<?xml version="1.0" ?>
<wwPDB-validation-information xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="http://wwpdb.org/validation/schema/wwpdb_validation_v6.06.xsd">
	<Entry percentilebins="all,nmr" attemptedValidationSteps="mogul,buster-report,molprobity,validation-pack,coreclust,chemicalshiftanalysis,percentiles,writexml" PDB-deposition-date="2022-11-26" PDB-revision-date="2023-11-15" PDB-revision-number="3" protein-DNA-RNA-entities="1,2" PDB-resolution="NotAvailable" PDB-resolution-low="NotAvailable" PDB-R="NotAvailable" PDB-Rfree="NotAvailable" pdbid="8BSS" extended_pdbid="8BSS" no-ligands-for-buster-report="yes" percent-rota-outliers-full-length="10.49" bonds_rmsz="0.84" num-H-reduce="22658" clashscore-full-length="0.31" percent-rama-outliers-full-length="2.14" num_bonds_rmsz="22360" angles_rmsz="1.40" RestypesNotcheckedForBondAngleGeometry="LE1,DAB,HYP,4FO" num_angles_rmsz="29940" cyrange_error="success" medoid_model="3" nmrclust_number_of_clusters="3" rci_version="v_1n_11_5_13_A (Berjanski et al., 2005)" nmrclust_number_of_outliers="3" shiftchecker_version="v1.2" nmrclust_version="Kelley et al. (1996)" nmrclust_error="success" chemical_shift_completeness_full_length="91.59" chemical_shift_completeness="91.62" cyrange_version="Kirchner and G\&quot;{u}ntert (2011)" panav_version="Wang et al. (2010)" cyrange_number_of_domains="1" nmr_models_consistency_flag="True" nmrclust_representative_model="18" nmrclust_number_of_models="20" clashscore="0.09" percent-rama-outliers="1.22" percent-rota-outliers="9.44" absolute-percentile-clashscore="98.6" numPDBids-absolute-percentile-clashscore="229148" relative-percentile-clashscore="91.2" numPDBids-relative-percentile-clashscore="14424" absolute-percentile-percent-rama-outliers="12.8" numPDBids-absolute-percentile-percent-rama-outliers="224038" relative-percentile-percent-rama-outliers="61.4" numPDBids-relative-percentile-percent-rama-outliers="12848" absolute-percentile-percent-rota-outliers="10.4" numPDBids-absolute-percentile-percent-rota-outliers="223484" relative-percentile-percent-rota-outliers="55.8" numPDBids-relative-percentile-percent-rota-outliers="12823" XMLcreationDate="Mar 12, 2026 -- 07:50 AM UTC" bmrb_id="34773">
		<chemical_shift_list file_id="5" block_name="starch_output" list_id="1" file_name="working_cs.cif" type="full" total_number_of_shifts="1802" number_of_parsed_shifts="1802" number_of_unparsed_shifts="0" number_of_mapped_shifts="1802" number_of_errors_while_mapping="0" number_of_warnings_while_mapping="0">
			<random_coil_index chain="A" resnum="100" rescode="ASP" value="0.035"/>
			<random_coil_index chain="A" resnum="101" rescode="ASN" value="0.039"/>
			<random_coil_index chain="A" resnum="102" rescode="GLY" value="0.042"/>
			<random_coil_index chain="A" resnum="103" rescode="LYS" value="0.039"/>
			<random_coil_index chain="A" resnum="104" rescode="PRO" value="0.033"/>
			<random_coil_index chain="A" resnum="105" rescode="VAL" value="0.026"/>
			<random_coil_index chain="A" resnum="106" rescode="GLU" value="0.021"/>
			<random_coil_index chain="A" resnum="107" rescode="GLY" value="0.019"/>
			<random_coil_index chain="A" resnum="108" rescode="HIS" value="0.020"/>
			<random_coil_index chain="A" resnum="109" rescode="ALA" value="0.021"/>
			<random_coil_index chain="A" resnum="110" rescode="SER" value="0.019"/>
			<random_coil_index chain="A" resnum="111" rescode="GLN" value="0.016"/>
			<random_coil_index chain="A" resnum="112" rescode="MET" value="0.015"/>
			<random_coil_index chain="A" resnum="113" rescode="HIS" value="0.015"/>
			<random_coil_index chain="A" resnum="114" rescode="TYR" value="0.015"/>
			<random_coil_index chain="A" resnum="115" rescode="GLU" value="0.019"/>
			<random_coil_index chain="A" resnum="116" rescode="LEU" value="0.020"/>
			<random_coil_index chain="A" resnum="117" rescode="ALA" value="0.023"/>
			<random_coil_index chain="A" resnum="118" rescode="LYS" value="0.021"/>
			<random_coil_index chain="A" resnum="119" rescode="ASP" value="0.019"/>
			<random_coil_index chain="A" resnum="120" rescode="PHE" value="0.015"/>
			<random_coil_index chain="A" resnum="121" rescode="VAL" value="0.014"/>
			<random_coil_index chain="A" resnum="122" rescode="VAL" value="0.017"/>
			<random_coil_index chain="A" resnum="123" rescode="LEU" value="0.019"/>
			<random_coil_index chain="A" resnum="124" rescode="THR" value="0.023"/>
			<random_coil_index chain="A" resnum="125" rescode="GLY" value="0.025"/>
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			<random_coil_index chain="A" resnum="127" rescode="ALA" value="0.024"/>
			<random_coil_index chain="A" resnum="128" rescode="TYR" value="0.020"/>
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			<random_coil_index chain="A" resnum="130" rescode="GLN" value="0.021"/>
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			<random_coil_index chain="A" resnum="132" rescode="VAL" value="0.031"/>
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			<random_coil_index chain="A" resnum="136" rescode="ILE" value="0.019"/>
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			<random_coil_index chain="A" resnum="154" rescode="SER" value="0.028"/>
			<random_coil_index chain="A" resnum="155" rescode="ASP" value="0.055"/>
			<random_coil_index chain="A" resnum="156" rescode="LYS" value="0.144"/>
			<random_coil_index chain="A" resnum="157" rescode="GLY" value="0.291"/>
			<random_coil_index chain="A" resnum="158" rescode="LYS" value="0.429"/>
			<random_coil_index chain="A" resnum="159" rescode="ARG" value="0.499"/>
			<random_coil_index chain="A" resnum="28" rescode="VAL" value="0.290"/>
			<random_coil_index chain="A" resnum="29" rescode="THR" value="0.227"/>
			<random_coil_index chain="A" resnum="30" rescode="GLY" value="0.152"/>
			<random_coil_index chain="A" resnum="31" rescode="ASP" value="0.085"/>
			<random_coil_index chain="A" resnum="32" rescode="THR" value="0.077"/>
			<random_coil_index chain="A" resnum="33" rescode="ASP" value="0.067"/>
			<random_coil_index chain="A" resnum="34" rescode="GLN" value="0.073"/>
			<random_coil_index chain="A" resnum="35" rescode="PRO" value="0.065"/>
			<random_coil_index chain="A" resnum="36" rescode="ILE" value="0.056"/>
			<random_coil_index chain="A" resnum="37" rescode="HIS" value="0.036"/>
			<random_coil_index chain="A" resnum="38" rescode="ILE" value="0.024"/>
			<random_coil_index chain="A" resnum="39" rescode="GLU" value="0.020"/>
			<random_coil_index chain="A" resnum="40" rescode="SER" value="0.022"/>
			<random_coil_index chain="A" resnum="41" rescode="ASP" value="0.022"/>
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			<random_coil_index chain="A" resnum="43" rescode="GLN" value="0.031"/>
			<random_coil_index chain="A" resnum="44" rescode="SER" value="0.049"/>
			<random_coil_index chain="A" resnum="45" rescode="LEU" value="0.063"/>
			<random_coil_index chain="A" resnum="46" rescode="ASP" value="0.073"/>
			<random_coil_index chain="A" resnum="47" rescode="MET" value="0.071"/>
			<random_coil_index chain="A" resnum="48" rescode="GLN" value="0.066"/>
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			<random_coil_index chain="A" resnum="53" rescode="THR" value="0.023"/>
			<random_coil_index chain="A" resnum="54" rescode="PHE" value="0.023"/>
			<random_coil_index chain="A" resnum="55" rescode="THR" value="0.023"/>
			<random_coil_index chain="A" resnum="56" rescode="GLY" value="0.027"/>
			<random_coil_index chain="A" resnum="57" rescode="ASN" value="0.031"/>
			<random_coil_index chain="A" resnum="58" rescode="VAL" value="0.032"/>
			<random_coil_index chain="A" resnum="59" rescode="ILE" value="0.026"/>
			<random_coil_index chain="A" resnum="60" rescode="VAL" value="0.020"/>
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			<random_coil_index chain="A" resnum="67" rescode="ILE" value="0.015"/>
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			<random_coil_index chain="A" resnum="70" rescode="ASP" value="0.016"/>
			<random_coil_index chain="A" resnum="71" rescode="LYS" value="0.014"/>
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			<random_coil_index chain="A" resnum="75" rescode="THR" value="0.024"/>
			<random_coil_index chain="A" resnum="76" rescode="ARG" value="0.032"/>
			<random_coil_index chain="A" resnum="77" rescode="PRO" value="0.044"/>
			<random_coil_index chain="A" resnum="78" rescode="GLY" value="0.049"/>
			<random_coil_index chain="A" resnum="79" rescode="GLY" value="0.057"/>
			<random_coil_index chain="A" resnum="80" rescode="GLU" value="0.058"/>
			<random_coil_index chain="A" resnum="81" rescode="GLN" value="0.057"/>
			<random_coil_index chain="A" resnum="82" rescode="GLY" value="0.047"/>
			<random_coil_index chain="A" resnum="83" rescode="LYS" value="0.036"/>
			<random_coil_index chain="A" resnum="84" rescode="GLU" value="0.028"/>
			<random_coil_index chain="A" resnum="85" rescode="VAL" value="0.023"/>
			<random_coil_index chain="A" resnum="86" rescode="ILE" value="0.019"/>
			<random_coil_index chain="A" resnum="87" rescode="ASP" value="0.016"/>
			<random_coil_index chain="A" resnum="88" rescode="GLY" value="0.014"/>
			<random_coil_index chain="A" resnum="89" rescode="TYR" value="0.016"/>
			<random_coil_index chain="A" resnum="90" rescode="GLY" value="0.022"/>
			<random_coil_index chain="A" resnum="91" rescode="LYS" value="0.030"/>
			<random_coil_index chain="A" resnum="92" rescode="PRO" value="0.033"/>
			<random_coil_index chain="A" resnum="93" rescode="ALA" value="0.029"/>
			<random_coil_index chain="A" resnum="94" rescode="THR" value="0.021"/>
			<random_coil_index chain="A" resnum="95" rescode="PHE" value="0.018"/>
			<random_coil_index chain="A" resnum="96" rescode="TYR" value="0.018"/>
			<random_coil_index chain="A" resnum="97" rescode="GLN" value="0.025"/>
			<random_coil_index chain="A" resnum="98" rescode="MET" value="0.030"/>
			<random_coil_index chain="A" resnum="99" rescode="GLN" value="0.034"/>
			<random_coil_index chain="B" resnum="206" rescode="VAL" value="0.031"/>
			<random_coil_index chain="B" resnum="208" rescode="ILE" value="0.019"/>
			<random_coil_index chain="B" resnum="209" rescode="ILE" value="0.012"/>
			<random_coil_index chain="B" resnum="210" rescode="TYR" value="0.011"/>
			<random_coil_index chain="B" resnum="212" rescode="ASN" value="0.052"/>
			<random_coil_index chain="B" resnum="213" rescode="ARG" value="0.079"/>
			<random_coil_index chain="B" resnum="215" rescode="THR" value="0.262"/>
			<random_coil_index chain="B" resnum="217" rescode="LYS" value="0.090"/>
			<random_coil_index chain="B" resnum="218" rescode="CYS" value="0.008"/>
			<random_coil_index chain="B" resnum="221" rescode="TYR" value="0.163"/>
			<referencing_offset atom="C" number_of_measurements="129" value="0.38" uncertainty="0.50" precision="0.09"/>
			<referencing_offset atom="CA" number_of_measurements="132" value="-0.08" uncertainty="0.50" precision="0.12"/>
			<referencing_offset atom="CB" number_of_measurements="118" value="-0.32" uncertainty="0.50" precision="0.10"/>
			<referencing_offset atom="N" number_of_measurements="126" value="-0.60" uncertainty="0.50" precision="0.27"/>
			<assignment_completeness_well_defined type="stereomethyl" element="stereomethyl" number_of_assigned_shifts="0" number_of_unassigned_shifts="17" number_of_shifts="17"/>
			<assignment_completeness_well_defined element="Total" type="overall" number_of_assigned_shifts="1389" number_of_unassigned_shifts="127" number_of_shifts="1516"/>
			<assignment_completeness_well_defined element="Total" type="backbone" number_of_assigned_shifts="533" number_of_unassigned_shifts="30" number_of_shifts="563"/>
			<assignment_completeness_well_defined element="Total" type="sidechain" number_of_assigned_shifts="762" number_of_unassigned_shifts="77" number_of_shifts="839"/>
			<assignment_completeness_well_defined element="Total" type="aromatic" number_of_assigned_shifts="94" number_of_unassigned_shifts="20" number_of_shifts="114"/>
			<assignment_completeness_well_defined element="H" type="overall" number_of_assigned_shifts="817" number_of_unassigned_shifts="13" number_of_shifts="830"/>
			<assignment_completeness_well_defined element="H" type="backbone" number_of_assigned_shifts="230" number_of_unassigned_shifts="0" number_of_shifts="230"/>
			<assignment_completeness_well_defined element="H" type="sidechain" number_of_assigned_shifts="535" number_of_unassigned_shifts="10" number_of_shifts="545"/>
			<assignment_completeness_well_defined element="H" type="aromatic" number_of_assigned_shifts="52" number_of_unassigned_shifts="3" number_of_shifts="55"/>
			<assignment_completeness_well_defined element="C" type="overall" number_of_assigned_shifts="454" number_of_unassigned_shifts="90" number_of_shifts="544"/>
			<assignment_completeness_well_defined element="C" type="backbone" number_of_assigned_shifts="203" number_of_unassigned_shifts="21" number_of_shifts="224"/>
			<assignment_completeness_well_defined element="C" type="sidechain" number_of_assigned_shifts="212" number_of_unassigned_shifts="52" number_of_shifts="264"/>
			<assignment_completeness_well_defined element="C" type="aromatic" number_of_assigned_shifts="39" number_of_unassigned_shifts="17" number_of_shifts="56"/>
			<assignment_completeness_well_defined element="N" type="overall" number_of_assigned_shifts="118" number_of_unassigned_shifts="24" number_of_shifts="142"/>
			<assignment_completeness_well_defined element="N" type="backbone" number_of_assigned_shifts="100" number_of_unassigned_shifts="9" number_of_shifts="109"/>
			<assignment_completeness_well_defined element="N" type="sidechain" number_of_assigned_shifts="15" number_of_unassigned_shifts="15" number_of_shifts="30"/>
			<assignment_completeness_well_defined element="N" type="aromatic" number_of_assigned_shifts="3" number_of_unassigned_shifts="0" number_of_shifts="3"/>
			<assignment_completeness_full_length type="stereomethyl" element="stereomethyl" number_of_assigned_shifts="0" number_of_unassigned_shifts="21" number_of_shifts="21"/>
			<assignment_completeness_full_length element="Total" type="overall" number_of_assigned_shifts="1742" number_of_unassigned_shifts="160" number_of_shifts="1902"/>
			<assignment_completeness_full_length element="Total" type="backbone" number_of_assigned_shifts="677" number_of_unassigned_shifts="39" number_of_shifts="716"/>
			<assignment_completeness_full_length element="Total" type="sidechain" number_of_assigned_shifts="961" number_of_unassigned_shifts="101" number_of_shifts="1062"/>
			<assignment_completeness_full_length element="Total" type="aromatic" number_of_assigned_shifts="104" number_of_unassigned_shifts="20" number_of_shifts="124"/>
			<assignment_completeness_full_length element="H" type="overall" number_of_assigned_shifts="1019" number_of_unassigned_shifts="21" number_of_shifts="1040"/>
			<assignment_completeness_full_length element="H" type="backbone" number_of_assigned_shifts="290" number_of_unassigned_shifts="4" number_of_shifts="294"/>
			<assignment_completeness_full_length element="H" type="sidechain" number_of_assigned_shifts="672" number_of_unassigned_shifts="14" number_of_shifts="686"/>
			<assignment_completeness_full_length element="H" type="aromatic" number_of_assigned_shifts="57" number_of_unassigned_shifts="3" number_of_shifts="60"/>
			<assignment_completeness_full_length element="C" type="overall" number_of_assigned_shifts="576" number_of_unassigned_shifts="104" number_of_shifts="680"/>
			<assignment_completeness_full_length element="C" type="backbone" number_of_assigned_shifts="261" number_of_unassigned_shifts="23" number_of_shifts="284"/>
			<assignment_completeness_full_length element="C" type="sidechain" number_of_assigned_shifts="271" number_of_unassigned_shifts="64" number_of_shifts="335"/>
			<assignment_completeness_full_length element="C" type="aromatic" number_of_assigned_shifts="44" number_of_unassigned_shifts="17" number_of_shifts="61"/>
			<assignment_completeness_full_length element="N" type="overall" number_of_assigned_shifts="147" number_of_unassigned_shifts="35" number_of_shifts="182"/>
			<assignment_completeness_full_length element="N" type="backbone" number_of_assigned_shifts="126" number_of_unassigned_shifts="12" number_of_shifts="138"/>
			<assignment_completeness_full_length element="N" type="sidechain" number_of_assigned_shifts="18" number_of_unassigned_shifts="23" number_of_shifts="41"/>
			<assignment_completeness_full_length element="N" type="aromatic" number_of_assigned_shifts="3" number_of_unassigned_shifts="0" number_of_shifts="3"/>
		</chemical_shift_list>
	</Entry>
	<Model nmrclust_cluster_id="3" model="1"/>
	<ModelledSubgroup num-H-reduce="8" rota="t" validate="False" model="1" chain="A" resnum="28" said="A" ent="1" seq="1" icode=" " resname="VAL" altcode=" "/>
	<ModelledSubgroup num-H-reduce="7" phi="-148.1" psi="177.2" rama="Favored" rota="p" validate="False" model="1" chain="A" resnum="29" said="A" ent="1" seq="2" icode=" " resname="THR" altcode=" ">
		<plane-outlier type="peptide" omega="149.27"/>
	</ModelledSubgroup>
	<ModelledSubgroup num-H-reduce="3" phi="-86.8" psi="-149.0" rama="Favored" validate="False" model="1" chain="A" resnum="30" said="A" ent="1" seq="3" icode=" " resname="GLY" altcode=" "/>
	<ModelledSubgroup num-H-reduce="4" phi="-78.5" psi="74.6" rama="Favored" rota="t0" validate="False" model="1" chain="A" resnum="31" said="A" ent="1" seq="4" icode=" " resname="ASP" altcode=" "/>
	<ModelledSubgroup num-H-reduce="7" phi="-146.8" psi="167.2" rama="Favored" rota="p" validate="False" model="1" chain="A" resnum="32" said="A" ent="1" seq="5" icode=" " resname="THR" altcode=" "/>
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	<ModelledEntityInstance bonds_rmsz="0.82" num_bonds_rmsz="85" angles_rmsz="1.25" num_angles_rmsz="104" absolute_sidechain_percentile="1.14" relative_sidechain_percentile="1.14" absolute_rama_percentile="100.00" relative_rama_percentile="100.00" said="B" model="9" chain="B" ent="2"/>
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	<ModelledEntityInstance bonds_rmsz="0.78" num_bonds_rmsz="85" angles_rmsz="1.09" num_angles_rmsz="104" absolute_sidechain_percentile="7.12" relative_sidechain_percentile="7.12" absolute_rama_percentile="100.00" relative_rama_percentile="100.00" said="B" model="19" chain="B" ent="2"/>
	<ModelledEntityInstance bonds_rmsz="0.82" num_bonds_rmsz="85" angles_rmsz="1.13" num_angles_rmsz="104" absolute_sidechain_percentile="100.00" relative_sidechain_percentile="100.00" absolute_rama_percentile="100.00" relative_rama_percentile="100.00" said="B" model="20" chain="B" ent="2"/>
	<programs>
		<program name="validation-pipeline" version="2.49" properties="PDB-revision-number,PDB-resolution,PDB-deposition-date,percentilebins,PDB-Rfree,extended_pdbid,protein-DNA-RNA-entities,pdbid,attemptedValidationSteps,PDB-revision-date,PDB-resolution-low,PDB-R,XMLcreationDate"/>
		<program name="mogul" version="2022.3.0, CSD as543be (2022)" properties="mogul-torsion-outlier,mogul_rmsz_numbonds,mogul-ring-outlier,mogul-bond-outlier,mogul-angle-outlier,mogul_bonds_rmsz,mogul_rmsz_numangles,mogul_angles_rmsz"/>
		<program name="buster-report" version="unknown" properties="no-ligands-for-buster-report"/>
		<program name="molprobity" version="4-5-2 with Phenix2.0" properties="percent-rota-outliers-full-length,clash,angle-outlier,bonds_rmsz,psi,num-H-reduce,relative_sidechain_percentile,clashscore-full-length,percent-rama-outliers-full-length,absolute_rama_percentile,relative_rama_percentile,rama,cis_peptide,phi,num_bonds_rmsz,bond-outlier,rota,angles_rmsz,RestypesNotcheckedForBondAngleGeometry,num_angles_rmsz,absolute_sidechain_percentile"/>
		<program name="validation-pack" version="unknown" properties="plane-outlier"/>
		<program name="coreclust" version="2.49" properties="cyrange_number_of_domains,nmrclust_version,nmrclust_error,medoid_model,cyrange_error,cyrange_domain_id,nmrclust_number_of_clusters,nmrclust_number_of_outliers,nmrclust_representative,nmrclust_representative_model,validate,cyrange_version,nmrclust_number_of_models,nmrclust_cluster_id"/>
		<program name="chemicalshiftanalysis" version="v1.2" properties="chemical_shift_completeness,shiftchecker_version,panav_version,rci_version,chemical_shift_completeness_full_length"/>
		<program name="coreclust" version="unknown" properties="nmr_models_consistency_flag"/>
		<program name="nmrwriter" version="2.49" properties="clashscore,percent-rama-outliers,percent-rota-outliers"/>
		<program name="percentiles" version="20250101.v01 (using entries in the PDB archive January 1st 2025)" properties="absolute-percentile-clashscore,numPDBids-absolute-percentile-clashscore,numPDBids-relative-percentile-percent-rota-outliers,absolute-percentile-percent-rama-outliers,numPDBids-relative-percentile-percent-rama-outliers,numPDBids-relative-percentile-clashscore,absolute-percentile-percent-rota-outliers,relative-percentile-percent-rama-outliers,relative-percentile-percent-rota-outliers,relative-percentile-clashscore,numPDBids-absolute-percentile-percent-rota-outliers,numPDBids-absolute-percentile-percent-rama-outliers"/>
	</programs>
</wwPDB-validation-information>
