttta aaaaatgcaa ataggtgttg atgtaggagg aaccaatact
       61 gacggcgtaa ttatgtcaga tggcaaagta ttggtatctg ataaaaaagt caccactgca
      121 aatgtaggag acggtgtttt taatgtaatt aaatctgttt tagaaaaagg aaaaataagt
      181 tctgataaaa tttcaagtgt tatggttgga acaacacatt tcactaatgc tttagttgaa
      241 agaaaaaaat tacaaaaaat tgcagctata agattatcat taccagctgc ggatagctta
      301 gaacaaatga ttggctggcc taaagattta atttccactt taagtccaaa aaaatatttt
      361 gctaaaggtg ggtacgaagt cgatggcaga gaaatttctc ctttggatga agatgaaatt
      421 aataaaataa ttgatgatat tggaaatagt gacattaata gtatcgcggt caactctgtt
      481 tattctcctt taaattcagc aatggaagaa aaaacagctg aacttatttc aaaaaaatta
      541 ccaaatatca aaatttccct ctctcatgct attggtaaag taggcttaat agagagagaa
      601 aatgctacta ttattaatgc tgctttagct ggattggctg aagaaataat tgaagctttt
      661 gaatctgcac ttcaaaattt aaatattaat gctcctcttt atataagtca aaatgatgga
      721 actttgatgc taccaaataa agttaagtca taccctgttc tgacttttgc atgcggtcca
      781 acaaatagta tgaggggggc agcattttta tctggtttgg atgaatcagt aattgttgat
      841 ataggtggaa ccacaacaga tattggttat attaaacaag gatttccaag gcaatcagca
      901 gtagcaacag atgttggcgg tgttagaaca aattttagaa tgcctgacat tatatctata
      961 ggtcttggtg gaggaagtat agttagagaa aatggaaata aaattggacc cgatagtgtt
     1021 ggatatttat taactgaaaa agcaaaggtt tttggcggag atactttaac agcaactgat
     1081 attgctgtgg caaaatataa tttagacatt ggagaccctt cattagtaaa taatattgat
     1141 aagtctttaa ttaataattc aaaaaaacaa tttaccaaat tacttgaaga ttcaattgat
     1201 aaaatgaaaa caagttcaga agaagttccc gcgatattcg ttggaggagg aacaattatt
     1261 aataaagaaa aagtaaaagg tatttcagaa ttaattatac ctgataattt tgctgtagct
     1321 aatgctgtcg gtgctgcttt aggtcaagtc agcggagaag ttgatcgagt ttttcattat
     1381 gataaattag gaagagataa aactatagaa aatgcaaaac ttgaagctaa agaaaaagca
     1441 aaagaggctg gggcaattga aaaaactatt gaagtaatgg agattaatga aaacccccta
     1501 gcttatcttc caggtagatc tgtaagatta caaataaaag ctattggtga tttggagaat
     1561 atttaaataa catggataaa ttaacactag acaatcttga agatttttta agaggtacag
     1621 ccttccttgg aactggaggt ggtggagatc cgtacgttgg cggcttgatg ctaagacaag
     1681 aattagaaaa aggtactgat tgtaaaataa tcaaaggctc tgaagtagat gataatgatc
     1741 ttataattcc aattgcagtt atgggggctc ccacagttat tgttgaaaga ctgcctaatg
     1801 caaaatcttg tataaaagct ctaagaaaaa tggaagaatt aatgggcaaa aaagccgcag
     1861 ctcttatagg tgctgaagca ggtggaatca acggtacttt gccattcata cttagctcat
     1921 acaccggttt accagtaatt gatggtgatg gcatggggag agcttttcct gaacttcaaa
     1981 tgtgtacctt tggagtttat ggtgtcaatt gctcaccggt aatagttaga gatgaaaaag
     2041 ataatgaaat aatagttaat gcagaaaata atcatgcagc tgaaatgttt gctcgagtta
     2101 tatgcatgca aatgggtact aaatcagaaa tagctttata ccctatgact ggaaaacaaa
     2161 tgaaggatac cgctgtgcat catacttgta cattggctta tgaaatcggc agatctatta
     2221 gggaagctag agatacagga aaaagtcctt taaatggaat tattgattat ttcaaaacaa
     2281 gttttgataa acgatattgt aaattattat ttgaaggtaa agttacagat ttattaagag
     2341 aaactactga tggttgggct aaaggaactg taaaattaac ttcattaact gattctagta
     2401 aagaaatgac tgtccaatta caaaatgaat ttttaattgc attagttgat aataaacctg
     2461 ttgctatggt tcctgattta atttgtcttg ttgatttaga aactgcagag ccaataacag
     2521 ctgaagctgt aaaatatgga caaagggttc atgtaatagg tgttagtgtt cctgctaata
     2581 tgcgaacaaa ggaagcatta gatacttttg gcccagttcc ttttaaagtt tatgataaat
     2641 atatacctat tgaagaaata agttagcttt gataatttat aactttacaa tttgtatata
     2701 aatatattta atttcaataa gttaaataaa taaaggagaa ttgataaatg aatataaatg
     2761 ttagagttgt gacacctatt actactaaag gctttagaag aactgaagat ttaaatgcta
     2821 taggtgccaa tgattttact gttagtcatt cagagataga tattggccca ggttc
//
LOCUS       DTVV01000492            1907 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_340080, whole genome shotgun sequence.
ACCESSION   DTVV01000492 DTVV01000000
VERSION     DTVV01000492.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_340080"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(75..812)
                     /locus_tag="EYO86_05860"
     CDS             complement(75..812)
                     /locus_tag="EYO86_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC42590.1"
                     /translation="MNNINLKNKVAVVTGGAQGFGLAIAKRFLVSGAQVVIWDIDEKI
                     LLSVEEKNITKIIVDVTNFQKIEEATKETLSLLGRIDILVNNAGIAGPSYTTWEYPIE
                     EWQKIIDIDLSGVFYCCKSIVPVMKKNNYGRIINIASIAGKEGNPNAMPYSAAKAGVI
                     ALTKSLGKELANNNIAVNCITPAAAKTRIFDQISQEHIDYMLSKIPRNRFVKVDELAS
                     MVAWMASEENSFTTGAVFDLSGGRATY"
     gene            complement(805..1893)
                     /locus_tag="EYO86_05865"
     CDS             complement(805..1893)
                     /locus_tag="EYO86_05865"
                     /inference="COORDINATES: protein motif:HMM:PF13899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42591.1"
                     /translation="MLQHIEHPSSIRMSFMVTSFGIVCSFGLLAFFFVLLRYLGVSVG
                     WGMQFQQPIFLMIIAFVLAFFSFNLFGLFEISIPRFMSAGIINQIPKNNYTRDFFNGF
                     FATLMATPCSAPFVGTALTFAFTQSSMIMIVIFVAMGVGMALPYLFVSLFPQLIKFFP
                     QPGPWMKYLKYFLGCLLVATLVWIGNVLLNHFNYYFITVTIILLIGYLSLIYFFKYKT
                     FIFIITLVIFFSLPNFAVFKSVNLKVDSGWLDLTTINLKQLISQNDIVFVDITADWCA
                     TCQFNKINVLQKKSIQELFSKNNVVKVRGDWTKTSSAIESFLQQYQRFGIPFNIVYGK
                     NYPREIILSELLSEKKIIEALNTLLLNE"
BASE COUNT          704 a          347 c          283 g          573 t
ORIGIN      
        1 attgtctgtg gaagaatccg ctagaaaaaa ctattcacga agagagggcg tttttgattt
       61 aggttaaaat aagtttaata cgtagctcga ccaccactaa ggtcaaatac tgcccctgta
      121 gtaaaagaat tttcttccga ggccatccaa gctaccattg aagccagctc atccactttc
      181 acaaaacgat tacggggaat tttggagagc atataatcaa tatgttcttg agatatttga
      241 tcaaaaattc gtgtctttgc agcagcaggc gttatacaat ttacagcaat attattatta
      301 gctaactctt ttcctaaaga ttttgttaag gcaattaccc ctgcttttgc cgcactataa
      361 ggcattgcat tgggattacc ttcttttcca gcaatagaag caatattgat aattctacca
      421 taattatttt ttttcatcac aggcacaatg gatttacaac aataaaaaac accacttaaa
      481 tcaatatcaa taattttttg ccattcttca attggatatt cccatgttgt ataactaggg
      541 cccgcgatac ctgcattatt aaccaaaata tcaatccttc caagtagcga taaagtctct
      601 ttcgtggctt cttcaatttt ttgaaaattt gtaacatcaa caataatttt agtaatattt
      661 ttttcttcaa cagacaaaag aattttctca tctatatccc aaataactac ttgggctcct
      721 gataccaaaa aacgctttgc aatagctagg ccaaaacctt gtgccccccc agtcacaaca
      781 gcaactttgt ttttaagatt tatattattc atttaatagt aatgtattga gtgcttctat
      841 aatttttttt tcagaaagta actccgataa tataatttct cgcggataat ttttgccata
      901 cacaatatta aaaggaatcc caaatcgctg atattgctga agaaaacttt ctatagcact
      961 gctggtcttt gtccaatctc ctcttacctt aacgacatta tttttagaaa aaagttcttg
     1021 aatagatttt ttttgaagaa cattaatttt attaaattga caagttgcgc accaatccgc
     1081 agttatatca acaaaaacaa tatcattttg actgattaac tgctttaagt ttattgtcgt
     1141 gagatcaagc catccggaat caacctttaa attaacagat ttaaatactg caaaattagg
     1201 caaactaaaa aatataacca aagtaataat gaaaataaaa gttttgtatt taaaaaagta
     1261 aataagcgac aagtatccaa tcaataaaat aatagtaact gttataaaat aataattaaa
     1321 gtgattgagc aaaacattac cgatccatac cagggtagct actaacaaac atcccaaaaa
     1381 atatttcagg tatttcatcc atggtcccgg ctgaggaaaa aattttatta attgaggaaa
     1441 taaggaaacg aacaaatagg gcaatgccat gccaactccc atagcgacaa aaattacaat
     1501 cataatcatt gaagattgtg tgaaggcaaa ggtgagggca gtacctacaa aaggagccga
     1561 gcatggtgtc gccattaaag tagcaaaaaa accattaaaa aaatctcgtg tataattatt
     1621 tttcggtatt tgattaatga tgcctgcact cataaaacgc gggatcgata tctcaaataa
     1681 cccaaataaa ttaaaagaaa agaatgctaa aacaaatgca ataatcatta aaaatatagg
     1741 ctgctgaaat tgcattcccc aacctacgct cactcctaaa tagcgcagta gaacaaagaa
     1801 aaaagctaat aaaccaaagg agcaaacaat accaaaggat gtaaccataa aactcatgcg
     1861 gatagaggat gggtgctcaa tatgttgaag catcgataat attttta
//
LOCUS       DTVV01000493            1750 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_399776, whole genome shotgun sequence.
ACCESSION   DTVV01000493 DTVV01000000
VERSION     DTVV01000493.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_399776"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            2..487
                     /locus_tag="EYO86_05870"
     CDS             2..487
                     /locus_tag="EYO86_05870"
                     /inference="COORDINATES: protein motif:HMM:PF00208.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42592.1"
                     /translation="MENVVSLDNADSIKAKIICEAANGPITYRADLRLREKGKVIIPD
                     IYANAGGVTVSYFEWIRNISHIRMGRLNKRYEEHRGEAIFKAIEQISPNKLPKDMINQ
                     LVYGANEEDIISSGLEDTMRVAFQEILEMREKYNLNNYRMATYAIALKKIEKSYLELG
                     I"
     gene            683..1297
                     /locus_tag="EYO86_05875"
     CDS             683..1297
                     /locus_tag="EYO86_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIC42593.1"
                     /translation="MLPINFLLFIQIILFLFITPGSPRVLVVSYAMTYGMKKTVWTAL
                     GDISANAMQMIVVTFVIGSLLIRFPQIMVVMKWLGVAYLLYLAYELYRARMKNFNSSE
                     GDASKNSLSFFRDGFMVAGLSPKALIFFGAVFPNFMDYSGNYIGQFIILAITYVVLDF
                     TTLIIYGVGAKKISLWLQANPKTINLVSSAALLIIALITAFVKV"
BASE COUNT          598 a          286 c          280 g          586 t
ORIGIN      
        1 actggagaat gtagtgtctc tggataatgc tgatagtatt aaagcgaaaa taatctgtga
       61 agctgctaat ggtcctatta cctaccgtgc ggatctacga ttacgtgaaa agggcaaggt
      121 tatcattcct gatatttatg caaacgcagg cggagtcacc gtttcttact ttgaatggat
      181 tagaaatatt agccacatac gtatgggtcg tttaaacaag cgatacgaag agcacagagg
      241 ggaggcaatt ttcaaggcta tagagcaaat ttcacccaat aaacttccca aagatatgat
      301 taatcaatta gtctacggag ccaatgaaga ggatatcatc tcttccggcc tagaggacac
      361 gatgcgagta gcctttcaag aaatattaga gatgagggaa aaatataacc ttaataatta
      421 tagaatggcc acttatgcca tcgctcttaa aaaaatagaa aaatcatatt tagaattggg
      481 tatctaattt ttttttgaca atattttcaa ttttttaaaa ataataattt tcaattattt
      541 tctttaattc catatgctca tgggaccaag aggctgcaaa tgataaaaaa taaattttta
      601 ttaatctatt aataaactta atgaatgaaa taatttagaa ttaggataaa caaatagatt
      661 aacctgagaa actgaaacaa taatgttacc aataaatttt cttctattca tccaaattat
      721 tttattctta ttcattactc caggctctcc aagagtgttg gttgtatcct atgccatgac
      781 ttacggaatg aaaaaaaccg tatggacagc tctgggcgat atcagcgcca atgcaatgca
      841 aatgattgtt gtcacctttg tcattgggtc tcttcttatt aggtttccgc agattatggt
      901 tgtcatgaaa tggttaggcg tggcctatct tttatattta gcgtatgaat tgtaccgtgc
      961 aaggatgaaa aactttaact cctcagaagg agatgcatca aaaaattctc tttccttttt
     1021 ccgtgacgga ttcatggtag cggggcttag ccctaaagcc ctaatatttt ttggagccgt
     1081 ttttcccaac tttatggatt atagtggcaa ttacattggt caatttatta ttctagcaat
     1141 cacctatgta gttttagatt ttacaacctt aatcatatat ggggtgggag caaaaaaaat
     1201 atccttgtgg ctacaagcaa atcctaaaac aattaacctt gtctcatctg ccgccttatt
     1261 gataatagcg ttaattacag ctttcgtaaa agtttaaatt ctttcaataa aaatattttt
     1321 taattaaatg aaagccatgc aaaaaatata gagaagccaa ttaaaataac caacgctaaa
     1381 attaaaaaaa ttattatttc tttcatggga aatttcataa atcaaacgat agtcaattat
     1441 aaaaaaaatt aaagaaaaaa tatttgattc taacctttat taccctctgt taattgctta
     1501 acagctaaaa attattttta aatgaggtat tctttgtatt tttgtttaaa attttctaaa
     1561 tcatctctat tgcgaaaatc tattctataa ctagctaaaa ggtcatcttc tttggaatca
     1621 ccgtatcttc tatgctcaag atcctgaaaa tctcgtttat caatcttatc aatattcgat
     1681 accaataagg ccaattttct ctgtaattca aagctttgtg ggctcctttt gtcaaaagaa
     1741 agtgatttca
//
LOCUS       DTVV01000494            1574 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_4014, whole genome shotgun sequence.
ACCESSION   DTVV01000494 DTVV01000000
VERSION     DTVV01000494.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_4014"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="EYO86_05880"
     CDS             complement(<1..461)
                     /locus_tag="EYO86_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIC42594.1"
                     /translation="MVISSNLGYPRIGPNRELKWSLESFWKKEINETKLLEDISRIKK
                     ENWIIQKKSGIQHVPSNDFSLYDHVLDTCLVVNAIPDRYKRLKNKKNFLDLYFAMARG
                     FQSGSIDIKAMEMTKWFDTNYHYIVPEFKNNQKFKLASTKIIDEFLEAKSFG"
     gene            complement(595..>1574)
                     /gene="mgtE"
                     /locus_tag="EYO86_05885"
     CDS             complement(595..>1574)
                     /gene="mgtE"
                     /locus_tag="EYO86_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIC42595.1"
                     /translation="VKKGLKFPEVTAGRIMATDIVSVSASWTIGKTLKYLRQKKEILP
                     ENIYEVYILDSKRRPIGTLNLNTIVRSTSATVVKNIMNTQFKTIPIDTDQEEIALGFK
                     QSNLIAAPVVDKLGKIVGQITSEDVIDIIEEETEEDLLRLSGVQSDDTYSAVLKTIQS
                     RFGWLLINLFTAIAASFVIGIFESSLRQLVALAILMPIVASMGGNAGTQTLTVAVRAL
                     ALREVTRANALRVIGKEFAVGTINGIIFAVIIGSVAAYWFQMPLLGIIIGAAMIINLM
                     VACLSGILIPLILDKTGIDPAIASTVVLTTITDIFGFFVFLGLGSIYLL"
BASE COUNT          523 a          281 c          210 g          560 t
ORIGIN      
        1 ccaaaacttt ttgcttctaa aaattcatca attatttttg ttgaagccag tttaaatttt
       61 tgattatttt taaactccgg aacaatataa tgatagttag tgtcaaacca tttagtcatt
      121 tccattgctt taatatcaat gctcccgctt tgaaaacctc ttgccattgc aaaataaaga
      181 tccaaaaaat tttttttgtt ttttaatctt ttatatctat ctggtattgc attaaccact
      241 aagcaggtat caagaacgtg atcatataaa gaaaaatcat tagagggaac atgttgaata
      301 ccggattttt tttgtatgat ccaattttct ttttttattc ttgatatatc ttctaataac
      361 tttgtttcgt taatttcttt tttccaaaaa gattcaagtg accattttaa ttctctattc
      421 ggacctattc ttggataacc taaattacta ctaattacca taaatatatt atttcatata
      481 taaaaaaacc accgtacaat gcaggtggtc ttagtgcttt agctggaaaa cccataagct
      541 gcatcaaatc ggtaataagg taatatttaa ttttttgatt tgcaagaatt ttatttatag
      601 taaataaatt gaacctagcc ctaaaaaaac aaaaaaacca aagatatccg taattgttgt
      661 taatacaact gttgaagcta tggcaggatc aattcctgtt ttatcaagaa taagcggtat
      721 caaaatacct gacaaacatg caaccattaa atttattatc attgcagcac ctataataat
      781 tccgagtaaa ggcatttgaa accaatatgc tgctacggaa ccgataatta ctgcaaaaat
      841 aatgccgtta attgtaccaa ctgcaaattc ttttccaata actcttaacg cgttagctct
      901 tgttacttca cgcaatgcca gcgctcgtac agctacagtt aatgtttgag taccagcatt
      961 tcctcccatt gatgcaacaa taggcattag aattgcaagt gcaacaagtt gtcttaatga
     1021 tgattcaaaa attccaataa caaacgaagc agctatagcg gtaaataaat tgattaacaa
     1081 ccaaccaaat cgagattgta ttgtttttaa aaccgcgcta tacgtatcat cactttgaac
     1141 tccgcttaaa cgtaataaat cttcttcagt ttcttcctca ataatatcaa taacatcctc
     1201 agatgtaatc tgcccaacaa tttttccaag tttgtctaca acaggtgcag ctataagatt
     1261 gctttgttta aaacctaatg ctatttcttc ttggtctgta tctataggta ttgttttaaa
     1321 ttgcgtgttc attatatttt taaccactgt tgcactagtt gaacgaacaa ttgtatttaa
     1381 atttaaagta ccaattggcc ttcttttgct atctaagata tatacttcat aaatgttctc
     1441 aggtagtatt tctttttttt gtcttaggta tttaagtgtt tttccaattg tccaagaagc
     1501 agagacagaa acaatgtcag ttgccattat acgaccagca gtaacttcag gaaactttaa
     1561 acctttctta acta
//
LOCUS       DTVV01000495            1595 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_409055, whole genome shotgun sequence.
ACCESSION   DTVV01000495 DTVV01000000
VERSION     DTVV01000495.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_409055"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1..1257
                     /gene="fabF"
                     /locus_tag="EYO86_05890"
     CDS             1..1257
                     /gene="fabF"
                     /locus_tag="EYO86_05890"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HIC42596.1"
                     /translation="MSRVVITGLGLLSSIGNNVSETWDNLITGKSGIKKISSFDVSDL
                     PCKIAGYISHNEKDEYYVDRSKYLETREINRNDRFIQYGLIASKMAVEDAGLMALDDK
                     SKLRVGVSVGSGIGGLETIYNGSITLNEKGSRKISPFFIPSSLINLTSGQISIKFGFK
                     GPNHSVVTACATGAHSIGDAAEMIKRGAAEIMIAGGSEAAVCKLGIAGFCAARSLSTK
                     YNDQPTKASRPWDKDRDGFVIGEGAGIVVLEDYENAKKRGAKIYAELIGYGMSGDAHH
                     ITTPSEDGSGGFRAMSEALKMSSVNSDEINYINAHGTSTLIGDDIELSAIMRLTGGND
                     KIKISSTKSSIGHLLGAAGSVEAIITILSIKHNIVPATLNLYNPSHNFKVDLVPHNSI
                     DHKVEIALSNSFGFGGTNAALLFKSI"
     gene            1205..>1595
                     /locus_tag="EYO86_05895"
     CDS             1205..>1595
                     /locus_tag="EYO86_05895"
                     /inference="COORDINATES: protein motif:HMM:PF02618.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42597.1"
                     /translation="MHLDLEEQTLRYYLSLFSVIIFLFYSYYIVHFQFLFNDKHIIKI
                     AKGDSISKISEKIIIDKNYLQKKIYYLLIKFSNKYFSPINYGDFLIEKNSNILSVLKT
                     VSKASNINYEITIIEGWEKYQLTNYLKK"
BASE COUNT          597 a          192 c          286 g          520 t
ORIGIN      
        1 atgagtaggg tagttattac aggtctagga ctactttctt ctataggtaa taatgtctct
       61 gaaacttggg ataatttaat tacaggcaaa tcaggtataa aaaaaatttc ttctttcgac
      121 gtttctgatt taccatgcaa gatagccgga tatatttctc ataatgaaaa agacgaatat
      181 tatgttgata gaagcaaata tttagaaact agagaaataa atagaaatga tagatttata
      241 caatacggtt taatagcttc aaaaatggca gtggaagatg ctggattgat ggcattagat
      301 gataaatcaa aattaagagt tggtgtttca gttggatcag gaataggcgg tttagaaaca
      361 atttataatg gttctattac tttaaatgaa aaaggttcaa gaaaaatatc tccttttttc
      421 ataccttctt ctttaataaa tttaacttcg ggacaaattt caattaaatt tggatttaaa
      481 ggaccaaatc attctgttgt aactgcttgt gcaactggag cacattctat tggagatgca
      541 gctgaaatga ttaagagagg tgctgcagaa ataatgattg ccggaggttc agaagcagca
      601 gtatgcaaac tgggaatagc aggtttttgt gcagctagga gtctttccac taaatataat
      661 gatcaaccca ctaaagcttc aaggccttgg gacaaagata gagatggatt tgtgatagga
      721 gaaggggcgg gtatagtggt gttagaggat tatgaaaatg caaaaaaaag aggtgcaaaa
      781 atatatgctg aactgatcgg ttatggaatg tcaggagatg ctcaccatat tactacacca
      841 agcgaagatg ggtcaggcgg ttttcgagca atgtcagagg cattaaaaat gtcttctgtt
      901 aattcagatg agataaatta tataaatgca catggtactt ctactcttat aggagatgat
      961 atagagttaa gtgcaataat gcgattaact ggtggtaacg ataaaataaa aataagttca
     1021 acaaaatctt caataggcca tttattaggt gcagcgggta gtgttgaagc aataattact
     1081 attctaagta ttaagcacaa catagtacct gcaactttaa atttatataa tccatctcat
     1141 aattttaagg tagatcttgt accacataac tctattgatc ataaagttga aatagcactt
     1201 agcaattcat ttggatttgg aggaacaaac gctgcgttat tatttaagtc tatttagtgt
     1261 aattattttt ttattttatt catactacat tgttcatttt caattcttat ttaatgataa
     1321 gcacataatt aaaatagcaa aaggagatag tataagtaaa atttcagaaa aaataattat
     1381 tgataaaaat tatttacaaa aaaaaatata ttatttatta attaaatttt caaataagta
     1441 tttttctcct ataaattatg gcgatttttt aattgaaaaa aattcaaata tattatctgt
     1501 tttaaaaact gtttcaaaag caagtaatat aaattatgaa atcactatta ttgaaggttg
     1561 ggaaaaatac caattaacaa attatttaaa aaaat
//
LOCUS       DTVV01000496            1948 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_418188, whole genome shotgun sequence.
ACCESSION   DTVV01000496 DTVV01000000
VERSION     DTVV01000496.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_418188"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            120..1334
                     /locus_tag="EYO86_05900"
     CDS             120..1334
                     /locus_tag="EYO86_05900"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIC42598.1"
                     /translation="MKIVVMKFGGTSVANLSKIRDVAKIVEENSQKNKIVVVLSAMSG
                     VTNQLQSYVEEVSSEPSRETDLVITSGEQVTVGLLSMILNREGVKSLPLLGWQVPIVT
                     DENHEKGRILNINNETIYHHFKSNDVIVLTGFQGISVNGVITSLGRGGSDTTAVAIAS
                     SLKAVRCDIYTDVEGVYTADPNVEKNAKKINKVSYEEMLEMSSMGAKVLQTRSVELAM
                     KNNLTLQVLSSLTNTEGTYIVNENKLIEKEIVSGVSYNKNESKITISGIPDKPGISAK
                     IFGLMASNNINVDMIVQNISQDGVSANMTFTVLTKDIELAKKTLERNNNELNYTNLST
                     DSNIAKISVIGMGMMSQSGVAEKMFKTLADKQINILAISTSEIKISVLIDKKYTDLAV
                     KTLHSVYKLDNK"
     gene            1374..>1948
                     /locus_tag="EYO86_05905"
     CDS             1374..>1948
                     /locus_tag="EYO86_05905"
                     /inference="COORDINATES: protein motif:HMM:PF13413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42599.1"
                     /translation="METVGESFKRARKSQEIDLNIVSQDLKISESLLGDIENNQFPSY
                     INVTYLIGHIRSYAKYLNLDEKLLIENFKIQISFDDNNLNNEMRKPNTPVIQFSFFKA
                     LSFVSIIAISSSFYFLFIPSNDLQPEYAMTPNIPENLESTLEEIEMKLSLEKKLIVDT
                     LDNTSQIIADSSQIIEDKDALISSTSAIASLP"
BASE COUNT          723 a          246 c          329 g          650 t
ORIGIN      
        1 gcaattttgg aaaaatgatt aaattctctg ttttttgaag aattattgct cataaaactt
       61 gttactttaa cgtaaaaata ataagagcct ctcaaataat tgaaagtgat attaagtcaa
      121 tgaagatagt agtgatgaag tttggaggta cgtccgttgc taatttatca aagattaggg
      181 atgttgcaaa aatagttgaa gaaaactctc aaaaaaataa aattgttgta gttttatcgg
      241 ctatgtctgg tgttactaat caattacaat catatgttga agaagtgagt tccgaacctt
      301 cacgggaaac agatttggtt attacttcag gagaacaagt aacagttggt cttttatcaa
      361 tgatattaaa tagggaagga gttaaaagcc ttcctttact gggatggcaa gttcctattg
      421 ttacagatga gaatcatgaa aagggcagaa tattgaatat taataatgag actatttacc
      481 atcattttaa aagtaatgat gttattgttt taactggatt tcaggggatt agcgttaatg
      541 gagttattac ctcccttgga agaggtggct cagatacaac cgcagtggca atagcatctt
      601 cattaaaagc tgtacgctgt gatatatata ctgatgtaga aggagtttat actgctgatc
      661 caaatgttga aaaaaatgca aagaaaatta acaaagtatc ttacgaagaa atgttagaaa
      721 tgtcatcaat gggagcaaag gttttgcaaa cacggtcggt tgaattagca atgaaaaata
      781 atttaacttt gcaagtattg tcttctctta ctaatactga aggtacttat attgttaatg
      841 aaaataaatt aattgagaaa gagattgtta gcggtgtttc atataacaag aatgagtcta
      901 agataactat ttcaggtata cccgataaac caggaatatc agccaaaata tttggtttaa
      961 tggcaagcaa taatattaat gttgatatga tcgtacaaaa tatatctcaa gatggagtta
     1021 gtgctaatat gacttttact gttcttacta aagatatcga attagccaaa aaaactttag
     1081 agaggaataa taatgaattg aattacacta atctttctac tgactcaaat attgcaaaaa
     1141 tttcagtaat tggaatggga atgatgtcac aatcaggtgt ggctgaaaaa atgtttaaaa
     1201 ctttggcaga taaacaaata aatattcttg caatttctac ttctgaaatc aaaataagtg
     1261 ttttaattga taaaaagtac actgatcttg ctgttaaaac tcttcatagt gtatacaaat
     1321 tagacaataa gtaataaata gaacgtatta attaattatt aaataactaa attatggaaa
     1381 ctgtaggaga atcttttaag agagccagaa aaagtcaaga aattgatcta aatatagtct
     1441 cacaagattt gaaaatttcc gaaagtcttt tgggagatat agagaataat caatttccaa
     1501 gttacattaa tgttacttac cttattgggc acattcgttc atacgccaag tatctaaatt
     1561 tggatgaaaa attattgata gaaaatttta aaattcaaat ctcatttgat gataataatc
     1621 taaataatga aatgcgcaaa ccaaatacac cggttattca attttctttt tttaaagctt
     1681 tatcatttgt ttcaattatt gctatttctt caagttttta ttttttattt attccatcta
     1741 atgatttaca accagagtat gctatgactc ctaatattcc tgaaaacctt gaatctactc
     1801 tggaggaaat tgaaatgaaa ctttctttgg aaaaaaaatt gattgttgat acattggata
     1861 atacaagtca aataattgcg gattcatctc aaattattga agataaagat gctttgattt
     1921 cttcaacgag tgcaattgca tctttgcc
//
LOCUS       DTVV01000497            1419 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_426116, whole genome shotgun sequence.
ACCESSION   DTVV01000497 DTVV01000000
VERSION     DTVV01000497.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_426116"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            132..848
                     /locus_tag="EYO86_05910"
     CDS             132..848
                     /locus_tag="EYO86_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HIC42600.1"
                     /translation="MGSIAGSKKLKRQMAPKYWGITRKNKRFVITVRPGAHAKSQSIP
                     TAVLLRDTLKKVTTLREAKSVIYNGKINVDGITRKSLHHSIGLMDVITLDGTTDIYRL
                     VPKDGKLLKPLQIDSSEKSKKLVKVTSKVTIKGGKTQLGFHDGRSLITDTDVNVNDSC
                     LIQIPEQKILDVIKLEKNSQVIVIKGVNAGRIGYVDKIKDGSFSLSKRIDLVMDDKKI
                     EIPLKLIMVIGKEKPVIQIR"
     gene            853..1374
                     /locus_tag="EYO86_05915"
     CDS             853..1374
                     /locus_tag="EYO86_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIC42601.1"
                     /translation="MSQQTENMMKKISLNKVVLNMGIGQSGERIETAKAALMQITEGK
                     VNSRHAKKAQRDWGVRKGEPIGVAVTVRGENAILLLKKLLEAKDNMIKEKSFDNEGNF
                     SFGISEHIDIPGVKYDHKIGILGLDAAISLMRPGFSIKLRSKHKAKIGKGHRITKQNA
                     IQFMIKEFGVKTV"
BASE COUNT          621 a          173 c          264 g          361 t
ORIGIN      
        1 gaaacaaaat caaaaaataa tgttaaagca aataacaaaa aggagaataa aaaattagaa
       61 cataaagcaa agccagaagc aaaatcaaaa aataatgtta aagcaaataa caaaaaggag
      121 aataaaaaat aatgggaagt atagcaggaa gtaaaaaatt aaaaagacaa atggcgccca
      181 aatattgggg tataactaga aaaaacaaaa ggtttgttat aacagtaaga cctggggcac
      241 atgcaaaaag tcaatctatc cctaccgctg tcttattacg tgatacacta aaaaaagtaa
      301 ctacattacg agaagctaaa tcagtaatct ataatggaaa aattaatgtt gatggcataa
      361 cacgaaaatc tctacaccac tcgatagggt taatggacgt aataacatta gatggcacta
      421 cagacatcta tagattagtt cctaaggatg gtaaattatt aaaaccactt cagattgata
      481 gctctgaaaa atccaaaaaa ctagtaaaag tcacaagtaa agtaactatt aagggaggaa
      541 aaactcaatt aggttttcat gacggcagat cattaattac agataccgat gtaaatgtta
      601 atgattcttg tttaattcaa attcctgaac aaaaaatttt agatgtgatt aaattagaaa
      661 aaaattctca agttattgtt ataaaaggtg taaatgcggg tagaattgga tacgttgata
      721 aaattaaaga tggctcattt agtttatcaa aaaggattga tttagtcatg gatgataaaa
      781 agatcgaaat tcctctaaag cttatcatgg taattggaaa agaaaagccg gtaatacaaa
      841 ttaggtgata acatgtctca acaaactgaa aatatgatga agaagatttc cctcaataaa
      901 gtagttttga acatgggaat aggacaatca ggtgaacgaa ttgaaactgc aaaagctgcc
      961 ttaatgcaaa ttactgaagg gaaagttaat tcaagacatg ctaaaaaagc acaaagagat
     1021 tggggagtta gaaagggtga acctatcgga gttgctgtaa ctgttagagg agaaaatgca
     1081 atattattat taaaaaaatt attggaagcc aaagataata tgattaaaga aaaatctttt
     1141 gataacgaag gtaatttttc atttggaata tcggagcata ttgacattcc tggtgtgaaa
     1201 tatgatcaca aaattggcat tttagggtta gatgcagcaa tatctcttat gagacctggg
     1261 tttagtatta aactacgaag taaacataag gcaaaaattg gaaaggggca tagaataaca
     1321 aaacaaaacg caattcaatt tatgataaaa gaatttgggg tgaaaactgt ataatggtaa
     1381 aagacagatc atatgaagca actggaagaa aaaaacaca
//
LOCUS       DTVV01000498            1240 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_432475, whole genome shotgun sequence.
ACCESSION   DTVV01000498 DTVV01000000
VERSION     DTVV01000498.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_432475"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(348..929)
                     /locus_tag="EYO86_05920"
     CDS             complement(348..929)
                     /locus_tag="EYO86_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIC42602.1"
                     /translation="MSTKCNIIKKKKIHDGFFKLHELTFTHQKHDGKWSSEIKREIFS
                     GAHVATVIPYDPINKKILLLEQFRSGLLHRKDDPVIIEIVAGIIDEGELPEDAAQREC
                     IEETGCSVKKLKKILSYYPAPGSSESFYHLFLAEVESFKGKRIVGQKDENEDILVGSY
                     GIDEVRNLMKRKKIINGVTLIALQWFFLEYYKN"
     gene            979..>1240
                     /gene="msrA"
                     /locus_tag="EYO86_05925"
     CDS             979..>1240
                     /gene="msrA"
                     /locus_tag="EYO86_05925"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIC42603.1"
                     /translation="MTKALIGAGCFWGIEEYYRKLNGVIDTKVGYSGGKAVKPTYEEV
                     CTGKTGHVEVVQIKFDENMISFQQILNHFWKCHDPTQLNRQGL"
BASE COUNT          430 a          187 c          199 g          424 t
ORIGIN      
        1 agtattggaa tggttaaaaa aaaaataata atgcgtttgt ttttaataat tattttcatt
       61 ttttataata catccatttt tgcagaagtg gatactgatc aatggcaaga cagcaattta
      121 acttataaag atttgattga tcagggattt gaagtgaagg cttatgatat aaatacaatt
      181 acaactgatg taggattaat tttggtattt tttgtaacag tgttacaaaa agaaaaagaa
      241 gtatacgaat gtcaagaata ccaaacattt gatggcaaca tgaaaacttt ggatatgtca
      301 gtggtatgca gagaattaac ccaaccctat aaaagagggg ttaatactta gtttttataa
      361 tattctaaaa aaaaccattg aagagcaatt agggttaccc cattgattat tttttttctt
      421 ttcatcaaat ttctaacttc gtcaatacca taactgccaa cgagaatgtc ctcattttca
      481 tctttttgcc ccacaatacg cttaccctta aaagattcaa cttctgctag aaataaatga
      541 taaaaggatt ctgacgagcc tggagcaggg taataagaaa ggattttttt taacttttta
      601 accgagcatc cagtttcttc aatacattcc ctttgagctg catcctctgg taattctcct
      661 tcatcaatta ttccagcaac aatttctatt attacaggat catcttttct atgaagtaga
      721 cctgaacgaa actgctccaa aagaagaatt tttttattta tgggatcata cggaataaca
      781 gtagcgacgt gcgctccact aaaaatttct ctttttattt cagaactcca cttaccgtcg
      841 tgtttttgat gagtaaaagt taattcatgt aatttaaaaa aaccatcatg gatcttcttt
      901 tttttaatta tattacattt tgtactcata acaacttgtt taaaagataa ttaatttact
      961 ctatatattt aaattaatat gacaaaagct ttaataggtg caggttgttt ttggggaata
     1021 gaagaatatt accgaaaact taatggtgtt attgatacta aggtgggata ttctggaggc
     1081 aaagcagtca aacctactta tgaagaggtt tgtacaggca aaacaggaca cgtggaagta
     1141 gtgcagatta aatttgatga aaatatgatt tcttttcagc aaatactcaa tcatttctgg
     1201 aaatgtcatg atccaactca acttaatcgg caaggattag
//
LOCUS       DTVV01000499            1243 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_439986, whole genome shotgun sequence.
ACCESSION   DTVV01000499 DTVV01000000
VERSION     DTVV01000499.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_439986"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /gene="ftsA"
                     /locus_tag="EYO86_05930"
     CDS             complement(<1..1174)
                     /gene="ftsA"
                     /locus_tag="EYO86_05930"
                     /inference="COORDINATES: protein motif:HMM:PF06723.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIC42604.1"
                     /translation="MKIGILDIGTNKISCFIVNIIKDKEPEILGIGHHQSNGISSGTI
                     IDMELACNSIRSSIQSAENMFGSTVEEFVVNFSSDTIQSHTINLNTSLDNNEVTHEDI
                     KKNYKKIDDFNYGKNRQLLHKIRTSYSIDRNIGIDKPIGMKGNKLIAGFNLITADKNS
                     INNFIKCLSMAKVRISDIVATPYASGLGCLNNDDLYIGTTVIDVGAGCSSIGTFYKNK
                     LVYAGIVNIGGFHISSDIAKGLSTSILAAERLKILHGGSVNFNDENDLIDVPLTGNHE
                     STETHKIHKKELNNIILSRIQEILENIRSKTNKPILERNLTSNIVLTGKSSELKGFIN
                     LTSDFFGIKTRIGKSINIKGLAENTSGPGFSVCCGLIKYKILKEEEYLQINSPLENS"
BASE COUNT          400 a          189 c          147 g          507 t
ORIGIN      
        1 agctgttttc taaaggggaa ttaatttgta aatattcttc ctctttaaga attttatatt
       61 ttattaaacc acaacaaaca gaaaacccag gacccgaagt attttctgca agacctttaa
      121 tattaattga ttttcctatt ctggttttaa ttccaaaaaa atcggaagtt aaattaatga
      181 aaccttttaa ttctgatgat tttccggtta aaacaatatt gctggtcaaa tttctttcca
      241 aaataggttt atttgttttt gatctgatat tctcaagaat ttcttgaatt cttgataaga
      301 taatattgtt caactctttc ttatgtattt tatgagtttc agttgactca tggttaccag
      361 ttaaaggaac atctattaaa tcattttcat cattaaaatt aacacttccc ccatgcaaaa
      421 tttttaatct ttctgctgct aaaattgaag ttgataaacc ttttgcaata tcagatgaaa
      481 tatgaaatcc tccaatatta actatgcctg catatacaag tttattttta taaaaggttc
      541 ctatagagga acaacccgcg ccaacatcta taactgttgt tcctatataa agatcatcat
      601 tatttaaaca tcctaagcct gaagcataag gagttgcaac aatatcgcta attcttactt
      661 tagccatgga taaacattta ataaaattat tgatggaatt cttatctgca gttattagat
      721 taaaacccgc aattaattta tttcccttca taccaattgg tttgtctatt ccaatatttc
      781 tgtcgatact ataagaagtt ctgattttgt gtaaaagctg tctatttttt ccataattaa
      841 aatcatctat ttttttgtaa ttttttttaa tatcttcatg agttacttca ttattatcta
      901 aagaagtatt taagtttata gtatgtgatt gaattgtatc tgaggaaaaa tttacaacaa
      961 attcttctac tgtacttcca aacatatttt ctgctgattg aattgaagat cttattgaat
     1021 tacaagctaa ttccatgtct ataattgtgc ctgaagatat accattggat tgatgatggc
     1081 ctatacctaa tatctcaggt tctttatctt ttattatatt tactataaaa caagatattt
     1141 tgttcgttcc tatgtcaagt atgccaatct tcatttttta tttttcattg caattttttt
     1201 tgtattcatt tttataaata tttttttttc aaagaatctt aag
//
LOCUS       DTVV01000500            1053 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_444574, whole genome shotgun sequence.
ACCESSION   DTVV01000500 DTVV01000000
VERSION     DTVV01000500.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_444574"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="EYO86_05935"
     CDS             complement(<1..198)
                     /locus_tag="EYO86_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42605.1"
                     /translation="MKFNKLKVKGFKSFVEPTEIIIENGLTGIVGPNGCGKSNLVEAF
                     RFVMGELSPKQMRGSELDDVIF"
     gene            complement(205..816)
                     /locus_tag="EYO86_05940"
     CDS             complement(205..816)
                     /locus_tag="EYO86_05940"
                     /inference="COORDINATES: protein motif:HMM:PF13462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIC42606.1"
                     /translation="MCNLYRILSIVFFIVFFILPISATTVLEVTEDDFVVGDKNAPVT
                     IIEYASLSCSHCADFHNNTLEDLIKEYVDTGKARIVFRDFPFNYPALIGSMVLRCIPE
                     DVRYDYMNALFQLQPKWVVRENAKSTQELYKIMQSGGMTKEEFETCTNNVELENTILQ
                     ALIAAQNEFNIQSTPSFLINGNLVEGNKSIKEFRQIIDKILSE"
BASE COUNT          336 a          185 c          167 g          365 t
ORIGIN      
        1 aaaaataaca tcatctaact cacttcctcg catttgttta ggggaaagct cacccatcac
       61 aaagcgaaaa gcttctacaa gattggattt tccacagccg tttggaccca caatacccgt
      121 gagcccattt tcaataataa tctctgttgg ctcaacaaac gatttaaagc ccttaacttt
      181 aagcttatta aattttatca ttaactattc tgataatatt ttatcaatga tttgtctaaa
      241 ttcttttata gatttatttc cttccactaa attaccgttt attaaaaagg agggcgttga
      301 ttggatatta aattcatttt gagcagcaat aagagcttga agaatagtat tttccaattc
      361 aacattgttg gtacaagttt caaactcttc ttttgtcatg ccaccgcttt gcataatctt
      421 atacaattct tgtgttgatt tggcgttttc acgcacgacc cattttggtt gtagttgaaa
      481 taaggcattc atataatcat agcggacatc ttcaggaatg cagcggagaa ccatactgcc
      541 tattaaagcc ggataattaa agggaaaatc acggaacacg atccgcgctt ttcccgtatc
      601 aacatattct ttaattaagt cctcaagcgt attattgtgg aaatcggcgc agtgactgca
      661 tgataatgag gcatattcta taatagtaac gggagcattt ttatcgccaa caacaaaatc
      721 atcctctgtc acttccaaaa ctgtggttgc agatatgggc aatataaaaa aaactataaa
      781 aaagacaatt gataaaatac gatataaatt gcacatgatt atttatcttc gttactaatg
      841 gataatccta aattcacaat agattcttca agttgtttat tggacaatgt aggtgcagaa
      901 cgtttgatag ctaatttttt ttcttttgca tcaatgaggt tgacatttcg ttgttttaaa
      961 tcgtactgat cggttttgac aagaagttga tgtattttaa ttcctttaat tgctttgtaa
     1021 ccaaaataag aattaatttt ttctatgatt ttg
//
LOCUS       DTVV01000501            1506 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_464406, whole genome shotgun sequence.
ACCESSION   DTVV01000501 DTVV01000000
VERSION     DTVV01000501.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_464406"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            23..391
                     /gene="rplN"
                     /locus_tag="EYO86_05945"
     CDS             23..391
                     /gene="rplN"
                     /locus_tag="EYO86_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIC42607.1"
                     /translation="MIQMQSNLFVADNSGARKIQCIKVLGGSKRRFASIGDIIVVSVK
                     DAIPRGKVKKGELFKAVIVRTKKNFSRADGTSIRFDKNAAVLLDKQEEPIATRIFGPV
                     TRELRTKKFMKIISLAPEVL"
     gene            391..705
                     /gene="rplX"
                     /locus_tag="EYO86_05950"
     CDS             391..705
                     /gene="rplX"
                     /locus_tag="EYO86_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIC42608.1"
                     /translation="MNNIKLKKGDEVIVLTGKDKGKIGKIVLVKKQANKAVVAGINKV
                     KKHQKPDNNQAGGIIEKEMPINFSNLAYYDSISKKGVKLGYKFNEKKEKVRIIRNSGK
                     EI"
     gene            708..1259
                     /gene="rplE"
                     /locus_tag="EYO86_05955"
     CDS             708..1259
                     /gene="rplE"
                     /locus_tag="EYO86_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIC42609.1"
                     /translation="MSRLSEDYKNTIKNKMMEKFHYRNMYQIPKIYKIVLNMGIGEAK
                     EDEKILDKAQKELALISGQRPVKTRAKKAIANFKIREGMPLGVSVTLRNKIMYEFLDR
                     LINIAIPRIRDFRGLNPKSFDGNGNYTVGLKEQIIFPEINYDKIDKIRGLNITICTTS
                     SSNAEALELLKNFNMPFWALETN"
     gene            1267..>1506
                     /gene="rpsN"
                     /locus_tag="EYO86_05960"
     CDS             1267..>1506
                     /gene="rpsN"
                     /locus_tag="EYO86_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012461618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIC42610.1"
                     /translation="MAKQSSIQKNLNRKNIVSKFKNRRHALKKKIMQKNLSMEERFKL
                     QTKLNDLPRDSAKARVRNRCELTGRSRGTYRKFGLS"
BASE COUNT          648 a          168 c          270 g          420 t
ORIGIN      
        1 gctgttggag gagataaata atatgatcca aatgcaatca aatttatttg tagctgacaa
       61 ttcaggggct agaaaaattc agtgcatcaa ggtgctagga ggatcaaaaa gacgttttgc
      121 ttcaattggt gatatcatag tggtatctgt taaagatgct ataccaagag gaaaagtaaa
      181 aaaaggcgag ttattcaaag ctgttatagt tagaacaaaa aaaaatttta gtagagctga
      241 tggaactagt attagatttg ataaaaatgc cgcagtactt ttagacaagc aggaagaacc
      301 aattgcaaca agaatttttg gacctgtaac aagagaattg agaacaaaaa aatttatgaa
      361 aataattagt ttagctccag aagtgcttta atgaataata ttaaattaaa aaaaggtgat
      421 gaagttattg ttcttacagg taaggataaa ggtaaaattg gaaaaatagt tttggttaag
      481 aagcaagcca ataaggcagt tgttgctggt ataaataaag ttaaaaaaca tcaaaaacca
      541 gataataatc aagctggcgg aattatagaa aaagaaatgc caataaattt ttcaaacttg
      601 gcatactatg attctatatc aaaaaaaggt gttaaacttg gttataaatt taatgaaaaa
      661 aaagaaaaag ttcgaattat tagaaattca ggaaaagaaa tatgaatatg tcaagattat
      721 cagaagatta taaaaacact ataaagaata aaatgatgga aaaatttcat tacagaaata
      781 tgtatcaaat tccaaaaata tacaaaatag ttttgaatat gggtattgga gaagcaaaag
      841 aagatgaaaa aattctagat aaagctcaaa aggaactggc acttatttct ggacaaagac
      901 ctgtgaaaac aagagcaaaa aaagcaattg caaactttaa aattcgtgaa ggaatgccat
      961 tgggtgtttc cgtaacactt cgaaacaaaa tcatgtatga atttttggat aggctaatca
     1021 atatagcaat tccaagaatc cgtgatttta gaggtctaaa tcctaaaagc tttgatggaa
     1081 atggtaacta tacagtaggt ttaaaagagc aaattatttt tcctgaaatt aattatgata
     1141 aaattgataa aattcgagga ttaaatatta caatatgcac tacttcttct agtaatgcag
     1201 aagcattaga actgttaaaa aactttaata tgcctttttg ggcattagaa acaaattagg
     1261 tagattatgg ctaagcaaag ttcaattcaa aaaaatttaa atagaaaaaa tattgtatct
     1321 aaatttaaaa atagacgtca tgcattaaag aaaaaaatta tgcaaaagaa tttatcaatg
     1381 gaagaacgat ttaaattaca aacaaaatta aacgatcttc ctagagatag tgcaaaagca
     1441 agagttcgca atcgatgtga gctaacgggt cgcagtagag gaacttatag aaaatttggc
     1501 ctttca
//
LOCUS       DTVV01000502            1777 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_473028, whole genome shotgun sequence.
ACCESSION   DTVV01000502 DTVV01000000
VERSION     DTVV01000502.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_473028"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            86..1243
                     /locus_tag="EYO86_05965"
     CDS             86..1243
                     /locus_tag="EYO86_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIC42611.1"
                     /translation="MQVSKRLFSLETESAFAILEKVKKLELEGKDIINLGIGQPDFPT
                     PQNILEAAKKAITDGHHGYTPSNGILPLREAVSKKIYEDYNTSVSPDNILITPGGKPV
                     IFFAALIFGEPGVEIIYPDPGFPIYRSMIKFTGAKPVPLELKEKDNFEVNIELLEKLI
                     TNKTRLIILNNPNNPTGSFMNETKISQLVNILEKYPETVILSDEIYSKIIFDNFSMPS
                     LLKFESIRDRLIVLEGWSKTFCMTGWRLGWSVWPNKIIDFANKLCVNDHSCPSSISQY
                     AGLEALMGPQDEVVKIVKEFERRKSFVHNGLNRLKKIKCFKPGGAFYAFPNIIETNMS
                     GNQFADIALNELGVALVPGSSFGDSVDEYVRLSYANSMENIEKAIYRLSKI"
     gene            1240..1578
                     /locus_tag="EYO86_05970"
     CDS             1240..1578
                     /locus_tag="EYO86_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIC42612.1"
                     /translation="MKKIEAIIKPLRLSIVKEALHEIGLSGMTVLDVKGFGRQRGQDG
                     LDINQDYDDEFHAKIKIELIVEDTMVEQAIESIKNTAYSGKIGDGKIFISSIEQVIRI
                     RTGEKDSDAV"
BASE COUNT          644 a          237 c          333 g          563 t
ORIGIN      
        1 gcccgcgctc taaccaactg agctacgttc gccattttgc ataaaaatat atttatttta
       61 tgctgaattt cgatatacca aaataatgca agtttccaaa aggttattta gtttagaaac
      121 agagtccgca tttgccatat tggaaaaagt aaaaaaacta gaattagaag gtaaagatat
      181 aataaatctt ggaatagggc agcctgattt tccaacacct caaaatatac tagaggcagc
      241 aaaaaaagcg attactgatg gtcatcatgg gtatactccc tccaatggaa ttttgccttt
      301 aagagaagct gtttccaaaa aaatatatga agattataat acttcagtta gtcctgataa
      361 tattcttatt accccgggtg gaaaaccagt aatttttttt gctgcattaa tttttggtga
      421 acctggagtt gaaataattt atcctgatcc tgggtttcct atctatcgct ccatgatcaa
      481 gtttactgga gcaaagcctg ttcctttaga actaaaagaa aaagataatt ttgaagtaaa
      541 tatagaacta cttgaaaaat taattacaaa taaaacacga ttaattattt taaataatcc
      601 aaataatcca actggctctt tcatgaatga aacaaaaatt tcacagctcg tgaatatttt
      661 agaaaaatat cctgaaacag tgattttaag tgatgaaatt tatagtaaaa taatttttga
      721 taatttctct atgccgtcat tattaaaatt tgaatccatt agggatcggc taattgtttt
      781 agaaggctgg agtaaaactt tttgtatgac aggttggcgc ttgggttgga gtgtttggcc
      841 taataaaata attgattttg cgaataaact ttgtgttaat gatcattctt gcccatcatc
      901 aatttctcaa tatgctggtt tggaagcttt gatgggacct caggatgaag ttgtgaaaat
      961 tgtaaaagaa tttgaaagaa gaaaatcttt tgttcataat gggctaaata gattaaaaaa
     1021 aattaaatgc tttaaacctg gtggtgcttt ttatgcattt cctaatatta ttgaaactaa
     1081 catgtctggt aatcaatttg cggatattgc attaaatgaa ttgggagttg ctcttgttcc
     1141 tggatcaagt tttggggaca gtgtagatga atacgttaga ttaagttatg ctaattctat
     1201 ggaaaatatt gaaaaagcta tttataggct ttctaaaata tgaaaaaaat tgaagcaata
     1261 ataaaacctt taagattatc tattgtaaaa gaggcactgc atgaaattgg cttatcggga
     1321 atgacggtgt tagatgttaa aggttttggt aggcaaagag gtcaggacgg attggatatc
     1381 aatcaagatt atgatgatga gtttcatgct aaaattaaaa ttgagttaat tgttgaagac
     1441 acaatggttg aacaagcaat agaatcaata aaaaatactg cttattcagg taaaattggt
     1501 gatggtaaaa tttttatatc aagtatcgag caagtgatta ggattaggac aggagaaaaa
     1561 gacagtgatg ctgtctaatt tttctttaca aaacaataga aataaataaa aggatcttaa
     1621 tcgttcaact catcataact gatgggtcgg aagtagacat tagtcgaagg aacgcatgca
     1681 aaagttataa atcgttcaac ccaaaagggt cggaagtaag caacagctga aggaacgcgc
     1741 ataaaagcag atttcataac tagagcatga atttaac
//
LOCUS       DTVV01000503            1446 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_474961, whole genome shotgun sequence.
ACCESSION   DTVV01000503 DTVV01000000
VERSION     DTVV01000503.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_474961"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="EYO86_05975"
     CDS             <1..653
                     /locus_tag="EYO86_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIC42613.1"
                     /translation="GIDCIDLLQLHCPPSEICPKKETYEMMDEIVKLGKIANYGVSVE
                     KVSEAMDAIQFPNVKSIQIIFNIFRQKPAETFFKEAAKRNVGIIARVPLASGLLTGKM
                     NQESTFPQNDHRNYNINGDAFDVGETFSGVNFEKGLKAVEKLKDLLPDNFSLTDLALK
                     WILMHDEVSAVIPGAKNKSQVLKNVQASDLEDISSLLPKINSIYDEFIKPDVHNRW"
     gene            718..>1446
                     /locus_tag="EYO86_05980"
     CDS             718..>1446
                     /locus_tag="EYO86_05980"
                     /inference="COORDINATES: protein motif:HMM:PF04107.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIC42614.1"
                     /translation="MINKKKLIEYFVKGIKSDTELKIGTEHEKFILNKSTLKTLSYDE
                     KNGIRDVFLSLIKLGWKPTYEMKNNTIVGLKNGQQNISLEPAGQFELSGQPLENIHQT
                     CDEITNHLNQMKQLSIKHNFILLGMGVEPTLDLKDFSWMPKERYTIMREYMPNVGKNG
                     LDMMQRTCSSQVNLDFSSETDMIKKFRVLLSLESIGTAIFANSPFASETLTDFKSLRS
                     VYWMNTDNQRTGIIPFVFDYNFNFE"
BASE COUNT          530 a          210 c          256 g          450 t
ORIGIN      
        1 taggaattga ttgtattgat ctcttgcaat tgcattgccc tccaagtgaa atttgtccta
       61 aaaaagaaac atacgaaatg atggatgaga tcgttaaact aggcaaaata gctaactatg
      121 gtgttagtgt tgaaaaagta tcagaagcca tggatgctat tcaatttcca aatgtaaaaa
      181 gtattcaaat tatatttaat atatttaggc aaaaacctgc tgaaactttt tttaaagaag
      241 ccgcaaagag aaatgtcggt attattgcaa gagtcccgct tgcaagtggg ttgctaactg
      301 gaaaaatgaa tcaagaatca acatttcctc aaaatgatca tcgaaattat aatattaatg
      361 gagatgcttt tgatgtcgga gaaacattct caggtgtaaa ttttgaaaaa ggtcttaagg
      421 cggttgaaaa attaaaagac ttacttcctg ataatttttc attaacagat ttagcactga
      481 aatggattct aatgcatgat gaggttagtg ctgttattcc aggagcaaaa aataaatcgc
      541 aggttctaaa gaatgtacag gcatcagatt tagaagatat ttcatcttta ttaccaaaaa
      601 ttaattctat ttatgatgag tttattaaac ctgatgttca taaccgctgg taataattga
      661 atatagtttc tcaacatccc tcgtgattgg tagtagaatt atgtcatttg aaaattaatg
      721 ataaataaaa aaaaactaat tgaatatttt gtaaaaggaa tcaagtctga tactgaatta
      781 aaaatcggaa cggagcatga aaaattcatc ttaaataaat ctaccttaaa aactctttct
      841 tatgatgaaa aaaatggaat acgcgacgtt tttctaagtt taataaagtt aggctggaaa
      901 cccacttatg agatgaaaaa taatacaatt gtaggtttaa agaacggtca acaaaacata
      961 agtctagagc cagctggtca gtttgaactt tcaggtcagc cgttagagaa tatccaccag
     1021 acttgtgatg aaatcactaa tcaccttaat caaatgaaac aattatctat aaaacacaac
     1081 tttatattgc ttggcatggg tgtagagcca acactggatc taaaagattt ctcctggatg
     1141 ccgaaggaac gctatactat catgcgggaa tatatgccta atgtaggcaa gaatgggttg
     1201 gacatgatgc aaaggacatg ttcctcacaa gtgaaccttg atttttcatc cgaaacagac
     1261 atgattaaaa aattccgcgt attattgtct ttagaatcta ttggtacagc tatttttgcc
     1321 aattcaccat ttgccagtga aacattaact gattttaaga gtttacggtc agtatactgg
     1381 atgaatactg ataatcaaag aacaggaatt ataccatttg tatttgatta taattttaat
     1441 tttgaa
//
LOCUS       DTVV01000504            3244 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_493564, whole genome shotgun sequence.
ACCESSION   DTVV01000504 DTVV01000000
VERSION     DTVV01000504.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_493564"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(109..327)
                     /gene="infA"
                     /locus_tag="EYO86_05985"
     CDS             complement(109..327)
                     /gene="infA"
                     /locus_tag="EYO86_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIC42615.1"
                     /translation="MPKEGTIEFQGVVLELLPNAMFKVKLENDHEILAHSSGKMRKNR
                     IRVLAGDKVTVEMTPYDLTKGRITFRWK"
     gene            complement(360..1655)
                     /gene="hisD"
                     /locus_tag="EYO86_05990"
     CDS             complement(360..1655)
                     /gene="hisD"
                     /locus_tag="EYO86_05990"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIC42616.1"
                     /translation="MKILKSENQNFFKDLKFFLQKRLQQNIKDIDESVKKIINQIIQN
                     GDEALFKYTKKFDGSLIDSSNVLISKKIRESYKNKADINVLQSFKVAIQNITKYHEKQ
                     KPQNYEIIEDGAKTSSIWKPIQSVGLYVPGGNAVYPSSLIMNAIPAKVAGVERIVVVT
                     PSQSGSLNPYILALLNELNIKEVYQIGGAHAIAALAYGTESVKPVNKIFGPGNAYVAS
                     AKRQVFGKVGIDLIAGPSEIVVVADKDNKPNWVAADLIAQSEHDEKAQSILITDDENF
                     SNQVILSINKLKEQLPRKEIIDKSLNNNGLIIIVNNFDNVADIIDFIAPEHLHLQNKS
                     RNKILEKVNDVGGVFMGEYASEVFGDYIIGTNHVLPTSGSAKFSSGLGVLDFMKRSSV
                     VEMNLESFNKNQDNVSKMASIENFEGHKLSVKIRQMDKK"
     gene            complement(1652..2305)
                     /locus_tag="EYO86_05995"
     CDS             complement(1652..2305)
                     /locus_tag="EYO86_05995"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIC42617.1"
                     /translation="MMTRKIIIAVPRGRIIKACKKILNKTTFAPDPLLFDEKTRKLTF
                     KSKNPNINYIKVRAFDVCTFVAFGAAHIGIAGDDVVQEYDYSEVYAPLNLNIGHCRLS
                     IAAPINLLKNEDPSSWSNIRVATKYPNLTKRFFADKGVQVDPIKLNGSMELAPSLSMC
                     RRIVDLVSTGKTLKENGLKEVETIIEVQSKLIINRTAYKTKIKIIESLINEISKIVS"
     gene            complement(2362..>3244)
                     /locus_tag="EYO86_06000"
     CDS             complement(2362..>3244)
                     /locus_tag="EYO86_06000"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIC42618.1"
                     /translation="IHLDGLSKLGVDFEIDNGFVKGRVKGKLTGTTITLTFPSVGATE
                     NIMMAAAQADGISIINNAAQEPEIIDLAVCLNKMGAKISGAGSNSIKIVGVNDFYQAD
                     HSIMTDRIVAGTYTIAAVMLNKEFTVKNINSNHLNSLIETLKSMGANLKIDESSITIL
                     PSNKIYGTNIETAPYPGFPTDLQAQVMALMSLAEGTSQITEKIFENRFMHVSELNRLG
                     ANIKIKNDTAYIEGGRKFKGAQVMASDLRASVSLVLAALCAEGETQVNRVYHLDRGYE
                     KIEETLGKCGPSIIREK"
BASE COUNT          978 a          594 c          435 g         1237 t
ORIGIN      
        1 atttttttga acaaaatggt atgaatggat cttttgattt tttcttgcaa gaagggcact
       61 taacttttgt tttgtgaagc ttaactatct ttttagaata aagtttattt atttccatct
      121 aaaggttatt cggcctttgg ttaaatcata tggtgtcatt tcaactgtaa ctttatctcc
      181 tgccaaaaca cgaattctat tttttctcat tttgcctgat gaatgagcga gtatctcatg
      241 atcattttct aattttacct tgaacattgc attaggcaaa agctctaata caacgccttg
      301 gaactcaatc gtaccttctt ttggcattaa ttctccattt attatttcaa gttatataat
      361 tattttttat ccatttgtct aattttaaca gatagcttgt gaccttcaaa attttcgatg
      421 ctcgccatct ttgaaacatt atcttgattt ttgttaaaac tttctaaatt catttcaact
      481 actgaacttc gtttcataaa atctaacaca ccaagacctg aagaaaattt tgctgaacct
      541 gaagttggca aaacatggtt tgtgccaatt atataatctc caaacacttc agaagcatat
      601 tcacccataa atactccgcc aacatcgtta actttctcta atattttatt tcgagattta
      661 ttttgtaaat gtaagtgttc tggcgcaata aaatcaataa tatcagcaac attgtcaaaa
      721 ttatttacaa taattattaa accattatta tttaaacttt tatcaattat ttcttttctt
      781 ggaagttgtt cttttaattt gttgattgac aagattactt gattggaaaa gttttcatca
      841 tcagtaatta atattgattg agctttttca tcatgttctg actgagcaat caaatctgca
      901 gctacccaat taggcttatt atctttatca gctacaacaa caatttctga aggccccgct
      961 attaaatcta taccgacctt accaaatact tgtctttttg cactagctac ataagcattt
     1021 cctggtccaa aaattttatt gactggtttt acactttccg tgccataagc tagtgctgca
     1081 atcgcatgtg cacctccaat ctgataaacc tcttttatgt ttagttcatt taacaatgct
     1141 aaaatataag gatttaatga tcctgactga cttggagtaa caacaacaat tctttccact
     1201 ccagcaactt ttgctggtat ggcattcatt ataagtgatg aaggataaac cgcatttcca
     1261 cctggtacat aaagcccaac tgattgaatt ggtttccata ttgatgaagt ctttgctcca
     1321 tcttcaatga tctcgtaatt ttgtggtttt tgtttttcat gatattttgt aatattttga
     1381 atagctacct taaaagattg taatacattt atatcagctt tatttttata agattcacgg
     1441 attttttttg aaattaatac attagatgaa tcaattaaac ttccatcaaa ttttttagta
     1501 tacttaaaaa gagcctcatc gccattttga ataatttgat taatgatttt ctttaccgat
     1561 tcatcaatat ctttaatgtt ttgttgtagt cttttttgta aaaaaaattt aagatcttta
     1621 aaaaaattct gattttcaga ttttagaatt ttcacgatac aatcttgcta atttcattaa
     1681 tcaaggattc aattattttt atttttgttt tgtaagcagt tctgttaata attaattttg
     1741 actgtacttc aattattgtt tcaacttctt ttaatccatt ctccttcagc gttttcccag
     1801 tagaaaccag atctactatt cttctgcaca ttgataaaga tggtgcgagc tccatggaac
     1861 cgtttaattt aattggatca acctgtactc ccttgtcagc aaaaaaccgt ttggtcaaat
     1921 tgggatactt tgtggcaaca cgaatattac tccaacttga tggatcttca tttttcagca
     1981 agttaatagg tgctgctatg gataatctac agtgaccaat atttaaattt aaaggagcat
     2041 atacttcaga ataatcatat tcttgcacaa cgtcatcccc agctatccct atgtgagctg
     2101 ctccaaaagc aacaaaagta cacacatcaa atgctcttac tttaatataa tttatgtttg
     2161 gattcttaga tttaaaagtt aattttcttg ttttttcgtc aaacaaaaga ggatcaggtg
     2221 caaatgttgt tttattaaga atcttcttac atgctttaat aattcttcct cgaggaacgg
     2281 ctatgataat tttcctagtc ataataaata atttgtaggc tgttctatac acctcaacaa
     2341 aaacttctat aaaaagttaa tttatttttc tctaattatt gatggtccac attttccaag
     2401 agtttcttca attttttcat atcctctatc aagatgataa acgcgattta cttgcgtttc
     2461 tccctcagca cacaaagccg ctaaaaccaa actaacgctc gctctcaagt cgctggccat
     2521 tacttgtgct cccttaaatt ttctaccgcc ttcaatgtaa gctgtatcat tttttatttt
     2581 tatatttgcc cctaaacggt taagttcaga cacatgcata aaacgatttt caaatatttt
     2641 ttcagtgatt tgagatgttc cttctgccag agacattaaa gccattactt gcgcctgcag
     2701 atctgttgga aagcccgggt aaggtgcagt ttctatattt gttccataaa ttttatttga
     2761 aggtaaaatt gttatagaac tttcatctat ttttaaattc gcacccattg attttaaagt
     2821 ttcgatcaga gaatttaaat ggttggagtt aatattttta actgtaaatt ccttatttag
     2881 cataacagcc gctatagtat atgttccagc cacaattcta tcagtcataa ttgaatggtc
     2941 agcttgataa aagtcattta cgccgacaat tttaatagaa ttactaccgg ctccagatat
     3001 cttagctccc attttattta agcaaacagc taaatcaatt atttctggtt cttgcgctgc
     3061 attattaatt attgaaattc catctgcttg cgcagcagcc atcataatgt tctcagtagc
     3121 tccaacactc ggaaacgtta gggttatggt tgtgccagtt aattttcctt taactctgcc
     3181 ctttacgaat ccgttatcta tttcaaaatc aacacccaat ttacttaatc catctaaatg
     3241 aata
//
LOCUS       DTVV01000505            1291 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_501328, whole genome shotgun sequence.
ACCESSION   DTVV01000505 DTVV01000000
VERSION     DTVV01000505.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_501328"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /gene="secD"
                     /locus_tag="EYO86_06005"
     CDS             <1..1015
                     /gene="secD"
                     /locus_tag="EYO86_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIC42619.1"
                     /translation="NDRILIELPGLADPNRIKNLLGKTANLTFRLVSETEDSFGSELL
                     FYEDDDGQLNISKRVILSGDNLINAQPKLDREINEAVVSFTFDRTGSKKFARATSSNV
                     GKRMAIILDNKIISAPVIREAILGGNGQITGDFTFQSATDLALLLRSGALPAPLNIIE
                     ERTVGPDLGEDSIKAGAISLAIGFLLVIGYMLFKYKFLGIIADLALIINLILLIGILT
                     IFEATLTLPGIAGIILTVGMAVDANVLIFERIKEEMKIEKSNIHAFDMGYKRAQSAIL
                     DANITTLISAVILFFLGSGPVKGFALTLGIGVLTTLFCAYFFARHLSSLYVMRNRDKQ
                     IVI"
     gene            1012..>1291
                     /locus_tag="EYO86_06010"
     CDS             1012..>1291
                     /locus_tag="EYO86_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIC42620.1"
                     /translation="MIFRVSNDIKFSHFYRKLNILSLIFIIFSILVLLFKGLNLGVDF
                     KGGTLIELRTENSMTNIAEIRQSFLKMNLGDVTVKKFGKENDYLAKIEI"
BASE COUNT          434 a          170 c          236 g          451 t
ORIGIN      
        1 taatgataga attttgattg agttgccagg gctagctgat cctaatagaa taaaaaattt
       61 attggggaaa actgcaaatt taacttttag attggtttct gaaactgaag attcttttgg
      121 ttctgaactt ttattttatg aagatgatga cggtcaatta aatattagca aaagagttat
      181 tttgagtgga gataatctga ttaatgctca accgaaactc gatagagaaa ttaatgaagc
      241 ggtagtaagt tttacctttg acagaactgg atcaaaaaaa tttgctaggg ctacttctag
      301 taatgttggt aaaagaatgg caataatttt agacaataaa attattagcg caccagtcat
      361 cagagaggca atattaggtg gtaatggaca aattacaggt gactttacat ttcaatcggc
      421 gactgattta gcattattgc ttagatcagg agcattaccg gcaccactta acatcattga
      481 ggaacggaca gttggtcccg atcttggcga agactctatt aaagccgggg ctatttcgtt
      541 ggccataggt ttcttattag tcataggtta tatgcttttt aaatataagt tcttaggaat
      601 tattgctgat cttgctttaa tcattaatct tattctttta attggtattt taacaatttt
      661 tgaagccaca ttaactctac caggaattgc tggaatcatt ctaacagttg gtatggcagt
      721 agatgcaaat gtgttaattt ttgaaagaat aaaagaagaa atgaaaattg aaaaatctaa
      781 tattcatgct tttgacatgg gttacaaaag agcacaatca gcaatcctag atgcaaatat
      841 aacaacctta atatcagcag ttatactttt ctttctaggg tccggtccag ttaagggttt
      901 tgctctgact cttggaatag gtgttttaac aactttattt tgtgcatatt tttttgctcg
      961 tcacttatca tcactttatg tcatgagaaa tagggataaa caaatcgtaa tatgattttc
     1021 agagtgtcta atgatattaa attttctcat ttttacagaa aactgaatat tctgtcatta
     1081 atttttatta ttttttctat tcttgttctt ttgtttaaag gactgaattt aggggtcgac
     1141 tttaagggag gcactcttat tgagctaaga acagaaaatt caatgaccaa tatagctgaa
     1201 ataagacaat catttttaaa aatgaattta ggagatgtta ctgtaaaaaa atttggtaaa
     1261 gaaaatgatt atttagcaaa aattgaaata a
//
LOCUS       DTVV01000506            2275 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_537758, whole genome shotgun sequence.
ACCESSION   DTVV01000506 DTVV01000000
VERSION     DTVV01000506.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_537758"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(107..949)
                     /locus_tag="EYO86_06015"
     CDS             complement(107..949)
                     /locus_tag="EYO86_06015"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010297913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HIC42621.1"
                     /translation="MNWLTNFVKPKLSALVNRREVPENLWQNCPSCANMLHHKDLNEN
                     LRVCNTCNYHFRMSAEKRIEILFGDNNFKEIELNIIPDDPLKFNDKKKYIDRLKEYRK
                     KTNRNDAFILGKGDINQKNAIFGFMNFNFMGGSMGRAVGDGIVLGAKKAIENKCPYII
                     FTSSGGARMQEGIISLMQMPRTVAAVELLNQNKIPYIVVLTEPTTGGVTASFAMLGDI
                     TIAEKGSTIGFAGKRVIQDTIREELPIDFQKAEYLHEHGMVDIIMHRKDLKKTLSDIL
                     HHIS"
     gene            complement(946..1740)
                     /locus_tag="EYO86_06020"
     CDS             complement(946..1740)
                     /locus_tag="EYO86_06020"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIC42622.1"
                     /translation="MSERIKKVFSDKKKKLITFVTGGDPDKKTSQKILEKIVECGSDV
                     VEIGMPFSDPMADGPTIQLSSNRAINNGTNLETIFNICKNIRKINNAIPIVLMGYYNV
                     IFHFGVKRFVCECSKIGIDGLIIVDLQPEEDPELFDEIQFHDIDLIRLITPTTDPKRL
                     KKIIQHASGFLYYVTITGITGQKSADMKELKHSVKEIRRQSNLPIVAGFGIKDKNQVS
                     EICAITDGAVVGSSIVKIIENNLNQSDVILKLIGDFVKELKKGTNQ"
     gene            complement(1740..>2275)
                     /locus_tag="EYO86_06025"
     CDS             complement(1740..>2275)
                     /locus_tag="EYO86_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIC42623.1"
                     /translation="EYENKLPDLLVACIGGGSNALGLFYDFLDDNEVKMLAVEAAGLG
                     LKSGKHAASLSGGQPGVLHGNKTYLLQSDSGQIEEAHSISAGLDYPGIGPEHSFLFDE
                     KRVQYVSATDEEALKAFQYCCHKEGIIPALEPAHALAALIKIAPKMNSDEIVVMNMCG
                     RGDKDIFTVAKELNIKL"
BASE COUNT          699 a          412 c          319 g          845 t
ORIGIN      
        1 attttttgta gtaattccaa taacctgttt agagataaat cgatatattt cggatgtaga
       61 gtgattagtt gttgtaacaa cttgtctagt tgtttaggca ttttgattaa gaaatgtgat
      121 gaagtatgtc ggataaagtt tttttaagat ccttgcggtg cataataata tctaccatcc
      181 cgtgttcatg taaatattct gctttttgaa aatcaattgg aagttcttct cttatagtat
      241 cttgaataac cctttttcct gcaaatccaa ttgttgatcc tttttcggca atagtaatat
      301 caccaagcat ggcaaaagat gctgtcactc ctcctgtggt aggttcagtt aaaacaacga
      361 tatatggaat tttattttga tttaatagtt ccaccgcagc tacagttcgt ggcatttgca
      421 ttaggcttat tattccctcc tgcattcgtg caccacctga ggatgtaaaa attatatacg
      481 gacacttatt ttctattgct ttttttgcac caagaacaat tccatctcca acagctcttc
      541 ccattgatcc acccatgaaa ttaaaattca taaatccaaa tattgcattt ttttgattaa
      601 tatcgccttt ccccaaaata aaggcatcat tgcgattggt tttttttcta tattctttta
      661 atctatctat atattttttt ttatcattaa attttaaagg atcgtctgga attatattta
      721 attcaatttc tttaaaatta ttatctccaa ataatatttc tattcttttt tcagcagaca
      781 ttcgaaaatg ataattgcaa gtattgcata cccttaaatt ttcatttaaa tctttgtgat
      841 gcaacatatt tgcacaagaa gggcaattct gccataaatt ttcaggtacc tctctgcggt
      901 tcactagagc acttaatttt ggtttaacaa agtttgttag ccaattcatt ggttggttcc
      961 tttttttaat tcttttacaa aatcaccaat taactttaat attacatcag attggttaag
     1021 attattctca attattttta ctattgagga accaacaact gctccatcag taattgcaca
     1081 aatttctgaa acttgatttt tatcttttat accaaaacct gcgacaatag gaagattcga
     1141 ttgccttctg atttctttaa ctgaatgttt taactctttc atatcagcag atttttgacc
     1201 tgttatacct gtaatagtta cataatacaa aaatcctgaa gcatgttgaa taattttttt
     1261 aagtcttttg gggtcggtag tgggagttat gagccttatt agatcaatat catgaaattg
     1321 aatttcatcg aataattcag gatcttcttc aggttgcaaa tcaacaataa taaggccgtc
     1381 tatcccaatc tttgaacatt cacaaacaaa tctttttaca ccaaagtgga aaattacatt
     1441 ataatatccc ataagcacta ttggtatggc attattgatt tttctaatat ttttacaaat
     1501 gttaaatata gtttctaggt ttgttccgtt attaatagct ctattacttg ataattgaat
     1561 tgttggtcca tctgccattg gatcagaaaa aggcatccca atttcaacta catcagaacc
     1621 acattcaaca attttttcaa gaattttttg ggaagttttt ttgtccggat ctccacctgt
     1681 tacaaaagtt atcagttttt ttttcttgtc tgaaaaaact tttttaattc gttcagacat
     1741 tataatttaa tatttaattc tttagcaaca gtaaaaatat ccttatctcc tctgccacac
     1801 atattcataa ctactatttc gtccgaattc attttgggag caatttttat taatgcagct
     1861 aaagcatgag caggttcaag agcaggaata atgccctctt tgtgacaaca atattgaaat
     1921 gcttttaaag cttcttcatc tgttgcagaa acgtattgaa ctcttttttc atcaaataaa
     1981 aatgaatgct caggacctat accgggataa tccaaacctg ctgatatcga gtgtgcttct
     2041 tcaatttgtc cactatcaga ttgaagtaaa taggttttgt tgccatgcaa aacgcctggt
     2101 tgaccacccg ataaactggc agcatgtttt cctgacttta aacccaagcc agcagcttcc
     2161 acagctagca ttttaacctc attatcatca agaaaatcgt aaaataaacc taaagcattg
     2221 gaacccccac caatacaagc aacgagcaaa tctggtaatt tgttttcata ttcta
//
LOCUS       DTVV01000507            1618 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_542180, whole genome shotgun sequence.
ACCESSION   DTVV01000507 DTVV01000000
VERSION     DTVV01000507.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_542180"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="EYO86_06030"
     CDS             <1..235
                     /locus_tag="EYO86_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42624.1"
                     /translation="MGWDDLNWLEDVHMGYESGKPAVFDRNVNGWVTTPKNMKLPKDQ
                     QDRDMIARELLIKFQMSPKHPLVQLKKAYKKFD"
     gene            complement(232..777)
                     /locus_tag="EYO86_06035"
     CDS             complement(232..777)
                     /locus_tag="EYO86_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42625.1"
                     /translation="MSRTGWSNFVTWQCSLRQRNFRMFGGKPSEGTIASIYDQKTNEE
                     IADLRSILIEKKCLNTAKMFEFMIKKTHDPEDRFLKAVKFFSSEYYNSPVNFDGSFTA
                     TFTKQSEIVKKLLKKKKCNVQFFERETGFQFKVLINKLKKNDAEWKYTFFHNSFFNPS
                     LSENIEILCFNPVLNGLKKIN"
     gene            complement(797..>1618)
                     /locus_tag="EYO86_06040"
     CDS             complement(797..>1618)
                     /locus_tag="EYO86_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HIC42626.1"
                     /translation="EDANGQFEMNWNFSDCLQTADRHVFFKFMVKTLAEKKGLRATFM
                     PKPFLNKTGNGCHAHLSIWDKKGKNLFANKNDKLGLSKTAYNFMGGIMHSAEALTAWF
                     NPTINSYKRINAPVTASGATWSPNTITYSGNNRTHMIRVPGPGRFELRLMDGATNPYL
                     LQAGIIAAGLDGMNRKRNPGKPVHVNMYEESHKVKGAKKLPLTLEKSLDHLSKSKVMQ
                     SAFGKNIINSYVKLKNKEIQKYNSATEKKIGFRGAKKKYKGNIITQWEKDNTLDC"
BASE COUNT          497 a          277 c          254 g          590 t
ORIGIN      
        1 catgggctgg gacgatctta attggttaga agatgttcat atgggctatg aaagtggaaa
       61 gccagctgtt tttgacagaa atgttaacgg ctgggtaaca actccaaaga atatgaaatt
      121 accaaaggat caacaagaca gagacatgat agcaagagag cttttaataa aatttcaaat
      181 gtctccaaaa catccactag tgcaacttaa aaaagcctac aaaaaatttg attagttgat
      241 tttttttaac ccattcaaaa ccgggttaaa acataatatt tctatatttt cacttaaact
      301 aggattgaaa aaactattat ggaaaaatgt gtatttccat tctgcatcat tttttttgag
      361 cttattgatt aataccttaa attgaaaacc ggtttctctt tcaaaaaatt gcacattgca
      421 ttttttcttc tttaataatt ttttcacaat ttcggattgt ttagtaaatg tagcagtgaa
      481 tgagccgtca aaattaaccg gggaattgta gtattcagaa gaaaagaatt taactgcttt
      541 taaaaaacga tcttctggat catgagtttt tttaatcata aattcaaaca tttttgctgt
      601 gttaaggcat tttttttcaa ttaatatcga tcttaaatca gcaatttctt catttgtttt
      661 ttgatcataa attgaggcaa tagttccctc agagggtttg cctccaaaca tcctaaagtt
      721 tctttgccga agactgcatt gccaagttac aaaattactc caacctgttc gactcatatt
      781 aattatataa ttatgattaa cagtctaggg tattatcttt ttcccattgg gtaataatgt
      841 tacccttata ttttttcttc gcccccctaa aaccaatctt tttttctgta gcagaattgt
      901 atttttgtat ttccttattt tttaacttta catatgagtt aataatattt ttgccaaaag
      961 cagattgcat aactttactt ttacttagat ggtcaagaga tttttctaag gttaaaggta
     1021 atttttttgc tcccttaact ttgtgacttt cttcatacat attaacatga acaggttttc
     1081 ctgggttgcg ttttctattc attccatcca gtcctgctgc tatgataccg gcttgaagaa
     1141 gatatggatt ggtagcacca tccattaacc tcaattcaaa tctgccaggt ccaggcactc
     1201 ttatcatatg agtgcgatta tttcctgaat aagtaattgt gtttggagac caagttgccc
     1261 cagatgctgt gacaggggca ttaattctct tgtagctatt aattgttgga ttaaaccagg
     1321 cagttaaagc ttcagcagaa tgcattatgc cccccataaa gttgtatgct gtttttgata
     1381 aacctagttt atcattttta ttggcaaata agtttttacc ttttttgtcc caaattgata
     1441 aatgcgcatg acagccattt cctgttttat ttaaaaaagg tttgggcata aatgttgccc
     1501 gaagaccttt tttctcagca agagttttta ccataaattt aaaaaaaaca tgtctgtctg
     1561 ccgtttgcag gcaatcagaa aaattccaat tcatttcaaa ttgaccgtta gcatcttc
//
LOCUS       DTVV01000508            4871 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_552021, whole genome shotgun sequence.
ACCESSION   DTVV01000508 DTVV01000000
VERSION     DTVV01000508.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_552021"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="EYO86_06045"
     CDS             complement(<1..872)
                     /locus_tag="EYO86_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIC42627.1"
                     /translation="MIKHVTTVDQSDRKVPYNLRQSGPTPVQMLISTRVRKSPYWHLS
                     MEAGCWRATVYNRIYHPRGYVKPEDGGAMVEYEAIKNHVTMWNVAVERQIQVIGPDAE
                     AFVDYVITRDATKISPMRARYIILCNHMGGVLNDPILLRLSKDEFWFSLSDSDVSFYL
                     QGVNHDKRFNVEIDEIDVAPVQIQGPKSLALMKDLIGDQVDLDNMPFYGLAEAKVGSR
                     DCVISQSGFSGEAGYEIYLRDATLYADDMWNAVLEAGKKHSLMVIAPAHHRRIQAGIL
                     SWGQDMDNQHNPFQ"
     gene            1048..1953
                     /locus_tag="EYO86_06050"
     CDS             1048..1953
                     /locus_tag="EYO86_06050"
                     /inference="COORDINATES: protein motif:HMM:PF00691.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIC42628.1"
                     /translation="MKIILPLSLLFLFSCSASYEKLSKESFNPPTSLSKHLMEAYKTK
                     ADFEAQEMHDWNSAKLYAEKALGAADGKDILPEKISYWVIPADKQSELNKAYNNLMTV
                     YKEALLLDPFNLAKAISSLDCWAEQQEENWQTWDIDQCRNDFLSAMHEIFGSLEKEEE
                     ISTKQKINNEISKLDDSVSDDSVSIVTKNSENKILQIIYFDFDKSKLSDVSIEEIKNF
                     INANKKTIKKFIVVGHTDTMGTKKYNKVLSLERAEVVQKVLTELGIEKNNIHILGKGE
                     DNLRVQTADEVKHPANRRAEISPLN"
     gene            1999..2394
                     /gene="apaG"
                     /locus_tag="EYO86_06055"
     CDS             1999..2394
                     /gene="apaG"
                     /locus_tag="EYO86_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIC42629.1"
                     /translation="MYTKTTKKINITVNPYFLEDQSEPENQHYVWAYQVTINNLGKES
                     VKLKNRYWKIIDSNGTKQEVKGEGVVGEQPTLNPGEKFEYTSGTPLSTPSGFMEGYYE
                     METKNGSRFEASIPQFSLDSPFIDSNKLN"
     gene            2473..3861
                     /locus_tag="EYO86_06060"
     CDS             2473..3861
                     /locus_tag="EYO86_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIC42630.1"
                     /translation="MLIPMMVDLLYQKPDWEKFFFSSIITFFIGLVLYFSFKKEKILI
                     KVREAFVLTIFSWIIIAVFASLPFVFSSSNLNYTDAFFESISGITTTGATVINNLDDL
                     NEGILIWRSLLQWFGGIGIIVLAMAILPTLQIGGMQLLHMEQDDPYEKTLPKINHFII
                     EIFSLYLGLTIVCALLYYFFGMSGFDAILHSMTTISTGGFSAHNSSFGFFDSSSIETI
                     SIIFMIVGSLPFVVYLKFMHGERSSIIKDDQIKLFTLIIILLIGLTTLWLQKNSLHED
                     FLHSLRLSAFNITSILTGTGYTSTNYNTWGSFGLVIMIVIMFIGGCAGSTTGGIKIFR
                     LQLLFRGTITQIKKLTQPHGVFLTSFNGRSVTDDTFNSIMGFFFMYILIFIFASIALS
                     FFNIDFLTSFSAAASAISNVGPGLGTTIGPASNYSLLPDGAKWILSLTMLVGRLELFT
                     FLVLLSVGFWKK"
     gene            complement(3864..4451)
                     /locus_tag="EYO86_06065"
     CDS             complement(3864..4451)
                     /locus_tag="EYO86_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42631.1"
                     /translation="MLIYNIFLRYFILLAFLLVVFSCNKIEVLDQIVFDYNQLPKIVL
                     SAEQKKITETYEAKFSDPFIDHSLIKPPKEHLKSWIDSNLAIIGTENKLIINIIDSSL
                     TKSEVENANAKKYEEKTIYLFEINYLAEYILYDDSNSILSSTTVETKRSITSGKFISL
                     MESDRIIESLILDALTDFAKKTEELIKIHMSGYIL"
     gene            complement(4458..>4871)
                     /locus_tag="EYO86_06070"
     CDS             complement(4458..>4871)
                     /locus_tag="EYO86_06070"
                     /inference="COORDINATES: protein motif:HMM:PF00185.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIC42632.1"
                     /translation="SLLQAAEIFNFKLKIAVPESILISNHKMIKKMNLRYSKFFTQIS
                     KAVKDTNCVMTDVWVSMGEKNSKSKKIILQDFQVNDQVMQYAKKNAIFMHCLPAHRNE
                     EVTDSVIDGKQSKVWQQAQNRMYVQQSILNFLLKS"
BASE COUNT         1620 a          768 c          768 g         1715 t
ORIGIN      
        1 cactgaaaag gattgtgctg attatccata tcctgacccc atgacaatat tcctgcttga
       61 attcgcctgt ggtgagcggg tgcaataacc attaaactat gttttttccc cgcttccaaa
      121 acagcattcc acatatcatc agcataaagt gttgcatctc gtaaataaat ttcataccca
      181 gcttctccag aaaaacctga ttgcgatatt acacaatctc tactacccac tttggcttct
      241 gccaatccat agaaaggcat attatccaga tcaacttgat caccgatcaa atccttcatc
      301 aaagctaatg atttcggacc ttgaatttga acaggtgcta catcaatttc atcaatttca
      361 acattaaatc tcttatcatg atttactcct tgtaaataaa aactaacatc agagtctgaa
      421 agtgaaaacc aaaattcatc tttagaaagc cttaaaagaa tagggtcgtt tagcactcct
      481 cccatgtgat tacataatat gatataccgt gccctcattg gggatatttt agttgcatca
      541 cgtgtaatga catagtccac aaaagcttct gcatctggac ctattacttg tatttgcctt
      601 tcaacagcga cgttccacat agtaacgtga tttttaatcg cctcgtattc aaccattgca
      661 ccgccatctt caggcttaac ataaccacga ggatgataaa ttcggttata aactgttgct
      721 ctccagcatc cagcttccat agataagtgc caataaggtg attttctaac acgtgtagaa
      781 atcaacattt gcacaggagt tggcccactt tgacgaaggt tgtatggaac cttcctatca
      841 gattggtcaa ctgttgttac atgcttgatc atgcttaact cctcaataat aattattatc
      901 atgacgcggt atctaaagtt ctttttgaac gcaataaaaa acttggcatt tatcaaaata
      961 ttttggggat tcatcaatct taaatctgac aatcttgggc catatttttg acaataaaat
     1021 ggaagataga gaattgtgta tttgctaatg aagattattc tcccccttag ccttttattt
     1081 cttttttcat gttccgcaag ttatgaaaag ctgagcaaag aatcatttaa tccccctaca
     1141 tctttatcta agcatttaat ggaagcttat aaaacaaaag ctgactttga agcacaagaa
     1201 atgcatgatt ggaactcagc taaattatat gctgaaaaag ctcttggtgc ggctgatgga
     1261 aaagatatat tgcctgaaaa aattagttat tgggttattc ctgctgataa acaatctgaa
     1321 ttaaataagg cgtacaataa tttaatgaca gtctataaag aagcgttact tctagatcct
     1381 tttaacttag caaaagctat tagttcactc gactgctggg ctgaacagca agaagaaaat
     1441 tggcaaacat gggatatcga tcaatgtcgc aatgattttc tatccgctat gcatgagatt
     1501 tttggcagtt tagaaaaaga agaagaaatt tcaactaaac aaaaaatcaa taatgagatt
     1561 tctaaattag atgatagtgt aagtgatgat agtgtaagta tagttactaa aaattcagaa
     1621 aataaaatat tgcaaataat atattttgat tttgataaat ccaagttatc agatgtgagt
     1681 atagaagaaa ttaagaattt tattaacgca aataaaaaaa ctattaaaaa atttattgta
     1741 gtaggccaca ctgatacaat gggaacaaag aaatacaata aggtgctatc tttagagaga
     1801 gcggaagtgg tacaaaaagt cttaacagaa cttggtattg aaaaaaataa tattcacata
     1861 cttgggaaag gtgaggataa tttgagagta caaactgcag atgaagtaaa acatccggca
     1921 aatagaaggg cggaaattag ccctttaaat tagcttctat tacttcgcat cttggtgtaa
     1981 tactcactca caattttcat gtacacaaaa acaactaaaa aaattaatat aacggtaaat
     2041 ccttattttt tagaagatca atctgagcct gaaaatcaac attatgtttg ggcctatcaa
     2101 gtaaccatca ataacttggg caaagaaagt gtaaagctga aaaatcgata ttggaaaata
     2161 attgactcaa atggcacaaa acaagaagtt aaaggagaag gtgtagttgg ggaacagcca
     2221 acacttaatc ctggagaaaa atttgaatat acaagtggaa cacctttatc aactccttca
     2281 ggttttatgg aaggttatta tgagatggaa acaaaaaatg gatctagatt tgaagcttca
     2341 atacctcaat tttccttaga tagccctttt attgattcta acaaattaaa ttaataatag
     2401 ccaattttaa tgcgtgattt ccgttctgta ttaaatatta ttggtttgct tttgtgcatt
     2461 gaggcgttag ctatgctaat tccaatgatg gttgatttat tataccaaaa acctgattgg
     2521 gaaaaatttt ttttttctag tataataact ttttttattg gcttagtttt atatttttct
     2581 tttaaaaaag aaaaaattct tattaaagtt cgagaagctt tcgtattaac gattttttct
     2641 tggattatta tagctgtttt tgcttcttta ccttttgttt tttcttcttc aaatctaaat
     2701 tatacagatg ctttttttga gtccattagt ggaataacaa caactggcgc cactgttatt
     2761 aataatttag atgacttaaa tgaaggtata ttaatttggc ggtctttgct tcagtggttt
     2821 ggtggcatag gaattattgt attagcaatg gcaattcttc caacactaca aattggaggg
     2881 atgcaacttt tgcatatgga acaagatgat ccatacgaga aaactctacc taaaattaat
     2941 cattttataa ttgagatttt ctctctgtat ctaggcttaa caattgtttg tgctctacta
     3001 tactattttt ttggtatgag tgggtttgat gctattttac attcaatgac tactatatct
     3061 acaggtggtt tttcagcaca caactcctct tttggatttt ttgattcatc atcaattgag
     3121 acaataagta ttatattcat gattgttgga agtttgccat ttgtagtcta tttaaaattc
     3181 atgcacggtg agcgatcatc tataattaaa gatgatcaaa ttaaattatt taccttaatt
     3241 attattttgc taattggttt aacaactcta tggttgcaaa aaaattcatt gcatgaagac
     3301 tttcttcatt cacttagatt atcagccttt aacatcacat ctatcttaac aggaactggt
     3361 tatacaagta ctaattataa tacatgggga agttttggtt tggtaattat gatcgttata
     3421 atgtttattg gaggttgtgc cggatccaca acaggaggca taaaaatatt tcgtcttcaa
     3481 ttattatttc gaggtacaat aactcaaata aaaaaattaa cacaacctca tggtgttttc
     3541 ctaacttcgt tcaacggaag atctgttacg gatgatactt ttaattccat aatgggattt
     3601 ttctttatgt atattcttat ttttattttt gcttcaattg cacttagttt ttttaatata
     3661 gattttctaa catctttttc agctgctgct tctgctattt caaatgttgg tccaggatta
     3721 ggaacaacaa ttggtcctgc atcaaattat agtttattgc cagatggagc gaaatggata
     3781 ctatcattaa caatgctagt tgggaggttg gaactgttta cttttttagt attattgtct
     3841 gttggttttt ggaaaaaata aattcataaa atatatcctg acatgtgtat ctttatgagt
     3901 tcttcagttt ttttagcaaa atcagttaaa gcgtctaaaa taagactctc aattatcctg
     3961 tctgactcca tcaaagaaat aaattttcct gaagtgatag aacgcttagt ttcaactgtt
     4021 gtggacgata gtattgaatt tgaatcatca tacaggatat attcagctaa ataattaatt
     4081 tcaaataaat atattgtctt ttcttcatat tttttagcgt tagcattttc cacttctgat
     4141 tttgttaacg atgaatcaat aatattaata attaatttgt tttcagttcc aataatagca
     4201 agattactgt ctatccaaga ttttaagtgt tcttttggag gttttatcaa agaatgatcg
     4261 ataaacggat cgctaaattt tgcttcatat gtttctgtta tttttttttg ttcagcgctc
     4321 aaaacaattt ttggaagttg attgtagtca aaaacgattt ggtctaaaac ttcaatttta
     4381 ttgcatgaaa aaacaaccaa taaaaaagct agtaaaataa agtatcgtaa aaaaatatta
     4441 tatattagca tacaacacta actcttaagc aaaaaattaa gaatactctg ctgaacatac
     4501 attctgttct gagcttgttg ccatactttt gactgcttcc catcaatgac tgaatcagtt
     4561 acttcttcat ttcgatgagc tggtaaacaa tgcataaata tagcattttt ttttgcgtat
     4621 tgcataactt ggtcattaac ttgaaaatct tgtaaaatta ttttttttga tttagagttt
     4681 ttttctccca tcgaaaccca gacatctgtc ataacacaat tagtatcttt aactgctttt
     4741 gaaatttgtg taaaaaattt tgaatatcgt aaattcattt ttttaatcat tttatgattt
     4801 gatattaata ttgactctgg aactgcaatc tttagtttaa aattaaaaat ttctgctgct
     4861 tgaagtaatg a
//
LOCUS       DTVV01000509            1215 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_555255, whole genome shotgun sequence.
ACCESSION   DTVV01000509 DTVV01000000
VERSION     DTVV01000509.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_555255"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="EYO86_06075"
     CDS             complement(<1..507)
                     /locus_tag="EYO86_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bb3-type cytochrome oxidase subunit IV"
                     /protein_id="HIC42633.1"
                     /translation="MEESASTVEVTIPPNKGLATEWASDQKAFKGVPWGKAMMWIFLV
                     SDTFVFSIFLISYMTVRFSTKAEWPNPSEVFALHVGHVSVPLLLIAIMTFILITSSGT
                     MALAVKYGYEKNRKMCAILMFATAIFGASFVGMQAFEWTKLIMEGVRPWANPFGAPEF
                     GSIFFMVTG"
     gene            complement(521..1156)
                     /locus_tag="EYO86_06080"
     CDS             complement(521..1156)
                     /locus_tag="EYO86_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative cytochrome c oxidase polypeptide
                     CoxN"
                     /protein_id="HIC42634.1"
                     /translation="MRKKESIFQILAQKPWDPSQAKIDDMHEGGTINLSKGKLGLRFI
                     MFVSTIFFCLFIVTYSDRMVYPDWQRMPEPWLLWINTAVLFFSSMVFVSTQIASKNNQ
                     FQLVKNRLLLIGFLAFAFLVGQLLVWQQLIANGFYVSTNPSNAYFYVFTALHGLHLLG
                     GLVYWYLTIKKVWISSNIVISKAKHTVDLCAIYWHFLLAVWVVLFGLMLFS"
BASE COUNT          437 a          252 c          179 g          347 t
ORIGIN      
        1 acccgtcacc ataaaaaata ttgatccaaa ttcaggagct ccaaaaggat ttgcccatgg
       61 acgcacacct tccatgatta attttgtcca ttcaaaagcc tgcataccaa caaaggacgc
      121 tccaaaaata gcagttgcaa acatcaaaat ggcacacatc tttcgatttt tctcgtatcc
      181 atattttact gccaaggcca ttgtgccact gcttgttatt agaatgaaag tcattattgc
      241 tattaacaaa agtgggacac tgacatgacc aacatgcaaa gcaaaaactt cacttggatt
      301 cggccattcc gcttttgtag aaaaccgaac agtcatgtat gaaattaaaa aaatactgaa
      361 tacaaacgta tcactaacta gaaaaatcca catcatggct tttccccaag gaactccttt
      421 aaaagctttt tgatcagatg cccattcagt tgcaagacct ttgtttggcg gaattgtaac
      481 ttcaactgtt gacgcagatt cttccattaa ttaatcccca ttatgaaaat aacattaaac
      541 caaataaaac aacccacaca gccaataaaa aatgccaata aatagcacat aaatcaaccg
      601 tatgtttagc tttagaaata actatattgc ttgaaatcca tactttctta attgttaaat
      661 accaataaac aagaccaccc aacaaatgaa ggccgtgaag tgctgtgaat acataaaaat
      721 atgcattgga tggattagtg gatacataaa atccatttgc gattaattgc tgccaaacca
      781 ataattggcc aacaagaaat gcaaaagcta aaaatccaat taataaaagt ctgttcttga
      841 ctaattggaa ttgattattc tttgatgcaa tttgagtgct gacaaaaacc atgctactaa
      901 aaaacaatac tgcagtgttg atccataaca gccaaggctc cggcattctt tgccaatcag
      961 ggtaaaccat tctatcagaa taagtgacaa taaataaaca aaagaaaatt gtactgacaa
     1021 acataataaa cctaagtcca agcttgcctt tacttaaatt tattgttcct ccttcatgca
     1081 tatcatcaat tttagcttga gatggatccc aaggcttttg agctaaaatt tgaaaaatac
     1141 tttctttttt tctcatgtct ttatcgcttc tgcaccaggt acttcgcttg gaggaacatt
     1201 ctgcggaatg aagtc
//
LOCUS       DTVV01000510            1080 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_606643, whole genome shotgun sequence.
ACCESSION   DTVV01000510 DTVV01000000
VERSION     DTVV01000510.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_606643"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="EYO86_06085"
     CDS             <1..381
                     /locus_tag="EYO86_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIC42635.1"
                     /translation="YDGVVFHRVIDSFMAQTGDVQFGNSNSESYDLKRVGTGGSKYPD
                     LKSEFSELPHERGTLSMARSADPNSANSQFFICFKSAPNLDRQYTVFGKVVEGMEFVD
                     LIKKGEGSNGEVSEPDKIISLLAK"
     gene            396..>1080
                     /locus_tag="EYO86_06090"
     CDS             396..>1080
                     /locus_tag="EYO86_06090"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HIC42636.1"
                     /translation="MSKENFSFNLLNEDNFSRLGQISTHRGNIDTPVFMPVGTQGTIK
                     SAFIEDVVLAGVQIILSNTYHLMIRPGTEIIKRAGGIHEFMNCNLPILTDSGGFQIMS
                     LSKLVKIDKIKGAIFNSHLDGKKFTLSPEESIRIQKDLNSDIVMVLDECPKLSINKNK
                     ISQSLKLSHNWAERSKNEFGNNPKKALFGIVQGGIYKDLRLESLDGLVKLDFDGYALG
                     GLAVGETQKQ"
BASE COUNT          376 a          137 c          202 g          365 t
ORIGIN      
        1 tatgatggtg tagtttttca tagagttata gatagtttta tggctcagac gggtgatgtt
       61 caatttggaa attccaactc agaaagttat gatcttaaac gtgtaggaac tggtggatca
      121 aaatatcctg atttaaaatc tgaatttagt gaattacctc atgaaagagg aactttatct
      181 atggctcgtt ctgctgaccc aaatagtgct aatagtcagt tttttatttg ttttaaatca
      241 gctcctaatt tagataggca atacactgtt tttggaaaag tagttgaagg aatggaattc
      301 gttgatttaa ttaaaaaagg agagggctct aacggagagg tttcagagcc agataaaatt
      361 ataagtctac ttgccaaata aaatattgcc tcttcatgtc taaagaaaac ttttctttca
      421 atcttttgaa cgaagataac ttttctcgcc ttggtcaaat ttctacacat cgtggaaaca
      481 tagatactcc ggtttttatg cctgttggca cacaaggtac aattaaatct gcttttattg
      541 aagatgtagt attagctggt gttcaaataa ttttatctaa tacatatcat ttaatgatta
      601 gaccaggaac tgaaataatt aaaagagccg gaggaataca tgaatttatg aattgtaatc
      661 tacctatatt aactgactct ggcggttttc aaattatgtc gttatctaag cttgttaaaa
      721 ttgataaaat aaaaggagcg atttttaatt cacatttaga tggtaaaaaa tttactttaa
      781 gtcctgaaga aagtattaga attcaaaaag atttaaattc tgatattgtg atggttttag
      841 atgaatgtcc aaaattgtct attaataaga ataaaattag tcaatcttta aaactttctc
      901 acaactgggc agaaagatca aaaaatgaat ttggaaacaa tccaaaaaaa gctttatttg
      961 gaatcgttca aggtggcatc tataaagatt taagattaga gagtttggat ggattagtaa
     1021 aacttgattt tgatggatat gcattggggg ggttggctgt tggtgaaacc caaaaacaaa
//
LOCUS       DTVV01000511            1993 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_608058, whole genome shotgun sequence.
ACCESSION   DTVV01000511 DTVV01000000
VERSION     DTVV01000511.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_608058"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            67..1599
                     /locus_tag="EYO86_06095"
     CDS             67..1599
                     /locus_tag="EYO86_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIC42637.1"
                     /translation="MEDIIKKLEEKRKKAQEGGGKARIEAQHKKGKLTASERIDLLLD
                     KGSFEEWDMFVEHRSSDFGMDKNKIPGDGVITGHGTINGRLVFVFSQDFTVFGGSLSE
                     AHADKIVKIMDKALKVGAPVIGLNDSGGARIQEGVDSLAAYAKVFEKNVLASGVIPQI
                     SVIMGPCAGGAVYSPAMTDFIFMVNKTSYMFVTGPDVVKTVTHEDVTYEELGGASTHT
                     TKSSVADGAFKNDIIALKEIRRLFDFFPLSFREKPPKKKTKDNPDRIEKSLNTLVPKN
                     PNKPYDMKELIYKIIDDGDFFEIQENYAKNILIGFARMDGYPVGIVANQPLVLAGCLD
                     IDSSRKAARFVRFCDCFNIPIITFVDVPGFLPGTKQEYGGIIKHGSKLLFAYTEATVP
                     KITIITRKAYGGAYDVMSSKHLRGDVNYAWPNAEIAVMGPKGAVEIIFRDDMKDKKKI
                     EERTEEYRKKFANPFIAASRGFLDDIIRPAGTRPRLCKALKMLKNKFLKNPPKKHNNI
                     PL"
     gene            1605..1886
                     /locus_tag="EYO86_06100"
     CDS             1605..1886
                     /locus_tag="EYO86_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42638.1"
                     /translation="MFKKILIANRGEIACRVIKTAKKMGIKTVAVYSEADEYSKHVIE
                     ADESVFIGNAVASESYLSIDKIINAAKKTKTDAIHPGYGFLSELLSIPI"
BASE COUNT          736 a          278 c          381 g          598 t
ORIGIN      
        1 aaaaaaaatt agtagaatta tttaaaatta tacttaagta acgtctttgt ttttttaaat
       61 tttcatatgg aagatataat caaaaaattg gaagaaaaaa gaaagaaagc tcaagaaggc
      121 gggggcaaag cgcgcataga agcacaacac aaaaaaggta aattaacagc cagtgaaaga
      181 atagatctgt tgctggataa agggtcattt gaagaatggg atatgtttgt ggaacataga
      241 tcttcagatt ttggtatgga taaaaacaaa atacctggcg atggtgtcat taccggacat
      301 ggaactatca atggaagatt ggtttttgtt ttcagtcagg attttactgt ttttggtgga
      361 tccttatctg aggctcacgc agataagatt gtcaaaatta tggataaagc acttaaagtt
      421 ggagcgccag ttattggttt aaatgattca ggcggagcaa gaatacaaga aggcgttgat
      481 tcattggcag catatgcaaa agtttttgag aaaaatgttt tggcttctgg agttattcca
      541 cagatatctg taattatggg gccatgcgct ggaggggctg tttattcacc ggctatgaca
      601 gattttattt ttatggtaaa caaaacttca tatatgtttg tgacaggacc agatgttgta
      661 aaaactgtta cacatgagga tgttacatat gaagaattag gaggagcatc aacacataca
      721 acaaaatcga gcgttgctga cggagccttt aaaaatgata ttatagctct taaagaaatt
      781 agaagattat ttgatttttt tcctttatca tttagggaaa aacctccaaa aaaaaagacc
      841 aaagacaacc ccgatagaat tgaaaagtct cttaatacat tggttccaaa aaatcctaat
      901 aaaccttatg atatgaaaga attaatatac aaaataattg atgacggaga cttttttgaa
      961 atccaagaaa attatgcaaa aaatatactt ataggctttg caagaatgga tggatatccg
     1021 gtaggtatag ttgctaatca accattagtt ttagcaggat gtttagatat tgactcttct
     1081 agaaaagctg caaggtttgt aagattttgt gactgtttta atatcccaat aattactttt
     1141 gttgatgttc caggattttt accaggcacc aaacaagaat atggaggaat tattaagcac
     1201 ggttcaaaat tattgtttgc atatacagaa gcaactgttc ctaaaattac aattatcaca
     1261 cgcaaagcat atggaggagc atatgacgtg atgagttcaa aacatctacg aggtgatgta
     1321 aattatgctt ggccaaatgc agaaattgca gtaatgggtc ccaagggagc tgttgaaata
     1381 atttttcgtg acgatatgaa agataaaaag aaaattgaag aaagaactga agaatataga
     1441 aaaaagtttg caaatccttt tattgcagca tccagaggtt ttctcgatga tataataaga
     1501 cctgcaggaa cgagacctag actttgtaaa gcgttaaaaa tgttaaaaaa taagtttttg
     1561 aagaatcctc ccaagaaaca caacaacatt cctctttaaa aaaaatgttt aaaaaaattc
     1621 tcattgcaaa tagaggagaa attgcatgta gagtaataaa aactgcaaag aaaatgggaa
     1681 ttaaaactgt agcagtttat tctgaagctg atgaatattc taaacatgtt attgaagccg
     1741 atgaatctgt ttttattgga aatgctgtag catcagaaag ctatctctca attgataaaa
     1801 taattaatgc cgcaaaaaaa accaaaacag atgcgattca tcctggttat ggttttttat
     1861 ctgaacttct ttctataccc atttgatcaa tcatatttat tattttatct aatgaaccag
     1921 gcgatgcaac ttgaccacag tgtcgagaac tttgacaatc taaacatttt actacataat
     1981 cattaagtgc ttg
//
LOCUS       DTVV01000512            1126 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_612414, whole genome shotgun sequence.
ACCESSION   DTVV01000512 DTVV01000000
VERSION     DTVV01000512.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_612414"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="EYO86_06105"
     CDS             <1..526
                     /locus_tag="EYO86_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HIC42639.1"
                     /translation="KFSEIAALFPEGYITLLEMYLDTVNNQMTEDSKKINLIRLRVHE
                     RVKELVILRLKIMSREKELISKTYFHLLLPQNFKIASRCLYKAVDQIWFVAGDNSTDF
                     SFYSKRAILASIYSMVMLHYVNNNSLDQTIELLNKQLKRVSKIPKIKNRLGDITKTIS
                     QIFKLVKNIRPIKQ"
     gene            complement(516..716)
                     /locus_tag="EYO86_06110"
     CDS             complement(516..716)
                     /locus_tag="EYO86_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HIC42640.1"
                     /translation="MGRRELLIKQLKEFQEEHRDLDAILIQLQEKQTIDFVQIKRLKK
                     RKLLLKDKIRSLKNNIEPDIIA"
     gene            784..>1126
                     /locus_tag="EYO86_06115"
     CDS             784..>1126
                     /locus_tag="EYO86_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIC42641.1"
                     /translation="MVKKIAILTSGGDCAGLNAVIRAATIRAVNKYKWQIFGIKDGTL
                     GLMTDPLNVVELKPSNFDGNLMRMGGTFLGTNSKGYPSHEEVIKGFKKMELDALIGIG
                     GDGSMKILKTLT"
BASE COUNT          385 a          152 c          195 g          394 t
ORIGIN      
        1 taaattttca gaaatagccg ccttatttcc tgaagggtac attacccttc ttgaaatgta
       61 tttggataca gtcaacaatc aaatgacaga ggattcaaaa aaaattaatc ttattcgttt
      121 aagagttcat gaacgtgtta aagagttagt tattttacga ttgaagatta tgtcacgaga
      181 gaaagagtta atttcaaaaa cctattttca tttactgctt cctcaaaatt ttaagatagc
      241 ttcaagatgt ctttataaag ctgttgatca gatatggttt gtggcgggag ataattctac
      301 agattttagt ttttattcaa aaagagcaat tttagcctca atttatagca tggttatgtt
      361 gcattatgtt aacaataata gtttagatca aaccatcgaa ttgttaaaca aacaattaaa
      421 aagagtctct aagataccaa aaataaaaaa cagattggga gatataacga aaacaatttc
      481 tcagattttt aaattagtta aaaatattcg tcccatcaag caataatatc aggttcgata
      541 ttattcttga gactacgaat cttatcttta agaagtaact ttcttttttt taatctttta
      601 atttgtacaa aatcaatagt ttgcttttct tgcaattgaa tgagaattgc atcaagatca
      661 cgatgttcct cttgaaattc tttgagttgt ttgatgagta actcacgtct tcccattttt
      721 tgatttgtag tatttaattt attaaatatt ttttcttttt cagtattagt atacaagacc
      781 ccaatggtaa aaaaaatagc tattttaaca agtggaggcg attgcgcagg tttgaacgca
      841 gtaattcgag ctgctacaat tcgggcagtt aacaaatata aatggcaaat ttttggaatc
      901 aaagatggaa cattgggctt gatgacagat cctcttaatg tggttgagtt gaaaccttcc
      961 aattttgatg gaaatttgat gcgtatgggc ggcacttttt taggcactaa tagcaaaggc
     1021 tatccttcac atgaagaggt aattaagggt tttaaaaaaa tggaattaga tgctttaatc
     1081 ggaattggtg gtgatggaag tatgaaaatt cttaagacct tgacaa
//
LOCUS       DTVV01000513            1286 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_647, whole genome shotgun sequence.
ACCESSION   DTVV01000513 DTVV01000000
VERSION     DTVV01000513.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_647"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="EYO86_06120"
     CDS             complement(<1..1283)
                     /locus_tag="EYO86_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIC42642.1"
                     /translation="MPLLPLRDIVVFPGMITPLFVGRDKSIKALESVMDKDKKIFLVT
                     QKNPQDDSPSTNDIYNTGCVGKVVQLLKLPDGTVKVLIEATEKAEIVKYLKTDNFFVA
                     EVRIIQNKPQLVKSKHLSALTRRVLSRFEDYSKLNRTLPSDSSVSLNQIKDLEQLVNT
                     ISSYLVLKIDDKQKLLCISSVDKLFEKILMHLENEIGVLEVEKKIKSRVKRQMEKTQR
                     EYYLNEQLKAVQKELGDLENSQDEFSELENKIKTLQLTKEAKEKAESELKKLRSMSPM
                     SAESTVVRNYLDWILSVPWKNPTLINKKISNAERILNDDHYGLEKVKERILEYLSVQQ
                     RVNKIKGPILCFVGPPGVGKTSLGKSIAKATGRKFVRVSLGGIRDEAEIRGHRRTYIG
                     SMPGKIIQGMKKAGKSNPLFLLDEIDKMGYDFRGDP"
BASE COUNT          383 a          230 c          158 g          515 t
ORIGIN      
        1 gggtcacctc taaaatcgta tcccatctta tctatctcat ctaataaaaa aagagggttt
       61 gatttaccag cttttttcat accttgtata attttacctg gcatagatcc gatataggtt
      121 cttctatgac ctcgaatttc agcttcgtct ctaatacccc ccaaagaaac tcttacaaat
      181 tttctaccgg tagcttttgc aattgattta cctaatgacg tttttccaac tccaggagga
      241 ccaacaaaac ataaaatagg acctttaatt ttattcaccc tttgttgaac tgataaatat
      301 tccaatattc tttcttttac tttttctagt ccataatgat catcattaag aattctttct
      361 gcattactaa tttttttatt aattaaggtt ggatttttcc atggtactga caaaatccag
      421 tccaaataat ttcttacaac agtagattcg gcagacattg gactcatgct tcttaatttt
      481 tttaattctg attctgcttt ctctttagct tctttggtaa gttgtaatgt ttttatttta
      541 ttttctaact ctgaaaattc atcttggcta ttttctaagt cgcctaattc tttttgaaca
      601 gctttgagtt gttcatttaa ataatattcc ctttgtgttt tctccatttg tctttttaca
      661 cgagatttaa tcttcttttc aacctctaat actccaattt cgttttctaa atgcataagt
      721 atcttctcaa acaatttgtc tacactgcta atacataaca atttttgttt atcatctatt
      781 ttcaatacta aatatgaaga aattgtatta acaagttgtt ccaaatcttt aatttgatta
      841 agtgacactg aactatctga cggtaaagtg cgattaagtt tagaataatc ttcaaacctt
      901 gataatactc ttcttgttaa tgcgcttagg tgcttacttt taactaattg aggtttattt
      961 tgtataattc taacctcagc aacaaaaaaa ttatcagttt ttaaatattt tacaatttct
     1021 gctttttcag ttgcttctat taaaactttt actgttccat caggaagttt taataattga
     1081 acaactttgc caacacatcc tgtattatat atgtcgttag ttgaaggaga atcatcttgt
     1141 ggattttttt gtgtaacaag aaaaattttt ttatctttat ccataacaga ctctagtgct
     1201 ttgatagact tatcccttcc aacgaacaat ggagtaatca ttcctggaaa tacaacaata
     1261 tctcgcaaag gaagtaatgg aataga
//
LOCUS       DTVV01000514            2402 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_652972, whole genome shotgun sequence.
ACCESSION   DTVV01000514 DTVV01000000
VERSION     DTVV01000514.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_652972"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="EYO86_06125"
     CDS             <1..345
                     /locus_tag="EYO86_06125"
                     /inference="COORDINATES: protein motif:HMM:PF01464.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIC42643.1"
                     /translation="NKKVKKNNGNILKIPEINLDVGQEYIEYLLNLKSVNNNLIYLAA
                     AYNGGPGNLSKWKENTNYLDDPLFFMESIPSRETRWFIEKVLTKYWIYQDKNGIQSNS
                     LTMLANGENPIY"
     gene            352..882
                     /gene="moaB"
                     /locus_tag="EYO86_06130"
     CDS             352..882
                     /gene="moaB"
                     /locus_tag="EYO86_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HIC42644.1"
                     /translation="MTKIHNPSEFISINIALLTISDTRIIENDKSGNLLQERITSFGH
                     NLKGRNIIKDDFEIIKQTFVNLSNDPLIDAIISTGGTGLTGRDSTPEAVIELSDKMID
                     GFGELFRQISFNKIGTSTIQSRAIGAIINGTYVFCLPGSPGACKDGWDDILQYQLDIR
                     HKPCNFIEIMPRLKEK"
     gene            884..1510
                     /locus_tag="EYO86_06135"
     CDS             884..1510
                     /locus_tag="EYO86_06135"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010082329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIC42645.1"
                     /translation="MPDFNIEQYFNQNVIGIDEVGRGPLAGPIISCAFIFFDTSINPK
                     DLFFIDDSKKLTLKKRHLAIKKIIDLKKNKKINFKLGMASVEEIDQHNILEATKISMK
                     RAVKKLELSPTQLIIDGNIDLELNDFPSKGIIKGDQKSFSIAAASIIAKIYRDRYMQF
                     LSFLFPDYDWSNNAGYGTKKHIEQIYKKGITIHHRKSFQPIKSLIHSK"
     gene            1571..>2402
                     /locus_tag="EYO86_06140"
     CDS             1571..>2402
                     /locus_tag="EYO86_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HIC42646.1"
                     /translation="MIKKNSILHGDSLELLKQIPDKSVDLVFADPPYNLQLKDTLYRP
                     DQTAVEAVTNDWDKFDTYHAYDQFCLQWLNECKRVLRDGGALWVIGSYHNILRLGTSI
                     QNLGFWILNDIIWHKTNPMPNFRGTRFTNAHETLLWCTTARKAKYTFNYQNLKELNEG
                     KQMRSDWHIPICSGKERLRDDNHQRSHPTQKPEALLYRILLASSQKGDLILDPFLGSG
                     TTAVVAKKLQRNFIGIEQDKDYITLAQKRLNETQVLKDNLVTLTQSRKDLPKVPFGEL
                     V"
BASE COUNT          875 a          395 c          395 g          737 t
ORIGIN      
        1 aataaaaaag taaaaaaaaa taatgggaat attctaaaaa ttcctgaaat taatcttgat
       61 gttgggcaag aatatattga atatttatta aacctaaaat cagttaataa taatttaatt
      121 tatttagctg cggcttataa cggcggtcct ggaaatctca gtaaatggaa agaaaatact
      181 aactatcttg atgatcccct tttctttatg gaaagcattc cgtcaagaga aacaaggtgg
      241 tttatcgaaa aagttttaac taaatattgg atttatcaag ataagaatgg aattcaatca
      301 aattcattaa ctatgctagc caatggagaa aatcccattt attaatagct aatgacaaaa
      361 attcataacc cctctgaatt catctcaatt aacatagccc tccttaccat ttcggatact
      421 cggataattg aaaatgacaa atcaggaaat ttgttacaag aacgcataac ttcatttggt
      481 cataacctaa aagggcgcaa tataattaag gatgattttg aaataattaa acagactttt
      541 gttaatttat ctaacgaccc actgattgat gcaattattt ccactggagg aactgggcta
      601 acagggagag attcaacgcc agaagcagtg atagaattat cagataagat gattgatggt
      661 ttcggtgaat tatttcggca aataagtttt aacaaaattg gcacttccac tattcaatca
      721 cgggctattg gtgctattat taatggtaca tatgtatttt gtttgccagg atctcctgga
      781 gcttgcaaag acggatggga tgatattttg caatatcaat tagatattcg tcacaaacct
      841 tgtaacttca tcgaaatcat gccgcgccta aaagaaaaat aatttgcctg attttaatat
      901 tgaacaatat tttaaccaga atgtgattgg tattgatgaa gtcggaagag gacctttagc
      961 aggacctatc attagttgtg catttatttt ttttgacaca tcaataaatc caaaagattt
     1021 attttttatt gatgactcta aaaaattaac acttaaaaaa agacatttag caataaaaaa
     1081 aattatagat ttaaaaaaaa ataaaaaaat taattttaag ttaggaatgg caagtgtcga
     1141 agaaatcgat caacacaata tattagaggc aacaaaaata tcgatgaagc gtgcagtaaa
     1201 gaaacttgaa ctttccccca ctcaattgat tattgacgga aatattgatc tagaactaaa
     1261 tgattttcct agtaaaggca tcattaaggg tgatcaaaaa tcttttagta tagcggcagc
     1321 atctattatc gctaaaatat accgagatcg ctatatgcaa tttttatctt ttctatttcc
     1381 tgattatgat tggtcaaata atgcaggtta tggaactaag aaacatattg aacaaattta
     1441 taaaaaagga ataacgattc atcatcgaaa aagttttcag cctataaagt cacttatcca
     1501 tagtaaatga ttctgcctgt tgataacttc ggttgaccta ttattaaatc tccttaaaga
     1561 ttcttctttg atgattaaaa aaaattccat tcttcacggc gatagtcttg aactattaaa
     1621 acaaattcca gataaatcag ttgatcttgt gtttgcagat cctccatata atcttcaatt
     1681 aaaagacact ctctatcgcc ctgatcaaac agccgtggaa gctgtcacaa atgattggga
     1741 taaatttgat acttatcatg cctatgatca attttgtcta caatggctga acgaatgtaa
     1801 aagagtttta agggatggcg gtgctctgtg ggttatagga agttatcata atattctgcg
     1861 tctcggcaca tccatacaaa atttaggttt ttggatatta aatgacatta tttggcataa
     1921 aactaatccc atgcctaatt ttagaggcac acgatttact aacgcccatg aaacattact
     1981 ttggtgcact actgcgagaa aagccaaata cacttttaat tatcaaaatc taaaagagct
     2041 taatgaagga aagcaaatgc gaagtgactg gcacattcct atttgttcgg gaaaagaacg
     2101 tttgcgcgat gataatcatc agcgctccca ccctacgcaa aaaccagaag ccttgcttta
     2161 ccgcatcctt ttagcatcat ctcaaaaagg agatttaatt ttagatcctt ttctaggttc
     2221 aggaaccaca gcggttgtcg caaaaaaatt acaacggaac ttcataggta ttgagcaaga
     2281 taaagactac atcacccttg cccaaaaacg attaaatgaa acgcaagtct taaaagataa
     2341 tttagtaact ctcactcaaa gcagaaaaga tttaccaaaa gttccttttg gggaattagt
     2401 cg
//
LOCUS       DTVV01000515            3449 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_662502, whole genome shotgun sequence.
ACCESSION   DTVV01000515 DTVV01000000
VERSION     DTVV01000515.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_662502"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="EYO86_06145"
     CDS             complement(<1..420)
                     /locus_tag="EYO86_06145"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:PRK05426.3-1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIC42647.1"
                     /translation="MWLICGLGNPGKKYFLTRHNVGFDVVDSLIDKNNCELIKKDRKK
                     ELYKGTIDNNECLFCKPLTYMNISGQPIREIVNFFKIPKSKIIVIHDDLDLAVGKIKI
                     KIGGGNGGHNGLLSIDEAIGKNFKRLRIGIGHPGSNDL"
     gene            complement(457..1164)
                     /locus_tag="EYO86_06150"
     CDS             complement(457..1164)
                     /locus_tag="EYO86_06150"
                     /inference="COORDINATES: protein motif:HMM:PRK05618.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIC42648.1"
                     /translation="MENIKVIARSKDSGSTKSLLKKGMVPGIIYGKNSEPSQIVFEDK
                     ALQKLMHSGVFYSKIINLDVEGKKEKVLPKQLQYHPVTDKLIHFDFLRVQDDTKVTVE
                     IEVEFLNRDICPGLKQGGVLNLVRRVVELSCNANNIPEKLQYDLVSAEIGDAIKISNI
                     ELPDGVKPTITDRDFVVATLVPPTIEVEEEPTPEEEVEGEAIEGETEEGTDSKEDQDS
                     GDSSEDKKAEPAEDKPK"
     gene            complement(1181..2116)
                     /locus_tag="EYO86_06155"
     CDS             complement(1181..2116)
                     /locus_tag="EYO86_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIC42649.1"
                     /translation="MKIISCNSNTMLAEAIASYIGVNLSDATIKKFADGEVFVKINEN
                     IRGEDLYIIQSTSPPVNDNLMELLIAIDASKRASAKRITAVIPYFGYARQDRKDEGRT
                     SISAKLIANLITSAGANRVVTIDLHAGQIQGFFDIPLDNLFSVNALIEDMKKITDKRS
                     ELAIVSPDVGGVIRARAFAKRINASLVIIDKRREKGGESEVMNIIGDVKDKTCVILDD
                     IADSGGTLCNAAKALKNNGAQEIYSYIVHGVLSGDALERIKGSDIKELILTDTIQATE
                     SVKNIKNIRHISIAPLMGEAIKRIHSDSSVSALFD"
     gene            complement(2177..2956)
                     /gene="pgeF"
                     /locus_tag="EYO86_06160"
     CDS             complement(2177..2956)
                     /gene="pgeF"
                     /locus_tag="EYO86_06160"
                     /inference="COORDINATES: protein motif:HMM:PF02578.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIC42650.1"
                     /translation="MKTNYYMHKKLNISDIGYGFFTKSWCLSIDNNASLNCSHNTDDD
                     QNVVNKNINIAKKKLGLDKSKLKFISQTHSNQVAIIDKNNFSSTVEADGSITQDKDIS
                     LAVLTADCCPIFIYDSDINFICCLHAGWKGCLLNIVGSALEQIQTIQSNVNKIFAIIG
                     PCLNKANFEVKTDLKEQFVIKNSNYDKFFTNMKKTKKTLFDMRGLIQFQFNKNLVNNV
                     YHINFDTYENENLFFSHRRSTHDKTLPTGRMINIIGFKNNI"
     gene            complement(2958..>3449)
                     /locus_tag="EYO86_06165"
     CDS             complement(2958..>3449)
                     /locus_tag="EYO86_06165"
                     /inference="COORDINATES: protein motif:HMM:PF02636.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42651.1"
                     /translation="FTENKIIQDPATLSFLNKQDHNNILEISEKRDNYFHQICKYLRK
                     FGGVSLIIDYGYYDNPNNFTLQSISNHQRTNIFDNPGTQDITSLVNFKRLIEIAKWHN
                     LNIYTFCSQKEFLLSHGIEKRKNIILKKCNNKQKDIIEKGYERLIDEKNMGSLFKVLI
                     VTR"
BASE COUNT         1079 a          603 c          456 g         1311 t
ORIGIN      
        1 taaatcgttt gaaccaggat gacctatacc tattcttagt cgtttaaagt ttttgccaat
       61 cgcttcatca atgcttaata gaccgttatg tccaccatta cctcccccaa tttttatttt
      121 tatctttcct acagccaagt ctagatcatc gtgaatcaca ataattttag acttgggtat
      181 tttaaaaaaa tttactattt ctcttatggg ttgtcccgaa atgttcatat aggtcaaagg
      241 tttgcaaaat aaacactcgt tattatcaat agtaccttta tataattctt tttttctgtc
      301 tttttttatt aattcacaat tatttttatc aatgagagag tcaactacat caaaacctac
      361 attgtgacgg gtcaaaaaat atttttttcc gggatttcct aatccacaaa ttaaccacat
      421 aatgtttata taaatgtaga aattgaaatt gtctgcttat ttaggcttat cttcagcagg
      481 ctcagctttt ttatcttcgg aagaatcccc tgaatcctga tcttctttag aatcagtacc
      541 ttcctcagtt tcgccctcaa ttgcttctcc ttcaacttct tcttctggtg taggttcttc
      601 ttctacttca attgttggtg gaaccaaagt tgctaccaca aaatcacgat ctgtaatagt
      661 tggcttaact ccgtcaggca attcaatatt actaatttta atggcatcac caatttctgc
      721 tgaaactaaa tcatattgta atttttcagg tatattattg gcattgcaac taagttcaac
      781 cactcgacga accaagttta ataccccccc ttgttttaaa cctgggcaaa tatctctatt
      841 aagaaattct acttcgatct ctacagtcac ttttgtatca tcttggacac gtaaaaaatc
      901 aaaatgaatt aatttatctg taaccggatg atattgtaat tgtttgggta aaaccttttc
      961 ttttttccct tcaacatcaa gattgatgat tttagaatag aaaacacctg agtgcattaa
     1021 tttttgaaga gccttatctt caaaaacaat ttgcgaaggt tcagaatttt tgccatatat
     1081 gatgcccggc accattccct tttttaagag tgacttagtt gatccactat cctttgatct
     1141 agcaattact ttaatgttct ccatatttag tattcaaaag ttaatcaaac agagctgata
     1201 cagaagaatc actgtggatc cgcttaatcg cctcacccat taaaggagcg atactgatat
     1261 gtctaatatt ttttatattt tttactgact ctgtagcctg aatagtgtca gttaatatta
     1321 attctttaat atctgaacct ttaattcttt ctaaagcatc gccagataaa acaccgtgga
     1381 caatgtaaga atatatttct tgagccccat tgtttttcag tgcttttgca gcattacaga
     1441 gagtgccacc tgaatcggca atatcatcaa gaataacaca tgttttatct tttacatcgc
     1501 caataatatt catcacttct gattcccctc ctttttctct tcttttgtca ataataacta
     1561 atgatgcatt tattcttttt gcaaacgcac gagcccttat caccccccca acatcaggag
     1621 acactatagc tagttcactt cttttatctg ttattttttt catatcttca attaacgcat
     1681 tcacagaaaa tagattatca agaggaatat caaagaaacc ttgtatttga ccagcatgta
     1741 aatcaattgt tacaactcta ttagcacctg cagaagttat taagtttgca attaactttg
     1801 ctgaaattga agttctgcct tcatcttttc tatcctggcg agcatatcca aagtaaggaa
     1861 ttacggcggt aattcttttt gcagaagcac gttttgaagc atcaatagcg attagtaatt
     1921 ccattaaatt atcatttaca gggggtgaag ttgactgaat tatgtaaaga tcttctcctc
     1981 gaatattttc attaatcttt acaaaaactt caccatctgc aaattttttt attgtggcat
     2041 ctgataaatt aacgccaatg taggaagcta ttgcttctgc aagcatcgta ttactattgc
     2101 aggaaataat cttcatcttt ttaattatcc tttcattata gcagaatttt aatagatcaa
     2161 gattaaaaat tttacattaa atgttgtttt taaatcctat aatattgatc attcttcctg
     2221 tcggtaaggt tttatcgtgg gttgagcgac gatggctaaa aaatagattt tcattctcat
     2281 aagtatcaaa attaatatgg taaacattat taaccaaatt tttattgaat tgaaattgta
     2341 ttaatcctct catatcaaaa agagttttct tagttttttt catattagtg aaaaatttat
     2401 cataatttga attttttata acaaattgct cttttaaatc agttttgact tcaaagtttg
     2461 ctttatttag gcatgggcca ataatggcaa aaattttatt aacatttgat tgtattgttt
     2521 gaatttgttc tagtgcagat ccaacaatat ttaacagaca acctttccag ccagcatgca
     2581 ggcaacaaat aaaattaatg tcactatcat aaataaaaat aggacaacaa tctgctgtta
     2641 aaactgcaag actaatatct ttatcttgag taatacttcc atcagcctcc actgtagagg
     2701 aaaaattgtt cttatcaatt atagcaactt gatttgaatg agtttggcta atgaatttta
     2761 acttgctttt atccaaaccc aatttttttt tcgcaatatt aatattctta tttacgacat
     2821 tttgatcatc gtctgtattg tgactgcaat taagagatgc attattatca atggacaagc
     2881 accaactttt tgtaaaaaat ccataaccaa tatctgaaat atttaatttt ttatgcatgt
     2941 aatagttggt tttcatttta tctagtaaca attagaactt tgaatagtga gcccatattt
     3001 ttttcatcaa ttaatctttc gtagcctttt tcaattatat ctttttgttt attgttacat
     3061 ttttttaaaa ttatattttt tcttttttct ataccatgag acagtaaaaa ttctttttgc
     3121 gaacagaaag tatatatatt aagattatgc cactttgcaa tttcaattag ccttttaaaa
     3181 tttaccaacg atgtaatatc ctgagttcct ggattgtcaa aaatatttgt gcgttgatga
     3241 ttgcttattg attgtaaagt aaagttatta ggattatcat aatatccata atcaataatt
     3301 aaactaactc caccaaactt gcgtaaatat ttacagattt gatgaaaata attatccctt
     3361 ttttccgata tttctaaaat gttattgtga tcctgcttat ttaaaaatga taatgttgca
     3421 gggtcttgaa ttattttatt ttcagtaaa
//
LOCUS       DTVV01000516            2591 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_678496, whole genome shotgun sequence.
ACCESSION   DTVV01000516 DTVV01000000
VERSION     DTVV01000516.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_678496"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="EYO86_06170"
     CDS             complement(<1..293)
                     /locus_tag="EYO86_06170"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038878.1"
                     /note="catalyzes the formation of phenylpyruvate from
                     prephenate in phenylalanine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIC42652.1"
                     /translation="MKEKIKVAFQGEKGAYSHLACLDVFPNVDTVACSTFEEAFQLAK
                     DNSEYKIVIPIENSLAGRVADIHYLIPKYKLQIHAEHFQKESHNLLVIKGAKI"
     gene            complement(290..1027)
                     /gene="kdsB"
                     /locus_tag="EYO86_06175"
     CDS             complement(290..1027)
                     /gene="kdsB"
                     /locus_tag="EYO86_06175"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIC42653.1"
                     /translation="MKNTAIIIPTRLGAKRFPNKPLAKINNIPMIIHVLNRAKESKAG
                     EVFVATPDDAIFQVVEENGGKAILTKANHPSGSDRVYEAYIKELKNNVNLIINLQGDM
                     PNIKPNSISKLAKLMRGNNCDIGTLASYITDKNDIIDPNVVKVHIDQTLKGDNFLGAK
                     DFFRIKKNLNNEKIYHHIGIYAFTNIALTKYVKLARSKLEIKRNLEQLRALDNNMVIK
                     VGLSDSTPLGVDTEEDLIKVSKEMNKS"
     gene            1145..1675
                     /locus_tag="EYO86_06180"
     CDS             1145..1675
                     /locus_tag="EYO86_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HIC42654.1"
                     /translation="MNKIIVSIVLAVILILGINKITDAIFYVEKPEKSAYQITTATTA
                     ASKTVVDTSSESLDTGNIMALFASTSAADGAKVFKKCLTCHSIGKGDPNKIGPNIFGV
                     LNRKAGSISDYKYSKAMAAYGKVWSFEEMNGFLTKPKDWIKGTKMSFMGLKSAKDRAA
                     VILYMNENTDNPLPLQ"
     gene            complement(1667..2581)
                     /gene="argF"
                     /locus_tag="EYO86_06185"
     CDS             complement(1667..2581)
                     /gene="argF"
                     /locus_tag="EYO86_06185"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIC42655.1"
                     /translation="MRNFINLSDIDKRELRKIIDHAKSQKQKGSTIKTDVPLVGKTLI
                     MIFEKPSTRTRLSFELAMKKLGGDVLVLNPKESHYGSGDESIHDTAKILSQYGDVVMM
                     RTNKHEHFLEFSKHLEIPIINGLTNLSHPVQIMATVMTYEELRGSIANKKIAWLGDGN
                     NVVYSLIEAAAQFDFQLAIACPKKLEPNKKIVKWAKDKKANILITKDPKEAAEDASAV
                     ITDKFISMGDGVNKKKKKKLLKPYQVNKKIMKLAKPDSLFMHCLPASRGEEVTTEVMD
                     SKQSAVWLEALNRMHVQKSIIQFCLNLL"
BASE COUNT          819 a          449 c          363 g          960 t
ORIGIN      
        1 tcaatttttg caccctttat aactaacaaa ttgtgacttt ctttttgaaa atgctctgca
       61 tgaatttgaa gtttatactt tggaatcaaa taatgtatat ccgcaacccg tcctgctaaa
      121 gaattttcta ttggtataac aatcttatat tctgaattat cttttgctaa ttgaaaagcc
      181 tcttcaaatg tagagcaggc tactgtatca acattgggga aaacgtctaa gcatgccaaa
      241 tgagaatacg ctcctttttc tccttgaaac gccactttta ttttttcttt catgatttat
      301 tcatttcttt gcttactttt attaaatctt cttctgtatc tacgcctaac ggagtagaat
      361 cacttaagcc caccttaatt accatattat tatccaaagc tctcagttgt tccaaatttc
      421 tttttatttc taatttagat cttgcaagct ttacatactt tgttaaagca atatttgtaa
      481 aggcataaat tccaatgtga tgatagattt tttcattatt taaattcttt tttattcgaa
      541 aaaaatcttt tgcccccaaa aaattatcac cttttaacgt ttgatcgata tgaactttta
      601 caacatttgg gtcaataata tcgttcttat ctgttatata agaagccaga gtgccaatat
      661 cacaattatt acctctcata agcttagcta gttttgatat agaattaggt tttatattag
      721 gcatatcacc ttgtagatta attattaaat tcacattatt ttttagttcc tttatgtatg
      781 cttcataaac tcgatcgcta cccgaaggat ggtttgcttt ggtcaaaata gcttttccac
      841 cattttcttc aacaacctga aaaattgcat catctggagt tgcaacaaat acttcaccag
      901 cttttgactc ttttgctcta ttaagtacat gaataatcat tggaatatta ttaatttttg
      961 ccaaaggttt atttggaaat ctttttgcac ctaaacgagt gggtattatt attgctgtat
     1021 ttttcataaa taacaaatta tgcgtctaac cgtcgcaaaa caaacttatt taagttcatt
     1081 tttttcactt aagtcatgat atatcttaga attaagttgt actaaattta tatgcttttt
     1141 taaaatgaat aaaattattg tttcaattgt attagctgta atcctaattt taggaattaa
     1201 taaaattaca gacgcaatat tttatgtgga aaaaccagaa aaatctgcat atcaaatcac
     1261 caccgcaact actgctgcta gcaaaacagt agttgataca agctcggaaa gtttagacac
     1321 aggaaacatt atggcattat ttgcttcaac aagtgcagct gatggagcta aggtctttaa
     1381 aaaatgttta acgtgtcaca gtattggaaa aggagaccct aataagatag gaccaaatat
     1441 tttcggagtc ttaaatagaa aagcaggttc tatttctgat tataaatatt caaaagcaat
     1501 ggcggcctac ggaaaagttt ggtcatttga ggaaatgaat ggctttttaa caaaaccaaa
     1561 agactggata aagggcacta aaatgtcctt tatgggatta aagagtgcaa aagacagagc
     1621 tgcagtaatt ctctacatga atgaaaatac tgataaccca ttgcccctac aataaattga
     1681 ggcaaaattg aataatagat ttttggacgt gcattctatt taacgcttct aaccaaacag
     1741 ctgattgttt gctatccatt acttcagtag taacttcctc tcctctacta gctggtaaac
     1801 aatgcataaa caaagaatct ggtttagcaa gtttcataat ttttttattg acctgatatg
     1861 gttttaataa ttttttcttt tttttcttat ttacaccatc acccatggaa ataaatttat
     1921 cagttattac ggccgaagca tcttctgctg cttctttagg atctttggtt atcaaaatat
     1981 ttgctttctt atcttttgcc cacttaacaa tttttttgtt gggttctaac ttttttggac
     2041 aagctatagc taattgaaaa tcaaactgcg ccgcagcctc aattaaagaa taaacaacat
     2101 tattaccatc tcctaaccag gcaatttttt tattggctat ggatcctctt agttcttcat
     2161 aagtcattac tgttgccatg atttgaacag gatgacttaa gtttgttaaa ccattaataa
     2221 taggaatttc caaatgctta gaaaattcta aaaaatgttc atgtttattc gtcctcatca
     2281 ttacgacatc tccatattga gataaaattt ttgcagtgtc gtggatactt tcatctccgc
     2341 ttccataatg gctttctttc ggattcaaaa ctaaaacatc accacccaat tttttcatcg
     2401 caagctcaaa tgaaagtctg gttctagttg agggtttttc aaaaatcata attagtgttt
     2461 tgcccaccaa aggaacgtct gtttttatag ttgatccttt ttgtttttga gattttgcat
     2521 gatcaattat ttttcttaac tctcgtttat ctatatctga taaattaata aaatttctca
     2581 tatctaactc a
//
LOCUS       DTVV01000517            1176 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_684282, whole genome shotgun sequence.
ACCESSION   DTVV01000517 DTVV01000000
VERSION     DTVV01000517.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_684282"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1176
                     /locus_tag="EYO86_06190"
     CDS             <1..>1176
                     /locus_tag="EYO86_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIC42656.1"
                     /translation="KFSSQTKDKLVSSEVRQVVESVCLNKLEQWMEENPSEIKKVIDK
                     VLEASTAREAARKARELTRRKTGLESSSLPGKLADCQEKNPEESEIFIVEGDSAGGSA
                     KQGRDRKNQAILPLRGKILNVERANPKQILTSNEIGILIKAIGAGVGNPTGNEEEDKA
                     DGKFNLKKMRYHKIIIMTDADVDGSHIRTLLLTFFYRHMKPMIESGRLYIAQPPLYRA
                     KKGTSVQYLKDELEMDEYLINEGSKNLIFETPTSDNRVKKITGKDLFSIINLAKKSQK
                     LILPLTRRIDNKKVIEQASIIKALNQKNLNDPKIGIEIAKYLTLRLNTIESKIWNVKY
                     DDGKLIIIKEERGVKQTFEINKEFLITPEAKLLNDLRNELMDNFGILKNGCAGVIKNA
                     "
BASE COUNT          489 a          143 c          213 g          331 t
ORIGIN      
        1 aaaattttca agccaaacaa aagataagct tgtttcatca gaagttcgtc aagttgttga
       61 atcagtatgt ctaaataaac ttgaacaatg gatggaagaa aatccttctg aaatcaaaaa
      121 agtaattgat aaggtcctag aggcttcaac agcaagagaa gctgcaagaa aagctagaga
      181 gttaactaga cgtaaaacag gattggagag ttcttcgttg cctgggaaat tagctgattg
      241 ccaagaaaaa aatcctgagg aatcagaaat atttattgtt gaaggggatt ccgccggagg
      301 ttctgcaaag caaggaagag ataggaaaaa tcaagcaatt cttcctttaa gaggaaaaat
      361 tttaaatgta gaaagagcca atcccaaaca aattttaaca agcaatgaaa ttggaatatt
      421 aataaaggca attggtgccg gagttggaaa ccctactggc aatgaagaag aagataaagc
      481 tgatggtaaa tttaatctaa aaaaaatgag atatcacaag ataataatta tgacagatgc
      541 agatgtggat ggaagtcata ttaggacact acttttaact tttttttatc gtcatatgaa
      601 acctatgatt gagtctggga gattatatat tgctcaacca ccattatatc gtgcaaaaaa
      661 aggtacttca gttcaatatt taaaagatga attagaaatg gatgaatatt taattaatga
      721 aggttcaaaa aatttaatat ttgaaactcc cacatctgat aaccgggtaa aaaaaattac
      781 aggaaaagat ctctttagta ttattaattt ggcaaaaaaa tcccaaaaac ttattttacc
      841 tttaacaaga agaatagata ataagaaagt tatagaacag gcttcaataa ttaaagctct
      901 aaatcaaaaa aatcttaatg atccaaaaat tggaattgaa attgctaaat atttaactct
      961 tagactaaat acaattgaat caaaaatttg gaatgttaag tatgatgatg gaaaattgat
     1021 aataataaaa gaagaacgag gagtcaaaca aacttttgaa ataaataaag agtttcttat
     1081 aactcctgag gctaaattgc ttaatgattt gcgaaatgaa ttaatggata attttggaat
     1141 tttgaaaaat ggttgtgcag gcgttattaa aaatgc
//
LOCUS       DTVV01000518            1229 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_691410, whole genome shotgun sequence.
ACCESSION   DTVV01000518 DTVV01000000
VERSION     DTVV01000518.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_691410"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="EYO86_06195"
     CDS             complement(<1..924)
                     /locus_tag="EYO86_06195"
                     /inference="COORDINATES: protein motif:HMM:PF01960.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42657.1"
                     /translation="MIWGCYHMVKNRSTKDLSLSSNERLYHTKKTFSIFKFPIIKNLT
                     STHVKFYTFHTGFKKKREDLLIVVFDSSVPVAAAYSKTSTPSAPLIWDKKNNKGFCKI
                     LIVNSGNANAHTGMRGIKVIDNYVKRAAIKFNCSIEQILVSSTGVIGEQLDAIKIINK
                     FPLVKDSNNKDLLSAAKAIMTTDTFPKTIMKKIRIGSKNIRIFGFAKGSGMIAPNMGT
                     LLAYIFIEANFSKNVLKNLLHSHLETSFNSISVDGDTSTSDTIMLFSTVNPKDKKIVK
                     ETDIKKISKTLKDVMFQLALQVVSDGEGISKL"
     gene            929..>1229
                     /locus_tag="EYO86_06200"
     CDS             929..>1229
                     /locus_tag="EYO86_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIC42658.1"
                     /translation="MLNFVNKLFGSASKRQIKTYSKTIESINSFEEKLINLSNQELQD
                     KTEYFKNLIKGGSSLDDLLIEVFAVVREASKRTISLRHFDVQLIGGIILHQGMIAE"
BASE COUNT          420 a          183 c          184 g          442 t
ORIGIN      
        1 taattttgat atcccttcac catcagacac cacttggaga gccagctgaa acattacatc
       61 tttcaaagtt ttagagattt tttttatatc agtttccttg actattttct tatctttagg
      121 attaactgta gaaaataaca ttattgtatc acttgttgaa gtatctccat caacactaat
      181 cgaattaaaa ctagtctcaa gatgtgaatg taataaattt tttaaaacat tttttgaaaa
      241 atttgcttca ataaaaatgt acgctaacaa tgtacccata ttaggagcga tcattcctga
      301 ccccttggca aaaccaaaaa tacggatatt ttttgagcca attctaattt ttttcattat
      361 tgtttttgga aaggtatctg tagtcataat tgcctttgca gccgacagta agtctttgtt
      421 attggaatct tttacgaggg ggaatttatt gatgattttt attgcatcta attgctcacc
      481 aattacacca gttgaagaaa ctaatatttg ttcaatagaa caattaaatt ttattgctgc
      541 cctctttaca tagttatcaa taactttaat gcctctcatt cctgtatggg catttgcatt
      601 acctgaatta acaatcaaaa ttttacaaaa acctttgttg ttttttttat cccagataag
      661 aggtgctgaa ggagtagatg tctttgaata tgcagccgca actggaactg agctatcaaa
      721 aactacaatt aataaatcct ctcgtttttt tttaaaacct gtgtgaaatg tataaaattt
      781 aacatgagtc gaggttaaat ttttaattat tggaaatttg aaaatagaga aagttttttt
      841 ggtatgatat aatctttcat tacttgaaag gcttaaatcc ttggtagatc tatttttgac
      901 catgtgataa cagccccata tcataaaaat gttaaatttt gtaaacaaac tatttggatc
      961 tgctagcaaa agacagatta aaacttattc taaaacaata gagtccataa acagttttga
     1021 agaaaaatta attaatttat caaatcaaga gttacaggac aaaactgaat attttaaaaa
     1081 ccttattaaa ggaggctcat ctcttgatga tcttttaatt gaagtatttg cggtcgttag
     1141 agaggcttcc aaaagaacaa ttagcttaag acattttgat gtgcaattaa ttggtggaat
     1201 aattttgcat caaggaatga ttgctgaaa
//
LOCUS       DTVV01000519            1712 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_709550, whole genome shotgun sequence.
ACCESSION   DTVV01000519 DTVV01000000
VERSION     DTVV01000519.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_709550"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="EYO86_06205"
     CDS             complement(<1..1554)
                     /locus_tag="EYO86_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017971508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HIC42659.1"
                     /translation="MLNQEELIKKVKTYNRFFNPETLSKAYAFALNAHKNQKRDAGEP
                     FIIHPIAVADILTDLRLDSATITTGLLHDTIEDTKATYKTVEKEFGKEVADLVDGVTK
                     ISQLEGKVISSSKAENFRKLILATSKDIRVLLVKLADRLHNMRTLSSVPNLQKRIKKA
                     KETMEIYAPLADRMGMNSIRDELEDLSFQKLNPEARNLVVERLSTNKKNRENSFKEIS
                     KKFIKVLKNSGVNATIVGREKTPFSIWRKMQSKRVSLEQLTDIIGFRVIVDDVDACYK
                     ALGLFHTNWSTIPGRFKDYISTPKINSYKSIHTSVIGPNNERIEIQIRTQQMHDFAER
                     GIASHWVYKSSEKVSHLALKEYDWLRDLVEIMEKEKNTEHSLEYTKLQMFQDNVFCFT
                     PKGEVIKLPRGATPIDFAYAVHTKIGDRLTSCEINGRGSPLQSILKNGDLVHIIGSKN
                     ASPSPQWLSYAKTGKARASIRKYWQNSKNINQNSEKMYISSLCIKIPNVPGKLGEVSS
                     LIGFHQNNII"
BASE COUNT          502 a          288 c          239 g          683 t
ORIGIN      
        1 tattatattg ttttgatgaa aaccaattaa actacttact tcgcctagtt ttcctggaac
       61 attaggtatt ttaatacata aactgctaat atacattttt tcagaatttt gattaatatt
      121 tttactgttc tgccaatatt ttcttataga agctcgcgct ttgcctgttt tggcataaga
      181 caaccattga ggagacggtg aagcattctt tgatccaata atatgtacta aatctccgtt
      241 ttttaagatg ctttgcaatg gtgagcctct tccattaatt tcacatgaag ttaacctatc
      301 acctattttt gtatgcacgg cataagcaaa atctattggt gtcgcacctc ttggtaactt
      361 aataacctca cccttgggag tgaaacaaaa tacgttgtct tgaaacattt gaagttttgt
      421 gtactctaat gagtgttccg tatttttttc tttttccata atttccacaa gatctcttaa
      481 ccagtcatat tctttgagag ctaaatgact tactttttca gaagatttat atacccaatg
      541 agatgctata cccctttcag caaaatcatg catttgttgc gttctgattt gtatttctat
      601 tctttcatta ttaggcccta tcactgatgt atgtatagat ttataactat taatttttgg
      661 tgttgatatg taatccttaa atcttccagg tatcgtagac caattagtat gaaacaaacc
      721 aagtgcttta taacaagcgt caacatcatc aacaatgaca cggaaaccaa taatatctgt
      781 aagttgttca agagatactc tttttgactg catttttctc caaattgaaa aaggagtttt
      841 ttcacgtcct actattgtag catttacacc actgtttttt aaaactttta taaatttttt
      901 tgaaatttct ttaaaactat tttctctatt ttttttattt gtagaaagtc tttctaccac
      961 aaggtttctt gcttcaggat ttagtttttg aaaagataaa tcttcaagtt catctctaat
     1021 actattcatt cccatacgat cagccagagg tgcgtatatt tccattgtct cttttgcttt
     1081 ttttattctt ttttgcaaat ttggaacaga acttaaagtt ctcatgttgt gtaatctgtc
     1141 agctaattta acaagtaata ctcttatatc tttagatgtt gccagtataa gttttctaaa
     1201 attttcggct ttagagcttg agattacttt tccctctaat tgtgatattt ttgtaacacc
     1261 atctactaaa tcagctactt cttttccaaa ttctttttca acagttttgt aagtagcttt
     1321 agtatcttca atagtatcat gtaacaagcc ggtagttatt gttgcgctat caagccttaa
     1381 atcagttaaa atatctgcaa cagcaatagg atgtataata aatggttcgc cagcatctct
     1441 tttttgattc ttgtgagcgt ttagagcaaa agcataagcc tttgataatg tctcaggatt
     1501 aaaaaaacga ttataagttt tcactttttt aattaattct tcttggttta gcatataaat
     1561 attatatcaa gattttttaa tctttaatat agagtttttg tcttctttag gaagattctc
     1621 tatcaagaca cttataagtt ctttggtttt tggatgcttc caatccggaa gtatttcctg
     1681 cagtggatat aaaataaaat tcctataaat aa
//
LOCUS       DTVV01000520            1129 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_722774, whole genome shotgun sequence.
ACCESSION   DTVV01000520 DTVV01000000
VERSION     DTVV01000520.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_722774"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1129
                     /gene="topA"
                     /locus_tag="EYO86_06210"
     CDS             <1..>1129
                     /gene="topA"
                     /locus_tag="EYO86_06210"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIC42660.1"
                     /translation="PKLANLNIHRITFNEITKNAVLEALKEPRKVNENLVNAYLARRA
                     LDFLVGFNLSPVLWRKLPGSKSAGRVQSVALKIITEREFEIEKFNPEEYWSLKAIFNK
                     GQEKEFESRLIKFKDEKIEKLTIKTESEASNFLEKIQNSTYKVKEIQKKEIKRNPYAA
                     FTTSGMQIEASRKLGFSANQTMRTAQKLYEGTEINGEPTGLITYMRTDSVTMSKDAIR
                     EVRSFVLEKLGNEYIPAEPRIYKSKAKNAQEAHECIRPTNIKLSPEELKKYLNSDEYK
                     LYEIIWQRAVSSQMASAIIDQVLVDITNEQDIIVFRSNGSSIKFPGMLAVYQETKEED
                     KKEDEDDKSSLLPPLVQGDLLNLIKAEKNQHFTLPPPRYTEA"
BASE COUNT          459 a          151 c          193 g          326 t
ORIGIN      
        1 cctaaattag ctaatttaaa tattcataga ataacattta atgaaattac caaaaatgca
       61 gttctagaag ctttgaaaga acctagaaaa gtaaatgaaa accttgttaa tgcatattta
      121 gcaagaagag cgttggattt tctagttggt tttaatttat ctccagtttt atggagaaaa
      181 cttcctggaa gcaaatcagc aggtcgtgtc caatcagtag ctttaaaaat aattactgaa
      241 cgtgagtttg aaatcgaaaa atttaatcca gaagagtatt ggtcattaaa agctattttt
      301 aataaaggac aagaaaaaga gtttgagagt agattaataa aatttaaaga tgaaaaaata
      361 gaaaaattaa caattaaaac tgagtcagaa gcatcaaact ttttagaaaa aatacaaaat
      421 agtacataca aagttaaaga aatacaaaag aaggaaataa aaagaaatcc atatgcagca
      481 tttactactt caggtatgca aattgaggct agtagaaagt taggattttc agcaaatcaa
      541 acaatgagaa ctgctcaaaa attatacgaa ggtactgaga ttaacggtga acctacgggc
      601 ttaattacat atatgcgcac agacagtgtt actatgtcaa aggatgcgat aagagaagtt
      661 agaagttttg ttttagaaaa attaggcaat gaatatatac cggctgaacc tagaatttat
      721 aaatctaaag ctaaaaatgc tcaagaagcg catgaatgta ttaggcctac taatattaag
      781 ttaagtcctg aagaattaaa aaaatattta aattctgatg aatataaact ttatgaaatt
      841 atttggcaga gagcagtatc ttctcagatg gctagtgcaa ttattgatca agtattagta
      901 gatattacca atgaacagga tattatagtt tttagatcta atggatcttc aatcaagttt
      961 cctggaatgc ttgctgttta tcaagaaact aaagaagaag ataaaaaaga agatgaggat
     1021 gataaatcat ctttgcttcc tcctcttgtt caaggagacc tccttaatct tattaaagca
     1081 gaaaaaaacc agcattttac actccctcct cccagatata cggaggcta
//
LOCUS       DTVV01000521            3930 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_757227, whole genome shotgun sequence.
ACCESSION   DTVV01000521 DTVV01000000
VERSION     DTVV01000521.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_757227"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            254..805
                     /locus_tag="EYO86_06215"
     CDS             254..805
                     /locus_tag="EYO86_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42661.1"
                     /translation="MPSGSIYFSDRVGDTNPEVGIRFSAIISAEPQDDGTYTAVRRTF
                     DSSMPNGLLFSSDYKTLYVAQSDYRASEKRQLRAYPVNNDGSLGDYEVLHDFGPHRGI
                     DGMTLSSEGNIVACTGWEISGPGGMITVFDPKGRIIETHPTPAERPTNCTFVGEDLYV
                     SSIEGHLLVARNTGMTGYLLWPN"
     gene            858..2423
                     /locus_tag="EYO86_06220"
     CDS             858..2423
                     /locus_tag="EYO86_06220"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00571.26,HMM:PF01595.18,HMM:PF08450.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HIC42662.1"
                     /translation="MPEWNWTQIADHPTLTEGPVWDGSGLLYNECYANTTFRWDPKAN
                     ESAVWRENTGQANGMSFDRQGQLYVCEGDAHRVTRLDPENPGAEPMVISNPPAGGTLN
                     WPNDLAIDSQGRVYFTDPNYTGDPNNLPHESVYMTEHTFGGIWNAASKAKYVKMIIDN
                     KDSIISSLLLSNNLVNILASSLATAFFYDLFGLTGIFYATLLMTFLLVIFAEVLPKTY
                     SINKPTRTALIIAPTIYYLNKMLLPFVFLINKIVNLIIRNKDQKDLTDSDEQSEEELQ
                     GVIDLYQTSNPDSEHEKEMLQSILKLNDTIVEEVFTHRKNIYSIDINLGLDEIIKKIN
                     LSKFTRIPFWKNNPENIIGLLNIRSLDININNYQNDRKIILDKLSKPWFIPESTNLLD
                     QLVEFKKQREHLSFVVDEYGELLGVISLEDIIEEIVGEIVDEIDTPQQQFKLNSQNKI
                     IAEGNQNVKDLYKKFNLELPDIESSTIGGYIMDLAKKIPLYGEIINDKYFNYKVISHS
                     RKQILRVEISQIN"
     gene            complement(2416..2673)
                     /locus_tag="EYO86_06225"
     CDS             complement(2416..2673)
                     /locus_tag="EYO86_06225"
                     /inference="COORDINATES: protein motif:HMM:PF01722.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HIC42663.1"
                     /translation="MNRKKRIESLLLKNFINWKFTVIDNSAQHSGHNNFNGTQETHFC
                     IIIKKPFSSKDNRISIHRKIYKLLKDDFQNGLHALEIKIAN"
     gene            2697..3287
                     /locus_tag="EYO86_06230"
     CDS             2697..3287
                     /locus_tag="EYO86_06230"
                     /inference="COORDINATES: protein motif:HMM:PF00226.29"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIC42664.1"
                     /translation="MTRFKYDIAKNNLSWENHQIRLCDKEACEDKGDYRAPKSRSNLN
                     NYYFFCLKHVTKYNKSWDFYKGLSVDQIELSIRKDTVWDRPSWPLKGNPAKVMDQLKE
                     FLMYDYSLFEKEKEIHDFLRNKLVDENITNEEHKSLNILELKMPISVDEIKKRYKKLV
                     KIFHPDVNDDNKNAERKFKEITEAYKTLLKKFLRNE"
     gene            3280..>3930
                     /locus_tag="EYO86_06235"
     CDS             3280..>3930
                     /locus_tag="EYO86_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HIC42665.1"
                     /translation="MNDTKKNIDLSIVPDQEINTKEIFGIECPFKVCGFKSKNDHVPI
                     IDKDYKFDPETTRAILAGFADNRRVIIQGYHGTGKSTHIEQVAARLNWSCVRINLDSH
                     ISRIDLIGKDSIVIRDGKQITEFQEGMLPWAYQNPVALVFDEYDAGRPDVMFVIQRIL
                     ESDSKLTLLDQSRIINPNRFFRLFATANTVGLGDTSGLYPGTQQINQGQMDRWNIVT"
BASE COUNT         1315 a          758 c          771 g         1086 t
ORIGIN      
        1 gcaatggtat cgctaacaat aatagttcta gttacttcgg tagccgcatt aggtacagaa
       61 tcctgaacgt tgtacgttag tacataagtt gctggttcat agcggcatct ggaggtcaag
      121 agaatgccag aatccatgga ttctggcaac aaaaaaccct gcctgccggc agggttcggc
      181 ttgatcaggt ttgatcgccg tcctaccggc accacttgag atcgataatg atcagtaggt
      241 cgatctggcg atcatgccct cgggctcgat ctatttttcc gaccgcgttg gcgatacgaa
      301 tccggaagtg ggaatccgct tttccgcgat tatctccgcc gagccgcagg atgacgggac
      361 ctatactgcc gtgcgccgca cgttcgactc gtcgatgccc aacggcctgc tcttttcgag
      421 cgattacaaa acgctgtacg tggcccagag cgactaccgg gctagcgaaa agcgccagct
      481 acgggcctac ccggtgaaca acgacggctc acttggcgac tacgaggtgc tgcacgactt
      541 cggtccgcac cgtggaatcg atggcatgac gctgtcgagc gaaggcaaca tcgttgcatg
      601 cacagggtgg gagatatccg gccccggcgg catgattacc gtcttcgatc cgaagggcag
      661 gatcatcgaa acccacccga ccccagccga gcggcccacc aactgcactt ttgtcggcga
      721 agacctctac gtttcgtcta tcgaaggcca cctgctagtc gctcgcaaca ccggcatgac
      781 cgggtatttg ctctggccta attagaattc tgccatatcg acatatctac ccgcctgcga
      841 ccgaaagaaa acacaaaatg ccagagtgga actggactca aatagccgat caccccacgc
      901 tcaccgaggg gccggtctgg gacggctcgg ggttgctgta caacgagtgc tacgccaaca
      961 cgaccttcag gtgggacccc aaggcgaatg aaagcgcggt gtggcgagaa aacaccgggc
     1021 aggccaacgg catgtcgttc gaccggcagg gccagcttta cgtatgtgag ggcgacgccc
     1081 accgggtgac caggctcgac cccgaaaacc ccggcgccga gccgatggtc atcagcaatc
     1141 ccccggccgg cggaaccctc aactggccga acgacctcgc aatcgattcg cagggccggg
     1201 tctacttcac cgaccccaac tacaccggcg accccaacaa cctcccgcac gagtcggtct
     1261 acatgaccga gcacacattc ggcgggatct ggaacgccgc atccaaagcc aaatatgtca
     1321 aaatgattat cgataacaaa gatagcatta tctcatctct ccttctctca aataatcttg
     1381 ttaatatttt agcctcatca ttggcaactg ctttttttta cgacctattt gggctaactg
     1441 gaatatttta tgcaactctt ttgatgacat ttcttttagt tatttttgca gaagtattgc
     1501 ctaagacata ttccataaac aaacccacaa gaaccgcgct aataattgcc cctacaattt
     1561 attatctaaa caaaatgtta ctacctttcg tttttttaat taacaaaatt gtaaacttga
     1621 ttattagaaa taaagatcaa aaggatttaa cagactctga tgaacaatca gaggaggaat
     1681 tacaaggggt tattgatttg tatcaaacct ctaacccaga ttctgaacat gagaaagaga
     1741 tgcttcaaag tattttaaaa ttaaatgata caatagtaga agaggttttt acccatagaa
     1801 aaaatattta ctctattgat attaatttag ggttagatga aataataaaa aaaattaatc
     1861 tttcaaaatt tacgcgcata ccattttgga aaaataatcc tgaaaatatt ataggacttc
     1921 ttaatattag atctctagat attaatataa ataactatca aaatgacaga aaaattattc
     1981 tagataagct tagcaagcct tggtttattc ctgaatccac aaatttattg gatcaattag
     2041 ttgaattcaa aaaacaaaga gagcatttgt ctttcgttgt tgatgaatat ggggaattat
     2101 tgggcgtaat ttctctggaa gatatcatag aagaaattgt tggtgaaatt gttgacgaaa
     2161 tagacacgcc ccagcaacaa tttaaactaa atagtcagaa taaaataatt gctgaaggta
     2221 atcaaaatgt aaaggatcta tataagaaat ttaatcttga gttgccggac attgagtctt
     2281 caactatcgg gggatatatc atggatttgg ccaagaaaat acctttatat ggtgaaataa
     2341 taaatgataa atatttcaat tataaagtta tctcgcactc acgaaaacaa atattacgag
     2401 tagaaatttc tcaaattaat tagcaatttt tatttctaag gcatgcaacc cattctggaa
     2461 atcatctttt agtaacttat atatttttct atgaattgat attctattat ctttagaact
     2521 aaaaggcttt tttataatta tacaaaaatg ggtttcttgg gtaccattaa aattattgtg
     2581 cccactatgt tgagcagaat tatcaataac tgtaaatttc caattaataa aattttttaa
     2641 aagtaaagat tcaattcttt tttttcgatt catattttat ggtatataca aaataaatga
     2701 ctagatttaa atatgatata gcaaaaaata acctttcatg ggaaaaccac caaatacgtt
     2761 tatgtgataa agaagcttgt gaagacaaag gggattatag agcgccaaaa tcaaggtcta
     2821 atttgaacaa ttattatttt ttttgtttaa agcatgtcac taaatacaat aaatcatggg
     2881 atttttataa aggcctcagt gtggatcaaa tagaattatc tattcgcaaa gacacagtct
     2941 gggataggcc ttcatggcca ttaaagggga atccagccaa agtaatggat caattaaagg
     3001 aattcttaat gtatgattac agtctttttg aaaaagaaaa agaaatccat gatttcttaa
     3061 gaaataaatt agtagatgaa aatataacaa atgaggagca caaaagccta aatatattag
     3121 aactaaaaat gcctatttca gttgatgaaa ttaaaaaaag atataaaaaa ctagtgaaaa
     3181 tttttcaccc tgatgtaaat gatgataata aaaacgcaga aagaaaattc aaagaaatta
     3241 cagaagcata taaaacctta ttaaaaaaat ttttaagaaa tgaatgatac gaaaaaaaat
     3301 attgatttaa gtatagttcc tgatcaggaa ataaatacaa aagaaatatt tggaatagaa
     3361 tgtcctttta aggtgtgtgg attcaagtca aaaaatgatc atgttcctat aattgataaa
     3421 gattataaat ttgatccaga aacaacacga gctatattgg caggctttgc agataacaga
     3481 agagttatca tacaaggata tcatggtacc ggaaaatcaa ctcacataga acaagtcgca
     3541 gcaagattaa attggtcatg tgtacgtatt aatcttgata gtcatattag ccgaattgat
     3601 cttattggaa aagattcaat cgttattagg gatggaaaac aaataactga atttcaagaa
     3661 ggtatgctac cttgggctta ccaaaatccg gtggcattgg tttttgatga atatgatgca
     3721 ggacgtcctg atgtgatgtt tgttatccaa cgaattttag agtcagacag taaattaact
     3781 ttacttgatc aatccagaat aattaatcca aacagatttt ttcggttgtt tgctacagct
     3841 aatactgttg gactaggtga tacttcaggt ctttatcccg gcacgcaaca aataaatcaa
     3901 ggtcaaatgg atagatggaa tattgttaca
//
LOCUS       DTVV01000522            1298 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_792858, whole genome shotgun sequence.
ACCESSION   DTVV01000522 DTVV01000000
VERSION     DTVV01000522.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_792858"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            232..915
                     /gene="pyrH"
                     /locus_tag="EYO86_06240"
     CDS             232..915
                     /gene="pyrH"
                     /locus_tag="EYO86_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIC42666.1"
                     /translation="MKTIVIKLSGILFSLNSDISRIKKITKILEEINRKGFKIIAIAG
                     GGIIARNIQNTARTMNADEAFLDQIGIDVSRLNAKLMIASTTKACQHIPKNLDEVVNL
                     LVSSNMVFSGGFSPGQSTNATAALIAERTKAKLFINTTDVDGVFTSDPIKNKKATLIK
                     SITITDLFKKLNPEIMKAGTYDLMDMLSLKIIERSKIPTVIIKCDSTRIKSSLSGQFI
                     GTKITFSKL"
     gene            complement(910..1239)
                     /gene="hisI"
                     /locus_tag="EYO86_06245"
     CDS             complement(910..1239)
                     /gene="hisI"
                     /locus_tag="EYO86_06245"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIC42667.1"
                     /translation="MTNSLIDKINFDKNQGLVPVIVQESKSNEVLMLAYANRLAIEKT
                     IKTGFGYYWSRTRNKLWKKGEESGNVQKVFEIKIDCDQDTLIYTVKQKGNACHNNTKT
                     CFQKNLL"
BASE COUNT          456 a          200 c          160 g          482 t
ORIGIN      
        1 acatcatgaa gtgctttttt tgctggaata tacaaagaaa ttaactcagt acctctacct
       61 gtttttacag atagctcttt caatatcctt ctagttttgt ataacgctaa cgaatctata
      121 ttatttacca aaaattcacc tttattataa taatgaagaa attatatgat atattatatc
      181 atatttatta ataaattaat aatataatca aaacatatca tagatgatag aatgaaaaca
      241 atagtaataa aactgagcgg tatcttattt tccttaaatt cagatatttc tcgcataaaa
      301 aaaattacta aaattcttga agaaattaat cgtaagggct ttaaaattat tgccattgca
      361 ggtggaggta ttatagctag gaatattcaa aatacagctc gcacaatgaa tgccgacgaa
      421 gcatttttag atcaaatcgg tattgatgtt tcaagattaa atgcaaaatt gatgattgct
      481 agtactacta aagcctgtca acacatacca aaaaatctag atgaagttgt gaacttgctc
      541 gtttcttcta atatggtttt ttcaggtggt ttttcaccag gccaaagtac taatgctact
      601 gctgctttga ttgctgaaag aactaaagca aaattattta tcaacacaac agatgtcgac
      661 ggtgtgttta catctgatcc tattaaaaat aaaaaggcta ctctaattaa atcaattaca
      721 attacagact tatttaaaaa acttaatcct gaaattatga aagccggaac ttacgattta
      781 atggatatgc tttcactaaa aattattgaa agatcaaaga ttcctactgt aattattaaa
      841 tgtgatagta caagaatcaa atcttcatta tctggtcaat ttattggaac taaaattact
      901 ttttcaaaac tataatagat ttttttgaaa acaagttttt gtattattgt gacatgcatt
      961 ccctttttgc ttaaccgtat aaattaatgt atcttgatca caatctattt ttatttcaaa
     1021 aactttttgt acattacctg attcttcacc ttttttccat aacttatttc ttgttctgct
     1081 ccaataatat ccaaaacctg ttttgattgt tttttctatc gccagtctat ttgcataagc
     1141 gagcattaat acttcatttg atttactctc ttgtactatt acaggaacta atccttgatt
     1201 tttatcaaaa tttattttat cgattaatga gtttgtcatg ttcttacaag cacctgattt
     1261 tttaataaaa atttctttac taccgaaata gaaaattc
//
LOCUS       DTVV01000523            1193 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_795735, whole genome shotgun sequence.
ACCESSION   DTVV01000523 DTVV01000000
VERSION     DTVV01000523.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_795735"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="EYO86_06250"
     CDS             <1..505
                     /locus_tag="EYO86_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC42668.1"
                     /translation="LNPKKTILNTISLPLKIHNVVNKNQITERVKKLLSYVGLEPEFM
                     VRYPDTLSGGQKQRVAIARALAAEPRVLILDEPTSALDVIVQSKVLNLLLKLKKEFNL
                     TYIFITHDLSVVRNIANKVIVMNKGKIEESGNTKNVFLNPQSQYTKELIKAIPTVLDE
                     EDALKPK"
     gene            486..998
                     /locus_tag="EYO86_06255"
     CDS             486..998
                     /locus_tag="EYO86_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42669.1"
                     /translation="MRLSPNNKDIINLLSNTGKLSAKEFYKKLDKIIKKNIDHKLFTL
                     TVVDRSIKYVERVYSNNTKIYPLLGTKPIPKNSWTNKVINNKKYFFGESKNQIKKLFF
                     DYEKIFSLGCGSIINIPIINDKKILGTLNILHKEQFYTIKSVKMIQPYAQLLSPYFIL
                     HQLKMKKKKR"
     gene            complement(1017..>1193)
                     /locus_tag="EYO86_06260"
     CDS             complement(1017..>1193)
                     /locus_tag="EYO86_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIC42670.1"
                     /translation="DPYVMRNNQEVLEAGMVITIEPGLYKQGSLGVRIEDNILITDSG
                     CESLTSFSRDLTVI"
BASE COUNT          479 a          173 c          163 g          378 t
ORIGIN      
        1 attaaatcca aagaaaacaa ttctaaacac tatttcttta cctttaaaga ttcacaatgt
       61 tgttaataaa aatcaaatta ctgagagagt taaaaaactt ctttcgtatg tagggttaga
      121 gccagaattc atggtaagat atcctgatac tttgtcagga ggtcaaaaac aacgagtagc
      181 aattgcaagg gcattagctg cggaaccaag ggtgctgatt ttagatgaac ctacttctgc
      241 cctcgatgta atcgtgcaat caaaagttct taatttgctt ttgaaactaa aaaaagaatt
      301 taatttgaca tatatattta taactcatga tttaagtgta gtgcgcaata tagcaaataa
      361 agtaatagtt atgaataaag gtaaaattga agaatctggt aatacaaaaa atgttttctt
      421 gaatccacaa agtcaataca caaaagaact tatcaaagct ataccaactg ttttagatga
      481 agaagatgcg cttaagccca aataataaag atattatcaa tctactatct aatacgggaa
      541 aattaagcgc taaagaattt tacaaaaagc tggataagat aataaaaaaa aatattgatc
      601 acaaattgtt tactctaaca gttgttgacc gttcaataaa gtacgttgaa agagtatatt
      661 caaataatac taagatttac ccattactag gaacaaaacc cataccaaaa aattcgtgga
      721 ccaataaagt aattaataat aaaaaatact tttttggtga aagtaagaat caaattaaaa
      781 aattattttt tgattatgaa aaaatttttt ctcttgggtg tggttcaatt atcaatattc
      841 caataattaa tgataaaaaa atattgggta ctttaaatat tttgcataag gagcaatttt
      901 atacaataaa aagtgtaaaa atgattcaac cctatgctca actattaagt ccttatttta
      961 tccttcatca attaaaaatg aaaaaaaaga aaagataata acaataagta tttcttttat
     1021 attacagtta aatcccttga aaaacttgtg agactttcgc aaccactatc agtgattaaa
     1081 atattatcct ctattctaac gcctaagcta ccttgtttat acagtccggg ttcaattgta
     1141 ataaccatgc cagcttctaa aacttcctgg ttatttctca taacatatgg atc
//
LOCUS       DTVV01000524            1566 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_812717, whole genome shotgun sequence.
ACCESSION   DTVV01000524 DTVV01000000
VERSION     DTVV01000524.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_812717"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="EYO86_06265"
     CDS             <1..262
                     /locus_tag="EYO86_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIC42671.1"
                     /translation="MDELSEDDKLTVTRARKIEKFLSQPFFVAEVFTATPGKYVELED
                     TIKSFDGLVKGEYDHLPEAAFYMVGGIEEAIEKAKKLEAEAA"
     gene            263..661
                     /gene="atpC"
                     /locus_tag="EYO86_06270"
     CDS             263..661
                     /gene="atpC"
                     /locus_tag="EYO86_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIC42672.1"
                     /translation="MATISFDLVSPEQLLFNDEAGMIIVPGKDGDIGVLPRHSKVLSS
                     LRPGRVMVYGENKNLLKSFFVTRGFVEINPEKCIVLAEGVNEMSDLDKSSIQQQIQEL
                     ESGASDSAKEELAIAQSKIAALDSTYYEKL"
     gene            711..1343
                     /locus_tag="EYO86_06275"
     CDS             711..1343
                     /locus_tag="EYO86_06275"
                     /inference="COORDINATES: protein motif:HMM:PF01810.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42673.1"
                     /translation="MLDLNISIINFIPPLGFFILNVFVPGPNVLNTIATSMGSGRSSG
                     VACALACGLGLFISALVALFGAALLFYKIPIIYRGLTVFGGVMLLYFAQRYIRKSLSP
                     SEKLIVLQNIDKKTAFQQAFLVVMSNPKVMTTWLTVISLFPFIASNGYNVLIFSVMAG
                     TASFTGHFIFATVFSSSLASQIYMQFYRPINGIVGIGFIFYGMKLFLSLL"
BASE COUNT          488 a          233 c          303 g          542 t
ORIGIN      
        1 catggatgag ttgtccgaag atgataaact gacagtgact cgggcaagaa aaattgaaaa
       61 attcttaagc caaccatttt ttgtagctga agttttcact gctacaccag gtaaatacgt
      121 tgagcttgag gataccatta aaagttttga tggattggtt aaaggcgaat acgatcattt
      181 gccagaagca gccttctaca tggtaggtgg tattgaagaa gcaattgaga aagcaaaaaa
      241 attagaagcg gaagcagcat agatggcaac tatttctttt gatctagttt ctcccgaaca
      301 acttctcttt aatgatgaag ctggtatgat tattgttcca ggaaaggacg gtgatattgg
      361 cgtacttcct aggcatagca aagtattatc ttctcttcga ccaggacgtg tcatggtgta
      421 tggagaaaat aagaatttat taaaatcttt ttttgtcaca agaggttttg tagaaattaa
      481 ccctgaaaaa tgcatagttt tagcagaagg cgttaatgaa atgagtgatc ttgataaaag
      541 ttctatccaa caacaaattc aggaattaga aagtggtgcc agtgattcag caaaagaaga
      601 attagccatt gcacaatcaa aaatagcagc gttagattca acttattacg aaaaattata
      661 atcttattta tttttataat ctttaaggct tataaatacc ttaaataaca atgttagatt
      721 taaatatttc aataataaat tttattcctc cattgggttt ttttatactc aatgtttttg
      781 ttcccggacc aaatgtactt aatacaattg ccacctctat gggttctggt cgatcatcag
      841 gggttgcttg tgctttggcc tgtggattag gcttgttcat atcagcttta gtagctttat
      901 ttggtgccgc gttattgttt tataaaattc ctataattta tagaggccta actgtatttg
      961 gcggagtaat gttattatat tttgctcaac gttatattcg aaaatcatta tcaccttctg
     1021 aaaaacttat tgtacttcaa aatattgata aaaaaactgc ctttcaacaa gcgtttttag
     1081 ttgtgatgtc taatcctaaa gtgatgacaa catggcttac ggttatttct ctatttcctt
     1141 tcattgcaag caatgggtat aacgtactta tattttctgt aatggccgga acagcatctt
     1201 ttacaggtca ctttattttt gcgacagttt tttcttcaag cttagctagt caaatatata
     1261 tgcaatttta cagacctatt aatggtattg ttggtattgg ttttattttt tatggaatga
     1321 agttgttttt aagtctttta taaaccttag ttcatagttg gaagtgcaag ggggttggaa
     1381 aacgttgaaa gcattacaaa gatatcttca catacaagcc gagcacttgg ctaggaaact
     1441 taaggaaagg gggtaacatg atttgttggc tactcggaaa tattatttgg tgggtcggat
     1501 taatttacat aatcatttgg tatttcttct aaaatcttta tatgcaaata gagggcacga
     1561 gggacc
//
LOCUS       DTVV01000525            1764 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_827556, whole genome shotgun sequence.
ACCESSION   DTVV01000525 DTVV01000000
VERSION     DTVV01000525.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_827556"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            5..>1764
                     /gene="uvrA"
                     /locus_tag="EYO86_06280"
     CDS             5..>1764
                     /gene="uvrA"
                     /locus_tag="EYO86_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HIC42674.1"
                     /translation="MKIIGAREHNLNSVDLEIPKNKLVVITGVSGSGKSSLAFDTVYA
                     EGQRRYVESLSAYARQFLEIMQKPDVDSIEGLSPAISIEQKTTSKNPRSTVGTITEIH
                     DYMRLLFARIGIPYSPTTGKPIESQSVSQIVDNIMKKKIGEKYLLLAPLVRGRKGEYK
                     KEISEMIKKGFQRFKIDKKIYLYDELPELDKKFKHNIDVVVDRIKINNDIKNQLTDSV
                     EIALSISDGIIHFENYDTKKIEIYSTKFACPISGFAIEEIEPRLFSFNNPFGACSDCN
                     GLGYKSYFDPEMIVPNPKLSLAEGAIEPWNKTSNDFYRQTLQSLSDHFSFDMEIPFED
                     LDEKIKKILLYGYKDKKINFHYHSFRGTYPIEKTFEGVIPNLEKRWFKTENEYYRDEV
                     SKYLKYDTCESCNGYRLKSEALCVKIDKLHIGQISDYTIEQASTWFVKLEKKLNEKQS
                     EIARRILKEIKERFEFLNNVGLNYLTLSRNSRTLSGGEGQRIRLASQIGSGLTGVLYV
                     LDEPSIGLHQKDNIKLINALKKLRDLGNTVIVVEHDTETIESADHIIDLGPEAGINGG
                     YIVEQGELETILKNKNSITG"
BASE COUNT          730 a          216 c          295 g          523 t
ORIGIN      
        1 taaaattaaa attataggag caagagaaca caatttaaac agtgtagatt tagaaatacc
       61 aaaaaataaa ctagttgtaa taacaggtgt ttcagggtca ggcaaatcat ctcttgcttt
      121 tgacactgtt tatgcagaag gacagagaag gtatgttgaa agtttatctg cttatgcaag
      181 acaattttta gaaataatgc aaaaaccaga tgtggattca attgaaggat tgtcaccagc
      241 gatatctatt gaacaaaaaa caacttcaaa gaatcctcga tcaacagtcg gaacaattac
      301 tgaaattcat gattatatga gattgctttt tgctagaata ggaatcccat attccccaac
      361 cacaggaaaa ccaatagaat ctcaaagcgt ttctcaaatt gtagataata ttatgaaaaa
      421 aaaaattggg gaaaaatatc ttcttcttgc gcctttggtt agaggaagaa aaggagaata
      481 taaaaaagaa atatcggaaa tgataaaaaa aggatttcaa agatttaaaa tagataaaaa
      541 aatataccta tatgacgaat tgcctgaact tgacaaaaaa tttaaacaca acatagatgt
      601 tgttgttgat agaattaaaa taaataatga tattaaaaat caattaactg atagcgttga
      661 aattgctttg agtatatcag atggaataat tcattttgaa aattatgata caaaaaaaat
      721 agaaatttac tcaacgaaat ttgcgtgtcc tatatcaggt tttgcaatag aagaaattga
      781 acctagatta ttctctttta ataatccttt tggtgcatgc tctgattgta acggattagg
      841 ttataaatca tactttgatc cagaaatgat tgttcctaat ccaaaattaa gtctagcaga
      901 aggagcaatt gaaccatgga ataaaacatc aaatgatttt tacagacaaa cccttcagtc
      961 gctatctgac catttttctt ttgatatgga aattcctttt gaagatttgg atgaaaaaat
     1021 taaaaaaata ctactttatg gatataaaga caaaaaaatt aattttcatt atcattcatt
     1081 tagaggcacg tacccaatag aaaaaacttt tgaaggggtt attccaaatt tagaaaaaag
     1141 atggtttaaa actgaaaatg aatattatag agacgaagta tcaaaatatt taaaatatga
     1201 tacatgtgaa tcatgtaatg gttatagatt aaaatcagaa gctctgtgtg ttaaaattga
     1261 taaattgcat attggtcaaa taagtgatta cacaatagaa caagcttcaa catggtttgt
     1321 caaattagaa aaaaaattaa atgaaaaaca atctgaaatt gctagacgaa ttctaaaaga
     1381 aataaaagaa agatttgagt ttttaaataa tgttggatta aattatctga cattgtcaag
     1441 aaattcacga acattatcag gtggcgaggg tcaaagaata agactggctt cacaaattgg
     1501 atcgggatta acaggagtat tatatgtttt ggatgaacct tcaattggtt tacatcaaaa
     1561 agataatatt aaattaatta atgcactaaa aaaacttaga gatttaggta atacagtcat
     1621 agtagttgaa catgacacag aaacaataga gagcgctgat cacatcattg atcttggtcc
     1681 tgaagccgga attaatggtg gttacatagt agagcaaggt gaattagaaa caatattgaa
     1741 aaataaaaat agcattactg gaaa
//
LOCUS       DTVV01000526            6550 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_831768, whole genome shotgun sequence.
ACCESSION   DTVV01000526 DTVV01000000
VERSION     DTVV01000526.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_831768"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="EYO86_06285"
     CDS             complement(<1..1415)
                     /locus_tag="EYO86_06285"
                     /inference="COORDINATES: protein motif:HMM:PF01055.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42675.1"
                     /translation="MKIFSKILSKKGGKAGAVLLLTNGWQMRLRILEDWLLRITLEPS
                     DGLTINKTWMVCPKHPPGLSGRNRENLNCFKRPNNSFYCSKDKTSLIGGKFKVIIRNN
                     PLALEISILDNNNQWKTVLSDRETGSWYWDKKHKTTSHYQLRKKGDRLYGLGEKSGPL
                     ERSLRRFRMSQIDALGYNAEKSDPLYKSVPWLMVRSINNPECDVGLLYDTLSPAVFDC
                     GSEHSNYHSNYRYFKAETKGLDLYVLTRTQDQSLPAMLVKLTGMPPLPPRWSLGFQFS
                     SMELADHPKGERAFYDFLNHCKKFNIPISGLHFSSGYTLRQGKRYVFTWDHSRFPNPK
                     SLLNKIKSIGIHTVANIKPALLKDHPLYKKAAAQNIFLKKSSGTFHSVLFWGGKGSLL
                     DFTSKAAQKWWKKNIKEKLLNFGFDTVWNDNNEYDIEQDDIICDGDGNTINANEIIPA
                     QALLMVKASSEEISKQKPNKRV"
     gene            complement(1485..2501)
                     /gene="ugpC"
                     /locus_tag="EYO86_06290"
     CDS             complement(1485..2501)
                     /gene="ugpC"
                     /locus_tag="EYO86_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HIC42676.1"
                     /translation="MATLELKKLRKSYGKLEVIHGVNLEVEDGEFCVLVGPSGCGKST
                     LLRMVAGLESITDGDIVIDNKKVNDISAANRGLAMVFQSYALYPHMNVRQNLAFGLEN
                     LKMQKDEIETRIIEAARLLRMEEYLNRRPGQLSGGQRQRVAIGRAIVREPSIYLFDEP
                     LSNLDAELRVATRVELKALHQRLGNTMIYVTHDQVEAMTMADKIVVLNEGIIEQVGAP
                     LDLYNKPANKFVAGFIGSPRMNFFDINKLPESIKTIAPKNAITLGVRPEHFEVNGELT
                     LPLQVKNVEQLGADSFVYGYTIDGQELSIKFSDQTEIKVKQDISIGFSENRSHWFDNN
                     DFSI"
     gene            complement(2513..3901)
                     /locus_tag="EYO86_06295"
     CDS             complement(2513..3901)
                     /locus_tag="EYO86_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC42677.1"
                     /translation="MSFVDWISYGYLLFGFLIIFLPVLWLALNSIKSQFLLQKLDINL
                     LPLDYERVARAAVFGPEGKEIIIIKDLPNWVLYWNELTEDEKAQHEIQSIISEYSGNE
                     YYVLRSHFGLVAPSARQLIEKYNMPEWLIRYPSMFPNAKKEYKPKIYLNNLDEEEKRL
                     LSEFLGIDPYKPNTFTSQILVSAEDPETNSIKQYSVNRLKTTRDTVSGRLIDNPESGV
                     VRLSKDKIIAKKNLKPSWINYTDPLSNNVTGMNFDAVRCLNNSIFVTIVATIITVLIN
                     SMAAFALSKYRFNGQIIFYIIILATLMVPASVLIVGIFKMVAMTGLSGTLWGVIIPGA
                     ATPTGVFLLRQYMLTIPDELLEAARMDAASEWSIYWRIVFPLALPAIAVLTILSIVWR
                     WNDLILPMIAVSTTRAAYTIQLCLLDFQGEYVSQEHYRLAMTVVSLIPTTLVFVFLQR
                     YITTGIATTGIK"
     gene            complement(3949..5004)
                     /locus_tag="EYO86_06300"
     CDS             complement(3949..5004)
                     /locus_tag="EYO86_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC42678.1"
                     /translation="MINFFINNWNWPIVLFVYVLIGTLLSKGVVGLIPRTMSIVGWPI
                     ELTQRLTGTRSLPYLFLFPNILIFGLFTFLPLFMNFGFSVTDGESINFSSRDYSGSDN
                     LARLVLETQIDTGIENMEADKFKRSIADTLIFVIFQVPIMIVIALITAVVLNRKIVGR
                     GFWRAIYFYPVMLSPVVVAFLWTLILKRQGLLSQTLINWGWIEEPIQWLTDPSWTMFW
                     SVFVYTWAHLGFYMLILLAGLQSIPKDLYEAAEMDGTSNQRVFWRITLPLLMPILLVV
                     TVLSLIKAFQAFEELFAMQVSWISIVSYIFETAGLRGQKTTFGLGIAATASLLVALLL
                     ILLSLLQFYLTRKQVKL"
     gene            complement(5091..6365)
                     /locus_tag="EYO86_06305"
     CDS             complement(5091..6365)
                     /locus_tag="EYO86_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020024083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC42679.1"
                     /translation="MSDFASIFKPLGLIFFATLFILSFNVKAAELKFICYQDINECDT
                     IAEMAKSWESSTGNKVNIETVGYEVIREQLENQLESDAAPDLARVTNLGGLNKFYLDL
                     KPYVDSSYWETNYGATLPWYRKPSGDDGIYGWQTQLTVTGPYVNVTMFEDAGVDMPAE
                     GATWDDWADALRKVKSELGLTAGLALDRTAHRWAGPAFSYGAKFHKDGVPILVDEGFR
                     KFSEVFVGWHKEGLMPAEGWPAGSGTAYKNAAPLFLDGTVAMHMSGSWMLGNYAKNIT
                     DFEWKVVPIPCGPGGCGAMPGGSGVVAFKSTKHPEAAASFIDFLSQTENAETFAASTS
                     NITAHQGLQKSGIDYADVSPVVAEGLAIFAANAGKAADTTPQAYWFQGYNKNFAIYGI
                     VPDYITKAITGEMSLDDALKAIDADVAAKIAE"
BASE COUNT         2153 a         1233 c          978 g         2186 t
ORIGIN      
        1 actcttttat ttggtttttg ttttgaaatt tcttcacttg aagctttaac cattaataaa
       61 gcttgggctg ggataatttc attagcatta attgtattac catcaccatc acaaataata
      121 tcatcttgtt ctatgtcata ttcattatta tcattccaaa ctgtatcaaa tccaaaatta
      181 agcaattttt ctttaatatt ttttttccac cacttttgag cagctttaga agtaaaatct
      241 aaaagacttc ccttacctcc ccaaaacaaa actgaatgaa aagtaccgga tgattttttt
      301 aaaaaaatgt tttgagccgc agctttcttg tacaaaggat ggtcttttaa taaagctggt
      361 ttaatgttag caacagtatg tatgccgata cttttaattt tgttcaaaag agattttggg
      421 ttaggaaacc tactgtgatc ccatgtgaac acatatcttt ttccttgtct taaagtatat
      481 ccagatgaaa agtgtaatcc gctaataggt atgttaaatt ttttgcaatg atttagaaaa
      541 tcataaaaag ctctttctcc ttttggatga tctgccaatt ccattgaaga aaattgaaaa
      601 ccaagtgacc aacgtggtgg taatggaggc atcccagtta atttaacaag cattgccggc
      661 aagctttgat cttgcgttct agttaaaaca taaagatcaa gacctttggt ttcagcttta
      721 aaatatcggt agttagaatg atagtttgaa tgttcactac cacaatcaaa aacagccgga
      781 cttaatgtat catagagtaa gcctacatca cattctgggt tattaattga acgtaccatt
      841 aaccaaggaa cagatttata taatggatcg cttttttcag cattatatcc aagagcatct
      901 atttgggaca tacggaatct tctgagactc ctctctaaag ggcctgattt ttctccaaga
      961 ccgtagagtc tgtcaccttt ttttcttaat tgataatgac tggtagtttt gtgttttttg
     1021 tcccaatacc aagagccagt ttccctgtca gataatacag ttttccattg attgttatta
     1081 tccagtattg atatttctaa cgctagagga ttatttctta taatcacttt gaattttcca
     1141 ccgattaaag aggtcttatc tttactgcaa taaaaagaat tatttggacg tttaaaacaa
     1201 tttaaatttt cacgatttct tccacttaac ccaggaggat gttttggaca aaccatccaa
     1261 gttttattaa ttgtcaaacc atctgaaggc tctaaagtta ttcttagcaa ccaatcttca
     1321 agaatccgca atctcatttg ccaaccatta gttaataata aaacagcacc tgcctttcct
     1381 cccttttttg ataagatttt agaaaagatt ttcatttttt aaccatttaa caaatgtgtg
     1441 accaatagat gataattata tatgaaaaaa tttatttgtt attctcaaat agaaaaatca
     1501 ttattatcaa accaatgaga ccgattttca gagaagccta tagatatatc ttgtttgact
     1561 tttatttcag tttgatcact aaacttaatt gataattctt gaccatcaat tgtatatcca
     1621 tatacaaaac tatcagcacc taattgctca acgtttttca cttgaagtgg aagagtaagt
     1681 tctccattta cttcgaaatg ttctggtcta acaccaagag ttattgcatt ttttggagca
     1741 atagttttta ttgattcagg taatttgtta atatcaaaaa aattcattct tggcgaacct
     1801 ataaaccctg cgacgaattt gttggcgggt ttattgtaaa gatcgagagg tgctcctact
     1861 tgctcgataa taccctcatt taatacaact atcttatcag ccatggtcat agcctctact
     1921 tgatcgtgag taacatatat cattgtattg cctaatcttt gatgcagtgc tttcaattca
     1981 actcgagtag ccactctcaa ttcagcatct aagttggata gtggttcatc aaataagtaa
     2041 atagaaggtt cgcgaacgat agcacgtcca atagcaactc tttgcctttg tcctcctgaa
     2101 agttgaccag gtcgtctgtt caaatattct tccattctca gtaatcttgc tgcctctatt
     2161 attctggttt cgatttcatc tttttgcatt tttaaatttt caaggccaaa agcaagattt
     2221 tgacgaacgt tcatgtgtgg atataatgca taagattgaa agaccattgc caaacctcta
     2281 ttggctgcgg atatatcgtt tactttctta ttatcaatca ctatgtctcc atctgtaatt
     2341 gattcaagac ctgcgaccat tcttaataaa gttgattttc cacatccaga aggtccaact
     2401 aaaacacaaa attctccatc ctcaacttct aaatttacac cgtgaattac ttccaatttg
     2461 ccataagatt ttcttaattt ttttaattcg agtgtagcca tataaaacct tgttatttta
     2521 ttcctgttgt tgctattccg gttgttatat atctttgaag aaaaacaaaa accaaagttg
     2581 taggaattaa actaacaaca gtcatagcta atctgtaatg ttcttggctg acatactctc
     2641 cttgaaaatc taataagcat aattgaatgg tataggcagc tcttgttgta gaaaccgcaa
     2701 tcattggtag aattaaatca ttccatctcc atacaatgga taaaatagtt aatactgcaa
     2761 ttgctggaag agcaagtgga aaaacaattc tccaataaat actccattca cttgctgcat
     2821 ccatcctggc tgcttctaaa agttcatcag gtattgttag catatattgc cttaacaaaa
     2881 agacacctgt cggggttgca gctcctggta ttattacacc ccacaaagtt cctgataatc
     2941 cagtcatagc taccatttta aatataccaa caatcaacac tgaggctggc accattaatg
     3001 ttgctaaaat aataatataa aaaataattt gaccattaaa acggtatttt gaaagagcaa
     3061 atgcagccat agaattaatt aaaacggtta ttatggttgc tactattgtt acaaatattg
     3121 aattatttaa acatctaacc gcatcaaaat tcatgccagt tacattattt gaaagtggat
     3181 cggtataatt aatccatgaa ggtttaagat tttttttggc aataatttta tcttttgata
     3241 aacgcacaac tccactctca ggattatcaa tcaatcggcc actaaccgta tcacgggttg
     3301 tttttaatct gttaacggag tattgtttta tactatttgt ttccggatct tccgctgaaa
     3361 ctaatatttg agaagtaaaa gtattgggct tataaggatc gattcctaag aattcagaaa
     3421 gcaatctttt ttcctcttca tccaagttgt ttaaatagat ttttggtttg tattcttttt
     3481 ttgcatttgg gaacatactt ggatagcgga taagccattc tggcatatta tatttttcta
     3541 ttaattgacg agcagaagga gccaccaacc caaaatgact tcttaaaaca taatattcat
     3601 ttcctgaata ttctgaaatg attgattgaa tttcatgctg tgctttctca tcttccgtga
     3661 gttcgttcca gtacagaacc cagtttggaa gatctttaat aataataatt tcttttcctt
     3721 ctggaccaaa gaccgctgct cttgcaacac gttcatagtc taatggaagt aaattgatat
     3781 ctaatttttg aagtagaaat tgcgatttta ttgaattaag tgcaagccat aatactggta
     3841 aaaaaattat taaaaaacca aacaaaagat aaccatagga tatccaatcg acaaaactca
     3901 ttttattttt gtgaccttgt tttctggtta aaaagctaat tacctgactc acagtttaac
     3961 ctgttttctt gtaagataaa attgcaataa tgaaagtaag attaaaagca aagcaactaa
     4021 aagagatgct gtggcggcta tgcccaaacc aaatgttgtc ttttgtcccc ttaaaccagc
     4081 agtttcaaaa atgtatgaaa caatagatat ccaactaact tgcattgcaa ataactcttc
     4141 aaatgcctgg aaagccttaa tgagagataa cacagtaaca accaatagta ttggcatcaa
     4201 cagaggtaat gttattctcc aaaacactct ttgattagaa gttccgtcca tctctgctgc
     4261 ttcgtaaaga tcttttggta ttgactgcaa tccagcaagt aaaataagca tgtaaaatcc
     4321 tagatgggcc catgtataga caaaaacaga ccaaaacatt gtccacgaag gatctgtcag
     4381 ccattgaata ggttcttcaa tccaccccca atttattaat gtttgagaca aaagaccttg
     4441 tcgtttaagt atcagggtcc aaagaaaagc aactaccact ggacttaaca tcacagggta
     4501 aaaatatatt gcacgccaaa aacctcttcc cacaattttt ctattaagca ccactgctgt
     4561 gataagagct ataacaatca taattggtac ttgaaaaatt acaaatatta aggtgtctgc
     4621 tatagatctt ttgaacttat cagcttccat attttcaata cctgtatcaa tttgagtttc
     4681 caaaactaat ctcgctagat tgtcagatcc ggaataatct ctagaagaaa aattaatact
     4741 ctctccatct gttacagaaa aaccaaaatt cataaaaaga gggagaaagg taaacaaacc
     4801 aaagatcaat atatttggaa ataaaaataa ataaggtaag ctacgtgtac cagttaatcg
     4861 ctgtgttagt tcaatcggcc aaccaacaat tgacattgtt ctaggtatga gaccaacaac
     4921 accttttgat aataatgttc caattaaaac ataaacaaat aatacaatag gccaattcca
     4981 attatttata aaaaaattaa tcatattggc tgcattataa acttaagtaa cacaaggtcg
     5041 ccaattttgg cgaccttaca tgtaaaataa attaatatct aaaaaaaaac ttattcagca
     5101 atcttagctg ctacatcggc atctatagcc ttcaatgcat catctaagct catttcacca
     5161 gttatagctt ttgttatata atcaggaact ataccgtata tggcaaagtt cttattatat
     5221 ccctgaaacc aataagcttg aggtgttgta tcggcagctt taccagcatt ggcagcaaaa
     5281 attgcaagac cttctgcaac aacagggctt acatcagcat agtcaatacc agatttttgt
     5341 aaaccttgat gcgcagtaat gttgcttgtg ctagcagcaa acgtttcagc gttttctgtt
     5401 tgacttaaaa aatcaataaa tgatgccgct gcttctgggt gcttagtaga tttaaaagct
     5461 acaactccag aaccacctgg cattgcaccg catccgccag gaccacaagg aattggaaca
     5521 actttccatt caaagtcagt tatgttttta gcataatttc ctaacatcca cgaaccagac
     5581 atatgcatcg ctactgttcc atctaagaaa agaggtgctg catttttgta agcagtacct
     5641 gagcctgctg gccaaccttc cgcaggcatt aagccttctt tatgccatcc aacaaatact
     5701 tctgaaaatt ttctaaatcc ttcatcaact aaaatcggta caccatcttt atgaaatttt
     5761 gcaccatatg aaaaggctgg tcctgcccat cgatgtgcag ttctatctag tgctaggcca
     5821 gctgtaaggc caagttcaga tttaactttt cttagtgcat ctgcccaatc atcccatgtt
     5881 gctccttctg ccggcatatc aacaccagca tcttcaaaca ttgttacatt cacataaggt
     5941 cctgttacag taagttgtgt ttgccatcca taaattccat cgtctccaga tggtttacgg
     6001 taccaaggca aagtagcccc atagttagtt tcccagtagg atgaatctac ataaggttta
     6061 agatctaagt agaatttatt tagaccacca agattagtta ctcttgctaa atctggggct
     6121 gcatcagatt ctagttggtt ttctagttgt tctctaataa cctcgtatcc aactgtttca
     6181 atgttaactt tgttaccagt ggatgattcc caagattttg ccatttcagc aatcgtatcg
     6241 cattcattaa tgtcttgata gcatataaac ttaagttctg ctgctttaac attaaaagat
     6301 aaaataaata aagttgcaaa aaaaataaga cccaaaggtt taaatattga tgcaaaatca
     6361 ctcattaaat agataaattt tttcattttc cctccatttt ttctacttta aaacataaag
     6421 caatttgttt taattaatac aaaaaaattt tttaaaaaaa taattttcgt ttttttaatc
     6481 tataatattt catataaaaa aaatactaaa ttattacaaa actttagcaa tcatatttaa
     6541 ttttttgcat
//
LOCUS       DTVV01000527            1037 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_833984, whole genome shotgun sequence.
ACCESSION   DTVV01000527 DTVV01000000
VERSION     DTVV01000527.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_833984"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            165..956
                     /locus_tag="EYO86_06310"
     CDS             165..956
                     /locus_tag="EYO86_06310"
                     /inference="COORDINATES: protein motif:HMM:PF05711.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42680.1"
                     /translation="MLLTLFSIVRTIIPNSRFKEFLRKKFKKQIIKERQKSAWGTSTN
                     APQQTKESVAFTLKLLERCCRLKGSVIECGVYKGGNSFQIAKKLKELKSDKNLYSLDT
                     FEGFPYDDYENMPKELLKEVYVDKKPKKFKGMHTDVDLEQIKKWFAENGLDNTIFLKG
                     LFVDTFKQILDEKFCFAYVDSDVYLSVKQCIEFLKNRMVLGGIIMFDDYNYKETLGCN
                     RAVDELLEKDSIVILDDERITTVNKDGTPIRAYWVKTRLNDRKEV"
BASE COUNT          392 a          120 c          187 g          338 t
ORIGIN      
        1 gcccaatttg tacaaattgt ggttcaaaaa agtttgagcg tcttgattct gatggcgtga
       61 aagttagatg tgttaaatgt aatagagaat acaagatata gtctattatc aatttttgac
      121 tagcctatac tactaaatct atatttgtag gttaatttta attaatttta ttgacattat
      181 tctctatcgt aaggacaata attcctaatt ctagattcaa agaatttctc agaaaaaaat
      241 tcaaaaaaca gattataaaa gaaaggcaaa aaagtgcttg gggcacatct actaatgccc
      301 ctcaacaaac aaaagaatca gtggccttta ctctcaaatt acttgaaagg tgttgtaggt
      361 taaaaggtag tgttatagaa tgtggggttt acaaaggagg caacagtttt caaatagcaa
      421 aaaaactcaa agagctgaaa tcagataaaa atctatattc acttgataca tttgaaggat
      481 tcccttatga tgattatgaa aatatgccaa aggaattatt gaaagaagtt tatgtagata
      541 aaaaacctaa aaaatttaag ggtatgcata ccgacgttga tttggaacag ataaaaaaat
      601 ggtttgctga aaatggattg gataacacaa tttttcttaa aggacttttt gttgatacgt
      661 ttaaacaaat tttagacgaa aaattttgtt ttgcctacgt agattcagac gtttatctaa
      721 gtgtcaaaca atgtattgaa tttctaaaaa ataggatggt tttgggcgga ataatcatgt
      781 ttgatgacta taactacaag gaaacactag gttgtaatag agcggtagat gaactattag
      841 aaaaggattc aatagttatt ttagatgatg aacgaataac tactgtcaat aaagatggta
      901 cgccaattag agcttattgg gtaaaaacta ggttaaatga tagaaaagag gtttagataa
      961 cgactaatac tttttttttt gacttcattt ttaagatata gattatatgt gtagaatctt
     1021 gaattttttc aatggca
//
LOCUS       DTVV01000528            1048 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_834773, whole genome shotgun sequence.
ACCESSION   DTVV01000528 DTVV01000000
VERSION     DTVV01000528.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_834773"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="EYO86_06315"
     CDS             <1..324
                     /locus_tag="EYO86_06315"
                     /inference="COORDINATES: protein motif:HMM:PF03601.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HIC42681.1"
                     /translation="TLKLGRTLWIVPLVLFSAWYFREKRERLGFPLFILFFILAVALN
                     SLLTPSAEVNHILKTINKICLLTGLFCIGTQIDRSAIQQISLKPLLLAVLIWSVVIPA
                     SLMVV"
     gene            392..>1048
                     /locus_tag="EYO86_06320"
     CDS             392..>1048
                     /locus_tag="EYO86_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015828317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIC42682.1"
                     /translation="MKKLFTLFIFALLYLFSLSIASHGSAESISINEDKDRVIVFPDT
                     KNYKTIVADLHTHSVFSDGHVWPNIRVEEALRDGLDALAITEHLEYQPHYQDIPHKDR
                     NRSYEEAKAAASGKKIIIISGSEITRDMPPGHINAVFIKDANKLFNIDLPKNDKEKEI
                     LSEKIREMQWGADEETKKHYILANMWPAEKAVEAANQQGAFLFWNHPDWYRQAPDGIA
                     R"
BASE COUNT          338 a          166 c          200 g          344 t
ORIGIN      
        1 actttaaagt tgggaagaac tttatggatt gtgcctttag ttttattttc tgcttggtat
       61 tttagagaaa agagagagag gttaggtttt cctcttttca ttctgttctt tatattagca
      121 gttgcattga attctttgtt aacgccttca gcagaggtca atcatatttt aaaaactatt
      181 aataaaattt gcttactgac gggtcttttt tgcatcggta cacaaattga ccgatctgcc
      241 attcaacaaa tttctttaaa gcccttattg ttggcggttt taatttggtc agttgttatc
      301 ccagcttccc tgatggttgt ttaattccca caatattgct gaattgagaa tctttttaag
      361 ccataataaa ttagccgaat taagagattt tatgaaaaag ttatttacct tgtttatatt
      421 tgcattactt tatttattca gtctcagtat tgcttctcat ggatctgctg aatctatatc
      481 aataaatgaa gataaagata gagttattgt ctttccagat acaaaaaatt acaaaactat
      541 tgttgctgac ttacacactc attcagtttt ttctgacgga catgtgtggc cgaacataag
      601 agtagaggag gctcttagag atggcttaga tgctttggcc attactgagc atttggaata
      661 tcaacctcat tatcaagata ttccgcataa ggacagaaac agatcttatg aagaagccaa
      721 ggcagcagca tcaggtaaaa aaataattat cataagcgga tctgagataa cccgtgacat
      781 gcctccaggg catataaatg cagtatttat taaggatgct aataagcttt ttaatataga
      841 tttacctaaa aatgataagg aaaaagaaat cttgagtgaa aaaattagag aaatgcaatg
      901 gggagcagat gaagaaacaa aaaaacatta tatattggcc aatatgtggc cagctgaaaa
      961 ggctgtagaa gctgctaatc agcaaggagc tttcttgttt tggaaccacc ccgattggta
     1021 tcggcaagct ccagatggaa ttgccaga
//
LOCUS       DTVV01000529            3092 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_858109, whole genome shotgun sequence.
ACCESSION   DTVV01000529 DTVV01000000
VERSION     DTVV01000529.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_858109"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /gene="thrS"
                     /locus_tag="EYO86_06325"
     CDS             <1..1688
                     /gene="thrS"
                     /locus_tag="EYO86_06325"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIC42683.1"
                     /translation="MAEAVQALFPNTQVTIGPAIENGFYYDFAKEDPFTVNDFSKIEK
                     KMLEIINQGKSFTREVWTRDQAINFFSKKGENYKTELINDLNADETITIYKQGEWLDL
                     CKGPHMPSTKYIGKAFKLMKVAGAYWRGDSSNAMLTRIYGTAWRNEKELTEYLNQLEE
                     AEKRDHRKLGKEMDLFHFQEEAPGAVFWHPKGWGLFQSLINYMRKRQDQAGYVETNTP
                     DLLDRSLWETSGHWEKFRGNMFTTEEKEGRIFAIKPMNCPGAVEIYKQGLKSYRDLPL
                     RISEFGKVHRYEPSGALFGLMRVRAFTQDDAHIFCTENQITSESKIVCDLILSIYKDF
                     GFTDVIIKYSDRPEKRVGEDHVWDKAEKALKEAVKATGLAYTINPGEGAFYGPKLDFI
                     LRDAIGRDWQCGTLQVDLNLPSRLGATYIDENGQKKTPVMLHRALFGSLERFTGILIE
                     HYAGNLPFWLSPLQIVVATITSDSDNYADNIFNQLRDKGLRVKKDIRNEKIGYKIREH
                     SIAKIPVIMVIGKKEIENKTVSLRKLGSEKTETFKIDEITKILFKESLSPYNK"
     gene            1694..2173
                     /gene="hisI"
                     /locus_tag="EYO86_06330"
     CDS             1694..2173
                     /gene="hisI"
                     /locus_tag="EYO86_06330"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIC42684.1"
                     /translation="MVDNFFKKRKSIKQVEESTDLAPKFDENGLIPVITTDFRSGEVL
                     MQGYMNYDAFAKTIVLGEAIYYSRSRKSLWHKGKKSGFIQKIKEILIDDDQDCVWLKV
                     EVIGGASCHVGYRSCFYRNIPFGKNIYKSKIILKFTEKEKVFDPKKIYKNALNPTKL"
     gene            2201..2905
                     /locus_tag="EYO86_06335"
     CDS             2201..2905
                     /locus_tag="EYO86_06335"
                     /inference="COORDINATES: protein motif:HMM:PF00685.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42685.1"
                     /translation="MMQNIGVSKKIIFISSYPKSGNTWVRCIISSFLNNQNGKFNFND
                     LSRTGLFSRESHFTHLKDLEYQENGNLNFNFISNNWIKAQQKINKESKGIRFFKTHSI
                     REISNKYFTDQTVCLGFIYIVRDPRDIAISYTHHCGGDLNVAIETMLYNKKNYASHHK
                     TNELISTWKQHLFSWNKFNSVPRLFIKYEDMLNDTRKILLQIIDFINSLTNLSIKKND
                     NFLENILNTTSFNNLR"
BASE COUNT         1190 a          412 c          558 g          932 t
ORIGIN      
        1 ttatggcaga agccgtacaa gcattatttc caaacactca agttactata ggcccagcaa
       61 tagaaaatgg attctattat gattttgcaa aggaagatcc atttactgtt aatgatttca
      121 gtaaaataga aaaaaaaatg ttggaaatca tcaatcaagg aaaaagtttc acacgcgaag
      181 tttggacgcg ggatcaagct atcaattttt tttctaaaaa aggggagaat tacaaaactg
      241 aattaattaa tgatctcaat gccgatgaaa caattacaat atacaagcaa ggtgagtggt
      301 tagatctatg caaaggaccg cacatgcctt caacaaaata tattggaaaa gcctttaaac
      361 taatgaaggt ggctggcgct tattggcgag gagattcaag taacgctatg cttacaagaa
      421 tctatggaac agcttggcgt aatgaaaaag aattaacaga atacttaaat caattagaag
      481 aggcagaaaa acgtgatcat agaaaattgg gtaaagaaat ggatttattt cattttcaag
      541 aagaggcgcc tggtgcggtt ttttggcacc ccaaaggatg ggggcttttc caatcactta
      601 ttaattatat gcgtaagcgt caagaccaag caggttatgt agaaaccaat actccagact
      661 tattggatag atccttatgg gaaacttcag gtcactggga aaaatttagg ggaaacatgt
      721 ttacaacgga agaaaaagag gggcgtatct ttgctattaa accgatgaat tgccctggag
      781 cggtcgagat ctacaaacaa ggattaaaaa gttataggga tcttccatta agaatatctg
      841 aatttggaaa agtgcatcgt tacgaaccat caggtgcatt atttggttta atgcgagtac
      901 gtgcctttac acaagatgat gcccacattt tttgtacaga aaatcaaatt acaagtgaga
      961 gcaaaattgt ctgtgacctt attctaagta tatacaaaga ctttggcttc acggatgtga
     1021 taattaaata ttccgataga cccgaaaaac gtgtaggcga agatcatgta tgggacaagg
     1081 cagaaaaagc tctaaaagaa gcagtcaaag ctacgggatt ggcctacaca atcaatcctg
     1141 gtgagggcgc attttatggt ccaaaattgg attttattct tcgggatgct attggtaggg
     1201 attggcaatg cggaacatta caagttgatt taaatctgcc aagtcgatta ggggcgacct
     1261 atattgatga gaatggccag aaaaaaacac ctgtgatgtt acaccgcgcg ctttttggct
     1321 ctttagaaag atttacgggt attttaattg aacattatgc cggtaattta cctttttggt
     1381 tatcaccttt gcagatagtt gttgcaacaa taacttctga ctcagacaat tatgccgata
     1441 acatttttaa tcaattaaga gataaagggc ttcgtgttaa aaaagatatt cgcaatgaaa
     1501 aaattggtta taaaatacgc gagcacagta ttgcaaaaat acctgtaatt atggtgatag
     1561 gaaaaaagga aatagaaaat aaaactgttt cattaagaaa attaggaagt gaaaaaactg
     1621 aaacatttaa aattgatgaa ataacaaaaa ttttgtttaa agaatctctc tccccatata
     1681 acaaataaca atcatggttg ataatttttt taaaaaaaga aaaagcatta aacaagttga
     1741 ggaatcaacc gatctagctc ctaaatttga tgaaaacgga ttaattccag taattacaac
     1801 cgactttaga tctggggaag tattaatgca aggatacatg aattatgatg cttttgccaa
     1861 aactatagta ctaggagaag caatttatta ttcaaggagt agaaaatctt tatggcataa
     1921 aggtaaaaaa agtggcttta ttcaaaagat aaaagagata cttattgatg atgatcaaga
     1981 ttgtgtatgg ttaaaagttg aagtaattgg tggtgcaagc tgtcatgtag gttatcgatc
     2041 atgcttttat agaaatattc cttttggtaa aaatatttat aaatcaaaaa ttattctaaa
     2101 atttactgaa aaagaaaagg tttttgatcc aaaaaaaatt tataaaaatg cattaaatcc
     2161 cacgaaatta taattaatca tcttctcaat taataatgat atgatgcaaa atattggagt
     2221 aagtaaaaaa ataatattta tttcttctta tccaaaatca ggaaatacct gggtgaggtg
     2281 cataattagt tcatttttga ataatcaaaa tggaaaattt aactttaatg atttaagcag
     2341 aactggactc ttttcaaggg aatcacattt tacacatttg aaagatttag aatatcaaga
     2401 aaatgggaat ctaaatttta attttatatc taataattgg ataaaagcac aacaaaagat
     2461 aaataaagaa agtaagggta ttagattttt taaaactcat agtattcgag aaatatctaa
     2521 taaatatttt actgatcaaa ctgtttgttt gggatttatc tatattgtaa gagatccaag
     2581 agacatagca atttcatata cacatcattg tggaggggat ctgaatgttg ctattgaaac
     2641 aatgctttat aataaaaaaa actatgcatc tcatcataaa acaaatgaac taatttcaac
     2701 atggaagcaa catctgtttt cgtggaataa atttaatagt gttccaaggt tatttattaa
     2761 atatgaagat atgttgaatg atacgagaaa gatattgtta cagataatag attttattaa
     2821 cagcctaaca aatttatcaa taaaaaaaaa tgataatttt ctagaaaata ttttaaacac
     2881 tactagtttt aataatttac gatgacttga aaataatttc ggatttaatg aagcaactaa
     2941 taattctcct tttttcagaa taggtatatc aaatcaatgg gaaacaggtc tttctcagaa
     3001 tcaaaaagat ttaattcaaa atgaacttaa aatacctatg aaacaacttg ggtacttggt
     3061 gtaatagtta taataggcaa ttatattatg aa
//
LOCUS       DTVV01000530            1156 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_906955, whole genome shotgun sequence.
ACCESSION   DTVV01000530 DTVV01000000
VERSION     DTVV01000530.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_906955"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(126..692)
                     /locus_tag="EYO86_06340"
     CDS             complement(126..692)
                     /locus_tag="EYO86_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIC42686.1"
                     /translation="MFIQTEQTPNPQTLKFLPGRVVMTEGTAFFQNMDEAADSPLAKR
                     LFLLDGVKGVFFGSDFITITKPEDLDWQVIKPAILGAIMDHFNSEEATINKAVSNKGS
                     ESLKNDENDSDIIKQIKELLDTRVRPAVAMDGGDIIYQRFEDGIVYLHMQGACSGCPS
                     STATLKMGIENMLKHYVPEVVEVRPVDV"
BASE COUNT          338 a          227 c          187 g          404 t
ORIGIN      
        1 acaaatatgc ctgataatat ttgtagtgat ttttaaaaat tactgataga ttttttttaa
       61 acgattttgt tgtatattta attttatcat aagcacacaa atatgcttga taatatctat
      121 tacaactata catcaactgg tcttacctct acaacttcag ggacatagtg ctttaacata
      181 ttttcaatac ccatttttag tgttgccgtt gagctcgggc atcctgagca ggcaccttgc
      241 atgtgaagat agacaatacc atcttcaaaa cgctgataaa taatatcgcc gccatccata
      301 gccactgccg ggcgaacgcg cgtatccaat aattctttaa tctgttttat aatgtcagaa
      361 tcgttttcat cgttttttaa agattcagaa cctttatttg aaactgcttt gttgattgtt
      421 gcttcttcag aattgaaatg gtccataatt gcaccaagta tggctggttt tattacttgc
      481 caatccagat cttcaggttt tgtaatagta ataaagtcac ttccaaagaa aacgccctta
      541 acgccatcta agaggaagag tctttttgct aatggtgaat cagcagcctc atccatattc
      601 tgaaaaaaag cggtaccctc tgtcattact accctcccag ggagaaattt taaggtttgc
      661 ggattcggag tttgctctgt ctgtataaac atggctcctt tttatactta ttaatatatc
      721 ttgagtatga aaaaaaaaga aaattcttaa tttttgggga atacttacct agaaaaaccc
      781 actaaatcat gtacctgatc caaaacaggt tctgcaattc caatggcttt ttcttttccc
      841 attttcatta tcgaatccaa atatgtagca tcattcatta gtttttccat ctcgacagca
      901 attggggtga tttttgcaga agtgagatcg gctaaatctt ttttaaacat agcaaattcc
      961 tttcctgcgt attgatccac aactacatcc gttttagttt cacttaatgc cgcaaagatt
     1021 gttaagaggt taagtgcctc aggtcttttc tctgcctcgc ccatattcga aggaagtggg
     1081 caggggtctg tttttgcttt tctgattttt tgaacaatct catcctcatc atcacttaac
     1141 ataatacgtg aataat
//
LOCUS       DTVV01000531            1445 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_919646, whole genome shotgun sequence.
ACCESSION   DTVV01000531 DTVV01000000
VERSION     DTVV01000531.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_919646"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            109..1017
                     /locus_tag="EYO86_06345"
     CDS             109..1017
                     /locus_tag="EYO86_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIC42687.1"
                     /translation="MIKEDQVKSFKEKGYLVIENLLPENILKDLQKITEDFVEKSKFI
                     KENDSIFDLSDDHTPENPVVRRLKDPHLNHSVYSDIIKNNLITDVVSKLIGNNIRLEH
                     TKLNFKSAKGGEAIEWHQDWAFYPHTNDDIVEVGIFLDDCGNENGPLLCVPGSHKGPI
                     DDHHQNGYFIGAVDPSKSHYNLETAEPIVAKAGSISVHHVRCLHGSRKNNSEKSRRVL
                     FAGYCAADAWPLRGILDSGIKPNTSEGDFVGNVYSAFRERMIKGKSSLVARVEANPVR
                     MPYPPSPNIGSIYENQKEVLGRSFGE"
     gene            1021..>1445
                     /locus_tag="EYO86_06350"
     CDS             1021..>1445
                     /locus_tag="EYO86_06350"
                     /inference="COORDINATES: protein motif:HMM:PF00106.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC42688.1"
                     /translation="MTGRIQDRVAIITGAGSGIGEATAIRFAQEGAKVVIAEINSENG
                     KKVTDVINNKHGKAVFIETDVAKEESVKLMVDKTVKTFGAPDIMINNAGIAVFDDPLN
                     LSEENWRKCFSVDLDGVWYGCKRVLPHMLEKKKGSIVNIA"
BASE COUNT          529 a          194 c          299 g          423 t
ORIGIN      
        1 gatggggaat gagccaagaa caaattgata ataatccaag tgcaggcgga ttattttcag
       61 ttaaaccagg cgtcacaggt gtagctgata atcaatttgg tggatagaat gattaaagaa
      121 gatcaagtta agagtttcaa agaaaaagga tatttagtaa ttgaaaacct gcttcctgaa
      181 aatattttaa aagatttaca aaaaattact gaagattttg ttgaaaaatc aaaatttata
      241 aaagagaatg attcaatttt tgatctttct gatgatcaca cacctgaaaa tccagttgtg
      301 agacgattaa aagatcctca tcttaatcac agtgtctaca gcgatataat taaaaataat
      361 ttaataaccg atgttgtttc aaaacttatt ggaaataaca taagactaga acatacaaaa
      421 ttaaacttca aatctgctaa aggaggagag gctatagaat ggcatcaaga ctgggctttt
      481 tatcctcata ctaacgatga tattgtagaa gtgggcattt ttcttgatga ttgtggaaat
      541 gaaaatggac ctttattatg tgttcccggt tcccataagg gaccaataga tgatcatcat
      601 caaaatggtt attttatagg agctgtagac ccatccaaat ctcattataa tttagaaaca
      661 gcagagccta tagttgccaa agcgggatca attagtgtgc accatgttcg ctgtcttcat
      721 ggttcaagaa aaaataattc agaaaaatct cgaagagttt tatttgcagg ttactgtgca
      781 gctgatgcgt ggccacttag aggcatttta gacagtggta taaaacctaa tacatcagag
      841 ggagattttg ttggaaatgt ttatagtgct tttagagaac gcatgattaa aggaaaatcc
      901 tctcttgttg ctagagtaga agctaatcct gttaggatgc cgtacccacc ttctccaaat
      961 attggatcta tttatgagaa tcaaaaagaa gtacttggaa gatcatttgg agaataagta
     1021 atgacaggaa gaatacaaga tagagtagct ataataactg gagctggctc aggtattgga
     1081 gaagctacag ctataagatt tgcccaagaa ggagcaaaag tagttattgc tgaaattaat
     1141 tcagaaaacg gcaaaaaagt tacagatgtc ataaataaca aacatggaaa agcagtattt
     1201 attgaaacag atgttgcaaa agaggagtcg gtaaaattaa tggtagataa aacagtcaag
     1261 acttttggag cgccagatat aatgattaat aatgcaggta ttgctgtttt tgatgatcca
     1321 cttaatttaa gtgaagaaaa ttggagaaag tgtttttctg tagatttaga tggcgtttgg
     1381 tatggatgta agcgtgtttt accgcatatg cttgaaaaga aaaaaggaag tatagtcaat
     1441 attgc
//
LOCUS       DTVV01000532            1059 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_927805, whole genome shotgun sequence.
ACCESSION   DTVV01000532 DTVV01000000
VERSION     DTVV01000532.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_927805"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(77..385)
                     /locus_tag="EYO86_06355"
     CDS             complement(77..385)
                     /locus_tag="EYO86_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HIC42689.1"
                     /translation="MVKIIACKTTEIVSGQMKKITVGGKEIVVSNVDGNYFAIDDTCT
                     HSGASLSEGKLEGSIVTCDWHGAQFDCKNGKLIKFPGEIKDLKSYKIVIESENIFVEI
                     "
     gene            complement(389..>1059)
                     /locus_tag="EYO86_06360"
     CDS             complement(389..>1059)
                     /locus_tag="EYO86_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HIC42690.1"
                     /translation="KKFRLLSPIPNPSKIICLTFNYPNHAKEQNLESPKDPVIFFKPR
                     TSLCGTESEIKCPKFVKKLDYEIELAVIIGNTCKNIDESQSKEYTFGYMTFNDVSARD
                     IQMHDKQFTRGKSFDTFAPSGPWITSVDEVTDPQDLRLTTKVNGCVRQDSSTKNMFIK
                     IPSIISKLSKVMTLEKGDIIATGTPEGVALNNPDIPFLKDGDEIEMGIEKLGKIKNTV
                     RFTD"
BASE COUNT          300 a          196 c          152 g          411 t
ORIGIN      
        1 gcattatcaa gaaattttat attttcctct ttgcttacca tattacaatt tataccttat
       61 atctttaatt taaagcctat atctctacaa agatattttc agattcaatt actattttgt
      121 atgatttcaa atccttaatt tctcctggaa attttattaa ttttccattt ttgcagtcaa
      181 attgtgcacc atgccaatca caagtgacta ttgagccctc aagttttccc tctgaaagac
      241 tagctccaga atgagtgcaa gtatcatcga tcgcaaagta atttccatca acatttgaaa
      301 caacaatctc ttttccacca actgtaattt ttttcatttg acctgatacg atttcagttg
      361 ttttacatgc aatgattttt accacgtttt aatcagtaaa tcttacagta tttttaattt
      421 ttcccagttt ttctatcccc atctctatct cgtctccatc tttaagaaat gggatatctg
      481 gattattcaa ggctacacct tctggcgttc cagttgcaat tatgtcccct ttttctaaag
      541 tcattacctt acttagttta gatataatag atggtatctt tatgaacatg ttttttgtag
      601 atgaatcctg tctgacgcaa ccattcactt tggttgtaag tcgtaaatct tgtggatctg
      661 taacctcatc tacgcttgtt atccagggac cacttggtgc aaatgtatca aaagattttc
      721 cacgcgtaaa ttgtttgtca tgcatttgaa tgtctcttgc agaaacatcg ttgaaagtca
      781 tatagccaaa agtatactct tttgactggc tttcatctat gtttttacac gtattaccaa
      841 ttataacagc aagttcaatt tcataatcta acttcttaac aaattttggg cattttattt
      901 ccgattctgt tccacataga gatgttctag gcttaaaaaa tattactgga tcttttggag
      961 attcaaggtt ctgctctttt gcgtgatttg ggtagttaaa agtaagacag attattttag
     1021 acgggtttgg tataggagaa agtagcctaa attttttaa
//
LOCUS       DTVV01000533            1305 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_928237, whole genome shotgun sequence.
ACCESSION   DTVV01000533 DTVV01000000
VERSION     DTVV01000533.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_928237"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="EYO86_06365"
     CDS             <1..663
                     /locus_tag="EYO86_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HIC42691.1"
                     /translation="VISVFMPNKFYKDDDEYMEKLSLLMTNEYKAITHANIQLQLDCP
                     DLALARHMSYKSLSDEDFLKRAEKQIECLNNALVDIPADMIRMHICWGNYEGPHTHDI
                     SLEKILPIILKAKVKYLLIESSNPRHSHEWKIFGDIKLPHDKVLIPGLIDSTSNFVEH
                     PEVVADRLIQFSTVVPKDQLMAGTDCGFSTFAGFGKIDEEICYAKLNSLVEGAAIASK
                     KI"
     gene            complement(677..1303)
                     /locus_tag="EYO86_06370"
     CDS             complement(677..1303)
                     /locus_tag="EYO86_06370"
                     /inference="COORDINATES: protein motif:HMM:PF13407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42692.1"
                     /translation="MSATKPSDSFVTSCIENHIQVIAYNRNWNIPTTCSVACDHKGGG
                     EQVAQYFYDKGHHNIGIIKGPDDSFVSQQRCKGFINHLKTLGINKISTAKGSFTYEGG
                     REASKTLLRNKKLSALFCADDTMAFGCLDYIKNNTKLKIPGQLEIIGYDDKSVAEWIS
                     YNLTTIRQPIRQMAKLTTQLILDYIKDPEFEPVDHLVQGKFIKRKTTR"
BASE COUNT          425 a          214 c          227 g          439 t
ORIGIN      
        1 gtgatatcag tttttatgcc aaataaattt tataaagatg atgatgagta tatggaaaaa
       61 ttgtcattgc ttatgacaaa cgagtataaa gcaattaccc atgccaatat tcaattacaa
      121 ttagattgtc ccgacttggc tctagcgcga catatgagtt ataaatctct atctgatgaa
      181 gactttttaa aaagggcaga aaaacaaatt gaatgcctta ataatgcatt agttgatata
      241 ccagcagata tgattcgcat gcacatttgc tgggggaatt atgaaggccc ccatacacac
      301 gatatcagtc tagaaaaaat acttccaata attttaaaag ccaaagttaa atatctctta
      361 attgagtcat caaatccaag gcactctcat gagtggaaaa tttttggaga cattaaactt
      421 cctcacgata aagttcttat tcctggttta atagactcaa cttctaattt tgtagaacat
      481 cccgaggtag ttgctgatcg attgatacaa ttttcaactg tagttccaaa ggatcaatta
      541 atggcaggta ctgattgtgg ctttagtacc tttgctggat ttggaaaaat tgatgaagaa
      601 atttgctatg caaaattaaa ttctttagtt gaaggtgctg caatagcttc aaaaaaaatc
      661 taatattgca ttaaaattat cgagtagtct ttcgtttaat aaatttgccc tgtaccaaat
      721 gatcaactgg ttcaaattca gggtctttaa tgtaatcaag aattagttga gttgttaatt
      781 tagccatttg acgaatgggt tgtcgtatag tggtaagatt gtatgatatc cattcagcaa
      841 cggatttatc atcatagcct atgatttcta attgaccagg tattttaagt tttgtgttat
      901 tcttaatata atccaaacaa ccaaacgcca ttgtatcatc agcgcaaaaa agggcggata
      961 attttttatt tcttagtaat gttttagatg cttctcgacc accttcatac gtgaatgatc
     1021 ctttagcggt actgattttg ttaataccta aagtctttaa atgattgata aaacctttgc
     1081 atctttgctg gcttacaaaa gaatcgtccg gacctttaat gatgcctata ttatggtgtc
     1141 ctttatcgta aaaatattga gctacttgtt cacctcctcc cttatgatca caggcaactg
     1201 agcaagtagt cgggatattc caattgcgat tatatgcaat aacttgaata tggttttcaa
     1261 tacaacttgt aacaaaggag tcacttggtt ttgtagcaga cacga
//
LOCUS       DTVV01000534            3267 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_929085, whole genome shotgun sequence.
ACCESSION   DTVV01000534 DTVV01000000
VERSION     DTVV01000534.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_929085"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="EYO86_06375"
     CDS             <1..563
                     /locus_tag="EYO86_06375"
                     /inference="COORDINATES: protein motif:HMM:PF02321.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42693.1"
                     /translation="LLLAISDIENKKILLLKEKKSKKPSLDISGTAEYSDSGRIDSGT
                     KLTQGSVALTLTIPLYQKDQDNSNIRKYHSQILQSEIYLEDFREDIQIQIYNTYKDFK
                     ISESNMSTNQIVIQSIETSLNSLNEEYNIGTKTITDLVNEEEKLLNANVNYLNSKKNF
                     ITNYFKLKSLDGSLIKLFEHYIPATN"
     gene            609..890
                     /locus_tag="EYO86_06380"
     CDS             609..890
                     /locus_tag="EYO86_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42694.1"
                     /translation="MSEQQSIKDTLDVIRKALEDDESSLEEIKDNVLILNKLVKEDGT
                     IDVIEDNSLTKSEIKEILDNKLEELFDQHFEKWLNSKLPAHLEKYFNKK"
     gene            901..>3267
                     /locus_tag="EYO86_06385"
     CDS             901..>3267
                     /locus_tag="EYO86_06385"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIC42695.1"
                     /translation="MQLEKQFNYIKEEALIYKNWENSGSFKPKKNSEPYSIMMPPPNV
                     TGSLHMGHALNFTLQDILIRFHRMKGKEVLWQAGTDHAGIATQMVVERQLQEKNLTKN
                     DLGREKFINKVWEWKKESGGKISNQLRRLGASADWSRERFTMDESLSKAVKKVFVELY
                     KDKIIYKDKRLVNWDPKLLTAISDLEVEQKEHEGSLWHIKYPIDNDSHIVVATTRPET
                     MLGDTAVAVHPEDSRYKNYVGNTCILPLTNRKIKIIADEYADPEKGTGAVKITPAHDF
                     NDFEVGKRHDLEFINIFDEYAKCNEFTPKQFQGLDRFEARKKIIQELTNLNLLLKEEK
                     QLMVVPYGDRSGVIIEPWLTDQWFCDAKKLSVKPILAVKNGDTEIIPKQWEKTFYNWM
                     ENIQPWCISRQLWWGHQIPAWYGPDKKIFVAENVDEAKKEAFNYYKKDTVLFQDGDVL
                     DTWFSSALWTFSTLDWPEKSYELERFYPGNVLVTGFDIIFFWVARMMMMGSHFMKNTP
                     FKQVYIHPLIRDEKGQKMSKSKGNVIDPLDLLDKYGADTLRFTLAALMNPGRDVKLSE
                     SRVKGYKSFTNKIWNASIFLKINNCNLDLNFKYSEAQLPVNQWIINELASTVKKIDDF
                     MSKYLFHEVANELYHFIWHTYCDWYVEFAKSLFQDDSNKSKETKDVSIWVFLEILKIA
                     HPIMPFITEKIWNSHFNKDEYLMNQKYINIPIQKNYIQSQFNFKNLTQIISAIRNLRS
                     ELNIPYKDKIDLFINNSDNDFINFLNTFKNEIIHLLKLNNLSFNTIFSK"
BASE COUNT         1263 a          435 c          525 g         1044 t
ORIGIN      
        1 acttactttt agcaataagt gatattgaaa ataagaaaat tctactttta aaagaaaaaa
       61 aatctaaaaa gcctagtttg gatataagtg gaacggctga atattcggat agtggaagga
      121 ttgattcagg tacaaaactc acgcaaggtt ctgtagcttt gactctcaca atacccttgt
      181 atcaaaaaga tcaggataat tctaatataa gaaaatatca ttcacaaatt cttcagtcag
      241 aaatttattt ggaagatttc agagaggata tacagataca aatttataat acctataaag
      301 attttaaaat tagtgaatca aatatgtcaa ccaaccaaat tgtaattcaa tcaattgaaa
      361 cgtctttaaa tagcttaaat gaggaatata acattggaac taaaacaatc actgatttgg
      421 ttaatgagga agaaaaactt cttaatgcta atgttaatta tttaaattca aaaaaaaatt
      481 ttataacaaa ttactttaaa ttaaaatctc tggatggcag ccttataaag ttgtttgaac
      541 attatatacc tgcaactaat taatcattta aataccactt tgcataagat ataagtttct
      601 ttgaatagat gtctgaacaa caatcaataa aagacaccct tgatgttatt agaaaagctt
      661 tagaagatga tgaatcttct ttagaggaaa ttaaagacaa tgttttaata ttgaataaac
      721 ttgtaaaaga agatggaact attgacgtta ttgaagacaa ttcattaact aaatctgaaa
      781 ttaaggaaat tttagataat aaacttgaag aattatttga tcagcatttt gaaaaatggc
      841 ttaatagtaa attgccagct catttagaaa aatattttaa taaaaaataa tcaaggtaac
      901 atgcaattag aaaaacaatt taattatata aaggaggaag ccttaattta caaaaattgg
      961 gaaaactcag gatcattcaa gcctaaaaaa aattctgaac cctatagcat aatgatgcca
     1021 cctcccaatg tgacaggtag tttgcatatg ggccatgctt taaattttac tttacaagat
     1081 attctcattc gctttcatcg aatgaagggc aaggaagttt tatggcaagc aggaactgat
     1141 catgctggga tagcaacaca gatggttgtt gaaaggcaat tacaagaaaa aaatcttaca
     1201 aaaaatgatc ttggaagaga aaaatttatt aataaagttt gggaatggaa aaaagaatcc
     1261 ggtggtaaaa ttagtaacca attaagacgt ttaggtgctt cagcagattg gtctagagaa
     1321 cgtttcacca tggatgaaag tttgtcaaaa gctgttaaaa aagtttttgt tgaattatac
     1381 aaggataaaa ttatctataa ggataagaga cttgttaatt gggaccctaa attattaacg
     1441 gctatatctg atttagaagt tgaacaaaaa gaacatgagg gatctctttg gcatataaag
     1501 taccctattg ataatgatag ccatatagtt gttgcaacta ctcgacctga aacaatgctc
     1561 ggtgacacag ctgtcgcagt tcatcctgaa gactcaaggt ataaaaatta tgtaggaaat
     1621 acatgcattc ttcctcttac aaaccgaaaa attaaaatca ttgctgatga atacgctgat
     1681 ccagaaaagg gtacgggtgc tgtaaaaatt actcctgcgc atgattttaa tgattttgaa
     1741 gttggaaaaa gacatgattt agagtttatt aatatttttg atgagtacgc aaaatgtaat
     1801 gaatttacac ctaagcaatt ccagggatta gatagatttg aagcaagaaa aaaaatcatt
     1861 caagaactca caaatttaaa tctacttctt aaagaggaaa aacaattaat ggtagtaccc
     1921 tatggtgaca gatctggtgt tataatagaa ccttggttaa ctgatcaatg gttctgtgat
     1981 gcaaaaaaac tatctgttaa accaattctg gcagtcaaaa atggggatac tgaaattatt
     2041 ccaaaacaat gggagaagac tttctataat tggatggaaa atatacaacc ctggtgcatt
     2101 tcacgacagt tatggtgggg acatcaaata cctgcttggt atggacctga taaaaagatt
     2161 tttgtggctg agaatgttga tgaagcaaaa aaagaagcct tcaattatta taaaaaagat
     2221 actgttttgt tccaagatgg tgacgttcta gatacgtggt tttcatctgc tttatggaca
     2281 ttttcaacat tagattggcc agaaaaaagc tacgaattag aaagatttta tcctgggaat
     2341 gttcttgtaa caggttttga tattatattt ttttgggtag caagaatgat gatgatgggc
     2401 tcccatttta tgaaaaacac tccctttaag caagtttata tacatcctct tattcgtgat
     2461 gagaaaggtc aaaaaatgtc taaatccaag ggtaatgtta ttgatccctt agatttgcta
     2521 gataaatatg gagctgacac acttcgcttc actcttgcag ctttaatgaa tcctggaagg
     2581 gacgtaaagt tatctgagtc aagagttaaa ggttacaaat cttttactaa taaaatttgg
     2641 aatgcttcaa tattccttaa gattaataat tgcaatctag atttaaattt taaatattct
     2701 gaagctcaac taccagttaa tcaatggata attaacgaac tagcctctac agtaaaaaaa
     2761 atagatgatt tcatgtcaaa gtacttattt catgaagttg ccaatgaatt atatcatttt
     2821 atctggcata cttattgtga ttggtatgta gaatttgcca aatctttatt tcaggacgat
     2881 tcaaataaat caaaagaaac taaagatgtc tctatatggg tttttttaga aatattaaaa
     2941 atagctcacc caattatgcc ttttattact gaaaaaattt ggaactcaca ttttaacaaa
     3001 gatgaatatt taatgaacca gaagtatatt aatattccaa ttcaaaaaaa ttatattcaa
     3061 tcacaattta attttaaaaa cttaactcaa ataatatctg caataagaaa tttgcgttct
     3121 gaattaaaca ttccatataa ggataaaatt gacttattta taaataattc tgataatgat
     3181 ttcataaatt ttttaaatac ttttaaaaat gaaattattc atttattaaa attaaataat
     3241 ttgtctttta atactatttt ttcaaaa
//
LOCUS       DTVV01000535            1891 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_929352, whole genome shotgun sequence.
ACCESSION   DTVV01000535 DTVV01000000
VERSION     DTVV01000535.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_929352"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /gene="rlmJ"
                     /locus_tag="EYO86_06390"
     CDS             <1..452
                     /gene="rlmJ"
                     /locus_tag="EYO86_06390"
                     /inference="COORDINATES: protein motif:HMM:PF04378.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HIC42696.1"
                     /translation="FLKNSNIKIINDDGFRFPSKIKIKKQKKGIILIDPSYEEKDDHE
                     KVINLITKNYEQLENKIIIIWYPMINRNDTNNFIDGFKKTGIQNILRIEMPIQNDNEE
                     INMTGSGLLVFNAHNKTKQSLRGTIIELQKCLQLKGNKKKVIVNYLR"
     gene            471..1868
                     /locus_tag="EYO86_06395"
     CDS             471..1868
                     /locus_tag="EYO86_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIC42697.1"
                     /translation="MKLSSNSLKNAHFKFNMFSKSTSKKKPNLIEDPIMQLFIRIAVP
                     SSIGTVFMTLYNVVDTYFAGKISAEALAALAQTFPLYFIIIALGVGLSIGTTSLIANA
                     LGEKKTKKASYYLAQSIILAIIASVIVSLIGINLGPSIILIMNDSFVTMRLSMDYLNI
                     IFLGSIFVFIQMTVNSSLSAIGDTKSNRNVLIVSFFLNIVLNPLFIFGYGFIPAMGIK
                     GIAFSTIVSQALGTVYIIYKATKIELGKYLYLNCFKPKIKILIDLLKQGVPASIGMMM
                     ISVGIFIILFFISQYGDLALAGYGTAIRYEQLYLLPVLGLNIAVLSMVSQNFGAKQLN
                     RVKEIYNKAILFGCTFMFFSGFIIYFSAETAVSFFTNNKEVIKYGTTYLQITALMEPI
                     YPIFFISNALIQGLKKANVVMYLTLGRMVVLPVIVLWYLIFYLESSFEFVFWGQVMKL
                     IEEYSSRKHLSTITK"
BASE COUNT          675 a          256 c          283 g          677 t
ORIGIN      
        1 aatttttaaa aaacagtaat atcaaaatta ttaatgatga cggctttaga tttcctagca
       61 aaataaaaat taaaaaacaa aaaaagggta ttattttaat tgatccttct tatgaagaaa
      121 aagatgatca tgaaaaagtt ataaatctaa ttaccaaaaa ctatgaacag cttgaaaata
      181 aaattataat tatttggtat cctatgatta atagaaatga tacgaataat tttattgatg
      241 gttttaaaaa aacaggtatt caaaatattc ttcgtataga aatgccaata caaaatgata
      301 atgaagagat aaacatgact ggaagtggat tactagtttt taatgcacat aacaaaacta
      361 aacagagtct aagaggtaca attatagaac ttcaaaaatg tttacagtta aaaggaaata
      421 aaaaaaaagt aatagttaac tatttaagat aaaataactg tagatatttg atgaaacttt
      481 cttcaaatag tttaaaaaac gcccatttta aatttaatat gttttcaaaa tctacatcca
      541 aaaaaaaacc taatttaatt gaagatccaa taatgcaatt atttattaga atagcagttc
      601 catcaagtat tggtacagta tttatgactc tttacaatgt tgttgatact tattttgcgg
      661 gtaaaatttc ggctgaagct ttggctgcat tagcacaaac atttccttta tactttataa
      721 ttattgcttt aggagtcggc ctcagcatag ggacaacttc attaattgca aatgccttag
      781 gtgaaaagaa aactaaaaaa gcttcttatt atttagcgca atctataatt cttgccatta
      841 tcgcttctgt catagttagt ttaattggga ttaatttagg tccatctata atactaatta
      901 tgaatgatag ctttgtaact atgcgcttat caatggatta tctcaatatt atttttttag
      961 gctctatatt tgtttttatt caaatgacag ttaattcttc tctctcggca attggtgaca
     1021 ctaaaagtaa tagaaacgtg ttaattgtaa gttttttttt aaatattgtc ttaaatcctc
     1081 tttttatctt tggttacggt tttattcccg ccatgggaat taaaggcata gccttttcta
     1141 caattgtttc gcaagcatta ggaacagttt atataatata caaagctaca aaaatagaac
     1201 taggcaaata tctatattta aattgcttta aaccaaaaat taagatttta attgatcttt
     1261 taaaacaagg tgttccagca tcaataggaa tgatgatgat cagtgtgggt atcttcatta
     1321 ttttattttt tattagtcaa tatggagatc ttgcattagc tggttatgga acggcaatac
     1381 gttacgaaca attatatctt ttgcctgttc ttggattaaa tatagcagtt ttatccatgg
     1441 taagtcaaaa ttttggagcg aaacaactta acagagtaaa agaaatatat aacaaggcca
     1501 tattatttgg ttgtaccttt atgtttttta gtggctttat tatttatttt tctgcggaaa
     1561 ctgctgttag cttctttacc aacaataaag aagttataaa atacggcacg acatatctac
     1621 aaattacagc tttaatggaa ccgatctacc caatattttt catcagtaat gctttgatac
     1681 aaggtcttaa aaaagctaat gttgttatgt atttaacatt aggacggatg gttgttctcc
     1741 ctgtaattgt actttggtat ttgatatttt atttagaatc ctcttttgaa tttgtctttt
     1801 ggggtcaggt tatgaaactc atcgaggaat attcctcgag gaaacacctt tccaccatca
     1861 ccaagtagag acactgttcg aataagtggt c
//
LOCUS       DTVV01000536            1331 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_942608, whole genome shotgun sequence.
ACCESSION   DTVV01000536 DTVV01000000
VERSION     DTVV01000536.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_942608"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="EYO86_06400"
     CDS             complement(<1..1292)
                     /locus_tag="EYO86_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIC42698.1"
                     /translation="MDSITLEIIKGKLLSTADEMAVVLARTSMSPVIYEVLDFACGIC
                     DNKGNLVSQTNGVTLFTGTFSTQVKFIINKFSKNTVDGDIFLTNDPFKGGTHPCDFAI
                     IKPIFFKKKNIAFSISVAHWLDVGGSIAGSLPPDSDSMFKEGIRFSGVRLCRDNKLLP
                     DIASIILENVRFPKLAMGDLNAQIAAVKIADKRIKETYRKYSETTLNKAFNQILLDSE
                     KQSRRIISKLPDGIYKAKDYIDGDGNVKNQISVQIKIQIKGSNILFDYTGCPQARKAP
                     INCSLGALTSAVKTVFKALVEPFAPSNEGYFKPMKIIIPKNTIFSAEKPSPTGWYYEG
                     VSQASELAWKALAPLAPNRFSAGTYMSLCATYICGNNVKNQYVHIEPQHGGWGATINR
                     DGANALIAITDGDTYNYSIELLEAKFPLRLVQYALNTK"
BASE COUNT          422 a          231 c          204 g          474 t
ORIGIN      
        1 tctttggtat ttaatgcgta ttgcaccaag cgtaagggaa atttagcttc tagaagttct
       61 attgaataat tgtaagtatc tccatctgtt atcgcaatca atgcatttgc accatctcta
      121 tttattgtag ccccccagcc cccatgttgg ggttcaatat gtacatattg gttttttaca
      181 ttattcccgc aaatataagt tgcgcataga ctcatataag ttccagcaga aaatctattt
      241 ggagctaatg gtgctaaggc tttccatgct aattctgaag cttgtgatac accttcataa
      301 taccagccag ttggagatgg tttttcagca ctgaaaattg tatttttagg aataataatt
      361 ttcataggtt taaaataccc ttcgttggat ggagcaaacg gctcaacaag agctttaaag
      421 acagtcttta ctgctgaagt taatgcacct aaagagcagt ttattggagc ttttcttgct
      481 tgaggacagc cagtataatc aaagagtata tttgaacctt taatttgtat ttttatttga
      541 acagaaattt gatttttaac attaccatct ccatcgatat aatcttttgc tttgtagata
      601 ccatctggaa gtttagaaat tattcttcta ctttgctttt cactatctaa aagaatttga
      661 ttaaaagctt tattaagagt tgtctcgcta tatttcctat atgtttcttt tattctttta
      721 tcagcgattt taactgctgc aatttgagca tttaaatcac ccattgctag ctttgggaat
      781 cttacatttt ctaaaataat agaggcaatg tcaggcaata atttattatc tctacataat
      841 ctaaccccag aaaagcgtat tccttcttta aacatagagt cagaatctgg aggcagactt
      901 ccagcaattg agcctcccac atcaagccag tgagctacag aaatagaaaa agcaatattt
      961 tttttcttaa aaaaaatagg tttaattata gcaaaatcac atggatgtgt ccccccttta
     1021 aaaggatcat tagttaaaaa aatgtctcca tctactgtat ttttgctaaa cttatttatt
     1081 atgaatttaa cctgtgttga aaaggtacca gtaaacaatg tgacaccatt agtttgggaa
     1141 actaaattac ctttgttatc gcaaattcca caagcaaaat ctaaaacttc atatattaca
     1201 gggctcatgc tagttcttgc aagcacaaca gccatttcat cagctgttga aaggagtttc
     1261 cctttaatta tttctaatgt tattgaatcc ataaaaattt tttattaaag ttgtttatag
     1321 tttaataaaa a
//
LOCUS       DTVV01000537            1529 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_943821, whole genome shotgun sequence.
ACCESSION   DTVV01000537 DTVV01000000
VERSION     DTVV01000537.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_943821"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1529)
                     /locus_tag="EYO86_06405"
     CDS             complement(<1..>1529)
                     /locus_tag="EYO86_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIC42699.1"
                     /translation="SALLKNNYEVSLFEAGYSHHNFLLDVPAGFFKLVNDSKYATYHK
                     TAPQAHLGGRENIVPQGNVLGGGTSINAQVYMRGRPQDYNEWHEILRVNNDTVGWAWS
                     DLLPYFKNMENNNRLENEYHGKSGPLKVSDSSHINKLTDDFIKTVQELGVPLTDDFNG
                     KAQHGVGLYQFMNRKGKRSSAAYAFIEPEINNSNLHLQLNTRVEKIIIKDNKAIGVEY
                     RNNKGELNKKFANKEVVVAAGSFITPKLLMLSGIGNADELKLHDIGCTADLPGVGENL
                     IDHPECPIIAKANGAYGYYKQGKGWRMIKNGLQFLLFGTGPVNSTGVEAGAFINPLSF
                     EDLPSIQAFFVPSIYMNSDTIGLIDDDYGMSITTVVTKPLSRGKVKLKSANPIERPII
                     ELNLLKHEKDLETMMAGQRFFLNTFKQGELGKKVKEILIPDVRDLDDAKLEAHCRKFV
                     RTNYHPSGTAKMGADSDKMAVLDSKMRVRGIENLRVCDLSVLPNINSGNTAASAMMLG
                     L"
BASE COUNT          458 a          323 c          221 g          527 t
ORIGIN      
        1 cttaaaccta acatcatagc tgaagcagca gtattgcctg agttgatatt aggcagaacc
       61 gataaatcgc atacacgcaa gttttcaatg cctcgaaccc tcattttgga atctaaaacc
      121 gccattttgt cactatctgc tcccattttt gctgttcctg aaggatgata gttcgttcgt
      181 acaaattttc tgcaatgcgc ttctagttta gcatcatcta aatcccttac atcaggaatt
      241 aaaatttctt ttactttttt accaagctct ccctgcttaa aagtattaag aaaaaacctt
      301 tgtccagcca tcatagtttc taaatccttt tcatgtttaa gtaaattcag ttcaattata
      361 ggcctttcaa taggattagc tgattttagt tttacttttc cccttgataa tggttttgta
      421 accactgttg taattgacat tccataatca tcatcaataa gcccaatagt atctgaattc
      481 atataaatac ttggaacaaa aaaagcttga attgaaggta aatcttcaaa acttaaggga
      541 tttatgaatg ccccagcttc aacaccggta gaattgacag gacctgtacc aaataacaaa
      601 aactgtaaac cattttttat cattctccat ccttttccct gtttataata accataagct
      661 ccatttgctt ttgcaatgat agggcattct ggatgatcaa ttaaattttc acccacacct
      721 ggaagatcag ctgtgcaacc aatatcatgc aattttaatt catcagcgtt cccaataccg
      781 gataacatca ataacttcgg agtgatgaat gatcctgcgg caacaacaac ttctttattg
      841 gcaaactttt tattcagctc ccctttatta tttctatact caacgcctat tgctttatta
      901 tctttaatga tgattttttc gactcttgta tttaattgta aatgtaggtt agaattattt
      961 atttcgggtt caataaaagc ataagccgcc gaacttcttt tccccttgcg attcataaat
     1021 tgataaagac ctaccccatg ttgtgctttc ccattaaaat catctgttaa tggaacccct
     1081 aactcttgaa cggttttaat aaaatcatcc gttaacttat taatatgtga tgagtcagac
     1141 acttttaatg ggcctgactt tccgtggtac tcgttttcca accgattatt attttccata
     1201 tttttaaaat atggcagcaa atcactccat gcccatccca ctgtatcatt gttaactcgt
     1261 aaaatttcat gccattcatt gtagtcctga gggcgtccgc gcatataaac ttgggcatta
     1321 attgatgtac cacctcccaa tacattaccc tgtggcacaa tattttcacg tccccccaaa
     1381 tgtgcttgtg gggctgtttt atgataagtg gcatattttg agtcattaac gagtttaaaa
     1441 aatccagcag gtacatctaa aagaaaatta tgatgggagt atcccgcttc aaataaactt
     1501 acctcataat tattttttaa taatgctga
//
LOCUS       DTVV01000538            1056 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_950372, whole genome shotgun sequence.
ACCESSION   DTVV01000538 DTVV01000000
VERSION     DTVV01000538.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_950372"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            99..1019
                     /gene="ybgF"
                     /locus_tag="EYO86_06410"
     CDS             99..1019
                     /gene="ybgF"
                     /locus_tag="EYO86_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIC42700.1"
                     /translation="MFRYFVFILILLFVGPSYSEDSFLTLKQQLDRLQREVSDLSKSV
                     FAGSRDAQSEQKKQSETSPNLTALTAFDLRIYDLEKDIKRLNKDFEELIFQIDDLKIL
                     MDELTLSLDTKFTNNQELSELDQTTTQIEINKEESNLIVNENSLGDLVTSSENLSDEN
                     DGLVLKSDDKSNINIKQSPADEFQQAIGLIMSQEYDDAIISLKKFIDNNKERNLAGSA
                     HYWLGEIYIFRKEYREAAIVLLEGYQKYPNSVKAPDMLYQLSESLINIEKNTEACNML
                     KTLSTEYPEYKLLDKSQARISELGCIIAVE"
BASE COUNT          413 a          128 c          166 g          349 t
ORIGIN      
        1 taaagttgac taatataaat tctttatact tattcaggcg ctgacttatt tcaagttagc
       61 gccatatttt tgtcctaaaa ttaatataag cgaataacgt gtttagatat tttgttttta
      121 ttttaatttt gctttttgta ggtccatcat actcagaaga ttctttttta actttaaaac
      181 aacaattgga taggttgcaa agggaagtta gtgatttatc caaatcggtt tttgcaggtt
      241 ctcgagacgc ccaaagtgaa caaaaaaaac aatcagaaac atctcctaat ctaactgctc
      301 taactgcgtt tgatttaaga atttatgatt tagaaaaaga tattaaaaga ttgaataaag
      361 attttgaaga gttaattttt caaattgatg atttgaagat tctaatggat gaattaactt
      421 taagtttgga tacaaaattt acaaacaatc aagaattatc tgaattagat cagacaacaa
      481 cccaaataga gataaataaa gaagaaagta atttaatagt gaatgaaaat agtttaggcg
      541 atcttgtgac aagttcagaa aatctttctg atgaaaatga tggattagtt ttaaaaagcg
      601 atgataaatc gaatataaac attaagcaaa gccctgcaga tgaatttcag caagctattg
      661 gtttaataat gtcacaagaa tatgatgatg caataatttc cttaaaaaaa tttattgata
      721 ataataaaga aagaaatctt gcgggttccg ctcattattg gttaggggaa atttatatat
      781 ttagaaagga gtatcgtgaa gctgcaatag ttttgttaga aggatatcaa aaatatccca
      841 atagtgtaaa agcaccagac atgttgtatc aactttcaga atctttgatt aatattgaaa
      901 aaaatacaga agcttgcaat atgcttaaaa cattatcaac tgaatatcca gaatataaac
      961 tactagataa atcccaagct agaatttcag aattaggatg tatcattgct gtagagtgaa
     1021 aattacacaa tcttatttta atctaatttt aaaaaa
//
LOCUS       DTVV01000539            2609 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_966341, whole genome shotgun sequence.
ACCESSION   DTVV01000539 DTVV01000000
VERSION     DTVV01000539.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_966341"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="EYO86_06415"
     CDS             complement(<1..363)
                     /locus_tag="EYO86_06415"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:PF00749.19"
                     /note="charges one glutamine molecule and pairs it to its
                     corresponding RNA trinucleotide during protein
                     translation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIC42701.1"
                     /translation="MISKARFAPSPTGLLHVGNARSAVLNWAYINNKGGEFILRIDDT
                     DKQRSKTEFEYQIKEDLKWLGITWHKTFNQSGRQNIYVKKIQELKNLNKIYPCFESPE
                     ELSLKKKSQLLSGKPPIYD"
     gene            complement(364..1104)
                     /gene="nadE"
                     /locus_tag="EYO86_06420"
     CDS             complement(364..1104)
                     /gene="nadE"
                     /locus_tag="EYO86_06420"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIC42702.1"
                     /translation="MKTNERVNCILNWMKQYCHSVKYQPVTLVIGVSGGVDSAVTSTL
                     AANTGFNTIAVSMPIMQNSEQHDLSLRHLEWLKSNFNNVITKVVNLDDIFSSFKKTMH
                     EFDNNLAFANSRSRLRMVILYQIAQSNKGLVVGTGNKVEDFGVGFFTKYGDGGVDISP
                     LADCTKTQVWEMASEMSIIQDIIDAQPTDGLWEDNRNDERQLGMTYKQIEEAMINKES
                     KYYDKYMQIRKLNLHKMKPIPVCEFEDD"
     gene            complement(1127..2503)
                     /locus_tag="EYO86_06425"
     CDS             complement(1127..2503)
                     /locus_tag="EYO86_06425"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HIC42703.1"
                     /translation="MTRNWKPNSWRDKPARHIPTYSNDEELSVAREKLSNFPPLVFAG
                     ECRNLLKQLEEVAKGRAFLLQGGDCAESFSDFHPNNIRDLFKVILQMAVVLTFGASCP
                     VVKVGRMAGQFAKPRSQDTEIINNVELESYKGDIINGIDFDKTSRTPNPKRLIQAYNQ
                     SAATLNLLRAFAQGGFANLKQIHQWNLSFVEDSQSKKKFEEMANTIDECLTFMEACGI
                     NDQNVRQMKETDFYTSHEALLLPYEESLTRIDSTTGQWYDVSAHMLWVGDRTRQLDGA
                     HIEFVRGIQNPIGLKVGPTTDVVELLKIIEVINPDNITGRISLICRMGADKITSLLPN
                     IISQVKKDEKNVVWVCDPMHGNTVKASSGYKTRPLINIISEIEKFFKIHQNEGTYPGG
                     IHLEMTGQDVTECIGGVQEITEDDLKSRYHTYCDPRLNASQSLEVAFLLSEFLKDDRI
                     RLQQSSKK"
BASE COUNT          791 a          502 c          389 g          927 t
ORIGIN      
        1 atcataaatg ggcggtttac ccgataagag ttgagatttt tttttgagag ataattcttc
       61 agggctctca aaacaaggat agattttgtt gagattcttt agttcttgaa tttttttaac
      121 atagatgttt tgtctacctg attgattaaa cgttttatgc catgtaattc ctaaccactt
      181 taaatcttct ttgatttggt attcaaattc tgttttactt ctttgcttgt cagtatcatc
      241 aatacgtaag ataaattctc cacccttgtt atttatgtac gcccaattta gaacagcaga
      301 tctcgcatta ccaacatgca ataaccctgt tggacttggg gcaaaacgag ctttagatat
      361 caattaatca tcctcaaatt cacaaactgg tattggtttc attttatgta aatttagttt
      421 tctaatttgc atatacttat cataatattt actttcttta ttgatcatag cctcttcaat
      481 ttgcttgtat gtcattccga gctgtctttc gtcattacga ttatcttccc ataagccatc
      541 tgtcggttga gcatctatta tatcttgaat aattgacatt tcagaagcca tttcccaaac
      601 ttgtgttttt gtacaatctg cgagagggga aatatcaaca cctccatctc catactttgt
      661 gaagaagcca acaccaaaat cctctacttt atttcctgtt cctacaacta accctttatt
      721 agattgcgct atttgataca aaattaccat tcttaaacgt gatcgagaat ttgcaaatgc
      781 aagattgtta tcaaactcat gcatggtttt cttaaaggaa gaaaaaatat catctaaatt
      841 aaccactttt gtaatcacat tgttaaaatt tgatttaagc cattctaagt ggcgtaaact
      901 taaatcatgt tgctcggaat tctgcataat gggcattgaa acagcaattg tgttaaatcc
      961 ggtatttgcc gctagagtac tggtaacggc agaatcaact cctcccgaaa ctcctataac
     1021 aagagtgact ggttgatatt taactgaatg acaatattgt ttcatccaat taagaataca
     1081 atttaccctt tcattcgtct tcataaatat ttgtatagca gtaaaattat tttttggaag
     1141 actgctgtaa tcttattcta tcatctttta aaaactcaga tagtaaaaaa gctacttcta
     1201 aagactgaga ggcatttaag cgtggatcac aataagtatg atatctgctt ttcaaatcat
     1261 cctcggttat ttcttgcact ccaccaatgc attcagtcac atcttggcca gtcatttcca
     1321 agtgaatacc tccagggtaa gtaccttcat tttgatgaat cttaaaaaat ttctcaattt
     1381 cagaaataat attgatcaat ggtcgcgttt tatatccaga agatgcttta acagtatttc
     1441 catgcatagg atcgcaaacc catacaacat ttttttcatc tttttttact tgcgatataa
     1501 tattaggcaa aagagatgtt atcttatcag cacccattct acaaattaac gaaattctcc
     1561 ccgtgatatt atctgggttt attacttcaa taattttcaa gagttccacg acgtcagtcg
     1621 ttggacctac ctttaaaccg attggatttt gaattcctct cacgaattct atgtgagctc
     1681 catccaattg tctcgttctg tctcctaccc aaagcatatg agctgaaaca tcgtaccact
     1741 gacctgttgt actgtctatg cgagtaaggg attcctcata tgggagtaat aaggcttcat
     1801 gacttgtata aaaatccgtt tctttcattt gacgaacatt ctgatcatta attccgcaag
     1861 cttccataaa agttaggcat tcatcaattg tattggccat ttcttcaaat tttttcttcg
     1921 attgactatc ttcaacaaaa cttaaattcc attgatgtat ttgttttaaa tttgcaaaac
     1981 ctccttgagc aaatgcacgg agcaaattta aggtagctgc ggattgatta taagcttgaa
     2041 ttagcctttt ggggtttggc gttcttgaag ttttatcaaa atcaattcca ttaataatat
     2101 ctcccttgta agattctaat tccacgttat taattatctc agtatcttgt gatctcggtt
     2161 tggcaaattg acctgccata cgaccaactt taacaacagg acaggaggcg ccaaaagtaa
     2221 ggactacagc catttgaagt atcactttaa ataggtctct aatgttatta gggtgaaaat
     2281 cagaaaaact ttcggcacaa tcgcctccct gtaaaagaaa ggctctccct tttgcaactt
     2341 cctctaattg ttttaataaa tttctacact ccccagcaaa aactaatgga ggaaaattag
     2401 ataatttttc cctagccaca cttaactctt catcatttga ataagtaggt atatgtcttg
     2461 caggtttatc tctccaagaa ttaggtttcc aattgcgagt cataataaaa aatgtctaat
     2521 aaactctctc gaatcttgaa acaaggacca atatagttat ttttttctat tttttttgag
     2581 aatttttaat tttgaacgct ctaaagcta
//
LOCUS       DTVV01000540            1483 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_966377, whole genome shotgun sequence.
ACCESSION   DTVV01000540 DTVV01000000
VERSION     DTVV01000540.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_966377"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="hslU"
                     /locus_tag="EYO86_06430"
     CDS             complement(<1..1045)
                     /gene="hslU"
                     /locus_tag="EYO86_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIC42704.1"
                     /translation="MESSFSPREIVSELDKFIIGQQKAKRAVAVALRNRWRRKQLPES
                     LREEILPKNILMVGPTGCGKTEISRRLAKLAHAPFIKVEATKFTEIGYVGRDVEQIIR
                     DLVEISISQAKIKMGKEVQAHAEKNAEERILDVLVSKTSSPATREAFRKKLRNGELNN
                     NEVEIPVAINSSFNLPTMDIPGMPGSQMGMINLGDVLGKSFSAPKKNKKMTIEESHTY
                     LLQEETDRLLDNDRIINKAIEEVEQNGIVFIDEIDKITSRHDKASGDVSREGVQRDLL
                     PLIEGTTVSTKHGAVKTDYILFIASGAFHLSKPSDMLPELQGRLPIRVELDSLTKEDF
                     KLILTEPQNSLIKQ"
     gene            complement(1048..>1483)
                     /gene="hslV"
                     /locus_tag="EYO86_06435"
     CDS             complement(1048..>1483)
                     /gene="hslV"
                     /locus_tag="EYO86_06435"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010300334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIC42705.1"
                     /translation="VKKVRRLGSDNLVIAGFAGSTADAFALFERLESKLEQYKNQLKR
                     SCVELAKDWRTDRYLRRLEAMMIVADRDISLLLSGTGDVIESDDGVLAIGSGGPYANS
                     AAQALIKNTNMNAKEIAVESLNIAADICVYTNHNLVTEEIKI"
BASE COUNT          407 a          314 c          219 g          543 t
ORIGIN      
        1 attgtttgat caaactattc tggggctcag taaggattaa tttaaaatct tctttagtta
       61 agctatcaag ctcaactcgg ataggtaacc gtccctgtag ttcaggcaac atatctgatg
      121 gcttagatag atgaaatgca cccgaagcaa taaacaaaat gtaatcagtt tttactgccc
      181 cgtgtttggt gctaacagtt gtaccttcaa ttaatggcaa aagatcgcgc tgaaccccct
      241 ctctcgacac gtctccacta gccttatcat gtcttgatgt aattttatct atttcatcaa
      301 taaatactat tccattttgc tctacttctt ctatagcttt atttattatc ctatcattat
      361 ctaaaagcct atcagtttct tcttgaagta agtaagtgtg agattcctca attgtcattt
      421 ttttattttt ttttggtgca gaaaatgatt ttcctaaaac atcacctaag ttgatcattc
      481 ccatttgaga ccctggcatt cctggtatat ccattgtagg aaggttgaaa gaagaattga
      541 ttgcaacagg aatttcaact tcattattat ttagttcgcc attgcgtagt ttttttctaa
      601 aggcctctct agtagctgga ctagatgttt tggaaaccaa cacatctaat attctttcct
      661 cagcattttt ttcagcatgg gcttgaactt ctttacccat tttgatcttt gcttgactta
      721 tgcttatttc aacaaggtct ctgatgattt gttcaacatc tcgccccaca taacctattt
      781 ctgtgaactt tgttgcctca actttaataa agggagcatg tgcaagtttt gctaaacgac
      841 gtgagatttc tgtttttcca catcccgtgg gcccgaccat taaaatattt tttggaagta
      901 tttcctctct taaactttct ggcagctgtt ttcttctcca tctgttgcgc aatgctactg
      961 ctacagctcg tttagctttt tgttgaccaa tgataaattt atctaattca gaaactatct
     1021 ctcttggact aaaactagat tccataatta tatttttatt tcttctgtta ctaaattatg
     1081 attagtataa acacatatat ctgctgcaat attaagtgat tcaactgcaa tttctttagc
     1141 attcatattt gtatttttta taagggcttg ggccgcacta tttgcgtaag gaccacctga
     1201 tccgatagcc aatacaccat catcagattc aattacatcc ccagttcccg atagtaacaa
     1261 actaatatcc ctatctgcta caatcatcat ggcctctaat cttcttaaat agcggtcagt
     1321 tctccaatct ttagctagtt caacgcaaga tctctttaat tgatttttat attgctccag
     1381 ctttgattcc aatctctcaa aaagagcaaa tgcatctgca gtagatcctg caaacccagc
     1441 tataactaaa ttatccgatc caagtcgtct tactttttta acg
//
LOCUS       DTVV01000541            1300 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_969432, whole genome shotgun sequence.
ACCESSION   DTVV01000541 DTVV01000000
VERSION     DTVV01000541.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_969432"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1300)
                     /gene="purL"
                     /locus_tag="EYO86_06440"
     CDS             complement(<1..>1300)
                     /gene="purL"
                     /locus_tag="EYO86_06440"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIC42706.1"
                     /translation="KTKNLLNGVVSGIGGYGNCMGIPTVGGEVKFDSSYNENILVNAM
                     AVGLVKKNKIFYSKAKGTDQPVIYVGSKTGRDGIHGASMASAEFDKNAEAKKPTVQVG
                     DPFTEKLLLEACLELMKDDSIISIQDMGAAGLTSSSVEMASKGNLGIEINLNKIPCRE
                     DKMTPYEMMLSESQERMLLILNSEKVENAKKIFIKWGLDFSVIGKTTNTKNLVLNFDG
                     EEVANLPVNSLSNNVPIYDRKWKKNVTSIKKNLQIDFKSFKILESLKKILMSPNNAEK
                     SWVWEQYDHTVMGDTIQKPGGDSAVIRIHGKNKGIAITVDSSAHYCLANPVAGGKQIV
                     SEAWRNLISVGASPIAITNCLNFGNPEKDKIMGQFVECIDGISQACTYLDFPVVSGNV
                     SFYNETQNKAILPTPTIGGVGLLKNLNTMMTKNIKEVGSY"
BASE COUNT          396 a          247 c          173 g          484 t
ORIGIN      
        1 atataacttc ctacttcttt tatatttttt gtcatcatgg tatttaaatt ttttaataaa
       61 cctacaccac caatggtggg tgtgggcaat atcgccttgt tttgtgtttc gttataaaaa
      121 gaaacatttc cagaaaccac tggaaagtct aaataagtac aagcttgaga aataccatca
      181 atacattcga caaactgacc cattatttta tctttttccg gatttccaaa atttaaacaa
      241 ttagtaattg ctattggact tgcacctact gaaataagat ttctccaggc ttcagaaaca
      301 atctgtttac ctccagctac agggtttgct aaacaataat gggcagagga gtccaccgta
      361 attgctattc ctttattttt tccatgaatt ctaattacag cagaatcgcc acctggcttt
      421 tgaattgtat ctcccatgac agtatgatcg tactgttccc aaacccaact tttttcagca
      481 ttgtttgggc tcattagtat ttttttcaaa gattctagta ttttgaatga tttaaaatct
      541 atttgtaaat tttttttaat agaagtaaca ttttttttcc attttctatc gtatattgga
      601 acattgttag ataatgaatt tacaggtaaa tttgcaactt cttcaccatc aaaatttaaa
      661 actaaatttt tagtgttagt agtttttcct attacagaaa aatccaatcc ccatttgata
      721 aaaatttttt ttgcattttc tactttttca gaatttaata tcaaaagcat tctttcttgg
      781 ctttcagaaa gcatcatttc gtatggagtc attttatctt ctctgcaagg gattttgttt
      841 aaatttattt caattcctaa attacccttc gaagccattt caacacttga agaagttaat
      901 cctgcagcac ccatatcctg tatagaaata atagaatcat ctttcataag ttctaagcaa
      961 gcctccagaa gaagtttttc agtaaaaggg tcaccgactt gtacagttgg tttttttgct
     1021 tctgcatttt tatcgaactc agccgaagcc atactagcac catgtattcc gtctcttcca
     1081 gttttagatc caacataaat cactggctga tcagtacctt ttgcttttga gtaaaatatt
     1141 ttattttttt ttactaaacc aactgccata gcattaacta aaatattttc attataggaa
     1201 gaatcaaatt ttacttctcc cccaacggta gggataccca tacaattacc ataacctccg
     1261 attccagaga caacaccatt taaaaggttt ttggtttttg
//
LOCUS       DTVV01000542            1782 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_973340, whole genome shotgun sequence.
ACCESSION   DTVV01000542 DTVV01000000
VERSION     DTVV01000542.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_973340"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /gene="thrS"
                     /locus_tag="EYO86_06445"
     CDS             <1..1205
                     /gene="thrS"
                     /locus_tag="EYO86_06445"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017971742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIC42707.1"
                     /translation="AEKRDHRKLGKKMSLFHFQEEAPGAVFWHAKGWKLFQSLINYMR
                     KRQDQSGYVETNTPDLLDKSLWEASGHWEKFGESMFTTEAKEEKVFAIKPMNCPGAVE
                     VYKQGLKSYRDLPLRMAEFGKVHRYEPSGALHGLMRLRAFTQDDAHIFCTEEQITNES
                     KKVCDLILSIYKDFSFTDVRIKYSDRPEKRMGDDKTWDKAEAALKNAMEATKLDYTFN
                     PGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDLNLPGRLGATYVDENGQKKVPVMLHRA
                     LFGSLERFAGILIEHYAGHLPFWLSPTQVVITTITSDTDEYAKEVKAALQKLGINSEV
                     DIRNEKIGYKIREHSNSKIPIILAVGKKELQDKTVSVRRLGKTSTEVINLEEIKLILK
                     KESLSPIE"
     gene            1205..1687
                     /gene="hisI"
                     /locus_tag="EYO86_06450"
     CDS             1205..1687
                     /gene="hisI"
                     /locus_tag="EYO86_06450"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIC42708.1"
                     /translation="MDNIIFEKRKSVEQVEESTELAPKFDQNGIIPVVTTDYDTGEVL
                     IQGYMNEEAFKRTIKLGEAVYYSRSRNKIWHKGQTSGLIQKVKEIRIDDDQDCVWLRV
                     NVIGGASCHVGYRSCFYRSIPFGKNNKSSIIKLKFEEKEKVFDPKKLYRNSSGRKLCI
                     "
BASE COUNT          682 a          229 c          351 g          520 t
ORIGIN      
        1 aagcagaaaa acgtgatcat cgaaaactcg gaaaaaaaat gagtttattt cattttcaag
       61 aagaagctcc tggagctgtt ttttggcatg ctaaaggatg gaaattattt caatcactaa
      121 ttaattatat gcgtaaacga caagatcaat ctggttatgt agaaactaat acgcctgact
      181 tattagataa atctttatgg gaggcctcag gacattggga aaagtttggt gagagtatgt
      241 ttacaacaga agctaaagaa gaaaaggttt ttgctataaa accaatgaat tgccctggtg
      301 cagttgaggt atataaacaa ggtcttaaaa gttatagaga tttaccatta cgcatggcag
      361 aatttgggaa ggtacatcgc tatgaacctt ctggagcctt acatggactt atgcgcctgc
      421 gtgcttttac acaagacgat gcacatatat tttgtacgga agaacaaatt acaaatgaga
      481 gtaaaaaagt atgtgattta atattaagta tttacaaaga ttttagtttt acagatgttc
      541 gaataaaata ttctgatcgt ccagaaaaaa gaatgggaga tgataaaact tgggacaaag
      601 cagaagctgc tttaaaaaat gcaatggaag caacaaaatt ggattatact tttaatcctg
      661 gagaaggggc tttttacggt ccaaagttag aatttgttct tagagatgca atagggagag
      721 actggcaatg tggaacatta caagttgatc tcaatctacc aggaagatta ggagcaactt
      781 atgttgatga aaatggacaa aaaaaagttc cagttatgtt gcatagggcc ttatttggtt
      841 ctttagaaag atttgcaggt atcttaatag aacattatgc aggtcattta ccattttggc
      901 tttcgccaac tcaagtagtt attactacaa taacatctga tactgatgaa tatgcaaaag
      961 aagttaaggc agcattacaa aaattaggaa taaatagtga agtagatatt agaaatgaaa
     1021 aaattggtta taaaattaga gagcatagca attctaaaat tcctataata ttagctgttg
     1081 gaaaaaaaga attgcaagat aaaacagttt cagttcgacg ccttggaaaa actagtactg
     1141 aggtgataaa tttagaagaa ataaaattga ttttaaaaaa agaatcacta tcacctatag
     1201 agtaatggat aatattattt ttgaaaaaag aaaatcagtt gaacaagttg aggaatcaac
     1261 agaattagct ccgaaatttg accaaaatgg aatcattcct gtcgtaacta ctgactatga
     1321 tacaggtgag gttttaattc aaggttatat gaatgaagaa gctttcaaaa gaacaattaa
     1381 acttggggaa gcagtttact attctagaag tagaaacaaa atttggcata aaggtcaaac
     1441 tagtggttta attcaaaaag taaaagaaat tcgcattgat gatgatcagg attgtgtatg
     1501 gttgcgagtt aatgttattg gaggtgccag ttgtcatgtt ggttatcgct catgttttta
     1561 ccgaagcatt ccatttggta aaaataataa atcttcaata ataaaactca aatttgaaga
     1621 aaaagaaaaa gtttttgatc caaaaaaatt atataggaat tcatctggaa gaaaattatg
     1681 tatataaaat aaaagccccg atcaaaaaaa gatcggggct tttaaggagt taaaactaga
     1741 tattacttac gacctctggc catttttgcc cgagaaacgt ca
//
LOCUS       DTVV01000543            1588 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_978952, whole genome shotgun sequence.
ACCESSION   DTVV01000543 DTVV01000000
VERSION     DTVV01000543.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_978952"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..83)
                     /locus_tag="EYO86_06455"
     CDS             complement(<1..83)
                     /locus_tag="EYO86_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42709.1"
                     /translation="MKIAREPVSFETPVGEDDDSFLGDFIE"
     gene            complement(133..585)
                     /locus_tag="EYO86_06460"
     CDS             complement(133..585)
                     /locus_tag="EYO86_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC42710.1"
                     /translation="MALHFSKTEFQDREKSVIYSLREKNLDALLIFRQESMYWLTGYD
                     TFGYVFFQCLVLTARGEKILLTRTPDLRQAQNTSIIKDIRIWIDKDGASPSNELKNIL
                     YELNLGKKNIGVEYESYGLTGRNALRLNQSLVDFSLKLKNKLSFFMYF"
     gene            complement(587..1543)
                     /locus_tag="EYO86_06465"
     CDS             complement(587..1543)
                     /locus_tag="EYO86_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HIC42711.1"
                     /translation="MNKNYFEIMRGGVHTTFQDSGYSNVQHLGITTGGVVDNDLFRLA
                     NEIVNNELLTPILEFANQGPQLKLKKGKCRFTITGDVAFNIWCDGSIVEGIPYRSYLL
                     NEGDTLDILSTIKSNYGYLAVEGGFVVTQHYGCSSTLTQSQIGPNEGKKISDNQLLYF
                     HQDGSRLISSLDFNQYKYQDDAIRVLRGPQMNYFMQKVIKNFYSKPFTISNTTNRMGV
                     RIEGNKISSIKSHNIASEGIIKGSIQVPGNGNPIILMAEHPSIGGYPKIATVILADIA
                     RIAQFTIGTKFNFKEVDLTDAENIFRGKNKFFESLLNKIKCN"
BASE COUNT          499 a          292 c          209 g          588 t
ORIGIN      
        1 tcctctataa aatctcccaa gaaactatca tcatcctctc ctacaggtgt ttcaaaacta
       61 acaggttctc gtgcaatttt cataactttt cttatcgacg ggaaagttcc gcaaaaagct
      121 tttattgggg ttttaaaaat acataaagaa acttagtttg ttttttaatt tcaatgaaaa
      181 atccacaaga gattggttaa gccgcagggc gttcctccct gtcagaccat atgattcata
      241 ctccacgcca atattctttt tgccaagatt caattcatac aaaatatttt ttagttcatt
      301 gcttggacta gcaccatctt tatcaatcca tatccgtata tctttaatga tggaagtatt
      361 ttgtgcttgt cgcaaatcag gggttcttgt taataatatt ttttcgcccc tggcagttaa
      421 aacaaggcat tgaaaaaaaa cataaccaaa tgtgtcatag cctgttagcc aatacatcga
      481 ttcttgccta aaaattaata acgcatctaa gtttttttct cttagggagt aaattactga
      541 tttttcccga tcttgaaatt ccgtttttga aaaatgtagt gccatgtcag ttacatttta
      601 ttttatttaa caaagattca aaaaatttat ttttccccct aaatatattc tctgcatctg
      661 tcaaatctac ttctttaaaa ttaaattttg tacctattgt aaattgagca attcttgcaa
      721 tgtccgccaa aatcactgta gcaattttag gatatcctcc aatcgaagga tgttccgcca
      781 taagaataat aggatttcca tttccaggta cctgaataga tccttttata attccctcag
      841 aggctatatt atgtgatttt attgatgaaa ttttattacc ttcaattctt actcccatcc
      901 tattggttgt attgctgata gtaaaaggtt ttgaataaaa atttttaatc actttttgca
      961 taaaataatt catttgcgga cctcttaaca ccctgattgc atcatcttga tatttgtatt
     1021 gattaaaatc caaagaacta attaatcttg atccatcctg atgaaaataa agcaattggt
     1081 tatcagaaat ctttttaccc tcattagggc ctatttgtga ttgtgttaaa gttgaagaac
     1141 atccataatg ttgagttact acaaatcctc cttcgactgc taaatatcca taatttgatt
     1201 taatggtaga caatatatcg agtgtatcac cctcatttaa taaatatgaa cgataaggaa
     1261 ttccttcaac aatactacca tcacaccata tattaaaggc aacatctcct gttattgtaa
     1321 aacgacattt tcctttttta agttttagct gcggtccttg attcgcaaac tctaatatgg
     1381 gggtaagcaa ttcattattt acaatttcat ttgctaatct aaataagtca ttatcaacaa
     1441 ctccacctgt agttattcct aaatgttgaa catttgagta accactatct tggaatgttg
     1501 tatgtacgcc acctctcatt atttcaaaat aatttttatt cattattttt attcattttt
     1561 ccaaattgtt ttttggaaat tgatttaa
//
LOCUS       DTVV01000544            1718 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_981330, whole genome shotgun sequence.
ACCESSION   DTVV01000544 DTVV01000000
VERSION     DTVV01000544.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_981330"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="EYO86_06470"
     CDS             complement(<1..195)
                     /locus_tag="EYO86_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIC42712.1"
                     /translation="MTYKSDFLNEIESRGFIYQSSEIEDLDNLMFKKNITAYIGFDIT
                     SDSLHIGSLVQLMLLHWLDYF"
     gene            complement(230..877)
                     /locus_tag="EYO86_06475"
     CDS             complement(230..877)
                     /locus_tag="EYO86_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIC42713.1"
                     /translation="MVDLLISGPEGKIEAKYSHNNAHNSPIVLILHPDPSRGGTMNTK
                     IIFRLYKLFVDNGFSAIRFNFRGVGKSEGLFDDGEGELSDAASVLDWLQQYNTNSKVV
                     WVAGFSFGAWVAMQLLMRRPEINNFISVSPPANTRDFSFLAPCPSTGLIVQGDKDTIT
                     SFDSTKILSEKLQKQKKVNIHFKSIKGADHFYENFTDQFSSTIDNYIKDSLANLK"
     gene            935..>1718
                     /gene="mnmA"
                     /locus_tag="EYO86_06480"
     CDS             935..>1718
                     /gene="mnmA"
                     /locus_tag="EYO86_06480"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:PRK00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIC42714.1"
                     /translation="MNSLGFNKQNKDTKVVIAMSGGVDSSVAATMLKKEGYDIIGITM
                     KLYSQTKAKSSKSCCAGRDIEDAKKVAEQHDFPHITLDYQDKFFSGVIDNFIETYAEG
                     QTPVPCIQCNQTVKFRDLLNEAKAMNADTLVTGHYVRRLEDKSNVKLFKAKDASKDQS
                     YFLFATLKDQLDYLRFPLGNYLKSEVRKMAENFKLEVSDKPDSQDICFVTSSSYRDLI
                     NKLKPELNIEGNFLDSEGNILGKHKGIANYTIGQRRGLGVGGS"
BASE COUNT          630 a          265 c          284 g          539 t
ORIGIN      
        1 gaagtaatca agccaatgaa gcagcattaa ttgcactaaa cttcctatat gtaaactatc
       61 gcttgtaata tcaaaaccga tataagcagt aatgtttttt ttaaacatga gattgtctag
      121 atcctcaatc tcagaagatt gataaataaa acctcgagat tctatttcgt ttaaaaaatc
      181 tgatttatag gtcataaaaa taaaaattaa gttttattta tggttaattt cactttaggt
      241 tggctaaact atctttaata tagttatcta ttgtagatga aaattggtct gtaaagtttt
      301 cataaaaatg atcagcgcct tttatgcttt taaaatgtat gttaactttt ttttgtttct
      361 gtaatttctc agataagatc tttgtagaat caaaggaagt tatagtatct ttgtcaccct
      421 gaacaattaa acctgtggaa gggcaaggcg ctaaaaaact aaaatcacgg gtatttgctg
      481 gcggagaaac actaataaag ttattaattt ctggtcttct catcaaaagt tgcatcgcaa
      541 cccatgcccc aaaagaaaaa cctgcaaccc aaacaacttt agaattagta ttgtattgct
      601 gtaaccaatc caaaacactg gcagcatcgc ttaactctcc ctctccatca tcaaacaaac
      661 cctcactttt gccaacacct ctaaaattaa atctaattgc agaaaaacca ttgtctacaa
      721 aaagtttata taaacgaaaa ataatttttg tattcattgt acctccccta gaggggtcag
      781 gatgtaaaat gagtactatt ggcgaattat gggcattatt atgactatat tttgcttcta
      841 ttttcccttc aggaccagat attagtaaat ctaccattta aacaaaggta atattgaact
      901 aaagcaaatt tgtaaatata gattaaaatt atttatgaat agtcttggtt ttaacaaaca
      961 aaataaagac acaaaggttg tcattgcaat gtcaggagga gtcgatagct ccgttgcagc
     1021 tacaatgctt aaaaaagaag gatatgacat tattggaata acaatgaaac tatatagtca
     1081 aactaaggca aaaagttcta aatcttgttg cgcaggaaga gatattgaag atgctaaaaa
     1141 agtggctgag caacatgatt ttcctcatat aactctagat taccaagata aatttttttc
     1201 aggagtaata gataatttta ttgaaacata tgccgaagga caaacgcccg ttccttgcat
     1261 tcagtgcaat caaactgtta aatttagaga tttattgaac gaagcaaaag ctatgaatgc
     1321 agatactctg gttacagggc attacgtaag gcgactagag gacaaatcaa acgtgaaatt
     1381 atttaaagct aaagatgcat ctaaagacca aagttatttc ctttttgcaa ctcttaaaga
     1441 tcagttagat tatttgcgtt ttccattggg caattacctc aaatcagaag taagaaaaat
     1501 ggcagaaaat tttaaattag aagttagtga taaacctgac agtcaagata tttgctttgt
     1561 tacttctagt tcgtatagag atctcattaa taaattaaaa ccagaattga atattgaagg
     1621 aaatttttta gattctgagg gcaatatttt aggaaagcac aagggaattg ctaattatac
     1681 aatcggtcaa agaagaggat tgggagttgg aggttcaa
//
LOCUS       DTVV01000545            2202 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  MAG TPA_asm: Candidatus Pelagibacterales bacterium isolate
            UWMA-0142 k99_997504, whole genome shotgun sequence.
ACCESSION   DTVV01000545 DTVV01000000
VERSION     DTVV01000545.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967490
            Sequence Read Archive: SRR8626153
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pelagibacterales bacterium (marine metagenome)
  ORGANISM  Candidatus Pelagibacterales bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus
            Pelagibacterales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.15x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 16:01:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,299
            CDSs (total)                      :: 1,290
            Genes (coding)                    :: 1,266
            CDSs (with protein)               :: 1,266
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 17 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Pelagibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_997504"
                     /isolate="UWMA-0142"
                     /isolation_source="Dilute Hydrothermal Plume"
                     /db_xref="taxon:2026776"
                     /environmental_sample
                     /geo_loc_name="Atlantic Ocean: Mid Cayman Rise"
                     /lat_lon="18.37636666 N 81.79808333 W"
                     /collection_date="2012-01-18"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="EYO86_06485"
     CDS             <1..871
                     /locus_tag="EYO86_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Leu/Ile/Val-binding protein"
                     /protein_id="HIC42715.1"
                     /translation="RAKIPWVNGESTSPKLTQRGFKYFFRVTPHDGEFTQLMFEFIND
                     FNSKGGNLKTIGILHEDTLWGTDSGSTQERMAGEQGFDVVEKISYKAKTTSLSSEVQR
                     LKAKNPDVLMPSSYTADAYLFLNTAQELDYNPQILLAQNAGYTDPKFIETMGSKAEGV
                     ITRSPFNTDLATTIPLIGKINELFKIHSGGRDLSDVPARAFTGFMALAQAINNAGSTD
                     SEKIRQALVDLDIDSSSLIVPYHGVKFGPDGQNEKTRGILMQVQNGKYCTVYPFELAA
                     CELKYPMPTWAEK"
     gene            complement(1034..1816)
                     /locus_tag="EYO86_06490"
     CDS             complement(1034..1816)
                     /locus_tag="EYO86_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HIC42716.1"
                     /translation="MWYNIEMEVNLNCDLGEKSEHYSGINDQALIEIINTANIACGYH
                     AGSESVIETTIKIAKENNVSIGAHPGFADKKNFGRKRIELKKNELKKLIRDQLEIISG
                     IADAHQWPITHVKPHGALNNMACENFDVGLAIGESVYEFNKDLIYLVLPLSEMEKAAQ
                     QLNLKYACEIFADRNYQDNGQLISRTDPNAMIESFKTASENILEMLHTSSIKCLSGKK
                     IKCQIDSICIHGDGLRAVSIAKELKKVLIENNIQLVNLDKLN"
     gene            1821..>2202
                     /locus_tag="EYO86_06495"
     CDS             1821..>2202
                     /locus_tag="EYO86_06495"
                     /inference="COORDINATES: protein motif:HMM:PF02682.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HIC42717.1"
                     /translation="MLKHINQISEQAFLLDFGSEINIQINKYVISFADCILQEIEKDN
                     HLKIINCVPSYNKILIQFNAFSDTKTKIIDFLHSLNQKEITHVNKKEIVEIPICYDEE
                     FALDIIDVSKKTRITKSNIIKLHLN"
BASE COUNT          763 a          342 c          329 g          768 t
ORIGIN      
        1 acgtgctaaa ataccttggg tgaatggaga atctacttca ccaaaattaa ctcaaagagg
       61 ttttaaatac ttctttaggg ttactccgca tgatggagag ttcactcaat tgatgtttga
      121 attcataaat gattttaata gtaaaggtgg aaatttaaaa actattggaa ttcttcatga
      181 agatactctt tggggtactg acagtggctc aactcaagaa agaatggctg gagaacaagg
      241 ttttgatgtt gtagagaaaa ttagttataa agcaaaaaca acatctttga gttctgaagt
      301 acaaaggctt aaagctaaaa atccagatgt gttaatgcca tcatcttata cagctgatgc
      361 gtatctcttt ttaaacacag ctcaagagtt ggattacaac ccacaaatac ttcttgctca
      421 aaatgcagga tatacagatc ctaaatttat cgaaacaatg ggtagtaaag ctgaaggagt
      481 tattacaaga tctccattta atactgattt agctactaca atccctttga ttggaaaaat
      541 taatgaactg tttaaaatac attccggagg aagagattta tcagatgtcc cagcaagggc
      601 attcacagga tttatggctc tagcacaagc aatcaacaat gctggatcaa cagattctga
      661 gaaaatcaga caagcacttg ttgatctgga tattgattct agctctttga ttgttcctta
      721 ccatggagtt aaatttggac cagacggtca aaatgaaaaa actcgaggaa tattaatgca
      781 agttcaaaat ggcaaatact gtacagtcta tccatttgaa ttagctgcat gtgaactaaa
      841 gtatcctatg cctacatggg ctgaaaaata atttttactt cattattaaa agggacaata
      901 tgaaaatatt gtccctgaat tatttaataa gttattaaaa tagcaatttt tttttacaat
      961 tttttttaag agtactgctg tactacaaag cattcagtac tccttatggt ttatggaata
     1021 attataaaat aaattaattt aatttgtcta agtttactaa ctgaatattg ttttctatta
     1081 aaactttttt taattctttt gctatagaaa ctgcccttaa gccatctcca tgaatacata
     1141 tagaatcaat ttgacatttt atttttttac cagataagca tttaatggaa gaggtgtgca
     1201 gcatttctaa tatgttttct gaagctgttt tgaaactttc tatcattgca ttaggatcag
     1261 ttcgtgaaat taattggcca ttatcttgat aatttctgtc agcaaaaatc tcgcatgcat
     1321 atttcagatt taactgttga gctgcttttt ccatttcact taaaggcaaa actaaataaa
     1381 ttagatcttt attaaattcg tagacactct ctccaatagc aagtcccaca tcaaaatttt
     1441 cacatgccat gttatttagt gctccgtgag gttttacatg tgttattggc cattggtgag
     1501 catcagcaat accgcttata atttctagtt gatctctaat aagttttttt aattcatttt
     1561 tttttaattc aatgcgttta cgaccaaaat tttttttatc agcgaatcca ggatgagcgc
     1621 caatgcttac gttattttct ttagctattt ttatagtagt ttcaattaca gattcactac
     1681 ctgcatgata tccacaagca atgttcgcag tgttaattat ttcaattaaa gcttggtcgt
     1741 taataccgct ataatgctct gatttttctc caagatcgca gtttaaattc acttccattt
     1801 ctatattata ccacataact atgttgaaac acatcaatca aataagtgaa caagcttttt
     1861 tgttagattt tggttcagag ataaatattc agataaacaa atatgttatt tcttttgctg
     1921 attgtatctt acaagaaata gaaaaagata atcatttaaa aataataaat tgtgtacctt
     1981 cttataataa aattctaatt cagtttaatg ctttctctga cacaaaaaca aaaataatag
     2041 attttttaca ttctttaaat caaaaagaaa ttacccatgt taataaaaaa gaaatcgttg
     2101 aaatacccat atgttatgat gaggaatttg ctttggatat aattgatgtt tctaaaaaaa
     2161 cacgaataac caaaagcaat ataattaaat tacatctaaa ca
//
