MAGE-TAB Version	1.1
Comment[MetaboBank accession]	MTBKS210
Study Title	Detection of ALC in royal food
Study Description	To confirm whether a functional compound with small molecular weight (for example acetyl-L-carnitine) is contained in king food and queen food.

Experimental Design	organism part comparison design
Experimental Factor Name	tissue
Experimental Factor Type	tissue

Person Last Name	Mitaka	Sakamoto
Person First Name	Yuki	Takumi
Person Mid Initials
Person Affiliation	Kyoto University	Hamamatsu University School of Medicine
Person Roles	submitter	submitter

PubMed ID
Publication DOI	10.1093/pnasnexus/pgad222

Protocol Name	Sample collection	Extraction	Flow injection analysis	Mass spectrometry	Data processing	Metabolite identification
Protocol Type	Sample collection	Extraction	Flow injection analysis	Mass spectrometry	Data processing	Metabolite identification
Protocol Description	We opened the window of the glass cell at the moment the worker started feeding the king or queen, pinched the feeding worker between the head and thorax with forceps, and immediately collected the food directly from between the feeding worker's mandibles, labrum, and labium using a picker.	The collected royal food sample was reconstituted in 80 µL of isopropanol: acetonitrile: ultrapure water (1:1:1, v/v/v). Then the whole sample was sonicated at 4 °C for 20 min on ice. The sonicated sample was then centrifuged at 94 x g for 5 min at 4 °C. The supernatant was filtered through 0.2 μm Millex syringe driven filter unit	Ultimate 3000 HPLC system (Thermo Fisher Scientific) was used.  5 µL of sample was injected with a flow rate of 200 µL/min for a total run time of 1 minute.	Sample was directly analyzed using flow injection in the quadrupole Orbitrap mass spectrometer coupled to an electrospray ionization (ESI) source (Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer, Thermo Fisher Scientific Inc., USA). MS spectra in the mass range of m/z 100-1,000 were obtained in positive ion mode. Higher energy collisional dissociation (HCD) 15.0 eV was used in positive ion mode for MS/MS fragmentation of ALC and carnitine.	Data not submitted.	Data not submitted.
Protocol Parameters		Post extraction;Derivatization	FIA instrument	Scan polarity;Scan m/z range;Instrument;Ion source;Mass analyzer
Protocol Hardware
Protocol Software

Public Release Date	2023-07-06

Term Source Name
Term Source File
Term Source Version

SDRF File	MTBKS210.sdrf.txt

Comment[Study type]	metabolite target analysis
Comment[Experiment type]	flow injection analysis-mass spectrometry
Comment[Submission type]	FIA-MS
Comment[BioProject]	PRJDB14286
Comment[Related study]
Comment[Contributor]	Takumi Sakamoto, A. S. M. Waliullah
Comment[Submission Date]	2022-10-01
Comment[Last Update Date]	2023-07-06