Source Name	Characteristics[Sample Name]	Characteristics[organism]	Characteristics[taxonomy_id]	Characteristics[collection_date]	Characteristics[geo_loc_name]	Characteristics[strain]	Characteristics[age]	Characteristics[biological_replicate]	Characteristics[sex]	Characteristics[tissue]	Characteristics[treatment]	Comment[BioSample]	Comment[sample_title]	Protocol REF	Sample Name	Protocol REF	Parameter Value[Post extraction]	Parameter Value[Derivatization]	Extract Name	Protocol REF	Parameter Value[Chromatography instrument]	Parameter Value[Autosampler model]	Parameter Value[Column model]	Parameter Value[Column type]	Parameter Value[Guard column]	Labeled Extract Name	Label	Protocol REF	Parameter Value[Scan polarity]	Parameter Value[Scan m/z range]	Parameter Value[Instrument]	Parameter Value[Ion source]	Parameter Value[Mass analyzer]	Assay Name	Comment[technical_replicate]	Raw Data File	Protocol REF	Processed Data File	Protocol REF	Metabolite Assignment File	Comment[maf_value_unit]	Factor Value[diet]
01_01	01_01	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate1	female	Whole body	Complete holidic medium for 1 week from day2	SAMD00646228	CantonS_Ctrl_diet_1	Sample collection	01_01	Extraction	100 microL ultra pure water		01_01	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_01		raw/34__MC_WB1_034.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	Ctrl
01_02	01_02	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate2	female	Whole body	Complete holidic medium for 1 week from day2	SAMD00646229	CantonS_Ctrl_diet_2	Sample collection	01_02	Extraction	100 microL ultra pure water		01_02	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_02		raw/35__MC_WB2_035.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	Ctrl
01_03	01_03	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate3	female	Whole body	Complete holidic medium for 1 week from day2	SAMD00646230	CantonS_Ctrl_diet_3	Sample collection	01_03	Extraction	100 microL ultra pure water		01_03	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_03		raw/36__MC_WB3_036.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	Ctrl
01_04	01_04	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate4	female	Whole body	Complete holidic medium for 1 week from day2	SAMD00646231	CantonS_Ctrl_diet_4	Sample collection	01_04	Extraction	100 microL ultra pure water		01_04	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_04		raw/37__MC_WB4_037.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	Ctrl
01_05	01_05	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate5	female	Whole body	Complete holidic medium for 1 week from day2	SAMD00646232	CantonS_Ctrl_diet_5	Sample collection	01_05	Extraction	100 microL ultra pure water		01_05	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_05		raw/38__MC_WB5_038.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	Ctrl
01_06	01_06	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate6	female	Whole body	Complete holidic medium for 1 week from day2	SAMD00646233	CantonS_Ctrl_diet_6	Sample collection	01_06	Extraction	100 microL ultra pure water		01_06	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_06		raw/39__MC_WB6_039.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	Ctrl
01_07	01_07	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate1	female	Whole body	Methionine restricted holidic medium for 1 week from day2	SAMD00646234	CantonS_MetR_diet_1	Sample collection	01_07	Extraction	100 microL ultra pure water		01_07	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_07		raw/40__MR_WB1_040.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	MetR
01_08	01_08	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate2	female	Whole body	Methionine restricted holidic medium for 1 week from day2	SAMD00646235	CantonS_MetR_diet_2	Sample collection	01_08	Extraction	100 microL ultra pure water		01_08	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_08		raw/41__MR_WB2_041.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	MetR
01_09	01_09	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate3	female	Whole body	Methionine restricted holidic medium for 1 week from day2	SAMD00646236	CantonS_MetR_diet_3	Sample collection	01_09	Extraction	100 microL ultra pure water		01_09	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_09		raw/42__MR_WB3_042.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	MetR
01_10	01_10	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate4	female	Whole body	Methionine restricted holidic medium for 1 week from day2	SAMD00646237	CantonS_MetR_diet_4	Sample collection	01_10	Extraction	100 microL ultra pure water		01_10	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_10		raw/43__MR_WB4_043.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	MetR
01_11	01_11	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate5	female	Whole body	Methionine restricted holidic medium for 1 week from day2	SAMD00646238	CantonS_MetR_diet_5	Sample collection	01_11	Extraction	100 microL ultra pure water		01_11	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_11		raw/44__MR_WB5_044.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	MetR
01_12	01_12	Drosophila melanogaster	7227	2022-02-18	Japan:Hyogo, Kobe, RIKEN	Canton-S	day9	biological_replicate6	female	Whole body	Methionine restricted holidic medium for 1 week from day2	SAMD00646239	CantonS_MetR_diet_6	Sample collection	01_12	Extraction	100 microL ultra pure water		01_12	Chromatography	LC-2040C Plus (Shimadzu)	LC-2040C Plus (Shimadzu)	Discovery HS F5 (2.1 mm x 150 mm, 3 micrometer), Sigma-Aldrich	reverse phase (PFPP)				Mass spectrometry	positive and negative	use MRM method	LCMS-8050 (Shimadzu)	heated electrospray ionization	triple quadrupole	01_12		raw/45__MR_WB6_045.lcd	Data processing	processed/220218_MCMR_head_whole metabolite_Zn_restriction_whole_head_metabolite2.txt	Metabolite identification	MTBKS226.maf.txt	micro mole	MetR