MAGE-TAB Version	1.1
Comment[MetaboBank accession]	MTBKS211
Study Title	Tracing with 13C-labelled cellulose
Study Description	To confirm whether carbon derived from cellulose ingested by workers was transferred to acetyl-L-carnitine in the worker's body.

Experimental Design	compound treatment design
Experimental Factor Name	treatment
Experimental Factor Type	treatment

Person Last Name	Mitaka	Sakamoto
Person First Name	Yuki	Takumi
Person Mid Initials
Person Affiliation	Kyoto University	Hamamatsu University School of Medicine
Person Roles	submitter	submitter

PubMed ID
Publication DOI	10.1093/pnasnexus/pgad222

Protocol Name	Sample collection	Extraction	Direct infusion	Mass spectrometry	Data processing	Metabolite identification
Protocol Type	Sample collection	Extraction	Direct infusion	Mass spectrometry	Data processing	Metabolite identification
Protocol Description	The digestive tract of the termite individual was removed under a microscope into phosphate-buffered saline and the midgut and hindgut were separated using forceps. During separation, the midgut side of the resection surface was held between the forceps to prevent the release of midgut contents into the PBS. Next, the resection surface of the midgut was dipped into the buffer for chemical analyses, and the contents were collected by gently pressing the midgut with forceps.	The collected midgut contents were extracted in 500 µL of methanol. Then the whole sample was sonicated and centrifuged at 10,000 × g for 5 min at room temperature. The supernatant was collected and adjusted by 50% methanol add 0.1% formic acid.	Extract sample was analysed using directly infusion (DI) in the quadrupole Time of Flight (Q-TOF) mass spectrometer coupled to an electrospray ionization (ESI) source (TripleTOF 5600+, AB Sciex Ltd., USA). Sample was injected with a flow rate of 1 µL/min for a total run time of 10 minute.	MS/MS spectra in the mass range of m/z 50-1,250 were obtained in positive ion mode. Collision Energy (CE):20.0 V was used in positive ion mode for MS/MS fragmentation of ALC.	Data not submitted.	Data not submitted.
Protocol Parameters		Post extraction;Derivatization	DI instrument	Scan polarity;Scan m/z range;Instrument;Ion source;Mass analyzer
Protocol Hardware
Protocol Software

Public Release Date	2023-07-06

Term Source Name
Term Source File
Term Source Version

SDRF File	MTBKS211.sdrf.txt

Comment[Study type]	metabolite target analysis
Comment[Experiment type]	direct infusion-mass spectrometry
Comment[Submission type]	DI-MS
Comment[BioProject]	PRJDB14286
Comment[Related study]
Comment[Contributor]	Takumi Sakamoto
Comment[Submission Date]	2022-10-01
Comment[Last Update Date]	2023-07-06