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<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VMY4</accession>
    <name>A0A133VMY4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000032">
        <property type="protein sequence ID" value="KXB07818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VMY4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11080" checksum="D3B2892254610442" modified="2016-07-06" version="1">MSGGAINKEEIAEFILSHLKEDDFVLDVGCGNGGPTQGAGEAEGRSGFQSSSTRKGRRSPRPPVLVWRKNRRAMGGSWVRVNCDSGSEDSDLRYTQLSLHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VMZ4</accession>
    <name>A0A133VMZ4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000031">
        <property type="protein sequence ID" value="KXB07828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VMZ4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="270" mass="32107" checksum="ACC5513BC8124B9C" modified="2016-07-06" version="1">MIERWGGTMLMNEADFYNSDTTAEMVKILNEGFERSGAVIKAHMERQPEVVATIPFGPKILGTRKRWKDQALESRCITEVMHETEREDILPVLTYKFRERQQKLRNKLLMFRFKNYHKIDEGKIEDLWPEFREMKLDRRLIQATIGFSVLFWQDEEMFKRFKKFLKKQQKELKEERAASFDGGIVKAIYELRDIPHTTPGDIADHMEEEQNYKEVSHQKIGKHLKRLGLKTKAKKIGGKTERAIPNDKKQIQRVFKRYIPDYEIGQEELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN10</accession>
    <name>A0A133VN10_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000032">
        <property type="protein sequence ID" value="KXB07819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN10"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="59" mass="6968" checksum="DCB942AC6C746933" modified="2016-07-06" version="1">MEDKFESIESEIGETRKKLEDRVKAIEEKIAGLENKIKKLRKSNRRLKKEMASSREGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN18</accession>
    <name>A0A133VN18_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AKJ58_01700</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000032">
        <property type="protein sequence ID" value="KXB07820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN18"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.276"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00427">
        <property type="entry name" value="NAAT family transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB07820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="206" mass="22542" checksum="F4694A1405C45390" modified="2016-07-06" version="1">MNYLDFFLKAFVTIFVIVDPPGNIPLFMSLTESASDEERNTVSKKATLIAAVLLLFIVLTGNFILEVFRITLDGLRIAGGILLFVISIDILMGERRKRKYVEEGRESIDTDTLATFPIALPLYTGPGAITAGIVLYSTAETMIPKLLVILSIVVTYLIVRVTHMYSDKIIGLLGKSGSNIVARLMAIFLAAIAVEYFFAGVMGKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN24</accession>
    <name>A0A133VN24_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfate adenylyltransferase subunit 2</fullName>
            <ecNumber evidence="2">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-sulfurylase small subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Sulfate adenylate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_01630</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAPS reductase family. CysD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="2"/>
    <dbReference type="EMBL" id="LHYK01000029">
        <property type="protein sequence ID" value="KXB07852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN24"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.233"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011784">
        <property type="entry name" value="SO4_adenylTrfase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050128">
        <property type="entry name" value="Sulfate_adenylyltrnsfr_sub2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003587">
        <property type="entry name" value="PRK05253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002936">
        <property type="entry name" value="CysDAde_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB07852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="265" mass="31006" checksum="273ABEFFC2FE1C7E" modified="2016-07-06" version="1">MDHLDRLESESIYIIREAYSKYHKLAMMWSAGKDSTVLAYLTRKAFLGKAPIPAIYIDTGYHFQKMYDFRDKLVDEWDLNLVIAKNEEADKKNVGPEDKLKCCGMRKTQALKDTIAKHGFDALLVGIRRDEHGIRSKERFFSPRDQNLRWNYKDQPPELWDQYKSQREAGQHLRVHPLLSWTELDVWEYIKRENLPVNPLYFAKSGKRYRSLGCKPCTEPVASDVRNVDDMIKEIRKSQVSERSGRAQDKESAHAMQSLRALGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN38</accession>
    <name>A0A133VN38_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000030">
        <property type="protein sequence ID" value="KXB07840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN38"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="159" mass="18641" checksum="5B06774A19A42DA4" modified="2016-07-06" version="1">MSEVLPSRYDYLKLTIWVCNGKKPTEEALDWTPQQEVIKKTPVGKGTKDPVGYVQNYIRRLIETGDVKSKKEKLTSEERWGEEHEIFGNRPLEVKLIKLRQSTIDYIKKVEEKNEMYPFGVGDTYRKHMEKRKKLKGYTGELELKLKRMESVSSATQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN56</accession>
    <name>A0A133VN56_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000030">
        <property type="protein sequence ID" value="KXB07843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN56"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="555" mass="63155" checksum="D507FE988C991F1D" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN59</accession>
    <name>A0A133VN59_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="11">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">moaA</name>
        <name evidence="13" type="ORF">AKJ58_01565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="11"/>
    <dbReference type="EMBL" id="LHYK01000027">
        <property type="protein sequence ID" value="KXB07869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN59"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.218"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001199">
        <property type="entry name" value="PRK00164.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="252"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KXB07869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="303" mass="34200" checksum="C384EC117BFF26A4" modified="2016-07-06" version="1">MRDSYGRPVTGLRISVTNACNLDCFYCHGEGCQEGSREMTSEEIGNLVRFGSELGIKKVKLTGGEPLVRDDIEEIVSEVAQPSIVDVSMTTNGTQLTRKAEALGEAGLDRINVSLDSLDPQIYARIAGKSKLNQVLDGIDAALDADLWPVKLNMIILNGVNDDEVDRMIEYSLEKEVILQLIELLWTPETAEIYERYHRELSFIEQGLEKRASKVRTRWAMQARRKYVLDGGGEVEVVNPMHNSEFCNHCTRLRTTPDGYLKPCLMRNDNLVDFLTPLRSGDKDGVYKAFKEAIARREPYHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN77</accession>
    <name>A0A133VN77_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AKJ58_01600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000028">
        <property type="protein sequence ID" value="KXB07863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN77"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF22">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="39"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KXB07863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="335" mass="39113" checksum="17FBD4D4E70614CE" modified="2016-07-06" version="1">MIRDARVLREYFIPRRILHREGQLEAIRDSLKPVLEGKSPRDMHLYGPPGSGKTCIARYLLEELSKRSSRIRTSYVNCFDNSSKFRVLYEVVRDIGSVLSVHEKGTPTDEVLERVRKLVEDRYCILVLDEVDKLRETDVLYSLARTPEIGLIFISNRETSLYRIDSRIRSSLACMQTLEFPEYSRGKLVDILKDRAKWGLMPESYREEQLERIAMFAEGDARLAIDSLRLAAERSEEGDHEEIQDEFIEGSVSKAKKEIDSKTLEKLNDDQLLLYRILKPEEELRAGELHEKYKEKAEDPVVDRTVRKYLGKLERLGLIETEGEGRWRVYKVRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN83</accession>
    <name>A0A133VN83_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CARDB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_01510</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000024">
        <property type="protein sequence ID" value="KXB07904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN83"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.207"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09136">
        <property type="entry name" value="Glucodextran_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22352">
        <property type="entry name" value="K319L-like_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="517"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="CARDB" evidence="3">
        <location>
            <begin position="423"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB07904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="548" mass="57447" checksum="AD1FCC73C323E188" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN92</accession>
    <name>A0A133VN92_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Carbamoyl phosphate synthase large chain, N-terminal section</fullName>
            <ecNumber evidence="16">6.3.4.16</ecNumber>
            <ecNumber evidence="5">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Carbamoyl phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">AKJ58_01460</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Large subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 27">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="16"/>
    <dbReference type="EC" id="6.3.5.5" evidence="5"/>
    <dbReference type="EMBL" id="LHYK01000023">
        <property type="protein sequence ID" value="KXB07914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN92"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.193"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000013">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="12 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="23">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="23">
        <location>
            <begin position="547"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="24">
        <location>
            <begin position="805"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="25">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KXB07914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="953" mass="105545" checksum="8F2AF71C4A840EF6" modified="2016-07-06" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNA4</accession>
    <name>A0A133VNA4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000022">
        <property type="protein sequence ID" value="KXB07932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNA4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="76" mass="8182" checksum="C1008C25C2F829E7" modified="2016-07-06" version="1">MVEAYVLVTSAIGKVKEVAEKLQDLENVENVRVVTGPFDVVVLAKAKDLSTLTNTVVEGIRRIDGVVNTDTAIVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNB2</accession>
    <name>A0A133VNB2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000022">
        <property type="protein sequence ID" value="KXB07929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNB2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="62" mass="7066" checksum="D672CBAB6D374D67" modified="2016-07-06" version="1">MNLKKIADGLYIGGDKTPRKYNVFDFIRDPWSNNFILSPIVSLKPKPAQLSKTRSELGFLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNB6</accession>
    <name>A0A133VNB6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IrrE N-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000024">
        <property type="protein sequence ID" value="KXB07901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNB6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019504">
        <property type="entry name" value="Peptidase_U49_Lit_pept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10463">
        <property type="entry name" value="Peptidase_U49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14289" checksum="DFE6BAC5C103588F" modified="2016-07-06" version="1">MPYKEEYLDESVIRNPEISELVLEVSEKVLEEEIPPRMIIYASDGVNESHVDEDRVYFSTRDFEKLDRTAGLGLVAHEIAHVCLKHGINKEPKMADEKEADSLATRWGFKEEIEKLRKEFPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNC4</accession>
    <name>A0A133VNC4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000021">
        <property type="protein sequence ID" value="KXB07948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNC4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="73" mass="8330" checksum="8027A0D7E235AF1D" modified="2016-07-06" version="1">MSPFPAVAIFGFPVATFDYIWHSQLHLNQEPLRSQLYIISISHGQDSTEESIEGAIVLIYLAFFFHFGRWQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNC9</accession>
    <name>A0A133VNC9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000020">
        <property type="protein sequence ID" value="KXB07962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNC9"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.155"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="entry name" value="Metalloenzyme domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="376" mass="41458" checksum="A7823C1614714DCA" modified="2016-07-06" version="1" fragment="single">FFQLFPYFSLNEIKPGVRPGSDTAHLSIFGYDPEEVYTGRGFFEALGIGIEMKDGDVSFRTNFATVDENFIVKDRRAGRIKKWQKKLEKALQKLESPQPDVKVIFKASTEHRGALILRGPNLSGQISDIDPHKTGVKVSKAKPLTKDKPSERTARIVNKLVKQSYEILKNLPLNKKREENGKLPANIILPRGAASKPDLISFKEKYGVDGEAIAAGALYIGVARALKLKFKQAEGVTGGADSPIMNKAKLAVKRLNKNVNFVFIHIKGADSCGHDHDAEAKISFIEKIDETIGYLLRNLNWSETHMALTGDHSTPIVYGDHVADPVPIVFVGPNVTPDEVKEFNERSVLKGGVGRISGRAVPVLLGYSNLLEKFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VND2</accession>
    <name>A0A133VND2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000021">
        <property type="protein sequence ID" value="KXB07949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VND2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="156" mass="18009" checksum="24963734AC3C4B58" modified="2016-07-06" version="1">MAKDRQILIRVSPEQKKEYEEFAKEFTEGNMARMFRRCAKIVMKLERNQNDQQQELSPFKSSLDAIFELCETMDRKLSVLSMTRDSGEIDSSVLEVARNILDQMGEMDNPTYSQIFGNTDVEQDVFEGAVFLIESLGWEVRRDDPNDYEGGGENCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VND5</accession>
    <name>A0A133VND5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CTP synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">6.3.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_01340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="3"/>
    <dbReference type="EMBL" id="LHYK01000020">
        <property type="protein sequence ID" value="KXB07958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VND5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.150"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB07958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="160" mass="18222" checksum="FE7270D10F8C29E9" modified="2016-07-06" version="1" fragment="single">GICFGAQLFFIAFCRRMGLNGANSAEIDPEAEHPVVDLLPEQKKISEKGGTMRLGGHEIVLKPGTKLFKAYGKKRVVERFRHRYHLIPEYAHRFEGGGLVISAMDKDRRIINAIEIEGDHWMVGTQFHPEFTSRPEKPSPIYSDFISAALKRKRRESKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNE8</accession>
    <name>A0A133VNE8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AKJ58_01435</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000022">
        <property type="protein sequence ID" value="KXB07931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNE8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05656">
        <property type="entry name" value="M42_Frv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="entry name" value="Peptidase M42, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051464">
        <property type="entry name" value="Peptidase_M42_aminopept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481:SF0">
        <property type="entry name" value="AMINOPEPTIDASE YPDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB07931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="36724" checksum="0F0241F9295B4A31" modified="2016-07-06" version="1">MRELLARLADLYGASGREQDLVSEIREEFKQTADEVSIDKFGNVIATKRGSGDLSVMLAAHMDEIGLAVEKVDGDGFAFPSNVGGWNPQILPSNPLRGNKCWGIASISPLGLLDKEEKKKAIKSEDLFIDGDNFSSGDFLVQDSSTATLGNPNLLVGKSFDDRAGVAVLIKILRELSDDLEIDIYGVATVQEEVGARGAGMAAYKLDPDLGLVLEVALADDVPMNEPHTPVVRLGKGPAITVKDASMISHPGLTNFLIKVAEEREIPHQLEILERGGTDAGRIHISREGVPSSVIGIPTRYLHSPVEVLDLRDLERVVELVTSALEGLTKPKFEAFFQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNF6</accession>
    <name>A0A133VNF6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HNH nuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000019">
        <property type="protein sequence ID" value="KXB07974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNF6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13392">
        <property type="entry name" value="HNH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="1">
        <location>
            <begin position="11"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="135" mass="16046" checksum="DF24C514773FF303" modified="2016-07-06" version="1">MERKEIPRKRILELGARFRNRCEICGRPVRGSNVDIHHIDGNPRNNRLSNLLLVCSGMGAIDCHRQAHQEKYPKQIKFTDKKEKIIDWVAEYAEELESRVDGYKVWKSFSNDYNENRTYEDNKERFKKILESNYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNG2</accession>
    <name>A0A133VNG2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNG2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="138" mass="15235" checksum="FF98324F159C9C5D" modified="2016-07-06" version="1">MTPLAQKVGNHQVEGTQRDVSGRTDGQATDDEETGHSSTQIESEVQQLPVHFSGESGPMQKLFVLPLNPLGFQPQFLSRTFRGTGVGVFVASPRLGEADENSGPFPALFAYLRYLEISAFKYVSSDHELFAPMKITWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNG5</accession>
    <name>A0A133VNG5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uracil-DNA glycosylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNG5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="1">
        <location>
            <begin position="86"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="213" mass="25181" checksum="74E735B87164E3C2" modified="2016-07-06" version="1">MFQNKRKLKSLLKEMWECSECSDSERESEKSYGQINVDLSETWAKNFKRSTFLKGPWIFPPYKVQNDKGEMKLVRGFFGKGGDKGIMFIAERPSTGKFPDKRCRMFYELLAKNNLHDAHLTDFVKCRGLVREGVKKVKENCFERILKREIEIVAPKIVIPIGNEAERLCKQYLVEDILEEKKLKEKIIHYAYRFDSKENVKKEIQGRLEKFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNH1</accession>
    <name>A0A133VNH1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="LHYK01000020">
        <property type="protein sequence ID" value="KXB07961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNH1"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.154"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000006">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="245"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB07961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="340" mass="36431" checksum="D02D801581A03312" modified="2016-07-06" version="1">MRTIQLKNDYIELIDQTVLPKRLEFVRCQNAEEVAQAIEQMKIRGAPALGAAAGMALAVTALHSRASDSRVMLRELKAATNRIKRTRPTAANLFMALNRVMKVAEKSVTGSVKELQKAVVEEAKAIAEEDVKINREMGRQGAQLIQEGNTILTHCNAGALACVDYGTALGVIRAARGQGKGVSVIATETRPLFQGSRLTTWELKHDGIPVTLITDGAVGHTMSEGNVDLVIVGADRIAGNGDVANKIGTYSIAVLANRYDIPFYVAAPTSTIDPQTRSGKEIEIEYRDPSEVTSLGGHKLAPDGIDVSNPAFDVTPAGLITKIITENGVSEPSELESLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNH2</accession>
    <name>A0A133VNH2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNH2"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.124"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058782">
        <property type="entry name" value="GIY_YIG_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26468">
        <property type="entry name" value="GIY_YIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="233" mass="26905" checksum="2E38CF7A66DEB1E2" modified="2016-07-06" version="1">MSRKEDVDRLYEILNELENAVGGKRKLENCDGRMDWPRRGVYFFFDPREERKSTDELRVTRVGTHAVSRGSETTLWDRLKTHRGTLSGKYAGGGNHRGSVFRKEVGRAIIAREGGEQKYPDWGEGSSAEPEVRKRELDMEKEVSKHIRNLPFLWIKINDKPGAGSKRAFVERNSIALLSNFGKEPIDPREQAWLGNHSPKRKIRESGLWNVDYVDESYKRGFLEEMRGIVRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNH8</accession>
    <name>A0A133VNH8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11 12">Histidinol dehydrogenase</fullName>
            <shortName evidence="11 12">HDH</shortName>
            <ecNumber evidence="4 11 12">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hisD</name>
        <name evidence="17" type="ORF">AKJ58_01205</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11 12">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11 12">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12 16">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="4 11 12"/>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNH8"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.113"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000026">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11 12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6 11 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0520">NAD</keyword>
    <keyword evidence="8 11 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 15" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11 13">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11 13">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KXB08012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="433" mass="47357" checksum="D10FB579D10C64DD" modified="2016-07-06" version="1">METSPLEIVRLWKRPDRYYLRLLRRSEGEVTGVLPKVRKIVSHVREKGDLALLDYTKKFDGIDLTRDQLCVSKSELEGAYGELESESIKALEKAAETIKRYHRRQLPEEWMEEFESGVYAGEIVRPLDSVGIYAPGGIARYPSSVLMSVIPARVAGVRRIVVCTPPDSEGRIDDTVMAAAVIAEADEVYRVGGAQAIAAMAYGTRTIPKMDKIVGPGGIYVQAAKKVVSSNTNIDFVAGPSEVLVLADSFANPRLIALDLVAQAEHDPSSAAVLATSSKKLAEKVQKEVASLIDETPRRRIVLESIQKYGRIVVARSLKRATEFANDYAPEHLELMVKRPKKVLDQIKNAGAVFIGPYSPIAAGDFAVGPSHVLPTGGASRWSSGLSVMSFLRTPSVQKATKDGLKRLSEVVEKLAELEGLPAHARSVRERLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNI2</accession>
    <name>A0A133VNI2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000019">
        <property type="protein sequence ID" value="KXB07973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNI2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="69" mass="7780" checksum="C394063706996168" modified="2016-07-06" version="1">MGFLYFLKGSSEKKGKKPKSKEGSHKKDKYYCVTCGTEISKGEYEDNEGQCYNCVAEDTVYAAPEEFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNI3</accession>
    <name>A0A133VNI3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000019">
        <property type="protein sequence ID" value="KXB07971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNI3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="90" mass="10603" checksum="B0ABCBA0C2B68401" modified="2016-07-06" version="1">MDAEDLLKAISLESSREILQHLSNGPKTATELREECEELKNRVSFYKSLNRMAKLGLVKKYRDPELKRNVYELKKSRILVNLKRNEVRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNK3</accession>
    <name>A0A133VNK3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNK3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="172" mass="19762" checksum="7FD57CB5A379DA55" modified="2016-07-06" version="1">MLEKEFRSFMDRVIGIENIVGFREDFKERFLRNKKLPSKINKPEKIRNIKRAVEYAKERQGRIELIGFLAGGPLAILSSYIGLTSISIFLGIYWGILFPIELVLRKVTIDILAYKGASERMTGGEILFREAWNKRVLRSAPSLAGIPLVGLLMKLHRKGYEIGMEMLEERMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNK6</accession>
    <name>A0A133VNK6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNK6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="60" mass="6832" checksum="7C264545D009BF2D" modified="2016-07-06" version="1">MNRGLFHEHFSSGAKKKVRLHEAKIGSSNLPGPIIFHFLPLENADEKYGFWRDSMLAASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNL8</accession>
    <name>A0A133VNL8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNL8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
        <property type="entry name" value="THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="320" mass="36932" checksum="6CC809C386B8412F" modified="2016-07-06" version="1">MKVLILAGGYATRLWPITKNKAKPLLPLAGKPILDYILEELEQIQEVDKIYVTTNERFEDSFRGYLGQRGEEKYELIIEKQKAEGEKYGAVGGMMNVIKNKGPSNYLIIGGDNYYSFKIKDFLDFSFEKGDFTNACFRVESLEEAKNYGIVDFDEENKIQEFQEKPESPKSRMASTACYYIPEKRLDVFDKYVNFWKGKIPEDKYLDEPGRFIGWTVERYDVYAYPFEGKWMDVGTREGYLRAEGEIRDSNILKGDIRSSEIGENVTVLENTTVKDSEIENSIIFEDCNIENSIVKNSIVGDKTRIQSKDIREGLIKDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNM4</accession>
    <name>A0A133VNM4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNM4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031857">
        <property type="entry name" value="Integrase_SSV1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16795">
        <property type="entry name" value="Phage_integr_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="77"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="243" mass="27702" checksum="DC291F21E84CDB06" modified="2016-07-06" version="1">MKVAWGLAESTITGRMRCAERLVDFLGGHPAIASKTDILEFLEKYPAQGMYKGIRVIYGRYLKTDLASDVKIPKSGFKPKKIPPDEAIRDTYSNLETLEFKTAYLMLASSGMRRSELMSLTPEDIEFDSRMVTPNKRSATKGAWLTFYNAETEEHLDRLGHLPNQHFPDSYTRAFKEASSGEISPQVLRQWFADKMGRLGVQDRFVDAFCGRTPKSVLANNYSDFRPRRLEKIYKGANLRVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNN0</accession>
    <name>A0A133VNN0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdenum cofactor biosynthesis protein MoaC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AKJ58_01190</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNN0"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.110"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008999">
        <property type="entry name" value="PRK12343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF29">
        <property type="entry name" value="CYCLIC PYRANOPTERIN MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin cofactor biosynthesis C (MoaC)" evidence="4">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KXB08010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="144" mass="15596" checksum="5FCC483E00430220" modified="2016-07-06" version="1">MARMVDVGEKPHVERRAKAEGFIVLKPDTVEKIKTGGIPKGDVLTVAKTAGIMAAKRTPEIVPLTHPIPITGVSINFELEEGGVMAEAEVKSRGQTGVEMEALTAVTVTLLTVWDMVKGFEKDNKGQYPTVKITDVEVVEKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNN2</accession>
    <name>A0A133VNN2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="8">PRA-PH</shortName>
            <ecNumber evidence="8">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisE</name>
        <name evidence="9" type="ORF">AKJ58_01200</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PRA-PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="8"/>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNN2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB08011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="96" mass="11210" checksum="7436A3497EC3359B" modified="2016-07-06" version="1">MDTEILDEVFEVIKDRRENPREQSYVSHLLKEGEKAVFEKVREESEELIEAAEGGERSEIIHEAADAIFHVMVLLAAKGVEFEDVMKELRKRRKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNN3</accession>
    <name>A0A133VNN3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNN3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="84" mass="9735" checksum="9C25A8845719D6E5" modified="2016-07-06" version="1">METGSIVAYLFSKKLESVEIEPGEKIPEKVMANLRKYNLVIPINQYEKLSKGAEKEGTTIQEKVFSKYRYITKIPKEWEVIPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNN6</accession>
    <name>A0A133VNN6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00980</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNN6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="108"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="33478" checksum="722EB70C5C2040AE" modified="2016-07-06" version="1">MFEEFLADYKRREKKKTAETYKKLLSQFEKWLDNQGSQEKFKREDVLSFLKTQHSWSNSSKNVFLSALSSWARSELEKVQPAVTDQEKLEVRRLKRIKGIRSYEARPKEKEALNLDQIVDLRIAMDQDTQNLFWVLLWFGIRVGELKKIKKIGLEDRSLTVETLKRKGSEAERTLYFDDYTGRILQHSRDKGLLDLPYQTIYKRFKKASKLTSIKLTPHVCRHTFATHMSSRTDPFTLAQMLGHSITGVAQARGGAKVTGDYVHPAQERIRKVMLERHYLIPLEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNQ2</accession>
    <name>A0A133VNQ2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent DNA ligase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AKJ58_00905</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNQ2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF7">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA ligase ATP-dependent N-terminal" evidence="5">
        <location>
            <begin position="19"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="4">
        <location>
            <begin position="237"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB08095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="41982" checksum="9A7D1FD26AA0D6CA" modified="2016-07-06" version="1">MFIYSTVILDSKGRIKMRFEILAKTCEELSEKDSRNEKIKITSNFLKGLDRDDLRRACRMIIGRPFPRSSQSSTNLSKKSILNALSRSLKTEKYDEYYAEYGDFGEVIRRLMMEARTKQSVLTGEKVDLESIQAFLDKVSRIGGEGSRKKRDNLADVKFSQISPLEGKYLAKLLLGRSRHGISDGIVVHAIAEAWGRPVERVRRACLLTGDLGEAATMSKMGKLDQAEIEYQRPFLPMLAEMADEAADVVEEMGHCLCEEKLDGVRIQVHRGKNTELYTRNLNRVTEKFLDVVEEFEDFDEEFIAEGELIAERDGSVLPFQRLMRRFREKADENLVKEIPVRIYFFDLLKLGEDSLVDEELRDRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNQ4</accession>
    <name>A0A133VNQ4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AKJ58_01010</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="LHYK01000014">
        <property type="protein sequence ID" value="KXB08057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNQ4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="77"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="137"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB08057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="345" mass="39067" checksum="89DD5157A38D7F4A" modified="2016-07-06" version="1">MKRFEEIFNCINVGIVVHDSEGRIISVNPAPEEIFGLKEERLKEKSLDFWEGKLYKENGEPMNVREFPISRVFDSKKPDKGSIVGVSVSEDEDIRWYLHSAVPQFNEGGDVERVITFSKDITDRKKSEEREAFLETLLRQDMRNKLGVSMGFLELLEEADLPEGYRPYLGKAMDSIGKGFDLVELAEEVRKTRYVEVEEINLCEALENAVEECEGLAKEKGVEIELDSEKRRNVRAGYPLKKIFLHMFRTGIYKPGCSKMRVLDEERDGEVVVRVEDDGDKIPDAVKEKISKGTYTGETSGIGGAIYSAVREIVRNFGGSVEVKDSELGGARFDVHLKKAQQSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNQ5</accession>
    <name>A0A133VNQ5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type II methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N-4 cytosine-specific methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AKJ58_00990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="4"/>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNQ5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0680">Restriction system</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="5">
        <location>
            <begin position="161"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB08076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="366" mass="42277" checksum="9E2683667A1C9D9F" modified="2016-07-06" version="1">MATVSGDWIKKLPLDSQIEIYENIVRKDYTQVELAKIQSELKDEFSKHTEQGRRTDLEDGTSAKNFAEVKRVSDMVGGIFGESGRTVEKRVSVYKQAKENPDDYGDLLERMEESVHSAHRELKRRKDIAEREEKLKDAPELKNLILGDCLEKIEEIPDDSIHLLLTDPPYGTNTVTGARFETEKLREDWTYDGDDESVYPLLNRLFEKMKPKLVKDAHIYIFTSWKAWHRLYPIVDEFFEVKNWLVYLHYMATAGDLYRYRTAASSIMFASNGGKRPIGSHKWNFIDERGTYRKDGMAHHPAQKSISICKKLIENSTVEGETVLDPFCGSGSTLVAAEELGRNWIGIEKERRWYDVAKSRILEARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNR3</accession>
    <name>A0A133VNR3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000015">
        <property type="protein sequence ID" value="KXB08041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNR3"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.80"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="101" mass="11087" checksum="547C63FD19FA1E4E" modified="2016-07-06" version="1" fragment="single">GARGDQRSPANVNFSFRNIEGESTMMHLDMRGISVATGSACATRKLEPSHVLTAIGVDPVVAHGAIRFTLSRMNTEEEIDYTLEVLPKVVKKLRKMSPMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNR8</accession>
    <name>A0A133VNR8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNR8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="79" mass="9154" checksum="51379923F4E41E62" modified="2016-07-06" version="1">MKTKVKKRFSPLERFAIGVIEEKFEKLAEGCEHRFFDNRGKANCRYHPSSVGYCRAEFCPRLTDLQSQDSQKTNKPNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNS5</accession>
    <name>A0A133VNS5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2188 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNS5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018691">
        <property type="entry name" value="DUF2188"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09954">
        <property type="entry name" value="DUF2188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8217" checksum="0AE696D7BF7E212E" modified="2016-07-06" version="1">MMTVDVFHSKDDSAWCVRVRSIGQNRVVSRHRTKKAAIRRAKKEAKALGARIDVYKKDGTLQRTLNYGWQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNS6</accession>
    <name>A0A133VNS6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000014">
        <property type="protein sequence ID" value="KXB08060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNS6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="118" mass="13291" checksum="2ED7840DC75DD655" modified="2016-07-06" version="1">MIEIIYVIFGALIFLVPGYLLSLIIYPGREDLDPWERVGTSIGLGALIVVLTVTLLAQPGIRALQFLPFLAVIVLFCVVCVFLIYQRGDLDRLSFLLEFYRRLSSKKEPVGEEPTSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNS7</accession>
    <name>A0A133VNS7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNS7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF10">
        <property type="entry name" value="SNARE ASSOCIATED GOLGI PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="2">
        <location>
            <begin position="35"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="204" mass="22165" checksum="3A88976C4B4AE1FF" modified="2016-07-06" version="1">MIPKAMTWWQPLVTLGYPGVFLLSFLGASSIIFPIPYTAGLIAFGATGYFDLLLLATIAGLGSAAGEFVGYGAGYAGRKFVSGKHERKFKAMLRIFNKFGAPAIFLFALTPLPDDLLFIPLGLARYNFLKAFIPCALGKFLMCLILVHTGSVAGQMFAAGWLLALVTAVFLVLIIYLVLWVDWVKIADRFAPEPKDSAKFIGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNU3</accession>
    <name>A0A133VNU3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNU3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF7">
        <property type="entry name" value="CARBON-MONOXIDE DEHYDROGENASE ACCESSORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="2">
        <location>
            <begin position="84"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="332" mass="37392" checksum="BC26207FAE7B8A13" modified="2016-07-06" version="1">MPMGEEEPMELEIKKESPSSKEERERVEEIVEKNLNRVIEILRAKDYGIIEPVKSIRQEVKEGLEEAPEVGERRGLETRVIGFAGKGGVGKTTLAALFIRAIGEEEGSPSILAMDSDPNTCLPEMLGSQDYETLGDMVEKYRGATLPIQKFRQEFASLLLRNEQEGYDLLPMGRSEDQGCYCMVNNLLRSSFRDEVLKGSYAYDYVVMDCEAGIEHISRKTSAIVSDLVIITDGSQLGMNTVRNIREVGRKVKIDIDNFYVVVNGVEDEEIIKDVKNLSDELKITFLGTVPYDDAVKKLSFRGGSVFELSEDSRAYSKVKEFVRTMLSRDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNU5</accession>
    <name>A0A133VNU5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNU5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="119" mass="13864" checksum="9B68F18908CAE443" modified="2016-07-06" version="1">MSTEIIFEIIMIIVVGIAVYYGFKSGYKGQEKQDNRIKQELEKSEKITNQQYFILRLPLSILLGWSILSFVFSLNGLTIILPYMVNSTMAWNWAIFLIVNFLVGMLVWMGIEKVYLWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNV1</accession>
    <name>A0A133VNV1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNV1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="107" mass="11174" checksum="3FE57ACAD0CBDF46" modified="2016-07-06" version="1">MVDELSRGEVKFTVDSIPEADMSNVPGGYRKTGPSYVWTGDGAADILATLLTGKGGYSIVIPPSGGTGEGVPEDKFLYGIVIAMAAVLVILIGVLFWKGRGGRSQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNW5</accession>
    <name>A0A133VNW5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="9">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="9">Ornithine acetyltransferase</fullName>
                <shortName evidence="9">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="9">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="9">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="9">N-acetylglutamate synthase</fullName>
                <shortName evidence="9">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="9">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="9">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="9" type="primary">argJ</name>
        <name evidence="10" type="ORF">AKJ58_00820</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ArgJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="9"/>
    <dbReference type="EC" id="2.3.1.1" evidence="9"/>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNW5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.402"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity, acting on acetyl-L-ornithine as donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.340:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.70.12:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="entry name" value="ArgJ beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003802">
        <property type="entry name" value="PRK05388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100:SF0">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023569526" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023569527" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="9">
        <location>
            <begin position="192"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="9">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="9">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="9">
        <location>
            <begin position="191"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KXB08139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="409" mass="43292" checksum="B485EF8CA3B95028" modified="2016-07-06" version="1">MVSIIEGGLTSVPGFRATGVEAGIKHGGLDLLLLSSDKGPIPAAALFTSNLVKGSPLQITKEHLQNGKLGAVVANSGCANAYTGQQGIQDARETAELVAELLDINPGDVGVASTGLIGTRLPMNQIREGIKKAVKQLSNSWEDGTKAAKAIMTTDTCPKEIAIELELEDGTRTTIGGATKGAGMIHPDLHATMLAFIVTDASMTPAGLRSALQRSADNSFNMVSIDRDTSTNDMALAMANGLAKNDRITKKHPSKKFQKGLDFVTTELARMIARDGEGATHLLEVHVEGAESRKDARIAARAVVGSNLVKAAVFGEDPNWGRIVAALGYSGASFDPSKLSISFKSEEGESLILFRGKTTSEENLIRAEEVMGEEKIEIHIDLGAGQESSTAWGCDLSKEYVEINSKYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNX1</accession>
    <name>A0A133VNX1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNX1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="131" mass="14714" checksum="A9FACBB8DF704B53" modified="2016-07-06" version="1">MKVYDEHIPEELYGKFLSRLPQVSVELFLLNDWEGKLISEIEWARKPLDLIPPAFSEQLLGLKIEKINCTELTWAEPNLEIGLKLTLTGDLVNNEESVGYPGVALEGSFEVSEASWSQTVDASFQITEEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNX5</accession>
    <name>A0A133VNX5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="5">OTCase</shortName>
            <ecNumber evidence="2 5 6">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AKJ58_00805</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2 5 6"/>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNX5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.399"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000008">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="9">
        <location>
            <begin position="5"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="8">
        <location>
            <begin position="151"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="231"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="266"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR00658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KXB08138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="306" mass="34468" checksum="F0B45AD02CE70171" modified="2016-07-06" version="1">MAKVKHLLSVLDLSDKELLAILKRAEEFKSNLGKKKRYEPLYGKSLAMLFTKPSTRTRISFETAMTQLGGHAIYLDQHRSQLERRETIEDTGKTISRYVDALVARFHEHEKIEKLARNSRVPVINGLTDLLHPCQTISDLFTIREKKGRLEGLKLAYVGDGNNVCNSLLLSCSKAGVNISVANPKGYEPDATIVKNAKEKAEEKGTKLGIITDPNEAVLETDVIYTDAIVSMGQKGRERRLKDFREYQVDAELMKKAKKDVIFMHCLPAHRGEEVSAEIIDGPHSVVFDQAENRLHVQKAILEEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNX6</accession>
    <name>A0A133VNX6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_00865</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNX6"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.32"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02034">
        <property type="entry name" value="CooC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001573">
        <property type="entry name" value="Carbon monoxide dehydrogenase accessory protein CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF6">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="261" mass="27981" checksum="294B23E4C1AF5C32" modified="2016-07-06" version="1">MINIAITGKGGVGKTTIAGTLARLLARNGYEVLAVDADPDMNLDSALGTDASDITPLSKRKKLIEERTGMKPGGISIPGVFKLNPKVDDIAETYGIKAPDGVNLVVMGTVDEGGAGCMCPAGAFLKALMRYLIKRDKDAVILDMEAGVEHLGRGTAEGVDAMIVVVEPGLRSVETAERIKKLAKDVGIKDLKGIVNKARNEKNCGPICSRLEKLGIPVLGTVPYDESFTEADLERKAPLDYNQNSRGIREIRRIEKKLIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNY6</accession>
    <name>A0A133VNY6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1 2">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name evidence="6" type="ORF">AKJ58_00830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1 2"/>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNY6"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.404"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="5">
        <location>
            <begin position="362"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR00838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB08140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="491" mass="55219" checksum="EC38897714714870" modified="2016-07-06" version="1">MLRRGLFKKEMDPDASDYTSSMEEDIRIFKAVVQINAAHVQMLEKCEIIEKSEKNKIIRALSELYEKGVEALDLRPELEDIHMAVEEYVNKKAGKKVGGKLHTAKSRNDQVSTAIRMALREEVLELQEALIELINELVVLASKHKETIMPGYTHLQIAQPTTLAHYLNSYSQAFSRDLKRLNEDYGTINYCPLGACALAGTSFSIDRKLTSELLGFDEIVENTIDAVGSRDFALQTMSSLAIMMSNLSRLAQEIILWSSTEFDTIEVPDELASTSSIMPQKKNPVVAEIARAKASRSIGELISGLNIVKSLPQSYSLDLQELTPSLWSAVDQSKTSLKVMKKFVRCIEAKPERMRKLAKKGFSTATELADALAKWTEVPFRESHEIVGRMISQALENGKELEDLSLEDLQAASQDAIGKRVELSEKRFRESLDLKSCIESKGKLGEPAPESVKKELSKLEELTKKYKKILEKRKKILSEKSARLFESKENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNY9</accession>
    <name>A0A133VNY9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formylmethanofuran dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNY9"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.37"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02761">
        <property type="entry name" value="MopB_FmdB-FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016457">
        <property type="entry name" value="Formylmethanofuran_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03129">
        <property type="entry name" value="one_C_dehyd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF14">
        <property type="entry name" value="FORMATE DEHYDROGENASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005646">
        <property type="entry name" value="FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin oxidoreductase" evidence="2">
        <location>
            <begin position="51"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="431" mass="47159" checksum="14AD23A34CA0D5C8" modified="2016-07-06" version="1">MSETITDVVCSFCGCLCDDIGVIVEDGCVKDTINACRISHAKFTEFNNGSRIEEPMIKADGELKPAPLEEAIEKSADILKGSKYPLAYGWSSTACEAQKIGIAIAEELGGIVDNTTTVCHGPSVLAEQGVGYSGCTLGEVKNRADLIIYWGCNPMHAHPRHLGRYTMYPRGFFRDSGERDRNLVVVDIRKTDTAKLADHFIQVEPNQDYELLASIRVLLNGGEIPEEVAGVSSGDIEKLAEMMKNCNYGALFFGLGLTMSKGKYMNIDNAISLVEDLNDYTKFVIMPMRGHYNVAGANTVSNWETGFPYSVDFSRGYPRYAPGETSAADVLMNGEVDSALIVASDPASHMPRKALEHLRNIPVISVEPHMSPTAEISDVVIPSAVVGIEAEGTAYRMDHVPIRLRKILDPPSGILSDEEILEMLFRKIRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNZ0</accession>
    <name>A0A133VNZ0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetylornithine aminotransferase</fullName>
            <shortName evidence="5">ACOAT</shortName>
            <ecNumber evidence="5">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">argD</name>
        <name evidence="7" type="ORF">AKJ58_00810</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="5">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="5"/>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNZ0"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.400"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002325">
        <property type="entry name" value="PRK01278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 5 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="107"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="220"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="396" mass="43545" checksum="40F4321D3CBC7E92" modified="2016-07-06" version="1">MRLESILDLDKKYVAQTYERQPIVLSEGSGIKVKDIEGNEYLDFVAGIATCALGHSHPAVTETISKQAKKLVHVSNLFYNRPQVELAKALAGVTPDSIKKFFFCNSGAEAVEAAFKLAVKHAGRSHIIALEGSFHGRTLATLGATWKKSYREPFKALIPPAFKFVPFDDLDGIKSVINDRTAAVIAEPIQGEGGINVPSENFLSGLRDICDDFKTLLIFDEIQSGMGRTGKWFASEHWNVEPDIMTMAKALGNGFPIGCMGAKSEVMDSFSPGDHASTFGGNPLACTAGKAVVETMKKERLPQHAEEIGRYFKKKLKELAKKHKSVQEVRGRGLMLALELVDEKSARLSVTKAREEGILINRTAKNVLRFVPPLIIEQKHVNQLIKTLDQILKVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNZ2</accession>
    <name>A0A133VNZ2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNZ2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="69" mass="8114" checksum="D606F2EE9E2B7E66" modified="2016-07-06" version="1">MLAILLQLFLLSLLLFLFFLLLDDAYLSRFRIVFLNLLPFIDSKGLQIFPSFLFILKFVYVNPPFLKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNZ5</accession>
    <name>A0A133VNZ5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNZ5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029349">
        <property type="entry name" value="DUF4443"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054039">
        <property type="entry name" value="PH0730-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14544">
        <property type="entry name" value="DUF4443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22167">
        <property type="entry name" value="PH0730-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH0730-like N-terminal" evidence="6">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4443" evidence="5">
        <location>
            <begin position="91"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="188" mass="20630" checksum="F4D31D161D549BA9" modified="2016-07-06" version="1">MPASGPQPRFSEIHEYMALELIREYKSLGRKQLSDKLGVGEGSGRTILKRLKEKKLVTSTPQGHVLTEKGRRKLKRREERFVQLPASDMTVGNVDIATIVRGGAKKVRDGIKQRDEAIKAGAEGATILVMKESELQLADRSAEVNKKVAAKIKNSFHPQEGDAIVIGTGKNREEAERGTLVATKSLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP06</accession>
    <name>A0A133VP06_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Formylmethanofuran--tetrahydromethanopterin formyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AKJ58_00675</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FTR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP06"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.371"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030270">
        <property type="term" value="F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.520">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014053">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002770">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023447">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_fd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022667">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002554">
        <property type="entry name" value="PRK02114.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01913">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02741">
        <property type="entry name" value="FTR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006414">
        <property type="entry name" value="Ftr_formyl_trnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55112">
        <property type="entry name" value="Formylmethanofuran:tetrahydromethanopterin formyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formylmethanofuran: tetrahydromethanopterin formyltransferase Ftr N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Formylmethanofuran: tetrahydromethanopterin formyltransferase Ftr C-terminal" evidence="4">
        <location>
            <begin position="153"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KXB08179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="300" mass="33100" checksum="2FEB8BEA13E8B8BD" modified="2016-07-06" version="1">MSEKAEIEDTYAEAFKGIYCRVIVTAAYKETVKKVAEDATATPATVIGRTEAGIEKWLEKEETPDGRPGAILQFWGGIQKGLREAIEKFEKELSYRIRQDILVKPFTSVFDANPAPKGSLDMMKRVGHCGDGYEWMEERFGREMIVVPLMVPNFQIERRLGYATGVMGGNFWYLCKTKKAVMKAGKKALKAIEKVEGAVNTFDICSAGSKPDTNFPEIGPTTNHPYCPSLKKQLGSKSKVPEGVKYIPEIVLNAISVDAMKNAMKAGIQSARSVEGVVKISAGNYGGNLGEYKIHLNELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP17</accession>
    <name>A0A133VP17_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IMP biosynthesis enzyme PurP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP17"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.379"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006188">
        <property type="term" value="P:IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP biosynthesis enzyme PurP C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="109" mass="12544" checksum="72B8B4F5D86B7287" modified="2016-07-06" version="1">MVYENAEKLMKTLKEEVPPGMIGPIGIQGAVPIDEKDRPEFVIFDLSFRVPGDPAIGPTSPYLRYLDVKHEEEYAKFMPSNWKIKEPLDLSMMEIKRAIHEQKLEKIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP20</accession>
    <name>A0A133VP20_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="7">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxT</name>
        <name evidence="10" type="ORF">AKJ58_00680</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EC" id="3.5.1.2" evidence="7"/>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP20"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.372"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000041">
        <property type="entry name" value="Glutamine amidotransferase subunit pdxT, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="7 8">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="144"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KXB08180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="199" mass="22037" checksum="6E5E1D2FACF61ACD" modified="2016-07-06" version="1">MKIGVIGLQGAISEHILAVELAMEALGVEGEAFWVRKGEDINDLDGLIIPGGESTTIGKLMVESGLFQKVQDRADSGMPVLGTCAGLILLAKEGDEQVRRTGQPLLGLMDMQVIRNAFGRQRESFEVDLKIPVFGENAFRAVFIRAPAVKRVWSDVEILAEYQDRIVATRQENLVAMAFHPELTPDTRAHEYFLKICRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP27</accession>
    <name>A0A133VP27_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP27"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="59" mass="7457" checksum="DBD85A5EF8F434C4" modified="2016-07-06" version="1">MIEIHERIKEKLNEEGIDRTIKEFLRKILAEELRHFEEKRWRYGKKYDQYIKKYASVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP31</accession>
    <name>A0A133VP31_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP31"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="14"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="60" mass="7013" checksum="054159BCC5B505BD" modified="2016-07-06" version="1">MPKIVRESSVTQARKGTTSFRTTIPREIAEQLELEHKDKITWEVEPGKEEIKAIVHRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP34</accession>
    <name>A0A133VP34_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="7">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purC</name>
        <name evidence="9" type="ORF">AKJ58_00700</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="7"/>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP34"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.376"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000015">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010568">
        <property type="entry name" value="PRK13961.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="8">
        <location>
            <begin position="14"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB08183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="278" mass="31980" checksum="5A863C006B70FC81" modified="2016-07-06" version="1">MEALIESNLPLKRFKRGKVRDIYDLDDKLLIVSTDRISAFDSVLPNGIPRKGEALNRLSAFWFNQTEKIIPNHMIDVIDQRSMLVRKAEPIKIEFIVRGYLYGSAFRKYKQGKKISGIELPEGLKKAEKLPSSILTPTTKAEQGHDVEINRKQVARKIGNELTDEIEEACFKIYEKASRKALKNGILIADTKFEFGLCEGDLIQIDETLTPDSSRFWPLGKYELGKDQYSFDKQYLRDYLDNIGWNKEPPAPNLPDEVIAQTSKRYKECYERLTGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP37</accession>
    <name>A0A133VP37_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP37"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="170" mass="19385" checksum="284BB783659B2183" modified="2016-07-06" version="1">MIDNKWFCEPNDERESWCFLILEQRNSEGDGSITGWQIGYGNPKKMTRTLSSSGSSEEEIIQGLLDEIYYCRREGILMITAERDSVPALRTHILLLGMEEASFRGVEYVCIDRLLEEHFLGLGDLDGSSGRSILELGERIGIVEDCEETELLRRIFLRIGPLLPKSFPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP39</accession>
    <name>A0A133VP39_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Elp3/MiaA/NifB-like radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00625</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP39"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409">
        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409:SF15">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="6">
        <location>
            <begin position="122"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="358" mass="41141" checksum="E21FD946D798B35C" modified="2016-07-06" version="1">MKILLVEPEYYSKYPPLGLLKLSALHKEKGDKVEFVRGVDIGISGKFDRVYVTSLWTWGWRKVWECVRYYKRLTNAEVWLGGIYASVLPDHAKKSGADKVVQGLYPKLRKVVPDYSIVPEWDGSILFSTRGCRFNCPFCAVPKIEGDLHVVKNPIEPYIHPNHTRIILWDNDFLGSPRRDQVIEELKEIGKKVDFNQGLKASQIDNKIAGKLTELNLNGSDGSVKIRLGYDREEEGPAVKRAIENLKDVGIKGRRIMTYNLFNFDENPNEFLERVTNTLNLGVVSYPMRYQPLKGEYVLEKDSYVSPEWTREELEMVARARRVIGYGGSFPPYKGLVNKLNECESFWDCFKEFSERGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP51</accession>
    <name>A0A133VP51_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CobW/HypB/UreG nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000006">
        <property type="protein sequence ID" value="KXB08229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP51"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF62">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="1">
        <location>
            <begin position="24"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="363" mass="40172" checksum="A05754A422D6EDBA" modified="2016-07-06" version="1">MSDRIRGKFKMRDQDKRKLERMKVATIGGFYAVGKTTIITKMGKKLSENGKRIAVVSNDKGAISVDGEVIKKLGFETGEIAGGCVCFYLPLLKHIIDTITQKFVPDVLFLEPVAYFLPSKLYSDLRKKFDGVELAPVIILADASRLLDYKERSEELSYLESRQVKDAEVLVINKVDLVSESQLSAVRKMVQRINPKARLVETSGKMGAGLGELSNITLQERHEGAPPVSKAVVKDFAKSVTEMGERGEQYAIGTKKDLSESDVKNFLRDLLRGIAEKVFSAGGEINHIKALLGNESFFLKASLVRLDQEVDFVGKIKGLSSGSSGRIAINAFGRGLPDDFLYNLIITTLQEVAPKYEIKFDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP53</accession>
    <name>A0A133VP53_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000006">
        <property type="protein sequence ID" value="KXB08226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP53"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="93" mass="10373" checksum="78E8F1437D0C5051" modified="2016-07-06" version="1">MEVTIRICECLHEEAGTDLIKVPIGDRETVDTLLIKLGRRSPSLIETVLDPRTGGLKEDFDILLNGRSIQSIRGIHTKLKNKDELTISSSETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP64</accession>
    <name>A0A133VP64_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cold-shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AKJ58_00425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP64"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050181">
        <property type="entry name" value="Cold_shock_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11544">
        <property type="entry name" value="COLD SHOCK DOMAIN CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KXB08249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="64" mass="7065" checksum="C15240E19CCA4365" modified="2016-07-06" version="1">MKGTVKFFDSRKNFGFIAPDEGTDDLFVHASDIESGTLNDGDKVEFDSEQGEKGPRAVNVKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP75</accession>
    <name>A0A133VP75_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribbon-helix-helix protein CopG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP75"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8566" checksum="507885C28003CB2C" modified="2016-07-06" version="1">MPTVSVRVSEDLKATMEKHKEVNWSEVIRQAVKEHAIRIELADEITSKSKLTEEEARQIGERIKEGIAKEHGLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP82</accession>
    <name>A0A133VP82_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP82"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="122" mass="14077" checksum="CB602C69E7ACB2D9" modified="2016-07-06" version="1">MVDYLVVIPWLHEEPELVLAKGENRRAVYDYYDHNLAATPIVIPLKRGIDLGTPNDMEFKCKNCSNPIPAEDEDPRALEDAVRIQQGGAEHPCHYSVWWFCDMKCFSEYVVKQLVKGINNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP88</accession>
    <name>A0A133VP88_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP88"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="180" mass="20303" checksum="9F131311889D520E" modified="2016-07-06" version="1" fragment="single">REGGADICGIYKIPSKFRKDLFGYSPFCIRVVLQAKKESIDSPDVELFSKQVDNLQRACQTGDMSISRIAQRLPNSFTKFSFPQVPIVPLMFTAGRPSGLDLFTNISEAEGAVETARNSAIMWRNIDQLATDFINLAGMWFDETNCFKRELFLEQFSKMSGYNGSRENVEKTIFTSPPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPA2</accession>
    <name>A0A133VPA2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Addiction module toxin RelE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPA2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="11448" checksum="4E0F9EB08F669705" modified="2016-07-06" version="1">MKNSFRLTRTDKFDERFEDLTSETRERVLTKLKEFRHQVNEYDVDPRQHGSTKYIAQKRVWRLRIGDYRAFFDILGDEIRFLTILSRKEAYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPA7</accession>
    <name>A0A133VPA7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPA7"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.308"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="223" mass="25663" checksum="0FFD30844D4B4683" modified="2016-07-06" version="1">MKRTTGLKKFCDRCLNTERYEGNVVLMVVDAAFDSIGLNYFNSIVPKVLEFEEKFVKEGDIQNLEELSNRSIKQVKSIWANERSWNVAFSVASYLHELGESRGLNDRQALRTWAQNADLENWKENPIGQIDGVGLTTYQYLRMMGGVDTAMPDKIVRRVIEEILKEAEGDMPTENDIEFVKTVDRIAFSTGYRAIEICWMTWLVQSEGGKVRMEKYGDVLERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPA8</accession>
    <name>A0A133VPA8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dam-replacing protein HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPA8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22319">
        <property type="entry name" value="DpnI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.210.30">
        <property type="entry name" value="Dam replacing family, catalytic PD-(D/E)XK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010324">
        <property type="entry name" value="DRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041368">
        <property type="entry name" value="DRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043025">
        <property type="entry name" value="DRP_PD-(D/E)XK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06044">
        <property type="entry name" value="DpnI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17726">
        <property type="entry name" value="DpnI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dam-replacing protein HTH" evidence="1">
        <location>
            <begin position="185"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="262" mass="30373" checksum="CCB04D70899A7470" modified="2016-07-06" version="1">MNLELDPSVAEEYKNPAQITRVVSEAWADENLYCPACLNDSLSKARTGRPVLDFMCPECSEKYQLKSKKSPFGNKVSNSAYRPKIEAILNGTIPNFAFLQYDSSDWVVRELFVVPSHFMTESIIEKRKPLPASARRAGWVGSNILLGNLARDAKIPIVRDRKALHRKLVKRWWDQFSFMREQSVESRGWLSDILMCVRRIDQEAFTLDQVYEFEEELSKLHPKNKHIRPKIRQQLQVLRDKGVINFLGDGWYLVKDLEALDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPB2</accession>
    <name>A0A133VPB2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AKJ58_00415</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPB2"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.316"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06164">
        <property type="entry name" value="S2P-M50_SpoIVFB_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016483">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39188">
        <property type="entry name" value="MEMBRANE-ASSOCIATED ZINC METALLOPROTEASE M50B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39188:SF3">
        <property type="entry name" value="STAGE IV SPORULATION PROTEIN FB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006404">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 14" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 14 16" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="18">
        <location>
            <begin position="246"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="18">
        <location>
            <begin position="308"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR006404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KXB08267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="371" mass="40823" checksum="924E400797572D6B" modified="2016-07-06" version="1">MKTSFKIGEISGIPVRLHITLLFVVGFIAWSIGSNIFQIAEIIGVDPSGISPGIESYLIGAAMAIGLFVSIFLHEMAHSLVARGMGVNIEEITLWIFGGVSNMEEIPHEPDLETKISIVGPFTSLGIGVGCYLAGIISPINSLAFVFTYLVILNVFLAAFNLIPAFPMDGGRILRATFARGESYISATEKAASIGKAFAFVMGVFGIFYNPFLILIALFVFIGASQEAQGVMVRDVLNKVKVKDIMSEDVRTVSPNMGLREFLDRVLEVQHTGFPVVENEEIVGIITMEDVKKVEQDRVDEFTVGDVMERDVFCFTPESNVNDVWQMMAKKDVGRFPVVSDRKLVGIITRSDIMRSFKALSEIERYRGGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPB5</accession>
    <name>A0A133VPB5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SbsA Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_00380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPB5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SbsA Ig-like" evidence="3">
        <location>
            <begin position="325"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="432" mass="48918" checksum="EC6E28E58FA26246" modified="2016-07-06" version="1">MIYHYSLVAYLCHLIEEMGGKENGEGLWDTPEEYKDNKYLVFSSLTYSQTPIAMVTESASISFLNSEDGSKNNSDGKNQALIISGAPGNDPNGDISNTWSSWWIMMRDNFGFDVTMPWKPSENTVDNTMENLSKDLNPCENFSILILGHGREADGAVMLDDGSFLTPEKFSEFICRIENGVHISVTVDACYSGHWIEHLKKWDKKVEIVYTSTDNDSVGWGDIDNIDNTVTDSNPEDSGREATSGLMEDLKALADNFKRGDISWPALWQEAIKTANEKNMIVQNDEFFKEWTENAYPDLYPACSGKGVQNPQQWKSEKVRDHAAPYVKQTNMSTMLFDTKDNIMVEFSEAMNKTASQKAFSITPSTLGEFKWIENTLIFSPSEPLEFETQYEVRVNTQAMDLAWNRLEDNYSWTLTINGSLEGDFSYQPLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPB8</accession>
    <name>A0A133VPB8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glyceraldehyde-3-phosphate dehydrogenase (NAD(P)(+)) (phosphorylating)</fullName>
            <ecNumber evidence="2">1.2.1.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AKJ58_00545</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="2"/>
    <dbReference type="EMBL" id="LHYK01000006">
        <property type="protein sequence ID" value="KXB08231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPB8"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.347"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043891">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase [NAD(P)+] (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000002">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="12">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="10">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KXB08231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="336" mass="36503" checksum="CC91E7A07C6B6493" modified="2016-07-06" version="1">MKLAINGFGRIGRCFLRAALERGENFDCVAVNDLAPASTLAYLLKYDSTFGILKEEVNATEDSITVEDMEMKFLSERNPADLPWEDLGIDLVIEGTGIFREREKAAKHLDAGAKKVLITAPGKGEGADITLIPGVNNDQYDPEKHKIMSLGSCTTNCLVPTMKVLLDEFGVEKALMTTTHAYTNDQRLLDAVHKKPQRARSAAESIIPTTTGAAIATTKVLPELEGKMHGIALRVPTKDASVVDLVALLGTEVTEDEVNSAFKKASKGDLKGILDYTEEPLVSVDYIGNPHSATIDGPFTKVRGNNLVKVLAWYDNEWGYSCRLVDMANQIGAGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPC1</accession>
    <name>A0A133VPC1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DM13 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPC1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019545">
        <property type="entry name" value="DM13_domain"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51549">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM13" evidence="1">
        <location>
            <begin position="20"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="130" mass="15085" checksum="2F0DF61AB4202F08" modified="2016-07-06" version="1">MGRGTDSIYTKPDNPTTTPGYWTELTANNEITVSGTATYEYPEKIVIENFTFQGDDREIEIRLADNRVEEAGLLVNVARLINIEHKNYDNAELVLHWPDHLTERDFNHLIIYDVDNREVLGKYKFLPLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPC8</accession>
    <name>A0A133VPC8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPC8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="127" mass="14689" checksum="A439F1C76A8D70F2" modified="2016-07-06" version="1">MESEEIEETDKLIRELLGLSSDLKIFRDFEDQIEEGLRNFQRENYHASFNTLLDTVEGVVRKIFTEFGFGGTSGALVPMIEKLRAEKLVRKGTEHLIRGLDRNLPNHGLLMIIETFQKPSLNFLFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPD5</accession>
    <name>A0A133VPD5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPD5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13332" checksum="0FEAB2C90AA03E51" modified="2016-07-06" version="1">MSGFVSRRKPSARPRRISDIKTGDEQIQVVGLVVDKGEAELFLDDGTGRLPVLFEDPGTVDDIDIGSKIRVFGTPLSVADSYELHAEIIQKLEGLDLNLYRKVKHEEKKFEKELDDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPE4</accession>
    <name>A0A133VPE4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide 5'-hydroxyl-kinase</fullName>
            <ecNumber evidence="2">2.7.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_00260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15669"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13184"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-phospho-ribonucleoside-RNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54580"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.78" evidence="2"/>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPE4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051734">
        <property type="term" value="F:ATP-dependent polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 P-loop" evidence="10">
        <location>
            <begin position="24"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB08324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="329" mass="36749" checksum="7CE9A3DB930F9291" modified="2016-07-06" version="1">MEVPVHWRKAIDKVNQAKISLVIGETDSGKSTLVSFLHNKLGGSIIDSDIGQGDIGPPTTIALSTDGEEMLDGYFVGSITPSRHFCEMLIGTKRMVERASTPIFIDTTGMVHGSAARALKTGKIESIEPDLLILLGDGELDYYYTYKRVGIDVIGLPKSKNAKKRSRAERRKARKKAFQEYFADSEQRRIDLERLGLERTIVGSGENISEEISELVDVKVYRAERLGDTILLISDRSRIRPTTYKNVKRRMGVEKFNILKASDFENLLVGLVGRKNDFLGIGIMEDLDFDSFQATIFLPRNTVKRVASIQLGSIRVDRYGEELGLISLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPE8</accession>
    <name>A0A133VPE8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPE8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="99" mass="11616" checksum="989463BAC79C67BD" modified="2016-07-06" version="1">MTDLEDIPKEKLTKTAIHLIFMSVDNCEKFVDLLGRKPEMPLEEVRDNVFGEVYGKHDVGRVSHKTWEALGEACMDRQMYYQDLVENVLKKMVKRKRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPE9</accession>
    <name>A0A133VPE9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nop10</name>
        <name evidence="8" type="ORF">AKJ58_00235</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPE9"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.244"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009623">
        <property type="entry name" value="PRK13130.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB08320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="57" mass="6435" checksum="B06ED2E35CA2D0D8" modified="2016-07-06" version="1">MKKCKGCGSYTLKENECPKCGGELGTPHPPKFSPEDPYGKYRRKLKKEALDFGKEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPF3</accession>
    <name>A0A133VPF3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPF3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="68" mass="7906" checksum="871897AB53A0548D" modified="2016-07-06" version="1">MKEIKIGLSEERVKDLVELVKASIKLSPRQRFEILREAQERVMRVHGCCSLAEVERLSEKAEKIAREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPF6</accession>
    <name>A0A133VPF6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPF6"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.246"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19067">
        <property type="entry name" value="PfuEndoQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005287">
        <property type="entry name" value="CHP00375"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00375">
        <property type="entry name" value="TIGR00375 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40084">
        <property type="entry name" value="PHOSPHOHYDROLASE, PHP FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40084:SF1">
        <property type="entry name" value="PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="43666" checksum="BD0255251AD42CE1" modified="2016-07-06" version="1">MILDIIAQQASFKGLTFVGTGDFLHPSWMKQVQAKLEIVEEGTFEHPRFGTRFILTVEVEDDRRVHHLIISPSISSAEGLYEEFSKHSKDINLDGRPSLNLSAPEIVEISRDHDCLVGPSHAFTPWTSIYKEFDSISSCYEDQVDKLAFLELGLSADTLMADRLMELESVPFLSNSDAHSPWPNKLGREFNRFELSRPVSSEMIESIREKKGISLNVGLDPKLGKYHMTACSKCYSQFLAKDAEKYGWRCKSCGGVVKKGVLDRVSELADFESPLHPDFRPDYLRIAPLSEVIALALGCSNPRSRKVRRTWNRLIEHFKDEITVLVDADLAEIEETSGPQVALIIGLFRKRQLDIDPGGGGRYGRLKIPEELIKAQRPGGQRTIAEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPG2</accession>
    <name>A0A133VPG2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="11">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">priS</name>
        <name evidence="14" type="ORF">AKJ58_00215</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="11"/>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPG2"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.239"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.160">
        <property type="entry name" value="DNA primase S, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 11 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 11 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KXB08317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="316" mass="36765" checksum="821781236E9FBF8C" modified="2016-07-06" version="1">MQEMREATPEERRQYYSEEWKKDDLPKFFLHTLSLREFAFDHSGKGPNDRYNCFITPEELSDFLRKKAPYAVYGSVALYERPSQRRKWLKSELVFDIDAKDLPIKRCDCTGGNVCEKCLDDARQVAKIIAETLRSELALHDIHFVYSGRGFHIRVTDDSVMSMGQAERSQIVEYVAGIVVPSDMTLTLGYSRVFRKRVARIFEQLDEKKLVDAGFRGRLARKLMREKEKALDAIRRGHLEEIMEFDGIGRKTFRKFLNVVTRLNSELVDGKVTIDTKRILRLPSSLHSTVSRKCMMIRDIDNFSYDATVPRFLLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPG5</accession>
    <name>A0A133VPG5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AKJ58_00290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPG5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="5">
        <location>
            <begin position="139"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB08330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="509" mass="57257" checksum="FE0CF1E86665E3C7" modified="2016-07-06" version="1">MFSEIGTVICTLDGPSSTEFSFVVTNCAEDIPVKRGQFVELESEEGKMVAQVQDLIKTNRYFMRAESVREYERGNKSIASVFPADRWEYIVARAKASGIQTEDGIKQVSFPASPGQKVFTAGSDTLASFLGINAGDGVDLGKLRYHDLSTKLNLTKLLRKHVAILAMSGAGKSYSAMVLLEELMDRAENEGRIASIIADVHGEYTSLARPSEEVDYSDRAFVVEGKNIQIGAPDMSAYQFREFAPDMSGSQTRELNRIIRQLQSEMRSGKGRYDLDDIIREVEKDGETKKQIRKALADRLYDLKNLHIFGKNDYPNWRELAQPGKVAILNLSDILNLRKKRIILAYFARKLFNKRKSGQAPPFALFLEEAHQFAPSKRSVISKPVLRTLAREGRKFYASLILISQRPVKLSTTVLSQANTHIILRVTNPYDLDHIKKSSERITGDTIDIISSLPVGEALVVGEAVNHPVFVKIRKRRSQEAEHGTSLKEVARHFEEQRIQKREDAKAFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPG8</accession>
    <name>A0A133VPG8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPG8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="474" mass="54592" checksum="A21F0FCF13182D20" modified="2016-07-06" version="1">MDLKLPTTFSVVFLALLLLFPILVNAQTPAIPPNMTAEEFEKIVKRANKGEITEQFPQYRRRWAEEEPGWRTCMWYFQGDTSCNDPMFDEPCEVCYSKDYQSNCYLYPAPAGKESDKCQGLNPSDCCEVTKDYYLKHDIWGAREMYYRCYCKIPTPTCPDGHEPGYKKCMDVAWTTINGKEVVAEGVFECSDQGTYEYRGICSEGGCENGRGCFDICPTCDQNQVCKFTDTIGVSYCKDCHPRTQKDCYNGNAYWFDSCGRKESVAEYCSGYEECVSGECRITAKPKRPERPGIWGVLSDVWSDFVNWLRSLFKFSQTTLHQEHVAKEYRRGTRVNASVVLDTSGLSKPDTDHTDGDLYYLSKCYARLPVGLKDLDRAVDRNCVDLTSEMNGKMTYSFTLDKESDVYFFLTEQKGTYSWETGTWSWDTDILRKRSKIMEYRIKEAKPPAKRPGIWDTISKIWNDFINWIKGLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPG9</accession>
    <name>A0A133VPG9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="4">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pcn</name>
        <name evidence="9" type="ORF">AKJ58_00205</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPG9"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="8">
        <location>
            <begin position="122"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB08332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="246" mass="27406" checksum="85C707D00D88F418" modified="2016-07-06" version="1">MGNSELWKNCIGPISKLIEEAAFTFTPEGVRMKAMDPSRVALVNFELSADGFDSYDIEGKRILGMNLEEMSKFFSRAKKSDELIFSFQEGENRLVLTFKGTSIRKFTLPLIEIEEKELPEPELEFTASAKVLAGMIKDGLKDASLVTDKVRFELLNEYFLMTTESDTGSAEMKLPVGSEGLQELKVDEAARAMYNIGYLDDIINVASSNDTIEIHLGTDLPLQLDFSLAGGKGRLRFLLAPRIEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPH2</accession>
    <name>A0A133VPH2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sialidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPH2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1207"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1230" mass="135676" checksum="F5E9E4ADA0C3DA7A" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPI1</accession>
    <name>A0A133VPI1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPI1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="93" mass="11273" checksum="24A5561F47E3B159" modified="2016-07-06" version="1">MSEDEKVRCPICTRRVDSKNEEWWFRYTDKEFKEHINEEHTPYEVFMFLNSKVETLDSIQRKDGHLLVEILGALRRIDKEAYEAIRKPNEVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPI2</accession>
    <name>A0A133VPI2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPI2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="70" mass="7557" checksum="53B51A3B09269929" modified="2016-07-06" version="1">MKLLNLAFRIFGLAQKGLMKLSTRFLGMNLNSTTTAVGLFVLLFALIISGKLKWAGMVFIGLVLLGLTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPJ1</accession>
    <name>A0A133VPJ1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPJ1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="75" mass="8180" checksum="92BDF5C781A4B047" modified="2016-07-06" version="1">MNVMNVIEKETAKVFLGKEVYVTKTDGYTWVGELIKVTDTSIILESKKHGKSVLSLNRVEEMGEAKNSNGGGGYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPJ2</accession>
    <name>A0A133VPJ2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein eS27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps27e</name>
        <name evidence="6" type="ORF">AKJ58_00225</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPJ2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001629">
        <property type="entry name" value="PRK00415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB08333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="61" mass="6655" checksum="A398449E3834F2A8" modified="2016-07-06" version="1">MPKSKSQGPSFLRVKCTDCENEQVLYSHSSTEVECQICGKTLVKPSGGKAEIKAKILGEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPJ4</accession>
    <name>A0A133VPJ4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPJ4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="114" mass="13187" checksum="8BA3EDD730D408BE" modified="2016-07-06" version="1">MVRKKALRVIFPYALWKAQSGFEETGSGKMNLREIAKACASILCLDESRDKITERQVRGLCEEYERGGWIKQLTRFRFRTVYELDPEVAQILSKPTVAVEKAYEELKEEGVDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPK6</accession>
    <name>A0A133VPK6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPK6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="260" mass="30602" checksum="97A5F2A7935656EE" modified="2016-07-06" version="1">MKQRLTEEEVEEIFKVSKWKYEKMSEETGKILEESIDSKRMERVIKSTVASIEMRIKNRKEVVFKKRLYEDGWVNRDPYYLLCLKAITPAIYRIYAKGTSTYDIRTSYGPRLLNERIAEVIGEDNLKEIMSKFKEYGEKYTEKLSKDIVDKLNISYSNTKWRGVTKSIITDWLGKIRKQGIDPKDIDILKALLDMDKMSIKEIADNVPISYRTVRRRLDGSNILQKYVDSKKQGRKKVHWLNKEGKDEVKNIVKEIGGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPK9</accession>
    <name>A0A133VPK9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPK9"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="213" mass="24493" checksum="501EC7951CC9D7A6" modified="2016-07-06" version="1">MIKAKIKPFVAKSDTPDFFENLKGFLRNIVEGIPQNELQGKTKQLSLEDIEEFSERTVPETREEFGEWLVNQAESQLAERYPPGTLKGREKVIDELERDTAFKYARAVRQTEENIEGISEEYRDEALRGGEKRSDKMGELPENVENVSRDLQDWLDGLEGIERPEVVGISVPRTTMESHGLSDDQIREVKEAVGREVNTTEEADKIREIVERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPL7</accession>
    <name>A0A133VPL7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Predicted 3'-5' exonuclease PolB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPL7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019288">
        <property type="entry name" value="3'-5'_exonuclease_PolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10108">
        <property type="entry name" value="DNA_pol_B_exo2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Predicted 3'-5' exonuclease PolB-like" evidence="1">
        <location>
            <begin position="55"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="244" mass="27803" checksum="CE64AD1E57155473" modified="2016-07-06" version="1">MNYIALDIETAPLQISDEVVIDYQIRKRFKRNLHPAFARVVAVGLLLPGEDYPLTRSGANEINLIKSTWDSIRKTSWDKLVTWNGLRFDIPFLKVRSRLLGITPNISVDTSKYSSPEDSNHIDCMWLLQGECNEIPPISLEIAARLFGIKYDESKKLTPLEISECFEAGKYSSIEECCSEDVKATAKIFEKLLPTIGTKPLATKKQVSYIIDIAKKLNIELDRPEVESWSKKEASEWIDKHKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPL8</accession>
    <name>A0A133VPL8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPL8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="78" mass="8472" checksum="62A5C4F88EDB3911" modified="2016-07-06" version="1">MVNAYSLVSTEPGETSKVYEKIGKLKGVKSVEAVAGPYDLIARIEVDSLEELTRTLFANIRGISGVMNTTTLIVIEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPM2</accession>
    <name>A0A133VPM2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPM2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="171" mass="20377" checksum="FDB1C404A777D80F" modified="2016-07-06" version="1">MKFWEDERVRKIRRVIGLVFILISLWIFTFSYLPTRYEGKMVVNNPEGTNKSQIIIHFVPDTVVAYRKVSGCPLTEMEGFASQGGNEFWIHSYIPYNEIIEACKHETCHLHQEYRGEYTPRTFRADQKECFEAEENSTKYEYCRDVADRVLSHAFSFYFQKIKNRTMEVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPM7</accession>
    <name>A0A133VPM7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPM7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="85" mass="9501" checksum="40A79EB52FCB1B88" modified="2016-07-06" version="1">MTGKICAVPGCEEELSEEQVERGMIVCSNCEEAKMHLCESCSKRLSPRRIKNGAVLCQDCESGCYTSELNYELEADFSDQRPPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPN6</accession>
    <name>A0A133VPN6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc-hook domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AKJ58_00070</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPN6"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.15"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc-hook" evidence="11">
        <location>
            <begin position="354"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="176"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KXB08387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="790" mass="92381" checksum="9E0B826519F07D23" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN06</accession>
    <name>A0A133VN06_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000031">
        <property type="protein sequence ID" value="KXB07832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN06"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7216" checksum="8656AA03A4F685A5" modified="2016-07-06" version="1">MTAINKITVLGGSTGVSIPPSILDALDMERGDLVALSLVSQDAFQVMKIQPKEVDLNEYGLAEIRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN12</accession>
    <name>A0A133VN12_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carbamoyl phosphate synthase small chain</fullName>
            <ecNumber evidence="10">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Carbamoyl phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">carA</name>
        <name evidence="12" type="ORF">AKJ58_01715</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="10"/>
    <dbReference type="EMBL" id="LHYK01000034">
        <property type="protein sequence ID" value="KXB07807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN12"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.279"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 10" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KXB07807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="360" mass="39198" checksum="A35757EB668B0335" modified="2016-07-06" version="1">MKAVLGLEDGTTLKGKGIGAEGATQGELVFATPFIGYEEALTDPSYKGQILMFTYPLIGNYGISHEVMQSDNIKAEGLVVRSACSNPSHKKSDESLEEFLLEHDVSGIEGIDTRMLTIKIREHGTMKSALLVGDKNEKRAVELAKNQPDITERDLIPKVSCEKGYRIKGEGPRIVVIDTGVKRNILKSLEERGADLLVLPHDSTTSEIKKHDPDALFVGNGPGDPSRAVGPIRAVKNFFGEIPIFGICLGHQIISRALGGNTYKLKFGHRGANQPVRDIESGKIYITSQNHGFATDPDSLDGTDLKITQTNANDNTVEAIKHESQGVFAVQYHPEAFPGPRDSIRMFFDQVVEKAGGNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN15</accession>
    <name>A0A133VN15_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000030">
        <property type="protein sequence ID" value="KXB07842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN15"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="147" mass="16809" checksum="05CE3F7B172B0281" modified="2016-07-06" version="1">MQVENPLTESGREFKEGCEEIDGEFHQEEGKLLFCRTDNFNGIFAPSPSWDEPVLVLGPDDTITGIEKVRSKEDTLEVLTPTKIISLQEGGDRETRTRTYIPAVAMAEMGKLEAEMFEKKDKSLQEILDEHEWISDLQKEEAIEKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN22</accession>
    <name>A0A133VN22_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA (cytosine-5-)-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000028">
        <property type="protein sequence ID" value="KXB07858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN22"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB07858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9084" checksum="B78DD289C5AC6140" modified="2016-07-06" version="1" fragment="single">MKVISLFSGAGGLDLGFKKAGVDIVYANDIQEDFCETYKNNIGNEIVCENIKDFHTSSTSLNSLPSIIIGEILTLPSLSSFLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN39</accession>
    <name>A0A133VN39_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000029">
        <property type="protein sequence ID" value="KXB07849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN39"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="275" mass="32697" checksum="7F9C371F081D4FC4" modified="2016-07-06" version="1">MGDCVGHAEESRIVKRRLYCDNCRQHLEKSVYIVVDGGILLGSTETREEAEKLLTAVNKEKLTLWYHDLYQKYVKEQRDKHMYKNFLRNLREWYGREAPGELKSKFERFRFLRNWRHLKSGDSPVESIENFLTYRKLLEALDKLQEDGRETLEIYYLEEVSEGEEEWAVDILQDELNKRCDTNWTWTVITRKQLEAEDTEEEELPEWNIVVEEPFSEEVVAKAVKKWLWGRRYDFEIKMIAPEEAKGSGILEKLREKGSKLEVSGYESLSRKGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN49</accession>
    <name>A0A133VN49_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000028">
        <property type="protein sequence ID" value="KXB07859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN49"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9566" checksum="0B585FA2872854B9" modified="2016-07-06" version="1">MDWDKYGFVSASKYRKKVVLALQKKPKTPKEIEEEVGYHLSHVSNTLGELSDEGIVRCLTEKRSKGRVYGLTKLGEEIAQQLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN52</accession>
    <name>A0A133VN52_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000030">
        <property type="protein sequence ID" value="KXB07841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN52"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="126" mass="14990" checksum="39021C89DAED2945" modified="2016-07-06" version="1">MQRKGLNYKVGHIICKWHILEYIKRIERLPDTQLPTSHNIANYFGVATGRIRNQLDNMEGSGWLRKREEEKGKRKIYRWELGEKSWKFIEGFRPLGSNGFLSDKNPFLDKVRKRAKKMGKSFDQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN54</accession>
    <name>A0A133VN54_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase RgtA/B/C/D-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AKJ58_01545</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000026">
        <property type="protein sequence ID" value="KXB07882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN54"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050297">
        <property type="entry name" value="LipidA_mod_glycosyltrf_83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908">
        <property type="entry name" value="MANNOSYLTRANSFERASE YKCB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908:SF11">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase RgtA/B/C/D-like" evidence="9">
        <location>
            <begin position="90"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KXB07882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="511" mass="58290" checksum="4D5520E0611E97BB" modified="2016-07-06" version="1">MLGYWYQFLLRTEIRGANKILNRDKLENYLPIIIVLLIFFMLWLLLFLSPWKYLARVSEERWWMYITLNYKRYGLLTPVGPYGHLVHFSPLYSWANYSMISITGWAEAGRIVSLFFGAGTLLLMYNISKGWYDQRTGTLAMFFLAVSPLFLQGSVHIKRATTMLFFSVLSILFLMKYLKNERNRYLLATGIAVFLSAFSKEVGIFTFFVVGGYLLYKKRFKILRNPMVYVAALIPVLLMFSWYLHIESIPTSVAPAPPAGQISDRIFFNLDAPLFRVLSETAVELAVAIPIATLGLALVGVGQWKEKDRLILFWLLAGVLFYLVFLQGSFHHTHYSTFMLPPLAIFSARGTITLSKKLSSLLKIGENLAPLTVVLIVLLLTAGGFGFLYFSLLRPNEAYEKEIRLIGSYVEKNAGEKGLIAIDVWPVYYHAFPIDINRIKTIHSPYPETIDNLSERPKIIVLNVKPHPDLGWMGRTNTVASESEVGEILSMDNYQMSKALTYFYVITSGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN55</accession>
    <name>A0A133VN55_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_01605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000028">
        <property type="protein sequence ID" value="KXB07860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN55"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="6">
        <location>
            <begin position="57"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB07860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="282" mass="31732" checksum="071FC69C511F48DE" modified="2016-07-06" version="1">MGFELRFELLKEIAEHVELKDILLIVSVPVVAILVFLLPDSTQEALVLNYKDPSPVTLFTKTFVHRGVDHLKNNLTAYIFFAVPSFLMSALTRQKRRFYLCFLLFVLVFPFILSSLNLHFIDAETGRGLSGVVSAFLGFMPVSVYLFLKEKMAEDLKISFSVSLVLFALGSIAFLFVGPLAQKLLWLVIIYSLAVFTLWSSTREVDFGKLIESLKGELARQNYLLFSTVSVLVFLLSVPLLFPVELVRGGGFVNVLAHYFGFALGFFVPYLAVSLHEAKRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN63</accession>
    <name>A0A133VN63_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 17">DNA polymerase II large subunit</fullName>
            <ecNumber evidence="17">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AKJ58_01530</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 22">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="17"/>
    <dbReference type="EMBL" id="LHYK01000025">
        <property type="protein sequence ID" value="KXB07892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.211"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056172">
        <property type="entry name" value="PolC_DP2_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056171">
        <property type="entry name" value="PolC_DP2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210:SF1">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14890">
        <property type="entry name" value="Intein_splicing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24846">
        <property type="entry name" value="PolC_DP2_cat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24844">
        <property type="entry name" value="PolC_DP2_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="19">
        <location>
            <begin position="1078"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="NCBIfam" id="TIGR00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KXB07892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="1595" mass="178503" checksum="F1385F6AD4698CE5" modified="2016-07-06" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN64</accession>
    <name>A0A133VN64_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CopG family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000029">
        <property type="protein sequence ID" value="KXB07851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN64"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="72" mass="8197" checksum="9EB13B45C43873F5" modified="2016-07-06" version="1">MEGKFTTVSIPIQLFNEVKEQIEGTGFTSASSFVAYILREIIIEAHETGEMPFTRDDTEKIKKRLKALGYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN74</accession>
    <name>A0A133VN74_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000024">
        <property type="protein sequence ID" value="KXB07902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN74"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="73" mass="8679" checksum="25EB556F74B942E3" modified="2016-07-06" version="1">MARGRRCEGCDNREFRLEPNRQGRGFKVYCTECGRQQELRAVPKISWTPLKEDDLRKLEELRRTVLANLTQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN88</accession>
    <name>A0A133VN88_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000024">
        <property type="protein sequence ID" value="KXB07906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN88"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="76" mass="8701" checksum="353A5D66452674CC" modified="2016-07-06" version="1">MVEEESHRPLGDIFCPECGSSRLYYFLGLKAGHIYVCKDCGYHGPVVIEGSEIAEELQRKRIKEIKEKRSKANNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN90</accession>
    <name>A0A133VN90_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000026">
        <property type="protein sequence ID" value="KXB07881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN90"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB07881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="183" mass="21224" checksum="C9571F968985339A" modified="2016-07-06" version="1" fragment="single">EKKPDHLVKSFKKVSEENPEAKLYLVGGRGGKNEKRIRKLVKELNLSKNVEIIERVSRKEVVKWMNKCQVFAHPMPTGSFGMVTLEAMACGIPVVATKTPYSPTFLGDSGLYVEPNDEEEFAEAILTLLEDEKLRKKLVKRGKERIQKFDYSSWGRKMHRIIRNSRNENLPKKRSDPDWIFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN93</accession>
    <name>A0A133VN93_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_01550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000026">
        <property type="protein sequence ID" value="KXB07883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN93"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000390">
        <property type="entry name" value="Small_drug/metabolite_transptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30561:SF9">
        <property type="entry name" value="4-AMINO-4-DEOXY-L-ARABINOSE-PHOSPHOUNDECAPRENOL FLIPPASE SUBUNIT ARNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30561">
        <property type="entry name" value="SMR FAMILY PROTON-DEPENDENT DRUG EFFLUX TRANSPORTER SUGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB07883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14259" checksum="21019C57031767B5" modified="2016-07-06" version="1">MFNGPSSESETSMMLWIYVTLAALSNGSAQLLLEQGMRDFAVQSLGTSSLPIYLFKMLTTPLVLAGIIAAGVSMLIWLKILTYAGVSYVFPIWMGLTFALVLVGSSILSGEAISALRIMGVVIIVTGVFITAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNA1</accession>
    <name>A0A133VNA1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="6">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thi4</name>
        <name evidence="7" type="ORF">AKJ58_01375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hydrogen sulfide + glycine + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="6"/>
    <dbReference type="EMBL" id="LHYK01000021">
        <property type="protein sequence ID" value="KXB07945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNA1"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.175"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB07945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="262" mass="28501" checksum="0167B1600B3E1477" modified="2016-07-06" version="1">MESFLPTNEKDITRAISKSFFREFQEYAESDVIIVGGGPSGLMAGRELSDRGVKTLLIEANNYLGGGFWIGGYLMNKITIRDPAQSYLDDLDIPYEEASDNIFVADGPHACSKLITAACDAGVKILNMTRFEDVVLREDNRIGGVVINWAPIKSLPREMAYLDPVGLEGRLVIDATGHEAGVVRKMEERGLLETKGEGGMWVERSENLVVKHTGIICPGLLITGMAVATVYGLPRMGPTFGAMLSSGERAAKIAYEELGKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNA6</accession>
    <name>A0A133VNA6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000025">
        <property type="protein sequence ID" value="KXB07891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNA6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="95" mass="11007" checksum="AE268005351741CA" modified="2016-07-06" version="1" fragment="single">SFFDKSEDDWSKKIPEYSPEEDSKALEKNAERVMETGEPRREDHGTELRRTTSCACCGRNRLEHPYRPRGDAWPPQAGLTELLSMVLAWRGVERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNB8</accession>
    <name>A0A133VNB8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="9">1.1.1.302</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_01425</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HTP reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="9"/>
    <dbReference type="EMBL" id="LHYK01000022">
        <property type="protein sequence ID" value="KXB07934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNB8"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.188"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050765">
        <property type="entry name" value="Riboflavin_Biosynth_HTPR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011:SF7">
        <property type="entry name" value="2,5-DIAMINO-6-RIBOSYLAMINO-4(3H)-PYRIMIDINONE 5'-PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_8G06820)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="Bacterial bifunctional deaminase-reductase C-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB07934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="223" mass="24383" checksum="104E639540CF7513" modified="2016-07-06" version="1">MGSPYVILNAAMTADGKISTRGGDSRISCEEDLDRLHRLRSEADAVMVGIGTVLEDDPNLTVRRVKGENPTRIVVDSRARTSSEAKILNKEASTIVAVSNRAEEREVNRLRSLGAQIIAAGDVRVDLVHLLEQLHEREIRKLLLEGGSTLNWSMLREGLVDELLIAVAPRIVGGKGAKTLVGGEGFKKVSEGIKLKLMRYETVGEDLLLIYKVKGSARDSENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNC3</accession>
    <name>A0A133VNC3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000023">
        <property type="protein sequence ID" value="KXB07917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNC3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17571" checksum="6EE792B19D72B83F" modified="2016-07-06" version="1">MDEFPEVSPDSLEIVRAITDLKGTVWILNGRENQLIEFIKENAPALLKRRRYTLELEPMDLEEVRELLIFRMAWARGGNYDERIIEPFLPETIEKIYKRSEGVPREALKLASNAVYNAIKEEKGEITPELVFEGRKKKKSFWSFLPFIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VND3</accession>
    <name>A0A133VND3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conjugal transfer protein TraB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000022">
        <property type="protein sequence ID" value="KXB07930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VND3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF7">
        <property type="entry name" value="TRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25399" checksum="E67FC14F3F219D63" modified="2016-07-06" version="1">MIRRINDRLILIGVAHVLPESVVEVRETIQEENPNVVAVELCPARYFALTEKERRGSGVNASGLEAGILNWVLHFFQQRMAQQAGMIVGGEMLVAVRTSKKVGARVELIDRDMDITLQRFINRISQWEKLKFAFNVLFGLLVGEKIDFRKLTDEEVVERLVSELKEASEKAYEVFIRERDEFMAQRIVQLMETTNGKIVCAVGAGHVPGLYKRLDPVIQRGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNE2</accession>
    <name>A0A133VNE2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-aspartate dehydrogenase</fullName>
            <ecNumber evidence="6">1.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nadX</name>
        <name evidence="9" type="ORF">AKJ58_01450</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-aspartate + NAD(+) + H2O = oxaloacetate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11788"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-aspartate + NADP(+) + H2O = oxaloacetate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11784"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.21" evidence="6"/>
    <dbReference type="EMBL" id="LHYK01000022">
        <property type="protein sequence ID" value="KXB07933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNE2"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.192"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01265">
        <property type="entry name" value="NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022487">
        <property type="entry name" value="Asp_DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020626">
        <property type="entry name" value="Asp_DH_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011182">
        <property type="entry name" value="L-Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03855">
        <property type="entry name" value="NAD_NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009828">
        <property type="entry name" value="PRK13303.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009829">
        <property type="entry name" value="PRK13303.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009830">
        <property type="entry name" value="PRK13304.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31873:SF6">
        <property type="entry name" value="ASPARTATE DEHYDROGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31873">
        <property type="entry name" value="L-ASPARTATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="Asp_DH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005227">
        <property type="entry name" value="Asp_dh_NAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="10"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate dehydrogenase" evidence="7">
        <location>
            <begin position="175"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB07933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="276" mass="29476" checksum="4E33E549A47BBE29" modified="2016-07-06" version="1">MCQRIVAIIGCGAIGSILANAIDKKRTGNSDLRFLFDMNFERAKDLADKLDGPTKVAKELNEILEDKSVDLVIEAASQEAVIQDSADVLRSGKDLMVMSVGAFSDSDLLKEVRKVAEEAGKSIYLPSGALVGLDGVKAANIEKIDEITLVTRKPPEVLSTTKYVQEQNIDLSNLDRPQTIFESSAKNAVKAFPESVNVAASLSLAGVGFEKTKVKIIADPSLTSNVHEVNVKGEVGELSTKAKNVPSPDNPKTSYLAALSAIMTLRSLTEVISVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNF4</accession>
    <name>A0A133VNF4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH dtxR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AKJ58_01335</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000019">
        <property type="protein sequence ID" value="KXB07978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNF4"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.148"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB07978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="145" mass="16545" checksum="C31D21B46BDD6B91" modified="2016-07-06" version="1">MEIDLNPEERSESVEDYLEALWVSEEEEKPLAKIKWISSHLDVAPPSAVEILKKMEKQGYVDYETRKGVKLTKKGRKIARQTIRNHRLIETLMKKTLGQEVDEEVACGIEHHLTKEFADALCTELGHPRKCPHGNPIPKGDCCPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNF8</accession>
    <name>A0A133VNF8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNF8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="127" mass="14316" checksum="2EC5639BAAB866D2" modified="2016-07-06" version="1">MSKGGSTSGLGRRVARLTRKLLKRATGGGKVSPERAQRIRREVADRVASHLLEELAAEDPFYNVEYDETLAKVLRRGVRKFARKQALKSIAVEEEGGKARLEFRRADLPDGWEIYRTVSDAVDEIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNH4</accession>
    <name>A0A133VNH4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Adenylate kinase</fullName>
            <shortName evidence="4">AK</shortName>
            <ecNumber evidence="4 6">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">adk</name>
        <name evidence="8" type="ORF">AKJ58_01345</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="4 6"/>
    <dbReference type="EMBL" id="LHYK01000020">
        <property type="protein sequence ID" value="KXB07963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNH4"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.151"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001380">
        <property type="entry name" value="PRK00279.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001381">
        <property type="entry name" value="PRK00279.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="2 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="7">
        <location>
            <begin position="122"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="4">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="4">
        <location>
            <begin position="121"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="131"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB07963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="214" mass="24384" checksum="305CB670DB63FBB3" modified="2016-07-06" version="1">MNIVVLGPPGAGKGTHSSRISDRYDLIHITTGDIIRDEISRETEIGRKAKKYVQSGKLVPDEIVIEMMRKRLARKDCKDGFVLDGFPRTVNQAEALDEITDIDVVINLGVPKDVLIKRLSSRRICRDCGEVYNLVSMRSEKPGVCDECGGELYQREDDRPEVVEKRLEEYDKQTKPVLGYYRKKSKVKDVIIKKEEPVEKIVQMVFDALDETVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNH5</accession>
    <name>A0A133VNH5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNH5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="158" mass="18742" checksum="24C699981D0AF89F" modified="2016-07-06" version="1">MKGRIEQLSEQDRKTIFELINRAAKRYRGAVPEDCWSEPYMPGEELSEEMRKMKFYGLRAQKLLGVVGVQNLSGVTLIRHLYVRPERQGIGTKLLNFALDRAHNEKVLVGTWRDASWAIKFYERNGFLNLGNQSRFLRRYWEIPERQVEESVVLRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNI0</accession>
    <name>A0A133VNI0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000019">
        <property type="protein sequence ID" value="KXB07977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNI0"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.147"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="149" mass="16951" checksum="F583E2D7643D6771" modified="2016-07-06" version="1">MKGVLGQVNETQSTEKSDEVIIRILKENTNITVVGCSRTEGKPAHEIPKFLKSKGYRIIPVNPFADEILGEKTYPSLEEVEEKIDVVEIFRPSEEVYEIVEEALETDAKVIWMQLGIKNEKAKKLAEENGLKVIQDKCMKVEYKRLIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNI8</accession>
    <name>A0A133VNI8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_01185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaB/Mog family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNI8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232:SF2">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="17"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="172" mass="18821" checksum="C1CB818C0DEBEDDB" modified="2016-07-06" version="1">MSAAEHKERAPERLHVGVITVSDSRAAAMKEGRDEDISGRVIEEELAKEGHSSDRTILFDEEDEIREKIEELVSNPEVDAIITTGGTGIARRDRTVDVACSLLNKELPGFGETLRRIGYEEHIGGPALLTRATAGIISRVPIFCLPGAPNSVRVAMKLILPELPHVVKHARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNJ4</accession>
    <name>A0A133VNJ4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="6">IGPD</shortName>
            <ecNumber evidence="6">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hisB</name>
        <name evidence="7" type="ORF">AKJ58_01215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="6"/>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNJ4"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.115"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000003">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002111">
        <property type="entry name" value="PRK00951.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="190" mass="20845" checksum="EAE09C8E843B14ED" modified="2016-07-06" version="1">MRKSKIKRETKETTIDIRIDLEGTGKAEVQTGICFFNHLLESFTKHGRFDIKAKSLGDFKHHIAEDTMIALGIALNKALGDKKGIRRMGDAIVPMDDALALVAVDLGGRVYSKVEVDFTKKRLNDLNSDLLVHLLETFASNAKLNLHVKLMRGDNDHHKAEAIFKALGVALSEAVSLTGVDEVPSAKGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNK1</accession>
    <name>A0A133VNK1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNK1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="228" mass="26117" checksum="71B5D19A60433A01" modified="2016-07-06" version="1">MRVSFMGILFLTNCTNQKWPDELYNKGEIRREMRNRYGFSEIPGCDVRGNFPSQRDPPFVAPAKEMYRGSFSSSLKPFAERLSDADLCFISAGYIFVRGIDLIYYYDCRLSGKGGKRKRLASELTDEVKEKFRRRVSDGSYGTILIVLSGIYFETLLGDHPTFLHIINEESRGELPQVFVAGKRSKLVEIKSINEDLDLDVSTFRRKGFAPLTNAQKQGILDEIEQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNK5</accession>
    <name>A0A133VNK5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNK5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="77" mass="8740" checksum="1455B95177FD9E50" modified="2016-07-06" version="1">MTLTGDGKFIFNLEEEGETVLACDNCQCVNWGVKPPKEEREEKEELVYLCTCKNCGERKVLFLGPENSENSHKTKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNK8</accession>
    <name>A0A133VNK8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNK8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="79" mass="8924" checksum="1541C3B789EE8E35" modified="2016-07-06" version="1">MEEKLEVGEGLASQVLHRHVGFHWSISIQNGKSGERIKSKVSGHNDTALAMFQSLGEARKKLTKQIKETHEELTKNEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNL2</accession>
    <name>A0A133VNL2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4Fe-4S domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AKJ58_01170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNL2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.40">
        <property type="entry name" value="Electron transport complex subunit B, putative Fe-S cluster"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052539">
        <property type="entry name" value="MGD_biosynthesis_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072:SF1">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="5">
        <location>
            <begin position="147"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB08007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="244" mass="25819" checksum="9F5C5B93AA6871AD" modified="2016-07-06" version="1">MLRVVGIAGYKEAGKTKVVEGLVKELVDRGYSVGTVKHVPKGGFTLDQPGTDTWRHAQAGSEMVVCLSPEELAILERRKADMDEVLLKMLDLDFAVLEGFRESEGLAKIMVARSDGEAVDLADEFTVGFIGHGTGDRPVLKRGDFSALADLVEKRAIPPVGGLDCGACDYSSCRDFALAAIIGDAPKDGCSAITGGVKLIIDGERVPLNPFVQNLIASTLAGMLSALEGGKGKKIDLEVTRSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNL9</accession>
    <name>A0A133VNL9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="7">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">ImGP synthase subunit HisF</fullName>
            <shortName evidence="7">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisF</name>
        <name evidence="9" type="ORF">AKJ58_01210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="7"/>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNL9"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.114"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB08013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="252" mass="26998" checksum="17DC8F4BBC3DEC4B" modified="2016-07-06" version="1">MDTKRIIVCLDTRHGELVKGVHFKDIQEIGDPAEYASLYEDQGADELVFLDIAATPEGKPTFLSAVESVCDRISIPLTVGGGIRTVSDAEAALDAGATKVSIGTAAVENHRLIEEVSAKFGKKGLVSAIDANRTSEDKWEVYVSGGRKPTGIEMVDWAKEVESRGAGEILYTGVHTDGTQEGYDLEGTRAIVEAVEVPVIASGGAGNLKHIEEAFTKGKADAALAASIFHYGTYTVREVKEYLQERGISVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNM1</accession>
    <name>A0A133VNM1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="7">PRA-CH</shortName>
            <ecNumber evidence="7">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisI</name>
        <name evidence="9" type="ORF">AKJ58_01195</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRA-CH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="7"/>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNM1"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.111"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.810:FF:000001">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000768">
        <property type="entry name" value="PRK00051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB08017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="119" mass="13873" checksum="0C8621D0C51284B3" modified="2016-07-06" version="1">MLDFKDGLISTVTRDYDDKEVLMTAFMNEEAAFKTLTTGLAHYWSRSRGELWLKGEQSGHHQKVREIRIDCDGDALLLDVEQKCGACHKGYRSCFYRRIEDGKLKKIMEKKFEPSEVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNM5</accession>
    <name>A0A133VNM5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="9">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_01100</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="9"/>
    <dbReference type="EMBL" id="LHYK01000015">
        <property type="protein sequence ID" value="KXB08048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNM5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.89"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02888">
        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050862">
        <property type="entry name" value="RdRp_reductase_class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02504">
        <property type="entry name" value="NrdJ_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371:SF1">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371">
        <property type="entry name" value="VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="7 9" id="KW-0170">Cobalt</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleotide reductase large subunit C-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonucleotide reductase large subunit C-terminal" evidence="10">
        <location>
            <begin position="206"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB08048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="394" mass="44019" checksum="49720D6E10AC7D25" modified="2016-07-06" version="1" fragment="single">DDFMESVQGDKEYSLVNPRTGSEVEKIGAMEVFEKICENAWRTGDPGMVFIDEINRHNPTPQVGRIEATNPCGEVPLLPNEACNLGSINLSKMVEDGGVDWDRLEEVSKRAVHFLDNVIDANSYPISEIEEMAKSNRKIGLGVMGWAEMLIELGIPYDSEKAIGLADEIMSFIAEKAREKSVELGRERGSFPNFEGSIWNEDFDFMRNATVTSIAPTGTISIIAGCSSGIEPLFSVAFMRKVLEGEKLFEVNPWFEKIAKERDFYSGGLIEEVARGTSVQEIDEVPEDVKETFVTALEINLEWHVRMQAAFQKHVDNAVSKTINLPSDASLDDVKRIYELAWKLNCKGITIYRYGSKSEQVLYAGGKEEETSEFNLAESEYAGGRMDELCLLCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNM8</accession>
    <name>A0A133VNM8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-glucan phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000014">
        <property type="protein sequence ID" value="KXB08062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNM8"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.79"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011834">
        <property type="entry name" value="Agluc_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052182">
        <property type="entry name" value="Glycogen/Maltodextrin_Phosph"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02094">
        <property type="entry name" value="more_P_ylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42655">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42655:SF1">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="207" mass="23737" checksum="21321787B503860D" modified="2016-07-06" version="1" fragment="single">ERVFTIGFARRAIPYKRANLIFHDTERLVQIDREIGDFQIIFAGKAHPKGEESKKMIKKVNDNIRDLKDDIETTYLENYNMDQAALLTSGADLWLNNPERGREACGTSGMKAACNGVPQLGTLDGWWIEGHIEDVTGWKIGSEPGEKKENDEEADANELYEKLENKIIPLFYSNKKKWTQIMRNAIAHNASFFNAHRMVREYVVNAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNM9</accession>
    <name>A0A133VNM9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AKJ58_01070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="LHYK01000015">
        <property type="protein sequence ID" value="KXB08044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNM9"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.83"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="7">
        <location>
            <begin position="20"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB08044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="400" mass="44039" checksum="D8B1CA5241760F17" modified="2016-07-06" version="1">MKKSVEKIRSDIPFLDSGIIYLDNAATTPTPEPVIEAMLEYYHEYSGNIGRGLHRVTKRATEAFESSREKIARVINSEPEEIVYSKNTTEALNLLARGLNLKKGDKVIATVLDHHSNLIPWQRIEKEAGIELEIIHKCPNFIVDPTAVEEAIDEKTQIVTMPSVSNAFGTRQPVEEVSKIAKENNALFSVDAAQSVGHMPVDVEEMGCDFLAAPGHKGLLGPQGTGFLYIREEMIDDIEPLLYGGGIVKAVEEHKCELVEPPQVFDAGTPNIPGIIGLGRAAEYVLDIGLEKIKRRERRLVDQILEINSIDNIEVYGPKSAKKRGGVVSFNLKGLDSHEVSSLLDEIGNIATRSGHHCAEPAMKYFNLNGNARASVHYYNTGGEVDKFIEVLTGVAEELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNN7</accession>
    <name>A0A133VNN7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GxxExxY protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNN7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="19311" checksum="A6758D556FB19700" modified="2016-07-06" version="1">MFGGGLFQEVVGLVDQWIPEKEYGHENKFQDVLGDFLRNKLRYQYQKRIPVNVRKNVHGIQVDVLVNKNIPIEMKRNLNKAQWRALDSEVGDYLNIYNSLIVVICGVEKEEVIDDLTNKYQGDQWGFTPQNLVVRVKRRKNYGKKPKKRTTPRRRIGFHVEDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNP1</accession>
    <name>A0A133VNP1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000014">
        <property type="protein sequence ID" value="KXB08058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNP1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="332" mass="37210" checksum="25E3A47B1FC10E66" modified="2016-07-06" version="1">MPQLLGLLVAYFQSQRKTEIGREAQTLADGISRASFSILSGGQKNYDLPVAVGGAPYRLTVDEDRNAVVVQITGGSQEGAEYGSVIDAYLQVLSIPDPGEVLYIAENGGRIVLSRSPLQIQEEEISSYPSLSPPDFYYFARENQREASALGASYFWALNHYLEEENLDVREYVWQNPDSNTLLTRVTSEAGFIVVVSVSGEENEANVGVVNRSWVVHRVENVKKDFDNGNSNPSIAEAYMNGWFYSRTQVVEYLQSRSWRRTDDNRAVEIPGSPEVYNAAATTNVGTYPTYLIEFTNQGENYLIHFQAMPWYYSENQAGFVFQSKPELEPIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNP4</accession>
    <name>A0A133VNP4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycosyl transferase family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AKJ58_01135</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNP4"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.97"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052078">
        <property type="entry name" value="Trehalose_Metab_GTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049438">
        <property type="entry name" value="TreT_GT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47779">
        <property type="entry name" value="SYNTHASE (CCG-9), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12100)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47779:SF1">
        <property type="entry name" value="SYNTHASE (CCG-9), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12100)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21269">
        <property type="entry name" value="TreT_GT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Trehalose synthase N-terminal" evidence="8">
        <location>
            <begin position="39"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="7">
        <location>
            <begin position="220"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB08030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="406" mass="46697" checksum="01C84B888E7C6138" modified="2016-07-06" version="1">MRKRLMPKTLADYRDIVGEDEISKIYRKSSEVLGKHILNINATYYGGGVAEILRNMVPLMNDAGVQVGWRAIPANPDFFRVSKKFHNALQGGDINLTEMKKEVYEGTIEEFARFTHINHDLVVVHDPQPLPLILYYKKRQPWIWRCHIDLSSPNEELWTYLKYFGIRYDNAVYHLDRFAWRGVGNYSIMKPSIDPLSIKNTDIPQSTIEKYLRKVGIKEGDSRPLISQISRFDRWKDPMGVLKVFDLIKNEMDCQLLLVGAIAADDPEGQEVYDEVFSEVQKREDVKIAVNVHDIVVNAVQRASDVVLQKSLREGFGLTISEALWKETPVVGSNLGGIPEQIIDGETGYLVDPYDYEDTAEKVMNIIGDENLSDEIGRKGKQHVKENFLIIRHIKEWLDLWTEFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNQ6</accession>
    <name>A0A133VNQ6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mechanosensitive ion channel protein MscS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_01140</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNQ6"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.98"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049142">
        <property type="entry name" value="MS_channel_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052702">
        <property type="entry name" value="MscS-like_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30347:SF1">
        <property type="entry name" value="MECHANOSENSITIVE CHANNEL MSCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30347">
        <property type="entry name" value="POTASSIUM CHANNEL RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21088">
        <property type="entry name" value="MS_channel_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel transmembrane helices 2/3" evidence="10">
        <location>
            <begin position="65"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="107"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="9">
        <location>
            <begin position="180"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB08031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31139" checksum="4E332A8657A647A6" modified="2016-07-06" version="1">MILDILSMELFTFAGAKITILNLLLFFIVIIGTFIISKYVIRKILDSILEKSGIKKEARFTLLKVVHYLTMFIGFWVAFNILGVQLTALLAVVGIAGIILGFGLQPIIANFISGLILMGERTVQVGNWIEIDGRYGKVIEAGVRASTIRTMGNLHVIVPNRAFVENAFVNYSHGDKRIRIDVPIGVKYGSDVEKVKNVLLKIAEDNEDVLDNPEPIVYFKEFANSSLNFELKCWVRTPRYRPRVLSELNFEIDRRFAEEGIEIPFPQRDVWMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNR5</accession>
    <name>A0A133VNR5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AKJ58_01045</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000014">
        <property type="protein sequence ID" value="KXB08063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNR5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.78"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000160">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000083">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
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        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
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    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
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        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF21">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="697"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="777"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="806"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="876"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="12">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KXB08063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="900" mass="98993" checksum="54A926FC5A932DAB" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNR6</accession>
    <name>A0A133VNR6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00945</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNR6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF87">
        <property type="entry name" value="TRANSPOSASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="5"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="112"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="43874" checksum="3B22148CB571F0E4" modified="2016-07-06" version="1">MENPKTMSEGCLIESDLLIELKADGLKNTSLKNYKIAVESLKMLDKDPRKMDKGDLVKWSAELNDRYSESTAKSYRTYVKRYFKWLKKGDLNGNNYPECVEWIKTGGKKRRLPKEILSREEIARMAEAAERQRDEALVWVGYESGCRPGELLGLKVRDVEFDEYGGQIMVDGKTGERRIRLVESAPQLKDWLFEHPKGNDPDAPIWLSGKGGSLGRWSWSRILKKLAEKEGIEKRVHPHLLRHSRATHLAAHCVNEAQLREIFGWTRNSNMPAIYMHLSGRDTDTAILNLYGIDSGSEKNKFNDGSKRRKCPRCGKRNPEEANFCNECGEKLDYPQGKRGSKPEPSSKGGEDKLVARIGRKMMEKFPEVVEEIVQEEKLAADSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNR7</accession>
    <name>A0A133VNR7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M10 metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AKJ58_00930</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNR7"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.47"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M10 metallopeptidase" evidence="7">
        <location>
            <begin position="454"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="234"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB08099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="620" mass="69098" checksum="2939E0F7D9FE76D3" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNS1</accession>
    <name>A0A133VNS1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent DNA ligase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNS1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF7">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="2">
        <location>
            <begin position="9"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="207" mass="23948" checksum="1225994B6DD16A3C" modified="2016-07-06" version="1">MRSLGIEELRDRIGKLESELSSLMFTRRIRSGNVDEIEKFYEECVERGNEGIIAKNLESKYHPGERGKDWLKLKKAGETLDFVVTRAEYGHGKRHKWLSDYYLAAYDENEREFKEIGKTYKGLTDEEIREMTKKLEKLRVSESGRTLKVEPKIVLEVEYSNIFSGESSSYDAGYSLRFARIKGIREDLNPEDASTLSKVSELAESEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNS4</accession>
    <name>A0A133VNS4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="9">PTH</shortName>
            <ecNumber evidence="3 9">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pth</name>
        <name evidence="10" type="ORF">AKJ58_01035</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 9">Belongs to the PTH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="3 9"/>
    <dbReference type="EMBL" id="LHYK01000014">
        <property type="protein sequence ID" value="KXB08061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNS4"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.75"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00628">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034759">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KXB08061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="120" mass="13268" checksum="18E93D4168087FE8" modified="2016-07-06" version="1">MDDHKQVIAARDDLGMSSGKLAVQVAHGSVGAAEKAMKEKKEWFEKWTDENRKKVVVRVSNEDDLRRLRDKAKDLGVPYEFVEDAGLTELPPGTSTVLAVGPAPDRIVDKITGDLPLWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNT5</accession>
    <name>A0A133VNT5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH asnC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AKJ58_00855</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNT5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="9"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KXB08115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="154" mass="17096" checksum="B43A691EEED5AB8A" modified="2016-07-06" version="1">MKGEKILKLDATDKKLLNLLQRNSKQSYADLAERLEISSSGAHKRAKRLAEGGVIKRFVAVVDPLKAGKRIKALIGVSTKPGRCSDVRPRLIERPDVLEVHETAGEHDLSIKLITKDTQTLNKLIHEIDRIPGVDSTRTSIVLKTEKETTAIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNU4</accession>
    <name>A0A133VNU4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNU4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="28254" checksum="C75DF117DC699BF3" modified="2016-07-06" version="1">MVVVLAIDALEYELVEEFDCKNLKQAVYGKTNISEFTQPRTMVLWCSFMTGENKEREILDKGDKEMWKTKIPPEKTFFSEFEDPKIIDLPGFSYDKKQHDRERELLKKFFEGAENQGEKKEVRKEYNKHGLQHHRKIKGEFLKALKENHDLLLGYFSAADVIGHLNFGNKTLMKMVYEDLDEIGGEIKEIRDDHLLVLSDHGMKGLGMFGDHNEYGFWSFDEKCNLEGPKLTDFADFLVDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNV5</accession>
    <name>A0A133VNV5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein AKJ58_00795</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNV5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.397"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00303">
        <property type="entry name" value="nicotinate mononucleotide-dependent phosphoribosyltransferase CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003372">
        <property type="entry name" value="PRK04447.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811:SF1">
        <property type="entry name" value="UPF0284 PROTEIN SLL1500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="Nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="364" mass="38079" checksum="A327F7A3F29E0C87" modified="2016-07-06" version="1">MGDEVKASKFIDNLKGKEPVFICTISNTMTAMVPGITVAGANPEMIKYTPPADVELVYHGECRCIPGVPATPDGKPTPAIITMSVLKRTEIPFFAVEAGCEVKPCAPHISLNIPDPGDNIQTGRAMDRTVVENGVESAKLIGKNFAELADYLVLGESLPGGTTTALGVLMGLGIDARGKVSSSMPKNPHEIKNRVVEEGMKAAGIQMGGLRDDPLGAIAILGDPMMVGVVGMALGAAPKVPVILAGGTQMAAVVALISRIKPNILGNVAIGTTKYIVEDRTSSIEWLIGQSADIPIFGADPGLGKSRKDGLRVFESGFVREGVGAGGATLTAMLKSEGEITTDIIRDEVEKNYEEVIESKLGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNV6</accession>
    <name>A0A133VNV6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNV6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="93" mass="10782" checksum="5B972A43591E4580" modified="2016-07-06" version="1">MRAGVKRVENLPIPVEELGTFEEPIKEIDRFPIFKILLESDARLEEPLSYLSTRVGLIHEKWSEMSSDGSLNENMLEKAVQEARKELKKKEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNW9</accession>
    <name>A0A133VNW9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="7">AGPR</shortName>
            <ecNumber evidence="7">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="7">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argC</name>
        <name evidence="10" type="ORF">AKJ58_00825</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>N-acetyl-L-glutamate 5-semialdehyde + phosphate + NADP(+) = N-acetyl-L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="7"/>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNW9"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.403"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23934">
        <property type="entry name" value="AGPR_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17895">
        <property type="entry name" value="AGPR_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000014">
        <property type="entry name" value="N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050085">
        <property type="entry name" value="AGPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338:SF10">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="9">
        <location>
            <begin position="2"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 8">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KXB08145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="340" mass="37050" checksum="1559C642F9B9A721" modified="2016-07-06" version="1">MSTAVIGVSGYTGGELLRLLINHSKTELVGVYGKKSAGKRLTELQPNLVGFEERTIKKPDYPEIGKNADVVFTATPHGIAMKFVPKILEGGAKVIDLSADYRLDDPKIYEKYYKKHESPELKAVYGLPELHRDEIKKAELVANPGCYPTAAVLSLAPLVKENMVKTDPIIIDAKSGTSGAGAKPSKMLHHPACAENIQAYSPTSHRHEPEIAQEIGKLVEDEVGIHFTPHLIPIVRGILSTSHVFMREPGAEEEVLDLYQKFYKNEIFVRVLKALPQVSSVVGSNLCDIGLKSDAKGERFVVISVIDNLIKGASGQAIQNMNLMFGFGEAEGLGEIPLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNX2</accession>
    <name>A0A133VNX2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">formylmethanofuran dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_00880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O = methanofuran + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="2"/>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNX2"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00980">
        <property type="entry name" value="FwdC/FmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017550">
        <property type="entry name" value="Formylmethanofuran_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03122">
        <property type="entry name" value="one_C_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673">
        <property type="entry name" value="TUNGSTEN FORMYLMETHANOFURAN DEHYDROGENASE, SUBUNIT C (FWDC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673:SF5">
        <property type="entry name" value="TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE 2 SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="269" mass="28750" checksum="5AAD3DEB608D9E7B" modified="2016-07-06" version="1">MKVTLRPKSKFTLPIDASCITPHEFAGKDLDEIAELKVFMGSAMSRLGDLFEIEGEFSESQEDVEIDLGGDTSMVRRVGEKMSDGLIKSLGGIGVRAGAFMSGGKVVFDEDAGPWAGQQMSGGELQINGNAGNYLGSTYRGDWIGMKGGKIMVKGNVGNEVGEWMNGGFIEIGGSAGLHLGAHMKGGVIVVHGDVEDRVGSQMVGGKIVVMGEVKSLLPGFKFEEEIKDIELDDGKFEGEFLRFSGDMAENGEGNLYVSKEKNEHLLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNY2</accession>
    <name>A0A133VNY2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flpA</name>
        <name evidence="8" type="ORF">AKJ58_00745</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNY2"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.385"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003277">
        <property type="entry name" value="PRK04266.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="86"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="130"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB08162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="226" mass="25744" checksum="FF16B65002F43706" modified="2016-07-06" version="1">MRVEEHESFENIYWIESEGKKRKLATVNLAPGNKVYGERLVKFRGTEYRCWDPYRSKIAAAVLRGIKDVPIKEGSRVLYLGAASGTTASHVSDIVGAKGIVYCIEISSRPLRDLLVVCENRPNMIPILADATKVEVYRAMIEQVDIVYQDVAHPEQTEIALENSLAYLKEGGHLMVALKARSIDVTKEPREIFQKEIERLERKMEILDSKLLDPYEEDHAMILLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNZ6</accession>
    <name>A0A133VNZ6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNZ6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24373" checksum="512DE093BC32CFFE" modified="2016-07-06" version="1">MSAEQKLKELPSYSEELGLNLREPEGRFKWFLASILFGKRISAEIAKRTFAKFESAGITTSEKILEAGWDKLVEILDSGGYTRYDFSTASKLLNISRLLEKKYGSLEKLYASAENNKDLEKRFLEFKGIGPTTVNIFLRELRPIWEKADPKVSSPALDVVKKIKLEENRKIKFLESSLVRVNLEYCKSKRCQGCPVRKSCSEFKNDNNRFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNZ9</accession>
    <name>A0A133VNZ9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="4">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AKJ58_00690</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the GARS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="4"/>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNZ9"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="116"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KXB08182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="470" mass="53136" checksum="1835E5217822242E" modified="2016-07-06" version="1">MERVGVLVVSYGSRASSMIDAFTRSQNYDVDIYVADKQRNPFNLEKSVKHIVIPDLDPENICKFVKKNQGEIDFGIVGPEKPIINGLRDLVEKKTNVPILCPTKKYAIEGSKVAQRHLFEKVVPEVNPRFKVFDPKDYKNVDDVKREVYSWLDELNNQVAIKPDAPAAGKGVGVWGDHFADRENLFQHFLSNYEYGPVIIEEKIEGEESSFQSFCDGKHLVPLPETRDYKRAFDGDEGDNTGGMGSYKDTGNLLPFMASKDREKEIKIVKQIFKEWKENGRNTGLIGMPFYVAFIHTAEGPMILENNSRPGDPEIQNIMPILKEDFVDVCFKMLDGNLTRVELEEKATVVTYKVPPSYGGFKDKFPKQVNNDEIGTPIDLSETKKLSQKYGNRLRVYPGSMELRNGKIYSRTSRTVCTVGIGDSIKEARAISLEGLQAMKGGALWNRNDIASEKHIQQSTKHLESLRNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP03</accession>
    <name>A0A133VP03_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AKJ58_00730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP03"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB08168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="265" mass="28676" checksum="AD7697C13F91C85C" modified="2016-07-06" version="1">MDNMEIIFSLIILAFLFDFMNCAAGMGLGTSLAPILFLLGYTPLQVVPVLLIAATVSGWVSGFFHHEFENVNFSFRPPLSKAAKVTLLIAGVGCIGIFFSVMLAYFLIKFPEVLIKTYVAVLVLFMGFIGLMSLKDIGDRKYRPKMLAGFAGLAGFNKGIGGGGYGPVVVMGEIFSGIYEKSAVGIVSLAEGIVSTVGVLTFFIIMAEGVAVDLWLLPSIFTGTFISSLIAPYMVRVFPNKVWRIIIPAYAFSIGAVFLMKIFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP04</accession>
    <name>A0A133VP04_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_00660</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP04"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.368"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="230"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="363"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="57481" checksum="2BCC9A1264C06155" modified="2016-07-06" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP09</accession>
    <name>A0A133VP09_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="2">N5-CAIR mutase</shortName>
            <ecNumber evidence="2">5.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AKJ58_00705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIR carboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="2"/>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP09"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.377"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 2" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="4">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KXB08184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="143" mass="15578" checksum="6E142D20571F0A7B" modified="2016-07-06" version="1">MGSRKDIEFAQPALDLLEELELDHEQKIASAHKTPEKVLKILKENEGKKTLYITIAGRSNALSGFVDANTRSPVIACPPYSEKFSGMDILSSLRMPSGVSPLVVLEPKNAALAAAKILALSDSELSEKIASYQQKIKEKIGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP13</accession>
    <name>A0A133VP13_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="10">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">purP</name>
        <name evidence="13" type="ORF">AKJ58_00710</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + formate + ATP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="10"/>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP13"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.378"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF2">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP biosynthesis enzyme PurP N-terminal" evidence="11">
        <location>
            <begin position="21"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="IMP biosynthesis enzyme PurP C-terminal" evidence="12">
        <location>
            <begin position="177"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KXB08186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="367" mass="41780" checksum="0E4812B8EC4BA73B" modified="2016-07-06" version="1">MIDKKEIGDIFSDYDEDNLTIGVLCSHSALQLVHGAKREGFRTLGICLKERERAYKSFPLASPDEFLILSDFSEILKSEHQRRMREENTVILPHGSFVEYVGPRNLVDKFRVPMFGNRASLEWEGDRGKQREWLKKAGVRVPKLYERPSKIDGKVFVKFAGAKGGRGFFVADSKANFEEGLEKRIKDGKISRQDAEKVSIQEFVPGVRYYPHYFYSLLDDRGAKVRGGAIELLGMDRRIEPIDESYRGLPNVPSEFFDYTVTGNEPLVIREKLLVDLIRMATGVVETSKELFPPGIIGPFCLETIYHPERGFTVFEISGRIVAGTNLYPEGSPYSPYFFEEPMSTGRRIAREIKDGVKKGRLGKILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP15</accession>
    <name>A0A133VP15_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP15"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="130" mass="14779" checksum="DCCA4F40F85A2D13" modified="2016-07-06" version="1">MWGDEHISRSKSEVASPIRLKVVLTKHAVRRLNERFFVKDQFQIESVFKCAVDGGFMFSNDDGTGEKLIEYGCLLVAGEFDGNGTFTVEEVYNLSHGISDRLKERMREGRPAPWESSKIVLPRENRGDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP16</accession>
    <name>A0A133VP16_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP16"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.386"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047099">
        <property type="entry name" value="Nop5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nop" evidence="2">
        <location>
            <begin position="261"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="383" mass="43126" checksum="F02A6D80F95F4D1B" modified="2016-07-06" version="1">MTIDLGECPLGVFAFNGGGKMIGYQLFPKDSKEIADRLYLIKNDKPTEEHQQLLKELYEEGHREFSMASEELASQLREDFDDVEFEIHVPSKGNLILRGSLSELSDKFDFGNFNGVLRDAILLVTRKDLRREASRRDKIVIEAIEMLDELDKSINTLYSRIREWYGIHFPELERYISDYPQYFKLVSELGARESFTESVLEEKGLSTEDARKIAKAAKNSIGTDFDDIDIEALKKVVKRVEKLYEARNSIEEYNDGLMSQIAPNIRAVVGSPIGARLISLAGGLKKLARKPASTIQVLGAEKALFRALHGKGKPPKHGVIFQYPDVKGLPRKQRGKAARALANKIAVAARVDVMSGRFVGDDLKEELEDRMSTIKSQAGFEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP28</accession>
    <name>A0A133VP28_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Integrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00590</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP28"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="30"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="136"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="37905" checksum="2387F8C27CDFAF82" modified="2016-07-06" version="1">MASRLESVQTTFRDFAGMKDGLKGSDEIIHKKGRLEEEYLEDCKARLSPHTVKSHECCLREFRGFLGKPVGKATKLDVRNFLNDLKSRGRARSTIAHKLAAIRSFFSYIREYHRVDTPDLSNVKVKDYPMSRWEGRGCVALEKSDIIKMLETVESLRNTLIIALLYFTGLRAHELAKIKLDSIDTEQRIIAVVGKGDKPRKVPYHPLLDRSLQLWIDRERKSYASSGRSPYLFPSKHGDRLTSNTIRRIVVAAAEEAGVQEIMGERADGGKIHKANPHAFRRSFATHGAESGIPLEDLRRMMGHSKQETTLIYIKESMERTFKSYHENFEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP36</accession>
    <name>A0A133VP36_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rad50/SbcC-type AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_00640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP36"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="3">
        <location>
            <begin position="5"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="219"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="374"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="666" mass="77809" checksum="4CCF94C276991023" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP50</accession>
    <name>A0A133VP50_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP50"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="209" mass="24159" checksum="00297C932D5612E0" modified="2016-07-06" version="1">MEGINVAKKSEIDFDVEFKEKDKELPVFLELSLSSWPLFLVNREYEGKITIANELNEPYPDDNHELVIEPSLSTERLDEERVLFLNISNIRAPGHFHISQLKANETRTLGFTISLRRRGNYNIKASCEEREIVKLDGSPQYREVHSGGMIGDKKFCAGEGWQVQYKSLNKGRRWDKLQFRCYSKIEFLLVLGSVAAILAALFSFLQFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP55</accession>
    <name>A0A133VP55_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Argininosuccinate synthase</fullName>
            <ecNumber evidence="5">6.3.4.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AKJ58_00530</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="5"/>
    <dbReference type="EMBL" id="LHYK01000006">
        <property type="protein sequence ID" value="KXB08225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP55"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="4">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KXB08225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="128" mass="14137" checksum="5A37605E1783301C" modified="2016-07-06" version="1" fragment="single">MKVALAFSGGLDTSVCLRILQEEYEAEVVTVTANVGQNPEKLKEAKEKAKELGAIKHFSFDLSKEFVEKYIFKSIKANGIYEDYPLSTALARYPIAAKLVEIAKNEDVDAVAHGCTGKGNDQFRFDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP66</accession>
    <name>A0A133VP66_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Haloacid dehalogenase-like hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP66"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.373"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3870.10">
        <property type="entry name" value="AF1437-like domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024196">
        <property type="entry name" value="NiFe_hyd_3_EhaR"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019370">
        <property type="entry name" value="EhaR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41410" checksum="B46BB3773F1DDB92" modified="2016-07-06" version="1">MNTQTLQDNRQKIFISDCEGPISKNDNAYELAVHFIPSGDKFFSLISKYDDVLADVVKKPGYRAGNTLKLIVPFLKAYGATEDKIWKYSAENILLVPGAKDTLQFVNTIMPSFIVSTSYKPYINALCHLTKFPQENTYCTELYIDKYEVTEKETEKLKKLRKEITNMSMIEIPKGADSLKDLPQGSRENVEKLNKIFWEKITKMDTGRAFQDVNPVGGGKKAEAVKDITGKLGGKIANVIYVGDSITDVEPMELVRENGGLAVSFNGNEYAIREAEIAILADNTIVTSILANTFNQFRREGVIQLANNWNRSGLKKHCADSRLVERVSELYPEKLPEVKIITDSNREELSEKSSDFRKSVRGEAIGNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP72</accession>
    <name>A0A133VP72_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP72"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="70" mass="7759" checksum="84B5C4D7087BF4E2" modified="2016-07-06" version="1">MISTEAGSMTDVYMKIKRLDEVQTAKMMTGPYDVMAMIEAKELADITGAVIEKIRGIEGVKETTTNIFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP74</accession>
    <name>A0A133VP74_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP74"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="155" mass="17260" checksum="E80DE89A84B82D66" modified="2016-07-06" version="1">MKEKILVGWDGSDQSRNALRYAIDEAKEKGLEKIAAVYVEKEESTRLRALLEDYKIGLSEEILKEEEVKINKLLEEAESIGKEKGLTVETHLLKNAHGPAVDIVKFAEKNDFKHIIVGSHGRSGISRIALGSVAEKIVEKGHCIVTVVRCNWPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP78</accession>
    <name>A0A133VP78_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_00410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP78"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041800">
        <property type="entry name" value="Hsp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="99"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="195" mass="22736" checksum="A89C6672165913CA" modified="2016-07-06" version="1">MLKRMRRRMERGFRGFPFFEEEEYERSLWEPFENMVKMFEGEIPEDLKDFVKEEETPSGKVRRFGPFVYGFSYSKRPGEEPQIQEFGNIRQAGPGRVVPASGGEREPLTEVVDLNGVYEITVEIPGVEKEEIDLSATEETLSIETTGERKYQKKVSFEEPVDPDKVDANFRNGILTIEVEKKEKEKKEKKKVEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP79</accession>
    <name>A0A133VP79_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AKJ58_00525</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP79"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46565">
        <property type="entry name" value="COLD SHOCK DOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46565:SF20">
        <property type="entry name" value="COLD SHOCK DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CSD" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB08265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="64" mass="7096" checksum="ED7F7A591D5BDCC1" modified="2016-07-06" version="1">MKGTVKFFSLIKNYGFIAPDEGDEDLFVHASDIESGSLGKDDRVEFESEEADRGPRAVKVVKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP80</accession>
    <name>A0A133VP80_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00605</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP80"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="252" mass="27867" checksum="E048E026549C7E9C" modified="2016-07-06" version="1">MRGKEISFDEDGIEQLLEDYSPSELDSQLSRVCPFFEDDTKEFTSVYGAIHRAVQSRLLVKLQIELDDLIVRGETEGEDGGRVDGLVGECKVSLNDRRVELRSSNGDEARDIAVIEVKTGNVSPWQAGAYAYRWNLPVIVAETKTGDAHLVDLETAVNLLAEAVDHQRDLEALGESGREIPDCYQCRDCGNQDCEYHRDGNRRTGNSVIRDKAKVLSNIDGVVEEIVSTIGEILESDGYTPVSDSGGEDQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPA0</accession>
    <name>A0A133VPA0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_00325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPA0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="171"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="347" mass="40189" checksum="028C608763ACD0BF" modified="2016-07-06" version="1">MSKEDTVSIQLPESLVRKAEDEGLNVSKTCELALERRLEALGEIDSNGANPSNSNSYNGNNNHVNGNNGNHSNHNHKVSFSKTDVQNFCNRLSAAGRSSLRVNAVKGKLEKLGAHVNWSCNLNDVTDYLNGRRKEVSNRTLSDDIQAIKQFFKDMNVYWIEKIKKPNLSKHRPKVVEKEDILNLMNELDHDKTEDKYVYRAQAAVHLSAVTGMRPWEIYRLSWEDVDIEERIIDLPAEKTKTKEERIVVFNERAQKRLRKLKKLFPDRPFINKAIYLITNKMDEKPELKLKHCRKFFSQEWDRRNGSTRIKEMLLGHFGSIDLRNYNGQTPDHLKDIYDEVGIKVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPA4</accession>
    <name>A0A133VPA4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPA4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021799">
        <property type="entry name" value="PIN-like_prokaryotic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11848">
        <property type="entry name" value="DUF3368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="148" mass="16569" checksum="2A37B70BF779EC7C" modified="2016-07-06" version="1">MRIITPSIVRAELEELSEYPDPEGKAAKKALELISEGLIITEEVEAEKEAQELLSPHVDEGEASCFICCRERGTRNLIMDDVNAATELEEKALRSGIRQKISVAIITELMKKGEISSEKARLAVNKLVKTRDWRGGVLEALVKKYFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPA5</accession>
    <name>A0A133VPA5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9 11">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="8">P5C reductase</shortName>
            <shortName evidence="8">P5CR</shortName>
            <ecNumber evidence="8 9 11">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">proC</name>
        <name evidence="14" type="ORF">AKJ58_00455</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8 11">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 11">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="8 9 11"/>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPA5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.325"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000190">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0521">NADP</keyword>
    <keyword evidence="7 8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 8 11" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="13">
        <location>
            <begin position="154"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="6"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KXB08253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="260" mass="27764" checksum="0C5673194F65CAE7" modified="2016-07-06" version="1">MKIGVIGCGNLGTSLLKGLLKAGFKGENIIASDLDERKLEELQKLGIEVTKDNNKTASDSDVIFLAVKPDIVGDVLEKAEIPKDKLLVSAAAGVSTDFLEDHTNARTIRIMPNICVGVAEMASCFTLGKRATEKDEEFVKSLLESLGTTFKVEEKLMNAVTGLSGSGPAYVYLVVEGLKEAGKELGLSEETALKLAAQTVKGSGEMVLNSGKDLKELIDMVCSPKGTTIEGIKVLKDRKVSEAFKKAVKAATKRSEELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPA6</accession>
    <name>A0A133VPA6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldehyde:ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPA6"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.311"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF8">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="103" mass="12237" checksum="FDAE65D2FE2AD5BE" modified="2016-07-06" version="1" fragment="single">VEEERWRQILSSLVVCFFARDIYKPEIVSDALNTVGFDLDKEDLNRIGKKIYQEKLKFKLREGFSLEELKLPERIFETPTPKGKLDEDKIKKAINYFKNEIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPB1</accession>
    <name>A0A133VPB1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPB1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="103" mass="12050" checksum="789CFF0204A55D39" modified="2016-07-06" version="1">MKVKGWNNGSPLETGAGYGIRISRKDREAYFRREWESVIIDLEGGSTIEVNISESFWRDCIELRSARIGKWMIRKELAPWEKNNPPSLELKHIEGRKFKLKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPB3</accession>
    <name>A0A133VPB3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPB3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039519">
        <property type="entry name" value="YokE-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14470">
        <property type="entry name" value="bPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon" evidence="1">
        <location>
            <begin position="3"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="YokE-like PH" evidence="2">
        <location>
            <begin position="62"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17937" checksum="00B5849D8607D86F" modified="2016-07-06" version="1">MPFCPECGTEVDGSANFCPNCGTGLEAKTSTPSIRKEEFKSAELDEMVEEIANELRDKGVIGPDEKILAYSRQSRYKSLIKPASLIVTEKQLVYYNPKIIGSEIKTRTTDEIHDIAIDAGIKNATITIRTEAMDDLVFENFPKNECNKVRNTIRRIAESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPC0</accession>
    <name>A0A133VPC0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CARDB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPC0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047676">
        <property type="entry name" value="FxLYD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038353">
        <property type="entry name" value="FxLYD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARDB" evidence="1">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="CARDB" evidence="1">
        <location>
            <begin position="103"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="383" mass="43083" checksum="9CEADBAE626C6A2B" modified="2016-07-06" version="1" fragment="single">GEEGVPAPAEFEVSNLTVTPSQVGPRESVSVSIEVQNVGDLSGDHKVELRVDENVEDSQTVTLEGGETTTVSFTIQRETKKSYSVSIGDLTQSFEVVTPAEFEVSNFTLNPEEPVVGEEFTASIDVKNVGELEGTYKAVWKVEGEVDDTHEVTLDPGETRSVTFSMIWNEAGTYNVSIGELEKNIEVKPVLKIIEHQKVAVEPYVRVWISGMLRNNSDNPLQPSVQVKYYDKEGYVIDTDSLSYSKIIPPKSEFPFRTRRIRVDDVEEIANYEIDASYQFYPNLNYLDDFTIIEDSRINNEVEVGFKNNSSEIADSVYLQVAFYNKQGEMLGVGVEVDPDTSAIGVPTKRNILPGEKRKLRLSFTYEEKREFSEYKLFISAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPC2</accession>
    <name>A0A133VPC2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPC2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="89" mass="10726" checksum="31D59D5FCB45A740" modified="2016-07-06" version="1">MSPIEEERILEYQASVDIVKEAVRACYQEEPTRFKDYELRDPGAFHELPICREISMRVEQPWAHTESEENHEKIKRIKEKINKKGKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPC7</accession>
    <name>A0A133VPC7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GFO/IDH/MocA-like oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPC7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="79" mass="8963" checksum="74F81F983D019C8E" modified="2016-07-06" version="1">MDLGREKSGGAIVDLGTFLIDLFLWFFNSDVRMVECRSGNFVFKDKETEDLALIMLKLKNGAFTSIDISRACPRPFQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPE5</accession>
    <name>A0A133VPE5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPE5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005368">
        <property type="entry name" value="UPF0175"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03683">
        <property type="entry name" value="UPF0175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="102" mass="11595" checksum="AD42DA6C78F6997C" modified="2016-07-06" version="1">MCVSETISTRVPEEIRREVEEFMREEKLDKSAAIRKLLKTGLLEWRKQKALTLLEKGKVTFNKAAEIAGMSVWDFADLLRKGGGNWITSLERIKSDIQAAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPE7</accession>
    <name>A0A133VPE7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_00230</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPE7"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.243"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000002">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003062">
        <property type="entry name" value="PRK03987.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003064">
        <property type="entry name" value="PRK03987.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB08319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="266" mass="30307" checksum="47FD8E02929AB9C5" modified="2016-07-06" version="1">MVRRKSEWPDKDEIVMCTAKKVFDQGSYVKLDEYGGKEGMIHISEVSSGWVKNIRRHIREGQKIICKVLNVDPKKGHIDLSMRRVKDSQRSWKSRQWKRDKKSEKLLEQVAKRVGSDLDTAYEKIGFCLQQKYDDIYSAFEEMAMKGKEAIDLPEVEEVWINALMELIESSVEAPYVEVKGYVDLRCSTSSGVEVIKSALSEARDSVTDSEVNVNVRYIGSPSYLIKISAPSYKVAERVLQETAEKAISTVEEAGGTGKFYTEREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPF1</accession>
    <name>A0A133VPF1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5714 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPF1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043768">
        <property type="entry name" value="DUF5714"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18978">
        <property type="entry name" value="DUF5714"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5714" evidence="1">
        <location>
            <begin position="50"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="228" mass="24601" checksum="CD5B4E2B839A8D2E" modified="2016-07-06" version="1">MICGGSLDYLSEPRSAECFYCGSEGKTRIICLEGHFVCDECHGKGAYEAIKEVALSSDSKEPISLAEDLMSHPSVPMLGCENALIVPASLMATLRNNGVEVSDEDLIEAMDRTQEQSKPPYCALTGACGVALGLGATISVLVGAQCPKDRPSAITMHSLARALETMANDVGPMCCKSFVRTAIGVAHSVFKEYLEIELPLRRDRISCVFTDKHPHGCRKSKCLYYSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPF4</accession>
    <name>A0A133VPF4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gins51 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPF4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21695">
        <property type="entry name" value="GINS_B_archaea_Gins51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054314">
        <property type="entry name" value="Gins51_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22090">
        <property type="entry name" value="Gins51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gins51 C-terminal" evidence="1">
        <location>
            <begin position="133"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="180" mass="21083" checksum="7042754DA60EDA51" modified="2016-07-06" version="1">MSDFGLGDIEKQWIEEKTSAKLAKLHEKFYERVADYVVGLSRELERSQDLRKELLREEFDWVIRMVQEIHFLRTLKAMELIVKGSLPSSLLEGERLRFEEIKDILKELREELLKTVVEGQAERPPHKKEKANVLLLISSELPQFVGDDMRRYGPFKSGDVVNLPKRSAEILRSHNLARRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPF8</accession>
    <name>A0A133VPF8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="10">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="10">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="10">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">aspC</name>
        <name evidence="10" type="synonym">aspS</name>
        <name evidence="12" type="ORF">AKJ58_00250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="10"/>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPF8"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.247"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="153"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="10">
        <location>
            <begin position="200"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="410"/>
            <end position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="10">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KXB08322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="439" mass="49916" checksum="2142B2E75BCA2CDF" modified="2016-07-06" version="1">MKPDPLGDWRRTHFSTEIVPELSGEEAVLMGWVTDIRDLGGIKFFILQDIRGSIQVTAPKSKVDSEILDKIDSISKESAVGVKGKARAEEQAPRGVEIIPSEIRVLNPAKIPLPLDPTGHQKADLDTRLNSRLLDLRRPKSQAIFKIKHAACQAIREYLISNGFIEIHTPKIVRSATETGATLFPISYFEREAFLSQSPQLYKEVLTGCLDKVFEFGPIFRAEEHDTRYHLNEVLSTDIEVAFVTKEDVMGVLEDVLVEVFKKVKQKCSEELKVLEKEIEVPEKPIPRITYDEALDKLEEAGKRVRWGEDFSIPEQRELGNIIDGAYFVSDWPADLKPFYIMAHKENPKLSHAFDLLYDEIELASGGTRVHQKEVLIKQLKNKGLSPKQFKYHLKNFEWGLPPHSGWGLGIERLTMALTGARNIRECVLFPRDQKRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPG0</accession>
    <name>A0A133VPG0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPG0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="60" mass="6473" checksum="24A74E4CC364620F" modified="2016-07-06" version="1">MEIAGKRTQSPVDSGITSNPLSSCAPFGQVWNYSPEKDYFIGGVLERKGKGGFFEEKGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPG1</accession>
    <name>A0A133VPG1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TNase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPG1"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.254"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="2">
        <location>
            <begin position="51"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="199" mass="22369" checksum="91F3AF0973545893" modified="2016-07-06" version="1">MVDDKLAVTIMIAMILAGGGKVLIQLVQHQQLKNGPEPERSEEILNNGPENDVVGEVVEVIDGDTINIIVTDVNGTKKGIWIGKEDTVRLAGGIDAPEFYENGYIKARDFLRSLLPQGSKIYLDLDDLAKSKTGRPYRDLTSSERLVAVVYVRVDNRWANINAEVLRWGQNVFPNRDWLAYTDIASEFNPYKWLEENHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPG4</accession>
    <name>A0A133VPG4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPG4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="77"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="391" mass="43887" checksum="2F484DA1379D74B5" modified="2016-07-06" version="1">MLSLSKDSVIFSDRENLEHKIGEIVERIKSLGRRRKLLASVLKKAKDGFRFQADEKHGGLIESALVKSVGRDSLSDCTVAGVDGGVLKKPLHGFDLILFRAVAAIFHYEYGSLRNADYCPSDMPPPQLMSIDEPLDSREFELLVAMRRQLAEMERARETVENWDVDAIFLDGSIVPQYVTPASESKTSETYGKLIDSFKGLYRTCDEKGALLIGVLKDSRSARFIKIFQRMVLPCLMNDSGLSASEVSLLESNKSVLSNSRDINFLDELLDTNERSFTFPYAGSQANLLNGLGEWKKRIYAFYLKSVPYDCPLRVEFLSGSKDIHETVDRISALVNALSAHHEACAFPPVLIEADACARLPMEEISIIRDNIADRLEPSSILDLRRDRRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPH0</accession>
    <name>A0A133VPH0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPH0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014942">
        <property type="entry name" value="AbiEii"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08843">
        <property type="entry name" value="AbiEii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="192" mass="21954" checksum="366941FEC1580751" modified="2016-07-06" version="1">MIGITGRVAEIFEEANCPYVVTGGVAVVYYGVLRLTHDVDFLIQKGLSESRINKLLNLLHAEGFSFDEKEVHQRLRQGGMVRMTGAEGFVKGFVVDLIARPRMDPILEHSRKVEEGKIHMISPEDLIVQKLLIIKETSPPKLRPHDKEDVVALLIAREELNLEMDYLHERAKEERVDNLLEKFLKKIEELAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPH8</accession>
    <name>A0A133VPH8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPH8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="79" mass="9559" checksum="ED4FD71E09928BFE" modified="2016-07-06" version="1">MFVTKVPIENKKNVEIKCREIREVDETFRFNFKEKSYGWLLIVSSRTKDQAFQRGEWLINKCDPIKAQDTYYWVHEVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPI6</accession>
    <name>A0A133VPI6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPI6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="315" mass="33415" checksum="094944A42B14DC1F" modified="2016-07-06" version="1">MKLNLGKEVSILGKSIPVAILILIAITGIASAGLLAHYVTVSGTADVKQSVVFSDETTSKTFSFDGTDVTAGDTRTDIYTLETRAEKPAKVGFSTNQDDLSTSGNEGDVTGIDTSYYFVTTVFAKGSGSATWKDDGTVDISVASDGSGAGVALVPVDRAISDKGDLDSLISQMSVTGTGDIYKQLMIDLDPSDASSNWAQATPNGFFVYSSEGEFVETYSDYSNIDSSYLDKAVVVGAKGQIWRGDGASGGGTVDSITFDGNTYELNKDDRTMLQKSELTFPPLPKEEFDIAVVNDFAVDLEPHQYEITSNVNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPJ3</accession>
    <name>A0A133VPJ3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPJ3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="260" mass="30445" checksum="6A2CA661FEE307B9" modified="2016-07-06" version="1">MFEKLTEMKEGIGRKLNNERVLEKLDTVEKELKEMEERQKKLSEIDRKLDKIKKGEGNKTLKTIKEDLKELKKEGISGKETAEKLSLIVDLVRDLKNEQLGYGEIAKQLREIQEFVLLDDLAEIKENKEGECPFGKHEEFKEKLDNSPLLCKKTLVMATLIQKRKSITTQEFAELTKYSRQHSYWILEQFVDFGVLYKEKEGRGQINHYKPHRNSVGEPLLRDFLPYVRKRLTKAGICNYSIMDIRDIKRIGKIENLGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPJ5</accession>
    <name>A0A133VPJ5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH deoR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPJ5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006793">
        <property type="entry name" value="FaeA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04703">
        <property type="entry name" value="FaeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9939" checksum="C607FB2297E6DB76" modified="2016-07-06" version="1">MGTFTKEVKYNKENWKIDEKEIMEFARHQEVVKGQDVAKEFDISYETARKYLDVLTHEGKLKRNELESWEGNKKYYIYRVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPK1</accession>
    <name>A0A133VPK1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPK1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018716">
        <property type="entry name" value="DUF2240"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09999">
        <property type="entry name" value="DUF2240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor A C-terminal" evidence="6">
        <location>
            <begin position="543"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="687" mass="76393" checksum="99170E45F63B7F4E" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPK5</accession>
    <name>A0A133VPK5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="5">PGP</shortName>
            <shortName evidence="5">PGPase</shortName>
            <ecNumber evidence="5 6">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="5 6"/>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPK5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07514">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002245">
        <property type="entry name" value="PRK01158.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="229" mass="24635" checksum="A1211B1A38C14BCC" modified="2016-07-06" version="1">MIEAIAIDIDGTITHSNRQLDIAAVDPIRRAEKMGLRVIVATGNVLCFAEATSTLLGTTGPLIAEDGGVVYDKSEDQEYVIGNTEKVDEGVKLLEERFEHLQHTRTSKTRHAGRTLERTFDVNKALEVLREAGLDLQVVDSGFAIHIKDPEISKGNALKIVASLLGISTSEIAAIGDAPNDVEMLRNAGLSFAPSNSHPKAKAACSYALDAPHGKGVKKAITKILKERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPK8</accession>
    <name>A0A133VPK8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="7">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxS</name>
        <name evidence="10" type="ORF">AKJ58_00030</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPK8"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.7"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="7">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KXB08379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="302" mass="33149" checksum="AEF04F93CA3A0BEF" modified="2016-07-06" version="1">MQRKTEDLVAEYGTERLKRGFPRLQKGGVIMDVTTPEEARIAEESGAVAVMALQAVPADIRKAGGVARMADPSIIEEIMDATTIPVMAKCRIGHFAEGQILESLGVDMIDESEVLTPADKEHHIDKHKFKIPFVCGSRDLGEALRRIDEGAAMIRTKGEAGTGNIVEAVKHIRQTLRQIKKLRNLSTEERKKKAEEYMVPPDLVEEVAELQRLPVVWFCAGGIATPADAALAMQFGVDGVFVGSGIFGSEDPSRMARSIVEATRYYDDPKKILKASKKLPQAMKGIDIESLAPEERLQTRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPL0</accession>
    <name>A0A133VPL0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 14" type="primary">radA</name>
        <name evidence="14" type="ORF">AKJ58_00060</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9 11">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 11">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPL0"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.13"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002052">
        <property type="entry name" value="DNA repair protein RAD51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00348">
        <property type="entry name" value="RadA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011938">
        <property type="entry name" value="DNA_recomb/repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02236">
        <property type="entry name" value="recomb_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="12">
        <location>
            <begin position="89"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="13">
        <location>
            <begin position="265"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="118"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KXB08385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="324" mass="35493" checksum="63E77E0B15D37F59" modified="2016-07-06" version="1">MKFYLLRESLNEVENSVENLPGIGKATAQRLQDSGYQSIESLAIAKVQDICEAAEVSESKAEQIVEEAKKSSKIGEFKTAEEILKERGNVGWIQTGSDALDNLLGGGIETQSVTEIFGEFGSGKTQLAHQLSVNVQLPKDKGGLNGKAIYMDTENTFRPHRILSMAEGLDLDSEETLKSIFVAKPLNTDHQLLLAEKAQKIVKDEGVRLLVVDTLTSLFRAEYVGREVLAERQQKLGRHLLTLHRIADLNNVSVVVANQVHAKPDAFLGDPTQPIGGHVLGHSSTNRIYLRKSKGTRRIARVVDSPELAEGEAVFSIATEGIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPL5</accession>
    <name>A0A133VPL5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPL5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="76" mass="8355" checksum="D9A4851C3711B590" modified="2016-07-06" version="1">MVRGHILITTAPGEMRDIRERIESLSNIKSVEMVTGPYDLIAQAEAEDLSTLTSTVLDNIRHITGVLDTTTCVVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPL9</accession>
    <name>A0A133VPL9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="3">HMG-CoA reductase</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_00045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPL9"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.10"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00644">
        <property type="entry name" value="HMG-CoA_reductase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004553">
        <property type="entry name" value="HMG_CoA_Rdtase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00532">
        <property type="entry name" value="HMG_CoA_R_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="420" mass="45259" checksum="922226BCA51E259A" modified="2016-07-06" version="1">MSKTSDISGFYKLDRKERLKIVKKFAELSDKDVTTLKKTGALDYEQANRMIENVIGTMELPLGIAVNFLINGKDYLIPMAIEESSVVAAASNGAKIARVRGGFEAKSTRPEMIGQIQLTGLKNIERAKRNIMEKKDEILELANEQDPVIVELGGGVKDLKIREIETPRETMIIIHLIIDVRDAMGANVVNTMAEAVAPLIEKITGGGVYLRIVSNLATHRLARAKAVFDKEAVGGEEVVDGILEAYAFAASDPYRCATHNKGVMNGVVALALATGNDTRALESGAHSYSALGGNYRPLTSWTKDETGNLIGEIELPVAAGILGGATKLNPVADVCLKILGVKSARELGEVMASLGLAQNLAALRALASEGIQRGHMRLHARNIASLAGAKGELIDEVAKRLIDENQINVKRAEEILREMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPQ8</accession>
    <name>A0A133VPQ8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein AKJ58_00095</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPQ8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652:SF1">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="127" mass="14269" checksum="788ADF1E47947641" modified="2016-07-06" version="1">MRIEIRANVHPTENPDKIKDAIRKIFPDMNIALKEDYVMGESSSLDSLEQFKNKLRLQAIRNSARRVMLESRKNNSLSFMLNRQVATVGKISFSDGKTALGPVIVTIESEDLDRVVDHLAPSTSGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VMY3</accession>
    <name>A0A133VMY3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_01710</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000034">
        <property type="protein sequence ID" value="KXB07806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VMY3"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.278"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="133"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB07806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="166" mass="17904" checksum="C50C8DA7C3FD5F8F" modified="2016-07-06" version="1" fragment="single">MPKREDIEKVLLIGSGPIEIGQAAEFDYSGSQACAALREVGVKVVLVNSNPATIMTDPEMADAVYVEPLTAETVAEIIKKERPDGIVAGLGGQTALNVTSELAEMGVLEEYDVEVLGTPIEAIQNTENRDKFKKKMEEIGEKVPRSKAVCSLEEAKEVVEELGFPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VMY8</accession>
    <name>A0A133VMY8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATPase AAA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AKJ58_01725</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000035">
        <property type="protein sequence ID" value="KXB07799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VMY8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000038">
        <property type="entry name" value="spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48" evidence="5">
        <location>
            <begin position="50"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="184"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="457"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB07799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="77364" checksum="648FCD4005589F42" modified="2016-07-06" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN20</accession>
    <name>A0A133VN20_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000031">
        <property type="protein sequence ID" value="KXB07829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN20"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="88" mass="10376" checksum="AE8D4CE743D0E8CD" modified="2016-07-06" version="1">MATWECIKKKFKRIQSIIFGKKEDEGEGTRKLPAMKAPSHYVSKTASQRFPNESIDEFIARAYSKFEGPSIHPPRRKERRKIRRRRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN23</accession>
    <name>A0A133VN23_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Resolvase HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000032">
        <property type="protein sequence ID" value="KXB07817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN23"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase HTH" evidence="2">
        <location>
            <begin position="3"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB07817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="190" mass="21805" checksum="89BC1B437C73BA6C" modified="2016-07-06" version="1">MPRRKVSEEDIERMKSLKNEGLTYEEIAEEFDVSYSTVAKYLRGVGKKTKITPEILEKMKNLRESGLTYEEIAKELDFCYSTVAKYMKKEGLGGRRKKVTSKMMVLPMLSFKCGGYFRTPHPRIPLRSVSNPIMLALKEVPLKALRDVGRARKVLQSTSMEANGRREPHKSVRPARNSSVNLGSWGRYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN31</accession>
    <name>A0A133VN31_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000031">
        <property type="protein sequence ID" value="KXB07830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN31"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="144" mass="16775" checksum="8D07FCA606ADC6DF" modified="2016-07-06" version="1">MNDHLKARRLKTLQYHLLNVDNWAKKISDEFGCSVSAAREDYRRRSEWLSDLDHQYLDSTSDLVAELLLEKSLVRSKAWKMYLKTKNDNCKLGALKLVDRSVNKEISILQSLGELKKKIGELEVSPEDRFTMNILPYEEENGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN34</accession>
    <name>A0A133VN34_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PD(D/E)XK endonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000028">
        <property type="protein sequence ID" value="KXB07862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN34"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17805" checksum="1177AF563C26D1F1" modified="2016-07-06" version="1">MSDKELTGLAGEFEVASELCRRGFYAQLTLKSRERTDVIVTRDEGFIRVQVKSKVGSCWPGCKGVYGKDSLLFFVDFEGKDLTERPDFYLLKPGEWRSLLEEALEDKVESGKVKIDEKNIPIWPDQEFKGYDVHPEEIKEHKERWDKVEESLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN43</accession>
    <name>A0A133VN43_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000026">
        <property type="protein sequence ID" value="KXB07885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN43"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="3"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="67" mass="7820" checksum="B34FD0812D34C978" modified="2016-07-06" version="1" fragment="single">DKEIYGADISDTALNYCRERGISNVYDLEKTEPPKDHFDLITCLDVLEHVHEDVDPSERRSSPMQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN44</accession>
    <name>A0A133VN44_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000031">
        <property type="protein sequence ID" value="KXB07831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN44"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="153" mass="18111" checksum="4D6F0DFDF989F3A7" modified="2016-07-06" version="1">MFYILECQECALKRVEEADADSDSSEMILFEMRQQVSDHIRKTGHTKISIKKLGSNKVPQKERIRRAEEERKQRVRERKERKQKSKEMEKQWAREQYQELKKSGNERIQAALRMKRFDLLPAVTRKNLEPIIEELKAKKGVVPGNPKGYGVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN45</accession>
    <name>A0A133VN45_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_01635</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000029">
        <property type="protein sequence ID" value="KXB07850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN45"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="3">
        <location>
            <begin position="3"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB07850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="389" mass="43339" checksum="CD5C3B12D121CF82" modified="2016-07-06" version="1" fragment="single">MDDETLRFVIVGHVDHGKSTLIGRLFYDTDSLPGEKYDEIKEACEAMGKDFEFGYVMDHLKEERTQGITIDTAQTFFDTDRRRYNIIDAPGHVEFVKNMITGASQAEAAILIDDVAEGVQEQTRRHAYILSMLGLDQVIVVLNKMDLVNYDQGKFGEVKEKLLDFLDSIDVKPSHVIPISAKEGDFVANRTKELSWYNGPTVLEALDTFEKKRPPIEKPLRLPVQDVYKFDGKRAAVGKVESGILKEGDPIVVLPKGGETTVGSLEEFGKNPKKAIAGKSIGVATADKIFIDRGDVIVSPKKLPTVKDEFEGHIFWLGEEPFEEGERITFKCATQEVMSDIKINEVLDSSTLEIVSENGGKIENRQIANVTIKTSKPVVVENFNDVQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN46</accession>
    <name>A0A133VN46_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome assembly chaperone family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000027">
        <property type="protein sequence ID" value="KXB07872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN46"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="30393" checksum="5EE045BF97FAA995" modified="2016-07-06" version="1">MGFRIEDIELDYKVNKEELKAEVGEPIFIEGLLGVGHVGLLASTHLVKELRCKKIADVYSPHFNHPASHADIPGVVYSEDGMVTLNRNEIFYNKENNLFIYKGIYQADYCEFYYRHANKIMDFCEEFNVSGVYTLGGLGTGEEKENSEARAVITDPKTAEIVQNHVKILKGREDEPGVTGLSGLLIGLAHKNGITGISLLGETQGRFPDPKAAKSVLKALSGIIGFEVNLESLEKVTKKLEKKKKKIRKMLEWRRARRKEEEPEDLHYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN61</accession>
    <name>A0A133VN61_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Periplasmic copper-binding protein NosD beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000024">
        <property type="protein sequence ID" value="KXB07905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN61"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26463" checksum="B1F331674CB6A3CB" modified="2016-07-06" version="1" fragment="single">NNIVQYNNIYNNTNNGIQHNGTGTLDATYNWWGDSSGPSGVGPGEGDAVSENVKYNPWLLNPFEEPQTYYDHCEALNSGWNLISTPKILENDDISEMVADMDNLQHVYRYENSGWIEDNAAYGQEPLYASFVKVKEEAGSIGIGYNWSTDPQDVPPSRDLPEGWSMIGPSIDPSVEPSVPADEFLDSVSESYSTLFSPDFNLNSWTVTTKTAADENVLPFEGYWIFMESLDTLAGRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN62</accession>
    <name>A0A133VN62_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TDP-4-oxo-6-deoxy-D-glucose aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000026">
        <property type="protein sequence ID" value="KXB07884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN62"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.216"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019180">
        <property type="term" value="F:dTDP-4-amino-4,6-dideoxygalactose transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000037">
        <property type="entry name" value="dTDP-4-amino-4,6-dideoxygalactose transaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012749">
        <property type="entry name" value="WecE-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02379">
        <property type="entry name" value="ECA_wecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008687">
        <property type="entry name" value="PRK11706.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244:SF34">
        <property type="entry name" value="DTDP-4-AMINO-4,6-DIDEOXYGALACTOSE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="375" mass="42397" checksum="682D153BE8DA7275" modified="2016-07-06" version="1">MKIPLNNPYLTGNELKYMKKSMESGELVGGGEYTHKCEDLLEREFGAKKVLLTSSCTHALEMASLLVGLGSGDEVIVPSYTFVSTVNAFVLQGAEPVFVDMREDTLNMDESKVEEKISKNTKAIFPMHYSGVACAMDEIIDIADRHGLRVVEDAAQGVNAKYRDEYLGTIGDLGTYSFHGTKNYTCGEGGALVINEDGFIERAEIVREKGTNRSKFLRGEIDKYTWVDIGSSYLASELTAAFLYAQLEELEKIKKKREKTFGFYHDNLKDLEEEGKLRRPVVPEDCEVNYYSYFVLLPSEKKRNSVMEKLKNSGIQATFHYIPLHTSPMGAKFGYKKGDLPTTEKVSNRLLRLPFYTEIEKEKQKKVIEELERCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN67</accession>
    <name>A0A133VN67_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF885 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000027">
        <property type="protein sequence ID" value="KXB07870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN67"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.219"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010281">
        <property type="entry name" value="DUF885"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33361:SF15">
        <property type="entry name" value="DUF885 FAMILY LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33361">
        <property type="entry name" value="GLR0591 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05960">
        <property type="entry name" value="DUF885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="65206" checksum="47DB8381E15138B5" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN71</accession>
    <name>A0A133VN71_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000028">
        <property type="protein sequence ID" value="KXB07861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN71"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="250" mass="29798" checksum="102211FA73792563" modified="2016-07-06" version="1">MLCLTLFEKTTSIVNLYLLSHKYFLMPISKDFHDYFSEYSYRMDDPEYRRIYEKMWDDGYKLGEYGKALENFESLSTEKLFSILLDIGKWKAGERWYKKGYDEYWNHETIKRVLDVITSENSDRERIKAFGWGIKGFNKRTGSAVLTVVYPDQYGILDYKVWRALNHRWSREYDLDIECEQKNKGKCPGCYESNRCRYKLGASNSKPFNLAECDQYFDGIRTIGRGENMNPRQVDMALWEYDSQKSSCFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN72</accession>
    <name>A0A133VN72_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF35 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000023">
        <property type="protein sequence ID" value="KXB07915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN72"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="3"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB07915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11569" checksum="1F60B5537F3FF92F" modified="2016-07-06" version="1">MEGIKCRGCGRLYVPPMMTCISCGSNEFDRVEFEGKGNIVTYTVIHVPTREYKDEAPYTVAIIELEEGAKITGRVKGDPEKLAIGKSVKLLSEGKYYLFELVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN80</accession>
    <name>A0A133VN80_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000027">
        <property type="protein sequence ID" value="KXB07871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN80"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="65" mass="7543" checksum="67D9487D3894148A" modified="2016-07-06" version="1">MFKTTQISSYRMTLPEHYYVEMPLVKDLIKILHQAIPPVNNKYFLKAPEKLHKSGGRSVIKNLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VN98</accession>
    <name>A0A133VN98_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-CoA acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_01470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000023">
        <property type="protein sequence ID" value="KXB07916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VN98"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.195"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="247"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB07916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="41910" checksum="FF06D9E6B119DEEC" modified="2016-07-06" version="1">MRDVSIIGVGETKFGALDESLRELIAEAGNKALKDSGADRGEIESLYFGNYCGDRFVKQGHSSSLAALATGLKYIPTIRTETACSSSSGAFKEAVLGVASGAYDVVMVLGAEKMTNVDTLEATDILAMAGDREFEGIQGLTFPGMFALMAQVYSKKYGIKMEDLRKQLSSVAVKNHRNGYYNPSAHMRKIITLEEAMDSRMIAYPLNLFDCSLISDGAAAAILCPSNMATDYSDDPVDVIGFGHSTDILPLHLREDLSRSKATIIASEKAYKMAGIKPEDIDFTEVHDCFTINELISLEGLGFFDSGRAGEASLNEKTTVDGSIPVNPSGGLKSKGHPVGATGAGQIVESVMQLRGVVEKERRVDDAEICLVHNIGGSAVTCFVHILRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNA2</accession>
    <name>A0A133VNA2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000022">
        <property type="protein sequence ID" value="KXB07928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNA2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="72" mass="8243" checksum="67A402C47744DB34" modified="2016-07-06" version="1">MNNTKQYKEPRNIVDKDRYLRNNRTNESGVSVFYLGGNNYNIIATSKSNDSQDEKIISLKENSVTQVTLDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNB5</accession>
    <name>A0A133VNB5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000024">
        <property type="protein sequence ID" value="KXB07903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNB5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="81" mass="8869" checksum="C20A72E5657B28AB" modified="2016-07-06" version="1">MMRQSRTEYVVPAVVVIVLISLLFMGLWAIDISLSAAMMGARLTNGFITRNPIQMLHMGYYAVIGASVGLATLTVAILLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNC7</accession>
    <name>A0A133VNC7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000021">
        <property type="protein sequence ID" value="KXB07946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNC7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="332" mass="39403" checksum="4903B4936E44761E" modified="2016-07-06" version="1">MARKQEEKILSLLRSGEKRVKDLVALSDMSERSTKRWLKKLRNKGKIKRRPGEGREVWYYIPKWEGRPKPPVRKAPHDLVNECLDDLESSLIKEKGEVKRQIEEKAQEKDEHAKRVLWIIERYKNSWETITDLRIFLNEIKEKPREPGFWDKPDIIELARRELQSSAVKKFSDLCNQYKKIIYNDEIRRRFFELLDRQNDFYPGDFDVLLSTLKILTGGAMKGNEDPRIVLGARKRIGLLEDLCLREEDFTLSSGTLRTIRELDEDRAREIFVKMVVDEKYPMELLVDWALTYYRDELGDLIEELKKLKKPSSKAKERAREFRNMLEKTVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNC8</accession>
    <name>A0A133VNC8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AKJ58_01370</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000021">
        <property type="protein sequence ID" value="KXB07944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNC8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001623">
        <property type="entry name" value="PRK00411.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF31">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="53"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="7">
        <location>
            <begin position="304"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="65"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB07944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="397" mass="45567" checksum="3D6B73D7CF43C16F" modified="2016-07-06" version="1">MFEDIFEEEMARKSIFKDQSKLSPDYVPGTLVHRDEEFRELSRLFKPVLEDKASQRVLITGSVGVGKTALSLRFGRELEAGAEKRGFRHDYVHINCRKERTPYTVIRELMNHYFPRLSRRGFAPEELLSDVVDWLESHDAYLTVGLDELDFFVRQNGPDLLYSFIRAGEETGAPNRLSVIAISRSSNFFRLLDEATMSTFMHNKIELDKYNAQQLTDILKNRVKVAFRPGTVNDEVIDLISEAASQRGDARFALELLWHAGRLADKEGLEKVTLENARTAKARIHPEIRTDVLLDLRRHERLLLLALARHLKISDKAYTFTGELDEAYKVVCEEYGEKAFKHTKVWEILKKLSDFGLIKTQPAGMEKGGRSQRISVPDAPVETLEKELEKILDSKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VND4</accession>
    <name>A0A133VND4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000019">
        <property type="protein sequence ID" value="KXB07975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VND4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="112" mass="12473" checksum="76B1962F09186A22" modified="2016-07-06" version="1">MAYAFVPGVRAKINGLFSNSDSEPILSPSEVREHPNQYLGEKITIEGYYLPVWGICEKTLGNDPYSEIVYVKNASNFNLIGGGEYRFTGVLEQSETYVWCGVCLNLLEVEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VND9</accession>
    <name>A0A133VND9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000019">
        <property type="protein sequence ID" value="KXB07972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VND9"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="81" mass="9687" checksum="619C4B14B54B4908" modified="2016-07-06" version="1">MSWKSFEERKKKALAREEKDEWSQRKKEAVLSAAEKYAKEFDLDPDELLRSFISWDSFRNPIQPEEVDEYMGERAEGLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNE1</accession>
    <name>A0A133VNE1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable L-aspartate decarboxylase</fullName>
            <shortName evidence="6">ADC</shortName>
            <ecNumber evidence="6">4.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mfnA</name>
        <name evidence="8" type="ORF">AKJ58_01350</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the decarboxylation of L-aspartate to produce beta-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-aspartate + H(+) = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the group II decarboxylase family. MfnA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="6"/>
    <dbReference type="EMBL" id="LHYK01000020">
        <property type="protein sequence ID" value="KXB07959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNE1"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.152"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01610">
        <property type="entry name" value="MfnA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020931">
        <property type="entry name" value="MfnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03812">
        <property type="entry name" value="tyr_de_CO2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6 7">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB07959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="371" mass="40995" checksum="D187F01730BF94CE" modified="2016-07-06" version="1">MDEKGVKEEKVASILEEARSKDVPYQRVLSSMCTRPHPLAIEAHRMFIETNLGDKVIFPGTAELEEKAVRMMGEMVNFSEVSGYITTGGTESNVQALRAARNMSDIQYPNVIVPESAHFSFDKIADVLRIEVRKAPLDDEFRADVGEVENMVDEKTLAIVGIAGTTEHGQIDSIPALGKIALDNGIFFHVDAAFGGFVIPFLEKKYEFGFEIKGVDSITMDPHKMGLSTIPAGGLLFRDKSFIEALKVHTPYLTTKDQYSFVGTRSGAAVASAFAVMKSLGREGYRKIVRRCIDLTRGLMDELGDIGVQPLIRPPMNVVTLDVAGERVKERLYDKGWITSVTRGGALRLVIMPHVTEEVLDDFIHDLKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNF0</accession>
    <name>A0A133VNF0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000021">
        <property type="protein sequence ID" value="KXB07947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNF0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8566" checksum="4B2287D5FA5F9DAA" modified="2016-07-06" version="1">MPQVPIIETRRIHPIGEGGLSILLPKKWADRKGLQAGNDVLVLADDSVTVEPVTSERIEEIERAIDRVIKRGDPNGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNF5</accession>
    <name>A0A133VNF5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000021">
        <property type="protein sequence ID" value="KXB07950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNF5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9757" checksum="D79CDB7A249E9948" modified="2016-07-06" version="1" fragment="single">MTRSARDDAPTDDEFGRMLEACKRTDHPLESRFLLQTLGPPGGMRVAEVLHMKEGWIDWNEGIIKIPGHEPCDCSYCQERARKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNF7</accession>
    <name>A0A133VNF7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrapyrrole biosynthesis glutamyl-tRNA reductase dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000019">
        <property type="protein sequence ID" value="KXB07976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNF7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033014">
        <property type="term" value="P:tetrapyrrole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="Glutamyl tRNA-reductase dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis glutamyl-tRNA reductase dimerisation" evidence="1">
        <location>
            <begin position="59"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB07976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="119" mass="14116" checksum="6271B68D2C01C01D" modified="2016-07-06" version="1">MPTNVHPKVRENKMIEYISYEELSKKARENLINRSGEVEKIRKMAREEVENFQEEDPYEDLYKEVEVIRREQVEKAKKELGKREETKALQDFSRSLTKKILSVVKKEINKNERSSGASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNF9</accession>
    <name>A0A133VNF9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNF9"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="60" mass="7135" checksum="8920D8D1D4842EFB" modified="2016-07-06" version="1">MNYEDLDVDMSTVYGWVWKLAFRELTKEGRLELAHDLIKCSLFDQEHRYKVLESLEEAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNG6</accession>
    <name>A0A133VNG6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_01355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000020">
        <property type="protein sequence ID" value="KXB07960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNG6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048515">
        <property type="entry name" value="DHH_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051673">
        <property type="entry name" value="SSDNA_exonuclease_RecJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255:SF3">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21763">
        <property type="entry name" value="DHH_CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="DHH-CID" evidence="3">
        <location>
            <begin position="189"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="379"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB07960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="464" mass="51213" checksum="C2002BB70142DD5B" modified="2016-07-06" version="1">MEGTAKEAAEIIERHKNDSITLISHMDSDGISAAALLSKALDRLEIQHQTKFVRMLYPEVVEDLEVGNLTIFTDLGSSQLSNLRGKFEGSDTIIADHHEPEETEGWPRLIHLNAHINGFDGAEEISGAGMAYLVAQKLNSQNKDLAALAIVGAIGDIQNAWGGLQGINRRIAQDGIDAGTIGQEKDLLLYGRHTQPIFRALEHLTDPPIHDVSNSTEGCISMLKDLKIPYKTKNGYRRPIDLEEEEKRRLASELITRAMINVPDELVEYVPGLIIGEVYTLLNEREGSLLRGADEFSTCINSTARHEQPLIGLEVAKGNRDVYYRAMRKLLKYHKRCIAEGMSYIEGEGLKKGPKDKVQYFDATDVVKETFIGTITNLALGHEKSDPYKPIIGLVQRNGTTKVSARCSKLLFLKGINMARAINDAANSVGGEGGGHAVAAGAQIDTEKIPKFIEEFENRLFEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNH6</accession>
    <name>A0A133VNH6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M10 metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_01270</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNH6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M10 metallopeptidase" evidence="6">
        <location>
            <begin position="225"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB07994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="305" mass="33804" checksum="F237AC03E8DD1243" modified="2016-07-06" version="1">MGAEAESKGDVGKRKRRVGLWEVEAQNLKRPAEKGRNQIFCQGSSSKLSRSCLVFKYLRIQILYMGVKMKRLITGLVFAALFLGTLSAVAAGGGPSRAPELEKVTFVHYEKSSQSGPPTWDDTEDDYRLIMGGAKWLETMTYELNPGTVPGYLLALDLINTKMVLEESMETWDKEFGFNLFADSIGSTVVSSVGNDGTNLVMWSDLDDGVIAVNTLWIDPASKEIVESDVQFNTDYTWSTAEDCPKEEMDLQNIATHEFGHNGLDDLRPLKDGDLTMYAYSATGETKKRTLGYGDELGIQDLYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNH7</accession>
    <name>A0A133VNH7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="5">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AKJ58_01220</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="5"/>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNH7"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.116"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="HisA/PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010112">
        <property type="entry name" value="PRK13585.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF7">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KXB08015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="217" mass="22760" checksum="2553C5CC2C13279C" modified="2016-07-06" version="1" fragment="single">GKPGTEREFGDPVDAGIKWVTEGANYLHLVDLDAAMGEGDNLKKVAEVLASVGVGVEVGGGIRSVDRGCELLGIGADRVILGTAALKDPDIVYELAKIAGPDRVVVALDARGGKVAYEGWKKTSERGVIEVAKEFENLGIGGILFTNVDVEGHMKGIEPESIRELVEAIDLPAIAAGGVRSRRDVVKAKETGADALVIGRALYEGKITLKEAMEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNI4</accession>
    <name>A0A133VNI4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">molybdopterin molybdotransferase</fullName>
            <ecNumber evidence="4">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AKJ58_01175</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoeA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="4"/>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNI4"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.106"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000004">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.340.10:FF:000005">
        <property type="entry name" value="Molybdopterin molybdenumtransferase MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="11">
        <location>
            <begin position="186"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KXB08008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="413" mass="43857" checksum="54C687F4B5795E42" modified="2016-07-06" version="1">MHEPLRVVTIQEARSKLEEHWQPQRRVKLFPLDEAGGMVLAEEVSSEIDLPPFDRSDYDGFAVRAADTFGAGEDDPVELFKVGEVLAGQDPEVELERGQCATIATGAQVPNGADAVVMVEDTTSIGERVEVRRSVTPGENVSERGSEIEQGAKIVSAGRRLSPQVHGTLFACGVRDVKIFCKPKVAVISTGQELIEAGSKLGPGQIYDVNGPAICDAVNNCGAEPSYLGIVQDSFSEIEERVREALSDHDLTIISGGSSAGAKDIVPETVNGLGDPGVLVHGLAQKPGKPTFLAVIDGNPIFGLPGYPVSALMVFDQLVAPYLRFLSAAPEVGRKRIRARLAKKVLSARGRRELVPVSLDEGDCLLAHPLRKGSGAITSLANADGYIAISVDQEIVEEGAEVTVNMFGGSELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNJ0</accession>
    <name>A0A133VNJ0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNJ0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="153" mass="17627" checksum="BCFA48774AD322B5" modified="2016-07-06" version="1">MRIDQIQKDKIKHTLKNAGEMLGFSVITDRDFWTDGKVEIEWAGKLCSMRGIRYGFKTVDLDEPSEKMEKTVKGCQEMNYAGLILIAKEENLKEVKKLYDSSLVVSYMSMEEVSKVMDLLVDLETIFHKKLITRSDGRSPKEAQKELGQMIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNJ7</accession>
    <name>A0A133VNJ7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sugar fermentation stimulation protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sfsA</name>
        <name evidence="4" type="ORF">AKJ58_01125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SfsA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNJ7"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.96"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22359">
        <property type="entry name" value="SfsA-like_bacterial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00095">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005224">
        <property type="entry name" value="SfsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040452">
        <property type="entry name" value="SfsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041465">
        <property type="entry name" value="SfsA_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00230">
        <property type="entry name" value="sfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545:SF2">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03749">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17746">
        <property type="entry name" value="SfsA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SfsA N-terminal OB" evidence="3">
        <location>
            <begin position="18"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar fermentation stimulation protein C-terminal" evidence="2">
        <location>
            <begin position="88"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="238" mass="27206" checksum="946F6CA45FE1E336" modified="2016-07-06" version="1">MKKLILTFDGGLKEGNVVSRPNQFTLIVDFSDSTERVYLANPGALTTVLSPGNKILCKPVKSKNRKTDFDAFAIEMGDIYAIVNSTFADSIFSNILKRGLMEEFKKYSILAREPPLPDRGRADFLLENEIGNKTYIEVKSCTHVENEVAKFPDRPTERGRRHLRSLMVLKKNGFKSYVIFVVQRPDVKKFQAYRRVDPEFADLLKKSEEEGVEIRALSTEFRPPDLFLKNKDLPVELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNJ8</accession>
    <name>A0A133VNJ8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_01180</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000017">
        <property type="protein sequence ID" value="KXB08016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNJ8"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.108"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000004">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF19">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN MJ0666-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="178"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="400" mass="42117" checksum="9CCB85D18EC8DE72" modified="2016-07-06" version="1">MRLEHALSIILSNVHGLGVEEVPFDSSFGRVLADDVVSKVDVPPFDRSAMDGYAVRASDTFGAEEDDPAELRVVGSADIGTPTEVKVGDGESVEIMTGAPVPEGADAVVMIEHTRLENGTLKVFNPVSPGENVSSRGEDVERGQVVLEGGHRIRPQDVGMLASTGTCLVKVTRRPKVGIYSTGSELREPGEELKEGEITLVNGYSLKAAVEEVGGKPFLLGIVPDEADSLKNAIGRASDFDVLLLSGGSSVGKLDMVPDMISDLGELLIHGIAIRPGGPTAFGIVGGTPVFALAGFPVASLVAFEMLVRPALRSIQGLPADRGRTTIKAILGRKISSSLGRVDVVRVEVKGEDEGPMAYPIRVTGSSVLSSMTRADGFIVVPEDVEGFSRESEVEVELYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNJ9</accession>
    <name>A0A133VNJ9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000016">
        <property type="protein sequence ID" value="KXB08032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNJ9"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008304">
        <property type="entry name" value="UCP017998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06557">
        <property type="entry name" value="DUF1122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="81" mass="9285" checksum="47F5E78A6E646BB1" modified="2016-07-06" version="1">MVIYEGHKETERGLQRNIPPPATPIGNLLFQSGCIWFKDWYFTGGYKEGSVKLQGEKTANKEARRKALVEIHQELSDFLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNK0</accession>
    <name>A0A133VNK0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000018">
        <property type="protein sequence ID" value="KXB07993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNK0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB07993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="61" mass="7121" checksum="01CA29C9181E873A" modified="2016-07-06" version="1">MSFEDLESDEESAIHDLAWRLVFRELSNEEMVQLAYEIVLCSMMDRNCRNRALKALKDVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNK4</accession>
    <name>A0A133VNK4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AKJ58_01075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000015">
        <property type="protein sequence ID" value="KXB08045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNK4"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.84"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015930">
        <property type="term" value="F:glutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate synthase" evidence="3">
        <location>
            <begin position="285"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KXB08045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="536" mass="59273" checksum="84266BD5555B1B7D" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNL0</accession>
    <name>A0A133VNL0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NIF system FeS cluster assembly NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000015">
        <property type="protein sequence ID" value="KXB08042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNL0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="141" mass="15667" checksum="6267ACD09C998486" modified="2016-07-06" version="1">MTEKKKKGKKYTERGFLDYSDKVIELFKNPKNVGEIEDADVVVQEGDPTCGDAIQLSLKFDNETIKDAKFLSFGCAANIATGSTLMEMIKGKTVEEAREITIRQVLEEVGGLPPVKRHCAVLATRALEKALEKALEEYGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNM7</accession>
    <name>A0A133VNM7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DksA C4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000015">
        <property type="protein sequence ID" value="KXB08046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNM7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9458" checksum="957EBFD11DFE4CDF" modified="2016-07-06" version="1">MEEEVICAVPGCDNPLTEEQREEKIAVCLECEEAGMHLCENCGREIEPERIRDGVSLCKECEMNPSALEGTDYTEYESENYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNN1</accession>
    <name>A0A133VNN1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_01025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000014">
        <property type="protein sequence ID" value="KXB08059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNN1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="180" mass="20000" checksum="244F9F6DE04F835E" modified="2016-07-06" version="1">MLTAIDIVRARSSAIESDLNGTLDTAITAAMREVGLGGGTRKNVEMRVRDYLNARIDRGWNYTSVNEDIARVDENNLRFEWRPDGSVTVHGLLPATIKHVNGPTAYGIRLYSASSPRFERLKYVAERVAKQAENENLNELERKLNENYAAEGLHITLTLSPDNAVEVRVEDTFGAKAIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNP2</accession>
    <name>A0A133VNP2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_01085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000015">
        <property type="protein sequence ID" value="KXB08047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNP2"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.86"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007380">
        <property type="entry name" value="DUF438"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966">
        <property type="entry name" value="BLL2471 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966:SF3">
        <property type="entry name" value="DUF438 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04282">
        <property type="entry name" value="DUF438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13596">
        <property type="entry name" value="PAS_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF438" evidence="2">
        <location>
            <begin position="14"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="91"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="438" mass="50575" checksum="AEFC5923A96809C0" modified="2016-07-06" version="1">MSNAKGREDKKKKLKGLLKRLQEGEEVGELKEEFKDLLKTVAPQEIPLIEQELIEEGVSTYEISKMCDIHVELFRESVQEKFDLEGIPSGHPLHTLYQENERITKDAELLVLRSSNLRRAGSEGERKKLLEELKDLASGLMGIDRKHYGRQEMIVFPHIERRGIQAVPRVLWRKHDENMGKVKQLLDLLSKEPANWGDFAQSVEEKAQDVSSSLVDMVFRENNILYPTLKELLSRDEWVAVSEQEKQIGYYKVQPGEEWNAEGEAKYPYEVEEGLSDQELGELPEQLKSMLRDQELKPDRYQLVKEEDKKLDTGFLNMDEINAVLNSLPVDITFIDSDNRVRFFSGGHRIFPRTRSVLGRPVKFCHPPGSVDKVKEILKEFKAGRREEAEFWIQAGGKFIHIRFFPVRGKDGKYLGTLEVTQDVTGIRELEGEKRILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNP3</accession>
    <name>A0A133VNP3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_01065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000015">
        <property type="protein sequence ID" value="KXB08043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNP3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="353" mass="40531" checksum="9D524DCBF5072551" modified="2016-07-06" version="1">MEWKSRGGPLAFRTMDDCVLSRGFKLKDLKGSNEKGVIEVSPCASERGKVMAEIELIEEDKPFLDMEILCLLLNSYKNHFAEMRCSTKLGVARLMWKARRIYIYEKGKFKVRFAHSRGDAVKTLNSVGRLILGSVLCKICGEPAVECALGKCDKCFSDKYPEVVQLKNNFNAPLLIRGVSSLEDAVEESQELINHLVSKKKWPDQIEGNMRRRLRDTIEFAMNFALETHDLEDLRIGTTLIAVARENLLILDLERKITEIKVESPKKFEKLMGKLERAVWRINKNVVERLFSKSHKKVEKADEKTPKALELLDEITGSEEYIHEEGVKNILEELKHYIGKNMRLLKKIDYVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNP8</accession>
    <name>A0A133VNP8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ParB-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNP8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045881">
        <property type="term" value="P:positive regulation of sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050336">
        <property type="entry name" value="Chromosome_partition/occlusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33375">
        <property type="entry name" value="CHROMOSOME-PARTITIONING PROTEIN PARB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33375:SF1">
        <property type="entry name" value="CHROMOSOME-PARTITIONING PROTEIN PARB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="63" mass="7027" checksum="3AC448EF9E6E3D56" modified="2016-07-06" version="1">MGDIEPLANSIDRVGLLNPITVRKDGSSYRLLAGFRRLEACKSLGWEKIPSQVLEEGESAWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNQ7</accession>
    <name>A0A133VNQ7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNQ7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="219" mass="25268" checksum="755B8B2BE260B257" modified="2016-07-06" version="1">MPKQKMGEDLEDLVSKAVRPDQRLKAEDLVASELATAILSEPLSKIRHTCEALMLLDESERKSANITEEEVKESEKIYTLTATLRNAFIDKFTDSYGNVIERSATIPWPFERKEVEEWLDWSNYPIWKIYVESGREKERVLKKARELHDEGKPLESLYHVAEYRVTIDTLNRLKVQFVNYAMPRTAKLLKKIISLVSSSSFQEALKRLKGGSYGSQRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNS8</accession>
    <name>A0A133VNS8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000013">
        <property type="protein sequence ID" value="KXB08073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNS8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="361" mass="41505" checksum="696DE57A764659C5" modified="2016-07-06" version="1">MDRRGKDKPEFEIFWTRGNRKIDFRKPNIIFSLGRRGSGKSAFLELMALKHLQAGGKILDLFGSKDGEGLAWLRSPLTKDKEIKLLHGNLVDLQGRDGIQATKFKLSDLDRGDIFISASPLYTSMDNEFDSVNHVFDTLWKRTYWDQPVFIIVREAANLLYSRIKIRPSQTMAKAELVYMLRESRHSGLALGLDSTKHTSIDIDIRKLTDYLVLKNLGLYGLPRDLWWIYSMFDPNALQNLKPNEFAIITKEGCMGLGSFDLPRWHKREGEHILKDVGVNPRYYEAEKESEQALEYVREAVETLTGKYTPTDVSEWIKNEHGVEIRPEGVGLYLRRIGYTTENRSIDGKQMRVVKQIGNSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNT3</accession>
    <name>A0A133VNT3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YbaK/aminoacyl-tRNA synthetase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNT3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411:SF1">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YbaK/aminoacyl-tRNA synthetase-associated" evidence="1">
        <location>
            <begin position="20"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="147" mass="16091" checksum="2BE09D48CF7CB29C" modified="2016-07-06" version="1">MKNFIEKKEIKVNFYEFSKPVLSVDSSAKELNVDPERIVKSLIFVDEEGTPLLVIVTGDKRVDEERLSEVHGQEVRLAKAREVEEFSGYKIGEVPPVGLVLKTFVDSKVMDFDTVIAGGGSTHALIKIGSADIVEVNGAKVADIGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNT6</accession>
    <name>A0A133VNT6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNT6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16179" checksum="C94920D4ADEE3890" modified="2016-07-06" version="1">MFVEENAKEKIWEFRTPLIPKINEDEVKYIGEFLSDIDEELPLNFLAFRPNFVLENHLGAKRAMMKRAVEAAKKAGLKNVSWSGHTDLSGYIAENKASEYGREGGKLAGGYAKGAGCVTHPRNCGSCKLQQQCPIKKYKAKRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNT7</accession>
    <name>A0A133VNT7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNT7"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.31"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF8">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YRBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="4"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="166"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="309" mass="33051" checksum="29A52AC7B83385C4" modified="2016-07-06" version="1">MWDSLLIIAVCFLIFERASNYLVEGLGAISHKFKISEAVMGASIAAIGSSAPEFGSSAFSVIEGHPTIGLGTIIGSAIFNVTVIVGAGAFFGKYAIERRVFYRDGLFYLFTVLIAIAFVWDGSLARFEATLWALVFFGYLAWLIFDARRGKPVPRTSLANLSPRRAFIYVIIGLLAIIIAARYLVIQVADISSSLGISEATFSLVIVAIGTSIPDLFTSVQGARMGMGSLAVSNALGSNIFDILACLGIPFSFSRKPTPIEGPLSISFVALLASVVLVLAVLRNDWSVSKIEAVLLFGVYGVYLGLILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNT8</accession>
    <name>A0A133VNT8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secondary thiamine-phosphate synthase enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNT8"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.46"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14532" checksum="43B04F8D46B1AD6A" modified="2016-07-06" version="1">MEEIRISSDSRNQMIDLTSRVQQVVHESGVEEGTCLIYCPHTTAAITINEGADPAVREDILNALDEIVPDIDFKHTESNSDAHIKSSIVGPGERVLVRGRKLKLGRWQKIFFCDFDGPRSRRVWVKVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNV3</accession>
    <name>A0A133VNV3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cobalamin-independent methionine synthase MetE C-terminal/archaeal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNV3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03310">
        <property type="entry name" value="CIMS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="37150" checksum="6B4E1B78038850E8" modified="2016-07-06" version="1">MRLIYDDVGSFPIPQGVEGKRFERAFLNSEEWALRVFNESMSMKIDAGVQVPCYPQLRDMNDQFLEPLKDPEIVEEPYLIREEKAVIPEIVALENMNMNGRKIKMCITGPLELSIAEFGNEVYQDVMMNIARSLNRFIKNAESIRDLDIGVVSIDEPSLGTNPNISVDEEMLIKAWDIAGATEKDVQIHLHSPLPVENACRSESIDVIDIGTAATPENLEMIDPEMLESYGKRLRAGISRSDISSLAAEFNEDRKVNVWTDKDAWEEFLTWVEPPRKISERIGKAFDRFGDLLKYVGPDCGLKGARSRSLAHIILKNSSKGIDLFQKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNV4</accession>
    <name>A0A133VNV4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AKJ58_00920</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily. NifB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000012">
        <property type="protein sequence ID" value="KXB08097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNV4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF13">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="38"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KXB08097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="269" mass="31027" checksum="C75F64058D0C0638" modified="2016-07-06" version="1">MEEYPRYLTPDSETFDPVELAERTRKIVCRGDERKYTRFYATGVYGGIATGYTCGCCLRCVFCWVDRSRDFPEKLGSFYSPREAFERLSEAARSYGTRKLRISGAEPTLCKDHLIGLLDRVEDSEFGPFILETNGIPFGVDEDYVEKISEFDKPHVRVSLKAGTPEAFSRKTGAKPQFFEIPFKAIRNLLKHGVSFHVAGMMDDPRIVTSRERSELARRLKEIHPKLLRYFEGEVVDPYESTIKRLKASGFSLSWPLKESYPPLWKEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNW4</accession>
    <name>A0A133VNW4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNW4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="36198" checksum="032BA4039DEBD44C" modified="2016-07-06" version="1">MRRVRIRDFLETEDMIFSVVTYKHPSNHYNALLRFVRDPRGERTRDNINYRKLSFQEAVNYINTWRPEYGDGEPHKVPDANLKRIVRPKKALSRLNKEGHAEITTITKKLLAEGANSEDLGITGSHLVGLDQPSSDIDFVAYGLKGFKAARLALRKGIKNGGIRSVEEETWGRIYRKRSPELDFDEFIEHEKRKFNRGMIGKRYFDLLFTRSWSEIKDIPSRGKKREKMVVEAKVTDDSFCFDSPAIYEVEHPEIDRVASYTHTYAGQVFKGEELQAKGWVEESEKETLLVVGTSRKAKGEWIRSLDLIKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNX0</accession>
    <name>A0A133VNX0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetylglutamate kinase</fullName>
            <ecNumber evidence="8">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">NAG kinase</fullName>
            <shortName evidence="8">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argB</name>
        <name evidence="10" type="ORF">AKJ58_00815</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-acetyl-L-glutamate + ATP = N-acetyl-L-glutamyl 5-phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="8"/>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNX0"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.401"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000004">
        <property type="entry name" value="Acetylglutamate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 8" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="60"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KXB08144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="284" mass="30438" checksum="50CD0CFB526D0080" modified="2016-07-06" version="1">MKRRASVLLEALPYIRRFHGKILVLKLGGALMEEKLMDSICQDIVLLNYVGMKPLVVHGGGSDISEEMEKAGKKPKFIEGLRVTDEETMDIIYKSLGKINKKIVLGLGKHGGKSIGISGIDGGLVRAKKVEGKGKEKLGLVGEVEEIDASVVNYLINTGYIPVIAPMGADAEGTSLNLNADTVAAELSTSLQAEKFILLTDVPGVLEDQENKNSLIPRITLEDAQKLIEEKTVTEGMITKLKACAKSVKGGVKRAHIINGTTPHALLLELLTESGVGTMIEEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNX3</accession>
    <name>A0A133VNX3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein fwdA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000011">
        <property type="protein sequence ID" value="KXB08123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNX3"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.36"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01304">
        <property type="entry name" value="FMDH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012027">
        <property type="entry name" value="Formylmethanofuran_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03121">
        <property type="entry name" value="one_C_dehyd_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF1">
        <property type="entry name" value="COLLAPSIN RESPONSE MEDIATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006453">
        <property type="entry name" value="FwdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="1">
        <location>
            <begin position="38"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="567" mass="63301" checksum="0A1C7E30F00FD3BD" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNY3</accession>
    <name>A0A133VNY3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNY3"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="61" mass="6856" checksum="23D56630E46C62DC" modified="2016-07-06" version="1">MMEGMRERNIPLGDEIGRRARVLDSEMSAFERMKIKKSTIYRLAAASLVYAGSMALMAAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VNZ4</accession>
    <name>A0A133VNZ4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1028 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VNZ4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010430">
        <property type="entry name" value="DUF1028"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39328">
        <property type="entry name" value="BLL2871 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39328:SF1">
        <property type="entry name" value="BLL2871 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06267">
        <property type="entry name" value="DUF1028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="21685" checksum="4D73BD7764B38541" modified="2016-07-06" version="1">MTFSIVARKNKSLGIGVVSGSVAVGDRVPWMKGGVGAIATQGYTETRYGREGLRLLEEGLGPSYVLKKLLQEDFSPERRQVAILSADGRKAVHTGSFCPPTRSSEDRKNYICIGNMLKSERVIPKMAEAFGKRGKLWKRILSALEAGAEAGGDRRGDQTAAILVRNEESLDVKIDYSQNSLRDLRKGILEALSVMNSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP00</accession>
    <name>A0A133VP00_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP00"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.383"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8542" checksum="B28F08273E849C98" modified="2016-07-06" version="1">MVRKKKLSPSGVKGEDGKYHNAHVSLNEDELNAAGLKIGDEVFIRVREDMIIIQKAEEWPERKPIFVERIPVTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP14</accession>
    <name>A0A133VP14_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AKJ58_00670</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP14"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01942">
        <property type="entry name" value="ribokinase_group_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="5">
        <location>
            <begin position="5"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KXB08178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="304" mass="33376" checksum="07D06534909166A1" modified="2016-07-06" version="1">MDSSDVVGFGALNIDRLYRVNRIAREDEESVVTGFSESCGGSAANTIIGLAKLRLKTGYIGKLAEDRDGRLHLEEFKREGVDTRGIIIARNGRSGAVTGFVDKDGERALYIDPGVNDAIKFDEIDLKYAKKAKFLHLTSFVGEKSFEAQKMLMAELPDNVKVSFDPGILYARKGLNSLKPLIEKAFVMLPSERELELLTNEGYENGAKILIEKGVNIVAAKLGERGCYVADRRGGRLVDPYHVKVVDTTGAGDAFSSGFLYGLVQEKGLYECGKMGNYVASRCIQETGAREGLPTRSDLENNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP19</accession>
    <name>A0A133VP19_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP19"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="162" mass="19024" checksum="94B298A6B12D8F54" modified="2016-07-06" version="1">MRDNLKRVCIIFPEDLIQLADEYKNKYHGSRSQLLREALIEYVERLEKEEDERAEMRPLVRKLEDLGKSVERIDNKVGKFRSEAEFSSGGSQKGTPKLASEIERLLLEKGEALTVPEMGEYLPYEQEELIEGVEWLEENFAVERIEPKNVLTKWRIRGYEYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP22</accession>
    <name>A0A133VP22_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">argininosuccinate synthase</fullName>
            <ecNumber evidence="3">6.3.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AKJ58_00835</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="3"/>
    <dbReference type="EMBL" id="LHYK01000010">
        <property type="protein sequence ID" value="KXB08141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP22"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.405"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1260.10:FF:000007">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="10">
        <location>
            <begin position="75"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB08141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="297" mass="33306" checksum="D456C49DEDB7A158" modified="2016-07-06" version="1" fragment="single">AKLVEIAKNEDVDAVAHGCTGKGNDQFRFDTTIALKATEMKIIAPVREMNLDRKWEIKYAKDHNIPIPVDIDKPYSIDENLWGRSIEGGKLEDPKTEPPEEIFELTRAPSEAPKEPQKVEIGFEGGTPIELNGNKKDGAALIKELNQIAGKHGVGRIDTIEDRALGLKSREIYEAPAATVLLKAHKGLEQLVLTREELKFKESVEGLWSDLVYRGMWFDPLREDLDAFVDKTQERVTGKIKVKVDHGSAAVVSRESPFSLHEPELVSFDKFGFDQRESASAIKFQALQGRLYRKLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP26</accession>
    <name>A0A133VP26_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="10">ASA dehydrogenase</shortName>
            <shortName evidence="10">ASADH</shortName>
            <ecNumber evidence="4 10">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">asd</name>
        <name evidence="13" type="ORF">AKJ58_00780</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="4 10"/>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP26"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.393"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="12">
        <location>
            <begin position="5"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="10 11">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 11">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="180"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KXB08167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="355" mass="38953" checksum="9B7A83EF6909812E" modified="2016-07-06" version="1">MTKTPVAVLGATGMVGQRFVQLLANHPYFEIEAFTASKQSVGKRYKEAAKWYLHPPMPEEVKDMEVQPTEEKAVEGAEIVFSALPSNVAKEVEPVMAEAGHLVASNASSYRMESDVPLLIPEVNPEHLALLYEQHDRRGWEGSIVTNPNCTTIILVLALKPLFDDFGVERLFVSTMQALSGAGYGGVKSMEIQDNLIPYIGKEEWKTENETLKILGEFKNGEILPAKIKISSSCHRVPVLDGHTEAVFLELRKETSLEEVSESFAKFKAKPQELKLPTAPEQPIIVETDANRPQPRSARFAGSVEGMAVAVGRIRKDPILKNGIRFVVSGNNAIRGAAGASVLNAELMKSEGLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP32</accession>
    <name>A0A133VP32_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ParB/Sulfiredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP32"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39816" checksum="41304688CC1465E2" modified="2016-07-06" version="1">MNVTPESTTKAELMEVPLPLIRLDPNNVRFCHISSELKDVQMEQLIWKFPATKRLYRQIKWSQGLQEKPILKGEGDHFIVKEGNMRIVCLRKLKQEIEFGDLDSDGFEIDPVRCIVLPKQVSEGEEALLLSRVHVKGKAQWGAFQKAAHIFDLTEKHGFGYADISEGIGVSQVKAKRMKRAFENMLKYERDHDDERWMEKYSYFYELEKKRYSKDKKNPLPKGWVEKNLGEFMEWIHTNQIEKGEEVRELPKIVGNEDAYQCLRDGGTVQEALQVLAQYDPTAGNKIFSRLARLREVINSFRKSEEDKIDAANNPARFNFLKELHKDIDNLISEVEKVKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP40</accession>
    <name>A0A133VP40_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Methionine aminopeptidase</fullName>
            <shortName evidence="8">MAP</shortName>
            <shortName evidence="8">MetAP</shortName>
            <ecNumber evidence="8 9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">map</name>
        <name evidence="11" type="ORF">AKJ58_00740</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8 9"/>
    <dbReference type="EMBL" id="LHYK01000009">
        <property type="protein sequence ID" value="KXB08161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP40"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.384"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="10">
        <location>
            <begin position="9"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KXB08161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="299" mass="32968" checksum="6AAA713D142E8D26" modified="2016-07-06" version="1">MLSQEELKAYREVGKLAGKVREDALELVKPEQKLLTIAEKAEDLIRKGGAEPAFPCNISLNEIAAHYTPPANDETKIEKGDLVTVDIGAHLDGYIGDTAATVSVGGKEDQKIVKAVQQVLEKAIEAANPGVNVGEIGGVIESAAKENGFKPIKNLTGHSLDRWKLHAGISIPNVEKESEEELKEGDVIALEPFITYGAGEVEDKPEIYIFRYLSDANVSGRMPRQTLRRIKKKYGHLPFAERWLAKDMSKIRLKMTLSELITSRSLHPYYVLKEKKGGKVAQAEHTIIITEDGSEVTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP43</accession>
    <name>A0A133VP43_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="8">FGAR amidotransferase I</shortName>
            <shortName evidence="8">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutaminase PurQ</fullName>
            <ecNumber evidence="8">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purQ</name>
        <name evidence="9" type="ORF">AKJ58_00665</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EC" id="3.5.1.2" evidence="8"/>
    <dbReference type="EMBL" id="LHYK01000008">
        <property type="protein sequence ID" value="KXB08177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP43"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.369"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002252">
        <property type="entry name" value="PRK01175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KXB08177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="272" mass="30778" checksum="AB21DB902776603C" modified="2016-07-06" version="1">MVDKVRVCILRVGGTNCDIETKATLDDFVGVDPEIVHINHIGRDRDLMDYDALVIPGGFSYGDHIRAGVVFAKRIITTLSEELRMFVEEERPVLGICNGFQVLIEAGFLPGFEGPSEFPTAALAINESVKYECRWVHLRNENEGKCIFTRELGKGENVFLPVAHKEGRFIFPRKLQDEFLERLLINDQLVFQYSNEQGDPAEGRYPENPNGSFHDIAGICDPTGTILGLMPHPERAYYGWQRPDWTGHKNVLNHADGRVIFESLVEYLLKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP46</accession>
    <name>A0A133VP46_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA repair helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP46"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.366"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09179">
        <property type="entry name" value="PLDc_N_DEXD_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="269"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="527"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="82006" checksum="78DD83E53B84C00F" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP59</accession>
    <name>A0A133VP59_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000006">
        <property type="protein sequence ID" value="KXB08230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP59"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="188" mass="22278" checksum="866EB75E75CCF7F6" modified="2016-07-06" version="1" fragment="single">RKKKIRSFIQSAVSKISRPMRKMKKLIQNKKHAVERDTVEGLKLYSKDPFKAVMSEKEGLPKFGALLRGLKELMENEMKLSRKERQKRSKHVQNLLEDKTLTKLRTQYEEEKKKRLKLDEKIKGSPLLERMDKLKESIQKSKKNLKTAQNDLKMTKEKYAKTQDQIEEKTNKLKNALKSRLRLRVTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP65</accession>
    <name>A0A133VP65_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP65"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="184" mass="21614" checksum="D155329EA07B3F3A" modified="2016-07-06" version="1" fragment="single">KEGWIDWNEGIIKIPGHEPCDCSYCQERARKKKGDYEEVLERQWEPKTKAGAREIPFWHVDGVGETLKEFMSEHGGWPYSMDTMEHRVKELGRLISLDPDRAYPHALRGTGGWKWARWVDGNPFELKVVMGWKNIDMALKYIKGAMLNGTLEEKFDRRGKRWKGLGRVLHTPEKREKLMEMRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP70</accession>
    <name>A0A133VP70_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AKJ58_00550</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000006">
        <property type="protein sequence ID" value="KXB08228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP70"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050545">
        <property type="entry name" value="Mycobact_MmpL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406">
        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406:SF13">
        <property type="entry name" value="MEMBRANE PROTEIN YDFJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="598"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="691"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="724"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="233"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="618"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KXB08228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="752" mass="82422" checksum="103CC385B77F91EE" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP73</accession>
    <name>A0A133VP73_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000007">
        <property type="protein sequence ID" value="KXB08207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP73"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="141" mass="15869" checksum="9AE934CFB49F61B1" modified="2016-07-06" version="1">MASKPPDRVYIDESDKEDYKDLLEDSNSPLEGTGNPDVFLLAAVTGFRKGERIELDSKYGWARTEYFDDKAKSVIKAIAVAEEGLDVLLDKKKVYSIAEEYATTGVKLLKNDIFGRKYGTYIKELESDLKDIFESLSKGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP76</accession>
    <name>A0A133VP76_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP76"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="78" mass="9156" checksum="3EFAEF88334B873F" modified="2016-07-06" version="1">MRKSGLNKSELEAVLTLLLAKIQVLPIEDYDRSPRQFLAKERWKNYQDRVQVRNNLFMMGMIQSKGGDDGWSVNFDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP85</accession>
    <name>A0A133VP85_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribbon-helix-helix protein CopG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP85"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="5"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="95" mass="11282" checksum="BA84515DB6321110" modified="2016-07-06" version="1">MSDETITVRIEPKWKKKIEKLAAEKRETKSDVIREALVEYTQREEERKEIERVVAKKFASEKISFEELTRIVGYDKARKIAFYVQVAKRSFEEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP89</accession>
    <name>A0A133VP89_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="LHYK01000006">
        <property type="protein sequence ID" value="KXB08227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP89"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF1">
        <property type="entry name" value="3-HYDROXYPROPIONATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="442" mass="48186" checksum="6B6D288417EB883B" modified="2016-07-06" version="1">MSLKKLFKPKSLAIIEASAEPDELGHLILKNIIDGGYKGEIYPVNPESEEILGLTCCPSIEELPENVEMAVVVTAAQDVPSTMKQCAKKGIENAVVMSGGFSEVGDEGKDLERELEEIIEEFNLHVLGPNCRGINNTVQNICASWPLITKKGPISIISESGSIGSTLESRASRDNIGISKFASLGNRIDIDESDLLRYFCKDRKTKVIALYIEGLKKGRKFLEAAEKAVKKNNVVMLKGGQSKRGSAAVTSHTNSSGGRYEVFKSVCKRAGILRVDDLDELYDVCKGIANLPKPKEKKTVIITNSGGAGILTVDAGEKLGLDLIDLPKKSVKRLNEKLPPGCVLKNPLDLTSNASAEIYDTAIKVLARYKDVRCIVIIVRDSLLGIADVLKKRFERATIVPVMLEDGNVESEEKRKLQKAKIPVFSSPKRAMKVLSLLPIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP91</accession>
    <name>A0A133VP91_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gfo/Idh/MocA-like oxidoreductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP91"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9922" checksum="285FFE49D29CF0A2" modified="2016-07-06" version="1">MDIFGSEGCMSVDTTTSLPLQIYTGKEASIPDLLRTPRFWIADEVDHFLKCILHNQEHMCTAEEGKKVLEIALGARESAEKHEKVVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP94</accession>
    <name>A0A133VP94_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribbon-helix-helix protein CopG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP94"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.120">
        <property type="entry name" value="Antitoxin ParD1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022789">
        <property type="entry name" value="ParD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038296">
        <property type="entry name" value="ParD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03693">
        <property type="entry name" value="ParD_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="8116" checksum="287E65BC3E586174" modified="2016-07-06" version="1">MSRTKNVSFKATEEQIEQIERAVKSGRFTSRGEFLRSLLRKVEDAELSEKAKSDIEEARKQEGKPLEELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP95</accession>
    <name>A0A133VP95_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP95"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="3"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="84" mass="9424" checksum="D3582556EF72021D" modified="2016-07-06" version="1">MVESIVKIGKRGQITLPKEIREKEGLKEGSFLEISDIGGVLTLRKIEKKPSALDLFKEVGEALKKEGYTRETILELVKEPRKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VP97</accession>
    <name>A0A133VP97_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3368 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VP97"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021799">
        <property type="entry name" value="PIN-like_prokaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39550">
        <property type="entry name" value="SLL0658 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39550:SF1">
        <property type="entry name" value="SLL0658 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11848">
        <property type="entry name" value="DUF3368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18800" checksum="69A8EB4C1DB607FD" modified="2016-07-06" version="1">MVLVLDSTPLIYLGKSDAPEFLEVLDEKRVTSQTVHDEVVKKGKKIGTDDAFLVEKLFEEEKIKIEPIENKKFLENLVKIPGLHMADAEVLALARRLGGTAIIDDEKARNVADLKGIPNHGTAYLLLKLEETGRISKEEARKTLDEMIKEGWKCSTEIYAEILKALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPB0</accession>
    <name>A0A133VPB0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPB0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="78" mass="8441" checksum="F004DB9A05C27E42" modified="2016-07-06" version="1">MPEKACKSPSETKKNREKDPESGTVRGAVCADCGASVVKMADPRKEGTSFFCTKEKKKVNIVSLCSKLAKGFRRSSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPB7</accession>
    <name>A0A133VPB7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPB7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="64" mass="7842" checksum="11F4082E19CDE9C1" modified="2016-07-06" version="1">MVIADAMIWVRSMHMNDWKLVPVVNWSKEYHEAKDFLKDYPELEEFFKYKPTKEFWKTMLEGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPC5</accession>
    <name>A0A133VPC5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-threonylcarbamoyladenylate synthase</fullName>
            <ecNumber evidence="3">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AKJ58_00450</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUA5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3"/>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPC5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.323"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="12">
        <location>
            <begin position="14"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KXB08268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="213" mass="22699" checksum="643C1C7C0609583F" modified="2016-07-06" version="1">MVEVAEVDSEAPSRSVIERAARIIKEGGLVIYPTETVYGLAADARSDEGVARVFEAKSRPLENPISVAVSSLEMAGEAGEISSRAGRLFREFMPGPLSIIVKARSSLSKLLSAGTGKIGIRFPDHLAAQKLIEEFGEPITSTSANISGRPAPFTAQDALDQLGDHVDFAIDSGSVRICRPSTVVDATGEELEIVREGPISVEKLKAALDRPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPC6</accession>
    <name>A0A133VPC6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPC6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="166" mass="18783" checksum="C5B14E729AAF85E2" modified="2016-07-06" version="1" fragment="single">MKKKGVANSVIIIAAVAIIVVVVAGIFLKIGPKASTDQFLTLEEASKIVNDNIINPDELTYHLTTYCWPELLENGDVVASYGNPDHSHVVEEDTWFFWINDFPLIGFSHPTRYVFVNASSGDYWISIEDWWPCLNGEGLWDTPEEYKDNKYLVFSSLTYSQTPIAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPD0</accession>
    <name>A0A133VPD0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Condensin complex subunit 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPD0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19924" checksum="ED6C2EAAC19179AB" modified="2016-07-06" version="1">MKKILQKLKGGDLRSIGRADEVVEDVLEDQQLFEDVFEGILADDPVVAMRASDVVEKVSSKHPEYLQPYKDRLIREISKVERKEVRWHVAQMFSYLEVSKDERNEIIKILNSYIDSSDSAIVKTFSMQTLADFAKKDERIRSRILSKIEKLVETGSPAIVSRGKKLIDKLKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPD4</accession>
    <name>A0A133VPD4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000005">
        <property type="protein sequence ID" value="KXB08261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPD4"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002850">
        <property type="entry name" value="PIN_toxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34610">
        <property type="entry name" value="SSL7007 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34610:SF3">
        <property type="entry name" value="SSL7007 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="147" mass="17222" checksum="AF1C94E6A06225D1" modified="2016-07-06" version="1">MKIVLDTSVFVPTFLDFAEESKTPRVKIFRALQNKSHKLVLSNELESQILRVVNRVEDKDFAGLFRHVLWSDFSIEYVIIEEHQDLRKKWREKVPRKDLDIFITAIAGNADALISEDENLVKKARVLSKDLEVMTPQEFVKEHHHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPD7</accession>
    <name>A0A133VPD7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000004">
        <property type="protein sequence ID" value="KXB08288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPD7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="78" mass="8907" checksum="D9AFE1C852CB4176" modified="2016-07-06" version="1">MSDGKKECPYCGKKYKGWEKHIKSQHPTEPWPPITFGNSQPCDVCGDKPTRVAYSDDNDEIKYLCKAHSDDTEASRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPE0</accession>
    <name>A0A133VPE0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TFIIS-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00195</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPE0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10511">
        <property type="entry name" value="Zn-ribbon_TFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="6">
        <location>
            <begin position="54"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="97" mass="11111" checksum="CB15D9C1A72046F1" modified="2016-07-06" version="1">MYPKKEDGKVSLVCRACGHVEEASESEDYKIVQEGKSKEKPAVIEEKVEGLPQTNVCCPECGHGKAYWWMQQTRGADEPTTRFYRCVKCGCVWREYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPF5</accession>
    <name>A0A133VPF5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPF5"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.251"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049886">
        <property type="entry name" value="CFI_box_CTERM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041770">
        <property type="entry name" value="CFI_box_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="166" mass="19219" checksum="4CB8ACDCC9299713" modified="2016-07-06" version="1">MVGNQMVEEDEKGENGKKVPFDEIAGVPVVFCLKDEIPMEIVGTEIKETEKKKFIFKTTTEEHKHLKYECPECGRYYLRDLERRRGGGCFIATAAYGSPLAKEINVLRRFRDSYLVYREWGRNAISIYYALSPPIAKIIERSESLKKLVRAFLTPIVKFFREKSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPH5</accession>
    <name>A0A133VPH5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPH5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="157" mass="18073" checksum="3F4873CD2474C90B" modified="2016-07-06" version="1">MRFRIGDKILAFDLIDLDQTVIGLNSDSFTEDGKHIFMADLDGMDLEESRKEARRLIKKFDLPHMLVFQSSEDSHHLYSLTPLTFDELVKITFESKTDYNHKLCLLHNKFATLRVTEKEQKPSSIDFKGEIENGDSERELLDGAKTALFKLTGDMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPI0</accession>
    <name>A0A133VPI0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">albA</name>
        <name evidence="7" type="ORF">AKJ58_00255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds double-stranded DNA tightly but without sequence specificity. Involved in DNA compaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the histone-like Alba family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPI0"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.248"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00285">
        <property type="entry name" value="DNA-binding protein Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003088">
        <property type="entry name" value="PRK04015.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="6">
        <location>
            <begin position="11"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="97" mass="10739" checksum="7FF6F970D25C4074" modified="2016-07-06" version="1">MEKSKDRSTENVIYIGNKPVMNYVLAVITQINKGAKEVILKARGRAISKSVDVTETVRNRFLQNLAIADIVTGTEKIETDERTTNVSTITITLGKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPI5</accession>
    <name>A0A133VPI5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPI5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="89" mass="10040" checksum="8B01207D74F4FC48" modified="2016-07-06" version="1">MIPLPYLTPVGILIFGVTILVLWSLREHLGYMAFSGIALTIGIFVYGFGKVVEMLLGGLVGALSTYQIMKWTKSEVEEWKEEFKKKGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPI9</accession>
    <name>A0A133VPI9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPI9"/>
    <dbReference type="PATRIC" id="fig|1698286.3.peg.245"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="MJ1210-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00162">
        <property type="entry name" value="proteasome assembly chaperone family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27866" checksum="CA2730C666D8F942" modified="2016-07-06" version="1">MIESEVKLFNRPKMEKPLLIEGLPGIGYVGKLAAEHLIEEFEAEKFGEINSPFFPHHVSIDSGILSPAKNEFYLVELEGKDVIILVGDVQATSSEGHYELANKVLDTAESFDVSRIFTLGGYATGQYSKSQPKVFGVVNHPKLLEEYGSDGLVIREGTGPIIGISGLLLGLGSLRSIRGVCLLGETHGMLVDHRSARSVLEALSSMLDFKVDLSKLEEHAKKTESILRRMRKVEKLRKRRKEREEEVSYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPJ0</accession>
    <name>A0A133VPJ0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carbohydrate-binding module family 96 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AKJ58_00120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055372">
        <property type="entry name" value="CBM96"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033679">
        <property type="entry name" value="DNRLRE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24517">
        <property type="entry name" value="CBM96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2035"/>
            <end position="2053"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate-binding module family 96" evidence="6">
        <location>
            <begin position="191"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KXB08347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="2061" mass="229988" checksum="6B836DFC13A717DF" modified="2016-07-06" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPJ7</accession>
    <name>A0A133VPJ7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0145 protein AKJ58_00010</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0145 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPJ7"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.70">
        <property type="entry name" value="Hypothetical protein apc22750. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145_YbjQ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068:SF2">
        <property type="entry name" value="UPF0145 PROTEIN SCO3412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="UPF0145 PROTEIN YBJQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="YbjQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="104" mass="11389" checksum="1ADA0FAF2EBE9E5D" modified="2016-07-06" version="1">MIVVNTDFIPDRKIKETLGLVRGNSVRAKHIGKDIKAAFRGIAGGRVTEYVEMLTESREEALNEMVEKAEEMEADAVINVRFMTSSVMGGAAEILAYGTAVKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPJ8</accession>
    <name>A0A133VPJ8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho termination factor-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPJ8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Rho termination factor-like N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="105" mass="11866" checksum="089BFCD4E987A91E" modified="2016-07-06" version="1">MPTKKELLEKMTVKELKQMARNKDLSGYSTKNKSGLVEMIKDNYLKKEIKAWPKLEKETLKKVEEAPKKLTEPSKPAFTMDPAGIAMAVLIVAILLLYIYVSLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPK2</accession>
    <name>A0A133VPK2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AKJ58_00295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000003">
        <property type="protein sequence ID" value="KXB08331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPK2"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF8">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KXB08331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="189" mass="21467" checksum="16B97738674D0BF1" modified="2016-07-06" version="1">MRVRWLGNACIEMFGEKHVLIDPNFTTEPERKADLVLLTHEHDDHFSKEDYKKFSADACFAAPRASLEKFDLKGRAVKPGDKIDGVEVFESDCWKSKESVSYFYRGVLHAGDSAFFPDIEGVKLIFSACFPDYYEDYVSAFKRLGPDLVVPFHYDPIENLKDAEGLMERLSGEGIACELLDPGEDIEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPM5</accession>
    <name>A0A133VPM5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AKJ58_00075</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPM5"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF0">
        <property type="entry name" value="NUCLEASE SBCCD SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KXB08388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="433" mass="49142" checksum="AAF59C1CB6BB6555" modified="2016-07-06" version="1">MIWASKLKVAIISDLHLGSKWESERQQDPFDQARDAFDSALGLGAELILVPGDIFDDRIPRQEVWAHAMRILSLTQGKEGSGLKFIDTIGKDEKEIPKALFRGVPIIAVHGNHERRSVDLVDPIEMLEAAGLVIRLNRGVVLLDTPAGKVAIHGIGYVPEEHIGDVMEIWNPRPIEDAFNVLMIHQSLGQFVHSEKEEPALTPETLPEGFDLYICGHVHYHSETEARRSPLLFPGSTFRTQLLPIEAEVPKGFFMLDLGDSGLSYEFEKLGSVRDFYYEEKTFNRATVAQIESWIENKIEEKLKKQRKNSEKLPIVRFRLRGSLAKGSARSHLNVEELKKKFAEKILLEISKHELTSPELEEKTKFLKDLSEKQVPIEEIAMEILMSNLKEMNYDGVFDVRALYELLSENKGDRAFNMVSDVIDKLTESELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPM6</accession>
    <name>A0A133VPM6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPM6"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="186" mass="20955" checksum="272E072B0955DF60" modified="2016-07-06" version="1">MTQFSAIGVVGLPASGKTSVVEAMVDLGASRVRMGSVVWKEAKRRGLKINEENVGKVANELRNREGLDVIARRCIPIVRKEGRKSKVVVIDGIRGSEEVKEFEEAFDDYFYLVSVDASEGTRFSRIKARGRRDDSSNWKAFQEKNRRELKWGLEEAMKLADFMIVNEGTLEDLRARAAEIFEKVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPN1</accession>
    <name>A0A133VPN1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AKJ58_00190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000002">
        <property type="protein sequence ID" value="KXB08361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPN1"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KXB08361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="103" mass="11542" checksum="DA0EE4F57611C7F3" modified="2016-07-06" version="1">MALTCSSALNYPHQQFFPTNKFPNKYGGEPKNYNLIIEFGSAYGRVYGRGLGCRFGRSFGGAFGSYFGSQRRNYKKSIEFGSIFGSIFGRSNKNTERFINTGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPP0</accession>
    <name>A0A133VPP0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AKJ58_00050</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily. NifB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPP0"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF13">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="29"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KXB08383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="248" mass="27848" checksum="58B0EB90C5BDDAF6" modified="2016-07-06" version="1">MPSYDPIKVSQKIKKIVVRDDQRKYYRFRKARFYGGIATADCVGCNLQCCYCWSNKPRKRPREVGKFYSPKEVAEKLGSIAMESGLSQVRISGNEPTIGRKHLISTLHELQEHGLKFILETNGILIGADLSYASDIAKFGNVHTRVSLKGCDEKQFSKLTGADPESFELQIRALQNLLDSGGDCHASIVEEFASKEKLDSLKRRLERVDLLLARELELESLILYPHVKKQLKDRGISIPSSSTSSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A133VPP8</accession>
    <name>A0A133VPP8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AKJ58_00040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">candidate division MSBL1 archaeon SCGC-AAA385D11</name>
        <dbReference type="NCBI Taxonomy" id="1698286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>candidate division MSBL1</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="19181" last="19181">
            <title>Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea.</title>
            <authorList>
                <person name="Mwirichia R."/>
                <person name="Alam I."/>
                <person name="Rashid M."/>
                <person name="Vinu M."/>
                <person name="Ba-Alawi W."/>
                <person name="Anthony Kamau A."/>
                <person name="Kamanda Ngugi D."/>
                <person name="Goker M."/>
                <person name="Klenk H.P."/>
                <person name="Bajic V."/>
                <person name="Stingl U."/>
            </authorList>
            <dbReference type="PubMed" id="26758088"/>
            <dbReference type="DOI" id="10.1038/srep19181"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SCGC-AAA385D11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LHYK01000001">
        <property type="protein sequence ID" value="KXB08381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A133VPP8"/>
    <dbReference type="Proteomes" id="UP000070256">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KXB08381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000070256"/>
        </source>
    </evidence>
    <sequence length="382" mass="42325" checksum="9213148C0A7F06D3" modified="2016-07-06" version="1">MTSAMRKKVCIIGGGIVGTSIAYHLRNENVNCTLFEKGKLGSGTSSDSIGGFHMFFGEEWEHDFYSYGWRLYQSWAKDPTSNVSFHRTGRLTIAETENEVTSEVLAPAEFTRGTGHENISILSPKETAEFLPGLDISSITKSIYTGAAGYFDPIELIHEFADKAQDCGVKIMTDMEVKEIIVEKESIQGVVTEDGEFDFDVIINAAGPWAIKVGAMAGVELPLVHTKGQVLVLKPKEEIDRPIPMALFTPKGGYLRDEIGGKIIVGKQVYALDNKLEAGFFDPDRSPSSAEPYFKEFVAREMSSKLPFLSDGEILDEWVGYRTVTPDFVPIIDYSPINGFILSVGYSGRGIILAPMGGRMTARIVTEKPIRRYRGKFQLDRF</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>