Source Name	Characteristics[organism]	Characteristics[taxonomy_id]	Characteristics[collection_date]	Characteristics[geo_loc_name]	Characteristics[strain]	Characteristics[age]	Characteristics[biological_replicate]	Characteristics[sex]	Characteristics[tissue]	Characteristics[treatment]	Comment[BioSample]	Comment[sample_title]	Protocol REF	Sample Name	Protocol REF	Parameter Value[Post extraction]	Parameter Value[Derivatization]	Extract Name	Protocol REF	Parameter Value[Chromatography instrument]	Parameter Value[Autosampler model]	Parameter Value[Column model]	Parameter Value[Column type]	Parameter Value[Guard column]	Labeled Extract Name	Label	Protocol REF	Parameter Value[Scan polarity]	Parameter Value[Scan m/z range]	Parameter Value[Instrument]	Parameter Value[Ion source]	Parameter Value[Mass analyzer]	Assay Name	Comment[technical_replicate]	Raw Data File	Protocol REF	Processed Data File	Protocol REF	Metabolite Assignment File	Comment[maf_value_unit]	Factor Value[diet]
02_01	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Complete holidic medium with total 40%AA for 4 days from day2	SAMD00778043	NEAA_Ctrl_diet_1	Sample collection	02_01	Extraction	100 microL ultra pure water		02_01	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_01		raw/025_25_Ctrl_1_025.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Complete holidic medium
02_02	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Complete holidic medium with total 40%AA for 4 days from day2	SAMD00778044	NEAA_Ctrl_diet_2	Sample collection	02_02	Extraction	100 microL ultra pure water		02_02	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_02		raw/037_37_Ctrl_2_037.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Complete holidic medium
02_03	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Complete holidic medium with total 40%AA for 4 days from day2	SAMD00778045	NEAA_Ctrl_diet_3	Sample collection	02_03	Extraction	100 microL ultra pure water		02_03	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_03		raw/049_49_Ctrl_3_049.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Complete holidic medium
02_04	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Complete holidic medium with total 40%AA for 4 days from day2	SAMD00778046	NEAA_Ctrl_diet_4	Sample collection	02_04	Extraction	100 microL ultra pure water		02_04	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_04		raw/061_61_Ctrl_4_061.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Complete holidic medium
02_05	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Alanine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778047	NEAA_Ala_depletion_1	Sample collection	02_05	Extraction	100 microL ultra pure water		02_05	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_05		raw/026_26_Ala_1_026.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Alanine depleted holidic medium
02_06	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Alanine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778048	NEAA_Ala_depletion_2	Sample collection	02_06	Extraction	100 microL ultra pure water		02_06	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_06		raw/038_38_Ala_2_038.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Alanine depleted holidic medium
02_07	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Alanine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778049	NEAA_Ala_depletion_3	Sample collection	02_07	Extraction	100 microL ultra pure water		02_07	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_07		raw/050_50_Ala_3_050.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Alanine depleted holidic medium
02_08	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Alanine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778050	NEAA_Ala_depletion_4	Sample collection	02_08	Extraction	100 microL ultra pure water		02_08	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_08		raw/062_62_Ala_4_062.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Alanine depleted holidic medium
02_09	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Asparagine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778051	NEAA_Asn_depletion_1	Sample collection	02_09	Extraction	100 microL ultra pure water		02_09	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_09		raw/027_27_Asn_1_027.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Asparagine depleted holidic medium
02_10	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Asparagine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778052	NEAA_Asn_depletion_2	Sample collection	02_10	Extraction	100 microL ultra pure water		02_10	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_10		raw/039_39_Asn_2_039.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Asparagine depleted holidic medium
02_11	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Asparagine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778053	NEAA_Asn_depletion_3	Sample collection	02_11	Extraction	100 microL ultra pure water		02_11	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_11		raw/051_51_Asn_3_051.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Asparagine depleted holidic medium
02_12	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Asparagine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778054	NEAA_Asn_depletion_4	Sample collection	02_12	Extraction	100 microL ultra pure water		02_12	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_12		raw/063_63_Asn_4_063.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Asparagine depleted holidic medium
02_13	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Aspartic acid depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778055	NEAA_Asp_depletion_1	Sample collection	02_13	Extraction	100 microL ultra pure water		02_13	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_13		raw/028_28_Asp_1_028.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Aspartic acid depleted holidic medium
02_14	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Aspartic acid depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778056	NEAA_Asp_depletion_2	Sample collection	02_14	Extraction	100 microL ultra pure water		02_14	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_14		raw/040_40_Asp_2_040.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Aspartic acid depleted holidic medium
02_15	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Aspartic acid depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778057	NEAA_Asp_depletion_3	Sample collection	02_15	Extraction	100 microL ultra pure water		02_15	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_15		raw/052_52_Asp_3_052.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Aspartic acid depleted holidic medium
02_16	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Aspartic acid depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778058	NEAA_Asp_depletion_4	Sample collection	02_16	Extraction	100 microL ultra pure water		02_16	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_16		raw/064_64_Asp_4_064.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Aspartic acid depleted holidic medium
02_17	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Cysteine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778059	NEAA_Cys_depletion_1	Sample collection	02_17	Extraction	100 microL ultra pure water		02_17	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_17		raw/029_29_Cys_1_029.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Cysteine depleted holidic medium
02_18	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Cysteine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778060	NEAA_Cys_depletion_2	Sample collection	02_18	Extraction	100 microL ultra pure water		02_18	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_18		raw/041_41_Cys_2_041.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Cysteine depleted holidic medium
02_19	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Cysteine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778061	NEAA_Cys_depletion_3	Sample collection	02_19	Extraction	100 microL ultra pure water		02_19	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_19		raw/053_53_Cys_3_053.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Cysteine depleted holidic medium
02_20	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Cysteine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778062	NEAA_Cys_depletion_4	Sample collection	02_20	Extraction	100 microL ultra pure water		02_20	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_20		raw/065_65_Cys_4_065.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Cysteine depleted holidic medium
02_21	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Glutamine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778063	NEAA_Gln_depletion_1	Sample collection	02_21	Extraction	100 microL ultra pure water		02_21	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_21		raw/030_30_Gln_1_030.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glutamine depleted holidic medium
02_22	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Glutamine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778064	NEAA_Gln_depletion_2	Sample collection	02_22	Extraction	100 microL ultra pure water		02_22	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_22		raw/042_42_Gln_2_042.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glutamine depleted holidic medium
02_23	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Glutamine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778065	NEAA_Gln_depletion_3	Sample collection	02_23	Extraction	100 microL ultra pure water		02_23	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_23		raw/054_54_Gln_3_054.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glutamine depleted holidic medium
02_24	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Glutamine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778066	NEAA_Gln_depletion_4	Sample collection	02_24	Extraction	100 microL ultra pure water		02_24	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_24		raw/066_66_Gln_4_066.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glutamine depleted holidic medium
02_25	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Glutamic acid depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778067	NEAA_Glu_depletion_1	Sample collection	02_25	Extraction	100 microL ultra pure water		02_25	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_25		raw/031_31_Glu_1_031.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glutamic acid depleted holidic medium
02_26	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Glutamic acid depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778068	NEAA_Glu_depletion_2	Sample collection	02_26	Extraction	100 microL ultra pure water		02_26	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_26		raw/043_43_Glu_2_043.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glutamic acid depleted holidic medium
02_27	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Glutamic acid depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778069	NEAA_Glu_depletion_3	Sample collection	02_27	Extraction	100 microL ultra pure water		02_27	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_27		raw/055_55_Glu_3_055.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glutamic acid depleted holidic medium
02_28	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Glutamic acid depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778070	NEAA_Glu_depletion_4	Sample collection	02_28	Extraction	100 microL ultra pure water		02_28	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_28		raw/067_67_Glu_4_067.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glutamic acid depleted holidic medium
02_29	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Glycine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778071	NEAA_Gly_depletion_1	Sample collection	02_29	Extraction	100 microL ultra pure water		02_29	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_29		raw/032_32_Gly_1_032.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glycine depleted holidic medium
02_30	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Glycine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778072	NEAA_Gly_depletion_2	Sample collection	02_30	Extraction	100 microL ultra pure water		02_30	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_30		raw/044_44_Gly_2_044.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glycine depleted holidic medium
02_31	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Glycine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778073	NEAA_Gly_depletion_3	Sample collection	02_31	Extraction	100 microL ultra pure water		02_31	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_31		raw/056_56_Gly_3_056.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glycine depleted holidic medium
02_32	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Glycine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778074	NEAA_Gly_depletion_4	Sample collection	02_32	Extraction	100 microL ultra pure water		02_32	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_32		raw/068_68_Gly_4_068.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Glycine depleted holidic medium
02_33	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Proline depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778075	NEAA_Pro_depletion_1	Sample collection	02_33	Extraction	100 microL ultra pure water		02_33	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_33		raw/033_33_Pro_1_033.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Proline depleted holidic medium
02_34	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Proline depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778076	NEAA_Pro_depletion_2	Sample collection	02_34	Extraction	100 microL ultra pure water		02_34	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_34		raw/045_45_Pro_2_045.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Proline depleted holidic medium
02_35	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Proline depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778077	NEAA_Pro_depletion_3	Sample collection	02_35	Extraction	100 microL ultra pure water		02_35	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_35		raw/057_57_Pro_3_057.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Proline depleted holidic medium
02_36	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Proline depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778078	NEAA_Pro_depletion_4	Sample collection	02_36	Extraction	100 microL ultra pure water		02_36	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_36		raw/069_69_Pro_4_069.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Proline depleted holidic medium
02_37	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Serine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778079	NEAA_Ser_depletion_1	Sample collection	02_37	Extraction	100 microL ultra pure water		02_37	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_37		raw/034_34_Ser_1_034.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Serine depleted holidic medium
02_38	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Serine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778080	NEAA_Ser_depletion_2	Sample collection	02_38	Extraction	100 microL ultra pure water		02_38	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_38		raw/046_46_Ser_2_046.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Serine depleted holidic medium
02_39	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Serine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778081	NEAA_Ser_depletion_3	Sample collection	02_39	Extraction	100 microL ultra pure water		02_39	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_39		raw/058_58_Ser_3_058.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Serine depleted holidic medium
02_40	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Serine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778082	NEAA_Ser_depletion_4	Sample collection	02_40	Extraction	100 microL ultra pure water		02_40	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_40		raw/070_70_Ser_4_070.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Serine depleted holidic medium
02_41	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate1	female	Whole body	Tyrosine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778083	NEAA_Tyr_depletion_1	Sample collection	02_41	Extraction	100 microL ultra pure water		02_41	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_41		raw/035_35_Tyr_1_035.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Tyrosine depleted holidic medium
02_42	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate2	female	Whole body	Tyrosine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778084	NEAA_Tyr_depletion_2	Sample collection	02_42	Extraction	100 microL ultra pure water		02_42	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_42		raw/047_47_Tyr_2_047.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Tyrosine depleted holidic medium
02_43	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate3	female	Whole body	Tyrosine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778085	NEAA_Tyr_depletion_3	Sample collection	02_43	Extraction	100 microL ultra pure water		02_43	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_43		raw/059_59_Tyr_3_059.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Tyrosine depleted holidic medium
02_44	Drosophila melanogaster	7227	2023-05-27	Japan:Hyogo, Kobe, RIKEN	Canton-S	day6	biological_replicate4	female	Whole body	Tyrosine depleted holidic medium with total 40%AA for 4 days from day 2	SAMD00778086	NEAA_Tyr_depletion_4	Sample collection	02_44	Extraction	100 microL ultra pure water		02_44	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	alternating	use MRM method	LCMS-8060NX (Shimadzu)	heated electrospray ionization	triple quadrupole	02_44		raw/071_71_Tyr_4_071.lcd	Data processing	processed/20230626_NEAA-_MS_summary.xlsx	Metabolite identification	MTBKS239.maf.txt	micro mole/L	Tyrosine depleted holidic medium
