Source Name Characteristics[organism] Characteristics[taxonomy_id] Characteristics[strain] Characteristics[genotype] Characteristics[cell_line] Characteristics[disease] Characteristics[tissue] Characteristics[sample_type] Characteristics[stimulus] Characteristics[treatment] Characteristics[compound] Characteristics[amount] Unit[mole based unit] Comment[BioSample] Comment[sample_title] Protocol REF Comment[Protocol subtype] Sample Name Protocol REF Parameter Value[Post extraction] Parameter Value[Derivatization] Extract Name Protocol REF Parameter Value[Chromatography instrument] Parameter Value[Autosampler model] Parameter Value[Column model] Parameter Value[Column type] Parameter Value[Guard column] Labeled Extract Name Label Protocol REF Parameter Value[Scan polarity] Parameter Value[Scan m/z range] Parameter Value[Instrument] Parameter Value[Ion source] Parameter Value[Mass analyzer] Assay Name Comment[technical_replicate] Raw Data File Protocol REF Processed Data File Protocol REF Metabolite Assignment File Factor Value[genotype] Factor Value[cell_line] Factor Value[sample_type] Factor Value[stimulus] Factor Value[treatment] Factor Value[amount] Unit[mole based unit] HEK293T Homo sapiens 9606 HEK293T SAMD00424292 Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-1 Sample collection Cell sample preparation Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-1 Extraction 100 microL acetonitrile alkylation Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-1 raw/Figure.2a_1_PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T none HEK293T Homo sapiens 9606 HEK293T SAMD00424293 Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-2 Sample collection Cell sample preparation Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-2 Extraction 100 microL acetonitrile alkylation Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-2 raw/Figure.2a_1_PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T none HEK293T Homo sapiens 9606 HEK293T SAMD00424294 Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-3 Sample collection Cell sample preparation Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-3 Extraction 100 microL acetonitrile alkylation Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_1 PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-3 raw/Figure.2a_1_PI_PI3P_PI4P_PI35P2_PI45P2_PIP3-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T none HEK293T Homo sapiens 9606 HEK293T expression IpgD SAMD00424295 Figure.2a_2 PI5P-1 Sample collection Cell sample preparation Figure.2a_2 PI5P-1 Extraction 100 microL acetonitrile alkylation Figure.2a_2 PI5P-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_2 PI5P-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_2 PI5P-1 raw/Figure.2a_2_PI5P-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression IpgD HEK293T Homo sapiens 9606 HEK293T expression IpgD SAMD00424296 Figure.2a_2 PI5P-2 Sample collection Cell sample preparation Figure.2a_2 PI5P-2 Extraction 100 microL acetonitrile alkylation Figure.2a_2 PI5P-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_2 PI5P-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_2 PI5P-2 raw/Figure.2a_2_PI5P-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression IpgD HEK293T Homo sapiens 9606 HEK293T expression IpgD SAMD00424297 Figure.2a_2 PI5P-3 Sample collection Cell sample preparation Figure.2a_2 PI5P-3 Extraction 100 microL acetonitrile alkylation Figure.2a_2 PI5P-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_2 PI5P-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_2 PI5P-3 raw/Figure.2a_2_PI5P-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression IpgD HEK293T Homo sapiens 9606 HEK293T H2O2 SAMD00424298 Figure.2a_3 PI34P2-1 Sample collection Cell sample preparation Figure.2a_3 PI34P2-1 Extraction 100 microL acetonitrile alkylation Figure.2a_3 PI34P2-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_3 PI34P2-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_3 PI34P2-1 raw/Figure.2a_3_PI34P2-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T H2O2 HEK293T Homo sapiens 9606 HEK293T H2O2 SAMD00424299 Figure.2a_3 PI34P2-2 Sample collection Cell sample preparation Figure.2a_3 PI34P2-2 Extraction 100 microL acetonitrile alkylation Figure.2a_3 PI34P2-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_3 PI34P2-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_3 PI34P2-2 raw/Figure.2a_3_PI34P2-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T H2O2 HEK293T Homo sapiens 9606 HEK293T H2O2 SAMD00424300 Figure.2a_3 PI34P2-3 Sample collection Cell sample preparation Figure.2a_3 PI34P2-3 Extraction 100 microL acetonitrile alkylation Figure.2a_3 PI34P2-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2a_3 PI34P2-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2a_3 PI34P2-3 raw/Figure.2a_3_PI34P2-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T H2O2 HeLa Homo sapiens 9606 HeLa Adenocarcinoma DMSO SAMD00424301 Figure.2b_1 PIK-III 0M-1 Sample collection Cell sample preparation Figure.2b_1 PIK-III 0M-1 Extraction 100 microL acetonitrile alkylation Figure.2b_1 PIK-III 0M-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2b_1 PIK-III 0M-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2b_1 PIK-III 0M-1 raw/Figure.2b_1_PIK-III_0M-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HeLa DMSO HeLa Homo sapiens 9606 HeLa Adenocarcinoma DMSO SAMD00424302 Figure.2b_1 PIK-III 0M-2 Sample collection Cell sample preparation Figure.2b_1 PIK-III 0M-2 Extraction 100 microL acetonitrile alkylation Figure.2b_1 PIK-III 0M-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2b_1 PIK-III 0M-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2b_1 PIK-III 0M-2 raw/Figure.2b_1_PIK-III_0M-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HeLa DMSO HeLa Homo sapiens 9606 HeLa Adenocarcinoma DMSO SAMD00424303 Figure.2b_1 PIK-III 0M-3 Sample collection Cell sample preparation Figure.2b_1 PIK-III 0M-3 Extraction 100 microL acetonitrile alkylation Figure.2b_1 PIK-III 0M-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2b_1 PIK-III 0M-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2b_1 PIK-III 0M-3 raw/Figure.2b_1_PIK-III_0M-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HeLa DMSO HeLa Homo sapiens 9606 HeLa Adenocarcinoma DMSO SAMD00424304 Figure.2b_1 PIK-III 0M-4 Sample collection Cell sample preparation Figure.2b_1 PIK-III 0M-4 Extraction 100 microL acetonitrile alkylation Figure.2b_1 PIK-III 0M-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2b_1 PIK-III 0M-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2b_1 PIK-III 0M-4 raw/Figure.2b_1_PIK-III_0M-4.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HeLa DMSO HeLa Homo sapiens 9606 HeLa Adenocarcinoma PIK-III SAMD00424305 Figure.2b_2 PIK-III 5microM-1 Sample collection Cell sample preparation Figure.2b_2 PIK-III 5microM-1 Extraction 100 microL acetonitrile alkylation Figure.2b_2 PIK-III 5microM-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2b_2 PIK-III 5microM-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2b_2 PIK-III 5microM-1 raw/Figure.2b_2_PIK-III_5microM-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HeLa PIK-III HeLa Homo sapiens 9606 HeLa Adenocarcinoma PIK-III SAMD00424306 Figure.2b_2 PIK-III 5microM-2 Sample collection Cell sample preparation Figure.2b_2 PIK-III 5microM-2 Extraction 100 microL acetonitrile alkylation Figure.2b_2 PIK-III 5microM-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2b_2 PIK-III 5microM-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2b_2 PIK-III 5microM-2 raw/Figure.2b_2_PIK-III_5microM-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HeLa PIK-III HeLa Homo sapiens 9606 HeLa Adenocarcinoma PIK-III SAMD00424307 Figure.2b_2 PIK-III 5microM-3 Sample collection Cell sample preparation Figure.2b_2 PIK-III 5microM-3 Extraction 100 microL acetonitrile alkylation Figure.2b_2 PIK-III 5microM-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2b_2 PIK-III 5microM-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2b_2 PIK-III 5microM-3 raw/Figure.2b_2_PIK-III_5microM-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HeLa PIK-III HeLa Homo sapiens 9606 HeLa Adenocarcinoma PIK-III SAMD00424308 Figure.2b_2 PIK-III 5microM-4 Sample collection Cell sample preparation Figure.2b_2 PIK-III 5microM-4 Extraction 100 microL acetonitrile alkylation Figure.2b_2 PIK-III 5microM-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2b_2 PIK-III 5microM-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2b_2 PIK-III 5microM-4 raw/Figure.2b_2_PIK-III_5microM-4.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HeLa PIK-III PC3 Homo sapiens 9606 PC3 Prostate cancer SAMD00424309 Figure.2c_1 None-1 Sample collection Cell sample preparation Figure.2c_1 None-1 Extraction 100 microL acetonitrile alkylation Figure.2c_1 None-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_1 None-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_1 None-1 raw/Figure.2c_1_None-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 none none PC3 Homo sapiens 9606 PC3 Prostate cancer SAMD00424310 Figure.2c_1 None-2 Sample collection Cell sample preparation Figure.2c_1 None-2 Extraction 100 microL acetonitrile alkylation Figure.2c_1 None-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_1 None-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_1 None-2 raw/Figure.2c_1_None-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 none none PC3 Homo sapiens 9606 PC3 Prostate cancer SAMD00424311 Figure.2c_1 None-3 Sample collection Cell sample preparation Figure.2c_1 None-3 Extraction 100 microL acetonitrile alkylation Figure.2c_1 None-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_1 None-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_1 None-3 raw/Figure.2c_1_None-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 none none PC3 Homo sapiens 9606 PC3 Prostate cancer EGF SAMD00424312 Figure.2c_2 EGF-1 Sample collection Cell sample preparation Figure.2c_2 EGF-1 Extraction 100 microL acetonitrile alkylation Figure.2c_2 EGF-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_2 EGF-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_2 EGF-1 raw/Figure.2c_2_EGF-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 EGF none PC3 Homo sapiens 9606 PC3 Prostate cancer EGF SAMD00424313 Figure.2c_2 EGF-2 Sample collection Cell sample preparation Figure.2c_2 EGF-2 Extraction 100 microL acetonitrile alkylation Figure.2c_2 EGF-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_2 EGF-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_2 EGF-2 raw/Figure.2c_2_EGF-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 EGF none PC3 Homo sapiens 9606 PC3 Prostate cancer EGF SAMD00424314 Figure.2c_2 EGF-3 Sample collection Cell sample preparation Figure.2c_2 EGF-3 Extraction 100 microL acetonitrile alkylation Figure.2c_2 EGF-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_2 EGF-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_2 EGF-3 raw/Figure.2c_2_EGF-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 EGF none PC3 Homo sapiens 9606 PC3 Prostate cancer EGF copanlisib SAMD00424315 Figure.2c_3 EGF_plus_copanlisib-1 Sample collection Cell sample preparation Figure.2c_3 EGF_plus_copanlisib-1 Extraction 100 microL acetonitrile alkylation Figure.2c_3 EGF_plus_copanlisib-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_3 EGF_plus_copanlisib-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_3 EGF_plus_copanlisib-1 raw/Figure.2c_3_EGF_plus_copanlisib-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 EGF copanlisib PC3 Homo sapiens 9606 PC3 Prostate cancer EGF copanlisib SAMD00424316 Figure.2c_3 EGF_plus_copanlisib-2 Sample collection Cell sample preparation Figure.2c_3 EGF_plus_copanlisib-2 Extraction 100 microL acetonitrile alkylation Figure.2c_3 EGF_plus_copanlisib-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_3 EGF_plus_copanlisib-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_3 EGF_plus_copanlisib-2 raw/Figure.2c_3_EGF_plus_copanlisib-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 EGF copanlisib PC3 Homo sapiens 9606 PC3 Prostate cancer EGF copanlisib SAMD00424317 Figure.2c_3 EGF_plus_copanlisib-3 Sample collection Cell sample preparation Figure.2c_3 EGF_plus_copanlisib-3 Extraction 100 microL acetonitrile alkylation Figure.2c_3 EGF_plus_copanlisib-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.2c_3 EGF_plus_copanlisib-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.2c_3 EGF_plus_copanlisib-3 raw/Figure.2c_3_EGF_plus_copanlisib-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PC3 EGF copanlisib C57BL/6J mouse prostate Mus musculus 10090 C57BL/6J wild type Prostate SAMD00424328 Figure.3_1 WT-1 Sample collection Tissue sample preparation Figure.3_1 WT-1 Extraction 100 microL acetonitrile alkylation Figure.3_1 WT-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.3_1 WT-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.3_1 WT-1 raw/Figure.3_1_WT-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt wild type C57BL/6J mouse prostate Mus musculus 10090 C57BL/6J wild type Prostate SAMD00424329 Figure.3_1 WT-2 Sample collection Tissue sample preparation Figure.3_1 WT-2 Extraction 100 microL acetonitrile alkylation Figure.3_1 WT-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.3_1 WT-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.3_1 WT-2 raw/Figure.3_1_WT-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt wild type C57BL/6J mouse prostate Mus musculus 10090 C57BL/6J wild type Prostate SAMD00424330 Figure.3_1 WT-3 Sample collection Tissue sample preparation Figure.3_1 WT-3 Extraction 100 microL acetonitrile alkylation Figure.3_1 WT-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.3_1 WT-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.3_1 WT-3 raw/Figure.3_1_WT-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt wild type C57BL/6J mouse prostate Mus musculus 10090 C57BL/6J PbCre4_Pten_flox_flox Prostate SAMD00424331 Figure.3_2 PbCre4_Pten_flox_flox-1 Sample collection Tissue sample preparation Figure.3_2 PbCre4_Pten_flox_flox-1 Extraction 100 microL acetonitrile alkylation Figure.3_2 PbCre4_Pten_flox_flox-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.3_2 PbCre4_Pten_flox_flox-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.3_2 PbCre4_Pten_flox_flox-1 raw/Figure.3_2_PbCre4_Pten_flox_flox-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PbCre4_Pten_flox_flox C57BL/6J mouse prostate Mus musculus 10090 C57BL/6J PbCre4_Pten_flox_flox Prostate SAMD00424332 Figure.3_2 PbCre4_Pten_flox_flox-2 Sample collection Tissue sample preparation Figure.3_2 PbCre4_Pten_flox_flox-2 Extraction 100 microL acetonitrile alkylation Figure.3_2 PbCre4_Pten_flox_flox-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.3_2 PbCre4_Pten_flox_flox-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.3_2 PbCre4_Pten_flox_flox-2 raw/Figure.3_2_PbCre4_Pten_flox_flox-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PbCre4_Pten_flox_flox C57BL/6J mouse prostate Mus musculus 10090 C57BL/6J PbCre4_Pten_flox_flox Prostate SAMD00424291 Figure.3_2 PbCre4_Pten_flox_flox-3 Sample collection Tissue sample preparation Figure.3_2 PbCre4_Pten_flox_flox-3 Extraction 100 microL acetonitrile alkylation Figure.3_2 PbCre4_Pten_flox_flox-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.3_2 PbCre4_Pten_flox_flox-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.3_2 PbCre4_Pten_flox_flox-3 raw/Figure.3_2_PbCre4_Pten_flox_flox-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt PbCre4_Pten_flox_flox conditioned medium Homo sapiens 9606 HEK293T SAMD00424333 Figure.4a_1 Mock-1 Sample collection Preparation of conditioned medium Figure.4a_1 Mock-1 Extraction 100 microL acetonitrile alkylation Figure.4a_1 Mock-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_1 Mock-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_1 Mock-1 raw/Figure.4a_1_Mock-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T none conditioned medium Homo sapiens 9606 HEK293T SAMD00424334 Figure.4a_1 Mock-2 Sample collection Preparation of conditioned medium Figure.4a_1 Mock-2 Extraction 100 microL acetonitrile alkylation Figure.4a_1 Mock-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_1 Mock-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_1 Mock-2 raw/Figure.4a_1_Mock-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T none conditioned medium Homo sapiens 9606 HEK293T SAMD00424335 Figure.4a_1 Mock-3 Sample collection Preparation of conditioned medium Figure.4a_1 Mock-3 Extraction 100 microL acetonitrile alkylation Figure.4a_1 Mock-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_1 Mock-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_1 Mock-3 raw/Figure.4a_1_Mock-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T none conditioned medium Homo sapiens 9606 HEK293T expression PIK3CA E545K SAMD00424336 Figure.4a_2 E545K-1 Sample collection Preparation of conditioned medium Figure.4a_2 E545K-1 Extraction 100 microL acetonitrile alkylation Figure.4a_2 E545K-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_2 E545K-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_2 E545K-1 raw/Figure.4a_2_E545K-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression PIK3CA E545K conditioned medium Homo sapiens 9606 HEK293T expression PIK3CA E545K SAMD00424337 Figure.4a_2 E545K-2 Sample collection Preparation of conditioned medium Figure.4a_2 E545K-2 Extraction 100 microL acetonitrile alkylation Figure.4a_2 E545K-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_2 E545K-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_2 E545K-2 raw/Figure.4a_2_E545K-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression PIK3CA E545K conditioned medium Homo sapiens 9606 HEK293T expression PIK3CA E545K SAMD00424338 Figure.4a_2 E545K-3 Sample collection Preparation of conditioned medium Figure.4a_2 E545K-3 Extraction 100 microL acetonitrile alkylation Figure.4a_2 E545K-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_2 E545K-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_2 E545K-3 raw/Figure.4a_2_E545K-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression PIK3CA E545K conditioned medium Homo sapiens 9606 HEK293T expression PIK3CA H1047R SAMD00424339 Figure.4a_3 H1047R-1 Sample collection Preparation of conditioned medium Figure.4a_3 H1047R-1 Extraction 100 microL acetonitrile alkylation Figure.4a_3 H1047R-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_3 H1047R-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_3 H1047R-1 raw/Figure.4a_3_H1047R-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression PIK3CA H1047R conditioned medium Homo sapiens 9606 HEK293T expression PIK3CA H1047R SAMD00424340 Figure.4a_3 H1047R-2 Sample collection Preparation of conditioned medium Figure.4a_3 H1047R-2 Extraction 100 microL acetonitrile alkylation Figure.4a_3 H1047R-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_3 H1047R-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_3 H1047R-2 raw/Figure.4a_3_H1047R-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression PIK3CA H1047R conditioned medium Homo sapiens 9606 HEK293T expression PIK3CA H1047R SAMD00424341 Figure.4a_3 H1047R-3 Sample collection Preparation of conditioned medium Figure.4a_3 H1047R-3 Extraction 100 microL acetonitrile alkylation Figure.4a_3 H1047R-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4a_3 H1047R-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4a_3 H1047R-3 raw/Figure.4a_3_H1047R-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt HEK293T expression PIK3CA H1047R LNCap Homo sapiens 9606 LNCap Prostate cancer intracellular SAMD00424342 Figure.4b Intracellular-1 Sample collection Cell sample preparation Figure.4b Intracellular-1 Extraction 100 microL acetonitrile alkylation Figure.4b Intracellular-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4b Intracellular-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4b Intracellular-1 raw/Figure.4b_Intracellular-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap intracellular LNCap Homo sapiens 9606 LNCap Prostate cancer intracellular SAMD00424343 Figure.4b Intracellular-2 Sample collection Cell sample preparation Figure.4b Intracellular-2 Extraction 100 microL acetonitrile alkylation Figure.4b Intracellular-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4b Intracellular-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4b Intracellular-2 raw/Figure.4b_Intracellular-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap intracellular LNCap Homo sapiens 9606 LNCap Prostate cancer intracellular SAMD00424344 Figure.4b Intracellular-3 Sample collection Cell sample preparation Figure.4b Intracellular-3 Extraction 100 microL acetonitrile alkylation Figure.4b Intracellular-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4b Intracellular-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4b Intracellular-3 raw/Figure.4b_Intracellular-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap intracellular conditioned medium Homo sapiens 9606 LNCap Prostate cancer extracellular SAMD00424345 Figure.4c Extracellular-1 Sample collection Preparation of conditioned medium Figure.4c Extracellular-1 Extraction 100 microL acetonitrile alkylation Figure.4c Extracellular-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4c Extracellular-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4c Extracellular-1 raw/Figure.4c_Extracellular-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap extracellular conditioned medium Homo sapiens 9606 LNCap Prostate cancer extracellular SAMD00424346 Figure.4c Extracellular-2 Sample collection Preparation of conditioned medium Figure.4c Extracellular-2 Extraction 100 microL acetonitrile alkylation Figure.4c Extracellular-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4c Extracellular-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4c Extracellular-2 raw/Figure.4c_Extracellular-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap extracellular conditioned medium Homo sapiens 9606 LNCap Prostate cancer extracellular SAMD00424347 Figure.4c Extracellular-3 Sample collection Preparation of conditioned medium Figure.4c Extracellular-3 Extraction 100 microL acetonitrile alkylation Figure.4c Extracellular-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4c Extracellular-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4c Extracellular-3 raw/Figure.4c_Extracellular-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap extracellular exosome Homo sapiens 9606 LNCap Prostate cancer exosome SAMD00424348 Figure.4d Exosome-1 Sample collection Preparation of exosomes Figure.4d Exosome-1 Extraction 100 microL acetonitrile alkylation Figure.4d Exosome-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4d Exosome-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4d Exosome-1 raw/Figure.4d_Exosome-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap exosome exosome Homo sapiens 9606 LNCap Prostate cancer exosome SAMD00424349 Figure.4d Exosome-2 Sample collection Preparation of exosomes Figure.4d Exosome-2 Extraction 100 microL acetonitrile alkylation Figure.4d Exosome-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4d Exosome-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4d Exosome-2 raw/Figure.4d_Exosome-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap exosome exosome Homo sapiens 9606 LNCap Prostate cancer exosome SAMD00424350 Figure.4d Exosome-3 Sample collection Preparation of exosomes Figure.4d Exosome-3 Extraction 100 microL acetonitrile alkylation Figure.4d Exosome-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4d Exosome-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4d Exosome-3 raw/Figure.4d_Exosome-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap exosome C57BL/6J mouse plasma Mus musculus 10090 C57BL/6J plasma SAMD00424351 Figure.4e_1 PBS-1 Sample collection Preparation of mouse plasma Figure.4e_1 PBS-1 Extraction 100 microL acetonitrile alkylation Figure.4e_1 PBS-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4e_1 PBS-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4e_1 PBS-1 raw/Figure.4e_1_PBS-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt none C57BL/6J mouse plasma Mus musculus 10090 C57BL/6J plasma SAMD00424352 Figure.4e_1 PBS-2 Sample collection Preparation of mouse plasma Figure.4e_1 PBS-2 Extraction 100 microL acetonitrile alkylation Figure.4e_1 PBS-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4e_1 PBS-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4e_1 PBS-2 raw/Figure.4e_1_PBS-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt none C57BL/6J mouse plasma Mus musculus 10090 C57BL/6J plasma SAMD00424353 Figure.4e_1 PBS-3 Sample collection Preparation of mouse plasma Figure.4e_1 PBS-3 Extraction 100 microL acetonitrile alkylation Figure.4e_1 PBS-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4e_1 PBS-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4e_1 PBS-3 raw/Figure.4e_1_PBS-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt none C57BL/6J mouse plasma Mus musculus 10090 C57BL/6J plasma SAMD00424354 Figure.4e_1 PBS-4 Sample collection Preparation of mouse plasma Figure.4e_1 PBS-4 Extraction 100 microL acetonitrile alkylation Figure.4e_1 PBS-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4e_1 PBS-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4e_1 PBS-4 raw/Figure.4e_1_PBS-4.mzML Data processing Metabolite identification MTBKS201.1.maf.txt none C57BL/6J mouse plasma Mus musculus 10090 C57BL/6J plasma LPS SAMD00424355 Figure.4e_2 LPS-1 Sample collection Preparation of mouse plasma Figure.4e_2 LPS-1 Extraction 100 microL acetonitrile alkylation Figure.4e_2 LPS-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4e_2 LPS-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4e_2 LPS-1 raw/Figure.4e_2_LPS-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LPS C57BL/6J mouse plasma Mus musculus 10090 C57BL/6J plasma LPS SAMD00424356 Figure.4e_2 LPS-2 Sample collection Preparation of mouse plasma Figure.4e_2 LPS-2 Extraction 100 microL acetonitrile alkylation Figure.4e_2 LPS-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4e_2 LPS-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4e_2 LPS-2 raw/Figure.4e_2_LPS-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LPS C57BL/6J mouse plasma Mus musculus 10090 C57BL/6J plasma LPS SAMD00424357 Figure.4e_2 LPS-3 Sample collection Preparation of mouse plasma Figure.4e_2 LPS-3 Extraction 100 microL acetonitrile alkylation Figure.4e_2 LPS-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4e_2 LPS-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4e_2 LPS-3 raw/Figure.4e_2_LPS-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LPS C57BL/6J mouse plasma Mus musculus 10090 C57BL/6J plasma LPS SAMD00424358 Figure.4e_2 LPS-4 Sample collection Preparation of mouse plasma Figure.4e_2 LPS-4 Extraction 100 microL acetonitrile alkylation Figure.4e_2 LPS-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.4e_2 LPS-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.4e_2 LPS-4 raw/Figure.4e_2_LPS-4.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LPS exosome Homo sapiens 9606 LNCap Prostate cancer exosome SAMD00424359 Figure.S5 Exosome-1 Sample collection Preparation of exosomes Figure.S5 Exosome-1 Extraction 100 microL methanol/water 75/25 (v/v) alkylation Figure.S5 Exosome-1 Chromatography UltiMate 3000 (Thermo Fisher Scientific) HTC PAL (CTC Analytics) autosampler InertSustain Bio C18 column (2.1 mm x 150 mm, 1.9 microm; GL Science) reverse phase Figure.S5 Exosome-1 Mass spectrometry positive 5-2000 Thermo Fisher TSQ Vantage electrospray ionization triple quadruple Figure.S5 Exosome-1 raw/Figure.S5_Exosome-1.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap exosome exosome Homo sapiens 9606 LNCap Prostate cancer exosome SAMD00424360 Figure.S5 Exosome-2 Sample collection Preparation of exosomes Figure.S5 Exosome-2 Extraction 100 microL methanol/water 75/25 (v/v) alkylation Figure.S5 Exosome-2 Chromatography UltiMate 3000 (Thermo Fisher Scientific) HTC PAL (CTC Analytics) autosampler InertSustain Bio C18 column (2.1 mm x 150 mm, 1.9 microm; GL Science) reverse phase Figure.S5 Exosome-2 Mass spectrometry positive 5-2000 Thermo Fisher TSQ Vantage electrospray ionization triple quadruple Figure.S5 Exosome-2 raw/Figure.S5_Exosome-2.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap exosome exosome Homo sapiens 9606 LNCap Prostate cancer exosome SAMD00424361 Figure.S5 Exosome-3 Sample collection Preparation of exosomes Figure.S5 Exosome-3 Extraction 100 microL methanol/water 75/25 (v/v) alkylation Figure.S5 Exosome-3 Chromatography UltiMate 3000 (Thermo Fisher Scientific) HTC PAL (CTC Analytics) autosampler InertSustain Bio C18 column (2.1 mm x 150 mm, 1.9 microm; GL Science) reverse phase Figure.S5 Exosome-3 Mass spectrometry positive 5-2000 Thermo Fisher TSQ Vantage electrospray ionization triple quadruple Figure.S5 Exosome-3 raw/Figure.S5_Exosome-3.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap exosome exosome Homo sapiens 9606 LNCap Prostate cancer exosome SAMD00424362 Figure.S5 Exosome-4 Sample collection Preparation of exosomes Figure.S5 Exosome-4 Extraction 100 microL methanol/water 75/25 (v/v) alkylation Figure.S5 Exosome-4 Chromatography UltiMate 3000 (Thermo Fisher Scientific) HTC PAL (CTC Analytics) autosampler InertSustain Bio C18 column (2.1 mm x 150 mm, 1.9 microm; GL Science) reverse phase Figure.S5 Exosome-4 Mass spectrometry positive 5-2000 Thermo Fisher TSQ Vantage electrospray ionization triple quadruple Figure.S5 Exosome-4 raw/Figure.S5_Exosome-4.mzML Data processing Metabolite identification MTBKS201.1.maf.txt LNCap exosome chemical standard unidentified 32644 phosphoinositide 2 femtomole SAMD00424363 Figure.1_1 calibration_curve_2fmol-1 Sample collection Chemical standard Figure.1_1 calibration_curve_2fmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_1 calibration_curve_2fmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_1 calibration_curve_2fmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_1 calibration_curve_2fmol-1 raw/Figure.1_1_calibration_curve_2fmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 2 femtomole chemical standard unidentified 32644 phosphoinositide 2 femtomole SAMD00424364 Figure.1_1 calibration_curve_2fmol-2 Sample collection Chemical standard Figure.1_1 calibration_curve_2fmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_1 calibration_curve_2fmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_1 calibration_curve_2fmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_1 calibration_curve_2fmol-2 raw/Figure.1_1_calibration_curve_2fmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 2 femtomole chemical standard unidentified 32644 phosphoinositide 2 femtomole SAMD00424365 Figure.1_1 calibration_curve_2fmol-3 Sample collection Chemical standard Figure.1_1 calibration_curve_2fmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_1 calibration_curve_2fmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_1 calibration_curve_2fmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_1 calibration_curve_2fmol-3 raw/Figure.1_1_calibration_curve_2fmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 2 femtomole chemical standard unidentified 32644 phosphoinositide 2 femtomole SAMD00424366 Figure.1_1 calibration_curve_2fmol-4 Sample collection Chemical standard Figure.1_1 calibration_curve_2fmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_1 calibration_curve_2fmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_1 calibration_curve_2fmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_1 calibration_curve_2fmol-4 raw/Figure.1_1_calibration_curve_2fmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 2 femtomole chemical standard unidentified 32644 phosphoinositide 5 femtomole SAMD00424367 Figure.1_2 calibration_curve_5fmol-1 Sample collection Chemical standard Figure.1_2 calibration_curve_5fmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_2 calibration_curve_5fmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_2 calibration_curve_5fmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_2 calibration_curve_5fmol-1 raw/Figure.1_2_calibration_curve_5fmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 5 femtomole chemical standard unidentified 32644 phosphoinositide 5 femtomole SAMD00424368 Figure.1_2 calibration_curve_5fmol-2 Sample collection Chemical standard Figure.1_2 calibration_curve_5fmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_2 calibration_curve_5fmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_2 calibration_curve_5fmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_2 calibration_curve_5fmol-2 raw/Figure.1_2_calibration_curve_5fmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 5 femtomole chemical standard unidentified 32644 phosphoinositide 5 femtomole SAMD00424369 Figure.1_2 calibration_curve_5fmol-3 Sample collection Chemical standard Figure.1_2 calibration_curve_5fmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_2 calibration_curve_5fmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_2 calibration_curve_5fmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_2 calibration_curve_5fmol-3 raw/Figure.1_2_calibration_curve_5fmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 5 femtomole chemical standard unidentified 32644 phosphoinositide 5 femtomole SAMD00424370 Figure.1_2 calibration_curve_5fmol-4 Sample collection Chemical standard Figure.1_2 calibration_curve_5fmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_2 calibration_curve_5fmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_2 calibration_curve_5fmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_2 calibration_curve_5fmol-4 raw/Figure.1_2_calibration_curve_5fmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 5 femtomole chemical standard unidentified 32644 phosphoinositide 10 femtomole SAMD00424371 Figure.1_3 calibration_curve_10fmol-1 Sample collection Chemical standard Figure.1_3 calibration_curve_10fmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_3 calibration_curve_10fmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_3 calibration_curve_10fmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_3 calibration_curve_10fmol-1 raw/Figure.1_3_calibration_curve_10fmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 10 femtomole chemical standard unidentified 32644 phosphoinositide 10 femtomole SAMD00424372 Figure.1_3 calibration_curve_10fmol-2 Sample collection Chemical standard Figure.1_3 calibration_curve_10fmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_3 calibration_curve_10fmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_3 calibration_curve_10fmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_3 calibration_curve_10fmol-2 raw/Figure.1_3_calibration_curve_10fmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 10 femtomole chemical standard unidentified 32644 phosphoinositide 10 femtomole SAMD00424373 Figure.1_3 calibration_curve_10fmol-3 Sample collection Chemical standard Figure.1_3 calibration_curve_10fmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_3 calibration_curve_10fmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_3 calibration_curve_10fmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_3 calibration_curve_10fmol-3 raw/Figure.1_3_calibration_curve_10fmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 10 femtomole chemical standard unidentified 32644 phosphoinositide 10 femtomole SAMD00424374 Figure.1_3 calibration_curve_10fmol-4 Sample collection Chemical standard Figure.1_3 calibration_curve_10fmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_3 calibration_curve_10fmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_3 calibration_curve_10fmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_3 calibration_curve_10fmol-4 raw/Figure.1_3_calibration_curve_10fmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 10 femtomole chemical standard unidentified 32644 phosphoinositide 20 femtomole SAMD00424375 Figure.1_4 calibration_curve_20fmol-1 Sample collection Chemical standard Figure.1_4 calibration_curve_20fmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_4 calibration_curve_20fmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_4 calibration_curve_20fmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_4 calibration_curve_20fmol-1 raw/Figure.1_4_calibration_curve_20fmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 20 femtomole chemical standard unidentified 32644 phosphoinositide 20 femtomole SAMD00424376 Figure.1_4 calibration_curve_20fmol-2 Sample collection Chemical standard Figure.1_4 calibration_curve_20fmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_4 calibration_curve_20fmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_4 calibration_curve_20fmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_4 calibration_curve_20fmol-2 raw/Figure.1_4_calibration_curve_20fmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 20 femtomole chemical standard unidentified 32644 phosphoinositide 20 femtomole SAMD00424377 Figure.1_4 calibration_curve_20fmol-3 Sample collection Chemical standard Figure.1_4 calibration_curve_20fmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_4 calibration_curve_20fmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_4 calibration_curve_20fmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_4 calibration_curve_20fmol-3 raw/Figure.1_4_calibration_curve_20fmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 20 femtomole chemical standard unidentified 32644 phosphoinositide 20 femtomole SAMD00424378 Figure.1_4 calibration_curve_20fmol-4 Sample collection Chemical standard Figure.1_4 calibration_curve_20fmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_4 calibration_curve_20fmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_4 calibration_curve_20fmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_4 calibration_curve_20fmol-4 raw/Figure.1_4_calibration_curve_20fmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 20 femtomole chemical standard unidentified 32644 phosphoinositide 50 femtomole SAMD00424379 Figure.1_5 calibration_curve_50fmol-1 Sample collection Chemical standard Figure.1_5 calibration_curve_50fmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_5 calibration_curve_50fmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_5 calibration_curve_50fmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_5 calibration_curve_50fmol-1 raw/Figure.1_5_calibration_curve_50fmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 50 femtomole chemical standard unidentified 32644 phosphoinositide 50 femtomole SAMD00424380 Figure.1_5 calibration_curve_50fmol-2 Sample collection Chemical standard Figure.1_5 calibration_curve_50fmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_5 calibration_curve_50fmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_5 calibration_curve_50fmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_5 calibration_curve_50fmol-2 raw/Figure.1_5_calibration_curve_50fmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 50 femtomole chemical standard unidentified 32644 phosphoinositide 50 femtomole SAMD00424381 Figure.1_5 calibration_curve_50fmol-3 Sample collection Chemical standard Figure.1_5 calibration_curve_50fmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_5 calibration_curve_50fmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_5 calibration_curve_50fmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_5 calibration_curve_50fmol-3 raw/Figure.1_5_calibration_curve_50fmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 50 femtomole chemical standard unidentified 32644 phosphoinositide 50 femtomole SAMD00424382 Figure.1_5 calibration_curve_50fmol-4 Sample collection Chemical standard Figure.1_5 calibration_curve_50fmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_5 calibration_curve_50fmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_5 calibration_curve_50fmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_5 calibration_curve_50fmol-4 raw/Figure.1_5_calibration_curve_50fmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 50 femtomole chemical standard unidentified 32644 phosphoinositide 100 femtomole SAMD00424383 Figure.1_6 calibration_curve_100fmol-1 Sample collection Chemical standard Figure.1_6 calibration_curve_100fmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_6 calibration_curve_100fmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_6 calibration_curve_100fmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_6 calibration_curve_100fmol-1 raw/Figure.1_6_calibration_curve_100fmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 100 femtomole chemical standard unidentified 32644 phosphoinositide 100 femtomole SAMD00424384 Figure.1_6 calibration_curve_100fmol-2 Sample collection Chemical standard Figure.1_6 calibration_curve_100fmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_6 calibration_curve_100fmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_6 calibration_curve_100fmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_6 calibration_curve_100fmol-2 raw/Figure.1_6_calibration_curve_100fmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 100 femtomole chemical standard unidentified 32644 phosphoinositide 100 femtomole SAMD00424385 Figure.1_6 calibration_curve_100fmol-3 Sample collection Chemical standard Figure.1_6 calibration_curve_100fmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_6 calibration_curve_100fmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_6 calibration_curve_100fmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_6 calibration_curve_100fmol-3 raw/Figure.1_6_calibration_curve_100fmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 100 femtomole chemical standard unidentified 32644 phosphoinositide 100 femtomole SAMD00424386 Figure.1_6 calibration_curve_100fmol-4 Sample collection Chemical standard Figure.1_6 calibration_curve_100fmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_6 calibration_curve_100fmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_6 calibration_curve_100fmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_6 calibration_curve_100fmol-4 raw/Figure.1_6_calibration_curve_100fmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 100 femtomole chemical standard unidentified 32644 phosphoinositide 200 femtomole SAMD00424387 Figure.1_7 calibration_curve_200fmol-1 Sample collection Chemical standard Figure.1_7 calibration_curve_200fmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_7 calibration_curve_200fmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_7 calibration_curve_200fmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_7 calibration_curve_200fmol-1 raw/Figure.1_7_calibration_curve_200fmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 200 femtomole chemical standard unidentified 32644 phosphoinositide 200 femtomole SAMD00424388 Figure.1_7 calibration_curve_200fmol-2 Sample collection Chemical standard Figure.1_7 calibration_curve_200fmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_7 calibration_curve_200fmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_7 calibration_curve_200fmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_7 calibration_curve_200fmol-2 raw/Figure.1_7_calibration_curve_200fmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 200 femtomole chemical standard unidentified 32644 phosphoinositide 200 femtomole SAMD00424389 Figure.1_7 calibration_curve_200fmol-3 Sample collection Chemical standard Figure.1_7 calibration_curve_200fmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_7 calibration_curve_200fmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_7 calibration_curve_200fmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_7 calibration_curve_200fmol-3 raw/Figure.1_7_calibration_curve_200fmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 200 femtomole chemical standard unidentified 32644 phosphoinositide 200 femtomole SAMD00424390 Figure.1_7 calibration_curve_200fmol-4 Sample collection Chemical standard Figure.1_7 calibration_curve_200fmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_7 calibration_curve_200fmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_7 calibration_curve_200fmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_7 calibration_curve_200fmol-4 raw/Figure.1_7_calibration_curve_200fmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 200 femtomole chemical standard unidentified 32644 phosphoinositide 500 femtomole SAMD00424391 Figure.1_8 calibration_curve_500fmol-1 Sample collection Chemical standard Figure.1_8 calibration_curve_500fmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_8 calibration_curve_500fmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_8 calibration_curve_500fmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_8 calibration_curve_500fmol-1 raw/Figure.1_8_calibration_curve_500fmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 500 femtomole chemical standard unidentified 32644 phosphoinositide 500 femtomole SAMD00424392 Figure.1_8 calibration_curve_500fmol-2 Sample collection Chemical standard Figure.1_8 calibration_curve_500fmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_8 calibration_curve_500fmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_8 calibration_curve_500fmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_8 calibration_curve_500fmol-2 raw/Figure.1_8_calibration_curve_500fmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 500 femtomole chemical standard unidentified 32644 phosphoinositide 500 femtomole SAMD00424393 Figure.1_8 calibration_curve_500fmol-3 Sample collection Chemical standard Figure.1_8 calibration_curve_500fmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_8 calibration_curve_500fmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_8 calibration_curve_500fmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_8 calibration_curve_500fmol-3 raw/Figure.1_8_calibration_curve_500fmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 500 femtomole chemical standard unidentified 32644 phosphoinositide 500 femtomole SAMD00424394 Figure.1_8 calibration_curve_500fmol-4 Sample collection Chemical standard Figure.1_8 calibration_curve_500fmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_8 calibration_curve_500fmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_8 calibration_curve_500fmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_8 calibration_curve_500fmol-4 raw/Figure.1_8_calibration_curve_500fmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 500 femtomole chemical standard unidentified 32644 phosphoinositide 1 picomole SAMD00424395 Figure.1_9 calibration_curve_1pmol-1 Sample collection Chemical standard Figure.1_9 calibration_curve_1pmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_9 calibration_curve_1pmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_9 calibration_curve_1pmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_9 calibration_curve_1pmol-1 raw/Figure.1_9_calibration_curve_1pmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 1 picomole chemical standard unidentified 32644 phosphoinositide 1 picomole SAMD00424396 Figure.1_9 calibration_curve_1pmol-2 Sample collection Chemical standard Figure.1_9 calibration_curve_1pmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_9 calibration_curve_1pmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_9 calibration_curve_1pmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_9 calibration_curve_1pmol-2 raw/Figure.1_9_calibration_curve_1pmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 1 picomole chemical standard unidentified 32644 phosphoinositide 1 picomole SAMD00424397 Figure.1_9 calibration_curve_1pmol-3 Sample collection Chemical standard Figure.1_9 calibration_curve_1pmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_9 calibration_curve_1pmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_9 calibration_curve_1pmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_9 calibration_curve_1pmol-3 raw/Figure.1_9_calibration_curve_1pmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 1 picomole chemical standard unidentified 32644 phosphoinositide 1 picomole SAMD00424398 Figure.1_9 calibration_curve_1pmol-4 Sample collection Chemical standard Figure.1_9 calibration_curve_1pmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_9 calibration_curve_1pmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_9 calibration_curve_1pmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_9 calibration_curve_1pmol-4 raw/Figure.1_9_calibration_curve_1pmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 1 picomole chemical standard unidentified 32644 phosphoinositide 5 picomole SAMD00424399 Figure.1_10 calibration_curve_5pmol-1 Sample collection Chemical standard Figure.1_10 calibration_curve_5pmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_10 calibration_curve_5pmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_10 calibration_curve_5pmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_10 calibration_curve_5pmol-1 raw/Figure.1_10_calibration_curve_5pmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 5 picomole chemical standard unidentified 32644 phosphoinositide 5 picomole SAMD00424400 Figure.1_10 calibration_curve_5pmol-2 Sample collection Chemical standard Figure.1_10 calibration_curve_5pmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_10 calibration_curve_5pmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_10 calibration_curve_5pmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_10 calibration_curve_5pmol-2 raw/Figure.1_10_calibration_curve_5pmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 5 picomole chemical standard unidentified 32644 phosphoinositide 5 picomole SAMD00424318 Figure.1_10 calibration_curve_5pmol-3 Sample collection Chemical standard Figure.1_10 calibration_curve_5pmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_10 calibration_curve_5pmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_10 calibration_curve_5pmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_10 calibration_curve_5pmol-3 raw/Figure.1_10_calibration_curve_5pmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 5 picomole chemical standard unidentified 32644 phosphoinositide 5 picomole SAMD00424319 Figure.1_10 calibration_curve_5pmol-4 Sample collection Chemical standard Figure.1_10 calibration_curve_5pmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_10 calibration_curve_5pmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_10 calibration_curve_5pmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_10 calibration_curve_5pmol-4 raw/Figure.1_10_calibration_curve_5pmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 5 picomole chemical standard unidentified 32644 phosphoinositide 10 picomole SAMD00424320 Figure.1_11 calibration_curve_10pmol-1 Sample collection Chemical standard Figure.1_11 calibration_curve_10pmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_11 calibration_curve_10pmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_11 calibration_curve_10pmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_11 calibration_curve_10pmol-1 raw/Figure.1_11_calibration_curve_10pmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 10 picomole chemical standard unidentified 32644 phosphoinositide 10 picomole SAMD00424321 Figure.1_11 calibration_curve_10pmol-2 Sample collection Chemical standard Figure.1_11 calibration_curve_10pmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_11 calibration_curve_10pmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_11 calibration_curve_10pmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_11 calibration_curve_10pmol-2 raw/Figure.1_11_calibration_curve_10pmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 10 picomole chemical standard unidentified 32644 phosphoinositide 10 picomole SAMD00424322 Figure.1_11 calibration_curve_10pmol-3 Sample collection Chemical standard Figure.1_11 calibration_curve_10pmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_11 calibration_curve_10pmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_11 calibration_curve_10pmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_11 calibration_curve_10pmol-3 raw/Figure.1_11_calibration_curve_10pmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 10 picomole chemical standard unidentified 32644 phosphoinositide 10 picomole SAMD00424323 Figure.1_11 calibration_curve_10pmol-4 Sample collection Chemical standard Figure.1_11 calibration_curve_10pmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_11 calibration_curve_10pmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_11 calibration_curve_10pmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_11 calibration_curve_10pmol-4 raw/Figure.1_11_calibration_curve_10pmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 10 picomole chemical standard unidentified 32644 phosphoinositide 50 picomole SAMD00424324 Figure.1_12 calibration_curve_50pmol-1 Sample collection Chemical standard Figure.1_12 calibration_curve_50pmol-1 Extraction 100 microL acetonitrile alkylation Figure.1_12 calibration_curve_50pmol-1 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_12 calibration_curve_50pmol-1 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_12 calibration_curve_50pmol-1 raw/Figure.1_12_calibration_curve_50pmol-1.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 50 picomole chemical standard unidentified 32644 phosphoinositide 50 picomole SAMD00424325 Figure.1_12 calibration_curve_50pmol-2 Sample collection Chemical standard Figure.1_12 calibration_curve_50pmol-2 Extraction 100 microL acetonitrile alkylation Figure.1_12 calibration_curve_50pmol-2 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_12 calibration_curve_50pmol-2 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_12 calibration_curve_50pmol-2 raw/Figure.1_12_calibration_curve_50pmol-2.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 50 picomole chemical standard unidentified 32644 phosphoinositide 50 picomole SAMD00424326 Figure.1_12 calibration_curve_50pmol-3 Sample collection Chemical standard Figure.1_12 calibration_curve_50pmol-3 Extraction 100 microL acetonitrile alkylation Figure.1_12 calibration_curve_50pmol-3 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_12 calibration_curve_50pmol-3 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_12 calibration_curve_50pmol-3 raw/Figure.1_12_calibration_curve_50pmol-3.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 50 picomole chemical standard unidentified 32644 phosphoinositide 50 picomole SAMD00424327 Figure.1_12 calibration_curve_50pmol-4 Sample collection Chemical standard Figure.1_12 calibration_curve_50pmol-4 Extraction 100 microL acetonitrile alkylation Figure.1_12 calibration_curve_50pmol-4 Chromatography Nexera X2 HPLC system (Shimadzu) PAL HTC-xt (CTC Analytics) autosampler CHIRALPAK IC-3 column [cellulose tris(3,5-dichlorophenylcarbamate), 2.1 mm x 250 mm, 3 microm; DAICEL] reverse phase Figure.1_12 calibration_curve_50pmol-4 Mass spectrometry positive 5-2000 ABSciex QTRAP6500 electrospray ionization triple quadruple Figure.1_12 calibration_curve_50pmol-4 raw/Figure.1_12_calibration_curve_50pmol-4.mzML Data processing Metabolite identification MTBKS201.2.maf.txt 50 picomole