Source Name Characteristics[sample_name] Characteristics[organism] Characteristics[taxonomy_id] Characteristics[collection_date] Characteristics[isolate] Characteristics[sex] Characteristics[tissue] Characteristics[treatment] Comment[BioSample] Comment[sample_title] Protocol REF Sample Name Protocol REF Parameter Value[Sample mounting] Parameter Value[Sample preservation] Parameter Value[Tissue modification] Parameter Value[Sectioning instrument] Parameter Value[Section thickness] Unit[length] Parameter Value[Matrix] Parameter Value[Matrix application] Protocol REF Parameter Value[Scan polarity] Parameter Value[Scan m/z range] Parameter Value[Instrument] Parameter Value[Instrument manufacturer] Parameter Value[Ion source] Parameter Value[Mass analyzer] Parameter Value[Solvent] Parameter Value[Target material] Parameter Value[Spatial resolution] Unit[length] Parameter Value[Pixel size x] Unit[length] Parameter Value[Pixel size y] Unit[length] Parameter Value[Max count of pixel x] Parameter Value[Max count of pixel y] Parameter Value[Max dimension x] Unit[length] Parameter Value[Max dimension y] Unit[length] Parameter Value[Inlet type] Parameter Value[Detector] Parameter Value[Detector mode] Parameter Value[Resolving power] Parameter Value[Resolving power m/z] Parameter Value[Native spectrum identifier format] Parameter Value[Data file content] Parameter Value[Spectrum representation] Parameter Value[Raw data file format] Parameter Value[Instrument software] Parameter Value[Instrument software version] Parameter Value[Line scan direction] Parameter Value[Line scan sequence] Parameter Value[Scan pattern] Parameter Value[Scan type] Parameter Value[Number of scans] Assay Name Comment[technical_replicate] Raw Data File Protocol REF Parameter Value[Stain] Image Data File Comment[Image Data File md5] Protocol REF Parameter Value[Data processing software] Parameter Value[Data processing software version] Processed Data File Protocol REF Metabolite Assignment File Comment[maf_value_unit] Factor Value[tissue] Factor Value[treatment] Worker whole body (colony ET430) for phosphatidylinositol imaging W_ET430_1 Reticulitermes speratus 60591 2020-06-10 W_ET430_1 Worker whole body Feed non-labelled cellulose SAMD00532494 Worker whole body (colony ET430) for phosphatidylinositol imaging Sample collection W_ET430_1 Preparation SCEM in a cryo mold frozen at -20 degreeC cryostat (CM1950; Leica Biosystems, Wetzlar, Germany) 10 micrometer Mass spectrometry positive and negative 10-1000 Xevo G2-XS Q-TOF Waters Corporation electrospray ionization quadrupole time of flight 98:2 methanol/water, v/v phosphatidylinositol 100 micrometer 100 micrometer 20000 RAW MassLynx (Waters Corporation) 4.1 W_ET430_1 raw/figure_2e_2g_2i_2k.raw/ Histology Data processing HD Imaging (Waters Corporation) 1.4 Metabolite identification Worker whole body Feed non-labelled cellulose Worker whole body (colony 190501A) for phosphatidylinositol imaging W_190501A_1 Reticulitermes speratus 60591 2021-02-11 W_190501A_1 Worker whole body Feed 13C-labelled cellulose SAMD00532495 Worker whole body (colony 190501A) for phosphatidylinositol imaging Sample collection W_190501A_1 Preparation SCEM in a cryo mold frozen at -20 degreeC cryostat (CM1950; Leica Biosystems, Wetzlar, Germany) 10 micrometer Mass spectrometry positive and negative 10-1000 Xevo G2-XS Q-TOF Waters Corporation electrospray ionization quadrupole time of flight 98:2 methanol/water, v/v phosphatidylinositol 100 micrometer 100 micrometer 20000 RAW MassLynx (Waters Corporation) 4.1 W_190501A_1 raw/figure_2f_2j.raw/ Histology Data processing HD Imaging (Waters Corporation) 1.4 Metabolite identification Worker whole body Feed 13C-labelled cellulose Secondary queen whole body (colony ET430) for phosphatidylinositol imaging SQ_ET430_1 Reticulitermes speratus 60591 2020-06-10 SQ_ET430_1 female Secondary queen whole body Feed non-labelled cellulose SAMD00532496 Secondary queen whole body (colony ET430) for phosphatidylinositol imaging Sample collection SQ_ET430_1 Preparation SCEM in a cryo mold frozen at -20 degreeC cryostat (CM1950; Leica Biosystems, Wetzlar, Germany) 10 micrometer Mass spectrometry positive and negative 10-1000 Xevo G2-XS Q-TOF Waters Corporation electrospray ionization quadrupole time of flight 98:2 methanol/water, v/v phosphatidylinositol 100 micrometer 100 micrometer 20000 RAW MassLynx (Waters Corporation) 4.1 SQ_ET430_1 raw/figure_2e_2g_2i_2k.raw/ Histology Data processing HD Imaging (Waters Corporation) 1.4 Metabolite identification Secondary queen whole body Feed non-labelled cellulose Secondary queen whole body (colony 190501A) for phosphatidylinositol imaging SQ_190501A Reticulitermes speratus 60591 2020-08-18 SQ_190501A female Secondary queen whole body Feed 13C-labelled cellulose SAMD00532497 Secondary queen whole body (colony 190501A) for phosphatidylinositol imaging Sample collection SQ_190501A Preparation SCEM in a cryo mold frozen at -20 degreeC cryostat (CM1950; Leica Biosystems, Wetzlar, Germany) 10 micrometer Mass spectrometry positive and negative 10-1000 Xevo G2-XS Q-TOF Waters Corporation electrospray ionization quadrupole time of flight 98:2 methanol/water, v/v phosphatidylinositol 100 micrometer 100 micrometer 20000 RAW MassLynx (Waters Corporation) 4.1 SQ_190501A raw/figure_2h_2l.raw/ Histology Data processing HD Imaging (Waters Corporation) 1.4 Metabolite identification Secondary queen whole body Feed 13C-labelled cellulose Worker whole body (colony ET430) for acetyl-L-carnitine imaging W_ET430_2 Reticulitermes speratus 60591 2019-10-25 W_ET430_2 Worker whole body Feed non-labelled cellulose SAMD00532502 Worker whole body (colony ET430) for acetyl-L-carnitine imaging Sample collection W_ET430_2 Preparation SCEM in a cryo mold frozen at -20 degreeC cryostat (CM1950; Leica Biosystems, Wetzlar, Germany) 10 micrometer Mass spectrometry positive and negative 10-1000 Xevo G2-XS Q-TOF Waters Corporation electrospray ionization quadrupole time of flight 98:2 methanol/water, v/v acetyl-L-carnitine 100 micrometer 100 micrometer 20000 RAW MassLynx (Waters Corporation) 4.1 W_ET430_2 raw/figure_3f_3j.raw/ Histology Data processing HD Imaging (Waters Corporation) 1.4 Metabolite identification Worker whole body Feed non-labelled cellulose Worker whole body (colony 190501A) for acetyl-L-carnitine imaging W_190501A_4 Reticulitermes speratus 60591 2021-01-20 W_190501A_4 Worker whole body Feed 13C-labelled cellulose SAMD00532503 Worker whole body (colony 190501A) for acetyl-L-carnitine imaging Sample collection W_190501A_4 Preparation SCEM in a cryo mold frozen at -20 degreeC cryostat (CM1950; Leica Biosystems, Wetzlar, Germany) 10 micrometer Mass spectrometry positive and negative 10-1000 Xevo G2-XS Q-TOF Waters Corporation electrospray ionization quadrupole time of flight 98:2 methanol/water, v/v acetyl-L-carnitine 100 micrometer 100 micrometer 20000 RAW MassLynx (Waters Corporation) 4.1 W_190501A_4 raw/figure_3g_3k.raw/ Histology Data processing HD Imaging (Waters Corporation) 1.4 Metabolite identification Worker whole body Feed 13C-labelled cellulose Secondary queen (colony ET430) for acetyl-L-carnitine imaging SQ_ET430_2 Reticulitermes speratus 60591 2020-07-11 SQ_ET430_2 female Worker whole body Feed non-labelled cellulose SAMD00532504 Secondary queen (colony ET430) for acetyl-L-carnitine imaging Sample collection SQ_ET430_2 Preparation SCEM in a cryo mold frozen at -20 degreeC cryostat (CM1950; Leica Biosystems, Wetzlar, Germany) 10 micrometer Mass spectrometry positive and negative 10-1000 Xevo G2-XS Q-TOF Waters Corporation electrospray ionization quadrupole time of flight 98:2 methanol/water, v/v acetyl-L-carnitine 100 micrometer 100 micrometer 20000 RAW MassLynx (Waters Corporation) 4.1 SQ_ET430_2 raw/figure_3h_3l.raw/ Histology Data processing HD Imaging (Waters Corporation) 1.4 Metabolite identification Worker whole body Feed non-labelled cellulose Secondary queen (colony 190514B) for acetyl-L-carnitine imaging SQ_190514B Reticulitermes speratus 60591 2020-08-18 SQ_190514B female Worker whole body Feed 13C-labelled cellulose SAMD00532505 Secondary queen (colony 190514B) for acetyl-L-carnitine imaging Sample collection SQ_190514B Preparation SCEM in a cryo mold frozen at -20 degreeC cryostat (CM1950; Leica Biosystems, Wetzlar, Germany) 10 micrometer Mass spectrometry positive and negative 10-1000 Xevo G2-XS Q-TOF Waters Corporation electrospray ionization quadrupole time of flight 98:2 methanol/water, v/v acetyl-L-carnitine 100 micrometer 100 micrometer 20000 RAW MassLynx (Waters Corporation) 4.1 SQ_190514B raw/figure_3i_3m.raw/ Histology Data processing HD Imaging (Waters Corporation) 1.4 Metabolite identification Worker whole body Feed 13C-labelled cellulose